Soft Matter
·
2023
·Vol. 19
·期 30
·5711-5722
·
IF 3.4
Yanrong Cheng,Wenlin Xu,Yaqiong Wang,Xia Guo
The PEG/salt aqueous two-phase system (ATPS) is the most common ATPS, but its application is still limited due to the restricted polarity difference between the two phases and the poor enhancement effect of adjuvants on ATPS performance so far. Unlike the adjuvants used so far, calixarenes can bind ions and molecules via multiple noncovalent interactions. In the present study, a water-soluble calixarene, p-sulfonatocalix[4]arene (SC[4]), was used for the first time as the adjuvant to improve the performance of the PEG 600/(NH4)2SO4 ATPS through multiple interactions. It is found that when the SC[4] and the SC[4]/imidazole ionic liquid ([Cnmim]Br) complex were used as the adjuvants, the formation of PEG 600/(NH4)2SO4 ATPS was enhanced, and the transfer of the extracts (including S-mandelic acid, L-tryptophan, and L-phenylalanine) into the PEG phase was promoted. Moreover, although the single [Cnmim]Br, a commonly used adjuvant, does not promote the migration of the target molecules into the polymer phase, the SC[4]/[Cnmim]Br complex is superior to SC[4] in enhancing the performance of the ATPS because the SC[4]/[Cnmim]Br aggregates enable more binding sites to combine with the extract. Besides, the partition coefficient of SC[4] in the PEG/trisodium citrate ATPS is much smaller than that in the PEG/(NH4)2SO4 ATPS, which is helpful for the recovery of extracts into the citrate phase.
研究领域:
生物化学
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分离科学
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PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2023
·
IF 12.6
Haoyue Lv,Xiaoman Duan,Zhaoyu Han,Haozhen Yu,Biwu Liu
Fluorescent DNA assays are promising in disease diagnosis, environmental monitoring, and drug screening, encompassing both heterogeneous and homogeneous assay types. Nevertheless, heterogeneous assays suffer from tedious washing steps and slow reaction kinetics , whereas homogenous assays require well-designed fluorophore pairs to modulate signal off/on. Herein, we developed a cost-effective and efficient quencher-free fluorescent DNA assay using an aqueous two-phase system (ATPS). Using a strand-displacement reaction, we showed that similar sensing performance could be achieved at a much lower cost. Furthermore, the unique crowding environment in ATPS accelerated strand-displacement reactions by up to six-fold and reduced DNA amplification time from 120 min to 30 min. Our assay demonstrated robust sensing in serum environments and successful detection of miRNA extracted from cells. This innovative assay format has the potential for biosensor development with both heterogeneous readout and rapid reaction kinetics in various applications.
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PubMed
高影响因子
Journal of Environmental Chemical Engineering
·
2025
·
IF 7.2
Bingying Wang,Lingling Zhang,Xiaoxing Lu
Aqueous two-phase system (ATPS) has been considered a promising alternative to traditional water-organic systems in liquid-liquid extraction. In this study, extractions of L-tryptophan (Trp), L-tyrosine (Tyr), vanillin (Van) and theophylline (Thp) under various conditions were investigated using the ATPS formed by 1-(2-hydroxyethyl)piperazinium laurate ([HEP][C 11 CO 2 ]) and dodecyldimethylbenzylammonium chloride (DDBAC). Pseudo-ternary phase diagrams were obtained for the DDBAC/[HEP][C 11 CO 2 ]/brine systems at 25.0 °C. In all the ATPSs, the extracts migrated to the top phase, which exhibited higher hydrophobicity than the bottom phase. Due to the increased hydrophobicity difference between two phases, increasing water content from 0.70 to 0.96 resulted in a decrease of the recovery ( R ) of Van from 96 % to 84 %, while the R values of Trp, Tyr and Thp markedly decreased by at most 54 %. The highest partition coefficient ( K ) was observed with Van. Increasing the molar ratio of DDBAC to [HEP][C 11 CO 2 ] resulted in higher R values of Trp and Van, while the initial concentration of extract and the extraction temperature showed limited effects on the partition behavior. NaCl addition effectively expanded the ATPS region in the phase diagram, due to the enhanced salting-out effect. However, it negatively impacted the recovery of Trp and Van, primarily due to the volume expansion of the bottom phase. The results suggested that the water-rich SAIL-ATPS was suitable for the extraction of highly hydrophobic compounds such as Van.
研究领域:
生物化学
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分离科学
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高影响因子
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2018
·
IF 3.141
Pan Sun,Kun Huang,Jieyuan Lin,Huizhou Liu
PEG-based aqueous two-phase systems (ATPS) for extraction and removal of metal ions from industrial wastewaters is a promising strategy to replace traditional organic-aqueous two phase systems, due to its environmentally friendly advantages and excellent separation efficiency from controllable phase structure and physicochemical properties. However, controversy still remains about the partitioning mechanism of metal ions into the PEG-rich phase in ATPS. The present contribution revealed that a specific hydrophobic interaction was a main driving force for the transferring of Cr(VI) ions into the PEG-rich phase in ATPS. The properties of aqueous media around the PEG molecules and Cr(VI) ions also played a crucial role in the partitioning of Cr(VI) ions in the PEG-base ATPS. The PEG-rich phase in the ATPS exhibited a specific affinity toward poorly hydrated HCrO4– ions, compared to the strongly hydrated CrO42– ions. The difference in the properties of aqueous media in the coexisting liquid phases was response for the partitioning of Cr(VI) ions in the PEG-base ATPS. It was confirmed by combined application of UV–vis spectroscopy and molecular dynamics simulations. The present work provides a new understanding into the microscopic mechanism of the partitioning of metal ions in the polymer-based ATPS.
研究领域:
环境科学
·
化学
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高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2017
·
IF 3.181
Zhenyu Cheng,Haiyan Song,Xueling Cao,Qihui Shen,Dandan Han,Fangli Zhong,Haobin Hu,Yingjie Yang
An effective method of concurrent extraction and purification for polysaccharides from Gentiana scabra Bunge stems was developed by microwave-assisted aqueous two-phase extraction (MA-ATPE). Ethanol/sodium dihydrogen phosphate was employed to form aqueous two-phase system (ATPS) by evaluating the extraction properties of five combinations of ethanol/salt systems. The important factors, namely, the composition of ATPS, extraction temperature, solvent-to-material ratio, extraction time and the particle size were investigated by single factor experiment and response surface methodology (RSM) combined with Box–Behnken Design (BBD). The final extraction conditions were concluded as follows: ethanol concentration 21.73% (w/w) and NaH 2 PO 4 concentration 23.27% (w/w) for the ATPS, extraction temperature 95 °C, extraction time 5.8 min, solvent-to-material ratio 21:1 (mL/g) and particle size 80 mesh. Under the optimum conditions, the extraction yield of polysaccharides were 15.97 ± 0.32% for the top phase, 0.58 ± 0.26% for the bottom phase and 16.55 ± 0.13% for total polysaccharides, and the purity of water-soluble polysaccharides was 78.19 ± 0.42%. Compared with smashing tissue extraction (STE), microwave assisted-extraction (MAE), ultrasonic-assisted extraction (UAE) and heat reflux extraction (HRE), microwave-assisted aqueous two-phase extraction not only improved the yield and purity of water-soluble polysaccharides, but also obtained a variety of polysaccharides. In addition, the chemical characterization of polysaccahrides from both the two phases were carried out by Fourier transforms infrared spectrum (FT-IR) and High performance liquid chromatography derivatization with 1-phenyl-3-methy-5-pyrazolone (HPLC-PMP). The extraction mechanism was also discussed by scanning electron microscope (SEM). Therefore, as an efficient and promising technique, microwave-assisted aqueous two-phase extraction may be a viable option for the simultaneous extraction and purification of polysaccharides from plants.
研究领域:
植物化学
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食品科学与工程
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DOI
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2022
·
IF 6.633
Shaoping Ma,Fenfang Li,Zhijian Tan
Aqueous two-phase systems (ATPSs) have been used in the chiral resolution of various enantiomers in recent years. However, it is difficult to recycle and reuse the phase-forming components and chiral selectors in these applications. Therefore, a completely recyclable ATPS that is based on two temperature-responsive polymers, viz. , poly(MAH-β-CD-co-NIPAAm-DMAEMA-BMA) (P NDB-CD ) and poly(NVCL-AM) (P VAm ), was employed for the chiral separation of mandelic acid (MA) enantiomers. The two polymers can be recovered by simply adjusting the temperature; meanwhile, the polymer P NDB-CD can be used as a chiral selector. The factors that affect the chiral resolution process were also investigated. A maximum separation factor ( α ) of 1.49 was achieved via a one-step extraction under the following conditions: P VAm concentration of 14.0 wt%, P NDB-CD concentration of 9.0 wt%, MA concentration of 5.0 mg/g, and system pH of 4.0. Moreover, the two polymers can be easily recycled after the chiral separation process. The recyclable ATPS used for enantioseparation in the current study can provide new ideas for other applications by using enantioselective liquid–liquid extraction (ELLE).
研究领域:
化学
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材料科学
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分离科学
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DOI
高影响因子
Algal Research-Biomass Biofuels and Bioproducts
·
2023
·
IF 5.1
Xiangxiang Zhang,Junhong Xie,Manlin Yan,Zhinan Liang,Hang Zhong,Lujing Ren,Xupeng Cao,Quanyu Zhao
Lutein is the main component of macular and retinal pigment so that it is essential for eye health. In addition, lutein is also related to various metabolic processes in the human body with strong antioxidant properties. The green extraction of lutein in wet microalgae is critical for the further applications as nutraceuticals and pharmaceuticals. Ionic liquid-based aqueous two-phase system (ATPS) is a green approach for multiple product extraction from microalgae. In this study, the extraction performances of ATPS composed of four different cholinium-based compounds (choline geranate, choline chloride, choline dihydrogen citrate and choline bitartrate) and polyethylene glycol 400 (PPG400) were compared. The choline dihydrogen citrate (Ch DHcit) showed a better extraction ability of lutein than other cholinium-based ILs. After optimizing the addition of cholinium-based ILs and PPG400, the ATPS with the best composition (PPG400: Ch Dhcit: water: algal suspension = 42:26:22:10 wt%) was applied to the extraction of lutein from wet Chlorella sp. Six imidazolium-based ionic liquids (1-ethyl-3-methylimidazole chloride, 1-ethyl-3-methylimidazolium bromide, 1-butyl-3-methylimidazolium bromide, 1-butyl-3-methylimidazole chloride, 1-hexyl-3-methylimidazole chloride and 1-hexyl-3-methylimidazolium bromide) were compared as an adjuvant for the lutein extraction from wet Chlorella sp. The introduction of a small amount of 1-ethyl-3-methylimidazole chloride ([C 2 mim]Cl) improved the extraction efficiency of lutein increased by 9.98 %. In the multiple-times extraction process, the total extraction efficiency of three times distribution reached 102.61 %. This paper provides a new approach to the extraction of lutein from algal biomass .
研究领域:
化学工程
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药学
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生物技术
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环境科学
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DOI
高影响因子
PLoS One
·
2025
·Vol. 20
·期 12
·e0336325
·
IF 2.6
Shuiqing Lin,Jinmei Hu,Hui He,Yaying Yu,Zhengguo Zhou,Lin Zhou
Citrus peels, often considered agricultural waste, are rich in flavonoids with potent antioxidant properties. This study utilized ultrasonic-assisted aqueous two-phase extraction (UA-ATPE) to obtain flavonoid-rich upper phase extracts (UPEs) from the peels of four citrus varieties sourced from different cultivars and regions. Targeted metabolomics was used to analyze the flavonoid compositions in the UPEs, revealing distinct metabolic profiles among the varieties. The antioxidant activities of the UPEs were evaluated through hydroxyl, superoxide, and ABTS radical scavenging assays, while their effects on skin cell viability were assessed using HaCaT and BJ cells. Multivariate statistical analyses, including principal component analysis (PCA) and hierarchical clustering analysis (HCA), identified six key differential metabolites (naringenin, p-coumaric acid, luteolin, butin, vitexin/isovitexin, and ferulic acid) that effectively distinguished the citrus varieties. Among the UPEs, Guangxi mandarin orange exhibited the highest total flavonoid content and the most potent superoxide anion and ABTS radical scavenging activities. However, Meizhou shatian pomelo demonstrated the strongest overall antioxidant capacity, as indicated by the lowest IC 50 values for the antioxidant potential composite (APC). Cell viability assays confirmed that none of the UPEs exhibited cytotoxicity at concentrations of 0.06--1.00% (v/v). These findings highlight significant varietal differences in flavonoid content and antioxidant activity, providing a scientific basis for the utilization of citrus peels in functional cosmetics and other industries.
研究领域:
食品科学与技术
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植物化学
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代谢组学
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抗氧化研究
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天然产物提取
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化妆品科学
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细胞生物学
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PubMed
高影响因子
Journal of Food Measurement and Characterization
·
2024
·Vol. 18
·期 12
·9752-9765
·
IF 2.9
Li Min,He Shanshan,Li Lulu,Zhuang Jiaying,Wang Hongyu
In this work, an aqueous two-phase system (ATPS) based on a green deep eutectic solvent (DES) and inorganic salt is presented and applied for the simultaneous determination of heterocyclic aromatic amines (HAAs) in the marinating juice via ultra performance liquid chromatography (UPLC). First, six different betaine-based DESs were prepared, and betaine/1,4-butanediol (Bet/Buta) was chosen as the suitable extractant for the ATPS. Their structures were confirmed via Fourier transform infrared (FT-IR) spectroscopy and proton nuclear magnetic resonance ( 1 H-NMR) spectroscopy. Subsequently, the factors influencing the ATPS were systematically investigated, and the optimal extraction parameters were obtained with a trichloroacetic acid concentration of 5% (w/v), 0.2 g of Bet/Buta, 0.6 g of an inorganic salt (K 2 HPO 4 ), an extraction time of 1 min and a centrifugation time of 2 min. Under optimal extraction conditions, good linearities were obtained, with the correlation coefficient ( R ) ≥ 0.9995. The inter- and intraday precisions were 2.4–4.9% and 2.9–5.3%, respectively. The recoveries for the targeted HAAs ranged between 91.6 and 111.9%. The limits of detection (LODs) of 5.0–14.6 ng/g were obtained. In contrast to the frequently used solid-phase extraction (SPE) technique, the developed DES‒ATPS method, which has higher accuracy and better precision, has advantages of simplicity, speed, good environmental friendliness and low cost per sample. Finally, it was utilized to determine the four selected HAAs in actual samples of marinating juice to evaluate its applicability. Analytical GREEnness metric (AGREE), a novel greenness assessment tool, was tested to verify that this work was a relatively green analytical procedure. These findings demonstrate that the green and efficient DES−ATPS composed of the Bet/Buta DES and K 2 HPO 4 shows great potential for extracting and enriching HAAs or other harmful substances from liquid food in pilot or large-scale operations. Graphical abstract
研究领域:
分析化学
·
食品安全
·
环境科学
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DOI
高影响因子
Food Chemistry-X
·
2024
·
IF 6.5
Xiao-Hua Zhang,Ming-Xuan Li,Shi-Yu Li,Jie Su,Li-Ying Wei,Yan-Ting Yuan,Peng-Hua Shu,Kewen Tang
In this study, an eco-friendly and designable aqueous two-phase system (ATPS) was developed using natural deep eutectic solvents (DES) and short-chain alcohols. The formation mechanism and influence of various factors on phase behavior were investigated. Optimal extraction parameters were determined through single-factor experiments and response surface methodology (RSM): 45 °C temperature, 45 % n -propanol, 40 % DES, and 0.06 mL quercetin working standard solution. Based on the alternating trilinear decomposition assisted multiple curve resolution (ATLD-MCR) algorithm, a calibration model was established for simultaneous and rapid quantitative analysis of flavonoids in Acacia honey, achieving 74.0–86.6 % accuracy and 0.82–2.20 % standard deviation. Moreover, the green chemistry metrics of development method was evaluated using analytical greenness (AGREE) and compared with earlier published methods in the literature. The results indicated that this novel combination strategy of DES-based ATPS with chemometrics conforms to the principles of green chemistry, and is suitable for extracting active components from plants.
研究领域:
分析化学
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植物化学
·
绿色化学
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DOI
PubMed
高影响因子
PROCESS BIOCHEMISTRY
·
2019
·
IF 2.883
Zhe-Ming Wu,Chang-Ling Hao,Tao Tong,Ren-Chao Zheng,Yu-Guo Zheng
( S )-3-Cyano-5-methylhexanoic acid (( S )-CMHA) is the key chiral intermediate of pregabalin . In this paper, an aqueous two-phase system (ATPS) was developed to extract ( S )-CMHA from nitrilase-catalyzed bioconversion broth. Inorganic salts and hydrophilic solvents were screened to form ATPS, among which an acetone/ammonium sulfate ATPS was investigated in detail, including phase diagram, effect of phase composition and stability of ( S )-CMHA. The maximum product recovery of 99.15% was obtained by an optimized ATPS system composed of 15% (w/w) ammonium sulfate and 35% (w/w) acetone with the removal of 99% cells and 86.27% proteins. The total ( S )-CMHA yield reached 92.11% after back-extraction. The recycling use of ammonium sulfate was investigated, and 93.10% of salt in the salt-rich phase was recovered with the addition of methanol. The results demonstrated the efficiency of the two-step extraction process for separation of ( S )-CMHA.
研究领域:
化学工程
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生物技术
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药学
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DOI
高影响因子
RSC Advances
·
2018
·Vol. 8
·期 44
·25201-25209
·
IF 2.936
Li-rong Nie,Hang Song,Alula Yohannes,Siwei Liang,Shun Yao
A magnetic ionic liquids (MILs)-based aqueous two-phase system (MIL-ATPs) obtained by mixing with a series of inorganic salts, which involves five cholinium MILs with the piperidinyloxy radical anion is reported for the first time. Phase diagrams for the new ATPs were experimentally determined at different temperatures (298.15–318.15 K) and the liquid–liquid equilibrium data for two-phase systems were correlated according to the empirical nonlinear expression. The effects of the types of MILs, temperature and inorganic salts on the binodal curve are discussed in detail. The MIL-ATPs coupled with HPLC-UV analysis was developed in the quantitation of berberine hydrochloride in Rhizoma coptidis. Under optimal conditions, the partition coefficient of berberine hydrochloride was 127.68 with the precision values (RSD%) of 1.40% and 2.83% for intra-day (n = 6) and inter-day (n = 3), respectively. The limit of detection (LOD) and limit of quantification (LOQ) for berberine hydrochloride were 0.023 mg L−1 and 0.077 mg L−1, respectively. The recoveries were obtained in the acceptable range of 97.4–101.2%. Moreover, the content of berberine hydrochloride in the raw material of Rhizoma coptidis was measured as 123.95 mg g−1 with this method. Finally, 99.8% MIL was recovered for cycle application after the removal of berberine hydrochloride by using D101 resin. This study provides a meaningful reference for the application of MIL-ATPs with great prospects.
研究领域:
分析化学
·
中药学
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DOI
PubMed
高影响因子
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