Journal of Materials Chemistry B
·
2022
·Vol. 10
·期 40
·8274-8281
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IF 7.571
Yahui He,Zheng Zhou,Yuting Huang,Wenxiang Zhu,Ning He,Xiaolong Zhu,Xiaoxiao Han,Hairong Liu
The limited availability of bioinks has hindered the application of 3D bioprinting to tissue engineering, and bacterial infection is a serious threat to these applications. Aiming to solve this problem, a novel ε-poly-L-lysine (EPL)-derived antibacterial bioink has been developed for 3D bioprinting and tissue-engineering applications. Three glycidyl methacrylate (GMA)-modified EPL products, EPLGMA-1, EPLGMA-2, and EPLGMA-3, were prepared by reacting 3, 4, and 5 mL GMA with 5 g EPL, respectively. EPLGMA-1, EPLGMA-2, and EPLGMA-3 were photocurable and their corresponding photo-crosslinked hydrogels, EPLGMA-1H, EPLGMA-2H, and EPLGMA-3H, all exhibited very high antibacterial rates, good biocompatibility, good degradability, and promising mechanical properties. After EPLGMA-1H, EPLGMA-2H, and EPLGMA-3H with encapsulated chondrocytes were incubated for 4 weeks, EPLGMA-3H was the best one among them for tissue-engineering applications due to its most efficient tissue regeneration. Carrying chondrocytes, the EPLGMA-3 solution was printed into hydrogel products with high-fidelity shapes and high cell viability using a projection-based 3D bioprinter. Following the implantation of chondrocyte-loaded EPLGMA-3H samples into nude mice for 4 weeks, cartilage-like tissue was regenerated, suggesting that EPLGMA-3 is a promising antibacterial bioink for 3D bioprinting and tissue-engineering applications.
研究领域:
生物材料学
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再生医学
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组织工程
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PubMed
高影响因子
JOURNAL OF POLYMER SCIENCE
·
2021
·Vol. 59
·期 17
·1958-1971
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IF 0
Guoyang Qin,Cong Hu,Yanfen Jiang,Shuqi Dong,Li Liu,Hanying Zhao
We report the synthesis of pH- and enzyme-responsive amphiphilic diblock copolymers through reversible addition-fragmentation chain transfer polymerization of a lysine-derived methacrylate monomer comprising p -nitrobenzyl carbamate ( p NBC) functionality using a poly(ethylene glycol)-modified macro-chain transfer agent. Depending on the hydrophobic block length, the diblock copolymers self-assemble to form spherical micelles, wormlike micelles, and bilayered vesicles in the aqueous solution. The responsive behaviors of the polymeric vesicles to pH, enzyme, and light are investigated in detail. As the pH lowers to pH 5.0, the polymeric vesicles undergo a morphological transition from vesicles to spherical micelles. In the presence of nitroreductase and a cofactor NADH, the decomposition of p NBC releases the ε-NH 2 of the lysine moiety and hence induces the generation of the vesicles with crosslinked membranes at pH 7.4. Moreover, owing to the degradation of p NBC moiety under UV irradiation, the polymeric vesicles also demonstrate a photo-responsive feature. As the irradiation time prolongs, it is observed a light-triggered morphological transition from vesicles to wormlike micelles with network-like structures. Graphical
研究领域:
材料科学
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生物化学
·
纳米医学
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MICROCHEMICAL JOURNAL
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2022
·
IF 5.304
Dandan Guo,Xiaoqian Zhou,Shaohua Huang,Yan Zhu
In this work, achiral metal organic frameworks (MIL-53(Al)–NH 2 ) were synthesized and functionalized with poly (amidoamine) (PAMAM) dendrimers. By dispersing PAMAM grafted MIL-53(Al)–NH 2 to water, the obtained solution exhibited high fluorescence intensity and stability. Due to the strong affinity interaction, fluorescence of the solution was quenched when Cu 2+ was added. Following the addition of lysine to the system, the quenched fluorescence intensity was partially restored based on the chelation between Cu 2+ and lysine. In this case, the fluorescent signal of MIL-53(Al)–NH 2 -PAMAM-Cu 2+ system became turn-on state. Furthermore, because of different chelating abilities with L-lysine and D-lysine, the system exhibited enantioselective fluorescence enhancement. Therefore, the PAMAM grafted MIL-53(Al)–NH 2 were successfully used for enantioselective fluorescence detection of lysine after being quenched by Cu 2+ . The method showed good linearity in both L/D-lysine detection (R 2 = 0.9976–0.9984) and ee values determination (R 2 = 0.9921). The detection limit of L-lysine and D-lysine were 7.52 and 12.2 μmol L −1 , respectively. Reliability of the method was verified by analysis of lysine enantiomers in spiked drinking water samples. Rapid enantioselective fluorescent imaging of lysine enantiomers showed great application prospects in test paper, diagnostic kit and vivo imaging system.
研究领域:
分析化学
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生物化学
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高影响因子
DYES AND PIGMENTS
·
2018
·
IF 3.767
Zhonghua Xue,Lulu Xiong,Honghong Rao,Xiuhui Liu,Xiaoquan Lu
In this work, we proposed a naked-eye colorimetric strategy for simultaneous detection of cysteine and lysine based on 3,3′,5,5′-tetramethylbenzidine (TMB) system in a neutral condition (pH 7.0). Based of the fact that Ag + could oxidize 3,3′,5,5′-tetramethylbenzidine (TMB) to oxidized TMB (oxTMB) and induce a blue color solution corresponding to an absorption peak centered at 652 nm, thus the adding of cysteine or lysine to formation of different product results in the blue color fading to colorless quickly or change to scarlet, respectively. With this strategy, the limit of detection in experimental measurement for cysteine and lysine is 2.46 μM and 3.87 μM, respectively. And the selectivity can also be guaranteed among the other amino acid. The cost is quite low since the use of TMB and Ag + . More importantly, the colorimetric detection operation is very simple without any further modification process and the reaction under physiological conditions is more meaningful for detection of amino acid.
研究领域:
分析化学
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生物化学
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高影响因子
TOXICOLOGY AND APPLIED PHARMACOLOGY
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2026
·
IF 3.6
Chaocheng Wang,Haiqun Yang,Zhiyu Wang,Junzhao Liu,Yu Du
Neurological damage induced by diquat (DQ) poisoning has garnered significant attention from researchers in recent years. This study represents the first systematic investigation into the molecular mechanisms underlying acute DQ-induced brain injury by integrating quantitative proteomics with lysine succinylation proteomics in male C57BL/6 J SPF mice exposed to 200 mg/kg DQ dibromide via a single oral gavage. The study revealed that DQ poisoning significantly downregulated lysine succinylation levels in mouse brain tissue, thereby inhibiting key mitochondrial energy metabolism pathways and disrupting glycine-serine-threonine metabolism and the one‑carbon folate pool pathway. These alterations led to energy metabolism disorders, exacerbated oxidative stress, and enhanced neuroinflammation, ultimately triggering neuronal apoptosis and neurological dysfunction. This study reveals the central regulatory role of abnormal lysine succinylation in DQ neurotoxicity, providing a novel potential target for clinical intervention.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2023
·Vol. 415
·期 12
·2185-2191
·
IF 4.3
Wang Huaxin,Ai Mimi,Liu Jinshui
Probes for detecting phosphate ions (Pi) are required for environmental monitoring and to protect human health. Here, novel ratiometric luminescent lanthanide coordination polymer nanoparticles (CPNs) were successfully prepared and used to selectively and sensitively detect Pi. The nanoparticles were prepared from adenosine monophosphate (AMP) and Tb 3+ , and lysine (Lys) was used as a sensitizer (through the antenna effect) to switch on Tb 3+ luminescence at 488 and 544 nm while Lys luminescence at 375 nm was quenched because of energy transfer from Lys to Tb 3+ . The complex involved is here labeled AMP-Tb/Lys. Pi destroyed the AMP-Tb/Lys CPNs and therefore decreased the AMP-Tb/Lys luminescence intensity at 544 nm and increased the luminescence intensity at 375 nm at an excitation wavelength of 290 nm, meaning ratiometric luminescence detection was possible. The ratio between the luminescence intensities at 544 and 375 nm ( I 544 / I 375 ) was strongly associated with the Pi concentration between 0.1 and 6.0 μM, and the detection limit was 0.08 μM. The dual-emission reverse-change ratio luminescence sensing method can exclude environmental effects, so the proposed assay was found to be very selective. The method was successfully used to detect Pi in real water samples, and acceptable recoveries were found, suggesting that the method could be used in practice to detect Pi in water samples.
研究领域:
分析化学
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环境科学
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纳米技术
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生物无机化学
·
发光材料
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高影响因子
JOURNAL OF APPLIED TOXICOLOGY
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2026
·
IF 2.8
Zhen Peng,Yimin Cheng
Hexavalent chromium [Cr (VI)] is employed across a broad spectrum of manufacturing processes, resulting in considerable environmental contamination. Cr (VI) has the capacity to accumulate in spermatogonial stem cells, adversely affecting male reproductive function. However, the precise effects of Cr (VI) on human sperm function and the associated mechanisms remain inadequately characterized. We postulated that Cr (VI) impairs human sperm function by disrupting lysine glutarylation and mitochondrial function. This study demonstrates that exposure to K 2 CrO 4 leads to a reduction in sperm lysine glutarylation levels, an increase in intracellular reactive oxygen species (ROS), mitochondrial depolarization, and a decrease in ATP content. Notably, the administration of sodium glutarate, a precursor of glutaryl-CoA, was observed to partially alleviate the detrimental effects of K 2 CrO 4 on lysine glutarylation levels, mitochondrial function, and sperm motility. Collectively, these findings substantiate our hypothesis that the impairment of human sperm motility induced by Cr (VI) is mediated through interfering with lysine glutarylation and mitochondrial function.
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高影响因子
MACROMOLECULES
·
2025
·
IF 5.2
Shangzhong Zhang,Yixuan Xu,Panpan Li,Minghui Fan,Dejia Chen,Chengming Wang,Lifeng Yan
Polymerization of CO2 into polyurea under mild pressure and temperature is still a challenge, especially when using diamine from natural feedstocks. Herein, l-lysine is directly used as a diamine without derivatization and protection for capture and copolymerization of CO2 under mild temperatures and pressures (0.1–0.5 MPa, 60–90 °C) with the assistance of superbase 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU). Water-soluble lysine-polyurea (L-PU) has been synthesized. The weight-average molecular weight of L-PU reaches 3 × 104 g/mol, indicating the efficient reaction of lysine and CO2. In addition, NMR studies confirmed that the unprotected carboxyl acid group (−COOH) in L-PU remains more than 80%, and the polymer may be a candidate to replace nonbiodegradable polyacrylate acid (PAA). The free carboxyl acid group also provides a change to further derivatives of the L-PU. Interestingly, the water-soluble L-PU showed efficient fluorescence performance with quantum yield (QY) up to 20%, which may be attributed to the nonconventional intrinsic fluorescence of nonconjugated aliphatic polyureas containing heteroatoms. It allows the L-PU to be used for cell bioimaging, and in vitro studies reveal that the L-PU is biocompatible and can be efficiently endocytosed by cells with bioimaging ability, indicating that it may be a potential drug delivery system for imaging-guided treatment for cancer tissue.
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2024
·
IF 8.1
Chenxi Tian,Da Lei,Yongchao Qian,Xiang-Yu Kong,Zhong Liu
The development of advanced membranes with precise ion selectivity is essential for efficient Li + /Mg 2+ separation. In this study, we synthesized a novel Lys-TMC membrane via a nucleophilic addition reaction between Lysine and trimesoyl chloride (TMC), yielding in a negatively charged surface interconnected by amide bonds. We systematically investigated the effects of varying Lys:TMC ratios on the membrane’s chemical structure, surface morphology, and ion separation performance. Our results revealed that the degree of cross-linking increases initially with the Lysine content, reaching an optimum at a Lys:TMC ratio of 10:1, where the membrane exhibited prominent Turing patterns on its surface. These patterns were critical for achieving effective ion separation. In contrast, excessive Lysine led to spontaneous polymerization, resulting in irregular ‘stone-like’ structures and the loss of Turing patterns. The optimally cross-linked Lys-TMC membrane displayed superior Li + /Mg 2+ separation performance, highlighting the crucial role of controlled cross-linking and Turing structure formation in enhancing ion selectivity. This work provides a mechanistic understanding of the relationship between Turing structure formation and ion sieving performance, offering a promising strategy for designing advanced separation membranes.
研究领域:
材料科学
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化学工程
·
膜技术
·
离子分离
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DOI
高影响因子
Materials Today Sustainability
·
2024
·
IF 7.1
Weijiong Dai,Jing Zhao,Zongqiang Liu,Yan Wang,Jiajun Zheng,Ruifeng Li
Plate-like TS-1 zeolites offering a short diffusion pathway along the b- axis are highly desirable catalysts in the reaction of alkene epoxidation. Herein, we successfully developed a simple synthetic strategy toward reducing the thickness along the b -axis of TS-1 zeolite crystals, l -lysine adopted as a zeolite growth modifier was firstly proposed to prepare plate-like TS-1 zeolite. Meanwhile, through tuning the preliminary aging time, plate-like TS-1 zeolites with thickness in the range of 80–1000 nm can be controllable obtained. In this work, the synthesis parameters controlling the formation of plate-like TS-1 zeolites are studied, and the factors controlling the zeolite growth were well elucidated. Compared with the conventional TS-1 zeolite as well as the plate-like TS-1 synthesized in the fluoride-containing medium, the obtained plate-like TS-1 shows improved catalytic performances in catalyzing epoxidation of 1-hexene.
研究领域:
材料科学
·
催化化学
·
合成化学
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高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2024
·
IF 12.2
Shang Wu,Jian Ji,Lina Sheng,Yongli Ye,Yinzhi Zhang,Xiulan Sun
Disinfectants are widely used in food production and environmental sanitation to prevent illness, but bacteria resistance to these disinfectants and co-resistance to antibiotics pose a threat to public health. This study investigated the impact of commonly used disinfectants on the resistance of Salmonella Typhimurium (ST) to disinfectants and antibiotics, and explored the metabolic mechanisms underlying the resistance changes. The results showed that subinhibitory concentrations of disinfectants had a minor impact on the resistance of ST to four disinfectants. However, chlorine-containing disinfectants stress enhanced bacteria resistance to ampicillin, while quaternary ammonium compounds stress increased resistance to tetracycline and gentamicin. Untargeted metabolomics analysis revealed significant changes in glutathione metabolism and lysine and valine degradation pathways after disinfectant exposure. Specifically, ST activated lysine decarboxylation, leading to a significant decrease in lysine levels after benzalkonium chloride exposure, while valine and leucine degradation pathways were activated by sodium hypochlorite stress. The addition of downregulated L-lysine and L-valine increased the sensitivity of ST to antibiotics, providing further evidence for the findings of metabolomics. This study provides guidance for the proper use of disinfectants in food processing and establishes a strategy based on metabolomics to control antibiotic-resistant bacteria.
研究领域:
微生物学
·
代谢组学
·
食品安全
·
公共卫生
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PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2020
·
IF 7.182
Hao Wu,Shuqian Hu,Chunling Nie,Jiahui Zhang,Huafeng Tian,Weicheng Hu,Ting Shen,Jianguo Wang
Pathogens in the food and environment pose a great threat to human health. To solve this problem, we described a novel route to synthesize antibacterial epsilon-poly-L-lysine (EPL) anchored dicarboxyl cellulose beads. Cellulose beads were prepared via a sol-gel transition method and oxidized by sodium periodate and sodium chlorite to form carboxyl groups. EPL was anchored on the beads using carbodiimide mediated amidation. The structure and morphology of beads were characterized by FTIR, XPS, XRD, SEM, and TGA. After dissolution and regeneration, the crystalline form of cellulose is transformed from cellulose I to cellulose II. The thermal degradation temperature of the beads is 200∼300 °C.The samples displayed excellent antimicrobial activity against Staphylococcus aureus , Alicyclobacillus acidoterrestris and Escherichia coli within 12 h. The beads could be biodegraded in soil after 20 days. The biodegradable beads exhibited great potential in food and environmental applications.
研究领域:
材料科学
·
化学
·
生物技术
·
环境科学
·
食品科学
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PubMed
高影响因子
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