INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2019
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IF 4.784
Haiming Chen,Hui Niu,Hailing Zhang,Yonghuan Yun,Wenxue Chen,Qiuping Zhong,Weijun Chen,Xiong Fu
A ferulic acid-sugar beet pulp pectin complex (FA-SBPP) was prepared using an immobilized enzyme (Novozym 435®) as the catalyst at 60 °C in a vacuum-controlled system. The structure of FA-SBPP was characterized by FT-IR and NMR ( 1 H and 13 C). In addition, the antioxidant activity of FA-SBPP was evaluated according to the DPPH free radical scavenging ability and ORAC values. Moreover, the physical and oxidation stability of the emulsion was evaluated by particle size distribution, cream index (CI), peroxide value (POV), and 2-thiobarbituric acid reactive substance (TBARS) formation. The results showed that esterification between FA and SBPP was confirmed, and the reaction mainly occurred at the C-2, C-3, and C-6 positions. When the concentration was 1.0 mg/mL, the DPPH scavenging activity and total antioxidant activity of FA-SBPP-3 were 80.03% and 355.72 μmol TE/g, respectively. Compared with SBPP, FA-SBPP-stabilized emulsions exhibited significant smaller droplet sizes and lower CIs, POVs and TBARS amounts. Thus, the introduction of FA changed not only the chemical reactivity but also the polarity of SBPP, thereby improving its antioxidant ability and affinity for the oil-water interface. Thus, we provide a multifunctional stabilizer that can reduce the dose of antioxidants or even replace them in oil-in-water emulsions.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
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IF 8.5
Liting Zhang,Mingjun Qiao,Haoyu Zheng,Tony Vancov,Vasileios Antoniadis,Sabry M. Shaheen,Stephen Joseph,Chao Chen,Shengdao Shan,Hanbo Chen,Hailong Wang
Herein, a series of novel carboxymethyl cellulose (CMC)/pectin-based hydrogel beads were synthesized with chitosan as a cross-linker. The hydrogel composites were denoted as CPC-1/2/3/4/5, with varying mass ratios of CMC, pectin, and chitosan (6:0:1, 5:1:1, 4:2:1, 3:3:1, and 2:4:1). This is a pioneering study that investigates the synergistic effects of these biopolymers in a single hydrogel system for the adsorption of heavy metals, specifically Cd 2+ , Pb 2+ , and Cu 2+ . CPC-4 was identified as the optimal adsorbent, and its adsorption behavior, efficiency, and mechanisms were systematically explored. The adsorption kinetics of CPC-4 for Cd 2+ , Pb 2+ , and Cu 2+ adhered to the pseudo-second-order model, while equilibrium adsorption isotherms were best described by the Langmuir model. Notably, CPC-4 achieved maximum adsorption capacities for Cd 2+ , Pb 2+ , and Cu 2+ of 139.4, 270.3, and 143.6 mg/g, respectively. Spectroscopic analyses including FTIR and XPS revealed abundant oxygen- and nitrogen-bearing functional groups (carboxyl, hydroxyl, and amine) in CPC-4, which facilitated metal ion binding through complexation reactions. Density functional theory calculations demonstrated that the incorporation of chitosan significantly enhanced the interaction between CMC and pectin, identifying the coupled regions as the most favorable sites for metal adsorption, with adsorption energies of −4.03 eV for Cd, −4.59 eV for Pb, and −5.59 eV for Cu. The superior adsorption performance of CPC-4 is primarily attributed to complexation and hydrogen bonding at the cross-linked sites. In summary, this study highlights the potential of this biopolymer-based hydrogel composite as a promising new adsorbent for the effective management of heavy metal contamination in aquatic environments.
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FOOD CHEMISTRY
·
2025
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IF 9.8
Dong-Jun Hou,Quan-yuan Xie,Ming-Shun Chen,Yu-Lin Cheng,Bing Zhang,Zong-cai Tu,Lu Zhang
Ellagitannins (ETs) exhibit beneficial effects on gut microbiota modulation and bioactivity, but their instability compromises the delivery efficiency and absorption. Therefore, a double emulsion gel (DEG@RETs) loading with Rubus chingii Hu ETs (RETs) was constructed and optimized to enhance its gastrointestinal digestion stability and intestinal transformation. The optimal DEG@RETs formulation was 4 % pectin, calcium-induced gelation, W 1 /O: W 2 of 4: 6, and W 1 : O of 5: 5. Which achieved an encapsulation efficiency (EE) of 88.68 ± 4.36 %, maintained spherical structures with an EE (78.57 ± 1.34 %) after digestion. The DEG@RETs demonstrated pH and freeze-thaw stability while EE declined after heating at 90 °C. Cryo-SEM confirmed a crosslinked gel network. In vitro fermentation, transformation efficiency demonstrated significantly enhanced with urolithin A, urolithin B and ellagic acid increased by 7.37-, 3.19- and 7.87-fold respectively. These results proved that DEG@RETs can be an effective strategy to improve the colon-targeted delivery and biotransformation efficiency of RETs.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Yun-shan Wei,Kun Feng,Hong Wu
In order to modify colonic release behavior of lactoferrin (Lf), a hydrophobic composite nanofibrous carrier (CNC) was constructed by emulsion coaxial electrospinning. Ethylcellulose/pectin based water-in-oil emulsion and Lf-contained polyvinyl alcohol solution were used as shell and core fluids, respectively. An electrospinning diagram was first constructed to screen out suitable viscosity (51–82 cP) and conductivity (960–1300 μS/cm) of the dispersed phase of pectin solution for successful electrospinning of shell emulsion. Varying mass fraction of pectin solution (5 %–20 %) of shell emulsion during emulsion coaxial electrospinning obtained CNCs with different micro-structures, labeled as 5&95 CNC, 10&90 CNC, 15&85 CNC, 20&80 CNC. These CNCs all achieved colonic delivery of Lf (>95 %), and the time for complete release of Lf in simulated colon fermentation process were 10, 7, 5 and 3 h, respectively. That is, the greater the pectin content in CNC, the faster the release rate of stabilized Lf in colon. Lf release in simulated colon fermentation fluid involved complex mechanisms, in which diffusion release of Lf was dominant. Increasing colonic release rate of Lf enhanced its regulation effect on the expression levels of cell cycle arrest and apoptosis-related protein and promote its effective inhibition on the proliferation of HCT116 cell.
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CARBOHYDRATE POLYMERS
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2025
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IF 12.5
Jinyan Gu,Mouming Zhao,Lijun You,Lianzhu Lin
The colon immune activity of high branched rhamnogalacturonan-I type pectin from Lycium barbarum fruit (LBP-P4) was unclear. This study comparatively investigated the colon immunity activity of LBP-P4 and inulin and explored its mechanism via simulated colon biological and mechanical barriers models. LBP-P4 could be slowly degraded and utilized by intestinal microorganisms with degradation rate of 88.88 %. Meanwhile, LBP-P4 increased the relative abundance of Bifidobacterium, especially Bifidobacterium longum subsp. longum , which was confirmed by single Bifidobacterium fermentation. In the normal colon mechanical barrier coupled with macrophage model, the colonic metabolites of LBP-P4 enhanced macrophage viability and phagocytic ability. In the damaged colon mechanical barrier coupled with macrophage model, the colonic metabolites of LBP-P4 enhanced mechanical barrier integrity, reduced the production of NO and IL-6 and improved macrophage viability and phagocytic ability. The colon immunity activity of LBP-P4 was stronger than that of inulin, which was because the contents of immunity related metabolites i.e. short chain fatty acids and tryptophan metabolites, were significantly higher (about 1–3 fold) in LBP-P4 group than those in inulin group. Our findings provided new insights into the probiotic and intestinal immunity function of LBP-P4 and offered theoretical basis for the development of functional products of LBP-P4.
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Food & Function
·
2022
·Vol. 13
·期 8
·4384-4398
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IF 6.317
Chang Tan,Mingyue Wang,Yanwen Kong,Meizhi Wan,Haotian Deng,Yuqi Tong,Chunmao Lyu,Xianjun Meng
This study investigated the anti-inflammatory effects of cyanidin-3-glucoside (C3G) and blueberry pectin (BP) complexes on mice with dextran sodium sulfate (DSS)-induced colitis before and after high hydrostatic pressure (HHP) treatment. Real-time polymerase chain reaction (RT-PCR), western blotting, and 16S rDNA sequencing were used to study the expression of inflammation-related factors, activation of signal pathway-related proteins, and changes in the intestinal flora in ulcerative colitis (UC) mice. The results showed that HHP-treated C3G-BP complexes significantly relieved diarrhea and blood loss in the stool of UC mice and alleviated colon shortening. The potential mechanism of action involved reduction in intestinal oxidative stress mRNA expression of pro-inflammatory factors, improvement in anti-inflammatory factor levels, inhibition of the NF-κB signaling pathway, increased protein levels of Bcl-2/Bax and caspase-3/cleaved caspase-3 genes, and improved gut microbiota composition. Compared with other experimental groups, the HHP-treated C3G-BP complexes group exhibited the best anti-inflammatory effect on DSS-induced UC mice. The results may provide new ideas for using C3G-BP complexes for treating UC and help develop better processing methods.
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FOOD CHEMISTRY
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2025
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IF 9.8
Hualei Chen,Shujuan Yu,Fei Pan,Xiangxin Li,Xiaoming Fang,Wenjun Peng,Wenli Tian
The morphological features and interactions among the assembly units of nanoparticles (NPs) significantly influence their ability to stabilize Pickering emulsions. In this study, the quinone group of oxidized tannic acid (OTA) was covalently cross-linked with the amino groups in aminated sugar beet pectin (SBP-NH 2 ) to form covalent composite nanonetworks (SBP-NH 2 /OTA NNs). These NNs were subsequently conjugated with cinnamaldehyde (CA) to enhance interfacial activity, enabling the formation of 3D printable high internal phase emulsions (HIPEs). The SBP-NH 2 /OTA NNs exhibited good surface wettability (86.83 ± 1.31°) under optimal conditions and superior performance in emulsification and inhibition of droplet coalescence. Furthermore, the introduction of CA into the oil phase reinforced interfacial layers through interfacial conjugation regulation, endowing the CA-mediated HIPEs with enhanced viscoelasticity and improved 3D printing performance. Notably, CA-mediated HIPEs exhibited excellent oxidative stability and antimicrobial efficacy, offering significant potential for the development of functional food formulations.
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CARBOHYDRATE POLYMERS
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2025
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IF 12.5
Jinyan Gu,Mouming Zhao,Lijun You,Lianzhu Lin
Rhamnogalacturonan-I type pectin polysaccharide from wolfberry has immunity activity, but its intestinal immunity activity and structure-activity relationship in the small intestine was still unclear. This study comparatively investigated the intestinal immune activity of wolfberry-derived high and low branched Rhamnogalacturonan-I type pectin polysaccharides (H-LBP and L-LBP) and explored the interaction mechanism with mechanical barrier. In the normal mechanical barrier model, both H-LBP and L-LBP could cross mechanical barrier with transport rates of 23.2 % and 25 %, thereby directly enhancing macrophage viability and phagocytic ability after crossing the mechanical barrier. The transport mechanism of H-LBP in mechanical barrier included the clathrin- and caveolin-mediated pathways. In the damaged mechanical barrier model, H-LBP could significantly enhance mechanical barrier integrity, reduce the production of neurotransmitter (NO) and inflammatory cytokine (TNF-α), thereby exerting indirectly intestinal immune activity. Transcriptome analysis showed that the interaction mechanism between H-LBP and damaged mechanical barrier mainly involved signaling pathway regulating cell growth and survival (PI3K-AKT). Western blot experiment and molecular docking simulation confirmed that H-LBP could reduce the expression of p-PI3K, p-AKT and cleaved Caspase3. H-LBP had stronger directly and indirectly intestinal immune activity than L-LBP. These findings were useful for the application of H-LBP in improving intestinal immunity oral formulations.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Meiyu Zheng,Wenjuan Zhang,Shengmin Lu
Naringin (NR) and hydroxypropyl-β-cyclodextrin (HPCD) can form a water-soluble complex, but it is unstable. This study aimed to investigate the characterization of the pectin/alginate hydrogel nanoparticles (HNPs) loading HPCD-complexed naringin. The encapsulation efficiency and loading capacity of the HNPs for NR were found to be 79.23 % ± 1.31 % and 23.79 % ± 0.67 %, respectively. HNPs had an average diameter of 409.5 ± 8.5 nm, a PDI of 0.237 ± 0.014, and a zeta-potential of −33.5 ± 0.2. FTIR, XRD, and DSC analysis confirmed that the NR-HPCD complex was embedded into the HNPs. In simulated gastrointestinal digestion, the HNPs exhibited a lower cumulative release rate compared to free NR. In Caco-2 cells, the HNPs were more efficiently transported into the cells. Consequently, the HNPs resulted in a greater decrease in ROS levels, more recovery of mitochondrial membrane potential and higher content of glutathione. This study provided a carrier for encapsulating NR, making it possible for use in food or functional food.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2026
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IF 5.4
Peiyao Wu,Yujia Liu,Weijie Li,Wenjun Guan,Jiayi Zeng,Peiyi Huang,Elin Li,Chunfeng Zhang,Yunyou Lv,Fang Yang,Jie Liu
Bioinspired Pickering emulsions were fabricated via controllable oil-water interfacial self-assembly. • Pectin methyl esterification tailored particle adsorption and interfacial assembly behavior. • A particle-stabilized interface enabled synergistic pH/pectinase-responsive on-demand release of abamectin (AVM). • Interfacial engineering improved droplet stability, spreading, adhesion, and rainfastness. • Optimized emulsions achieved efficient pest control with enhanced biosafety and environmental compatibility. The Asian corn borer ( Ostrinia furnacalis Guenée ) is a major pest threatening maize production; however, the practical application of abamectin (AVM) is limited by poor environmental stability, insufficient foliar retention, and uncontrolled release behavior. Herein, a bioinspired Pickering emulsion delivery system (ACH X ) was constructed using modified pectin and carboxymethyl chitosan (CMCS) for efficient AVM delivery. Inspired by natural biological interfaces, ACH X was designed to reproduce functional characteristics including protective capability, foliar adhesion, and stimulus-responsive activation rather than specific biological structures. By regulating pectin methyl esterification, the interfacial assembly behavior was precisely controlled, enabling enhanced leaf deposition and pH/pectinase dual-responsive release. The particle-stabilized interface improved AVM environmental stability, with thermal degradation rates maintained below 20% and approximately 60% active ingredient retention after ultraviolet irradiation. Meanwhile, ACH X exhibited enhanced droplet spreading and retention on hydrophobic leaf surfaces, with stable spreading diameters of 1.78-1.83 mm and a rebound height reduction of approximately 0.15 mm compared with free AVM. Under simulated alkaline and pectinase-rich pest midgut conditions, ACH X achieved over 95% cumulative AVM release while maintaining limited release under non-target conditions. Benefiting from adaptive deposition and target-responsive release, ACH X achieved a 24 h mortality of 96.66% against Ostrinia furnacalis Guenée , together with improved field control performance and favorable environmental compatibility. This study provides a functional biomimetic interfacial engineering strategy for developing intelligent and sustainable nano-pesticide delivery systems. Download: Download high-res image (168KB) Download: Download full-size image
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FOOD RESEARCH INTERNATIONAL
·
2022
·
IF 7.425
Qiwei Du,Yang Wu,Xiaoqun Zeng,Maolin Tu,Zhen Wu,Jianhua Liu,Daodong Pan,Yuting Ding
Whey protein hydrolysate (WPH), maltodextrin (MD), low methoxy pectin (LMP) and high methoxy pectin (HMP) were used to study the interface binding under high temperature sterilization conditions (121 °C, 15 min). The effect of competitive binding of MD and pectin with interface protein on the storage stability and gastrointestinal fate of fish oil emulsion was studied. The low-molecular-weight MD and the interface protein undergo a wide range of covalent binding through the Maillard reaction, while a small amount of high-molecular-weight pectin can form a protective shell with the interface protein through electrostatic interaction to inhibit the covalent reaction of MD, which was called competitive binding. However, due to the bridging and depletion flocculation of pectin, the emulsification stability of fish oil emulsion reduced. After 13 days of storage, compared with the particle size of the WPH fish oil emulsion (459.18 nm), the fish oil emulsion added with LMP and HMP reached 693.58 nm and 838.54 nm, respectively. In vitro digestion proved that WPH fish oil emulsion flocculated rapidly in the stomach (1.76 μm), while WPH-MD and WPH-MD-pectin fish oil emulsions flocculated slightly (less than800 nm). WPH-MD-pectin delayed digestion in the gastrointestinal tract, and HMP exhibited a better slow-release effect. This study provides reference for the design of multi-component functional drinks and other bioactive ingredient delivery system.
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FOOD HYDROCOLLOIDS
·
2026
·
IF 13.8
Mingrui Chen,Yuan Zhou,Xi Wei,Xiaojing Shen,Weijie Lan,Qing Zhang,Yaowen Liu
To efficiently recover rhamnogalacturonan I (RG-I) entrapped within the recalcitrant apple cell wall matrix, the present study established a two-step strategy combining polyol-based deep eutectic solvent (DES)/cellulase (CE) pretreatment and extraction using water. The highest CE activity was achieved in choline chloride (ChCl)/propylene glycol (PG) at molar ratio of 1:2 with water content of 23.57 wt% among twenty polyol-based DESs tested. The Kamlet-Taft parameters (α and β) of hydrated ChCl/PG showed positive correlations with CE activity (r = 0.85 for α, 0.88 for β), whereas viscosity and polarity (π∗) exhibited negative correlations (r = −0.56 for viscosity, −0.73 for π∗). Extraction using the ChCl/PG/CE-assisted water method yielded the highest recovery (17.8%), significantly exceeding those achieved with CE alone, ChCl/PG/CE alone or ChCl/PG-assisted water extraction. Compared with extracted control pectin (E-P, ChCl/PG-P), ChCl/PG&E-P contained the highest RG-I content with more/longer neutral sugar side chains (RG-I = 81.28 mol%, (Ara + Gal)/Rha = 4.50). Efficient hydrolysis of amorphous cellulose, coupled with enhanced interactions between ChCl/PG and exposed RG-I, synergistically promoted the dissociation of RG-I from cellulose-RG-I matrix and its subsequent dissolution in water. Finally, ChCl/PG&E-P was capable of forming stable gel in low concentration and a small amount of Ca 2+ . Due to the higher content of side chains, ChCl/PG&E-P gel exhibited higher gel strength and rheological moduli (G′ and G″) across varying pectin/Ca 2+ concentrations and pH conditions. This work offers a novel strategy for the efficient recovery of RG-I-enriched pectin from apple cell wall, which exhibits good characteristics as a gelling agent.
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