JOURNAL OF ETHNOPHARMACOLOGY
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2024
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IF 4.8
Sihao Zhang,Danmei Tian,Zixuan Xia,Fengge Yang,Yanhui Chen,Zhihong Yao,Yi He,Xinglong Miao,Guirong Zhou,Xinsheng Yao,Jinshan Tang
Ethnopharmacological relevance Chang-Kang-Fang (CKF), originated from traditional Chinese medicine (TCM) formulas, has been utilized to treat diarrhea predominant irritable bowel syndrome (IBS-D) based on clinical experience. However, the underlying mechanism of CKF for treating IBS-D remains unclear and need further clarification. Aim of the study The objective of this present investigation was to validate the efficacy of CKF on IBS-D model rats and to uncover its potential mechanism for the treatment of IBS-D. Materials and Methods We first established the IBS-D rat model through neonatal maternal separation (NMS) in combination with restraint stress (RS) and the administration of senna decoction via gavage. To confirm the therapeutic effect of CKF on treating IBS-D, abdominal withdrawal reflex (AWR) scores, the quantity of fecal pellets, and the fecal water content (FWC) were measured to evaluate the influence of CKF on visceral hypersensitivity and the severity of diarrhea symptom after the intragastric administration of CKF for 14 days. Subsequently, enzyme linked immunosorbent assay (ELISA) was applied to assess the effect of CKF on neuropeptides substance P (SP) and 5-hydroxytryptamine (5-HT), as well as inflammatory cytokines in serum and in intestinal tissues. Further, colonic pathological changes, the amount of colonic mast cells, and the expression level of occludin in rat colon tissues, were investigated by hematoxylin-eosin (HE) staining, toluidine blue staining, and immunohistochemistry, respectively. To explore the underlying mechanisms, alterations in colonic RNA transcriptomics for the normal, model, and CKF treatment groups were assessed using RNA sequencing (RNA-Seq). Subsequently, quantitative real-time polymerase chain reaction (qRT-PCR), western blot (WB), and immunofluorescence (IF) assays were applied to validate the effect of CKF on predicted pathways in vivo and in vitro . In addition, to elucidate the potential active compounds in CKF, 11 representative components found in CKF were selected, and their anti-inflammation potentials were evaluated using LPS-treated RAW264.7 cell models. Results CKF treatment significantly reduced the number of fecal pellets, attenuated visceral hypersensitivity, and decreased 5-HT and SP concentrations in serum and colon tissues, along with a reduction in colonic mast cell counts, correlating with improved symptoms in IBS-D rats. Meanwhile, CKF treatment reduced the colonic inflammatory cell infiltration, lowered the levels of IL-6, TNF- α , and IL-1 β in serum and colon tissues, and increased the occludin protein expression in colon tissues to improve inflammatory response and colonic barrier function. RNA-Seq, in conjugation with our previous network pharmacology analysis, indicated that CKF might mitigate the symptoms of IBS-D rats by inhibiting the Toll like receptor 4/Nuclear factor kappa-B/NLR family pyrin domain-containing protein 3 (TLR4/NF- κ B/NLRP3) pathway, which was confirmed by WB, IF, and qRT-PCR experiments in vivo and in vitro . Furthermore, coptisine, berberine, hyperoside, epicatechin, and gallic acid present in CKF emerged as potential active components for treating IBS-D, as they demonstrated in vitro anti-inflammatory effects. Conclusion Our findings demonstrate that CKF effectively improves the symptoms of IBS-D rats, potentially through the inhibition of the TLR4/NF- κ B/NLRP3 pathway. Moreover, this study unveils the potential bioactive components in CKF that could be applied in the treatment of IBS-D.
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ACS Biomaterials Science & Engineering
·
2025
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IF 5.5
Yang Zhang,Han Wang,Ke Liu,Ruimeng Sun,Yurou Wang,Jiayu Guo,Wenxiang Zhou,Haoran Zheng,Yanfei Qi
Acute renal injury (AKI) has a high incidence rate and mortality, but current treatment methods are limited. As a kind of nanomaterial with enzyme-like activity, nanozyme has shown outstanding advantages in treating AKI according to recent reports. Herein, we assess the potential of manganese-based nanozymes (MnO2–BSA NPs) with excellent biosafety in effectively alleviating AKI. Our findings in vitro and in vivo reveal that MnO2–BSA NPs exert regulatory effects on oxidative stress, inflammation, and apoptosis. These effects are mediated through activation of the Nrf2/HO-1 and PI3K/Akt/NF-κB pathways. Notably, it was observed that the cytoprotective effect of MnO2–BSA NPs is abrogated upon inhibition of Nrf2 expression, highlighting the important role of this transcription factor in cellular protection. In summary, the study demonstrates the protective effect of MnO2–BSA NPs in AKI and provides the molecular mechanisms involved, which can contribute to the advancement of potential therapeutic interventions for nanozyme-based treatments.
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INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
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2024
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IF 8.1
Shucan Qin,Qianqian Peng,Weizhen Kong,Rong Xue,Chongqing Xu,Jianming Shi,Yanrong Liu,Yunqian Ma
Integrating sulfide oxidation reaction (SOR) with hydrogen evolution reaction (HER) by electrochemical means to simultaneously valorize sulfion and produce hydrogen is an efficient cleaner production method. Currently, we reported on the fabrication of one succulent-like phosphorus-doped transition metal selenide bifunctional catalyst supported on nickel foam (P–CoSe/NF) for SOR and HER. The unique structure has a significant surface area that allows many active sites to be exposed, enabling the catalyst to have an excellent activity up to 100 mA cm −2 at only 0.362 V. The H 2 Faraday efficiency reaches 97.4% and the rate of sulfur evolution is 26.24 mg h −1 . The durability test demonstrates that the bifunctional catalyst possesses excellent anti-sulfur properties. The Density Functional Theory calculations demonstrate that phosphorus as a dopant is believed to confer an optimal electronic structure and local coordination environment by tuning d electron number. The calculate S 8 ∗ to S 8 was confirmed to have a low energy barrier, indicating the self-cleaning tendency caused by phosphorus doping. This work presents a promising approach utilizing anti-sulfur single-atom-doped transition metal selenide catalysts in electrochemical reaction, representing a significant advancement in achieving low-cost and high value-added using waste resources.
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SKIN RESEARCH AND TECHNOLOGY
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2024
·
IF 2
Guiyu an Chen,Yingxun Chen,Yan Zhang,Shufeng Zheng,Louying Zhu,Mingxing Ding
Background Incontinence‐associated dermatitis (IAD) is a tough problem in clinical settings, not only increasing the risk of complications like catheter‐related urinary tract infections and pressure ulcers in elderly and critically ill patients, but also prolonging hospital stays, raising hospital costs, and possibly leading to medical disputes. This study is aimed to evaluate the therapeutic effect of silicone dressing combined with topical oxygen therapy on IAD in a rat model. Methods An IAD rat model induced by synthetic urine with trypsin was established. Hematoxylin & eosin staining was carried out to examine skin histology. Using immunofluorescence, the microvessel density in the affected skin tissues was determined. ELISA was performed to measure the concentrations of inflammatory cytokines and angiogenic factors in serum. The mRNA expression of EGF, PDGF, and VEGF was detected via qRT‐PCR. Western blotting was employed to determine NF‐κB p65/STAT1 pathway‐related protein levels. Results Compared to single therapy, silicone dressing combined with topical oxygen therapy could significantly reduce the severity of IAD, improve skin histology, inhibit inflammation, and promote angiogenesis in IAD rat models. Additionally, the results showed that relatively speaking, the combined therapy suppressed the NF‐κB p65/STAT1 signaling pathway more effectively. Conclusion These findings indicated that silicone dressing combined with topical oxygen therapy can alleviate IAD through promoting wound healing and inhibiting inflammation via NF‐κB p65/STAT1 signaling pathway in a rat model, which provided a theoretical basis for the prevention and treatment of IAD in clinic.
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SEPARATION AND PURIFICATION TECHNOLOGY
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2025
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IF 9
Haorui Wang,Zhiqiang Zhang,Jiaye Liu,Lihong Wang,Qingliang Liu,Tao Zhang
In the coal chemical industry, direct evaporation of reverse osmosis (RO) concentrate generates numerous hazardous solid wastes containing mixed salts and organic matters. Nanofiltration (NF) membrane can selectively separate these contents and reduce the disposal of solid waste. However, the high concentration of organics in the RO concentrate exacerbates NF membrane fouling. We applied O 3 and O 3 /H 2 O 2 as pretreatment methods to mitigate the membrane fouling. The excitation-emission matrix (EEM) fluorescence spectroscopy demonstrated that O 3 /H 2 O 2 was more effective at removing organic matter in RO concentrate. Organic molecular weight distribution specifically showed that O 3 /H 2 O 2 was more efficient at degrading large organic molecules, making it more effective in reducing membrane fouling. The strong oxidative ability of the O 3 /H 2 O 2 system allowed the potential mineralization of smaller organics. In contrast, the O 3 oxidation increased the concentration of organic matter with small molecular weights ( M w < 1,000 Da). The thorough degradation of organics using O 3 /H 2 O 2 reduced the organic content in the produced mixed salts.
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INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
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2024
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IF 8.1
Jiejie Feng,Changshun Chu,Jianting Liu,Liling Wei,Huayi Li,Jianquan Shen
To push forward the hydrogen energy technology, it is crucial to develop a highly active catalyst for the application of water splitting. Nevertheless, many electrocatalysts suffer from functional limitations, making them unsuitable for both anode and cathode applications. Therefore, the utilization of bifunctional electrocatalysts is necessary to overcome this limitation. In this study, we have reported a bifunctional CoMoP-Fe x P/NF catalyst with favorable electrocatalytic performance toward hydrogen evolution reaction/oxygen evolution reaction (HER/OER). Balanced amorphous/crystalline heterophase endows CoMoP-Fe x P/NF with more exposed active sites. In addition, the superhydrophobic nature of CoMoP-Fe x P/NF helps to improve the contact with electrolyte. Following conditional optimization, CoMoP-Fe x P/NF requires only an overpotential of 85.6 mV to achieve a current density of 10 mA cm −2 for HER, and 249.6 mV for a current density of 20 mA cm −2 for OER. A water-splitting electrolyzer comprising CoMoP-Fe x P/NF requires a low cell voltage of 1.56 V to achieve a current density of 10 mA cm −2 . This study provides valuable insights into designing bifunctional electrocatalysts and replacing noble metallic materials to enhance hydrogen production from water splitting.
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DESALINATION
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2024
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IF 8.3
Xuewu Zhu,Feiyue Ge,Xinwei Kang,Liping Qiu,Bin Liu,Jingtao Xu,Feihong Wang,Daoji Wu,Zhe Yang,Daliang Xu,Heng Liang
Nanofiltration membranes (NF) possessing high flux, excellent selectivity, and chlorine resistance are urgently required for healthy drinking water treatment. In this work, we prepared a high-performance polyesteramide (PEA) nanofiltration membrane featuring polyester and polyamide composite structures using piperazine (PIP) and polyvinyl alcohol (PVA) as co -monomers via sodium hydroxide catalyzing interfacial polymerization. The resultant NF membranes' surface morphology, chemical structure, filtration performance, and chlorine stability were systematically evaluated. The optimized PEA membrane (PEA-TFC) possessed a terraced morphology and relatively high roughness (23.9 nm). During purifying real surface water, a high flux (20.29 LMH bar −1 ) with <50 % mineral rejection and over 70 % organic matter removal was achieved for PEA-TFC, demonstrating excellent organic/mineral selectivity. In addition, the polyamide and polyester structures provided uniform pore size distribution and fewer chlorine-active sites, granting PEA-TFC excellent chlorine resistance and stability. The exceptional organic/mineral selective separations and remarkable chlorine resistance endow the PEA-TFC as a promising candidate for healthy drinking water NF.
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Nutrients
·
2024
·Vol. 16
·期 22
·3854
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IF 4.8
Yannan Zhang,Sijuan Huang,Shuai Zhang,Zhihui Hao,Jianzhong Shen
Background: Diarrhea-predominant irritable bowel syndrome (IBS-D) is a common chronic functional gastrointestinal disorder that causes diarrheal and intestinal barrier disruptions. Although pomegranate peel extract (PPE) has been reported for the treatment of diarrheal and intestinal inflammation, its effectiveness and mechanisms specifically for the treatment of IBS-D remain unknown. Objectives: This study aimed to explore the therapeutic effect of PPE on IBS-D and elucidate its underlying mechanisms. Methods: A high-fat diet, restraint stress, and senna gavage were combined to establish a rat model mimicking IBS-D, to evaluate the therapeutic effects of PPE. Network pharmacology analysis, serum medicinal chemistry, and transcriptomics were employed to investigate potential downstream signaling pathways. Findings were further validated through molecular docking and Western blot analysis. Results: The findings revealed that PPE significantly improved the symptoms of IBS-D, ameliorated intestinal inflammation, and promoted intestinal barrier function. The target genes in the MAPK and NF-κB signaling pathways were significantly enriched and down-regulated. Molecular docking and Western blot assays were performed to verify that PPE had a high affinity for the protein candidates in these pathways, and significantly down-regulated the expression of p-IκB, p-p65, p-JNK, p-p38, and p-ERK1/2. Conclusions: The present study is the first to demonstrate that PPE alleviates diarrheal and intestinal damage in IBS-D, potentially by inhibiting MAPK and NF-κB signaling pathways. These findings suggest that PPE may provide a novel therapeutic option for IBS-D.
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ENVIRONMENTAL POLLUTION
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2025
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IF 7.3
Zixu Li,Yacan Gong,Emmanuel Sunday Okeke,Dan Li,Yao Chen,Weiwei Feng,Ting Zhao,Liuqing Yang,Guanghua Mao,Xiangyang Wu
Typical plasticizer dibutyl phthalate (DBP) has been demonstrated to induce hepatotoxicity in zebrafish, but the underlying molecular mechanisms remain incompletely elucidated. Numerous studies have shown that ferroptosis is involved in the pathophysiological progression of hepatic disease. However, it remains unclear whether ferroptosis is involved in the DBP-induced hepatotoxicity in zebrafish. Initially, histopathological analyses have preliminarily confirmed that DBP can activate inflammatory responses in the zebrafish liver. Further investigation revealed that DBP induces ferroptosis in the zebrafish liver, characterized by iron overload, lipid peroxidation, and aberrant activation of ferroptosis pathways. Furthermore, DBP exposure induced ferroptosis, disrupting cell membranes and subsequent release of HMGB1, which are sensed by immunocytes TLR4/NF-κB signaling pathways, thereby activating the innate immune response in a context-dependent manner. Moreover, the ferroptosis inhibitor Fer-1 effectively rescues the activation of HMGB1-TLR4/NF-κB-mediated immune processes. Overall, this work enriches the molecular mechanism of DBP-induced zebrafish liver inflammatory damage and provides a reliable biomarker for future environmental risk assessment of DBP.
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Catalysts
·
2025
·Vol. 15
·期 8
·725
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IF 4
Bingxian Zhu,Yachao Liu,Yue Yan,Hui Wang,Yu Zhang,Ying Xin,Weijuan Xu,Qingshan Zhao
Hydrogen energy is a pivotal carrier for achieving carbon neutrality, requiring green and efficient production via water electrolysis. However, the anodic oxygen evolution reaction (OER) involves a sluggish four-electron transfer process, resulting in high overpotentials, while the prohibitive cost and complex preparation of precious metal catalysts impede large-scale commercialization. In this study, we develop a FeCo-based bimetallic deep eutectic solvent (FeCo-DES) as a multifunctional reaction medium for engineering a three-dimensional (3D) coral-like FeOOH-Co2(OH)3Cl/NF composite via a mild one-step impregnation approach (70 °C, ambient pressure). The FeCo-DES simultaneously serves as the solvent, metal source, and redox agent, driving the controlled in situ assembly of FeOOH-Co2(OH)3Cl hybrids on Ni(OH)2/NiOOH-coated nickel foam (NF). This hierarchical architecture induces synergistic enhancement through geometric structural effects combined with multi-component electronic interactions. Consequently, the FeOOH-Co2(OH)3Cl/NF catalyst achieves a remarkably low overpotential of 197 mV at 100 mA cm−2and a Tafel slope of 65.9 mV dec−1, along with 98% current retention over 24 h chronopotentiometry. This study pioneers a DES-mediated strategy for designing robust composite catalysts, establishing a scalable blueprint for high-performance and low-cost OER systems.
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CHEMICAL ENGINEERING JOURNAL
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2025
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IF 13.2
Ziyi Wang,Yuqian Zhang,Chengping Li,Liang Wang,Ting Bian,Shitan Yan,Kuzmin Anton,Liuting Zhang
Incorporating solar steam generation with the hydrovoltaic effect presents a promising approach to concurrently addressing water scarcity and energy challenges. However, realizing a high water evaporation rate while simultaneously maintaining substantial electrical output in a single device remains a formidable obstacle. Here, this study presents a novel hydrovoltaic and galvanic effects-coupled evaporation platform (HGEP) for dual-function applications in freshwater production and electricity generation from seawater. Coating urchin-like Mn-doped Co 3 O 4 on inclined nickel foam (NF), the system achieves a stable evaporation rate of 1.77 kg m −2 h −1 in 3.5 wt% brine under 1 kW m −2 irradiation with a 30° tilt angle. The inclined structure facilitates unidirectional ion transport by establishing a salinity gradient, effectively preventing salt deposition for up to 8 h. Moreover, integrating Zr-Al electrodes effectively combines the hydrovoltaic and galvanic effects, enabling the HGEP to deliver a stable open-circuit voltage of 1.0 V and a short-circuit current of 1.5 mA—over an order of magnitude higher than the pure hydrovoltaic effect (0.49 V, 0.15 mA)—and achieve a maximum power density of 0.8 W m −2 . This study presents an innovative solar-powered water treatment technology that simultaneously achieves water production and power generation
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CHEMICO-BIOLOGICAL INTERACTIONS
·
2025
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IF 5.4
Yuting Guan,Jinghua Ruan,Pingping Tan,Songwei Qian,Size Zhou,Ao Zhang,Yuchong Fu,Shuhui Zhao,Yuqing Ran,Xing Feng,Yijia Wang,Xinlei Wu,Bing Zhang,Weiping Ji,Lianpin Wu,Xiaoling Guo
Kawasaki Disease (KD) is an acute and self-limiting vasculitis of unknown etiology that mainly occurs in infancy and can lead to vascular endothelial injury. Hesperidin (HES) is an economical dietary biological flavonoid with anti-oxidant, anti-inflammatory, and anti-apoptotic pharmacological effects. The main objective of this study was to investigate the protective effects of HES on KD, and try to elucidate the underlying mechanism. The Candida albicans water-soluble fraction (CAWS) was used to induce coronary arteritis of KD mouse model in vivo , and tumor necrosis factor α (TNF-α) was employed to induce human umbilical vein endothelial cell (HUVEC) injury of KD cell model in vitro to investigate the anti-inflammatory and anti-apoptotic effects of HES on KD. Our in vivo results showed that HES significantly reduced coronary artery injury in KD mice by alleviating pericoronary inflammatory infiltration and tissue fibrosis, inhibiting inflammatory cytokines and chemokine expressions, and decreasing vascular endothelial cell apoptosis. Our in vitro study confirmed that HES had the opposite ability of the NF-κB agonist NF-ĸB activator 1 (ACT1) to significantly alleviate the inflammatory response, CellROX level, and apoptosis by decreasing BAX/BCL-2 and Cleaved Caspase-3 levels as well as reducing TUNEL positive cells and the ratio of flow cytometric apoptotic cells in TNF-α induced HUVECs. The further mechanism study based on bioinformatics analysis and western blotting demonstrated that HES could protect against vascular inflammation and cell apoptosis of KD through inhibiting the TLR4/IĸBα/NF-ĸB pathway, suggesting that HES may be a promising therapeutic candidate for KD.
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