MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 11
·1-7
·
IF 5.705
Jia Haiyan,Tian Qingyun,Xu Jingkun,Lu Limin,Ma Xue,Yu Yongfang
A three-dimensional porous network graphene aerogel (GAs) with large specific area and excellent conductivity was loaded with β-cyclodextrin polymer (Pβ-CD) to serve as a support for immobilization of antibodies. A highly sensitive immunosensor for the cancer marker carbohydrate antigen 15–3 (CA15–3) was designed based on the use of Pβ-CD/GAs. The large specific area of GAs warrants high loading with antibodies, and their excellent electrical conductivity warrants strong electrical signals. Based on the synergistic effect of GAs and Pβ-CD, an immunoassay was designed that is making use of hexacyanoferrate as an electrochemical probe and having a pleasantly low working potential of 0.2 V (vs. SCE). Response is linear in the 0.1 mU mL −1 to 100 U mL −1 activity range, and the lower detection limit is 0.03 mU mL −1 (at S/ N = 3). The immunoassay is stable, selective and reproducible. It was applied to the analysis of spiked samples, and results were satisfactory.
Schematic of an electrochemical immunoassay for the carbohydrate antigen 15–3. It is based on the use of β-cyclodextrin polymer and a graphene aerogel.
研究领域:
生物医学工程
·
分析化学
✓ 已验证
DOI
PubMed
高影响因子
Food Hydrocolloids for Health
·
2025
·
IF 5.1
Adila Abdirym,Wu Xue,Liu Bin
This work investigated the stabilization mechanisms and β-carotene encapsulation characteristics of cellulose nanofibrils (CNFs) stabilized Pickering emulsions, comparing different emulsification processes. For the 48 days storage, Pickering emulsions (PE) made by ultrasounds (US) and employed at least 2.0 wt.% CNFs as emulsifiers/stabilizers has obvious cream stabilization, and PE made by high pressure homogenization (HPH) and employed at over 1.2 wt.% CNFs can have rather excellent stabilization. The stabilization of HPH emulsions which was superior to that of US emulsions mainly relied on steric stabilization of CNFs space networks. Although the β-Carotene encapsulation efficiency of US emulsions was higher than that of HPH emulsions when the CNFs concentration was over 1.2 wt.%, the β-Carotene retention rate of US emulsions was obviously lower than that of HPH emulsions. So, the internal space structure of CNFs stabilized Pickering emulsions made by HPH was conducive to stabilizing emulsion and protecting bioactive molecule.
研究领域:
食品科学与技术
·
纳米材料
·
胶体与界面科学
·
封装技术
✓ 已验证
DOI
高影响因子
Frontiers in Pharmacology
·
2021
·
IF 5.811
Yang Siwen,Qu Yunhe,Chen Jiyu,Chen Si,Sun Lin,Zhou Yifa,Fan Yuying
Insufficient pancreatic β-cell or insulin-producing β-cell are implicated in all types of diabetes mellitus. Our previous studies showed bee pollen polysaccharide RBPP-P improves insulin resistance in type 2 diabetic mice by inhibiting liver fat deposition. However, its potential of regulating β-cell function and integrity is not fully known. Herein, we observed that β-cell proliferation (n = 10), insulin synthesis (n = 5, p = 0.01684) and insulin incretion (n = 5, p = 0.02115) were intensely activated in MIN6 cells when treatment with RBPP-P. In alloxan-induced diabetic mice, oral administration of RBPP-P (n = 10) effectively decreased the blood glucose (p = 0.0326), drink intake (p < 0.001) and urine (p < 0.001). It directly stimulated phosphorylation of p38 (p = 0.00439), ERK (p = 0.02951) and AKT (p = 0.0072) to maintain the islet function and mass. Thus, our data suggest that RBPP-P is a natural compound to regulate β-cell proliferation and function, indicating it might have therapeutic potential against type 1 diabetes.
研究领域:
药理学
·
内分泌学
·
糖尿病研究
✓ 已验证
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2021
·
IF 7.963
Muhammad Usman,Adeel Ahmed,Zhijian Ji,Bing Yu,Youqing Shen,Hailin Cong
Heavy metals and endocrine disrupters often co-exist in wastewater, while their possible competition behaviours make uptake removal more challenging. Therefore, β-Cyclodextrin based nanocomposite adsorbent was successfully fabricated (β-Cyclodextrin/ZrO 2 ) for the simultaneous uptake of Pb(II) and Bisphenol A from wastewater. FTIR, XRD , and XPS confirmed the successful fabrication of the β-Cyclodextrin/ZrO 2 nanocomposite. In this setting, oxygen-containing groups are primarily responsible for the Pb(II) binding, while the β-Cyclodextrin cavities adsorb Bisphenol A through host-guest interaction, enabling the simultaneous removal of Pb(II) and Bisphenol A. In the mono contaminant system, the nanocomposite displayed prominent removal ability toward Pb(II) and Bisphenol A with adsorption characteristics of pseudo-second-order, Langmuir, and Freundlich isotherm model. The maximum adsorption capacities were identified for Pb(II) and Bisphenol A to be 274.4 mg/g and 174.9 mg/g at 298 K, respectively. Most importantly, the β-Cyclodextrin/ZrO 2 could efficiently attain simultaneous removal of Pb(II) and Bisphenol A by avoiding their competitive behaviours was due to the different adsorption mechanisms (electrostatic interaction and host-guest interaction). Moreover, the adsorbed Pb(II) and Bisphenol A could be successfully recovered with a slight decline in nanocomposite removal performance even after 4 cycles in the binary-component system. All these findings provide insights into the fabrication of highly effective adsorbent with separated adsorption sites to treat wastewater bearing heavy metal and endocrine disrupters.
研究领域:
环境科学
·
纳米技术
·
水处理
·
材料科学
·
吸附技术
✓ 已验证
DOI
高影响因子
JOURNAL OF CONTROLLED RELEASE
·
2024
·
IF 10.5
Tao Bo,Chenfei Wang,Dingjin Yao,Qiuyu Jiang,Yitong Zhao,Feifei Wang,Wei He,Weiyi Xu,Hao Zhou,Ming Li,Si Zhang,Ruyi Xue
Gene delivery to macrophages holds great promise for cancer immunotherapy. However, traditional gene delivery methods exhibit low transfection efficiency in macrophages. The star -shaped topological structure of polymers is known to encapsulate genes inside their cores, thereby facilitating sustained release of the genetic material. Herein, combining the structural advantages of star polymers and the transfection advantages of poly (β-amino ester)s (PAEs), we developed a novel linear oligomer grafting-onto strategy to synthesize a library of multi-terminal star structured PAEs (SPAEs), and evaluated their gene delivery efficiency in various tissue cells. The transfection with human hepatocellular carcinoma cells (HepG2, HCC-LM3 cells and MHCC-97H cells), rat normal liver cells (BRL-3 A cells), human ovarian cancer cells (A2780 cells), African green monkey kidney cells (Vero cells), human cervical cancer cells (HeLa cells), human chondrosarcoma cells (SW1353 cells), and difficult-to-transfect human epidermal keratinocytes (HaCaT cells) and normal human fibroblast cells (NHF cells) showed that SPAEs exhibited superior transfection profile. The GFP transfection efficiency of top-performing SPAEs in HeLa cells (96.1%) was 2.1-fold, and 3.2-fold higher compared to jetPEI and Lipo3000, respectively, indicating that the star-shaped topological structure can significantly enhance the transfection efficiency of PAEs. More importantly, the top-performing SPAEs could efficiently deliver Nod2 DNA to difficult-to-transfect RAW264.7 macrophages, with a high transfection efficiency of 33.9%, which could promote macrophage M1 polarization and enhanced CD8+ T cell response in co-incubation experiments. This work advances gene therapy by targeting difficult-to-transfect macrophages and remodeling the tumor immune microenvironment.
研究领域:
生物技术
·
药学
·
免疫学
·
癌症研究
✓ 已验证
DOI
高影响因子
TEXTILE RESEARCH JOURNAL
·
2017
·
IF 1.443
Ruijun Wang,Zhengchu Liu,Lifeng Cheng,Shengwen Duan,Xiangyuan Feng,Ke Zheng,Yi Cheng,Jie Zeng
Xylanase plays an important role in the hydrolysis of hemicellulose and has gained much attention in the field of biological degumming. The research for xylanases with cellulase-free and high activity for biological degumming has intensified in recent years. In the present research, heterologous expression of a novel endo-β-1,4-xylanase (GH30) from Dickeya dadantii DCE-01 in Escherichia coli BL21 (DE3) was reported. Biochemical characterization of the enzyme and a potential application in ramie biological degumming was discussed. The results showed that the xylanase gene consists of 1251 nucleotides, belonging to glycoside hydrolase family 30 (GH30). The optimal activity of the xylanase was observed at 50℃ and a pH value of 6.4. The Km and Vmax values for beechwood xylan were 14.25 mg/mL and 296.6 μmol/mg, respectively. The catalytic activity was enhanced by addition of 1 mM Cu2+, Ca2+, Mg2+, and K+. The recombinant enzyme was specific for xylan substrates. The enzyme exhibited hydrolytic activity toward ramie hemicellulose. The recombinant xylanase could be effectively applied to ramie degumming.
研究领域:
生物化学
·
生物技术
·
酶学
·
纺织科学
✓ 已验证
DOI
高影响因子
Journal of Materials Chemistry B
·
2016
·Vol. 4
·期 22
·3903-3910
·
IF 4.872
Genxing Zhu,Ruibo Zhao,Yaling Li,Ruikang Tang
Multifunctional Gd,Ce,Tb co-doped β-tricalcium phosphate (TCP) porous nanospheres are prepared by a facile solvothermal strategy with trimethyl phosphate as the phosphorus source. The as-prepared nanomaterial (average diameter of 100 nm) has a multiple level pore size distribution with the specific surface area of 124.33 m2 g−1, which benefits drug loading. Its photoluminescent and magnetic multifunctions are realized by the co-doping of Gd3+, Ce3+ and Tb3+ ions, which make the nanomaterial promising for both fluorescence and magnetic resonance imaging techniques. Furthermore, the nanomaterial exhibits excellent cytocompatibility and a relatively high doxorubicin loading capacity as well as sustained pH-sensitive drug release behaviour. It is suggested that the Gd,Ce,Tb co-doped β-TCP porous nanospheres are promising for applications in the biomedical fields such as multifunctional drug delivery systems and tissue engineering scaffolds with bioimaging guidance.
研究领域:
材料科学
·
纳米技术
·
生物医学工程
·
药剂学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF POLYMER ENGINEERING
·
2020
·Vol. 40
·期 5
·440-447
·
IF 1.126
Kuanmin Chen,Suoju He,Hui Wang,Song Zhang,Lizhen Yu,Yue Zhang,Ezzat H Elshazly,Lixia Ke,Renmin Gong
In this paper, the β-cyclodextrin (β-CD) and biotin (Bi) were successfully grafted onto carboxymethyl chitosan (CMCS). And then the β-CD-Bi-CMCS nanoparticles (NPs) were prepared as oral nano-delivery carrier of protein drugs by ionic gelation method. The morphological feature of fabricated drug carrier was determined by dynamic light scattering and transmission electron microscopy. The result showed that the prepared NPs presented spherical structure with an average diameter of 138 nm. Bovine serum albumin (BSA) was selected as model protein drug that was entrapped in prepared drug carrier with satisfactory entrapment efficiency (79.18%) and loading content (3.96%). The drug release profiles of BSA/β-CD-Bi-CMCS NPs were studied at different pH environment for simulated gastric fluid (SGF), simulated intestinal fluid (SIF) and simulated colonic fluid (SCF). It was found that the BSA/β-CD-Bi-CMCS NPs displayed a pH dependent drug release profiles. After 72 h, the cumulative release amount of BSA in SGF, SIF, and SCF was about 20.57, 74.46, and 91%, respectively. Furthermore, the enzymatic degradation and cytotoxicity studies showed the synthesized β-CD-Bi-CMCS NPs had high chemical stability and biocompatibility. This work indicated that the β-CD-Bi-CMCS NPs had the potentiality as promising nanocarriers for oral delivery of protein drugs.
研究领域:
药剂学
·
纳米技术
·
药物递送系统
·
生物医学工程
✓ 已验证
DOI
高影响因子
Biomed Research International
·
2022
·Vol. 2022
·期 1
·5648896
·
IF 3.246
Xue-Qin Li,Shan-Shan Jia,Ke Yuan,Song-Heng Jin
Content . The water and ethanol extracts of Torreya grandis ( T. grandis ) have antioxidant, antibacterial, and hypolipidemic effects . Objective . The hypoglycemic effect and mechanism of the n-butanol fraction of the 75% ethanol extract of T. grandis leaves (BFTL) on rats with streptozotocin- and high-fat diet-induced type 2 diabetes mellitus (T2DM) were investigated. Materials and Methods . Sprague–Dawley rats were randomly divided into six groups: control, T2DM, metformin, high-dose BFTL (800 mg/kg), middle-dose BFTL (400 mg/kg), and low-dose BFTL (200 mg/kg). After 4 weeks of BFTL treatment, the correlations of serum indicators with protein expression in tissue were determined, and pathological changes in the liver, kidneys, and pancreas were analyzed. Results . Compared with the results in the T2DM group, serum fasting blood glucose, triglyceride, total cholesterol, malondialdehyde, alanine aminotransferase, and aspartate aminotransferase levels were significantly decreased ( p < 0.05), whereas superoxide dismutase and glutathione peroxidase levels were significantly increased ( p < 0.05) in the high-, middle-, and low-dose BFTL groups. The treatment also improved oral glucose tolerance. In addition, the pathological changes of the liver, kidney, and pancreas were improved by BFTL treatment. Cytochrome and caspase-3 expression in pancreatic was significantly decreased ( p < 0.05) by BFTL treatment, whereas the Bcl-2/Bax ratio was significantly increased ( p < 0.05). Discussion and Conclusion . BFTL exerted significant hypoglycemic effect on T2DM model rats, and its mechanism involved the suppression of blood glucose levels and oxidative stress by improving the metabolism of blood lipids and antioxidant capacity, boosting β -cell function, and inhibiting β -cell apoptosis.
研究领域:
药理学
·
糖尿病学
·
分子生物学
✓ 已验证
DOI
高影响因子
Food Biophysics
·
2024
·Vol. 19
·期 2
·310-320
·
IF 2.8
Wu Zi-ke,Chen Ru-kang,Shi Yu,Zhu Chen-min,Wang Yi-ran,Shi Yu-gang,Ettelaie Rammile,Gu Qing
Octyl gallate (GAC8) as a bioactive compound has excellent antibacterial effectiveness, but its poor hydrophilicity limits its applications. In this work, GAC8 was encapsulated into hydroxypropyl- β -cyclodextrin (HP β CyD) cavity to form an inclusion complex (GAC8/HP β CyD-IC), and their antibacterial activities were investigated. Phase solubility test suggested that the aqueous solubility of GAC8 was prominently enhanced after forming the inclusion complex. The aqueous solution of GAC8/HP β CyD-IC yielded uniform fiber morphology with ~ 900 nm average fiber diameter. The fabricated GAC8/HP β CyD-IC nanofibers (GAC8/HP β CyD-IC NFs) were characterized by 1 H NMR, FT-IR, XRD, DSC, and TGA, revealing successful synthesis of GAC8/HP β CyD-IC NFs and the thermal stability of GAC8 was enhanced by inclusion complexation with HP β CyD. Furthermore, GAC8/HP β CyD-IC NFs possessed antibacterial activity against E. coli (12.5 mm zone of inhibition), S. aureus (18.5 mm zone of inhibition). The results of DNA and protein leakage in the experiment indicated that GAC8/HP β CyD-IC NFs can disrupt the membrane integrity of bacteria. Meanwhile, GAC8/HP β CyD-IC NFs suppressed the colony growth of E. coli on Chinese giant salamander meat. Overall, the nanofibers encapsulating GAC8/HP β CyD-IC were potential antibacterial food packaging materials.
研究领域:
食品包装
·
抗菌材料
·
纳米技术
·
食品保鲜
·
高分子科学
✓ 已验证
DOI
高影响因子
JOURNAL OF CELLULAR PHYSIOLOGY
·
2024
·
IF 4.5
Zhiguang Chen,Qi Zhao,Lianghong Chen,Songlan Gao,Lingshuai Meng,Yingjie Liu,Yu Wang,Tiegang Li,Jinqi Xue
Osteogenic differentiation is important for fracture healing. Microfibrial-associated glycoprotein 2 (MAGP2) is found to function as a proangiogenic regulator in bone formation; however, its role in osteogenic differentiation during bone repair is not clear. Here, a mouse model of critical-sized femur fracture was constructed, and the adenovirus expressing MAGP2 was delivered into the fracture site. Mice with MAGP2 overexpression exhibited increased bone mineral density and bone volume fraction (BV/TV) at Day 14 postfracture. Within 7 days postfracture, overexpression of MAGP2 increased collagen I and II expression at the fracture callus, with increasing chondrogenesis. MAGP2 inhibited collagen II level but elevated collagen I by 14 days following fracture, accompanied by increased endochondral bone formation. In mouse osteoblast precursor MC3T3-E1 cells, MAGP2 treatment elevated the expression of osteoblastic factors (osterix, BGLAP and collagen I) and enhanced ALP activity and mineralization through activating β-catenin signaling after osteogenic induction. Besides, MAGP2 could interact with lipoprotein receptor-related protein 5 (LRP5) and upregulated its expression. Promotion of osteogenic differentiation and β-catenin activation mediated by MAGP2 was partially reversed by LRP5 knockdown. Interestingly, β-catenin/transcription factor 4 (TCF4) increased MAGP2 expression probably by binding to MAGP2 promoter. These findings suggest that MAGP2 may interact with β-catenin/TCF4 to enhance β-catenin/TCF4's function and activate LRP5-activated β-catenin signaling pathway, thus promoting osteogenic differentiation for fracture repair. mRNA sequencing identified the potential targets of MAGP2, providing novel insights into MAGP2 function and the directions for future research.
研究领域:
骨骼生物学
·
骨科
·
分子生物学
·
细胞生物学
✓ 已验证
DOI
PubMed
高影响因子
Materials Science & Engineering C-Materials for Biological Applications
·
2020
·
IF 5.88
Rijian Song,Xuan Mao,Shunqing Tang
Here we prepared a low-degree-sulfated κ/β-carrageenan oligosaccharide (L-DS-KOS) by DMSO-methanol desulfation method, and fabricated a sponge dressing for the wound healing of diabetic rats. The immunomodulatory effects of L-DS-KOS on M1-like macrophages were evaluated. Results showed that L-DS-KOS could effectively promote the secretion of anti-inflammatory factors and accelerate polarization of LPS-activated macrophages from M1 to M2 type. The gross examination result showed that the sponge dressing with the mass ratio of L-DS-KOS: collagen = 3: 7 could effectively accelerate the repair process of the full-thickness excisional wound in diabetic rats; and H&E and Masson staining results disclosed that the L-DS-KOS/collagen dressing could better shorten the inflammation period of the wound site, and improve the process of collagen deposition and epithelial formation, thereby promote the repair of skin wounds in diabetic rats. These results demonstrate that L-DS-KOS has potential to be used as a new type of immunomodulatory biomaterial for diabetic wound dressings.
研究领域:
免疫学
·
药剂学
·
伤口愈合
·
生物材料
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）