Antioxidants
·
2025
·Vol. 14
·期 8
·914
·
IF 6.6
Pengxin Jiang,Kangping Liu,Yanan Cui,Puyu Liu,Xutao Wang,Zijuan Hou,Jiamei Cui,Ning Chen,Jinghui Fan,Jianguo Li,Yuzhu Zuo,Yan Li
EnterotoxigenicEscherichia coli(ETEC) leads to severe diarrhea in piglets. Naringenin (Nar), a natural flavonoid compound, is known for its antibacterial and anti-antioxidant properties. However, the protective effects of Nar against ETEC-induced diarrhea have not been reported yet. This study investigated the protective mechanisms of Nar against ETEC infection in porcine intestinal epithelial cells (IPEC-J2). ETEC infection induced oxidative stress and ferroptosis in IPEC-J2 cells by elevating intracellular iron content and ROS accumulation, increasing MDA levels, downregulating SOD activity and GPX4 expression, and upregulating the transcription of CHAC1 and SLC7A11. In contrast, Nar suppressed ETEC-induced ferroptosis of IPEC-J2 cells by inhibiting the SLC7A11/GPX4 pathway. Specifically, Nar mitigated mitochondrial damage, reduced intracellular iron levels and ROS accumulation, and ultimately reversed the oxidative stress. Network pharmacology and molecular docking identified heat-shock protein 90 (HSP90) as a potential target of Nar. Overexpression and knockdown experiments revealed that ETEC-induced ferroptosis was mediated by upregulation of HSP90, while the protective effects of Nar against ETEC-induced ferroptosis were dependent on the downregulation of HSP90. In conclusion, Nar targets host HSP90 to protect IPEC-J2 cells from ferroptosis caused by ETEC infection. This study demonstrates that Nar is a potent antioxidant natural compound with potential for preventing ETEC-induced intestinal damage.
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SEPARATION AND PURIFICATION TECHNOLOGY
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2025
·
IF 9
Chao Wang,Peijuan Chu,Tengfei He,Cheng Yi,Yue Yao,Lijia Liu,Chunhong Zhang
The application of membrane separation technology in wastewater treatment and pharmaceutical purification has attracted considerable attention. The development of a membrane with effective separation performance and antibacterial properties is of considerable interest for the separation and purification of antibiotics. Here, a thin-film composite (TFC) polyester membrane with effective antibiotic removal and antibacterial properties was fabricated via a facile one-step interfacial polymerization (IP) strategy. Naringenin (Nar), a natural product of flavonoid material derived from the pericarp, was utilized as the aqueous monomer. The suitable reactivity of Nar afforded the resulting membrane with a highly negative charged surface. The rejection performances for the antibiotics cephalothin, cefuroxime sodium, ceftriaxone sodium, and cefoperazone sodium were 96.3 %, 97.7 %, 99.2 %, and 99.8 %, respectively. The pure water permeance was 7.5 L m −2 h −1 bar −1 at a transmembrane pressure of 5 bar. The antibacterial efficacy was greater than 99.0 % against Escherichia coli and Staphylococcus aureus , attributable to the flavonoid structure of Nar. The present work proposed a feasible strategy to prepare TFC membranes with high nanofiltration performance and intrinsic antibacterial efficacy.
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BIOFACTORS
·
2021
·Vol. 48
·期 1
·190-203
·
IF 6.113
Huijie Chen,Jianqing Chen,Xu Shi,Lu Li,Shiwen Xu
Bisphenol A (BPA) has caused serious pathologies in varying organs of humans and animals, especially reproductive organs. Naringenin (NRG) is a flavanone compound that has shown protective effects against several environmental chemicals through suppression of oxidative stress and activation of nuclear factor erythroid 2-related factor 2 (Nrf2) pathway. Herein, we described the discovery path of NRG inhibition on apoptosis in BPA exposed swine testis (ST) cells through targeting Kelch-like ech-associated protein (Keap1). We found that NRG could specifically bound to the active residues of DGR domain in Keap1, thereby activating Nrf2 signaling pathway, and then increasing the levels of SOD, GPx and CAT, and finally inhibiting oxidative stress and mitochondrial apoptosis induced by BPA in ST cells. Altogether, our results showed that NRG inhibits oxidative stress and mitochondrial apoptosis induced by BPA in ST cells by targeting Keap1/Nrf2 signaling pathway, indicating that NRG could serve as an antagonistic therapy against BPA.
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JOURNAL OF DRUG TARGETING
·
2023
·
IF 4.5
Chang Yan,Jinlian Gu,Shun Yin,Hao Wu,Xia Lei,Fang Geng,Ning Zhang,Xiaodan Wu
Efficient brain drug delivery has been a challenge in the treatment of Alzheimer’s Disease and other brain disorders as blood-brain barrier (BBB) impedes most drugs to reach brain. To overcome this obstacle, we developed a novel TGN decorated erythrocyte membrane-coated poly (lactic-co-glycolic acid) nanoparticle (TRNNs). The nanoparticle significantly boosted the penetration (7.3 times) in a U-118MG and HCMEC/D3 cell co-culture BBB model in vitro. Living image was performed to assess the TRNNs distribution in vivo. The fluorescence intensity in the isolated brain of TRDNs-treated mice was about 8 times that of the DNs-treated. In the novel object recognition test, the mice after administration of TRDNs showed higher recognition index (0.414 ± 0.016) than the model group (0.275 ± 0.019). A significant increase in the number of dendritic spines from TRNNs administrated mice hippocampi neurons was observed after Golgi stain. This improvement of neurons was also confirmed by the significant high expression of PSD95 protein level in hippocampi. We measured the OD values of Aβ25-35 induced PC12 cells that pre-treatment with different nanoparticles and concluded that TRNNs had a robust neuroprotection effect. Above all, functional biomimetic nanoparticles could increase the accumulation of naringenin into brain, thereby enable the drug to exert greater therapeutic effects.
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FOOD HYDROCOLLOIDS
·
2020
·
IF 7.053
Xin Yin,Xiaojun Fu,Hao Cheng,Wusigale,Li Liang
Food proteins are being used as carrier materials for the co-encapsulation of bioactive components. Partition of bioactive components in a carrier depends on their solubility and interaction with carrier materials. In this study, hydrophobic α-tocopherol and amphiphilic naringenin were chosen as model bioactive components to construct their complex nanoparticles with whey protein isolate (WPI). Naringenin in the molecular level was bound to WPI particles, while α-tocopherol was both bound in the molecular level and encapsulated as the aggregate in the particles. In ternary complex particles, the increased antioxidant activity of WPI-naringenin particles counteracted the protein shielding effect on the activity of α-tocopherol. Oxidation of α-tocopherol and naringenin did not cause any damage to WPI, but slightly changed the size and ζ-potential of protein particles during storage. Addition of naringenin improved the stability and bioaccessibility of α-tocopherol. The data gathered here would provide guidance for the development of protein-based co-encapsulating carriers.
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FOOD CHEMISTRY
·
2025
·
IF 9.8
Juanqiong Ma,Na Yang,Xingyuan Zhang,Manyi Xiao,Cheng Cao,Zhiyong Xie,Pei Li,Qiongfeng Liao,Yanlong Chen
Naringenin is a plant flavonoid found in citrus fruits and is known for its anti-inflammatory and antioxidant activities. However, extracting high-purity naringenin from citrus fruits and separating its enantiomers remains challenging. This study synthesized a molecularly imprinted multiple-component covalent organic framework (MI COF) with abundant imprinted recognition sites for the first time. This MI COF combines the advantages of molecular imprinting and covalent organic frameworks into an innovative adsorbent capable of specifically recognizing and efficiently adsorbing naringenin. MI COF exhibited an excellent adsorption capacity of 121 mg/g and a superior selectivity with an imprinting factor of 2.8 for naringenin. As a result, remarkably selective extraction of naringenin from citrus fruit extracts was achieved, with recoveries and purities of 85.7 % and 87.5 %, respectively. In addition, the vitro immunological and anticancer activities of naringenin enantiomers from citrus fruit was preliminarily investigated. These findings demonstrate that MI COF is an effective sorbent for the selective adsorption and separation of naringenin and its enantiomers from plants and fruits, holding significant potential for practical applications in food and clinical fields.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Hong Feng,Ting Li,Lian Zhou,Lei Chen,Qingyun Lyu,Gang Liu,Xuedong Wang,Xi Chen
In this study, potato starch (PS)/naringenin (NAR) complex was prepared, and its properties and emulsification behavior were evaluated. The experimental results demonstrated that NAR successfully formed a complex with PS molecules through hydrogen bonds and other non-covalent interactions. The emulsifying capacity (ROV) of PS/NAR complex with 16 % composite ratio was 0.9999, which was higher than PS (ROV = 0.3329) ( p < 0.05). Based on particle property analysis and molecular dynamics simulation, the mechanism of improving the emulsification performance might be the action of the benzene ring of NAR and intermolecular hydrogen bonding. In addition, the stability of the Pickering emulsions with PS/NAR complexes as emulgators was significantly improved. The emulsifying and rheological behavior of starch-based Pickering emulsions could be adjusted by changing the proportion of the complexes. Results demonstrated that the PS/NAR complexes might be a prospective stabilizer of Pickering emulsions based on starch material and might expand the use of PS in edible products.
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JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
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2026
·
IF 4
Ming Wu,Yu Liang,Hao Lei,Lin Li,Xia Zhang,Yi Yuan,Bing Li
BACKGROUND Due to weak stability and bioavailability, γ -aminobutyric acid (GABA) and naringenin (Nar), two agents with sleep-promoting activities, are limited in practical application. To improve their sleep-promoting efficacy, this study developed a stability-enhanced co-loaded liposomal system (GABA-Nar-Lip) as a lyophilized powder. RESULTS The non-lyophilized GABA-Nar-Lip exhibited good dispersibility, with an average particle size of 161.34 ± 1.68 nm, and achieved encapsulation efficiencies of 46.18 ± 0.32% (GABA) and 82.01 ± 1.33% (Nar), respectively. The stability of GABA-Nar-Lip was significantly enhanced after lyophilization with sugars (especially 0.10 g mL −1 trehalose) as cryoprotectants (CPAs), and maintained targeted release in the small intestine. The CPAs exerted cryoprotective effects by replacing water molecules and forming hydrogen bonds with soy phosphatidylcholine, thereby preserving the spherical structure and unilamellar vesicle morphology of the liposomes. In caffeine-induced zebrafish models, lyophilized GABA-Nar-Lip showed an inferior but still significant effect in sleep latency reduction. Moreover, it decreased nocturnal twitching activity and enhanced daytime locomotor activity on the following day, without the adverse effects associated with melatonin, such as poor sleep quality and reduced daytime activity. CONCLUSION The stabilized lyophilized GABA-Nar-Lip powder is a promising, safe sleep aid with balanced efficacy and no compromise to next-day performance, supporting its potential application in dietary sleep interventions. © 2026 Society of Chemical Industry.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Meng Ding,Yuan Zhu,Xiaoting Xu,Hui He,Tianyu Jiang,Xiaochuan Mo,Zhuting Wang,Wenfeng Yu,Hailong Ou
Macrophage inflammation and oxidative stress promote atherosclerosis progression. Naringenin is a naturally occurring flavonoid with antiatherosclerotic properties. Here, we elucidated the effects of naringenin on monocyte/macrophage endothelial infiltration and vascular inflammation. We found naringenin inhibited oxidized low-density lipoprotein (oxLDL)-induced pro-inflammatory cytokines such as IL-1β, IL-6, and TNF-α toward an M2 macrophage phenotype and inhibited oxLDL-induced TLR4 (Toll-like receptor 4) membrane translocation and downstream NF-κB transcriptional activity. Results from flow cytometric analysis showed that naringenin reduced monocyte/macrophage infiltration in the aorta of high-fat-diet-treated ApoE-deficient mice. The aortic cytokine levels were also inhibited in naringenin-treated mice. Further, we found that naringenin reduced lipid raft clustering and acid sphingomyelinase (ASMase) membrane gathering and inhibited the TLR4 and NADPH oxidase subunit p47phox membrane recruitment, which reduced the inflammatory response. Recombinant ASMase treatment or overexpression of ASMase abolished the naringenin function and activated macrophage and vascular inflammation. We conclude that naringenin inhibits ASMase-mediated lipid raft redox signaling to attenuate macrophage activation and vascular inflammation.
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FOOD CHEMISTRY
·
2025
·
IF 9.8
Simin Feng,Shuqi Liang,Jianyu Yang,Jiahao Yu,Yang Lin,Yizhen Xie,Peilong Sun,Ping Shao
This study developed a synergistic lipid-lowering system using chitin-glucan complex (CGC) from Flammulina velutipes through green preparation and structural optimization. Enzyme-alkali treated CGC3 exhibited superior acetylation (47.85 %), crystallinity (42.29 %), and molecular weight (1.848 × 10 6 Da) through partial glucan removal. The derived hydrogel (gel-CGC3) had a dense porous network (density 2.92 ± 0.09 × 10 −3 g/cm 3 ), excellent mechanical stability, and regular pore structure. Ball milling prolonged naringenin (NAR) release to 900 min (87.5 % longer than block hydrogels) without compromising structural integrity. Meanwhile, the microgel conferred multiple functional advantages to the system (includes CGC gel and NAR loaded), and its porous structuring (powder-to-hydrogel transition) and structural refinement ( via microgel conversion) maximized binding site exposure, enhancing adsorption efficiency for cholesterol micelles (12.42 ± 0.73 mg/g), sodium taurocholate (3.91 ± 0.07 mg/g), and sodium glycopyrrolate (3.51 ± 0.07 mg/g) by exposing more binding sites. The system synergistically combines lipid adsorption and biometabolism, underscoring the significance of structure-function synergy in polysaccharide-based platforms for metabolic disorder intervention.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Anyi Wu,Junping Zhou,Yinan Xue,Yun Liu,Jidong Shen,Xin Gao,Zhiqiang Liu,Yuguo Zheng
Icaritin (ICT) shows various pharmacological activities and is used as an anti-hepatocellular carcinoma drug. While most of the studies focused on constructing yeast chassis cells for ICT production to replace the ICT extraction of Epimedium species, an efficient multi-enzyme system was first constructed here in the prokaryote Escherichia coli , for the successful biotransformation from naringenin (NAR) and prenol to ICT. The plant-derived flavonoid 3-hydroxylase (F3H) and flavonol synthase (FLS) were screened, and then fused together with protein linkers to efficiently catalyze the conversion of NAR to kaempferol (KAE). The isopentenol utilization (IU) pathway was subsequently introduced to increase dimethylallyl diphosphate (DMAPP) supply, along with catalytic activity improvement of prenyltransferase (PT) using N -terminal truncations and active site pocket engineering. Furthermore, by screening O -methytransferases (OMT) from different sources, EpOMT 4 from Epimedium pseudowushanense was selected for the ICT production. With the combination of these strategies, 80.8 ± 3.6 mg/L ICT could be produced from NAR and prenol in the recombinant E. coli , from the original 0 % degree of conversion. The systematic engineering of multi-enzyme system for ICT biosynthesis has laid the foundation for the large-scale ICT production using the industrially important E. coli chassis cell.
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CURRENT PHARMACEUTICAL DESIGN
·
2025
·Vol. 31
·期 25
·2025-2037
·
IF 2.8
Linhong Jiang,Dandan Bai,Yunjun Yang,Yingying Wu,Yanran Chen,Mengyuan Wang,Wenxin Wang,Haixia Wang,Yanzhe Xiong,Siqi Zhu,Xinyu He,Jun Long,Dongping Yuan,Jingwei Chen
ObjectiveNaringenin (NAR) is a naturally occurring tiny molecule that has a significant role in lipid metabolism. However, the molecular mechanism by which NAR is involved in lipid metabolism to protect ECs is not clear. This study aims to investigate the effect of NAR on autophagy in oxidized low-density lipoprotein (ox-LDL)-treated human umbilical vein endothelial cells (HUVECs) and its potential molecular mechanisms.MethodsOxidized LDL-induced HUVECs injury in vitro was treated with NAR. Chloroquine was used as an autophagy inhibitor. Ionomycin (Iono) and 2-aminoethoxydiphenylborate (2-APB) were used as an SOCE pathway agonist and an inhibitor, respectively. The autophagy levels in HUVECs were determined by quantitative real-time PCR, Western blot, and immunofluorescence methods. The concentration of calcium ions in HUVECs was measured by flow cytometry.ResultsThe findings revealed that NAR increased the viability of ox-LDL-impaired HUVECs. NAR increased the level of autophagy and decreased lipid accumulation in ox-LDL-treated HUVECs, which could interfere with chloroquine. Moreover, NAR significantly downregulated the expression of STIM1 and ORAI1 proteins and Ca2+ levels in the SOCE, which could be interfered with Iono or 2-APB, respectively.ConclusionIn summary, NAR can increase autophagy levels and decrease lipid accumulation in HUVECs, eventually protecting against ox-LDL-induced injury in HUVECs, which is associated with inhibition of the SOCE pathway.
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