JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
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2018
·Vol. 18
·期 5
·3543-3547
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IF 1.354
Gu Xiangyu,Gao Jing,Li Xiaohan,Wang Yue
Graphene oxide nanosheets was prepared, functionlized with 3-aminopropyltriethoxysilane and used as the carrier for immobilization of papain. A high immobilization yield and efficiency higher than 80% were achieved at pH 8.0 and 35 °C. The thermal stability of the immobilized papain
was improved compared with the free enzyme. And the immobilized papain retained about 63% of its initial activity after 30 days storage at 4 °C, while free enzyme was only 41% at the same conditions. The immobilized enzyme displayed higher binding affinity to substrate because of the unique
properties of GO. These results indicate that modified graphene oxide nanosheets has a good potential as carrier for immobilization of proteins, especially enzymes.
研究领域:
生物化学
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纳米技术
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酶学
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产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
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2016
·
IF 4.712
Chao Ding,Hanjun Sun,Jinsong Ren,Xiaogang Qu
Chiral D- and L-N-acryloyl aspartic acid (NAsp) polyelectrolyte (PE) surfaces with similar chemical compositions and physical properties but opposite chirality are designed for enzyme immobilization . Enzymes immobilized onto the chiral PE surfaces present high chiral preference, namely L-NAsp PE surface can keep most of the catalytic activity of the immobilized enzymes , however, for enzymes immobilized on D-NAsp PE surface a large decrease in catalytic activity occurred which was 11 times lower compared with L-NAsp PE surface. This phenomenon of chiral effect on enzymes immobilization can be explained by attenuated total reflectance (ATR) and circular dichroism (CD) results. The results exhibited that L-NAsp PE surface could preserve most of the secondary structures of immobilized enzymes while on D-NAsp PE surface with a large conformation alteration. These chiral surface induced differences after enzyme immobilization can be further used for logic operation. These results imply a novel strategy for the design of new enzymes immobilization materials based on the chiral effect and expand the applications of enzymes in biochips , chemical transformations and chiral biodevices.
研究领域:
生物化学
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材料科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF WUHAN UNIVERSITY OF TECHNOLOGY-MATERIALS SCIENCE EDITION
·
2018
·Vol. 33
·期 1
·221-225
·
IF 0.524
Zhu Saisai,Zhu Hui,Xia Ru,Feng Xiaoshuang,Chen Peng,Qian Jiasheng,Cao Ming,Yang Bin,Miao Jibin,Su Lifen,Song Changjiang
Diblock copolymers polystyrene-block-polyvinyltriethoxysilane (PS-b-PVTES) were synthesized via atom transfer radical polymerization (ATRP), which self-assembled into spherical micelles in solvent of THF-methanol mixtures. The self-assembled micelles were immobilized by cross-linking reaction of VTES in a shell layer of micelles. The chemical structures of block copolymers and morphology of micelles were characterized in detail. It was found that the size of immobilized micelles was strongly affected by the copolymer concentration, composition of mixture solvent, and block ratios.
研究领域:
材料科学
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化学工程
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DOI
高影响因子
ENVIRONMENTAL POLLUTION
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2020
·
IF 6.793
Yu Xia,Hainan Luo,Dong Li,Zeliang Chen,Shengshu Yang,Zhengang Liu,Tianxue Yang,Chao Gai
A novel amino-functionalized hydrochar material (referred to NH 2 –HCs) was prepared and used as the soil amendment to immobilize multi-contaminated soils for the first time. The results showed that the application of NH 2 –HCs significantly improved (P < 0.05) soil properties (i.e., pH value, cation exchange capacity and organic content). By introduction of NH 2 –HCs, the contaminated soil showed the highest value of 96.2%, 52.2% and 15.5% reductions in Cu, Pb and Cd bioavailable concentrations and the leaching toxicity of Cu, Pb and Cd were remarkably reduced by 98.1%, 31.3% and 30.4%, respectively. Most of exchangeable Cu, Pb and Cd reduced were transformed into its less available forms of oxidizable and residual fractions. Potential ecological risk assessment indicated that the element Cd accounted for the most of total risks in NH 2 –HCs amended soils. The mechanism study indicated that surface complexation, chemical chelating and cation-pi interaction of NH 2 –HCs played a vital role in the immobilization of heavy metals. Pot experiments further verified that the application of NH 2 –HCs significantly improved plant growth and reduced metal accumulations. The present study offered a novel approach to prepare amino-functionalized hydrochars with great potential as the green and alternative amendments for efficiently immobilizing heavy metals in multi-contaminated soil.
研究领域:
环境科学
·
农业科学
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DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
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2016
·
IF 2.189
Chen Chen,Xue-yan Zhu,Qiao-ling Gao,Fei Fang,Li-wei Wang,Xiao-jun Huang
Functional polyphosphazene cyclomatrix microspheres (PMS) containing glucose and dodecyl moieties were utilized for physical adsorption of lipase. In this regard, Candia Rugosa lipase was immobilized onto the above microspheres through physical adsorption, which exhibited catalytic hydrolysis of glycerol triacetate as the model reaction. As observed under transmission electron microscope (TEM) and scanning electron microscope (SEM), the size of all the microspheres ranged from 400 nm to 1 μm with a mean diameter of 680 nm. Zeta potential analysis demonstrated that the microspheres were negatively charged. The influences of buffer pH, substrate concentration and temperature were also studied along with reusability. The obtained results demonstrated that the immobilized lipase on deprotected glycosylated PMS (D-GPMS) showed the best performance and possessed the highest enzymatic loading activity around 1.0884 mmol L −1 min −1 g −1 , and retained nearly 50% of its initial activity even after 10 cycles of application. Moreover, the lipase-microsphere complex displayed high pH adaptability from 7 to 9 and temperature stability above 40 °C. From the viewpoint of biocompatible PMS support, the GPMS-lipase conjugate have potential applications in field such as food, medicine and environment.
研究领域:
生物材料
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酶工程
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纳米医学
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分子生物学
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环境生物技术
✓ 已验证
DOI
高影响因子
WATER RESEARCH
·
2024
·
IF 11.4
Zhangyi Xu,Ziyuan Huang,Han Li,Shishu Zhu,Zhenchao Lei,Chengshuai Liu,Fangyuan Meng,Jeng-Lung Chen,Tsung-Yi Chen,Chunhua Feng
Iron minerals in nature are pivotal hosts for heavy metals, significantly influencing their geochemical cycling and eventual fate. It is generally accepted that, vivianite, a prevalent iron phosphate mineral in aquatic and terrestrial environments, exhibits a limited capacity for adsorbing cationic heavy metals. However, our study unveils a remarkable phenomenon that the synergistic interaction between sulfide (S 2− ) and vivianite triggers an unexpected sulfidation−reoxidation process, enhancing the immobilization of heavy metals such as cadmium (Cd), copper (Cu), and zinc (Zn). For instance, the combination of vivianite and S 2− boosted the removal of Cd 2+ from the aqueous phase under anaerobic conditions, and ensured the retention of Cd stabilized in the solid phase when shifted to aerobic conditions. It is intriguing to note that no discrete FeS formation was detected in the sulfidation phase, and the primary crystal structure of vivianite largely retained its integrity throughout the whole process. Detailed molecular-level investigations indicate that sulfidation predominantly targets the Fe(II) sites at the corners of the PO4 tetrahedron in vivianite. With the transition to aerobic conditions, the exothermic oxidation of CdS and the S sites in vivianite initiates, rendering it thermodynamically favorable for Cd to form multidentate coordination structures, predominantly through the Cd-O-P and Cd-O-Fe bonds. This mechanism elucidates how Cd is incorporated into the vivianite structure, highlighting a novel pathway for heavy metal immobilization via the sulfidation−reoxidation dynamics in iron phosphate minerals.
研究领域:
环境科学
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地球化学
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DOI
PubMed
高影响因子
Waste and Biomass Valorization
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2024
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IF 2.6
Chen Ziqian,Gao Xiaoyan,Zhu Yihui,Deng Yuefeng,Wang Jianjun,Yang Chengli,Hu Kaishun,He Guanya,Li Dali
Food waste cuttlebone has the potential as the raw material of carrier for enzyme immobilization. In this study, we presented a method for synthesizing CaCO 3 nanoparticles coated chitosan (nano-CaCO 3 @chitosan) using cuttlebone as the raw material in the deep eutectic solvent of betaine and urea. The yield of nano-CaCO 3 @chitosan was 89.9%. The nano-CaCO 3 @chitosan was used for immobilization of α-amylase. The yield of enzyme protein was 65.6%. The loading of α-amylase on these nanoparticles was 49.1 mg/g. The optimum reaction conditions of the immobilized α-amylase were 80 °C and pH 4.0. The residual activity of the immobilized α-amylase was 82.9% after reused for 10 cycles. The raw materials for preparing nano-CaCO 3 @chitosan were almost from cuttlebone. The nano-CaCO 3 @chitosan was regarded as reassembly of cuttlebone. Therefore, food waste cuttlebone was utilized as bioresource. Graphical
研究领域:
生物技术
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食品科学
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酶工程
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DOI
高影响因子
Nanomaterials
·
2019
·Vol. 9
·期 11
·1558
·
IF 4.034
Yu Zhang,Yixin Dong,Jinhua Zhou,Ying’ao Hu,Xun Li,Fei Wang
Ferritin possess favorable properties because its exterior and interior surface can be applied to generate functional nanomaterials, which make them possible for enzyme immobilization and recycling. Here, we report the noncovalent immobilization of a genetically modifiedβ-glucosidase onto the outer surface of synthetic magnetoferritin through the electrostatic interaction of a heterodimeric coiled-coil protein formed by coils containing lysine residues (K-coils) and coils containing glutamic acid (E-coils). The immobilized enzyme was characterized, and its enzymatic properties were evaluated. Furthermore, reusability of immobilized enzyme was demonstrated in aqueous solution under an applied magnetic field. The results showed that magnetoferritin was successfully prepared and it was an excellent support for enzyme immobilization. After three times usages, the retention rates were 93.75%, 82.5%, and 56.25%, respectively, demonstrating that immobilized enzyme possessed good retention efficiency and could be used as potential carrier for other biomolecules. The strategy of enzyme immobilization developed in this work can be applied, in general, to many other target molecules.Keywords:magnetoferritin;enzyme immobilization;coiled coils;nanoparticles;recycling
研究领域:
生物化学
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纳米技术
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酶学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2019
·
IF 4.784
Muhammad Bilal,Hafiz M.N. Iqbal
Naturally-derived biopolymers such as alginate , chitosan, cellulose , agarose , guar gum/guaran, agar, carrageenan , gelatin, dextran , xanthan, and pectins, etc. have appealed significant attention over the past several years owing to their natural abundance and availability all over the years, around the globe. In addition, their versatile properties such as non-toxicity, biocompatibility, biodegradability , flexibility, renewability, and the availability of numerous reactive sites offer significant functionalities with multipurpose applications. At present, intensive research efforts have been focused on engineering enzymes using natural biopolymers as novel support/composite materials for diverse applications in biomedical, environmental, pharmaceutical, food and biofuel/energy sectors. Immobilization appears as a straightforward and promising approach to developing biocatalysts with improved catalytic properties as compared to their free counterparts. Biopolymers-assisted enzymes are more stable, robust, and recoverable than that of free forms, and can be employed for continuous biocatalytic reactions. The present review highlights the recent developments and use of biopolymers and their advanced composites as support carriers for the immobilization of a variety of different enzymes to develop biocatalysts with desired catalytic activity and stability characteristics for emerging applications.
研究领域:
生物化学
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生物技术
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材料科学
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产品关联
DOI
PubMed
高影响因子
BIOMACROMOLECULES
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2023
·
IF 6.2
Yiming Liu,Yan Yang,Yueernisa Tuersun,Wei Du,Yuanhang Xu,Xiaoping Zhao,Ge Zhu,Jingzhi Ma,Ning Lin
As a highly crystalline and renewable natural polymer nanomaterial, chitin nanocrystals (ChNCs) have attracted intense interest in the biomedical field. The structure of a ChNC is composed of an acetylglucosamine unit containing two hydroxyl groups and an acetyl group. The acetyl group can be converted to the active amino group through deacetylation, which is under the condition of maintaining the rod-like morphology and high crystalline property and is beneficial for the following modification and potential application. We investigated the relationship between different treatments and varied crystallinities of the modified ChNC, which obtained surface amino groups and aldehyde groups and retained high crystallinity. The natural biomolecules were covalently immobilized on the surface of the ChNC. The etherification was performed based on the hydroxyl groups. Based on the amino groups and the aldehyde groups, the carboxyamine and Knoevenagel condensation reactions were realized on ChNCs. Finally, natural biomolecule-modified ChNCs showed no or low cytotoxicity, antibacterial properties, and high antioxidant properties, which extended their potential biomedical applications.
研究领域:
生物医学工程
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材料科学
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纳米技术
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生物材料
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产品关联
DOI
PubMed
高影响因子
FUEL
·
2023
·
IF 7.4
Hao Zhou,Lingmei Dai,Dehua Liu,Wei Du
MOF-derived carbon has extraordinary advantages including highly ordered morphology inherited from MOFs and improved chemical stability arising from its carbon nature, which may be a promising immobilized enzyme carrier. Herein, we prepared two MOF-derived carbon materials of different structures for the immobilization of lipase from Eversa® Transform 2.0 and systematically evaluated their application potential in biodiesel production system. Compared with C-C (derived from conventional microporous ZIF-8), 3DOM-C (derived from 3D ordered macro-microporous ZIF-8) exhibited superior immobilization performance with an 145.1% increase in enzyme loading and an 130.2% increase in activity. Moreover, lipase immobilized on 3DOM-C ( [email protected] ) showed better catalytic performance than [email protected] , with faster conversion rate in lipase-mediated biodiesel production. Remarkably, the higher surface hydrophobicity of the [email protected] was proved to be the key factor in its improved catalytic performance. Meanwhile, we found that the affinity between immobilized enzyme molecules and water significantly weakened the hydrophobicity of [email protected] and [email protected] , which could be of adverse effect on their application in biodiesel production. To enhance the hydrophobicity of immobilized lipase biocatalyst, a facile post-immobilization hydrophobic modification strategy was proposed by coating the [email protected] with polydimethylsiloxane (PDMS). The as obtained super hydrophobic [email protected] @PDMS biocatalyst showed excellent catalytic performance in biodiesel production, with a much higher initial reaction rate, which was 3.3 folds of that catalyzed by [email protected] , and 1.8 folds of that catalyzed by [email protected] . This work shed light on the great potential of constructing MOF-derived hierarchically ordered porous carbon for enzyme immobilization in extensive practical applications.
研究领域:
材料科学
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生物技术
·
化学工程
·
酶技术
✓ 已验证
产品关联
DOI
高影响因子
Journal of Environmental Chemical Engineering
·
2021
·
IF 5.909
Abdul Latif,Ahsan Maqbool,Kai Sun,Youbin Si
The present work investigated the immobilization of laccase ( Trametes versicolor ) on Cu-alginate beads by entrapment method and its utilization to degrade bisphenol A (BPA) in aqueous medium. The immobilized laccase demonstrated higher storage stability as compared to free enzyme. Box-Behnken experimental design was used to optimize the immobilization conditions (sodium alginate concentration, CuSO 4 molarity, and hardening time). The optimal conditions were found to be 3% sodium alginate concentration (W/V), 0.141 mM CuSO 4 and 90 min hardening time. Experimental data exhibited that immobilized laccase (0.5 g) could degrade 96.12% of BPA (10 mg/L) at pH 5.0, 30°C, 150 rpm and 1 h of reaction. The degradation products of BPA were identified by LC-MS analysis; hence the degradation pathways were suggested. The toxicity of BPA products was judged by phytotoxicity test. Immobilized laccase could also degrade BPA in real water, hence could be considered as an efficient candidate for removing BPA in water.
研究领域:
环境科学
·
生物技术
✓ 已验证
DOI
高影响因子
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