APPLIED CATALYSIS B-ENVIRONMENTAL
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2026
·
IF 19.7
Xiao-Hui Wang,Yue-Han Qin,Xue Xiao,Yu Chen,Shu-Ni Li
Histidine-functionalized Cu 2 O effectively stabilizes Cu + species under cathodic conditions. • The histidine functionalization enhances the co-adsorption of *CO and *NO x intermediates. • The formation of an interfacial hydrogen-bond network on the catalyst surface accelerates proton transfer kinetics. • The histidine-functionalized Cu 2 O catalyst achieves a high urea Faradaic efficiency alongside a notably high yield rate. Electrocatalytic C-N coupling for urea synthesis from CO 2 and nitrate (NO 3 - ) offers a sustainable alternative to the energy-intensive industrial process. Copper (Cu)-based catalysts are uniquely capable of activating both CO 2 and NO 3 - ; however, the catalytically active Cu + species is prone to over-reduction to Cu 0 under cathodic conditions, leading to poor selectivity and instability. Herein, we report a histidine-functionalized Cu 2 O nanospheres (His-Cu 2 O NSs) that stabilize Cu + under operating conditions. The preserved Cu + electronic structure promotes synchronized adsorption of *CO and *NO x intermediates, while protonated amino groups construct a hydrogen-bonded interfacial water network that accelerates proton transfer. As a result, the optimized catalyst achieves a urea Faradaic efficiency of 42.3% and a yield rate of 41.9 mmol h −1 g −1 at −1.1 V potential, with exceptional stability over 100 h in both H-type and flow cells. In situ spectroscopy and density functional theory calculations reveal that histidine coordination not only enhances *CO adsorption but also lowers the energy barrier for C-N coupling, thereby accelerating C-N bond formation. Meanwhile, the in-situ EXAFS results indicate that Cu + species can effectively be retained after histidine modification. This work establishes a molecular-level design principle for regulating complex multi-reactant electrocatalysis and provides a versatile strategy for promoting efficient C-N coupling reactions. Download: Download high-res image (156KB) Download: Download full-size image Functionalizing Cu 2 O nanospheres establishes a generalizable stabilization-via-delocalization principle providing a blueprint for designing durable organic-inorganic interfaces for electrocatalytic urea synthesis.
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PROGRESS IN ORGANIC COATINGS
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2026
·
IF 7.9
Duoduo Huang,Yinchuan Pu,Qingwen Shi,Hengbing Chen,Jinghao Zhong,Ruihua Tian,Qiaoyuan Deng,Jianxiong He,Saifang Huang
Transparent polymer films are widely used in packaging, displays, and functional coatings; however, their optical performance is readily compromised by fogging and surface contamination. Although superhydrophilic hydrogel coatings can effectively mitigate fogging, their weak interfacial adhesion to polymer substrates limits practical application. Herein, an amphiphilic tannic acid–histidine (TA–His) hydrogel coating was fabricated on biaxially oriented polyethylene terephthalate (BOPET) films through a facile drop-casting method. By integrating sodium alginate–Ca 2+ ionic coordination with TA–His covalent crosslinking, the coating forms a stable multifunctional network with balanced hydrophilicity, structural integrity, and interfacial adhesion. After optimization of TA:His ratio, the modified BOPET film exhibited excellent amphiphilicity, with the water contact angle decreasing from 67.4° to 0° and the oil contact angle decreasing from 37.2° to 0°. The optimized coating also showed effective anti-fogging performance, outstanding self-cleaning capability, and good chemical stability. Benefiting from its simple fabrication process, environmentally benign components, and balanced surface properties, this hydrogel-modified BOPET film shows promising potential for anti-fogging materials, anti-fouling films, and related transparent functional coatings.
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JOURNAL OF PHARMACEUTICAL SCIENCES
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2019
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IF 3.197
Yuqing Wang,Ping Li,Feifeng Song,Xi Yang,Yayun Weng,Zhiyuan Ma,Lu Wang,Huidi Jiang
PHT2, a member of the proton-coupled oligopeptide transporter family, participates in the transportation of small peptides and histidine from lysosomes to the cytosol. It facilitates maintenance of intracellular peptide homeostasis. However, it remains a challenge to elucidate the functional properties of PHT2 due to its localization in the lysosomal membrane. The aim of this study was to explore the transport function and substrate properties of human PHT2 (hPHT2) by transfecting Madin-Darby canine kidney cells with hPHT2 mutants to obtain stably expressed protein in the cell membrane. Using this cell model, we found that the transport activity of hPHT2 reached a maximum capacity when the extracellular pH was 5.5. hPHT2 showed relatively low affinity for Gly-Sar and relatively high affinity for d 3 -L-histidine, with K m values of 428 ± 88 μM and 66.9 ± 5.7 μM, respectively. Several typical substrates or inhibitors of PEPT1 and PEPT2, including valacyclovir , Gly-Gly-Gly, and cefadroxil but not 5-aminolevulinic acid or captopril , were proven to be substrates of hPHT2. However, hPHT2 showed low affinity for valacyclovir with a K m value of 5350 ± 1234 μM. In conclusion, this study established a suitable and efficient cell model to explore the function of hPHT2 in vitro and provided important information on the transport activity and substrate properties of hPHT2.
研究领域:
分子生物学
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药理学
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细胞生理学
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PubMed
高影响因子
Catalysts
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2025
·Vol. 15
·期 2
·185
·
IF 4
Jiacheng Sun,Jingjing Guo,Ling Liu,Yu Liu,Linling Yu,Yan Sun
Esterases have been applied in many industrial fields. However, many esterases have inherent defects as natural enzyme, such as low long-term storability, poor operational stability, and difficulty in recovery for reuse. Herein, two histidine derivatives with different structures, L-benzyloxycarbonyl histidine (Z-L-His) and D-benzyloxycarbonyl histidine (Z-D-His), were used to self-assemble with zinc ions to construct esterase mimics (Z-L-His/Zn2+and Z-D-His/Zn2+) based on a minimalist strategy. Two natural enzymes were used for comparison. It was found that the Z-His structure influenced only the stereoselectivity of the enzyme mimic. The kinetics of Z-L-His/Zn2+followed the classical Michaelis–Menten equation, and its catalytic efficiency was comparable to that of natural enzymes. It was verified that Z-L-His/Zn2+had good catalytic stability over a wide range of pH, organic solvent concentrations, ionic strengths, and incubation temperatures. Z-L-His/Zn2+retained high activity after incubation in different environments for 7 d, demonstrating excellent long-term storage stability. Moreover, Z-L-His/Zn2+exhibited good reusability, retaining 93% of its original activity after six cycles, proving its potential for industrial applications in mimicking esterase.
研究领域:
生物化学
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酶工程
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材料科学
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CHEMICAL ENGINEERING JOURNAL
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2024
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IF 13.3
Ning Yue,Yifan Lai,Jiangjiexing Wu,Qiaochu Zhang,Wei Qi,Rongxin Su
Biosensors provide simple, rapid and sensitive detection of a wide range of analytes. Recently, they have been widely used for the rapid detection of pesticides, which makes up for the shortcomings of traditional detection methods. However, the catalytic activity of some nanozymes is not satisfactory in practical applications, limiting the performance of biosensors. Further development of highly active and stable nanozymes is conducive to improving the sensitivity of the sensors and expanding their application in practical detection. Herein we simulated the amino acid microenvironment of natural enzymes and synthesized His-MIL-101(Fe)-X with different histidine (His) modification amounts (X = 0, 25%, 50%, 75%) by regulating the ratio of histidine and terephthalic acid ligands using a one-step solvothermal method. With increasing histidine doping, the peroxidase-like activity of His-MIL-101(Fe)-X was gradually enhanced, and the sensing performance of the sensor arrays constructed by combining His-MIL-101(Fe)-X with three peroxidase substrates was also gradually improved, with a 25-fold increase in detection limit. The His-MIL-101(Fe)-75% sensor array was not only able to accurately discriminate five pesticides in the range of 2-100 μM, but also to quantitatively identify different concentrations of pesticides. Moreover, the His-MIL-101(Fe)-75% sensor array showed good anti-interference capability and was able to discriminate five pesticides at only 2 μM in soil, lake water, seawater, and apple samples, as well as quantitatively detecting diafenthiuron, which has great potential for practical application.
研究领域:
分析化学
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材料科学
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环境科学
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生物化学
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高影响因子
ACS Applied Materials & Interfaces
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2014
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IF 5.9
Yuting Zhang,Dian Li,Meng Yu,Wanfu Ma,Jia Guo,Changchun Wang
Integration of the advantages of immobilized metal-ion affinity chromatography (IMAC) and magnetic microspheres is considered as an ideal pathway for quick and convenient separation of his-tagged proteins, but rare reports concern the natural histidine-rich proteins. In this article, a novel route was presented to fabricate magnetic microspheres composed of a high-magnetic-response magnetic supraparticle (Fe3O4) core and a Ni2+-immobilized cross-linked polyvinyl imidazole (PVIM) shell via reflux-precipitation polymerization. The unique as-prepared Fe3O4/PVIM-Ni2+ microspheres possessed uniform flower-like structure, high magnetic responsiveness, abundant binding sites, and very easy synthesis process. Taking advantage of the pure PVIM-Ni2+ interface and high Ni2+ loading amount, the microspheres exhibited remarkable selectivity, excellent sensitivity, large enrichment capacity, and high recyclability in immobilization and separation of his-tagged recombinant proteins. More interestingly, it was found that the Fe3O4/PVIM-Ni2+ microspheres also showed excellent performance for removal of the natural histidine-rich bovine serum albumin (BSA) from the complex real sample of fetal bovine serum due to the exposed histidine residues. Considering their multiple merits, this new type of Fe3O4/PVIM-Ni2+ nanomaterial displays great potential in enriching low-abundant his-tagged proteins or removing high-abundant histidine-rich natural proteins for proteomic analysis.
研究领域:
生物化学
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材料科学
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生物技术
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分析化学
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PubMed
高影响因子
Nature Communications
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2023
·Vol. 14
·期 1
·1-12
·
IF 16.6
Li Yuqing,Ma Yeying,Xia Yinzheng,Zhang Tao,Sun Shuaishuai,Gao Jiangtao,Yao Hongwei,Wang Huan
Cyclic peptide natural products represent an important class of bioactive compounds and clinical drugs. Enzymatic side-chain macrocyclization of ribosomal peptides is a major strategy developed by nature to generate these chemotypes, as exemplified by the superfamily of ribosomally synthesized and post-translational modified peptides. Despite the diverse types of side-chain crosslinks in this superfamily, the participation of histidine residues is rare. Herein, we report the discovery and biosynthesis of bacteria-derived tricyclic lanthipeptide noursin, which is constrained by a tri amino acid labionin crosslink and an unprecedented histidine-to-butyrine crosslink, named histidinobutyrine. Noursin displays copper-binding ability that requires the histidinobutyrine crosslink and represents the first copper-binding lanthipeptide. A subgroup of lanthipeptide synthetases, named LanKC Hbt , were identified to catalyze the formation of both the labionin and the histidinobutyrine crosslinks in precursor peptides and produce noursin-like compounds. The discovery of the histidinobutyrine-containing lanthipeptides expands the scope of post-translational modifications, structural diversity and bioactivity of ribosomally synthesized and post-translational modified peptides.
研究领域:
生物化学
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微生物学
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药学
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International Journal of Polymeric Materials and Polymeric Biomaterials
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2021
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IF 2.604
Tianhui Liu,Mei Lin,Fan Wu,Aizhu Lin,Daoshu Luo,Ziyang Zhang
To achieve biocompatibility and high gene transfection efficiency, developing new cationic polymers with natural functional moieties has been regarded as a promising way. In this work, histidine was grafted on chitosan (CS) to produce CS derivatives (HGCS) for siRNA delivery and the HGCS/siRNA complexes were formed by the self-assembly method. The structures of histidine grafted CS polymers were characterized by FT-IR and XRD. The Acid-base titration assay was used to measure their proton buffering capacities and results indicated HGCS polymers have stronger buffering capacity than unmodified CS. The particle size, zeta potential and the stability of the polymer/siRNA complexes were measured by laser particle size analyzer and gel retardation assay. The cytotoxicity of polymers was determined by MTT assays in RSC96 cells. Moreover, cellular uptake investigation into polymer/siRNA complexes demonstrated that HGCS polymers have efficient siRNA transfection ability in B16F1 cells and HeLa cells. These data suggest HGCS polymers as a hopeful non-viral gene delivery vectors.
研究领域:
生物医学工程
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药学
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分子生物学
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纳米技术
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高影响因子
TALANTA
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2018
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IF 4.244
Yucan Che,Hongyu Pang,Huiyu Li,Lei Yang,Xinyao Fu,Shiqi Liu,Lan Ding,Juan Hou
Fluorescent carbon quantum dots are emerging as a new class of fluorescent probes due to their unique optical properties . Herein, we presented a simple, one-pot and effective preparation strategy for copper-functionalized carbon quantum dots (Cu-CDs). The Cu-CDs demonstrated excitation-independent emissions, high fluorescent quantum yield (~ 24.4%) and excellent fluorescent stability. The fluorescence of Cu-CDs could be quenched efficiently in the presence of histidine (His) due to the coordination occurring between surface Cu and imidazole side chains of His. Thus, the Cu-CDs could be used as an efficient fluorescent probe for His detection. Under optimal conditions, the fluorescent signals of Cu-CDs decreased linearly with the concentrations of His over a range of 0.1–15 µM. The detection limit was 30 nM. The proposed method provided obvious advantages of rapid response, convenience, simplicity, high selectivity and sensitivity. Moreover, good results also have been obtained for His detection in real biological fluid samples.
研究领域:
材料科学
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分析化学
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生物化学
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SENSORS AND ACTUATORS B-CHEMICAL
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2018
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IF 5.667
Wenchi Zhang,Xiangheng Niu,Suci Meng,Xin Li,Yanfang He,Jianming Pan,Fengxian Qiu,Hongli Zhao,Minbo Lan
In this work, we proposed a new and facile strategy for the high-performance colorimetric detection of Ag + based on the tunable peroxidase-like activity of Pd nanoparticles (NPs) mediated by histidine (His). Bare Pd NPs possess intrinsic catalytic ability to trigger the oxidation of colorless 3,3′,5,5′-tetramethylbenzidine (TMB) to blue TMBox in the presence of H 2 O 2 . After being decorated by His, the formed His-Pd nanozyme exhibits significantly improved mimetic activity due to its favorable physicochemical characteristics including smaller size, interactions between Pd and His, and better hydrophilicity, and such that an enhanced TMB color reaction is observed. When Ag + exists, it is able to despoil the His modifier from the His-Pd surface via the specific interaction between Ag + and His, resulting in the bald Pd again with weak activity. With this principle, highly sensitive determination of Ag + in a linear scope of 30–300 nM was achieved with a limit of detection down to 4.7 nM. In addition, the colorimetric sensor based on the tunable enzymatic activity of nanosized Pd adjusted by His could provide excellent selectivity for Ag + sensing against common interferents. Reliability of the strategy for practical detection of Ag + was also demonstrated.
研究领域:
分析化学
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纳米生物学
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环境科学
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CERAMICS INTERNATIONAL
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2019
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IF 3.45
Xiaoyu Zhai,Yujie Chen,Yunqian Ma,Yanchao Liu,Jiaxiang Liu
Indium tin oxide (ITO), a representative transparent conducting oxide (TCO), is widely applied in photoelectric devices. In this work, monodisperse ITO submicro-spheres have been prepared using one-step solvothermal method at 250 °C. l -Histidine (L-His), triethylamine (TEA) and dimethylformamide (DMF) were used as shape controller, base agent and solvent, respectively. Significantly, the effect from process variables on the size and shape of submicro-spheres was studied systematically. L-His was used to promote the formation of spherical morphologies. When reaction time was prolonged from 12 to 24 h, nano-size particles eventually grew into submicron particles. After adding L-His, the size of ITO particles became smaller and the crystal tended to sphere-shape from cubic-shape. Meaningfully, the effect from particle size and morphology plays a key role in the resistivity of resultant powders. Compared with the resistivity of larger particles with mixed morphology prepared without L-His, the one of well-crystallized sphere-shaped small particles prepared with L-His was lower. The range of resistivity was between 0.4 and 0.7 Ω cm when 0.05 M L-His was added. Moreover, uniform spheres with a diameter of 0.29 μm have been obtained by one-step solvothermal method with 1 M TEA and 0.05 M L-His, which have potential application in photonic band gap crystals.
研究领域:
材料科学
·
光电设备
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ANALYST
·
2012
·Vol. 137
·期 17
·4005-4009
·
IF 4.23
Yu He,Xue Wang,Jingjing Zhu,Shuhua Zhong,Gongwu Song
In this work, Ni2+-modified gold nanoclusters were fabricated for fluorescence turn-on detection of histidine. The fluorescence of Au NCs was first quenched by Ni2+. Then, the addition of histidine can restore the fluorescence of Au NCs by binding with Ni2+ and removing it from the surface of the Au NCs. This architecture ensured non-toxic, cost-effective, label-free and sensitive detection of histidine. The developed Au NCs-based fluorescent sensor offered high selectivity for histidine over other amino acids. The relative standard deviation (RSD) for eleven replicate detections was 2.7%. The detection limit for histidine is 30 nM. The recovery of spiked histidine in human urine samples ranges from 95 to 104%.
研究领域:
分析化学
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纳米技术
·
生物化学
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高影响因子
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