ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2025
·
IF 6.1
Siqi Yu,Fudong Wang,Yuan Zhang,Xiaofeng Wang,Hongyu Zhao,Qiang Zheng
Autophagy inhibition induced by paraquat (PQ) poisoning is an important factor causing multi-organ damage, including renal tissue. Dihydrocapsaicin (DHC), the main active ingredient in chili peppers, was reported to induce autophagy, but its effect on PQ-induced AKI remains unclear. Rats were intraperitoneally injected with 25 mg/kg PQ to establish AKI rat model followed immediately by DHC administration (2.5, 5, or 10 mg/kg, i.p.). Rat renal tubular epithelial cells were exposed to PQ (300 μM) in combination with DHC (25, 50, or 100 μM) for 24 h. The results showed that DHC treatment attenuated PQ-induced renal injury and dysfunction while suppressing apoptosis and oxidative stress in vivo and in vitro . Furthermore, DHC administration induced autophagy—a protective mechanism against oxidative stress and apoptosis—as demonstrated by: 1) increased autophagosome/autophagolysosome formation, 2) upregulated LC3-II and autophagy-related gene 7 expressions and reduced p62 protein level. Blocking of autophagy by 3-methyladenine (3-MA) reversed the effects of DHC. Interestingly, transcription factor sex determining region Y-box 9 (Sox9) and autophagy inducer Sestrin2 RNA levels were increased by DHC treatment. Sox9 activated Sestrin2 transcription through direct binding to its promoter region. Sox9 or Sestrin2 silencing reversed the effects of DHC. Collectively, our findings demonstrate that DHC attenuates PQ-induced AKI via Sox9/Sestrin2 axis-mediated autophagy, indicating therapeutic utility of DHC against AKI.
研究领域:
药理学
·
毒理学
·
分子生物学
·
肾脏病学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Cleaner Production
·
2025
·
IF 10
Chaonan Li,Yiping Liu,Wenfei Liao,Yalan Mo,Xiaogang Li
Paraquat, an effective non-selective herbicide, has been widely utilized in agriculture, posing significant risks of human poisoning. In this study, paraquat is encapsulated within amino-functionalized metal-organic frameworks (UiO-66-NH 2 ), with cinnamic acid derivatives employed as “gatekeepers” and microparticles coated through host-guest interactions with β-cyclodextrin to regulate paraquat release, forming a dual-coated photoresponsive controlled-release system (PQ@MOF@CC@CD). The synthesized PQ@MOF@CC@CD microparticles exhibit an octahedral morphology, with an average particle size of 457.37 nm and a paraquat loading capacity of 22.07 %. Upon exposure to UV/sunlight irradiation, cinnamic acid derivatives on the microparticle surface undergo trans-cis isomerization, leading to their dissociation from β-CD cavities. This process induces coating shedding, pore opening, and paraquat release. In vitro release studies confirm the microparticles’ strong photoresponsiveness and sustained-release properties. Furthermore, these microparticles effectively prevent paraquat degradation in alkaline environments. Herbicidal activity assessments indicate that PQ@MOF@CC@CD exhibits comparable efficacy to free paraquat at equivalent doses under sunlight. Compared to paraquat technical formulations, PQ@MOF@CC@CD microparticles demonstrate improved safety in cellular models and murine assays. Overall, PQ@MOF@CC@CD represents a cleaner agrochemical strategy, offering a dual benefit of minimizing human exposure risks and environmental impact through controlled pesticide release and safeguarding agricultural productivity, thereby advancing sustainable herbicide management practices.
研究领域:
农业化学
·
材料科学
·
环境科学
·
纳米技术
·
可持续农业
·
农药科学
✓ 已验证
产品关联
DOI
高影响因子
CLAY MINERALS
·
2024
·
IF 1.1
Rui Liu,Chao Ma,Huanliang Chen,Changsuo Li,Lei Zuo,Peng Zhang,Yaqing Wang,Ru Wang
Clay minerals are effective adsorbents for the remediation of pesticides in wastewater due to their large superficial areas and excellent cation-exchange capabilities. However, this adsorption effect can be reduced by the accumulation of adsorbents on clay minerals, amongst other problems. Therefore, in this study, montmorillonite (Mnt) modified by layered double hydroxide (LDH) with different loading amounts was successfully prepared using an in situ method. The results from X-ray diffraction, Fourier-transform infrared spectrometry, Brunauer–Emmet–Teller (BET) and scanning electron microscopy analyses revealed that LDH structures were successfully combined with the Mnt layer and formed a porous structure. However, excess LDH still caused the aggregation and accumulation of layers. The adsorption performance of LDH@Mnt for atrazine (ATZ) and paraquat (PQ) was investigated, and the removal efficiency of the LDH@Mnt composite was higher than those of Mnt and LDH alone. The kinetic study revealed that the adsorption process fitted the pseudo-second-order model and internal diffusion model, and 3-LDH@Mnt had the greatest absorbability efficiency for both ATZ and PQ, indicating the adsorption process was controlled by the number of active sites of the adsorbent. The generalized Langmuir model accurately characterized the adsorption process of ATZ and PQ elimination in the adsorption isotherm investigation, indicating that the adsorption energies of the active sites on the adsorbents were different. 3-LDH@Mnt had better absorbability performance for ATZ/PQ, and the sorption capacities were 7.03 and 91.9 mg g–1, respectively. According to site energy distribution theory, the amount of sorption sites of the composite adsorbent was large and the average adsorption energy was high, both of which being beneficial for the adsorption of ATZ and PQ. The effects of pH, coexisting anions and reuse experiments were also tested, indicating that the LDH@Mnt composite possessed high adsorption stability. This excellent removal performance represents a promising strategy for the remediation and elimination of pesticide contaminations from the environment.
研究领域:
环境科学
·
土壤学
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Nianlu Li,Mengping Zhang,Xiao Geng,Ranran Liu,Xiao Meng,Wei Zou,Wenwen Chen,Hua Shao,Cuijuan Wang
Poisoning with diquat (DQ) and paraquat (PQ) herbicides has been a public health problem. Unfortunately, rapid detection in biofluids remains challenging. Herein, a hyphenated approach of electro-enhanced adsorption, solid-phase microextraction, and surface-enhanced Raman spectroscopy (EE−SPME−SERS) was exploited for rapid and sensitive in situ analysis of DQ and PQ in human urine and plasma. A porous Ag fiber with an Au layer (p-Ag/Au fiber) was fabricated with multi-functions (SERS substrate, SPME sorbent , and working electrode). Clear trends could be observed between signal intensity and concentration of DQ or PQ with high reproducibility. The method detection limits (MDLs) calculated from the characteristic peak of DQ were 0.064 ppb and 0.65 ppb in urine and plasma, respectively. Corresponding MDLs for DQ and PQ in urine and plasma were 0.036 ppb and 0.35 ppb, respectively. The whole process of “extracting and detecting” was completed within 4 min with satisfactory recoveries. Notably, the EE−SPME−SERS technique could easily realize in situ analysis of DQ and PQ in biofluids with good selectivity, which is practical in clinical diagnosis. This EE−SPME−SERS method provides a potential avenue for efficient pretreatment and in situ poison detection in biological samples.
研究领域:
分析化学
·
毒理学
·
临床医学
✓ 已验证
产品关联
DOI
高影响因子
Oxidative Medicine and Cellular Longevity
·
2022
·
IF 7.31
Qiang Zheng,Yuan Zhang,Xiaofeng Wang,Fudong Wang,Hongyu Zhao
Paraquat (PQ) is a widely used herbicide but can be lethal to humans. The kidney is vital for PQ elimination; therefore, explorations for therapeutic approaches for PQ-induced acute kidney injury (AKI) are of great significance. Here, the effects of a natural bioactive polyphenol isorhapontigenin (ISO) on PQ-AKI were investigated. In vitro experiments carried out in PQ-intoxicated rat renal tubular epithelial cells (NRK-52E) showed that ISO treatment inhibited PQ-induced cell apoptosis and oxidative stress, which was evidenced by the decreased proapoptotic proteins [cleaved caspase 3/9 and poly (ADP-ribose) polymerase (PARP)], the reduced oxidative stress indicators [reactive oxygen species (ROS), malondialdehyde (MDA), and lactate dehydrogenase (LDH) leakage], and the increased antioxidants [superoxide dismutase (SOD), nuclear factor E2-related factor 2 (NRF2), and oxygenase-1 (HO-1)]. Furthermore, 50 mg/kg ISO pretreatment before PQ administration significantly attenuated PQ-AKI in rats, as manifested by the improved renal tubule damage, the reduced serum and urine markers of kidney injury, and the inhibited cell apoptosis and oxidative stress in the renal cortex. Furthermore, expression of sex-determining region Y box 9 (SOX9) and Toll-interacting protein (TOLLIP) in NRK-52E cells and the renal cortex was significantly upregulated after ISO treatment. Overexpression of SOX9 increased TOLLIP transcription and attenuated PQ-induced apoptosis and oxidative stress, whereas knockdown of SOX9 impaired the protective effects of ISO on NRK-52E cells against PQ toxicity. In conclusion, the present study demonstrated that ISO modulated SOX9/TOLLIP expression to attenuate cell apoptosis and oxidative stress in PQ-AKI, suggesting the potential of ISO in treating PQ-poisoned patients.
研究领域:
药理学
·
毒理学
·
肾脏生理学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMISTRY-A EUROPEAN JOURNAL
·
2026
·
IF 3.6
Shengke Li,Yiyun Gao,Miao Cheng,Hao Zhou,Kehan Zhang,Ruibing Wang,Aihua Zou
Controllable and on-demand delivery of nanopesticides have great potential to enhance pesticide utilization and facilitate sustainable agriculture. Photo-responsive supramolecular host–guest systems capped on mesoporous nanomaterials are deemed as competitive nanovalves for efficient and straightforward control of pesticide release. Herein, azobenzene-modified mesoporous silica nanoparticles (Azo-MSNs) were fabricated for loading the pesticide imidacloprid (IMI) and subsequent construction of photo-responsive nanopesticides by taking advantage of the ternary host–guest complexes of cucurbit[8]uril(CB[8])/ trans -Azo/paraquat (PQ) as nanovalves. UV-irradiation will open the nanovalves due to attenuated binding affinity of CB[8]/PQ/ trans -Azo complexes, thus triggering the release of trapped IMI inside Azo-MSNs. In detail, MSNs-as (∼160 nm) were synthesized with a template agent and directly used to produce surface-modified Azo-MSNs-as. After template removal, the obtained Azo-MSNs were loaded with IMI to produce IMI@Azo-MSNs (35.60% pesticide loading), which were treated with CB[8]/PQ complexes to construct photo-responsive supramolecular nanopesticides IMI@CB[8]/PQ/Azo-MSNs. The supramolecular nanopesticides can achieve controllable and on-demand release of IMI upon 6 h of UV irradiation (total 51.20% vs. 87.95% during 11 h). This work offers an efficient and straightforward approach to constructing photo-responsive nanopesticides and holds significant importance for the sustainable development of modern agriculture. Graphical Cucurbit[8]uril-based ternary complexes with paraquat and azobenzene are employed as photo-responsive nanovalves for the facile and efficient construction of smart nanopesticides, enabling controllable and on-demand pesticide release. This strategy holds great promise for promoting the sustainable development of modern agriculture.
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2024
·
IF 12.2
Chenghu Yuan,Cui Tang,Xuejia Zhan,Menglin Zhou,Lei Zhang,Wen-Tong Chen,Abdukader Abdukayum,Guangzhi Hu
Paraquat (PQ) is a widely used and harmful herbicide that must be detected in the environment. This study reports a novel composite (CoS 2 -GCN) prepared by assembling cobalt disulfide (CoS 2 ) derived from metal-organic frameworks (MOFs) on graphitic carbon nitride (GCN). An electrochemical sensor (CoS 2 -GCN/ glassy carbon electrode (GCE)) was successfully prepared by modifying CoS 2 -GCN onto a GCE to sensitively detect PQ. Different concentrations of PQ were detected using square-wave voltammetry, and the CoS 2 -GCN/GCE electrochemical sensor showed remarkable response signals for PQ in the range of 20 – 1000 nM and 1 – 13 μM, with a detection limit of 4.13 nM (S/N = 3). The CoS 2 -GCN/GCE electrochemical sensor exhibited high stability, reproducibility, and immunity to interference, which were attributed to the synergistic effects of CoS 2 and GCN. In addition, the CoS 2 -GCN/GCE electrochemical sensor showed high applicability for the analysis of fruit samples. Therefore, the proposed sensor has potential applications in PQ detection.
研究领域:
环境科学
·
分析化学
·
材料科学
·
农业化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Water Process Engineering
·
2025
·
IF 6.7
Liling Zeng,Xianyan Ao,Manli Xu,Yunqian Zhang,Zhu Tao
The synergistic removal of organic pollutants by adsorption and photocatalysis can be achieved by adsorption of enriched pollutants, followed by photocatalytic degradation to decompose the pollutants into non-toxic substances. In this work, a category of adsorption-photocatalytic bifunctional composite materials Q[6]-HPAs have been developed through the self-assembly of cucurbit[6]uril (Q[6]) and heteropoly acids (HPAs) like phosphomolybdic acid (PMA), phosphotungstic acid (PTA) and silicotungstic acid (STA). These composites exhibit enhanced surface area, structural stability, and extended light absorption into the visible range. Notably, Q[6]-HPAs have surface charge properties that attract cations and an energy band structure suitable for the generation of reactive oxygen species, thus possessing the potential for efficient adsorption and photodegradation of cationic pesticides. Among which, Q[6]-PMA and Q[6]-PTA removed over 95 % of diquat and paraquat through sequential adsorption and photodegradation. Electrostatic attraction drives the adsorption process, and ·O 2 − and ·OH radicals detected via Electron Spin Resonance (ESR) are the key to photodegradation. Ultimately, Liquid Chromatography-Mass Spectrometer (LC-MS) analysis revealed potential degradation intermediates and products, and toxicity analysis was performed.
研究领域:
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Huixin Zhou,Yuzhen Wang,Liting Xu,Ying Zhou,Yanping Hong,Dan Hou,Xiaoning Liao
Electrochemical sensor has gained ever-increasing attention in screening residues in food samples for its rapid response and portability, which, however, is strongly obstructed by the poor stability and fouling effect derived from electrode materials. Herein, a novel polymer-based nanocomposite, namely Nafion co-modified with highly conductive graphene and multiwalled carbon nanotubes (Nf-Gr-MWCNTs-COOH), was developed for paraquat (PQ) detection via a facile sonication assisted preparation strategy. Electrochemical and physical characterizations together with theory calculation revealed that the conductivity and surface area of Nafion which has specific redox response to PQ were greatly enhanced by Gr-MWCNTs-COOH. Meanwhile, Nafion increased the dispersion and stability of Gr-MWCNTs-COOH. Therefore, the Nafion-based nanocomposite presented excellent sensitivity and stable output signal in PQ detection after coating on the glass carbon electrode. Under optimized conditions, the sensor displayed a wide linearity (0.01 – 15 μmol·L -1 ) and a very low limit of detection (0.005 μmol·L -1 ). Additionally, the sensor showed excellent repeatability, reproducibility and long-term stability thanks to the inherent high stability and antifouling properties of Nf-Gr-MWCNTs-COOH. The practical application of the developed sensor was evaluated by detection of PQ in real food samples including black tea, lettuce, and corn. Satisfactory recoveries, ranging from 95.00 % to 101.7 % with RSDs from 0.30 % to 3.0 %, were obtained. The high accuracy and excellent stability of the proposed sensor suggest its highly promising in commercial use.
研究领域:
分析化学
·
食品科学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
Molecular Medicine Reports
·
2020
·Vol. 22
·期 5
·3687-3694
·
IF 2.1
Yingli Ren,Xiangdong Jian,Zhongchen Zhang,Qiong Ning,Baotian Kan,Li Kong
Paraquat is a highly toxic pesticide, which often causes pulmonary interstitial fibrosis after poisoning, and there is no specific antidote. At present, limited studies have reported that tacrolimus, as an immunosuppressant, can inhibit pulmonary fibrosis, but the specific mechanism remains unknown. The aim of the present study was to demonstrate the effect of tacrolimus on the TGF‑β1 pathway associated with pulmonary fibrosis in paraquat exposed alveolar type II epithelial cells, and to identify the antipulmonary fibrosis mechanism of tacrolimus The rat alveolar epithelial type II RLE‑6TN cell line was exposed to paraquat and treated with or without tacrolimus for 24 h, or with a TGF‑β1 receptor type I/II inhibitor (LY2109761) for 1, 4, 8 or 16 h. MTT assays were used to detect the viability of rat alveolar type II epithelial cells under these different treatment conditions, while the concentrations of TGF‑β1, SMAD3, SMAD7 and connective tissue growth factor (CTGF) in the cell culture supernatant were determined using ELISAs. Additionally, reverse transcription‑quantitative PCR and immunofluorescence were used to analyze the mRNA and protein expression levels of TGF‑β1, SMAD3, CTGF and SMAD7. The results demonstrated that the inhibition of the proliferation of RLE‑6TN cells exposed to 200 nmol/l paraquat was 26.05±2.99%. The inhibition rate of 10 ng/ml tacrolimus on paraquat‑exposed alveolar type II epithelial cells was 18.40±3.49%. The inhibition rate caused by 5 µmol/l LY2109761 was 26.56±4.49%. The expression levels of TGF‑β1, SMAD3 and CTGF, as well as their concentrations in the culture supernatant, were significantly downregulated in the tacrolimus group compared with the paraquat group. However, both the concentration and expression levels of SMAD7 were significantly upregulated in the tacrolimus group compared with the paraquat group. In conclusion, tacrolimus can reduce the levels of TGF‑β1, SMAD3 and CTGF, increase the level of SMAD7 in TGF‑β1 signaling pathway and protect the development of pulmonary fibrosis in paraquat exposed alveolar epithelial cells.
研究领域:
药理学
·
肺病学
·
细胞生物学
·
毒理学
✓ 已验证
DOI
PubMed
高影响因子
TOXICOLOGY AND APPLIED PHARMACOLOGY
·
2021
·
IF 4.219
Qiang Zheng,Yuan Zhang,Zheng Zhao,Haitao Shen,Hongyu Zhao,Min Zhao
Isorhynchophylline (IRN) is an alkaloid with anti-inflammatory and anti-oxidative activities in cardiovascular and brain diseases, but its role in paraquat (PQ)-induced acute kidney injury (AKI) is yet unknown. The model of PQ-induced AKI in rats was established by intraperitoneal injection of PQ (25 mg/kg). We found that the tail vein injection of IRN (4 mg/kg) significantly increased the survival rate of PQ-intoxicated rats. IRN administration alleviated PQ-induced renal injury and renal dysfunction in rats, as evidenced by decreased apoptosis in renal cortex and reduced serum creatinine, serum BUN , and urine NGAL levels. Furthermore, IRN treatment improved the PQ-triggered oxidative stress in renal cortex by increasing the levels of anti-oxidant indicators (SOD activity, GSH/GSSG ratio, levels of Nrf-2, NQO-1, and HO-1 in renal cortex) and decreasing the levels of oxidative stress indexes (ROS and MDA levels in renal cortex). Interestingly, toll-interacting protein (Tollip), a negative regulator of interleukin 1 receptor associated kinase 1 (IRAK1) phosphorylation, was demonstrated to be increased by IRN injection in the renal cortex of PQ-intoxicated rats. In vitro experiments revealed that IRN protected renal tubular epithelial cells against PQ toxicity through suppressing oxidative stress and mitochondrial damage, and these protective effects were reversed by Tollip shRNA . Collectively, the present study demonstrated that IRN ameliorated PQ-induced AKI by attenuating oxidative stress and mitochondrial damage through upregulating Tollip, which provides new insights into the pathogenesis and treatment of PQ poisoning.
研究领域:
药理学
·
毒理学
·
肾脏生理学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2020
·Vol. 12
·期 37
·4562-4571
·
IF 2.596
Wen Liu,Sha Li,Yang Ke Wu,Xiao Yan,Yi Ming Zhu,Fei Ya Jiang,Yu Jiang,Liang Hong Zou,Tong Tong Wang
Xuebijing (XBJ) is a compound Chinese medicine that contains Paeoniae Radix Rubra, ChuanXiong Rhizoma, Salvia Miltiorrhiza Radix et Rhizoma, Carthami Flos, and Angelicae Sinensis Radix. It is widely used in China to treat sepsis. Previous studies have demonstrated that XBJ can decrease mortality in patients with moderate paraquat poisoning. However, the mechanism by which it exerts this effect is not completely clear. In this study, an ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS/MS)-based metabolomics approach was used to perform a metabolic profiling analysis. Principal component analysis (PCA), random forest (RF), and partial least squares discriminant analysis (PLS-DA) were used to identify metabolites to clarify the mechanism of XBJ's activity. XBJ clearly alleviated lung injury in a Sprague Dawley (SD) rat model of paraquat (PQ) poisoning. Seven metabolites related to four pathways, including those involved in sphingolipid and phospholipid metabolism, amino acid metabolism, unsaturated fatty acid metabolism, and pantothenic acid and CoA biosynthesis, were present at different levels in PQ-poisoned rats treated with XBJ compared with untreated rats. XBJ can ameliorate the effects of PQ poisoning in SD rats. Using a metabolomics approach enabled us to gain new insight into the mechanism underlying this effect.
研究领域:
药理学
·
毒理学
·
代谢组学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）