Journal of Advanced Research
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2026
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IF 17.1
Xueting Liu,Chang Yan,Ruiqi Su,Mengwen Shi,Zhi Geng,Ying Chen,Liwen Zhu,Zongjie Dai,Junfeng Hui,Xi Chen,Kai-Zhi Jia
Introduction Pyridoxal-5′-phosphate (PLP), the ubiquitous and ancient cofactor, plays important roles in enzymatic elimination, transamination and other reactions. The catalytic efficiency of PLP-dependent enzymes is significantly higher than that of free PLP. The recruitment of solution PLP by the enzymes, particularly through interactions outside the active-site entrance, is the key step determining the occurrence of PLP-mediated catalysis. However, the precise mechanism by which enzymes recruit solution PLP remains elusive. Objectives This study aims to elucidate the mechanism by which enzymes, specifically methionine γ-lyase (MGL) that suppresses cancer cell proliferation through serum or dietary methionine depletion, recruit solution PLP and to investigate the role of the C-terminal domain in this process. The study also seeks to explore the relationship between PLP recruitment and the efficient L-methionine catabolism in the host organism. Methods We report the crystal structure of yMGL, which belongs to a newly identified subgroup of cystathionine γ-lyases, in complex with L-methionine and PLP. To investigate the functional role of the C-terminal domain in PLP recruitment and enzyme activity, we performed C-terminal domain truncations and mutations and assessed their effects both in vitro and in vivo , analyzing changes in PLP binding affinity and L-methionine catabolism, respectively. Results Through structural, biochemical, bioinformatic and metabolic analyses, we demonstrate that the C-terminal domain of yMGL, outside the canonical PLP-binding domain, is essential for the specific interaction between yMGL and PLP, and L-methionine catabolism. A conserved Ser residue within this domain, located outside of active-site entrance, determines PLP recruitment. Conclusion These findings elucidate a previously uncharacterized mechanism of PLP recruitment by MGLs and enable rational design of MGLs with enhanced PLP binding and catalytic performance.
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POLYMER DEGRADATION AND STABILITY
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2026
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IF 8.1
Shanshan Lin,Ge Yang,Xiaolin Zhao,Hailing Guo,Hao Wu,Ning Zheng,Svetlana Mintova,Zhibo Li
Polyethylene terephthalate (PET) glycolysis using heterogeneous catalysts is often limited by the poor accessibility of polymer chains to catalytic active sites, particularly in microporous systems. Herein, undercoordinated Zn Lewis acid sites were constructed on the external surface of ZSM-5 through controlled Zn modification, while strong interactions between Zn species and framework-associated Al enhance both Lewis acidity and catalyst stability. The resulting accessible Lewis acid sites facilitate polymer-catalyst contact, enabling complete PET conversion within 30 min under mild glycolysis conditions. The catalyst exhibited a high Zn utilization efficiency of 24.7 g PET ·g Zn -1 ·min -1 , maintained stable catalytic performance over 10 cycles with negligible Zn loss, and remained effective for post-consumer PET feedstocks. This work demonstrates that engineering the spatial distribution and coordination environment of Lewis acid sites is an effective strategy for heterogeneous polymer depolymerization.
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Food Bioscience
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2026
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IF 6.2
Jiawen Lu,Ying Yang,Xingtao Zhou,Qiang Yu,Jianhua Xie,Yi Chen
Acrylamide (AA) and 5-hydroxymethylfurfural (HMF) are common food processing hazards frequently co-existing in thermally processed foods. Although both hazards have been reported to induce oxidative stress and cytotoxicity, their co-exposure effects on intestinal epithelial cells remain unclear. Here, we investigated the co-exposure effects and mechanisms of AA and HMF in intestinal epithelial IEC-6 cells. Co-exposure significantly reduced cell viability, disrupted redox homeostasis, and impaired mitochondrial function, as evidenced by glutathione depletion, increased lipid peroxidation, and mitochondrial membrane potential loss. Multiple lines of evidence supported the involvement of ferroptosis, including glutathione peroxidase 4 (GPX4) downregulation, intracellular iron accumulation, lipid reactive oxygen species (ROS) generation, and the protective effect of ferrostatin-1. Transcriptomic analysis further provided supportive evidence for ferroptosis-related pathways, which was further supported by decreased SLC7A11 expression. In parallel, alterations in autophagy-related markers and lysosomal function were observed, and chloroquine partially attenuated cellular injury, suggesting that impaired autophagic degradation may contribute to the cellular response to AA and HMF exposure. In addition, co-exposure induced nuclear accumulation of Nrf2 together with increased HO-1 expression, indicating activation of an adaptive antioxidant response to oxidative stress. Collectively, AA and HMF co-exposure induced intestinal epithelial injury associated with ferroptosis, accompanied by autophagy-lysosome dysfunction and activation of the Nrf2/HO-1 signaling. These findings improve our understanding of the cellular mechanisms underlying AA and HMF co-exposure in intestinal epithelial cells.
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POSTHARVEST BIOLOGY AND TECHNOLOGY
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2026
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IF 7.3
Renli Ai,Kai Yan,Changyu Shao,Longfeng Wei,Qinqin He,Junxian Chen,Qinyi Hu,Yao Wang,Zhu Li
The exploration of effective biocontrol agents and the characterization of their functional components are crucial for precisely preventing and managing carrot postharvest disease. Herein, Aspergillus niger xj was shown to effectively inhibit the bacterial soft-rot pathogen named Pectobacterium aroidearum , with the functional compounds primarily localized in the extracellular fermentation broth. Correlation analysis between the inhibitory activity and content of 5-hydroxymethyl-2-furancarboxylic acid (HMFCA) across 224 atmospheric and room-temperature plasma mutant strains and original strain xj indicated that HMFCA was an important inhibitory agent. The bioactivity of the bio-derived HMFCA isolated from the positive mutant strain 350–28 was validated through assays. In vitro inhibitory assays revealed that the half-maximal effective concentration (EC 50 ) of HMFCA against P. aroidearum was 1121 ± 8 μg mL −1 , with a minimum inhibitory concentration of 1.25 mg mL −1 and a minimum bactericidal concentration of 2.5 mg mL −1 . Moreover, HMFCA exhibited considerable potential to impede the growth of P. aroidearum . Furthermore, in vivo inhibitory assays indicated that HMFCA could assist carrot in resisting the infection caused by P. aroidearum within 24 h at three concentrations: EC 20 , EC 50 , and EC 80. However, the required effective concentration markedly increased at 48 h. These findings indicated that A. niger exhibited biocontrol activity through the extracellular secretion of active secondary metabolites, which directly suppressed the growth and reproduction of P. aroidearum . In particular, HMFCA was a functional compound, which offered a promising approach for the targeted postharvest management of carrot.
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JOURNAL OF MOLECULAR STRUCTURE
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2026
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IF 4.7
Meiling Nie,Ting Jiao,Lin Wang,Chiyi Wang,Jiahan Jin,Jialu Hu,Yang Li,Hailu Zhang
The study of pharmaceutical cocrystal polymorphism is attracting increasing interest, as variations in intermolecular interactions and packing arrangements between an active pharmaceutical ingredient (API) and the same cocrystal former (CCF) directly impact the extent to which cocrystals can tailor the API’s properties. However, the number of reported cases of cocrystal polymorphism remains relatively limited. Herein, we propose that orientational variability of hydrogen bond donors or acceptors on either the API or the CCF may drive the formation of cocrystal polymorphs. To substantiate this hypothesis, 5-fluorocytosine (5-FC) and L -malic acid ( L -MAL) were selected as the model cocrystallization system, and two distinct 2:1 polymorphs (form Ⅰ and form Ⅱ) were ultimately identified. Crystal structural analysis revealed that the two cocrystals share consistent supramolecular interactions; however, the divergent orientations of carbonyl and hydroxyl groups in L -MAL induce distinguishable hydrogen bonding configurations and molecular stacking patterns, which strongly validates the proposed origin of cocrystal polymorphism. Lattice energy calculations, polymorphic transformation studies, and melting point data collectively confirmed that these two polymorphs exhibit a monotropic thermodynamic relationship, with the high- Z ’ form Ⅱ ( Z ’ = 2) being the thermodynamically stable crystalline phase. Under higher relative humidity condition (90 % RH), an obvious phase transformation of form Ⅰ was observed within one week, whereas form Ⅱ maintains its phase stability for at least four weeks. Such performance difference between these two cocrystals further underscores the practical significance of cocrystal polymorph screening.
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Canadian Journal of Chemistry
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2024
·Vol. 102
·期 9
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IF 1.1
Yuanyuan Zhang,Mengyi Ba,Wen Li,Yanli Song,Xiang Xu,Haixin Liu,Wenxuan Zhao,Tao Sun,Zhiqiang Cai
Herein, we synthesized and used a novel pillar[5]arene with two functionalizable rims (AP5A-C10-OAc) as stationary phase in gas chromatography (GC). The AP5A-C10-OAc column (0.25 mm, i.d.) showed a high column efficiency of 4107 plates/m and medium polarity. The AP5A-C10-OAc stationary phase possesses unique amphiphilic structure, including a π-electron rich cavity and two functionalized arms composed of five nonpolar alkyl chains and five polar ester chains, and showed good separation performance for diverse types of analytes and isomers (apolar/polar, aliphatic/aromatic). Particularly, the AP5A-C10-OAc phase showed high selectivity for the challenging isomers of chloronitrobenzenes, bromonitrobenzenes, benzenediols, and iodoanilines with distinct advantages if compared with the commercial polysiloxane column.
研究领域:
分析化学
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色谱科学
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ACS Applied Materials & Interfaces
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2022
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IF 10.383
Weijiong Dai,Lina Zhang,Runze Liu,Guangjun Wu,Naijia Guan,Landong Li
Zeolites with good acid site accessibility and high diffusion rates are highly desirable catalysts, especially when dealing with bulk molecules. In this work, ZSM-5 zeolites with similar Si/Al ratios but different thicknesses along the b-axis (from ∼30 nm to ∼5 μm), namely, two plate-like ZSM-5 zeolites and two reference zeolites have been prepared and the impacts of b-axis thickness on the surface properties and catalytic cracking performances are explored. Comprehensive physiochemical studies demonstrate that reducing the b-axis thickness of the zeolite crystals endows the samples with better acid site accessibility and more external surface acid sites. Two model compounds with different molecule sizes, namely, 1,3,5-triisopropylbenzene (TIPB) and cumene, are selected to explore the catalytic cracking performances of the as-synthesized samples. The results reveal that decreasing the b-axis thickness of zeolite crystals can effectually promote the catalytic activity and stability in catalytic cracking reactions. For TIPB cracking, the greatly enhanced catalytic activity is ascribed to the enhanced acid site accessibility in plate-like ZSM-5 zeolites, and for cumene cracking, the improved catalytic stability is ascribed to the shortened diffusion length of plate-like zeolites.
研究领域:
催化化学
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材料科学
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化学工程
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石油化工
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纳米多孔材料
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Canadian Journal of Chemistry
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2022
·Vol. 100
·期 8
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IF 1.051
Changshuai Liu,Rong Zhang,Rujing Pei,Chao Zhang,Fuxiang Li
A new strategy was implemented to synthesize hierarchical ZSM-5 zeolite, which uses self-made organosilanized double-terminal amino polyetheramine (ODAP) as a soft template to generate mesopores. The influence of the additional amount of ODAP on the textural properties and morphologies of the hierarchical ZSM-5 zeolite was investigated. The obtained hierarchical ZSM-5 zeolite was characterized by X-ray diffraction, N2 physical adsorption, SEM, temperature-programmed desorption of NH3, and other methods. The analysis of the experimental results shows that the maximum total BET specific surface area and mesoporous specific surface area of the optimized hierarchical ZSM-5 zeolite reached 609 and 278 m2/g, respectively. Because the synthesized hierarchical ZSM-5 zeolite has a large mesoporous specific surface area and abundant surface acidity, in terms of reactant conversion and product selectivity, compared with microporous zeolite, it exhibits excellent catalytic performance in the thiophene alkylation desulfurization reaction. This synthesis strategy can be extended to the preparation of zeolites with different framework structures, which is of great significance.
研究领域:
材料科学
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催化化学
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无机化学
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多孔材料
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CHEMICAL ENGINEERING JOURNAL
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2026
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IF 13.2
Wenting Cai,Shuai Liu,Tongxue Zhang,Ke Fan,Xubin Zhang,Fumin Wang,Jing-Jing Lv,Xijun Liu
The electrooxidation of 5-hydroxymethylfurfural (HMF) into 2,5-furandicarboxylic acid (FDCA) serves as an effective means to substitute the kinetically slow anode oxygen evolution reaction (OER) in water electrolysis for hydrogen generation. Nickel-based materials are promising catalysts, but the weak adsorption ability of the active species NiOOH on HMF leads to slow dehydrogenation kinetics, making the rational design of active Ni sites a huge challenge. Herein, a novel heterojunction catalyst (NiO@Co 3 S 4 /NF) with S vacancies is successfully synthesized by interface engineering. Specifically, the construction of the heterostructure optimizes the electronic structure of Ni, which in turn facilitates the generation of NiOOH and enhances its ability to adsorb substrate molecules. S vacancies are beneficial for exposing more active sites and increasing the active surface area. Density functional theory calculations demonstrate that the interfacial electronic redistribution effectively adjusted the d-band center of Ni, and the strong d-p orbital coupling interaction between Ni and HMF can significantly lower the energy barrier of the dehydrogenation step, thereby promoting the highly selective oxidation of HMF to FDCA. Consequently, the NiO@Co 3 S 4 /NF exhibits outstanding electrocatalytic activity, achieving a current density of 50 mA cm −2 at only 1.30 V vs. RHE, with a FDCA yield of 99.62% and a Faradaic efficiency of 99.67%, along with excellent cycling stability. Our work provides new insights into catalyst regulation for biomass upgrading.
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BIOORGANIC CHEMISTRY
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2026
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IF 4.7
Jinnian Wang,Haoming Zhao,Shijun Tong,Xingjian Zhang,Shuyu Ding,Han Zhang,Zhong-Hua Yang
The industrial production of pyridoxal 5′-phosphate (PLP)—an indispensable enzymatic cofactor—is hampered by inefficient multistep chemical syntheses and the economic infeasibility of ATP-dependent biocatalytic routes. To address these challenges, we report an ATP-autonomous biocatalytic cascade that couples pyridoxal kinase (PLK) with polyphosphate kinase (PPK) for sustainable PLP synthesis. This system harnesses inexpensive sodium hexametaphosphate (SHMP) as a phosphoryl donor to drive continuous ATP regeneration, thereby enabling efficient phosphorylation of pyridoxal to PLP without exogenous ATP input. Recombinant PLK and PPK were co-expressed in E. coli BL21(DE3), yielding specific activities of 50 and 145 U/g cell , respectively. The whole-cell biocatalyst achieved only about 25% conversion yield due to mass transfer limitations and intracellular PLP degradation. Notably, while the optimized cell-free system (45 °C, pH 6.5, PLK:PPK = 1:3, 25 mM SHMP) attained a remarkable 95% PLP yield. This 3.8-fold enhancement demonstrates the critical advantage of eliminating cellular barriers in complex multi-enzyme cascades. Our work establishes a robust, ATP-independent platform for high-efficiency PLP biosynthesis that seamlessly integrates cofactor regeneration with cascade catalysis, offering a scalable and economically viable route for industrial biomanufacturing. Moreover, it demonstrates the broader utility of polyphosphate-driven energy recycling in sustainable biocatalysis.
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PubMed
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CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
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2026
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IF 3
Sheng Zhizheng,Zhou Jian,Ye Zhaoqi,Wang Tingting,Wang Weihua,Wang Yangdong,Teng Jiawei,Xie Zaiku
Mass transfer on surface of nano-zeolites plays an important impact on their catalytic performance. In this work, we investigated a diffusion-controlled strategy to regulate the interface effect and the dynamic behavior of guest molecules at the interface of nanoZSM-5 via chemical deposition of different silicon (Si) or tungsten (W) species, thereby affecting its performance in methanol to propylene (MTP) reaction. It was verified that only about 1% of sedimentary species form stable surface structures by binding with surface hydroxyl groups and defect hydroxyl groups. Under a pressure of 0.2–5 kPa, Si and W modification respectively increased the surface diffusion efficiency of methanol by 50% and decreased it by 60%, demonstrating the bidirectionality of the control strategy. Meanwhile, acidity and structural characterization confirmed that these properties were not strongly affected. Catalytic results showed that surface diffusion enhancement increased the selectivity of ethylene and propylene, and remained stable within 120 h. Mechanism studies have shown that the dynamics of the accumulated surface species is a key intermediate process that connects surface diffusion and catalytic performance.
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PHOSPHORUS SULFUR AND SILICON AND THE RELATED ELEMENTS
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2020
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IF 1.046
Daoke Yu,Wenbin Chen,Shichao Zhang,Rakesh Chilivery,Fuhai Li,Xupeng Lu,Yibing Song,Yiwen Fang
The synthesis conditions of HZSM-5 zeolite, including crystallization temperature, crystallization time and raw material ratio, were investigated by L32 (48) orthogonal test to specifically optimize its performance in dimethyl ether (DME) aromatization for the first time. Based on the total yield of aromatic products, the synthesis conditions of HZSM-5 zeolite with the best DME aromatization properties were obtained by comprehensive analysis and validation experiments. The relationship between the aromatization performance, crystalline structure, pore structure, and acidity of HZSM-5 zeolite were analyzed. The results showed that the HZSM-5 zeolite accompanied by hierarchical structure, an appropriate Brønsted and Lewis acid content and uniform crystal morphology, was successfully synthesized under optimized conditions. Over this unmodified and un-doped catalyst, the conversion of DME approached to 99.3% and the total yield of aromatics reached was 53.5%.
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