BIOMEDICAL CHROMATOGRAPHY
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2025
·Vol. 39
·期 7
·e70144
·
IF 1.7
Xu Fang,Ying Yang,Xinmei Cao,Yi Tao
Anoectochilus roxburghii (AR) has been reported to exhibit immunomodulatory activity. However, the underlying mechanism has not yet been fully elucidated. The present study employed a gas chromatography–mass spectrometry–based metabolomics approach, combined with histopathological and biochemical analyses, to investigate the metabolomic changes and therapeutic effects of AR in cyclophosphamide-induced immunosuppressed rats. The rats were divided into four groups: control group, model group, positive group, and AR-treated groups. The immunosuppressed model was established by intraperitoneal injection of cyclophosphamide (CTX, 150 mg/kg/day). The model group showed significant reductions in spleen and thymus indices, along with elevated levels of tumor necrosis factor-α (TNF-α) and interleukin-2 (IL-2) but decreased interleukin-10 (IL-10). AR treatment restored spleen and thymus indices and normalized TNF-α, IL-2, and IL-10 levels compared to the model group. Pattern recognition was employed to identify potential biomarkers. Pattern recognition analysis identified potential biomarkers: 26, 25, and 28 differential endogenous metabolites in the model, positive control, and AR-treated groups, respectively. Among these, 15 endogenous metabolites were validated using standard compounds, which demonstrated excellent linearity ( R 2 > 0.9991) and precision (intraday RSD < 4.82%, interday RSD < 4.39%). Recovery rates for the standards ranged from 95.40% to 104.91%, with RSD values below 5.97%. Pearson correlation analysis revealed that lactic acid, threonine, 5-oxoproline, palmitic acid, and arachidonic acid are positively correlated with spleen and thymus indices, whereas valine, urea, octaethylene glycol, and 2-palmitoyl glycerol are negatively correlated with these indices. Collectively, these findings demonstrate that AR extract enhances immunity in immunosuppressed rats by modulating lipid and amino acid metabolism.
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JOURNAL OF CHEMICAL AND ENGINEERING DATA
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2025
·
IF 2.1
Linlin Sun,Guodong Su,Zhenghui Li,Renjie Xu
The equilibrium mole fraction solubility of tanshinone I in acetone, N,N-dimethylformamide (DMF), methanol, ethanol, water, ethylene glycol (EG), n-propanol, isopropanol, and 1-butanol as well as methanol/ethanol/DMF/acetone + water was determined by the isothermal saturation method at 278.15–323.15 K under 101.2 kPa. The temperature and the composition of solvent mixtures play a decisive role in the solubility of tanshinone I. Then, four monosolvent models (Apelblat, van’t Hoff, λh, and NRTL) and three cosolvency models (Jouyban–Acree (J-A), van’t Hoff–Jouyban–Acree (V-J-A), and modified Apelblat–Jouyban–Acree (A-J-A)) were utilized to correlate the mole fraction of tanshinone I. The maximum deviation (relative average deviation (RAD) and root-mean-square deviation (RMSD)) between the experimental data and the solubility data calculated by the monosolvent models are 6.21% and 3.02 × 10–4, respectively. The maximum of RAD and RMSD for the mixture systems are 1.58% and 1.35 × 10–4, respectively. The Kamlet and Taft linear solvation energy relationship model was used to analyze the solvent effect, and the nonspecific dipolarity/polarizability interactions between the solute and solvent played a major promoting role. All in all, the experimental and computational data are able to provide basic data for the optimization and improvement of the recrystallization process of tanshinone I.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2025
·
IF 9.7
Ruyi Lu,Lishou Ban,Yihan Zhang,Jin Zhang,Xijun Liu,Jia He
The development of high-performance carbon-free Pt-based electrocatalysts that combine exceptional methanol oxidation activity with robust CO-poisoning resistance remained a significant challenge in fuel cell technology. Herein, we presented a new class of PtPdRuCuNi alloy aerogel electrocatalyst, featuring a structurally unique hollow network architecture with nanoporous ligaments (average diameter: 5.16 nm). The optimized catalyst exhibited remarkable mass activities of 2.05, 1.37, and 4.50 A mg Pt −1 in acidic, neutral, and alkaline methanol oxidation environments, respectively, surpassing the performance of commercial PtRu/C (0.74, 1.00, 2.06 A mg Pt −1 ). The CO oxidation peak potential exhibited a substantial 172 mV negative shift (0.352 V vs SCE) compared to conventional Pt/C (0.524 V). In-situ FTIR analysis uncovered a distinctive reaction pathway in which formic acid emerged as the primary intermediate during methanol oxidation processes at high potentials and successfully bypassed the conventional CO-mediated poisoning mechanism prevalent in Pt-based catalysts. When employed as an anode catalyst in DMFC, the alloy aerogel delivered a power density of 77.7 mW cm −2 at 65 °C, the mass-specific power attained a remarkable 109.7 mW mg Pt −1 at Pt loading of 0.37 mg cm −2 . This work introduced a new paradigm for carbon-free Pt-alloy catalyst design by leveraging alloy synergy and nanostructural engineering.
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Advanced Science
·
2025
·
IF 14.1
Tao Qin,Lian Qin,Yang Zhao,Yin Chen,Qianyue Liu,Xiaoguang Lin,Yongfeng Lan,Yaohui Huang,Yan Liu,Ke Zhang,Lifan Pan,Jiaxiao Li,Kunpeng Duan,Hao Liang,Mingjing Yin,Guiyang Fan,Lian Liu,Yu Deng,Lin Liao,Danke Su,Ming Gao,Junyu Lu
Sepsis induced acute liver injury (SALI), is a type of acute and severe disease that is generally characterized by producing significant amounts of reactive oxygen species (ROS) in liver tissue, and in response to excessive ROS, producing huge amounts of inflammatory factors by hepatocytes. Considering the crucial role of ROS in SALI, a Pd doped CeO 2 (CP) nano-heterojunction with enhanced ROS scavenging capacity is developed to act as a catalytic nanomedicine for the treatment of SALI. Combining with near infrared (NIR) irradiation, it exhibits excellent scavenging capacity of ROS including hydroxyl radical (•OH), superoxide anion (•O 2 − ), as well as singlet oxygen ( 1 O 2 ) for CP. Significantly, it also demonstrates the excellent antioxidant and anti-inflammatory activities for lipopolysaccharides (LPS) stimulated macrophages (RAW264.7), and cecum ligation and puncture (CLP) treated C57BL/6J mice via reducing intracellular ROS levels, decreasing inflammatory factors expression levels, as well as activating Keap1/Nrf-2/HO-1 pathway to reprogram redox homeostasis, induce cellular autophagy, reduce systemic inflammation and promote liver tissue repair, finally achieving the alleviation of SALI. It provides a promising therapeutic strategy of CP+NIR with high efficacy and biosafety for the management of SALI.
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Advanced Healthcare Materials
·
2025
·
IF 9.6
Yanxue Si,Ruitao Cha,Pai Zhang,Fengshan Zhou,Wenbing Yuan,Xingyu Jiang
5-Aminosalicylic acid (5-ASA) is the first-line drug for ulcerative colitis (UC), which encounters therapeutic constraints due to the poor water solubility and absorption by the upper gastrointestinal. Here, a cyclodextrin-based metal-organic framework (CD-MOF) is used as a carrier for 5-ASA (ASA@CM) to enhance its solubility. To improve the targeted release 5-ASA, ASA@CM is incorporated into sodium alginate/ L -arginine complex (SA-LA) to obtain the desired microspheres (ASA@CM/SL) by electrospray. ASA@CM/SL safeguards 5-ASA against the harsh gastrointestinal environment and provides favorable delivery to the colon. The anti-inflammatory and anti-oxidant activity is studied by measuring inflammatory factors and reactive oxygen species (ROS). The therapeutic effect of ASA@CM/SL on UC mice induced with dextran sulfate sodium is assessed. The biosafety by evaluating cytotoxicity and organ toxicity is studied. ASA@CM/SL shows excellent therapeutic effects for UC, as evidenced by its alleviation of inflammatory response and restoration of disrupted intestinal barriers. ASA@CM/SL features low cytotoxicity and low organ toxicity. This study provides a potential strategy for improving 5-ASA solubility and delivery efficiency, which may also be applied to broadly tackle various small hydrophobic molecules to treat UC and other intestinal diseases.
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JOURNAL OF FOOD AND DRUG ANALYSIS
·
2025
·Vol. 33
·期 2
·119
·
IF 3
Quan Liao,Zhenzhen Guo,Chenchun Ding,Renjie Zuo,Guoyan Liu
Chronic atrophic gastritis (CAG) is precancerosis with limited treatment options. This study investigated the therapeutic effects of velvet antler (VA), a traditional natural medicine, on CAG, with a focus on ferroptosis. In vivo experiments demonstrated the efficacy of VA in alleviating CAG, and network pharmacology analysis revealed its involvement in related terms, including inflammation, oxidative stress, and cell proliferation. Analyses further confirmed the modulation of ferroptosis by VA, particularly via the Keap1-Nrf2/HO-1/GPX4 pathway. In vitro assays supported these findings, demonstrating the ability of VA to reduce methylnitronitrosoguanidine-induced inflammation, oxidative stress, and ferroptosis in GES-1. This study provides a basis for exploring VA as a potential treatment for CAG, and offers new therapeutic strategies targeting ferroptosis.
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JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY
·
2025
·
IF 3.1
Li Quanyang,Yu Liang,Shen Lv,Fu Jiankun,Zhou Ru,Hao Min,Jiang Juncheng
Polypropylene (PP) is a widely used thermoplastic resin known for its strong properties, including chemical stability, electrical insulation, and high abrasion resistance. However, its low ignition point and rapid burning rate pose significant fire risks, necessitating the incorporation of flame retardants. In this study, cobalt metal–organic framework (Co-MOF), melamine polyphosphate (MPP), and expandable graphite (EG) were combined to enhance the flame retardancy of PP. The optimal blending ratio was determined, and the results showed that PP/MPP/2 mass% Co-MOF/15 mass% EG composites exhibited superior flame retardancy. These composites achieved a critical oxygen index (LOI) of 28%, a V-0 rating in the UL-94 test, and significantly reduced heat release and smoke production during combustion. The synergistic effect of MPP, Co-MOF, and EG facilitated the formation of a dense, thermally stable carbon layer, which effectively hindered flame propagation and improved fire safety. These findings provide valuable insights for the development of flame-retardant PP materials. Graphical abstract
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COMBUSTION AND FLAME
·
2025
·
IF 6.2
Nan Liu,Qi Chen,Shuang Yu,Zhiyuan Qian,Jie Chen,Xianwu Jiang
Methanol holds promise as a fuel for spark ignition engines in achieving carbon neutrality, though its cold-starting remains a key challenge. Non-equilibrium plasma excitation emerges as a transformative solution, where a mechanistic understanding of reaction kinetics, particularly electron-fuel interactions governing radical generation and chain branching, is pivotal to advancing the efficiency of plasma-assisted methanol ignition. This study investigates the kinetic interplay between non-equilibrium plasma excitation and methanol oxidation through combined experiments and modelling. A detailed kinetic mechanism for plasma-driven methanol ignition is developed, incorporating electron scattering cross-sections of CH 3 OH calculated via the R -matrix method. For the first time, wall quenching of electronically excited species is integrated into the model. Experimental validation using GC confirms the modelling accuracy in predicting species evolution. Key findings reveal that non-thermal plasma reduces ignition delay time by 1–3 orders of magnitude compared to auto-ignition, with 89 % of discharge energy allocated to non-equilibrium excitation and <10 % to thermal effects. Path flux analysis reveals that there are distinct dissociation pathways for methanol under plasma conditions. Combustion reactions predominantly produce CH 2 OH via H-atom abstraction, while plasma reactions primarily generate CH 3 O through e + CH 3 OH → e + CH 3 O + H and CH 3 OH + X ⁎ (N( 2 D), O( 1 D)) → CH 3 O + XH. The difference is due to the fact that excited CH 3 OH is dissociated into CH 3 O and H, demonstrated via Quantum chemical calculations. Notably, electronically excited methanol undergoes two competing processes: the wall quenching reactions (CH 3 OH(e) + Wall → CH 3 OH + Wall) and the dehydrogenation reactions (CH 3 OH(e) + OH/HO 2 → CH 2 OH + H 2 O/H 2 O 2 ). The former quenches excited species, while the latter increases fuel radicals, thereby accelerating the chain reaction. These insights bridge plasma physics and combustion chemistry, offering a predictive framework for optimizing plasma-assisted methanol ignition under cold-starting conditions.
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Gels
·
2025
·Vol. 11
·期 11
·852
·
IF 5.3
Minglin Wang,Ye Li,Kun Yang,Rui Liu,Mengqi Wang,Kongyin Zhao
The rapid development of industry has led to the discharge of large quantities of organic pollutants into water bodies, posing a significant threat to aquatic safety. It is imperative to develop efficient and environmentally friendly methods for the elimination of organic pollutants. The integration of hydrogel membranes with advanced oxidation processes (AOPs) for water purification has attracted considerable interest due to their high efficiency. However, conventional wet membrane materials stored in aqueous environments are more prone to swelling and leakage of loaded metal species. This limits its application in the degradation of organic pollutants. This study employs a vacuum drying strategy for wet hydrogels, incorporating molybdenum disulfide as a cocatalyst and Co2+cross-linking within the alginate matrix, resulting in a dried MoS2–cobalt alginate hydrogel membrane (D-MoS2-CoAlg). The drying process of the D-MoS2-CoAlg membrane not only significantly enhanced its mechanical strength and anti-swelling capacity but also effectively mitigated the leaching of Co2+. Throughout five consecutive cycles, the concentration of leached Co2+remained below 0.032 mg/L. This enables the membrane to achieve a balance between reusability and environmental compatibility. Under the conditions of a drying time of 60 min, a peroxymonosulfate (PMS) dosage of 0.2 mmol/L, and an initial methylisothiazolinone (MIT) concentration of 20 mg/L, the D-MoS2-CoAlg membrane exhibited exceptional catalytic performance, achieving a degradation rate of MIT as high as 92.14% within 5 min. The D-MoS2-CoAlg membrane demonstrates high catalytic activity and good stability, showing promising potential for application in the field of organic wastewater treatment.
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Sustainable Materials and Technologies
·
2025
·
IF 9.2
Zihao Chen,Xingqi Huang,Yanjun Zuo,Haibo Wang,Jingjing Zhang,Lingce Kong,Xiaoyan Lin
Peroxymonosulfate (PMS)-based advanced oxidation processes (AOPs) generate highly oxidizing radicals for efficient and eco-friendly degradation of organic pollutants. In this study, a composite catalyst of nitrogen-doped graphene (NGr) supported high-entropy alloy nanoparticles (MnFeCoCuZn, HEAs), denoted as HEAs/NGr, was synthesized via high-temperature calcination combined with metal salt impregnation. The synergistic effect of the high-entropy stability of HEAs and the NGr support minimized metal ion leaching (<0.0383 mg/L). The HEAs/NGr catalyst (100 mg/L) achieved 99.5 % degradation of Rhodamine B (RhB, 100 mg/L) within 5 min, with a rate constant of 1.0581 min −1 . The formation of M–N bonds between HEAs and NGr modulated the electronic structure of the composite, as evidenced by electrochemical tests showing enhanced interfacial electron transfer kinetics and PMS activation capability. Quenching experiments and EPR results confirmed that RhB degradation primarily followed a non-radical pathway dominated by singlet oxygen ( 1 O 2 ). The HEAs/NGr catalyst exhibited excellent tolerance to coexisting anions and maintained >94.3 % degradation efficiency across a pH range of 3.0–11.0. LC-MS and ECOSAR toxicity analysis confirmed effective RhB degradation and significantly reduced ecological toxicity of intermediates. This work deepens the understanding of multi-atomic synergy mechanisms and provides theoretical and practical insights for carbon-based water purification technologies.
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Food Science & Nutrition
·
2025
·Vol. 13
·期 11
·e71116
·
IF 3.8
Lanling Chu,Yuqi Dai,Qinghai Hu,Erzheng Su,Shuo Qi,Xiaoman Jiang,Anni Fu,Qianqian Jiang,Xuejun Kang
Traditional dietary assessment methods such as 24‐h dietary recall methods are not accurate enough, and the biomarkers to objectively evaluate food consumption have become attractive recently. Soy isoflavones are a typical plant‐based estrogen that helps to slow menopause symptoms, helping to lower cholesterol levels and reduce the risk of arteriosclerosis, and studies have shown a significant correlation between urinary isoflavones excretion and soy intake, which can be used as a useful biomarker. Because of the complex urine sample matrix and the large range of isoflavone concentration, it is vital to develop a new detection method to decrease the impurities interference and increase the target concentration for accurate detection of urine isoflavones. In this study, a few types of nanofibers were prepared and utilized as solid‐phase extraction adsorbents for sample pretreatment, and the homemade packed‐nanofiber solid‐phase extraction columns were chosen to purify the matrix and concentrate the analytes. A new method for rapid detection of soybean isoflavones in urine on the basis of packed‐fiber solid‐phase extraction combined with ultraviolet spectrophotometer was established, which provides an efficient reference for assessment of individual dietary intake. Electrospun nanofibers were prepared and utilized as solid‐phase extraction adsorbents for sample pretreatment. Homemade packed‐nanofiber solid‐phase extraction columns were created to purify the urine matrix and concentrate the analytes; that is, packed‐fiber solid‐phase extraction (PFSPE). A new method for rapid detection of the biomarkers of soybean isoflavones in urine on the basis of PFSPE combined with ultraviolet spectrophotometry was established, which provides an efficient reference for assessment of individual dietary intake.
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INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2025
·Vol. 26
·期 20
·10231
·
IF 4.9
Di Zhang,Lu Jiang,Yujiang Dai,Xinxin Si,Huifang Li,Komal Anjum
The bleeding sap of Luffa cylindrica (L.) Roem has been used for the treatment of tuberculosis since the record of Supplements to Compendium of Materia Medica . The active components and possible mechanism of it are yet ambiguous. Hence, this study is focused on investigating the possible mechanism underpinning this effect on the perspective of the antioxidant ingredients from the bleeding sap. Through organic solvents extraction, HPLC fractionation, DPPH trials evaluation, and UHPLC-Orbitrap tandem MS identification, a total of 37 compounds were identified from the bleeding sap with the strongest antioxidant ability. Network pharmacology, bioinformatics, and molecular docking as well as literature review revealed 13 compounds, including linoleic acid, abietic acid, and tretinoin, that might exert their anti-tuberculosis function via actions with PPARγ or MAPK pathway. These findings offer guidance for the potential applications of Luffa cylindrica (L.) Roem as a functional food.
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