JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2017
·Vol. 164
·期 9
·B421
·
IF 3.259
Jing Lu,Lin Wu,Yufang Hu,Sui Wang,Zhiyong Guo
A Faraday cage-type electrochemiluminescence (ECL) biosensor for ultrasensitive detection of cancer-associated microRNA-21 was constructed based on Fe3O4@Au magnetic nanoparticles (Fe3O4@Au MNPs) and multi-functionalized graphene oxide. The capture unit (Fe3O4@Au MNPs-cDNA) was prepared by immobilizing capture DNA (cDNA) on Fe3O4@Au MNPs, while the multi-functionalized graphene oxide acted as the signal unit ((sDNA&luminol)@GO). In this biosensor, the capture unit, which was used to catch the microRNA-21, could be easily attracted onto the magnetic glassy carbon electrode surface. After the hybridization of microRNA-21 with the capture unit, the signal unit was further hybridized to form a Faraday cage-type structure, in which the signal unit was directly fixed onto the electrode surface, resembling a huge net and even becoming a part of the electrode surface. All luminol molecules labeled on the signal unit could take part in the electrode reaction, thus greatly enhancing the ECL signal. The ECL intensity was found to increase with the logarithm of microRNA-21 concentrations from 1 fM to 10 pM with a detection limit of 0.3 fM. In addition, this proposed Faraday cage-type ECL biosensor can be used for practical analysis of microRNA-21 in human serum, providing an ultrasensitive detection platform for microRNAs in clinical diagnosis.
研究领域:
生物传感器
·
纳米技术
·
癌症诊断
·
电化学
·
分子生物学
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Huiling Fan,Yuqi Qin,Long Li,Jiansheng Cui,Chenqiang Yang,Zhanhui Wang,Lili Duan,Yihong Wang,Liang Tian
Herein, a biosensing system was designed based on tripedal DNAzyme walker integrated nanoprobe signal amplification strategy for realizing ultrasensitive determination of the microRNA-21. In this work, the two-dimensional MoSe 2 /MXene nanocomposite with high electronic conductivity was synthesized as electrode signal amplification materials to immobilize more capture DNA and accelerate electron transport. After adding microRNA-21, the tripedal DNAzyme walker was activated on the surface of the Pt@CeO 2 nanoclusters, which were as nanozyme to construct the high-performance nanoprobe machine. Then, the tripedal DNAzyme strands could cleave the substrate strands and walk onto the nanoprobe for amplifying the signal remarkably. Furthermore, the detectability of the electrochemical biosensor was verified by DPV, acquiring a wide linear response in a range of 0.1 fM to 1 nM, as well as the limit of detection was 0.04 fM. Hence, such double signal amplified strategy could not only provide excellent selectivity and specificity, but also achieve superior repeatability and stability in real complex serum samples. Therefore, the designed biosensing platform detected different microRNA through modulating the recognition sequence, which might provide a general strategy for various cancer analyses and have a promising potential for clinical application.
研究领域:
生物化学
·
分子生物学
·
医学诊断
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2021
·
IF 6.986
Jiao Yang,Yanzhou Li,Longhua Guo,Bin Qiu,Zhenyu Lin
Conventional photosensitive materials such as TiO2 suffer from restricted absorption in the ultraviolet region, fast recombination of photogenerated electron–hole pairs, and a lack of functional groups for biocoupling, which hinder their application in photoelectrochemical (PEC) biosensing. Herein, a new coordination polymer (CP) based on Cu(I), chloridion, and 4-mercaptobenzoic acid (MBA) has been designed and synthesized (called CuClx(MBA)y). The prepared p-type CuClx(MBA)y exhibits visible-light absorption due to its narrow optical band gap (2.59 eV), and its proper band edge position enables it to form a p–n junction with TiO2. Through layer-by-layer assembling, the photocurrent intensity of the CuClx(MBA)y/TiO2/FTO composite photoelectrode was 3.7-fold higher than that of a TiO2/FTO electrode and 35-fold higher than a CuClx(MBA)y/FTO electrode. The potential enhancement mechanism was discussed, which lies in the contributions of CuClx(MBA)y in enhancing absorption in the visible-light region and boosting the separation of electron–hole pairs of TiO2 by the p–n junction. Furthermore, CuClx(MBA)y nanosheets can realize bioconjugation directly, thanks to its abundant carboxyl groups. The CuClx(MBA)y/TiO2/FTO composite photoelectrodes were applied to develop a sensitive PEC biosensor for microRNA-21 (model target). By subtly exploiting the energy transfer between CuClx(MBA)y and Au nanoparticles (AuNPs), AuNPs served as effective quenchers. In the presence of the target, AuNP-labeled sDNA1 connected to the electrode surface, and thus, a decreased photocurrent was obtained. The proposed biosensor has a low detection limit of 0.29 fM (S/N = 3), good selectivity, and reproducibility. The proposed system was applied to monitor microRNA in cancer cells with satisfying results.
研究领域:
生物化学
·
分析化学
·
纳米技术
·
分子生物学
·
癌症研究
✓ 已验证
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2020
·
IF 7.1
Sze Shin Low,Yixin Pan,Daizong Ji,Yaru Li,Yanli Lu,Yan He,Qingmei Chen,Qingjun Liu
Circulating microRNAs (miRNAs) are effective cancer biomarkers that are highly stable in body fluids enabling noninvasive detection. Portable, low-cost and efficient biosensing systems is crucial for early on-site diagnosis, especially in resource-limited settings. Present work deals with the development of a smartphone-based biosensing system for electrochemical detection of circulating microRNA-21 (miR-21) biomarker in saliva as a proof-of-concept. The smartphone-based biosensing system composed of a disposable screen-printed biosensor modified with reduced graphene oxide/gold (rGO/Au) composite, a circuit board for detection and a Bluetooth-enabled smartphone equipped with specially designed Android application. The hybridization between miR-21 target and ssDNA probe on the rGO/Au-modified electrode resulted in the decrease in peak current with increase in miR-21 target concentration. The smartphone-based biosensing system showed comparable performance with commercial electrochemical workstation, for miR-21 detection in the concentration range of 1 × 10 −4 M to 1 × 10 -12 M with correlation coefficient (r 2 ) of 0.99. In addition to good selectivity in discriminating mismatched and non-target sequences, the biosensing system also showed acceptable recovery rate ranging from 96.2%–107.2 % in spiked artificial saliva. The smartphone-based biosensing system is envisioned to be able to realize rapid and highly sensitive detection of circulating miRNA biomarker in body fluid for point-of-care testing in remote areas.
研究领域:
生物医学工程
·
生物传感器
·
即时诊断
·
癌症生物标志物
·
电化学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2014
·
IF 5.825
Mo Wang,Zhengliang Fu,Bingchen Li,Yunlei Zhou,Huanshun Yin,Shiyun Ai
Taking advantage of the special exodeoxyribonuclease activity of T7 exonuclease, a simple, sensitive, selective, and label-free microRNA biosensor based on the cyclic enzymatic amplification method (CEAM) has been proposed. First, thiol functionalized DNA probes were assembled onto a gold nanoparticles modified gold electrode surface through a Au–S bond, followed by hybridizing with target miRNA. Subsequently, DNA in RNA/DNA duplexes was digested by T7 exonuclease, which can release the microRNA molecules from the electrode surface and return into the buffer solution. Meanwhile, the released microRNA can further hybridize with the unhybridized DNA probes on the modified electrode surface. On the basis of it, an isothermal amplification cycle is realized. The T7 exonuclease-assisted CEAM achieved a low detection limit of 0.17 fM. Moreover, this assay presents excellent specificity with discriminating only a single-base mismatched microRNA sequence. Furthermore, this work can also be applied to detect avian leukemia based on the decreased expression level of microRNA-21.
研究领域:
生物化学
·
分子生物学
·
分析化学
·
癌症研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Bing Wang,Yu-Xiang Dong,Yu-Ling Wang,Jun-Tao Cao,Shu-Hui Ma,Yan-Ming Liu
A novel and simple photoelectrochemical (PEC) biosensing method for microRNA-21 (miRNA-21) detection is reported based on energy transfer (ET) between CdS:Mn doped structure (CdS:Mn) and Au nanoparticles (AuNPs). In this protocol, TiO 2 -CdS:Mn hybrid structure was used as a sensing platform for hairpin DNA immobilization. In the absence of miRNA-21, the immobilized DNA was in the hairpin form. In this state, the photocurrent of the electrode was greatly depressed, due to the effective ET effect produced by short interparticle distance between CdS:Mn and AuNPs. In the presence of miRNA-21, the hairpin DNA hybridized with miRNA-21 and changed into a more rigid, rodlike double helix, which forced the AuNPs away from the electrode surface, leading to obvious recover of photocurrent because of the vanished damping effect. Integrating the fine PEC performance of TiO 2 -CdS:Mn hybrid structure with the significant ET effect between CdS:Mn and AuNPs, the sensitive detection of miRNA-21 was realized in a linear range of 1.0 fM to 10.0 pM with a low detection limit of 0.5 fM. This method might be aussichtsreich for the detection of miRNAs and other biomarkers.
研究领域:
光电化学
·
生物传感器
·
纳米技术
·
分子生物学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
ACS Sustainable Chemistry & Engineering
·
2022
·
IF 9.224
Fu-Ting Wang,Jing Xu,Yang-Yang Hou,Ke-Jing Huang,Xin-Meng Yu,Xin Zhou,Xuecai Tan
A capacitor coupled with enzymatic biofuel cells (EBFCs) can deliver high-power instant output to obtain an amplified detection signal. Herein, matching a capacitor to a self-powered electrochemical biosensor for ultrasensitive determination of microRNA-21 is investigated. The detection system mainly combines a capacitor, EBFCs, and biological amplification technologies. Concretely, EBFCs are integrated into a capacitor-joined circuit, and the capacitor is automatically shorted by a switching regulator to provide an instantaneous current that is rapidly detected with a digital multimeter. A sensitivity of 38.72 μA/pM is achieved with the contribution of the matching capacitor, which is 6.96 times that without a capacitor. Moreover, the redox reaction on the anode leads to high-voltage outputs at low substrate concentrations and generates electrons when the target miRNA triggers a catalytic hairpin assembly cycle, while the release of the [Fe(CN)6]3– electron acceptor on the cathode is catalyzed to obtain a higher detection signal. As a result, the limit of detection of the developed biosensor is 0.18 fM (S/N = 3) with a linear range of 0.5–10 000 fM, which indicates that the innovative capacitor-matched self-powered biosensor holds great promise for ultrasensitive electrochemical biosensing.
研究领域:
生物化学
·
生物医学工程
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2021
·
IF 6.057
Yuanyuan Yao,Hongding Zhang,Tongtong Tian,Yixin Liu,Rendan Zhu,Ji Ji,Baohong Liu
With the emergence of microRNA (miRNA) as a key player in early clinical disease diagnosis, development of rapidly sensitive and quantitative miRNA detection methods are imperative. Herein, a label-free SERS assay coupled with duplex-specific nuclease (DSN) signal amplification strategy was proposed for facilely ultrasensitive and quantitative analysis of miRNA-21. Firstly, magnetic beads assembled with excessive capture DNA were utilized to hybridize the target miRNA-21. These DNA-RNA heteroduplexes were cleaved by DSN to generate small nucleotide fragments into the supernatant and the miRNA-21 released and rehybridized another DNA, going to the next DSN cycle. Consequently, numerous of small nucleotide fragments of capture DNA were released from magnetic beads and the miRNA-21 signal was transferred and amplified by the SERS signals of total phosphate backbones which are abundant in nucleotide. Furthermore, iodide-modified Ag nanoparticles (AgINPs) was employed to generate a strong and reproducible SERS signal. The proposed method displayed excellent performance for miRNA-21 detection with the linear range from 0.33 fM to 3.3 pM, and a lower detection limit of 42 aM. Moreover, this strategy exhibited effectively base discrimination capability and was successfully applied for monitoring the expression levels of miRNA-21 in different cancer cell lines and human serum.
研究领域:
生物传感器
·
分子诊断
·
分析化学
·
癌症研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2016
·
IF 7.476
Hong-Lei Shuai,Ke-Jing Huang,Ying-Xu Chen,Lin-Xia Fang,Meng-Pei Jia
An ultrasensitive electrochemical biosensor for detecting microRNAs is fabricated based on hollow molybdenum disulfide (MoS 2 ) microcubes. Duplex-specific nuclease , enzyme and electrochemical–chemical–chemical redox cycling are used for signal amplification . Hollow MoS 2 microcubes constructed by ultrathin nanosheets are synthesized by a facile template-assisted strategy and used as supporting substrate. For biosensor assembling, biotinylated ssDNA capture probes are first immobilized on Au nanoparticles (AuNPs)/MoS 2 modified electrode in order to combine with streptavidin-conjugated alkaline phosphatase (SA-ALP). When capture probes hybridize with miRNAs, duplex-specific nuclease cleaves the formative duplexes. At the moment, the biotin group strips from the electrode surface and SA-ALP is incapacitated to attach onto electrode. Then, ascorbic acids induce the electrochemical–chemical–chemical redox cycling to produce electrochemical response in the presence of ferrocene methanol and tris (2-carboxyethyl) phosphine. Under optimum conditions, the proposed biosensor shows a good linear relationship between the current variation and logarithm of the microRNAs concentration ranging from 0.1 fM to 0.1 pM with a detection limit of 0.086 fM (S/N=3). Furthermore, the biosensor is successfully applied to detect target miRNA-21 in human serum samples.
研究领域:
纳米技术
·
生物传感器
·
分子诊断
·
电化学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Qi Xiao,Jiawen Li,Xiaoyu Jin,Yi Liu,Shan Huang
An electrochemical biosensor for ultrasensitive detection of microRNA-21 (miRNA-21) coupling with carboxylate-reduced graphene oxide (COOH-rGO)-based signal-enhancing and duplex-specific nuclease (DSN)-assisted target recycling. Capture DNA (cDNA) was self-assembled on the surface of gold electrode (GE) and hybridized with the target miRNA-21 to form cDNA/miRNA-21 hybrids. COOH-rGO was further aggregated on cDNA/miRNA-21 hybrids through π − π stacking interaction, leading to a accumulation of electroactive methylene blue (MB) molecules and a significant differential pulse voltammetry (DPV) response of MB with peak potential of −0.28 V (vs. Ag/AgCl). At the present of DSN, DSN digested the cDNA/miRNA-21 hybrids and released miRNA-21 to hybridize with another cDNA, resulting in a significant decrease of the length of cDNA sequence. The amount of MB on the electrode surface decreased dramatically, following with the decrease of the DPV signal of MB. Under optimal experimental conditions, this approach displayed an ultrahigh sensitivity (with a 0.01 fM detection limit) and excellent selectivity among similar miRNAs sequences even with single-base difference. This biosensor further exhibited excellent applicability for target miRNA-21 in human serum samples with satisfactory results.
研究领域:
生物化学
·
分子生物学
·
纳米技术
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2021
·Vol. 188
·期 11
·1-11
·
IF 5.833
Wei Jinlan,He Sitian,Mao Yanhua,Wu Longjie,Liu Xinlian,Effah Clement Yaw,Guo Hongchao,Wu Yongjun
A simple nanoplatform based on molybdenum disulfide (MoS 2 ) nanosheets, a fluorescence quencher (signal off), and a hybridization chain reaction (HCR) signal amplification (signal on) used for the enzyme-free, label-free, and low-background signal quantification of microRNA-21 in plasma exosome is reported. According to the sequence of microRNA-21, carboxy-fluorescein (FAM)-labeled hybridization probe 1 (FAM-H1) and hybridization probes 2 (FAM-H2) were designed with excitation maxima at 488 nm and emission maxima at 518 nm. MoS 2 nanosheets could adsorb FAM-H1 and FAM-H2 and quenched their fluorescence signals to reduce the background signal. However, HCR was triggered when microRNA-21 was present. Consequently, HCR products containing a large number of FAM fluorophores can emit a strong fluorescence at 518 nm and could realize the detection of microRNA-21 as low as 6 pmol/L and had a wide linear relation of 0.01–25 nmol/L. This assay has the ability of single-base mismatch recognition and could identify microRNA-21 with high specificity. Most importantly, this approach was successfully applied to the detection of plasma exosomal microRNA-21 in patients with lung cancer, and it is proposed that other targets can also be detected by changing the FAM-H1 and FAM-H2 corresponding to the target sequence. Thus, a novel, hands-on strategy for liquid biopsy was proposed and has a potential application value in the early diagnosis of lung cancer. Graphical abstract
研究领域:
癌症诊断
·
分子生物学
·
纳米技术
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nanoscale
·
2022
·Vol. 14
·期 9
·3496-3503
·
IF 8.307
Shaohua Xu,Yueliang Wang,Yuanyuan Yao,Lifen Chen,Jiahui Xu,Bin Qiu,Longhua Guo
Highly sensitive detection of biomarkers is essential for disease prevention and early diagnosis. Herein, a highly sensitive strategy was proposed for microRNA-21 (miRNA-21) detection by the incorporation of programmable toehold-mediated strand displacement (TMSD) and dark-field microscopy imaging. Firstly, efficient and specific TSMD was carried out via hybridization between the substrate strand (Sub) and two short probe strands (P1, P2). Then, miRNA-21 could specifically hybridize with Sub due to the toehold that existed on its tail, which triggered the amplification with the help of the assist strands, and forming a large number of Sub-assist double-stranded DNA (dsDNA). This process realized the targeted highly specific recognition of miRNA-21 and the amplification of the trace target to high-output dsDNA. Additionally, as glucose oxidase (Gox) was modified on the end of the assist strands in advance, hydrogen peroxide was generated after adding glucose to the system, which further etched gold–silver core–shell nanocubes (Au@Ag NCs). As a result, the size of Au@Ag NCs decreased and the scattering intensity reduced simultaneously. The scattering intensity reduction value of Au@Ag NCs has a linear relationship with miRNA-21 concentration in the range of 1.0 to 100.0 fM with a limit of detection of 1.0 fM. Finally, the proposed method has been successfully demonstrated for the determination of miRNA-21 in lung cancer cell A549 lysate.
研究领域:
生物传感
·
纳米技术
·
分子生物学
·
癌症诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）