JOURNAL OF CHROMATOGRAPHY A
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2026
·
IF 4
Deng Li,Yan Li,Yingxi Zhu,Zhou Li,Die Gao,Jia Zeng,Dan Li,Xiaodan Hu,Jiahao Lin,Xiongwei Yin,Famin Ke,Wei Ren,Dandan Wang
A MXene-based molecularly imprinted polymer (MXMIP) composite was developed for the enrichment and detection of puerarin and its metabolites in complex biological samples. As an ideal substrate material, MXene can make the recognition sites within the MIP matrix more easily exposed compared to conventional MIP. Through the operation of the "dual screening" mechanism, MXMIP combines interaction enhancement with molecular recognition, significantly enhancing the adsorption capacity and efficiency of this material. The successful synthesis of the composite was confirmed by comprehensive characterization of structure and properties. MXMIP achieved a maximum adsorption capacity of 3.70 mg∙g –1 , higher than conventional MIP (2.24 mg∙g –1 ) and MXene alone (1.74 mg∙g –1 ). The adsorption capacity of MXMIP was 1.65 times that of conventional MIP and 2.12 times that of MXene. In selective adsorption experiments, MXMIP shows excellent specificity toward puerarin, with selectivity factors ranging from 1.33 to 10.4 relative to structural analogs, and achieved an imprinting factor of 6.46. The hydrogen bonding interactions between MXMIP and puerarin were confirmed by ultraviolet spectroscopy, Fourier transform infrared spectroscopy, and density functional theory simulations. MXMIP was successfully applied to enrich and detect puerarin and its metabolites in biological samples. LC-MS/MS analysis showed that the mass charge ratios of MXMIP adsorbed samples were 415.09, 447.13, 253.05 and 255.23, respectively, corresponding to puerarin and its metabolites (dihydroxypuerarin, daidzein and dihydrodaidzein). This study provides a new approach for synthesizing highly selective molecularly imprinted polymers that effectively address the problem of binding site interception.
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PubMed
高影响因子
ELECTROANALYSIS
·
2021
·Vol. 34
·期 12
·1877-1883
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IF 3.223
Yuan Xue,Yong Xia,Yanchao Han,Chao Peng,Jing Li,Erkang Wang
The development of the hot electron-induced cathodic electrochemiluminescence (HECL) often accompanied with the advance in the electrode materials and instruments. In this work, dimethyl silicone oil was employed as the coating layer for initiating the HECL. The insulating layer was obtained by simple drop casting and subsequent polishing, which could withstand high voltage and meet the requirement of HECL. With the as-prepared interface, the quantitative detection of puerarin in the aqueous solution was achieved based on the quenching effect on the SO 4 ⋅ − .
研究领域:
分析化学
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电化学
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高影响因子
JOURNAL OF LIPOSOME RESEARCH
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2026
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IF 4.7
Pu Yang,Xiqi Wu,Jingjian Liu,Tarun Belwal,Luyao Qi,Xiaotian Li,Jingjing Liang,Simin Feng,Liuqing Yang
Puerarin (Pue), an isoflavone with diverse pharmacological activities, exhibits poor oral bioavailability due to its low solubility and permeability. To improve its oral delivery, phytosterol ester (PE) was employed as a membrane stabilizer to replace cholesterol (CH) in liposomal formulations. Two Pue-loaded nanoliposomes, CH-Pue-NLs and PE-Pue-NLs, were successfully prepared via ethanol injection. PE-Pue-NLs exhibited a particle size of 219.80 ± 1.81 nm, a zeta potential of 48.76 ± 1.65 mV, and an encapsulation efficiency (EE) of 74.59 ± 1.25%, outperforming CH-Pue-NLs. FTIR and DSC analyses confirmed successful encapsulation of Pue. During 15 days of storage at 4 °C, PE-Pue-NLs showed superior physical stability, maintaining PDI < 0.3, zeta potential > +35 mV, and higher EE (59.42 ± 1.17%) than CH-Pue-NLs, which exhibited marked aggregation and drug leakage. In simulated gastrointestinal fluids, PE-Pue-NLs demonstrated enhanced structural stability and a slower biphasic release profile, with a lower cumulative release in simulated intestinal fluid at 6 h (36.87 ± 1.47%) compared with CH-Pue-NLs (50.29 ± 2.57%). Furthermore, PE-Pue-NLs exhibited reduced binding with mucin and a 1.32-fold higher apparent permeability coefficient (Papp) in Transwell studies, indicating enhanced mucus penetration. In vivo intestinal fluorescence imaging further confirmed improved mucosal permeability of PE-Pue-NLs. These findings suggest that phytosterol ester is a promising alternative to cholesterol for constructing liposomes with improved gastrointestinal stability and intestinal permeability for poorly soluble bioactive compounds.
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高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Puxuan Zhao,Tianqi Liu,Yanan Shi,Zhiming Li,Shaojie Lu,Fei Jia,Hao Chang
Methotrexate (MTX) is a primary treatment for moderate to severe psoriasis, but conventional oral or subcutaneous delivery has limitations, including poor drug targeting, side effects, and low patient compliance. This study introduces implantable hydrogel microneedles (HMNs) made from photo-cross-linked gelatin methacryloyl (GelMA) and puerarin (Pue), a traditional Chinese medicine, for intradermal and sustained MTX delivery. The ratio of Pue to GelMA significantly influenced the morphology, mechanical strength, MTX release profiles, and degradation behavior of the HMNs. After insertion into the skin, the backing layer of the microneedles, which incorporates effervescent agents (NaHCO 3 and citric acid), rapidly generates gas bubbles (CO₂) upon contact with skin interstitial fluid, facilitating the immediate separation of the HMNs from the backing and their implantation into skin tissue as MTX reservoirs. HMNs with 1.5 % w / v Pue and 20 % w/v GelMA achieved a 10-day MTX release in vivo . In vivo studies demonstrate that MTX-loaded Pue/GelMA HMNs significantly enhance therapeutic outcomes in alleviating psoriasis symptoms and downregulating psoriasis-associated cytokines due to the synergistic effects of MTX and Pue, outperforming oral administration, subcutaneous injection, and GelMA HMNs containing either Pue or MTX alone. This HMN patch offers great promise for improving psoriasis treatment and patient adherence.
研究领域:
药学
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皮肤科
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药物递送系统
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生物医学工程
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PubMed
高影响因子
InfoMat
·
2024
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IF 22.7
Chang Su,Jiajia Shao,Zeyang Yu,Al Mahadi Hasan,Chengmin Bao,Chris R. Bowen,Chuanbo Li,Zhong Lin Wang,Ya Yang
The detection of puerarin concentration is an essential capability to study the functional role of the Pueraria root as a natural medicine and dietary source in the treatment of cardiovascular diseases and liver protection. Current methods to detect and measure puerarin concentration, such as ultraviolet–visible spectrophotometry (UV), are bulky, require an external power supply, and are inconvenient to use. Here, we propose a triboelectric puerarin-detecting sensor (TPDS) which is based on liquid–solid contact electrification, in which liquid–solid interactions generate rapid electrical signals in only 0.4 ms to enable real-time detection of puerarin concentration in water droplets. The electrical signal of the TPDS decreases with an increase of puerarin concentration, and the sensitivity of the approach is 520 V·(μg/mL) −1 . The TPDS represents a miniature and cost-effective sensor that is 0.2% of the size and 0.01% of the cost of a UV spectrophotometer. Our theoretical analysis verified that the puerarin concentration in droplets can effectively regulate the electronic structure, where higher concentrations of puerarin lead to a narrower energy bandgap, which allows the TPDS to detect puerarin concentration without the need for an external power supply. The TPDS therefore provides a route for the development of a portable and self-powered method to measure the concentration of an active ingredient in droplets through the conversion of natural energy.
研究领域:
生物医学工程
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分析化学
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纳米技术
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材料科学
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药学
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高影响因子
Bioactive Materials
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2022
·
IF 16.874
Liping Ouyang,Baohui Chen,Xingdan Liu,Donghui Wang,Yang Li,Yun Liao,Kelvin W.K. Yeung,Xuanyong Liu
It is important to eliminate lipopolysaccharide (LPS) along with killing bacteria in periprosthetic joint infection (PJI) therapy for promoting bone repair due to its effect to regulate macrophages response. Although natural antimicrobial peptides (AMPs) offer a good solution, the unknown toxicity, high cost and exogenetic immune response hamper their applications in clinic. In this work, we fabricated a nanowire-like composite material, named [email protected] , by combining chitosan and puerarin via solid-phase reaction, which can finely mimic the bio-functions of AMPs. Chitosan, serving as the bacteria membrane puncture agent, and puerarin, serving as the LPS target agent, synergistically destroy the bacterial membrane structure and inhibit its recovery, thus endowing [email protected] with good antibacterial property. In addition, [email protected] possesses good osteoimmunomodulation due to its ability of LPS elimination and macrophage differentiation modulation. The in vivo results show that [email protected] can inhibit the LPS induced bone destruction in the Escherichia coli infected rat. [email protected] exhibits superior bone regeneration in Escherichia coli infected rat due to the comprehensive functions of its superior antibacterial property, and its ability of LPS elimination and immunomodulation. [email protected] can well mimic the functions of AMPs, which provides a novel and effective method for treating the PJI in clinic.
研究领域:
医学
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生物材料学
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PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2025
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IF 5.4
Yibiao Zhou,Xiaowei Yang,Chao Deng,Yongjie Xia,Shengping Tang,Hongwu Zeng,Bin Zhang,Xiaoyong Zhang,Guibing Fu,Chao You
Osteoporosis (OP) is a degenerative bone disease that commonly occurs in elderly people. It often manifests in bone pain and increased risk of fractures, significantly impacting the quality of life for middle-aged and elderly individuals. Several anti-osteoporosis drugs are currently available in clinical practice, their long-term use has shown limited efficacy and potential complications. The natural compound puerarin (Pue) has demonstrated to be promising for OP treatment via regulating osteoclast and osteoblast differentiation. However, its poor solubility, low bioavailability, and lack of bone-targeting severely impeded its clinical applications. To address the above issues, we reported herein the synthesis of Pue-based framework (HCCP-Pue-PEG-ALN, HPPA NPs) via conjugation of Pue, NH 2 -PEG, and alendronic acid (ALN) using hexachlorocyclotriphosphazene (HCCP) as the linkage. We demonstrated that the HPPA NPs display the features, including high water dispersibility, pH responsive drug release, low cytotoxicity, bone targeting feature, and positive effects on both osteoclasts and osteoblasts. Therefore, we believe this idea reported in this work will be of great interest in design natural compounds-based drug delivery systems and promotes clinic translation of natural compounds for OP treatment.
研究领域:
药学
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生物医学工程
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高影响因子
BIOMEDICINE & PHARMACOTHERAPY
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2024
·
IF 6.9
Lili Zhang,Chengcheng Duan,Shiquan Feng,Beicheng Zhao,Hailong Li,Xi Zhang,Yan Zhou,Zhenmiao Qin
Background Puerarin (Pue) is an isoflavone compound with significant therapeutic effect on cardiovascular diseases, but its poor water solubility and low oral bioavailability limit clinical application. Methods In this study, Pue was prepared into PLGA nanoparticles (Pue-PLGA NPs) by emulsion solvent volatilization method. The morphology, particle size, Zeta potential, X-ray diffraction (XRD), and fourier transform infrared (FTIR) of the NPs were characterized. Additionally, their stability and in vitro release were evaluated. SD rats were orally administered wtih Pue and Pue-PLGA NPs, and a high performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was established to determine the concentration of blood samples and to investigate the pharmacokinetic behaviour of Pue and Pue-PLGA NPs in rats. Results The NPs were observed by transmission electron microscopy (TEM) as regular spheroids and uniformly dispersed. The average particle size of the NPs was (167.1±5.26）nm, the Zeta potential was (-29.88±2.46)mV, the encapsulation rate was (83.12 %±4.73 %) and the drug loading capacity was (7.75 %±1.81 %). The results of in vitro release showed that the drug was released slowly and continuously from the NPs, reaching the release platform in 24 h, and the cumulative release amount was (88.55±2.86) %. The pharmacokinetic results showed that the AUC 0–24 , AUC 0-∞ , C max , T max , t 1/2 , MRT 0–24 and MRT 0-∞ of Pue-PLGA NPs were 2.196, 1.978, 1.327, 1.5, 1.385, 3.915 and 3.140 times of Pue, respectively. The relative bioavailability was (197.82±25.28) %. Conclusion These results indicated that the prepared nanoparticles had small particle size, high encapsulation rate, drug loading capacity and good slow-release effect, and could significantly improve the oral bioavailability of Pue in rats.
研究领域:
药剂学
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药物递送系统
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生物医学工程
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高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Hao Yuan,Zhaoxiang Zeng,Dongru Li,Rongzeng Huang,Wan Li
Puerarin (PUE), a natural and biologically active isoflavone extracted from Chinese medicine Pueraria lobata , can self-assemble to form a hydrogel without other chemical modifications. However, although PUE hydrogel has pH responsivity, but it is difficult to adapt to the changeable pathological environment. Therefore, thiolated chitosan (TCS) is synthesized and hybridized with PUE hydrogel to prepare TCS 10 /PUE composite hydrogel. The results of rheological measurement showed that the resultant composite hydrogels inherited the low loss performance of TCS hydrogel, which means that they have stronger elasticity. Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) images displayed that TCS 10 /PUE composite hydrogel has a fibrous-network structure. X-Ray Diffractometer (XRD) and Fourier transform infrared spectroscopy (FT-IR) proved the existence of hydrogen bonds and disulfide bonds in the formation of composite hydrogel. Degradation experiment showed that TCS 10 /PUE composite hydrogels have pH and glutathione (pH/GSH) dual sensitivity. Furthermore, TCS 10 /PUE composite hydrogels exhibited multi-functionality including thixotropy, cytocompatibility, antibacterial and anti-inflammatory properties. Berberine chloride hydrate (BCH) was further used as a model drug for in vitro release study. BCH and PUE could be released cooperatively under pH/GSH dual responsivity. These results indicated that the resultant composite hydrogel has eminent pH/GSH dual responsivity and could act as a potential new intelligent drug carrier.
研究领域:
材料科学
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药剂学
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生物医学工程
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纳米技术
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PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2020
·
IF 7.053
Yejun Zhong,Jincheng Zhao,Taotao Dai,David Julian McClements,Chengmei Liu
In this study, the impact of protein-polyphenol interactions on the formation of heat-set protein hydrogels was investigated. Whey protein isolate (WPI) was used as a model globular protein, while puerarin (PUE) was used as a model polyphenol. Fluorescence spectroscopy combined with molecular docking simulations suggested that the main interaction between WPI and PUE was hydrogen bonding. The rheological properties of the hydrogels could be modulated by altering the polyphenol concentration (0.3–0.9%). In particular, the complex shear modulus, hardness, and resilience of the hydrogels increased with increasing PUE level. This research provides valuable information about the impact of protein-polyphenol interactions on hydrogel formation, which may be useful for the fortification of functional foods with polyphenol-based nutraceuticals like puerarin.
研究领域:
食品科学
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生物化学
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高影响因子
JOURNAL OF MATERIALS SCIENCE
·
2020
·Vol. 55
·期 15
·6669-6677
·
IF 3.553
Li Wan
Hydrogels as drug carriers for delivery of antibiotics are rarely derived from natural molecules and often require complicated synthesis. Puerarin is an herbal natural product that can self-assemble to form pH-sensitive hydrogels without any structural modification. However, Puerarin hydrogels have poor thermostability and weak mechanical strength. In this work, we prepared N -(9-fluorenylmethoxycarbonyl)- l -phenylalanine (Fmoc–Phe–OH) nanofiber-reinforced Puerarin hydrogels with good mechanical and antibacterial properties for suitable drug carriers. The phase transition temperature of Puerarin hydrogels can be increased from 35 to 55 °C by adding only 6 mg/mL of Fmoc–Phe–OH. Rheological study indicated that the presence of Fmoc–Phe–OH not only increased the storage modulus of Puerarin hydrogels by five times, but also did not affect their pH sensitivity. SEM study and pore analysis indicated that Fmoc–Phe–OH nanofibers intertwined with the Puerarin assembly, leading to enhancement of mechanical property of hydrogel. Antibacterial test indicated that the reinforced Puerarin hydrogels have antibacterial property compared with Puerarin hydrogels. Based on these features, a controllable release of loaded model drug (berberine hydrochloride) can be realized from the reinforced Puerarin hydrogels, showing a good synergistic antimicrobial effect on the S. aureus .
研究领域:
药学
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材料科学
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生物医学工程
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高影响因子
TALANTA
·
2012
·
IF 3.794
Lizhen Liu,Feng Feng,Shaomin Shuang,Yunfeng Bai,Martin M.F. Choi
A rapid and efficient capillary zone electrophoresis (CZE) method with ultraviolet detection has been successfully developed for the determination of puerarin in Xinkeshu capsules and biological samples. The optimal CZE conditions were determined to be 70 mM H 3 BO 3 –Na 2 B 4 O 7 , pH 9.2, injection time 5 s, applied voltage 25 kV, working temperature 25 °C and detection wavelength 254 nm. Under these conditions, a linear range from 17.3 to 138 μg/mL with the correlation coefficient of 0.9998 and limit of detection of 34.6 ng/mL ( S / N = 3) for puerarin were obtained. The relative standard deviation of the migration time and peak area of puerarin were 0.23–0.91, 0.43–1.62, 0.10–0.64 and 0.64–1.32% for intra-day and inter-day analyses, respectively. Satisfactory results were obtained for recovery and repeatability. The proposed method was successfully applied to analyze the puerarin content in Xinkeshu capsules and some biological samples with good results. It is suitable for applications in pharmaceutical industries for quality control and in clinical laboratories for therapeutic drug monitoring purposes.
研究领域:
药学分析
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生物分析化学
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高影响因子
危险品化学品经营许可证（带存储）