JOURNAL OF MATERIALS SCIENCE
·
2018
·Vol. 53
·期 9
·6366-6377
·
IF 2.993
Li Wenqi,Ding Xingeng,Meng Cheng,Ren Chunrong,Wu Huating,Yang Hui
Rare earth phosphates (REPO 4 ) are considered as promising materials to immobilize the high-level radioactive waste. In the paper, Gd 1− x Yb x PO 4 ( x = 0, 0.1, 0.2, 0.4, 0.6, 0.8 and 1.0) series were prepared via the solid-state reaction using Gd as the surrogate for minor actinide Am and Cm, and the structure, phase transformation and morphology were characterized by XRD, Raman, TEM and SEM. With the calcination temperature ranging from 1200 to 1500 °C, the coexisted phases of monazite- and xenotime-type structure are observed in Gd 0.9 Yb 0.1 PO 4 . The monophasic xenotime-type Gd 0.9 Yb 0.1 PO 4 is obtained at 1600 °C. The monazite → xenotime transformation of Gd 1− x Yb x PO 4 depends on the substitution of Gd 3+ by Yb 3+ ions. With the decrease in Gd 3+ content, the gradual changes of FWHM and the hypsochromic shift of P-O symmetrical stretching vibration (Vs) are observed in the Raman spectra, indicating that the distortion of PO 4 tetrahedra occurs during the phase transformation process. The chemical durability test is measured by the ASTM product consistency test leaching method, and the normalized mass loss (NL Gd ) of Gd and (NL Yb ) of Yb is extremely low and shown in the order of 10 −6 –10 −7 g/m 2 for all the ceramics.
研究领域:
材料科学
·
核废料管理
·
固态化学
·
辐射化学
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DOI
高影响因子
CHEMOSPHERE
·
2018
·
IF 4.427
Peng Liu,Xiangyu Wang,Jun Ma,Huiling Liu,Ping Ning
To provide superior substrates and determine the specific species of immobilized nano zero-valent iron (NZVI) system, polyacrylonitrile (PAN) membrane was functionalized by bio-inspired polydopamine (PDA) and poly( l -DOPA) (PDOPA) for efficient immobilization of NZVI. The synthesized composites were denoted as PAN/PDA-NZVI (PPN) and PAN/PDOPA-NZVI (PON), respectively. Analyses of XRD, SEM/EDS and XPS show that the aggregation and release of iron nanoparticles had been successfully controlled by improving membrane hydrophilcity and iron-chelating capacity via the graft of functionalized groups (i.e. OH and COOH) of PDA and PDOPA on PAN membrane. Both PPN and PON composites exhibited superior reactivity for Cr(VI) removal (Cr(VI) removal efficiency and reaction rate were 2.21–2.22 and 9.90–10.14 times higher than that of bare NZVI, respectively). The stability and recyclability of PPN and PON composites could be maintained over repeated cycles. Further analyses indicate that PON is more capable for Cr(VI) elimination than PPN due to the proprietary carboxyl of l -DOPA. With the addition of 1,10-phenanthroline, membrane-chelated Fe(II) was determined to be the major species in Cr(VI) removal system, accounting for 56.9% and 53.8% with regard to PPN and PON composites, and Fe 0 was responsible for the reduction of residual Cr(VI). Analyse of reacted composites revealed that Cr(VI) was completely converted into Cr(III), followed by formation of dominant Cr(III)/Fe(III) (oxy)hydroxides and partial desorption from NZVI reactive sites. This study suggested that both synthesized PPN and PON composites have potentials for Cr(VI)-contaminated wastewater treatment .
研究领域:
环境科学
·
材料科学
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DOI
PubMed
高影响因子
BRAZILIAN JOURNAL OF BIOLOGY
·
2021
·
IF 1.651
Huang Y.,Zhang N.-J.,Zhao Z.
As an important enzyme, xylanase is widely used in the food, pulp, and textile industry. Different applications of xylanase warrant specific conditions including temperature and pH. This study aimed to carry out sodium alginate beads as carrier to immobilize previous reported mutated xylanase from Neocallimastix patriciarum which expressed in E. coli, the activity of immobilization of mutated xylanase was elevated about 4% at pH 6 and 13% at 62 °C. Moreover, the immobilized mutated xylanase retained a greater proportion of its activity than the wide type in thermostability. These properties suggested that the immobilization of mutated xylanase has potential to apply in biobleaching industry.
研究领域:
酶工程
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工业微生物学
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生物技术
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DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2022
·
IF 11.889
Dingsheng Wu,Pengfei Lv,Quan Feng,Yu Jiang,Hanrui Yang,Mensah Alfred,Qufu Wei
Laccase is a promising biocatalyst for pollutant degradation and water purification. However, laccase can only improve the stability of enzyme activity and achieve its significant catalytic effect after effective immobilization. Herein, we report a general strategy to integrate nanocellulose aerogel and laccase for high-efficiency degradation of organic pollutants. Biomass-derived functional bacterial cellulose (BC) aerogel with a nanonetwork structure and high porosity was prepared by biosynthesis, solvent replacement, and atom transfer radical polymerization (ATRP) procedures. Subsequently, a biocatalyst platform was fabricated by “coupling” ATRP-modified BC aerogel with abundant active sites with laccase through ion coordination. The results demonstrated the biocatalyst platform not only has good biological affinity, but also has high enzyme load and structural stability. Meanwhile, the degradation rates of reactive red X-3B and 2, 4-dichlorophenol reached 94.5% and 85.2% within 4 h, respectively. The strategy disclosed herein could provide a practical method for the degradation of organic pollutants.
研究领域:
环境科学
·
生物技术
·
材料科学
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DOI
高影响因子
International Journal of Environmental Science and Technology
·
2020
·Vol. 17
·期 9
·4061-4074
·
IF 2.54
Liu H.,Li D.,Shao L.,Wu D.,Zhang H.,Yuan J.,Xu F.
In this study, a crack-free PDMS–SiO 2 –BiPO 4 (P/Si/Bi) composite was prepared via a solgel-assisted route by dispersing pre-prepared bismuth oxybromide into a solution that was composed of hydroxyl-terminated polydimethylsiloxane (PDMS–OH), tetraethyl orthosilicate and phosphoric acid. Analysis via the FTIR, XRD, XPS and SEM–EDX techniques demonstrated that the nonisolated BiPO 4 particles with a monoclinic crystal phase were uniformly distributed on the surface of the P/Si/Bi composite, which can provide more active sites and a larger surface area for photocatalysis compared with other materials. The photocatalytic activity of the P/Si/Bi composite loaded with various mass ratios of BiPO 4 (9 wt%, 12 wt%, 18 wt% and 27 wt%) was investigated via the degradation of methylene blue (MB, 10 mg/L) under UV light irradiation. It was found that the P/Si/Bi composite loaded with 18 wt% BiPO 4 realized the highest degradation efficiency of MB, and its corresponding apparent rate constant was approximately 2.2 times that of the PDMS–SiO 2 –TiO 2 composite (loaded with 18 wt% TiO 2 ). The effects of inorganic ions on the degradation of MB were also investigated. The static contact angle and photocatalytic activity of the P/Si/Bi composite changed minimally after five cyclic experiments, thereby demonstrating its remarkable durability in sustainable applications. In addition, the photogenerated reactive species that were responsible for the degradation of MB within the P/Si/Bi composite were identified, and a photocatalytic mechanism was proposed.
研究领域:
材料科学
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环境科学
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DOI
高影响因子
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
·
2015
·Vol. 20
·期 5
·901-907
·
IF 1.113
Yang Jianbing,Ni Kefeng,Wei Dongzhi,Ren Yuhong
Ni 2+ -functionalized Fe 3 O 4 @polydopamine magnetic nanoparticles (Ni 2+ -PD-MNPs) were designed and synthesized by in situ coating of magnetic nanoparticles with polydopamine, followed by conjugation of Ni 2+ to the polydopamine film. The Ni 2+ -PD-MNPs were used to purify His-tagged red fluorescent protein (His-RFP) via affinity interaction between Ni 2+ and the His-tag. The results showed that the Ni 2+ -PD-MNPs had extraordinary selectivity for His-RFP purification. In addition, a Histagged transaminase (ω-transaminase BJ110) was selectively immobilized onto the Ni 2+ -PD-MNPs without purification, and the immobilized enzyme showed improved specific activity, as well as enhanced stability and reusability.
研究领域:
生物化学
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纳米生物学
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蛋白质工程
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DOI
高影响因子
CELLULOSE
·
2021
·Vol. 28
·期 10
·6287-6303
·
IF 5.044
Ma Zhengxin,Liu Jie,Shen Guixian,Zheng Xuejing,Pei Ying,Tang Keyong
Abstract Synthesis of nanocomposites containing silver nanoparticles (AgNPs) has drawn growing interest owing to their antimicrobial activity and tuneable physicochemical properties. In this study, we report the surface modification of microfibrillated cellulose (MFC) with bio-inspired polydopamine (PDA) followed by in-situ growth and immobilization of AgNPs. The resultant AgNPs-PDA-MFC nanocomposites were characterized by UV–Vis spectrophotometry, scanning electron microscopy, energy dispersive X-ray spectroscopy, transmission electron microscopy, and X-ray diffraction. The results showed that crystalline face-centered cubic AgNPs with a mean diameter of 19.1 nm were randomly and firmly immobilized on MFC. The attenuated total reflection Fourier transform infrared analysis results confirmed the formation of AgNPs, and the content of silver in AgNPs-PDA-MFC was determined by thermogravimetry. The aqueous suspension of AgNPs-PDA-MFC was stable during 30 days of storage. Antibacterial activity of the AgNPs-PDA-MFC nanocomposites was evaluated with Escherichia coli and Staphylococcus aureus. Importantly, AgNPs-PDA-MFC exhibited excellent long-term antibacterial activity. This was ascribed to the extremely slow but sustained release of silver from the AgNPs-PDA-MFC (0.56% in 14 days). Furthermore, the application of AgNPs-PDA-MFC as coating and filling agents was preliminarily evaluated. This study suggests that the AgNPs-PDA-MFC nanocomposites with long-term antibacterial performance provide a promising solution for fighting against pathogenic bacteria. Graphic abstract
研究领域:
材料科学
·
微生物学
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DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2022
·
IF 8.008
Hao Zhu,Xiangli Li,Zhimei He,Yun Chen,Jun-Jie Zhu
The biomineralized metal–organic frameworks (MOFs) as protective layers help enhance the robustness of enzymes for biocatalysis. Despite great efforts, it is still challenging to develop a recyclable system with high payload and tolerance to harsh conditions. Here, we report a facile surface charge-independent strategy based on Zn-based coordination polymer (ZnCP) for nondestructive immobilization of enzyme. The ZnCP outcompetes most of the previously reported MOFs, in terms of high-payload enzyme packaging. Moreover, benefiting from the hydrophilicity of ZnCP, the entrapped enzymes (e.g., positive cytochrome C and negative glucose oxidase) maintained high catalytic activity, resembling their native counterparts. Notably, compared with ZIF-8, such enzyme-incorporated ZnCP (enzyme@ZnCP) is more tolerant to acidic pH, which imparts the enzyme with good recyclability, even in acid species-generated catalytic reactions, thus broadening its application in biocatalysis. The feasibility of enzyme@ZnCP for protein packaging, enzyme cascade catalysis, and biosensing was also validated. Altogether, enzyme@ZnCP demonstrates high enzyme payload, operational stability, and preservation of enzymatic activity, affording a versatile platform to accommodate bioactive enzyme for biocatalysis and biosensing.
研究领域:
材料科学
·
生物化学
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生物技术
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化学工程
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DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2021
·
IF 9.147
Peng Wang,Cen Zhang,Yucheng Zou,Yang Li,Hui Zhang
In this study, lysozyme and dextran sulfate sodium salt (DS) were alternately deposited on the surface of electrospun cellulose acetate (CA) nanofibers, which were then heated at 55 °C for 6 days to induce crosslinking via Maillard reaction . The non-heated self-assembled nanofibers presented the increased average fiber diameter from 397 to 501 nm with increasing number of layers, but an improved thermal stability was induced by Maillard reaction due to the formation of covalent bonds. After heating, the immobilized lysozyme lost less than 40% of its initial activity, but exhibited the enhanced temperature tolerance and storage stability, and retained over 55.6% of its original activity after 12 reuses. Moreover, the heated nanofibrous mats showed substantially higher antibacterial activities against Staphylococcus aureus and Escherichia coli , compared to the non-heated ones. These results suggested the promising applications of the post-covalent layer-by-layer (LBL) nanofibrous mats in improving enzyme performance in food , pharmaceutical or personal care products.
研究领域:
材料科学
·
生物材料
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生物技术
·
食品科学
·
药学
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DOI
高影响因子
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2026
·
IF 3.5
Wei Guoguang,Zhou Ning,Wei Jie,Zhang Alei,Chen Kequan,He Hao
Cadaverine, an essential precursor for bio-based nylon PA5X production, has emerged as a highly valuable compound in the engineered plastics and synthetic fibers industry. Current industrial biomanufacturing of cadaverine depends critically on the enzymatic activity of L-lysine decarboxylase (LDC). In this investigation, we systematically evaluated ten LDC variants originating from six distinct bacterial species, all cloned and expressed in Escherichia coli BL21(DE3). Comprehensive characterization identified Sp LDC from Serratia proteamaculans as the most promising candidate, demonstrating exceptional catalytic activity under alkaline conditions. Enzymatic analysis showed that Sp LDC established optimal activity at pH 6.5 and 52 °C, with remarkable stability maintained between pH 5.5-8.0 and temperatures of 37–52 °C during prolonged 12-hour incubations. Kinetic studies revealed favorable catalytic parameters ( K m = 11.5 mM, V max = 1000 U/mg), indicating efficient substrate conversion. To enhance industrial applicability, we developed an immobilized enzyme system by genetically fusing Sp LDC with a chitin-binding domain (ChBD) and subsequently immobilizing it on chitin supports. This engineered biocatalyst demonstrated superior performance compared to whole-cell systems, achieving a 3.4-fold enhancement in cadaverine production efficiency. The immobilized preparation exhibited excellent operational stability, retaining over 50% of its initial activity through ten repeated reaction cycles. This work establishes an efficient, stable, and reusable enzymatic platform for cadaverine biosynthesis, offering significant potential for sustainable nylon PA5X production at industrial scales.
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DOI
高影响因子
JOURNAL OF THE AMERICAN CERAMIC SOCIETY
·
2026
·Vol. 109
·期 6
·e70882
·
IF 4.2
Jinchun Wei,Mingfen Wen,Wenhong Han,Xi Peng,Jiaqin Wei,Xiaoyan Shu,Zhanqiang Li,Xirui Lu
Simple, rapid, and efficient sintering is a crucial step in the process of high temperature composites fabrication, and the fast reaction mechanism is of great scientific significance. For the first time, this study employs laser sintering technology to immobilize simulated wastes after trialkyl phosphine oxide (TRPO) process using natural granite as the host matrix. This method decreased the sintering cycle from about 10 h using traditional Joule heating to less than 5 min. The synthesized waste forms are characterized via XRD, FT-IR, XPS, and SEM-EDS to elucidate the structural change of complex aluminosilicate. Their mechanical properties and chemical durability have also been evaluated. Results show that varying from the conventional method, the laser sintered samples exhibit a perfectly regular crystal growth. As the waste loading increases (within 10–50 wt.%), more apatite crystals form in the glass-based waste forms of samples prepared by laser sintering, which facilitates the understanding of the changes in waste content. Moreover, the increased incorporated waste cations gradually depolymerize the glass network structure, promoting the transition from the glass to the glass-ceramic phase. After 28 days of leaching treatment, the normalized leaching rates of La, Ce, and Nd in the waste forms reached ∼10 −9 g·m −2 ·d −1 . These findings indicate that natural granite as well as laser sintering technology holds significant research value for the efficient immobilization of complex radioactive wastes.
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DOI
高影响因子
APPLIED SURFACE SCIENCE
·
2026
·
IF 6.6
Xin Jiang,Xiangting Yang,Huiqin Fei,Linfeng Zhang,Huadong Wu,Yoann de Rancourt de Mimérand,Xiaoyun Jin,Jia Guo
Cold plasma discharge, surface synthesis and 3D printing were used to fabricate supported photocatalysts. • Three different approaches were employed to immobilize QDs on a fractal polymer substrate. • One of the fabrication routes allowed obtaining a 1 O 2 -generating catalyst. • This hybrid catalyst was more efficient for the purification of water than the free QDs and easy to reuse. Owing to their quantum confinement, quantum dots (QDs) can efficiently generate singlet oxygen, a highly reactive oxygen species (ROS). Although promising for photocatalytic water remediation, their practical application remains limited. Their nanoscale dimensions render them more challenging to deploy than bulk heterogeneous photocatalysts; specifically, their separation and recycling are complex, and catalyst leaching poses a significant issue. In this study, we evaluated several chemical immobilization methods initiated by cold plasma discharge (CPD) to fabricate supported QD-based catalysts for the photodegradation of organic pollutants. Using CdS QDs as a model system, we probed their capacity to generate singlet oxygen. The resin substrates were fabricated by LCD 3D printing and microtextured with fractal architectures. Among the different immobilization strategies, the most successful employed a secondary nanostructured ZnO support to enforce a monolayer dispersion of the QDs. Subsequent in situ encapsulation by a metal–organic framework (MOF) further improved the photostability of the catalyst. This study provides valuable insights and new directions for the facile surface immobilization of functional QDs within controlled architectures for the efficient remediation of organic-contaminated water. Download: Download high-res image (89KB) Download: Download full-size image
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DOI
高影响因子
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