INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2019
·
IF 3.375
Jin-Hua Xue,Hui Zhang,D. X. Ding,Nan Hu,Yong-Dong Wang,Yong-Sheng Wang
Linear β-cyclodextrin polymer functionalized multiwalled carbon nanotubes (MWCNTs-CDP) were synthesized and employed as a novel nanoadsorbent to remove uranyl ions in wastewater solutions. The characterization of MWCNTs-CDP, with Fourier transform infrared (FT-IR), X-ray diffraction (XRD), scanning electron microscopy (SEM), and Brunauer–Emmett–Teller (BET), suggested that the CDP was successfully modified on the MWCNTs surfaces. The removal efficiency of uranyl ions in aqueous solution by MWCNTs-CDP was investigated via varying various experimental conditions. We found that the removal of uranyl ions correlated with both pH and temperature, and the optimum pH and temperature were 6.0 and 323.15 K, respectively. The adsorption of uranyl ions was very fleet at the initial 1 h and then reached the adsorption equilibrium after 3 h. The data from the adsorption dynamics experiment could be commendably fitted by the pseudo-second-order model (R2 > 0.982), suggesting that chemisorption might be the rate-controlling mechanism. The Langmuir model (R2 > 0.995) manifested that the maximum adsorption capacity of uranyl ions on MWCNTs-CDP was boosted from 66.16 to 89.54 mg g–1 upon the change of temperature from 293.15 to 323.15 K. This result was better than that of some reported adsorbents. The thermodynamics analysis proved that the adsorption of uranyl ions on MWCNTs-CDP was a spontaneous process and an endothermic one. The adsorption mechanism of uranyl ions on MWCNTs-CDP was verified to be dominated by inner-sphere surface complexation. Our results indicated that MWCNTs-CDP can be utilized as a novel nanoadsorbent for highly effective removal of uranyl ions in actual uranyl-bearing effluents.
研究领域:
环境科学
·
材料科学
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DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2025
·
IF 7.7
Jiahui Li,Yuanyuan Zhang,Zhengshun Wen,Jiangbing Shuai,Xiaofeng Zhang,Jinru Zhou,Aiqian Ye,Linglin Fu
The delayed reporting of milk allergen residue detection results is a significant factor contributing to substantial losses for food production enterprises. Cumbersome operational procedures and high testing costs often lead to the abandonment of tests, resulting in frequent allergic reactions among milk-sensitive populations. Therefore, there is an urgent need for a novel, rapid, and low-cost detection method to mitigate enterprise losses and protect allergic individuals. This study investigates the formation mechanism of convection PCR, driven by Fast Taq polymerase in standard PCR tubes, and develops a fluorescence detection device for convection PCR. Through molecular docking and experimental validation of the trimmed β-LG aptamers, we elucidate their potential multi-site binding mechanism. Utilizing this mechanism, we designed a dual-strand hybridization magnetic bead detection system (dsA-SA-B), employing easily synthesized and amplified aptamer complementary strands as an indicator signal; Signal amplification was achieved within 15 min, while the entire detection process was completed in less than 50 min. This detection method allows for the rational selection of aptamer sequences based on actual testing needs, enabling the detection of hydrolyzed milk powder. The method demonstrated a linear detection range for β-LG from 5 to 100 μg/mL, with a detection limit as low as 0.25 μg/mL, effectively identifying hydrolyzed milk powder. This approach transforms protein detection into nucleic acid detection, and lays a solid foundation for multiplex PCR detection of eight major food allergens, facilitating the widespread detection of milk allergens and ultimately contributing to improved food safety and public health.
研究领域:
分子生物学
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食品安全
·
免疫学
·
生物技术
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分析化学
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DOI
高影响因子
Scientific Reports
·
2017
·Vol. 7
·期 1
·1-11
·
IF 4.259
Zhou Yang,Yuan Shaofei,Liu Qian,Yan Dandan,Wang Yun,Gao Li,Han Juan,Shi Haifeng
In this paper, an efficient and convenient Fe 3 O 4 /PMG/IDA-Ni 2+ nanoparticles that applied to purify and immobilize his-tagged β-glucosidase was synthesized, in which, Fe 3 O 4 /PMG (poly ( N, N’- methylenebisacrylamide-co-glycidyl methacrylate) core/shell microspheres were synthesized firstly using distillation-precipitation polymerization, then iminodiacetic acid (IDA) was used to open epoxy rings on the shell of microspheres to the combination of Ni 2+ . The gene of β-glucosidase that was from Coptotermes formosanus Shiraki was amplified, cloned into the expression vector pET28a with an N-terminal His-tag, and expressed in E.coli BL21. The nanoparticles showed the same purification efficiency as commercial nickel column which was a frequently used method in the field of purifying his-tagged proteins from crude cell lysates. The results indicated that Fe 3 O 4 /PMG/IDA-Ni 2+ nanoparticles can be considered as an excellent purification material. β-glucosidase was immobilized on the surface of Fe 3 O 4 /PMG/IDA-Ni 2+ to form Fe 3 O 4 /PMG/IDA-β-glucosidase by means of covalent bound with imidazolyl and Ni 2+ . The immobilized β-glucosidase exhibited excellent catalytic activity and stabilities compared with free β-glucosidase. In addition, immobilized β-glucosidase can be recycled for many times and retain more than 65% of the original activity. The materials display enormous potential in the aspect of purifying and immobilizing enzyme.
研究领域:
生物化学
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生物技术
·
酶学
·
纳米技术
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DOI
PubMed
高影响因子
CERAMICS INTERNATIONAL
·
2025
·Vol. 51
·期 11
·14746-14754
·
IF 5.6
Boyu Chen,Lijuan Niu,Jianlong Chai,Zhiyuan Bai,Jiayin Zhang,Di Liu,Xuefeng Lu,Yabin Zhu
β-SiC ceramics were prepared by spark plasma sintering at 2200 °C with Y 2 O 3 , MgO , and CeO 2 as sintering additives. Al 2 O 3 was doped based on the above oxides to form a two-component sintering additive to carry out a comparative analysis. The effects of these additives on the microstructure, bulk density, and Vickers hardness of β-SiC were systematically studied. The mechanical properties of the single-component samples were superior to those of the two-component samples. Notably, the single-component sample with Y 2 O 3 , addition exhibited the highest density (98.69 %) and hardness (27.1 GPa), which can be attributed to the appropriate growth of the grains in the single-component sample with Y 2 O 3 . Additionally, the principle of lattice reduction in SiC ceramics was explored using first-principle calculations, further highlighting the advantage of Y 2 O 3 as a single additive for the high-temperature sintering of SiC ceramics.
研究领域:
材料科学
·
陶瓷学
·
力学性能
·
计算材料学
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产品关联
DOI
高影响因子
Redox Biology
·
2019
·
IF 7.793
Shen-Fei Wang,Xin Liu,Mo-Yu Ding,Shuangcheng Ma,Jing Zhao,Ying Wang,Shaoping Li
Reducing agents are crucial for the management of maladaptive inflammation-induced macrophage death and hematopoietic toxicity of chemotherapy. 2- O - β - d -glucopyranosyl- l -ascorbic acid (AA-2 β G), a unique AA (or vitamin C) derivative identified in Lycium barbarum , exhibited enhanced free radical scavenging activity compared with AA and its synthetic derivative AA-2 α G. AA-2 β G protected hydrogen peroxide-induced cell death in murine macrophage RAW264.7 cells. Treatment with AA-2 β G eliminated oxidative stress and the ratio of cellular glutathione to glutathione disulfide more effectively than AA and AA-2 α G. AA-2 β G also significantly reduced the fluorescent intensity of DCFH-DA triggered by chemotherapeutic agent camptotehcin-11 but not fluorouracil. AA, AA-2 α G, and AA-2 β G significantly decreased Keap-1expression, and increased the expression levels of nuclear factor E2-related factor 2 (Nrf2) and heme oxygenase-1. All compounds triggered the nuclear translocation of Nrf2, while the ability of AA-2 β G to enhance the Nrf2-DNA binding affinity was approximately two fold as those of AA and AA-2 α G. Sodium ascorbate cotransporters (SVCT) inhibitors, sulfinpyrazone, phloretin, and 3- O -methyglucose, potently abrogated the free radical scavenging activities of AA, AA-2 α G, and AA-2 β G. The cellular uptake efficacy of AA-2 α G and AA-2 β G was less than 10% of AA, while the inhibition of SVCT with sulfinpyrazone considerably diminished the uptake efficacy of these compounds. AA-2 α G and AA-2 β G are more stable in the Fenton reagents than AA. In summary, AA-2 β G from L. barbarum with excellent free radical scavenging activity is a promising natural AA derivative for further pharmacological evaluation.
研究领域:
药理学
·
免疫学
·
肿瘤学
·
分子生物学
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DOI
PubMed
高影响因子
JOURNAL OF LOSS PREVENTION IN THE PROCESS INDUSTRIES
·
2023
·
IF 3.5
Hongxiang Ou,Yining Ran,Mengli Pan,Honglai Xue
Enhancing polymer flame retardancy is crucial for lowering combustion-related exothermic heat and flue gas production. β-cyclodextrin microencapsulated ammonium polyphosphate (β-CD@APP) were synthesis through in-situ polymerization and combined with 1, 3, 5-tris (2-hydroxyethyl) isocyanurate (THEIC) as a novel intumescent flame retardant (IFR) to enhance the flame retardancy of low density polyethylene (LDPE). The results showed that APP's hydrophobic characteristic increased, which enhanced its polymer dispersibility . Compared with LDPE, the LOI value of β-CD@APP/THEIC/LDPE (LDPE5) was enhanced from 17.5% to 28.7% and the composites could meet the UL-94 V-0 standard. Smoke volume and thermal decomposition rate were reduced. Furthermore, a stable char layer was found, which can be seen from the SEM of the char slag and the Raman results. According to the above results, the thermal decomposition and possible mechanism of flame retardants in LDPE composites during combustion were studied.
研究领域:
材料科学
·
聚合物化学
·
消防安全工程
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DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2023
·
IF 8.6
Chaogang Ban,Yang Wang,Jiangping Ma,Yajie Feng,Junjie Ding,Youyu Duan,Xue Liu,Bin Zhang,Jinjing Tang,Xiaoping Tao,Liyong Gan,Shuangshuang Tan,Xiaoyuan Zhou
Rational fabricating advanced photocatalysts to recycle CO 2 into solar fuel is one of the most promising approaches to simultaneously address energy shortages and global warming. TiO 2 has been widely used in photocatalytic CO 2 reduction due to its abundant reserves, non-toxicity and harmlessness etc. However, it remains significantly challenging for practical applications due to the fast electron-hole recombination. Herein, we design a direct TiO 2 based Z-Scheme heterojunction by self-assembly of C-doped TiO 2 nanoparticles on 3D hierarchical petal of flower-like β-Bi 2 O 3 (C-TiO 2 /β-Bi 2 O 3 ). In situ electron paramagnetic resonance tests, in situ X-ray photoelectron spectroscopy and theoretical calculations reveal that electrons are transferred from β-Bi 2 O 3 to C-TiO 2 due to the formation of Z-Scheme heterojunction, which greatly accelerates the separation of photogenerated carriers to promote efficient CO 2 photoreduction. Ultimately, the optimal heterojunction shows significantly enhanced photocatalytic activity for CO 2 reduction to CO (31.07 μmol g –1 h −1 ) compared to the pure TiO 2 (3.28 μmol g –1 h −1 ) without using any photosensitizers and sacrificial agents. This work provides a unique insight to design direct Z-Scheme heterojunctions and delivers a promising photocatalyst for overcoming global warming and energy-supply issues.
研究领域:
材料科学
·
环境科学
·
化学工程
·
光催化
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DOI
高影响因子
Green Synthesis and Catalysis
·
2025
·
IF 6.3
Mengqian Song,Yu Li,Jinhui Feng,Shuhong Mao,Peiyuan Yao,Qiaqing Wu,Dunming Zhu
Chiral β-amino alcohols represent a significant structural motif in many biologically active molecules. Developing an efficient biocatalytic method for synthesizing chiral β-amino alcohols with structural diversity is highly desirable. In this study, enzymatic hydroxymethylation of aryl-aliphatic aldehydes by benzaldehyde lyases (BALs) enabled the synthesis of α-hydroxymethyl ketones at high substrate loadings (200 mmol/L - 300 mmol/L). And the reductive amination of diverse α-hydroxymethyl ketones by amine dehydrogenases (AmDHs) was conducted for synthesizing chiral β-amino alcohols in high isolated yields (60 %-79 %). Furthermore, a one-pot, two-step enzymatic process involving BAL-catalyzed hydroxymethylation of aldehydes and subsequent AmDH-catalyzed reductive amination was developed, enabling the synthesis of aromatic, aliphatic and aryl-aliphatic ( S )-β-amino alcohols from corresponding aldehydes in good to excellent isolated yields (56 %-96 %) and excellent enantioselectivity (>99 % ee ). The practical utility of this methodology was further demonstrated through the synthesis of key intermediates for antiviral drug Telaprevir or ATP competitive ERK inhibitor BVD-523.
研究领域:
有机化学
·
生物催化
·
酶学
·
药物化学
·
合成生物学
·
不对称合成
✓ 已验证
DOI
高影响因子
Frontiers in Bioengineering and Biotechnology
·
2025
·
IF 4.8
Zhanjia Zhang
Introduction: Lactose intolerance affects 85%–95% of Chinese adults, creating substantial demand for low-lactose dairy products. This study aimed to develop a cost-effective β-galactosidase production process through solid-state fermentation (SSF) using agricultural byproducts.Methods: A high-yielding strain was isolated from Tibetan fermented yak milk and identified through morphological, biochemical, and 16S rDNA sequence analysis. Solid-state fermentation conditions were optimized using single-factor experiments and Box-Behnken response surface methodology. Enzymatic properties were characterized, and the enzyme was applied to milk lactose hydrolysis. Techno-economic and environmental impact analyses were conducted.Results: Lactobacillus plantarum LP-15 exhibited initial enzyme activity of 44.7 U/g. Optimal SSF conditions were determined as substrate ratio of wheat bran:soybean meal:whey powder (6:3:1), 37 °C, pH 6.5, and 55% moisture content, achieving enzyme activity of 186.3 U/g (4.17-fold improvement). The enzyme exhibited optimal activity at pH 6.5 and 42 °C, with Km of 2.8 mM and catalytic efficiency of 5.3 × 104 M−1s−1. Milk lactose was reduced by 81.9% within 4 h using 2.0 U/mL enzyme at 40 °C, meeting the low-lactose standard (≤0.1%). SSF reduced production costs by 35.7%, water usage by 94%, and CO2 emissions by 62.4% compared to liquid fermentation.Discussion: This study provides an economically viable and environmentally sustainable solution for low-lactose dairy production in China, establishing independent intellectual property rights while addressing nutritional needs of lactose-intolerant populations through circular utilization of agricultural by products.
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产品关联
DOI
PubMed
高影响因子
FASEB JOURNAL
·
2022
·Vol. 36
·期 5
·e22266
·
IF 5.834
Huiqing Hu,Ruxing Zhao,Qin He,Chen Cui,Jia Song,Xinghong Guo,Nan Zang,Mengmeng Yang,Ying Zou,Jingwen Yang,Jinquan Li,Liming Wang,Longqing Xia,Lingshu Wang,Falian He,Xinguo Hou,Fei Yan,Li Chen
Type 2 diabetes mellitus (T2DM) is an age-related disease characterized by impaired pancreatic β cell function and insulin resistance. Recent studies have shown that the accumulation of senescent β cells under metabolic stress conditions leads to the progression of T2DM, while senolysis can improve the prognosis. However, the specific mechanism of β cell senescence is still unclear. In this study, we found that the increased load of senescence pancreatic β cells in both older mice and obese mice induced by high-fat diet (HFD) (DIO mice) was accompanied by activation of the Cyclic GMP-AMP synthase (cGAS) - stimulator of interferon genes (STING) pathway and using cGAS or STING small interfering RNA or STING inhibitor C176 to downregulate this pathway reduced the senescence-associated secretion profile (SASP) and senescence of Min6 cells treated with palmitic acid or hydrogen peroxide. C176 intervention in DIO mice also significantly reduced the inflammation and senescence of the islets, thereby protecting the function of pancreatic β cell and glucose metabolism. Our study further revealed that mitochondrial DNA (mtDNA) leakage under metabolic stress conditions was critical for the activation of the cGAS-STING pathway, which can be reversed by the mtDNA depleting agent ethidium bromide. Consistently, mtDNA leakage was more severe in older mice and was accelerated by a chronic HFD. In conclusion, we demonstrate that cytoplasmic mtDNA activates the cGAS-STING pathway to mediate SASP during the accelerated senescence of pancreatic β-cells induced by metabolic stress, and this process can be downregulated by the STING inhibitor C176.
研究领域:
内分泌学
·
细胞生物学
·
糖尿病研究
·
分子生物学
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DOI
PubMed
高影响因子
INORGANIC CHEMISTRY
·
2023
·
IF 4.6
Weizuo Li,Rui Fu,Jing Shi,Zhenhao Xiao,Yue Xu,Dafang He,Guangyu He,Haiqun Chen,Mingchen Xie
Through in-depth study of the properties and reaction mechanisms of catalysts, it is possible to better optimize catalytic systems and improve reaction efficiency and selectivity. This remains one of the challenges in the field of catalysis. Therefore, the research and design of catalysts play crucial roles in understanding and optimizing catalytic reaction mechanisms. A robust 2D zinc-based MOFs (Zn-HA) supported Co(II) ion catalyst (Zn-HA@Co) has been designed and synthesized via a coordination-assisted strategy for β-alkylation of secondary alcohols with primary alcohols. The characterization demonstrated that the anchoring of Co(II) on Zn-HA via coordination could efficiently enhance the Co(II) ion dispersity and interaction between Co(II) and Zn-HA MOFs. Importantly, the density functional theory results have provided mechanistic insights into the energy of the HOMO and LUMO of the Zn-HA@Co catalyst as well as the energy change of the entire process after interacting with the reactants and the specific energy changes of each orbital. The synthesized Zn-HA@Co MOFs effectively lower the energy barrier of the catalytic reaction process. We expect that our research and design of catalysts will serve as valuable guideline for understanding and optimizing catalytic reaction mechanisms.
研究领域:
材料化学
·
催化科学
·
理论化学
✓ 已验证
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Yutong Ye,Jinhao Zhang,Panpan Chen,Yongli Ji,Chunyan Ma,Jun Guo,Yi Liu,Hongli Chen
We investigated the mechanism by which the number of phenolic hydroxyl groups in flavonoids regulates their interactions with β-lactoglobulin in food systems. Kaempferol, quercetin, and myricetin were selected as model flavonoids with increasing number of hydroxyl groups. Spectroscopy revealed that increasing the hydroxyl content enhanced protein fluorescence quenching, promoted β-sheet formation, and induced a more compact protein conformation (39.7–42.6 %). Thermodynamic results demonstrated that hydrogen bonding dominated the binding process and that the binding affinity increased with hydroxyl number (binding constant: 9.55 to 36.31 × 10 6 L·mol −1 ). Kinetic analysis showed that a higher hydroxyl content reduced the activation energy (from 40.9 to 25.4 kJ·mol −1 ), allowing flavonoid–protein binding to be completed within 5 s. Flavonoid-induced protein compaction improved the emulsion stability and antioxidant performance, depending on hydroxyl number. These findings reveal a structure–interaction–function relationship and provide guidance for designing flavonoid–protein delivery systems in food applications.
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产品关联
DOI
PubMed
高影响因子
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