MOLECULES
·
2023
·Vol. 28
·期 14
·5309
·
IF 4.6
Shi-Hui Lu,Xiao-Na Liang,Xiao-Jin Nong,Ran Chen,Xiu-Xia Li
Trans-p-hydroxycinnamic acid and its esters in the leaves ofLigustrum robustummight be a new resource to prevent diabetes and its complications. In the present study, a new HPLC-UV method using hydrolyzation with sodium hydroxide for quantitation oftrans-p-hydroxycinnamic acid and totaltrans-p-hydroxycinnamic acid esters in the leaves ofL. robustumwas developed, since it was difficult and troublesome to analyze no less than 34trans-p-hydroxycinnamic acid esters by usual HPLC. The extract ofL. robustumwas hydrolyzed with sodium hydroxide at 80 °C for 2 h, and then, hydrochloride was added. HPLC analysis was performed in reverse phase mode using a C-18 column, eluting with methanol-0.1% acetic acid aqueous solution (40:60,v/v) in isocratic mode at a flow rate of 1.0 mL·min−1and detecting at 310 nm. The linear range fortrans-p-hydroxycinnamic acid was 11.0–352.0 μg·mL−1(r2= 1.000). The limit of detection and limit of quantification were 2.00 and 6.07 μg·mL−1, respectively. The relative standard deviations of intra-day and inter-day variabilities fortrans-p-hydroxycinnamic acid were less than 2%. The percentage recovery oftrans-p-hydroxycinnamic acid was 103.3% ± 1.1%. It is the first HPLC method using hydrolyzation for quantification of many carboxylic esters. Finally, the method was used successfully to determinetrans-p-hydroxycinnamic acid and totaltrans-p-hydroxycinnamic acid esters in various extracts of the leaves ofL. robustum. The 60–70% ethanol extracts ofL. robustumshowed the highest contents of freetrans-p-hydroxycinnamic acid (3.96–3.99 mg·g−1), and the 50–80% ethanol extracts ofL. robustumdisplayed the highest contents of totaltrans-p-hydroxycinnamic acid esters (202.6–210.6 mg·g−1). The method can be applied also to the quality control of the products ofL. robustum.Keywords:trans-p-hydroxycinnamic acid;esters;Ligustrum robustum;HPLC-UV;hydrolyzation;sodium hydroxide;quantification
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高影响因子
Humanities & Social Sciences Communications
·
2026
·
IF 4.8
Wen Bing,Lin Zhong
Drawing on translanguaging as a theoretical lens, this article reconceptualizes mistranslation, pseudotranslation, and wandering translation as distinct yet interrelated modes of trans-translating. Examining examples from colonial encounters, feminist writing, and digital culture, it shows how these practices enact epistemic resistance and cultural innovation, subverting linguistic hierarchies and amplifying marginalized voices. Trans-translating transcends source-target binaries to foster transcultural dialogue—yet this dialogue is not politically neutral. Its significance lies in what we theorize as situated accountability: a heuristic that assesses any translational act by asking who it serves, with what intention, under what conditions for reinterpretation, and through what counter-hegemonic spaces. By grounding ethical judgment in these concrete factors, the framework advances equitable exchange not by celebrating fluidity wholesale but by distinguishing emancipatory enactments from harmful ones.
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ISME Journal
·
2024
·
IF 10.8
Zheng Cao,Liu Dingqi,Lu Xinyu,Wu Huijun,Hua Jingyi,Zhang Chuang,Liu Kang,Li Changchun,He Jin,Du Cuiying
The ability of bacteria to use natural carbon sources greatly affects their growth and survival in the environment. Bacteria have evolved versatile abilities to use environmental carbon sources, but their diversity and assimilation pathways remain largely unexplored. Trans-aconitic acid, a geometric isomer of cis-aconitic acid involved in the tricarboxylic acid cycle, has long been considered a natural carbon source metabolizable by bacteria. However, its catabolism and ecological role in linking bacterial interactions with the environments remain unclear. Here, we identify a regulatory system in Bacillus velezensis FZB42 that is capable of sensing and catabolizing trans-aconitic acid. The system consists of a tar operon, an adjacent positive regulatory gene tarR, and a shared promoter. After receiving the trans-aconitic acid signal, the TarR protein interacts directly with the promoter, initiating the expression of the membrane transporter TarB and aconitate isomerase TarA encoded by the operon, which function in importing the trans-aconitic acid and isomerizing it into the central intermediate cis-aconitic acid. Subsequent soil colonization experiments reveal that trans-aconitic acid assimilating ability can give its coding bacteria a growth and competitive advantage. Bioinformatics analyses coupled with bacterial isolation experiments further show that the assimilation system of trans-aconitic acid is widely distributed in the bacterial domain, and its assimilating bacteria also extensively distributed in nature, indicating an important role of trans-aconitic acid metabolism in bacterial carbon acquisition. This work emphasizes the importance of metabolic adaptation to environmental carbon sources for bacterial survival and may provide inspiration for engineering microbes with enhanced environmental competitiveness.
研究领域:
微生物学
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环境科学
·
分子生物学
·
生态学
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JOURNAL OF CONTROLLED RELEASE
·
2024
·
IF 10.5
Qiuyu Wei,Chenchen Zhu,Guiping Yuan,Jiahui Jin,Jing Zhang,Wufa Fan,Ying Piao,Shiqun Shao,Sen Lin,Jiajia Xiang,Youqing Shen
Efficient and noninvasive drug delivery for glaucoma therapy necessitates prolonged retention on the ocular surface and deep penetration into the cornea. However, inherent physiological defenses such as rapid tear clearance and low cornea permeability present significant challenges that hinder the effectiveness of trans-corneal drug delivery. In this study, we demonstrate the potential of zwitterionic micelles composed of poly(2-(N-oxide-N,N-diethylamino)ethyl methacrylate)- block -poly(ε-caprolactone) (OPDEA-PCL) amphiphiles as a biocompatible carrier for trans-corneal drug delivery. These micelles exhibit enhanced adhesion to ocular tissues and resistance to tear clearance due to their unique affinity for cell membranes. These characteristics facilitate adsorptive-mediated transcytosis, significantly augmenting trans-corneal transport and intraocular accumulation of the glaucoma medication brinzolamide (BRZ). As a result, OPDEA-PCL/BRZ formulations effectively normalize intraocular pressure in an open-angle glaucoma rat model, surpassing PEGylated and free BRZ formulations. This research underscores the potential utility of OPDEA-PCL micelles as a promising vehicle for noninvasive topical trans-corneal drug delivery in glaucoma therapy.
研究领域:
药剂学
·
眼科
·
药物递送系统
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高影响因子
Materials Today Communications
·
2024
·
IF 3.7
Pingping Wu,Xuegang Hao,Yuanyuan Shang,Junchao Liu
Azobenzene, a representative photoresponsive liquid crystal, has extensive applications in fields such as bionic robotics, actuators, artificial muscles, motors, and various functional devices. Photoisomerization ( trans-cis isomerization) is the most important mechanism for its photoresponse. Currently, the isomerization of azobenzene mesogens can be measured through UV–vis absorption spectra, NMR techniques or transient grating methods. Visualization of the trans-cis isomerization of azobenzene mesogens still remains a challenge. In this study, a linear relationship is observed between the absorption intensity of azobenzene mesogens and the stopband shift of azobenzene inverse opals (AZOIOs) films during the isomerization process. This phenomenon can be used to determine the isomerization degree of azobenzene mesogens through the switching of the stopband of the AZOIOs film, enabling the visualization of the azobenzene mesogens’s configuration transition. The results of this study are highly significant for the design and fabrication of novel azobenzene devices.
研究领域:
材料科学
·
光学
·
化学
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高影响因子
iScience
·
2022
·
IF 6.107
Kefeng Wang,Qingzhen Yu,Wenli Mao,Yuxin Zheng,Jing Xu,Yukun Wang
Vinylboron compounds are important compounds in organic chemistry and biology. In this communication, we developed a copper(I)-catalyzed, highly regio- and stereoselective radical trans -hydroboration of ynamides with N -heterocyclic carbene (NHC)-ligated borane is reported, which leads to a series of trans -boryl enmides that can be conveniently transformed into various multi-substituted enamides. Further investigation showcased that our method is robust and scalable. The mechanism of this unique reaction is studied and discussed.
研究领域:
有机化学
·
合成化学
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高影响因子
Microbial Cell Factories
·
2024
·Vol. 23
·期 1
·1-16
·
IF 4.3
Wang Yifan,Shu Dan,Li Zhemin,Luo Di,Yang Jie,Chen Dongbo,Li Tianfu,Hou Xiaonan,Yang Qi,Tan Hong
Background Currently, industrial fermentation of Botrytis cinerea is a significant source of abscisic acid (ABA). The crucial role of ABA in plants and its wide range of applications in agricultural production have resulted in the constant discovery of new derivatives and analogues. While modifying the ABA synthesis pathway of existing strains to produce ABA derivatives is a viable option, it is hindered by the limited synthesis capacity of these strains, which hinders further development and application. Results In this study, we knocked out the bcaba4 gene of B. cinerea TB-31 to obtain the 1′,4′- trans -ABA-diol producing strain ZX2. We then studied the fermentation broth of the batch-fed fermentation of the ZX2 strain using metabolomic analysis. The results showed significant accumulation of 3-hydroxy-3-methylglutaric acid, mevalonic acid, and mevalonolactone during the fermentation process, indicating potential rate-limiting steps in the 1′,4′- trans -ABA-diol synthesis pathway. This may be hindering the flow of the synthetic pathway. Additionally, analysis of the transcript levels of terpene synthesis pathway genes in this strain revealed a correlation between the bchmgr , bcerg12 , and bcaba1-3 genes and 1′,4′- trans -ABA-diol synthesis. To further increase the yield of 1′,4′- trans -ABA-diol, we constructed a pCBg418 plasmid suitable for the Agrobacterium tumefaciens -mediated transformation (ATMT) system and transformed it to obtain a single-gene overexpression strain. We found that overexpression of bchmgr , bcerg12 , bcaba1 , bcaba2 , and bcaba3 genes increased the yield of 1′,4′- trans -ABA-diol. The highest yielding ZX2 A3 strain was eventually screened, which produced a 1′,4′- trans -ABA-diol concentration of 7.96 mg/g DCW (54.4 mg/L) in 144 h of shake flask fermentation. This represents a 2.1-fold increase compared to the ZX2 strain. Conclusions We utilized metabolic engineering techniques to alter the ABA-synthesizing strain B. cinerea , resulting in the creation of the mutant strain ZX2, which has the ability to produce 1′,4′- trans -ABA-diol. By overexpressing the crucial genes involved in the 1′,4′- trans -ABA-diol synthesis pathway in ZX2, we observed a substantial increase in the production of 1′,4′- trans -ABA-diol.
研究领域:
生物技术
·
微生物学
·
代谢工程
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高影响因子
Research
·
2022
·
IF 11.036
Liangfen Cheng,Fuhan Yang,Longfei Tang,Lelin Qian,Xu Chen,Feng Guan,Juan Zhang,Genxi Li
Evaluating tumor development is of great importance for clinic treatment and therapy. It has been known that the amounts of sialic acids on tumor cell membrane surface are closely associated with the degree of cancerization of the cell. So, in this work, cellular interface supported CRISPR/Cas trans-cleavage has been explored for electrochemical simultaneous detection of two types of sialic acids, i.e., N-glycolylneuraminic acid (Neu5Gc) and N-acetylneuraminic acid (Neu5Ac). Specifically, PbS quantum dot-labeled DNA modified by Neu5Gc antibody is prepared to specifically recognize Neu5Gc on the cell surface, followed by the binding of Neu5Ac through our fabricated CdS quantum dot-labeled DNA modified by Sambucus nigra agglutinin. Subsequently, the activated Cas12a indiscriminately cleaves DNA, resulting in the release of PbS and CdS quantum dots, both of which can be simultaneously detected by anodic stripping voltammetry. Consequently, Neu5Gc and Neu5Ac on cell surface can be quantitatively analyzed with the lowest detection limits of 1.12 cells/mL and 1.25 cells/mL, respectively. Therefore, a ratiometric electrochemical method can be constructed for kinetic study of the expression and hydrolysis of Neu5Gc and Neu5Ac on cell surface, which can be further used as a tool to identify bladder cancer cells at different development stages. Our method to evaluate tumor development is simple and easy to be operated, so it can be potentially applied for the detection of tumor occurrence and development in the future.
研究领域:
生物医学工程
·
分子生物学
·
肿瘤学
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高影响因子
Heliyon
·
2018
·
IF 0
Ningning Cheng,Jingjing Zhang,Jingmei Yin,Shenmin Li
The harm of trans-fatty acids to health has aroused public concern. It is believed that the main source of trans-fatty acids in diets is the isomerization of unsaturated fatty acids in edible oils during cooking. However, the information on the isomerization mechanism is very limited. In this paper, we used oleic acid, an unsaturated fatty acid, as a simplified model for edible oil and investigated the mechanism of cis/trans isomerization by computation and experiments. The computational results show that R c -O-O-H is a very important intermediate, and the cleavage of O-O bond in R c -O-O-H is the rate-controlling step during the cis/trans isomerization. Using the ATR-FTIR measurements, the contents of elaidic acid were measured quantitatively in sites. The experimental results indicate that the cis/trans isomerization of oleic acid can occur obviously only under oxidizing condition when the temperature is higher than 120 °C.
研究领域:
食品化学
·
计算化学
·
物理有机化学
·
营养科学
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PubMed
JOURNAL OF BIOLOGICAL CHEMISTRY
·
2021
·Vol. 298
·期 2
·
IF 5.157
Binxiang Cai,Chunyan Liao,Danxue He,Jingmeng Chen,Jiahuai Han,Jiaying Lu,Kaiqi Qin,Wenxu Liang,Xiaoling Wu,Zuguo Liu,Yalin Wu
The breakdown of all-trans-retinal (atRAL) clearance is closely associated with photoreceptor cell death in dry age-related macular degeneration (AMD) and autosomal recessive Stargardt's disease (STGD1), but its mechanisms remain elusive. Here, we demonstrate that activation of gasdermin E (GSDME) but not gasdermin D promotes atRAL-induced photoreceptor damage by activating pyroptosis and aggravating apoptosis through a mitochondria-mediated caspase-3-dependent signaling pathway. Activation of c-Jun N-terminal kinase was identified as one of the major causes of mitochondrial membrane rupture in atRAL-loaded photoreceptor cells, resulting in the release of cytochrome c from mitochondria to the cytosol, where it stimulated caspase-3 activation required for cleavage of GSDME. Aggregation of the N-terminal fragment of GSDME in the mitochondria revealed that GSDME was likely to penetrate mitochondrial membranes in photoreceptor cells after atRAL exposure. ABC (subfamily A, member 4) and all-trans-retinol dehydrogenase 8 are two key proteins responsible for clearing atRAL in the retina. Abca4−/−Rdh8−/− mice exhibit serious defects in atRAL clearance upon light exposure and serve as an acute model for dry AMD and STGD1. We found that N-terminal fragment of GSDME was distinctly localized in the photoreceptor outer nuclear layer of light-exposed Abca4−/−Rdh8−/− mice. Of note, degeneration and caspase-3 activation in photoreceptors were significantly alleviated in Abca4−/−Rdh8−/−Gsdme−/− mice after exposure to light. The results of this study indicate that GSDME is a common causative factor of photoreceptor pyroptosis and apoptosis arising from atRAL overload, suggesting that repressing GSDME may represent a potential treatment of photoreceptor atrophy in dry AMD and STGD1.
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Communications Biology
·
2026
·
IF 5.8
Wang Yan,Zhu Yanping,Liu Ning-Hua,Zhao Fang,Cao Hai-Yan,Chen Yin,Zhang Yu-Zhong,Chen Xiu-Lan,Zhang Yu-Qiang,Gao Chao
Free trans -4-hydroxy-L-proline (T4LHyp), primarily released during the natural degradation of collagen, is absorbed and catabolised by heterotrophic bacteria, contributing to the global recycling of carbon and nitrogen. While bacterial T4LHyp catabolism is well documented, our understanding on how bacteria recognize and import T4LHyp remains limited. Here we show that HatA, the substrate binding protein (SBP) of an ATP-binding cassette transporter within the T4LHyp gene cluster of Vibrio alginolyticus 1A11092, recognizes T4LHyp during its uptake by the strain. HatA possessed significant binding affinity towards T4LHyp and L-proline and a binding signature (W 33 F 37 D 86 V 135 F 240 R 243 Y 265 N 267 Y 303 ), acting as the structural determinant for the specific ligand recognition, was defined. This signature is strictly conserved among HatA and its homologs, representing a subgroup of cluster D-II of SBPs. Genes encoding HatA-like SBPs are often located adjacent to collagenase and T4LHyp catabolic enzyme genes, and are nearly exclusively found in the genomes of Vibrio spp. in hosts and marine environments, which helps them exert pathogenicity when encountering host or environmental collagen. This study reveals a T4LHyp SBP in cosmopolitan Vibrio spp., offering better insights into the global T4LHyp catabolism and recycling driven by bacteria.
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高影响因子
Journal of Fungi
·
2023
·Vol. 9
·期 9
·930
·
IF 4.7
Qiuli Ouyang,Shiwei Shi,Yangmei Liu,Yanqin Yang,Yonghua Zhang,Xingxing Yuan,Nengguo Tao,Lu Li
Geotrichum citri-aurantii(G. citri-aurantii) is one of the most important postharvest pathogens leading to a postharvest loss of citrus by causing sour rot. In this study, the antifungal activity oftrans-2-hexenal, a natural component of essential oil, againstG. citri-aurantiiwas evaluated.Trans-2-hexenal treatment inhibited the mycelia growth ofG. citri-aurantiiwith a minimum inhibitory concentration and minimum fungicidal concentration oftrans-2-hexenal at 0.50 and 1.00 μL/mL, respectively. Moreover,trans-2-hexenal efficiently reduced the incidence of sour rot of Satsuma fruit inoculated withG. citri-aurantii. Ultrastructural observations and Fourier transform infrared (FT−IR) results showed thattrans-2-hexenal treatment affected the cell wall and cell membrane instructions ofG. citri-aurantii. The content ofβ-1,3-glucan was significantly decreased aftertrans-2-hexenal treatment, but the cell wall permeability was not changed. The decrease in lipid and ergosterol contents might be responsible for this antifungal activity. Several important genes,FKS1,ERG1,ERG7, andERG11, showed decreasing expression levels aftertrans-2-hexenal treatment. Molecule-docking results also indicated thattrans-2-hexenal could join with the protein of FKS1, ERG1, ERG7, and ERG11 to impact enzyme activities. These results demonstrated thattrans-2-hexenal is a promising fungicide for controlling sour rot of harvested citrus fruit by damaging the membrane integrity ofG. citri-aurantii.Keywords:G. citri-aurantii;trans-2-hexeanl;antifungal mechanism;cell wall;cell membrane
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高影响因子
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