SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2020
·
IF 3.232
Zhengdong Shen,Qinzhen Fan,Qian Yu,Rui Wang,Huan Wang,Xianming Kong
This work aims to isolate and detect pesticide (carbendazim) residue in real food samples: orange juice and kale leaves. The combination of on-chip thin layer chromatography (TLC) and surface enhanced Raman scattering (SERS) spectroscopy was used for the separating and detecting of carbendazim (MBC) from the complex food sample. In order to achieve on-site detection of MBC from real food sample, the portable Raman spectrometer was coupled with TLC-SERS. The porous stationary phase composed of diatomite biosilica is beneficial for SERS enhancement and eluent migration. The experiments exhibited that the diatomite chip was suitable for TLC separation and has not shown SERS background and provided excellent separation efficiency, 10 −8 M silver colloids were appropriate for the SERS measurement on TLC chip. The food sample was directly spotted onto the diatomite chip for TLC separation without any pretreatment. The separation and detection process were finished in less than 5 min, the mixture of pyrimethanil , pymetrozine and MBC could be distinguished simultaneously by TLC-SERS at one diatomite chip. The MBC in orange juice and kale were successfully detected, and a limit of detection (LOD) less than 2 ppm could be achieved.
研究领域:
分析化学
·
食品安全
·
光谱学
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产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL POLLUTION
·
2021
·
IF 8.071
Xia Lv,Jing-Xin Li,Jia-Yue Wang,Xiang-Ge Tian,Lei Feng,Cheng-Peng Sun,Jing Ning,Chao Wang,Wen-Yu Zhao,Ya-Chen Li,Xiao-Chi Ma
Carbendazim (CBZ), a broad-spectrum pesticide frequently detected in fruits and vegetables, could trigger potential toxic risks to mammals. To facilitate the assessment of health risks, this study aimed to characterize the cytochrome P450 (CYPs)-mediated metabolism profiles of CBZ by a combined experimental and computational study. Our results demonstrated that CYPs-mediated region-selective hydroxylation was a major metabolism pathway for CBZ in liver microsomes from various species including rat, mouse, minipig, dog, rabbit, guinea pig, monkey, cow and human, and the metabolite was biosynthesized and well-characterized as 6-OH-CBZ. CYP1A displayed a predominant role in the region-selective hydroxylation of CBZ that could attenuate its toxicity through converting it into a less toxic metabolite. Meanwhile, five other common pesticides including chlorpyrifos-methyl, prochloraz, chlorfenapyr, chlorpyrifos , and chlorothalonil could significantly inhibit the region-selective hydroxylation of CBZ, and consequently remarkably increased CBZ exposure in vivo . Furthermore, computational study clarified the important contribution of the key amino acid residues Ser122, and Asp313 in CYP1A1, as well as Asp320 in CYP1A2 to the hydroxylation of CBZ through hydrogen bonds . These results would provide some useful information for the metabolic profiles of CBZ by mammalian CYPs, and shed new insights into CYP1A-mediated metabolic detoxification of CBZ and its health risk assessment.
研究领域:
药理学
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毒理学
·
计算化学
·
实验生物学
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产品关联
DOI
PubMed
高影响因子
ANALYST
·
2025
·
IF 3.3
Lingling Li,Doudou Lei,Pengyue Song,Qingbin Xu,Lihua Huang,Xiao Ma,Lidong Zhou,Ping Zhang,Weijun Kong
Sensitive detection of carbendazim (CBZ), a widely monitored bactericidal pesticide globally, is critical to reduce risks to human health. In this study, Eu ions were embedded into a rigid metal–organic framework (MOF), to prepare Eu-MOF as a fluorescence signal source, which was subsequently post-modified with polyethyleneimine (PEI) for efficient coupling with anti-CBZ monoclonal antibodies (mAbs). On this basis, a PEI@Eu-MOF-mAbs fluorescent probe-based lateral flow immunosensor (LFIS) was developed for CBZ analysis, enabling dual-mode detection: rapid visual qualitative observation by the naked eye and intelligent fluorescence quantitative analysis by using a smartphone. PEI modification not only effectively improved the zeta potential, fluorescence intensity, quantum yield, and biological coupling ability of the Eu-MOF to mAbs, but also significantly enhanced the stability and anti-interference performance of PEI@Eu-MOF. The introduction of PEI@Eu-MOF-mAbs probes characterized by strong fluorescence emission, long fluorescence lifetime, and low background interference, markedly improved the detection sensitivity of the LFIS platform for trace CBZ. Under optimal conditions, the detection limit for smartphone-assisted fluorescence analysis was as low as 1.3 pg mL−1 with a wide linear detection range of 0.25–125 ng mL−1 for CBZ. The recoveries in spiked Panax notoginseng samples were 93.40–106.0% with RSD <1.69%, indicating outstanding reliability of the newly developed LFIS platform for accurate detection of CBZ. In comparison with seven other common pesticides, CBZ produced significantly weakened and stable fluorescence, indicating good specificity and stability of the developed platform over 4 weeks. Compared with current analytical methods, the smartphone-assisted PEI@Eu-MOF-mAbs dual-modal LFIS platform exhibited advantages of simple construction, easy operation, rapid response, obvious visualization to the naked eye, and intelligent quantitative analysis, highlighting its broad application potential for rapid screening and accurate point-of-care detection of CBZ in a large number of foods and agricultural products.
研究领域:
分析化学
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食品安全
·
生物传感
·
免疫学
·
环境监测
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药物残留检测
·
纳米材料应用
·
即时检测技术
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产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2019
·
IF 2.931
Lantian Su,Song Wang,Lumei Wang,Zhiyu Yan,Haoyang Yi,Dongwei Zhang,Guoqing Shen,Yun Ma
This paper proposes a fluorescent aptasensor for the detection of carbendazim (CBZ) in aqueous solution using CBZ-specific aptamer as sensing probe, gold nanoparticles (AuNPs) and Rhodamine B (RhoB) as indicator, respectively. In the absence of CBZ, CBZ aptamer could wrap AuNPs and maintained it dispersed in NaCl solution basically. Contrarily, the aptamer could specifically combine with CBZ and form a stable aptamer-CBZ complex, leaving AuNPs exposed to be aggregated by NaCl solution. The dispersed AuNPs could efficiently quench the fluorescence of RhoB, but those aggregated AuNPs have poor capability to impair the fluorescent indicator . Thus, the concentration of CBZ could be detected quantitatively through the distinction of the fluorescence intensity . This convenient fluorescent assay for CBZ had a wide linear range from 2.33 to 800 nM and a 2.33 nM limit of detection (LOD). Furthermore, it had high selectivity over pesticides, antibiotics, metal ions and other disrupting chemicals. As for application, the method could determine CBZ in water samples with recoveries in the range of 96.3–111.2%. This fluorescent aptasensor possessed great potential application for CBZ detection in actual aquatic environment.
研究领域:
分析化学
·
环境科学
·
纳米技术
✓ 已验证
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2026
·
IF 5.2
Yuting Luan,Hao Wu,Xiaoyu Jiang,Siyuan Song,Li Li,Yongfu Lian
Accurate and rapid monitoring of carbendazim (CBZ) residues is crucial for ensuring food safety and environmental health. Herein, the electrochemical detection capability of superfine ZrO 2 nanoparticles (s-ZrO 2 NPs, ∼2.57 nm) for CBZ is thoroughly investigated for the first time. These s-ZrO 2 NPs are uniformly immobilized on the surface of arc-graphene (AG), which is favorable both to the complete exposure of active sites and to the mutual charge transport. The optimal s-ZrO 2 /AG/glassy carbon electrode (GCE) exhibits significantly enhanced electrochemical sensing performance toward CBZ, particularly in terms of a low detection limit (0.83 nM), outperforming many previously reported transition metal oxide-based or noble metal-based sensors. In addition, the fabricated sensor demonstrates a wide linear detection range (5 nM ∼ 10.4 μM), excellent stability (over 2000 cyclic voltammetry cycles), and remarkable reproducibility (over 30 days). Moreover, it also effectively quantifies CBZ in simulated real environments. Density functional theory (DFT) calculations reveal that electrons spontaneously transfer from s-ZrO 2 to AG in the hybrid structure, which enables s-ZrO 2 to rapidly transfer the electrons generated during the two-electron oxidation process of CBZ, thereby achieving efficient detection.
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Binbin Zhou,Xinyi Li,Xiaoqi Zheng,Meng Liang,Zitong Yang,An Liu,Liang Chen
A novel electrochemical sensor for highly sensitive and selective detection of carbendazim (CBD) in food was proposed in this study. Initially, a three-dimensional carbon composite material (CCM) was formed by simultaneously modifying reduced graphene oxide and reduced graphene nanoribbons on the glassy carbon electrode (GCE) to enhance sensitivity and serve as a stable sensing platform. Then copper nanoparticles were electrodeposited on CCM/GCE, and cyclic voltammetry scanning was performed in potassium ferrocyanide solution to deposit copper-based Prussian blue analogue on CCM/GCE, further improving the sensitivity of the sensor while giving the ability to self-report. Magnetic molecularly imprinted metal-organic framework materials were then dropped onto the electrode to enhance its detection specificity and adsorption capacity for CBD. The sensor has a wide linear range (0.25 nmol/L ∼ 1000 nmol/L) and a low detection limit (0.08 nmol/L), and the recovery rate of CBD in actual corn flour samples is 99.74 % ∼ 102.58 %.
研究领域:
食品科学
·
分析化学
·
材料科学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Wei Zhong,Feng Gao,Jin Zou,Shuwu Liu,Mingfang Li,Yansha Gao,Yongfang Yu,Xiaoqiang Wang,Limin Lu
In this paper, a novel ratiometric electrochemical sensor for carbendazim (CBZ) detection was constructed by a composite of MXene@Ag nanoclusters and amino-functionalized multi-walled carbon nanotubes (MXene@AgNCs/NH 2 -MWCNTs). The Ag nanoclusters (AgNCs) embedded in the MXene not only could inhibit the aggregation of MXene flakes and enhance the electrocatalytic ability, but also serve as an internal reference probe for the ratiometric electrochemical detection. Moreover, the introduction of NH 2 -MWCNTs can further improve the electrochemical signals of CBZ and Ag, resulting in the enhanced signal amplification and higher sensitivity. Based on these characteristics of the MXene@AgNCs/NH 2 -MWCNTs composite, the proposed sensor exhibits a favorable linear relationship between I CBZ /I AgNCs and the concentration of CBZ ranging from 0.3 nM to 10 μM and a low limit of detection of 0.1 nM. Moreover, the proposed ratiometric electrochemical sensing platform also demonstrates high selectivity, good reproducibility, secular stability, and satisfactory applicability in vegetable samples.
研究领域:
分析化学
·
食品安全检测
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL PAPERS
·
2021
·Vol. 75
·期 12
·6477-6485
·
IF 2.097
Ren Xiaohui,Feng Xiaoxiang,Li Xuandong,Li Xin
A large number of pesticides left in the crops and water affect the environment and human health. The combination of surface-enhanced Raman scattering (SERS) technology and molecular imprinting technology (MIT) was presented to apply to the detection of target molecules in water. However, the thickness of the imprinted layer and the inhomogeneity of the shell package have a great influence on SERS detection. To overcome the above problems, a new method is proposed to control the imprinted layer within 5 nm by taking the large-scale Ag as the core, reducing the amount of reagent, increase the oscillation time of polymerization, and so on. The obtained Ag@molecular imprinted polymers (Ag@MIPs) were applied for rapid detection of carbendazim. Besides, the SERS intensity of Ag@MIPs for carbendazim was higher than that of Ag, exhibiting limit detection as low as 1 × 10 −9 M. Also, Ag@MIPs is used as SERS substrate achieved high selectively and satisfactory reusability. Based on the above, the experiment results proved that the Ag@MIPs with a thin imprinted layer provided a reliable approach for carbendazim detection.
研究领域:
分析化学
·
环境科学
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产品关联
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2022
·
IF 4.831
Li Wang,Waqas Ahmad,Jizhong Wu,Xinnong Wang,Quansheng Chen,Qin Ouyang
The persistence of carbendazim residues in the food chain poses a potential risk to human health. Therefore, an eco-friendly selective and sensitive fluorescence nanosensor was established for carbendazim determination based on molecularly imprinted polymer (MIP) modified upconversion nanoparticles (UCNPs). The molecularly imprinted coating with methacrylamide as a functional monomer and carbendazim as a template molecule grafted on the UCNPs (UCNPs@MIP) constituted fluorescent recognition elements. The fluorescence emission of UCNPs@MIP significantly declined in the presence of carbendazim due to electron transfer induced by its selective binding with MIP cavities. The quenched fluorescence of UCNPs@MIP was recovered once the template carbendazim was eluted from the probe system. Under the optimized conditions, the proposed method offers a good linear correlation between 0.01 and 1 μg/mL, with a limit of detection (LOD) of 0.0036 μg/mL for carbendazim residues. The analytical utility and reliability of the developed biomimetic platform were examined in real food samples with good recoveries (88.790%∼102.675%) and relative standard deviation (RSD) values (0.491%∼3.779%). The method was further validated by a standard HPLC method in terms of student’s t-test (p > 0.05) with no significant differences between the two methods. Hence, the proposed fluorescence sensor has prospects for rapid determination of carbendazim.
研究领域:
分析化学
·
食品安全
·
生物传感
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Ting Wang,Lei Zhang,Jieyuan Zhang,Gaoxian Guo,Xinhui Jiang,Zhengwei Zhang,Siqiao Li
Carbendazim (CBZ), a well-known benzimidazole pesticide, is utilized in agriculture to prevent and cure plant diseases caused by fungi. Residual CBZ in food poses serious threat to human health. Herein, a fluorescent two-dimensional terbium-based metal–organic framework (2D Tb-MOF) nanosheet sensor was developed for the rapid and ultrasensitive detection of CBZ. The 2D Tb-MOF nanosheets, prepared with Tb 3+ ions and 5-borono-1,3-benzenedicarboxylic acid (BBDC) as the precursors, exhibited excellent optical properties. Upon the addition of CBZ, the fluorescence of Tb-MOF nanosheets was quenched because of the inner filter effect (IFE) and dynamic quenching. The fluorescence sensor offered two linear ranges of 0.06–4 and 4–40 µg/mL with a low detection limit of 17.95 ng/mL. Furthermore, the proposed sensing platform was successfully applied to assay CBZ in apples and tea, and satisfactory results were obtained. This study provides an effective alternative strategy for the qualitative and quantitative determination of CBZ to ensure food safety.
研究领域:
农业化学
·
分析化学
·
食品科学
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产品关联
DOI
PubMed
高影响因子
ACS Applied Polymer Materials
·
2023
·
IF 5
Yan Qin,Weihua Su,Guihua Meng,Jianning Wu,Lin Cui,Shengchao Yang,Zhiyong Liu,Xuhong Guo
An intelligent responsive pesticide delivery system has been applied in agriculture to improve the utilization rate of pesticides. When plants are affected by fungi, a large number of reactive oxygen species (ROS) are produced rapidly on the wound surface. Based on this, a ROS responsive star polymer micellar slow-release system was constructed to load carbendazim (MBC) and improve its bioavailability. The star polymer was synthesized by using 3-mercaptopropionic acid, acetone, pentaerythritol (PER), ε-caprolactone (ε-CL), and methoxypolyethylene glycols (mPEG5000, Mw = 5000) and named PER-PCL-TK-mPEG5000. By loading MBC, the drug loading micelle MBC-loaded PER-PCL-TK-mPEG5000 was obtained. The cumulative release amount of MBC-loaded PER-PCL-TK-mPEG5000 containing H2O2 is 1.64 times of that without H2O2 after 120 h release. The inhibitory rate of MBC-loaded PER-PCL-TK-mPEG5000 containing H2O2 against Rhizoctonia solani (R. solani) was 91.6%. The critical micelle concentration (CMC) of PER-PCL-TK-mPEG5000 is 4.37 mg/L, and the result showed that PER-PCL-TK-mPEG5000 and MBC-loaded PER-PCL-TK-mPEG5000 have good stability at 4 °C for 14 d. This study proved that the strategy of designing pesticide vectors according to the target environment can be widely used to protect crops, improve pesticide utilization rate, and protect the environment.
研究领域:
农业科学
·
材料科学
·
植物保护
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产品关联
DOI
高影响因子
JOURNAL OF ALLOYS AND COMPOUNDS
·
2025
·
IF 6.3
Yin Liu,Liang Shuai,Lei Cao,Qian Zhu,Lanlan Fan,Liang Xu,Shixian Xiong
The widespread use of carbendazim (CBZ) fungicide calls for monitoring its toxic residues in food due to human health concerns. Herein, a Ru/CeO 2-x heterostructured composite was constructed by sodium borohydride reduction for the electrochemical sensing of CBZ. The rational design engineers abundant interfacial oxygen vacancies and Ru nanoclusters, which form interfacial Lewis acid-base pairs capable of dual-site recognition of CBZ molecules. Specifically, oxygen-vacancy Lewis acidic sites preferentially adsorb the electron-rich groups(such as lone pairs on amino groups), while Ru-based Lewis basic sites capture electron-deficient carbon atoms within the benzimidazole ring. Such synergistic adsorption and interfacial charge transfer markedly enhance the redox kinetics of CBZ. Consequently, the Ru/CeO 2-x /GCE sensor delivers an ultralow detection limit of 7.7 nM, along with excellent selectivity, reproducibility, and long-term stability. This work provides a new interfacial engineering strategy integrating oxygen-defect chemistry and electronic modulation for designing high-performance Lewis acid–base coupled sensing materials.
研究领域:
环境监测
·
电分析化学
·
纳米材料
·
传感材料
·
食品安全
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）