International Journal of Nanomedicine
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2025
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IF 6.5
Lulu Zhang,Haiyang Liu,Jin Ren,Junyi Shao,Yanrong Cao,Shuangshuang Wang,Changchun Peng,Shengwen Shao,Haixia Ge,Jingmou Yu
Background Stimuli-responsive polymer-drug nanoparticles have emerged as a versatile and effective platform for delivering drugs in the treatment of malignant tumors. The integration of chemo-phototherapy has gained increasing prominence due to its remarkable synergistic antitumor effects.Methods Hydrophilic sericin (Ser) was linked to antitumor drug doxorubicin (DOX) via a disulfide bond. The obtained Ser-ss-DOX conjugate was characterized by Fourier-transform infrared spectroscopy (FTIR), proton nuclear magnetic resonance (1H NMR) and dynamic light scattering (DLS). The near-infrared (NIR) fluorescent dye IR780 was encapsulated into Ser-ss-DOX by a dialysis method. The physical state of IR780 within Ser-ss-DOX/IR780 nanoparticles was analyzed by differential scanning calorimetry (DSC). The photothermal properties and drug release behavior of IR780-loaded nanoparticles were systematically investigated. Cellular uptake and reactive oxygen species (ROS) generation capacity and in vitro cytotoxicity of IR780-containing nanoparticles were studied in 4T1 breast cancer cells. In addition, 3D multicellular tumor spheroid model was conducted to investigate the antitumor activity of the nanoparticles.Results The Ser-ss-DOX conjugate was successfully synthesized. Ser-ss-DOX and Ser-ss-DOX/IR780 nanoparticles were spherical in shape, and their particle sizes measured by DLS were 326 and 190 nm, respectively. IR780 was in an amorphous state within the Ser-ss-DOX/IR780 nanoparticles. The loading contents of DOX and IR780 were 8.26% and 2.78%, respectively. Ser-ss-DOX/IR780 exhibited ideal photothermal properties in vitro and reduction-sensitive drug release in a high glutathione (GSH) environment. Ser-ss-DOX/IR780 displayed higher cellular uptake in 4T1 cells than Ser-ss-DOX and free DOX. Under 808 nm laser irradiation, Ser-ss-DOX/IR780 showed a strong capacity to generate ROS. Importantly, Ser-ss-DOX/IR780 with laser irradiation effectively inhibited the growth of 4T1 cells and 3D multicellular tumor spheroids.Conclusion This work demonstrated that Ser-ss-DOX/IR780 integrated dual functionalities of chemotherapy and phototherapy, thereby enabling substantial potential for application in tumor treatment.
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CHIRALITY
·
2025
·Vol. 37
·期 11
·e70060
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IF 3
Shuling Dong,Jiayi Sun,Fan Ding,Ji Wu,Ronghua Fan,Li Zhou
In this work, a new strategy was proposed for the preparation of a chiral stationary phase based on β-cyclodextrin and calix[4]arene polymer. The chiral stationary phase was characterized by scanning electron microscopy, energy dispersive x-ray spectroscopy, Fourier transform infrared spectroscopy, and thermogravimetric analysis. The introduction of calix[4]arene polymer increased chirality recognition by increasing recognition sites and interactions, including hydrogen bonding, π–π interaction and synergistic inclusion effect. Compared with the home-made β-cyclodextrin–based chiral column, the prepared β-cyclodextrin–calix[4]arene polymer-based chiral column showed superior enantioseparation performance toward 1-phenylethanol, prothioconazole, promethazine, propranolol, and bisoprolol. These chiral compounds can be separated within 10 min under reversed-phase mode with resolutions ranging from 1.62 to 1.80. These findings will provide an important reference for developing novel supramolecule-based chiral stationary phases.
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Asian Journal of Pharmaceutical Sciences
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2025
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IF 11.9
Zeqian Huang,Congjun Xu,Yaqing Ding,Zishan Zeng,Huanxin Lin,Yong Luo,Xiaoyu Xu,Yanjuan Huang,Chunshun Zhao
Integrating photodynamic therapy (PDT) with immunosuppression reversal represents a promising synergistic approach to boost cancer immunotherapy. However, the complicated components and cumbersome preparation procedures of the currently developed nano drug delivery systems heavily hinder their further clinical translation. Herein, a reactive oxygen species (ROS)/photo dual-responsive amphipathic prodrug (denoted as PPTN) was designed and synthesized by linking NLG919, an indoleamine-2,3-dioxygenase (IDO) inhibitor, with the photosensitizer protoporphyrin IX (PpIX) by a thioketal moiety, and further modifying with mPEG 2k . PPTN could self-assemble into nanoscale unimolecular micelles in aqueous solution without additional excipients, increasing tumor accumulation while effectively addressing the pronounced hydrophobicity challenge of PpIX. Upon light exposure, PPTN generated ROS, not only directly damaging cancer cells, but also trigger the breakage of thioketal bond to accelerate simultaneous release of NLG919. Therefore, PPTN potentially act as a promising ROS/photo dual-responsive carrier-free prodrug delivery system for controllable drug release and specific tumor therapy. Moreover, PPTN induced simultaneous PDT-triggered immunogenic cell death (ICD) effect and specific IDO blockade to boost immune response, exhibiting potent suppression efficacy against primary and distant tumors. Overall, with the superiorities of easily controllable preparation procedures, synchronous drug delivery and ROS/photo dual-responsiveness, such a prodrug unimolecular micelle may represent a promising nanoplatform for photoactivated-immunotherapy.
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Journal of Environmental Chemical Engineering
·
2025
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IF 7.2
Guangyu Dong,Liushan Jiang,Linchun Nie,Jiawei Liao,Xiaodie Geng,Wenjing Liu,Dejing Meng,Meiling Xu,Na Liu,Qingxiang Zhou,Chunmao Chen
In this study, a magnetic covalent organic framework composite (Fe 3 O 4 @COF-BTCA-TMB) was conceived and synthesized with benzene-1,3,5-tricarbaldehyde and 3,3’,5,5’-tetramethyl benzidine as monomers. The modification of COF endowed the magnetic composite with a large number of functional groups and benzene rings, which exhibited high affinity to polycyclic aromatic hydrocarbons (PAHs) attributed to π-π conjugation and hydrophobic interaction. Based on these properties, it was utilized to conceive a highly efficient magnetic solid phase extraction method to enrich PAHs from water samples prior to high performance liquid chromatography analysis. The key factors that affect the extraction efficiencies of six PAHs in water samples were explored. Under the optimized conditions, six PAHs obtained good linearity in wide concentration ranges of 0.2–80 μg L −1 , 0.2–180 μg L −1 , and 0.2–240 μg L −1 , respectively. The limits of detection (LODs) ranged from 0.03 to 0.09 μg L −1 , and the enrichment factors were in the range of 107–149. The adsorption of PAHs on Fe 3 O 4 @COF was consistent with the quasi-second-order kinetic model, and Freundlich adsorption isotherm model. The conceived method was evaluated with real water samples and satisfactory recoveries (73.8 % ∼ 100.5 %) were obtained, indicating that it was simple, robust and efficient, and could be optioned for monitoring trace PAHs in environment.
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JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
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2024
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IF 4.5
Jingmou Yu,Qinglan Yuan,Chuan Li,Sile Hong,Yuting Li,Yafen Li,Jing Ren,Dengzhao Jiang,Pu Chen,Lei Zhang
The efficient drug release triggered by the tumor microenvironment is challenging in the development of stimuli-sensitive nanomedicine. In this study, we developed novel polymeric micelles sensitive to reactive oxygen species (ROS) for targeted delivery of doxorubicin (DOX) in tumor therapy. Hydrophilic chondroitin sulfate A (CSA) was conjugated with cholesteryl hemisuccinate (CHS) via a ROS-cleavable thioketal (TK) bond. The resulting CSA-TK-CHS could form self-assembled micelles, and DOX-loaded CSA-TK-CHS (CSA-TK-CHS/DOX) micelles displayed a nearly spherical shape with an average hydrodynamic diameter of 324 nm. The bioresponsive DOX release of CSA-TK-CHS/DOX micelles was evaluated in an H 2 O 2 -containing phosphate buffer solution (PBS). CSA-TK-CHS/DOX micelles showed higher cellular uptake than free DOX in 4T1 cells by confocal laser scanning microscopy (CLSM) observation. And CSA-TK-CHS/DOX improved DOX cytotoxicity against CD44-overexpressed 4T1 and CT26 tumor cells. Notably, CSA-TK-CHS/DOX micelles displayed significantly stronger potency ( P < 0.01) in antitumor activity than DOX·HCl in orthotropic 4T1-bearing Balb/c mice. Overall, these findings indicate that amphiphilic CSA-TK-CHS micelles are a promising targeted and intelligent drug carrier for tumor therapy.
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BIOMATERIALS
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2024
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IF 12.8
Di Ge,Siqi Ma,Tingting Sun,Yunfei Li,Jiaxing Wei,Chenao Wang,Xiaoyuan Chen,Yonghong Liao
Despite the recognized potential of inhaled nanomedicines to enhance and sustain local drug concentrations for lung cancer treatment, the influence of macrophage uptake on targeted nanoparticle delivery to and within tumors remains unclear. Here, we developed three ligand-coated nanoparticles for pulmonary delivery in lung cancer therapy: phenylboronic acid-modified nanoparticles (PBA-NPs), PBA combined with folic acid (FA-PBA-NPs), and PBA with mannose (MAN-PBA-NPs). In vitro, MAN-PBA-NPs were preferentially internalized by macrophages, whereas FA-PBA-NPs exhibited superior uptake by cancer cells compared to macrophages. Following intratracheal instillation into mice with orthotopic Lewis lung carcinoma tumors, all three nanoparticles showed similar lung retention. However, MAN-PBA-NPs were more prone to interception by lung macrophages, which limited their accumulation in tumor tissues. In contrast, both PBA-NPs and FA-PBA-NPs achieved comparable high tumor accumulation (∼11.3% of the dose). Furthermore, FA-PBA-NPs were internalized by ∼30% of cancer cells, significantly more than the 10–18% seen with PBA-NPs or MAN-PBA-NPs. Additionally, FA-PBA-NPs loaded with icaritin effectively inhibited the Wnt/β-catenin pathway, resulting in superior anti-tumor efficacy through targeted cancer cell delivery. Overall, FA-PBA-NPs demonstrated advantageous competitive uptake kinetics by cancer cells compared to macrophages, enhancing tumor targeting and therapeutic outcomes.
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FOOD CHEMISTRY
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2024
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IF 8.5
Changsheng Luo,Fang Xie,Qianqian Chen,Jiaojiao Zhou,Zhijun He,Jie Cai
Here, a facile self-assembly strategy was used to fabricate octenyl succinic anhydride starch (OSAS) nano micelles for encapsulation of K-carrageenan comprising curcumin (Cur) (KC/Cur-OSAS). KC/Cur-OSAS was used as a multipurpose edible food packaging coating on grapes. The characteristics, storage stability, photoactivated antibacterial properties and antimicrobial mechanisms of KC/Cur-OSAS were investigated. The experimental results demonstrated the potential photoactivated antibacterial performance of KC/Cur-OSAS gel coating, with an antibacterial efficacy of more than of 99 % against S. aureus and E. coli . Moreover, the coating showed good biocompatibility and 3D printing potential, and could reduce dewatering, prevent nutrient degradation, inhibit bacterial proliferation, increase nutritional content, maintain food quality, and extend the shelf life of food by more than 3 days, when conserved at a temperature of 25 °C. Hence, the light-driven KC/Cur-OSAS coatings prepared in this has great potential for improving nutrient delivery and extending the shelf life of perishable food and can be used for developing food packaging and preservation materials.
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ANALYTICA CHIMICA ACTA
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2024
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IF 5.7
Hui Bai,Lei Chen
Background As the derivatives of cyclodextrin (CD), cyclodextrin polymers (CDPs) effectively increase the concentration of CD units and construct supramolecular structures with unique stereoselectivity by the structure design. CDPs have shown significant potential in chiral separation, however, the process of stereoselective interactions on chiral stationary phases (CSPs) and the specific contribution of intermolecular forces are still a challenge issue. A comprehensive understanding of the chiral recognition mechanism of CDPs will help to optimize chiral separation conditions and design new CSPs. Results The star CDP with a supermolecular structure was synthesized by grafting β-CD onto the external 6-position hydroxyl groups using β-CD as the parent nucleus. The enhanced host-guest recognition ability of CD supramolecular polymer structure provided better inclusion interaction and increased chiral recognition of the isomers. The Star-CD CSP with star CDP as a chiral ligand performed satisfactory stereoisomer separation ability with the separation factor (α) up to 2.0 for various quinoline alkaloid isomers and 1.89 for catechins. To elucidate its chiral separation mechanism, molecular docking was used to construct the three-dimensional visual models of the binding sites and the contribution of non-covalent interactions between Star-CD CSP and quinoline alkaloid isomers. In addition, the formation sites of non-covalent interactions on the CD monomers of the polymer side chains were confirmed from the actual geometric structure by analyzing the NMR chemical shift changes before and after the formation of complexes between Star-CD polymers and isomers. Combined with the mutual evidence of molecular simulation and chiral NMR, the specific recognition mechanism of selector-selectand complexes was comprehensively expounded. Significance The multi-mode CSP based on cyclodextrin supramolecular structure provides new ideas for the stereoisomeric separation of complex chiral components with multiple chiral centers in natural products. Not limited to the macroscopic performance of the chromatographic separation, molecular docking explored the theoretical model of chiral recognition from the molecular level. The chiral NMR analysis confirmed the credibility of the model from the geometry structure, and then the recognition mechanism of multi-mode CSP was fully elaborated combining the above three aspects.
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CHEMICAL ENGINEERING JOURNAL
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2025
·
IF 13.2
Rong Chen,Xiangrong Dai,Rongjiao Sun,Yi Liao,Jiajia Dong,Ying Tan,Xuru Jin
Acute Lung Injury (ALI) is a clinical syndrome associated with various respiratory diseases and injuries. While inhalable drug delivery systems offer the advantage of direct pulmonary targeting and reduced systemic toxicity, their therapeutic efficacy is frequently compromised by the tracheobronchial mucus barrier. To address this limitation, we developed PCL-ss-PSBMA, a novel nanocarrier engineered to overcome the constraints of conventional micelle platforms, which typically depend on either hydrophilic coatings or single-response mechanisms. By integrating zwitterionic groups and disulfide bonds in a dual-functional design, PCL-ss-PSBMA achieves synergistic optimization of mucus barrier penetration and glutathione (GSH)-triggered drug release. In addition, the nanocarrier also exhibits excellent biocompatibility, further ensuring its safety in therapeutic applications. In an ALI murine model, PCL-ss-PSBMA@DEX markedly improved the delivery efficiency and utilization rate of dexamethasone (DEX) through its dual mechanisms of mucus penetration and stimulus-responsive drug release. This design not only enhances the deposition of the drug in the lungs but also achieves efficient treatment of ALI by precisely controlling drug release, demonstrating its potential as an advanced strategy for treating respiratory diseases.
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Nature Communications
·
2024
·Vol. 15
·期 1
·1-10
·
IF 14.7
Chu Fengjian,Zhao Gaosheng,Wei Wei,Shuaibu Nazifi Sani,Feng Hongru,Pan Yuanjiang,Wang Xiaozhi
Although numerous ambient ionization mass spectroscopy technologies have been developed over the past 20 years to address diverse analytical circumstances, a single-ion source technique that can handle all analyte types is still lacking. Here, a wide-energy programmable microwave plasma-ionization mass spectrometry (WPMPI-MS) system is presented, through which MS analysis can achieve high coverage of substances with various characteristics by digitally regulating the microwave energy. In addition, ionization energy can be rapidly scanned using programmable waveforms, enabling the simultaneous detection of biomolecules, heavy metals, non-polar molecules, etc., in seconds. WPMPI-MS performs well in analyzing real samples, rapidly analyzing nine toxicological standards in one drop of serum, and demonstrating good quantification and liquid chromatography coupling capability. The WPMPI-MS has also been used to detect soil extracts, solid pharmaceuticals, and landfill leachate, further demonstrating its robust analytical capabilities for real samples. The prospective uses of the technology in biological and chemical analysis are extensive, and it is anticipated to emerge as a viable alternative to commercially available ion sources.
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Small
·
2025
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IF 12.1
Xu Wang,Yong Yan,Huan Zhou,Xinyu Liu,Riyi Zhang,Dong Wang,Peter Schaaf,Guangsheng Guo,Xiayan Wang
Polycyclic aromatic hydrocarbons (PAHs) and organophosphorus pesticides (OPPs) are typical pollutants characterized by a wide variety of types, difficulty in degradation, and significant differences in mutagenic and carcinogenic toxicity. However, due to their distinct physicochemical properties, it remains challenging to simultaneously analyze PAHs and OPPs under real environmental conditions within the same simplified detection systems and accurately assess their oxidation-reduction processes. In this study, electrochemical surface-enhanced Raman scattering (EC-SERS), utilizing a microfluidic chip of self-made 3D nanoporous Au loaded with Ag nanoparticles, is employed to analyze PAHs and OPPs in the environment and investigate their oxidation-reduction processes, which displayed excellent sensitivity and selectivity. The chip successfully analyzed three types of PAHs and four types of OPPs, achieving the lowest detection limit of 0.01 ppb, and strong linearity within a certain range. The recovery in real water samples reached 102.89% with a relative standard deviation (RSD) of 13.36%. Furthermore, this system successfully identified different types of pollutants and their concentrations in mixed samples with high accuracy. Meanwhile, preliminary insights into the electrochemical oxidation and degradation processes of these pollutants are provided, offering a first understanding of their transformation mechanisms.
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JOURNAL OF HAZARDOUS MATERIALS
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2025
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IF 11.3
Yibin Yang,Rui Chen,Zhiqiang Zhang,Yuke Qin,Attiqa Parveen,He Bai,Xiaoqiang Jia
Phenanthrene, a pervasive polycyclic aromatic hydrocarbon (PAH), poses significant environmental and health risks due to its persistence and bioaccumulation. This study presents the development of a synthetic bacterial consortium comprising three engineered Escherichia coli BL21(DE3) strains, each harboring distinct catabolic modules encoded by pET-28(a) plasmids for the complete mineralization of phenanthrene. The consortium was engineered by integrating 17 genes critical for phenanthrene degradation, organized into three distinct modules that harness the collective metabolic potential of separate strains to enhance the degradation process. Each module was tailored to a specific phase of phenanthrene catabolism, ensuring the coordinated and efficient breakdown of the pollutant. Following the identification of rate-limiting enzymes within the up- and downstream degradation modules, the eight corresponding genes were subjected to laboratory evolution using the SCRaMbLE method to bolster the degradation capabilities of the strains. The consortium was then tested in a minimal salt medium (MSM) containing 100 mg/L phenanthrene. Following their evolution, the two SCRaMbLE strains E. coli SCRPHE033 and E. coli SCRKA065 exhibited enhanced degradation rates of 11.5 and 25.18 % for their respective substrates. Finally, the comprehensive phenanthrene degradation system, which includes the two SCRaMbLE strains and E. coli CRSA, was subjected to comprehensive optimization, after which it achieved a significant degradation efficiency of 84.33 % for 100 mg/L phenanthrene within a 120-hour timeframe. This research demonstrates the potential of synthetic biology in constructing microbial consortia for environmental bioremediation based on the SCRaMbLE method, offering a promising strategy for mitigating PAH pollution.
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