CARBOHYDRATE POLYMERS
·
2017
·
IF 4.811
Lingling Fu,Jie Zhu,Shuyan Zhang,Xiaoxi Li,Binjia Zhang,Huayin Pu,Lin Li,Qiang Wang
This work discloses the multi-level structure and thermal behaviors of hydrophobic, propionylated starch-based films as affected by the amylose contents of starch materials used. Scanning electron microscopy results showed that amylose promoted the formation of more compact structure within the film matrices. Also, small and wide angle X-ray scattering analysis revealed that higher amylose content was preferable for the formation of new orders on nanoscale and crystallites. With these structural changes, the viscoelasticity of amorphous short chains was enhanced and the glass transition temperature was reduced by the increased amylose content; but the depolymerization of macromolecules and the decomposition of molecular bonds were postponed, since the increase in starch crystallites restricted the motion of adjacent amorphous regions. Hence, this work provides valuable information for rational design of hydrophobic starch-based films with desired thermal features by simply regulating the amylose content of starch raw materials.
研究领域:
食品科学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2023
·
IF 11.2
Fan Wang,Weiqi Wang,Yuqing Wang,Lili Zhang,Yoshio Okamoto,Jun Shen
Two novel cellulose and amylose derivatives bearing bulky tris(2-benzothienylformate) pendants ( Cel - 3 and Amy - 3 ) were expeditiously prepared by one-step esterification . The fluorescent sensing performance of six polysaccharide derivatives, including Cel - 3 / Amy - 3 , and other four previously prepared benzothienyl- or benzofuranyl-phenylcarbamates of cellulose and amylose ( Cel-1 / Amy-1 , Cel-2 / Amy-2 ), were carefully evaluated using eight metal ions , including Co 2+ , K + , Na + , Li + , Hg 2+ , Ni 2+ , Ca 2+ and Fe 3+ . All six derivatives exhibited excellent fluorescence quenching property to Fe 3+ ions with high sensitivity and selectivity. Especially, the limit of detection of Amy - 2 with benzofuranylphenylcarbamates for Fe 3+ was 3.0 μM, much lower than the maximum contaminant level for Fe 3+ in the drinking water . Additionally, the six bulky derivatives displayed the interesting fluorescence “turn-off” and “turn-on” observation, indicating a desirable reversibility for Fe 3+ detection. The high anti-interference ability was also observed for detection of Fe 3+ on the benzothienyl/benzofuranyl derivatives of cellulose and amylose in the combined system containing Co 2+ , K + , Na + , Li + , Hg 2+ , Ni 2+ and Ca 2+ . It suggested that the obtained polysaccharide derivatives with bulky chromophores possessed good potentials for detection of Fe 3+ as high-efficient fluorescent sensors in diverse applications. The sensing mechanism for detection of Fe 3+ was further proposed based on the Stern-Volmer plots and fluorescence titration analysis.
研究领域:
材料科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2026
·
IF 13.2
Wei Gao,Mengli Li,Ming Miao,Tao Zhang
A predictive linkage between operating variables and amylose architecture is needed because current phosphorylase-based syntheses from non-food cellulose, though valuable, lack a process–structure–property map for design and manufacturing. The dual-enzyme cascade reaction for amylose synthesis was investigated by systematic variation of temperature, pH, enzyme stoichiometry and addition timing, and the cellobiose/maltotetraose primer ratio, coupled with time-resolved monitoring and multiscale characterization. Under the optimized conditions (pH 5.0, 50 °C), varying the cellobiose/maltotetraose ratio from 1:0.125 to 1:32 enabled the Ct CBP– Nicα GP cascade to produce amylose spanning 1821 ± 20.06–69,252 ± 30.02 g/mol, with a maximum yield of 35.84%. The concurrent delineation of quantitative distribution curves for cellobiose, glucose-1-phosphate, glucose, and inorganic phosphate revealed the synthesis reaction to be influenced by glucose threshold and delineated into three stages. The availability of primer was found to be the primary variable in programming chain length, polymorph selection ( V -B → V-C → A-V), morphology (granules → aggregates → films), and thermal degradation and gelatinisation properties. Overall, this study provides a predictive framework for synthesising amylose with controllable structure and properties, while also outlining the pathway and theoretical basis for the large-scale conversion of non-food cellulose into high-value carbohydrates.
✓ 已验证
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2020
·
IF 7.053
Zhi-Hua Zhang,Meng-Fan Li,Fei Peng,Shu-Rui Zhong,Zhi Huang,Min-Hua Zong,Wen-Yong Lou
Oxidized high-amylose starch macrogels (OHASMs) were successfully prepared and, for the first time, used as novel delivery vehicles for nutraceuticals. Oxidized high-amylose starch (OHAS) fabricated by 2,2,6,6-tetramethyl-1-piperidinyloxy (TEMPO) oxidation could crosslink Ca 2+ , Al 3+ , Fe 2+ , Fe 3+ , Cu 2+ , Zn 2+ and Ba 2+ . For OHASM, the oxidation degree (DO) of OHAS had great effects on its morphology and controlled release properties except for the variety and concentration of metal ions. OHASM, formed by Zn 2+ crosslinking OHAS (DO~90%), was suitable for encapsulating various nutraceuticals, such as hydrophobic and hydrophilic bioactive substances as well as probiotics, evidenced by 62.4%, 77.9% and 88.8% encapsulation efficiency for β -carotene, tea polyphenols and Lactobacillus paracasei , respectively. Furthermore, the outstanding pH-responsiveness and resistance of OHASM against gastric acid, bile salt, and digestive enzymes were demonstrated by swelling and simulative digestive experiments in vitro . The development of OHASM in this study suggests an attractive strategy for designing potential vehicles to encapsulate a variety of nutrients in food industries.
研究领域:
食品科学
·
生物技术
·
营养学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2019
·
IF 6.044
Yongbo Lyu,Mengchao Yu,Qisong Liu,Qingmei Zhang,Zhanhong Liu,Ye Tian,Defu Li,Mu Changdao
Many kinds of multi-drug-resistant microorganisms have appeared. Moreover, monotherapy is increasingly no longer adequate for many complicated bacterial infections. Therefore, development of efficient combination antibacterial agent is becoming crucial. Herein, we present a hybrid antibacterial agent with enhanced antibacterial activity and high aqueous dissolubility based on silver nanoparticles and curcumin. The silver nanoparticles were firstly synthesized using oxidized amylose as an environmentally friendly reducing agent and stabilizer. Then, curcumin was added into the above mixture to get the hybrid antibacterial agent. The hybrid antibacterial agent presented high dissolubility in aqueous solution and enhanced antibacterial activity. In addition, the hybrid antibacterial agent presented good antioxidant activity and cell compatibility. Overall, the developed hybrid antibacterial agent has a potential to combat multiple bacteria-induced infections of wound surfaces.
研究领域:
材料科学
·
微生物学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2016
·
IF 4.219
Qing Chen,Haojie Yu,Li Wang,Zain-ul Abdin,Xinpeng Yang,Junhua Wang,Weidong Zhou,Hongtao Zhang,Xiao Chen
Amylose grafted poly(acrylic acid) (Am- g -PAA) was synthesized by graft copolymerization of amylose with acrylic acid . The structure of Am- g -PAA was confirmed by 1 H NMR and FT-IR spectra. The morphology, crystallinity and thermal properties of amylose and Am- g -PAA were investigated by SEM, XRD and TGA, respectively. The highest degree of substitution (DS) of carboxyl group was 1.96 which was obtained after reacted for 1 h at 60 °C. Acrylic acid to anhydroglucose mole ratio for DS was 19.81. It was found that a large number of carboxyl groups were grafted on the backbone of amylose. It was also found that ammonia adsorption capacity of amylose increased by grafting poly(acrylic acid) on the backbone of amylose.
研究领域:
材料科学
·
环境工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Yuying Hu,David Julian McClements,Lufeng Wang,Chunmei Li
Potato starch spherulites (PSS) with different molecular weights were obtained by controlled enzymatic hydrolysis of potato amylose starch, followed by super-heated quenching treatment. The impact of enzymatic hydrolysis on mean particle diameter and molecular weight was observed, ranging from 355 to 57 nm. Structural and physicochemical characteristics of produced spherulites were determined using scanning electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, differential scanning calorimetry and rheological measurements. Rheological analysis indicated that PSS sample was viscoelastic with shear-thinning behavior. PSS was found to exhibit weak B-type crystallinity, similarly to that in native starch. PSS 25% had a higher thermal stability with 128.5 °C melting temperature. Microstructure confirmed there were small spherulites (1–3 μm) formed with hydrolyzed amylose starch, and starch with 15% enzymatic-hydrolysis degree was more beneficial to develop spherulite. These results may be useful in development of starch-based functional ingredients applied in plant-based foods as fat replacers, structure formers, or delivery systems.
研究领域:
食品科学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2023
·
IF 11.2
Fan Wang,Weiqi Wang,Yuqing Wang,Wei Zheng,Ting Zheng,Lili Zhang,Yoshio Okamoto,Jun Shen
Three novel amylose and cellulose phenylcarbamate derivatives bearing bulky para -substituted benzothienyl or benzofuranyl pendants were successfully synthesized as chiral fluorescent sensors through carbamoylation followed by Suzuki-Miyaura coupling reactions. The bulky derivatives showed good enantioselective fluorescent sensing properties toward a total of eight chiral quenchers in this study. Especially, a high enantiomeric fluorescence difference ratio ( ef = 164.35) was achieved on amylose benzofuranylphenylcarbamates ( Amy-2 ) to the 3-amino-3-phenylpropan-1-ol ( Q5 ), an important chiral drug intermediate. It indicated that a favorable chiral environment was effectively constructed by arrangement of bulky π-conjugated benzothienyl or benzofuranyl pendants on the phenylcarbamate moieties surrounding the helical backbone, which is crucial for high-efficient chiral fluorescent sensing. As chiral stationary phases for high-performance liquid chromatography, the bulky benzothienylphenylcarbamates of amylose and cellulose also showed good resolution powers to thirteen racemates , including metal tris(acetylacetonate) complexes, chiral drugs, analytes with axial chirality and chiral aromatic amines , which were difficult to be efficiently separated even on the popular Chiralpak AD and Chiralcel OD. The excitation-dependent chiral fluorescent sensing probably followed different mechanisms from that for chromatographic enantioseparation relying on the dynamic collision of molecules in the ground state. The structure of the bulky derivatives was also investigated by CD spectra and POM microscopy.
研究领域:
有机化学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2026
·
IF 6.2
Manman Lu,Haoyuan Wang,Zhilong Zeng,Ahmed H. Abdullah,Yijia Li,Muhammad Wasim,Min Zhou,Xiong Liu
Polysaccharides have been widely reported to reduce starch digestibility. However, most studies primarily attribute this effect to viscosity enhancement or physical entrapment, while limited attention has been given their role in extrusion systems, particularly in relation to multiscale structural reorganization and interactions with starches of varying amylose contents. This study aimed to investigate the effects of yeast β-glucan (YG) on the physicochemical properties, multiscale structure, and in vitro digestion of extruded corn starch (ECS) prepared from corn starch (CS) with varying amylose contents: 55.79% (CS1), 32.15% (CS2), and 3.58% (CS3). YG was incorporated either before or after extrusion. The effects of YG on ECS varied depending on the amylose content of CS. Compared with ECS alone, YG addition prior to extrusion (ECS–YG) significantly reduced the hydrolysis rate and increased the content of resistant starch (RS) of ECS. This effect was particularly pronounced in ECS2–YG, which exhibited a significantly higher RS content (18.86%, P < 0.05), and a lower estimated glycemic index than ECS2. This was likely due to the balanced amylose–amylopectin ratio in CS2, which facilitated molecular mobility and provided sufficient interaction sites for hydrogen bonding and chain entanglement with YG during extrusion. This mechanism was supported by suppressed water solubility, enhanced hydrogen bonding, increased crystallinity, improved thermal stability, and higher viscosity in ECS2–YG. These results could provide a strong basis for the application of YG in extruded corn–based starchy foods with a lower glycemic index.
✓ 已验证
产品关联
DOI
高影响因子
BIORESOURCE TECHNOLOGY
·
2026
·
IF 8.2
Wei Gao,Mengli Li,Ming Miao,Tao Zhang
A library of 37 Nicα GP mutants was built by computational engineering. • L226Y/V437L/F751H showed 2.10 fold higher activity than WT Nicα GP. • The triple mutant improved thermostability and kinetics. • Cascade productivity increased by 26.5 % at a cellobiose/G4 ratio of 1:2. • Structural analysis linked activity gains to regulatory layer optimization. Converting non-food cellulosic sugars into programmable amylose offers a route to higher-value carbohydrate materials. α -Glucan phosphorylase ( α GP) controls glucose-1-phosphate (G-1-P)-dependent chain elongation in cellobiose-to-amylose cascades. Here, Nicotiana attenuata α GP ( Nicα GP) was engineered by integrating AlphaFold3-based structural modeling, ProteinMPNN, EVcouplings, structural priors, and DynaMut2 filtering. Of 916 candidate substitutions, 37 were selected for experimental validation, and combinatorial screening identified L226Y/V437L/F751H as the optimal triple mutant, with 2.10-fold higher relative activity than the same-batch wild-type (WT) control and clear positive epistasis. The mutant further shifted the optimal temperature from 40 to 45 °C, increased thermal transition midpoint from 56.2 to 58.8 °C, and enhanced catalytic efficiency toward both maltotetraose and G-1-P. In the Clostridium thermocellum cellobiose phosphorylase ( Ct CBP)– Nicα GP cascade, L226Y/V437L/F751H reached 38.86 ± 2.06 % conversion at 72 h versus the previously reported WT value of 35.84 ± 1.04 % at 84 h, increasing apparent productivity by 26.5 %. Docking, CAVER, and molecular dynamics (MD) analyses suggested that these gains may be associated with optimization of structural regions surrounding the conserved catalytic core. This work provides an engineered α GP for amylose synthesis from cellobiose and supports peripheral regulatory engineering as a practical strategy for improving phosphorylase-based biocatalytic cascades. Download: Download high-res image (230KB) Download: Download full-size image
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Xinyu Wang,Qi Zhao,Ruijie Li,Wei Liang,Qian Lin,Li Niu,Wenqing Zhao,Wenhao Li
This study aimed to probe the influence of amylose in starch granules on starch modification. Part of the amylose from sorghum starch was removed through warm water leaching, and the samples were then microwaved. The effects of treatments on starch structure, physicochemical properties, and digestibility were researched. Compared to sorghum starch without partial amylose removal, warm water leaching of amylose resulted in deeper concavity of sorghum starch granules and lower crystallinity, short-range ordering, setback and breakdown values, which disrupted the structure and thus rendered the starch granules more susceptible to microwave radiation, amplifying the advantages of microwave-modified starch. In addition, the ordered arrangement of amylose and amylopectin structures within the microwave-treated sorghum starch with pre-removed amylose were more readily disrupted, allowing enzymes to pass through more readily, thus increasing the fast-digestible starch content and digestibility of the starch. In summary, partial amylose removal can be used as a pretreatment method for modifying and expanding starch utilization in food and non-food industries while providing a new idea for developing baked foods.
研究领域:
食品科学
·
生物化学
✓ 已验证
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Wei Gao,Ru Yang,Mengli Li,Ming Miao,Tao Zhang
The enzymatic synthesis of amylose is a sustainable solution for the valorisation of non-food cellulose. However, the limited thermostability and catalytic efficiency of existing α -glucan phosphorylases ( α GPs) hinders its potential for industrial scalability. In this study, we identified and characterised a novel α GP from Nicotiana attenuata ( Nicα GP) via genome mining. It exhibited superior catalytic performance, with a specific activity of 82.1 U/mg under optimised conditions (40 °C and pH 5.0), and notable temperature and pH stability. Supplementation of Mg 2+ further enhanced its activity by 5 %. Kinetic analysis revealed a catalytic efficiency ( k cat / K m ) of 27.46 mM −1 s −1 for maltotetraose (G4). Furthermore, a dual-enzyme cascade system integrating Nicα GP with Clostridium thermocellum cellobiose phosphorylase achieved up to 28.4 % cellulose conversion, synthesising amylose with tunable molecular weights (6602 ± 47.13–67,154 ± 47.28 g/mol) by modulating ratios of cellobiose/G4. Molecular docking identified the conserved residues (Gly196, Arg706, Lys711, and Lys821) critical for substrate binding and pyridoxal 5′-phosphate-mediated catalysis. Overall, this study provides a new enzyme component for the artificial synthesis of amylose from non-edible biomass, facilitating the widespread application of non-edible cellulosic resources for food conversion.
研究领域:
生物催化
·
工业酶学
·
生物质转化
·
植物生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）