SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Fang Hu,Wancun Zhang,Jiaqi Zhang,Qi Zhang,Tao Sheng,Yueqing Gu
In this study, a simple, economic, and label-free electrochemical biosensor was developed for highly sensitive and selective miR-21 detection based on Fe(CN) 6 3− /Fe(CN) 6 4− electrochemical signal, which relies on DNA structures conjugating with carboxyl multi-wall carbon nanotubes (MWCNTs-COOH)-modified glassy carbon electrode (GCE). High sensitivity and specificity of the biosensor were achieved by taking advantage of the target-recycled non-enzymatic amplification strategy (TRNEAS), which relies on sequence-specific hairpin strand displacement and does not require the addition of environment-susceptible enzymes. Besides, the SP-DNA strictly binds to the cMWCNTs/GCE via amide bonds, which ensure good electrochemical results and excellent stability for target miRNA detection. The developed miR-21 electrochemical biosensor exhibited a broad linear dynamic range of 0.1 fmol to 5 pmol and a detection limit of 56.7 amol for miR-21 detection in vitro . The relative miR-21 expression levels were particularly detected by our developed miR-21 biosensor and were highly consistent with the stem-loop RT-PCR results. In summary, our developed electrochemical biosensor exhibits great potential for further application in biomedical research and early clinical diagnosis.
研究领域:
生物传感器
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电化学
·
纳米技术
·
分子生物学
·
临床诊断
✓ 已验证
DOI
高影响因子
TETRAHEDRON-ASYMMETRY
·
2014
·
IF 2.165
Rui-Jie Chen,Gao-Wei Zheng,Yan Ni,Bu-Bing Zeng,Jian-He Xu
A new NADH-dependent carbonyl reductase Rh CR capable of efficiently reducing the ε-ketoester ethyl 8-chloro-6-oxooctanoate (ECOO) to give ethyl ( S )-8-chloro-6-hydroxyoctanoate [( S )-ECHO], an important chiral precursor for the synthesis of ( R )-α-lipoic acid, was identified from Rhodococcus sp. ECU1014. Using recombinant Escherichia coli cells expressing Rh CR and glucose dehydrogenase used for the regeneration of cofactor, 440 g L −1 (2 M) of ECOO were stoichiometrically converted to ( S )-ECHO in a space–time yield of 1580 g L −1 d −1 without the external addition of any expensive cofactor.
研究领域:
生物催化
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酶工程
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合成化学
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生物技术
✓ 已验证
DOI
高影响因子
ANALYST
·
2011
·Vol. 136
·期 24
·5283-5286
·
IF 3.913
Yiqun Tan,Jiancan Yu,Yuanjing Cui,Yu Yang,Zhiyu Wang,Xiaopeng Hao,Guodong Qian
A novel fluorescence chemosensor based on 2,6-dicarbonylpyridine was designed and synthesized and its photophysical properties were characterized. Upon coordination of Co2+ by the central 2,6-dicarbonylpyridinyl functional group, the chemosensor 2,6-bis(4-diphenylamino-styrylcarbonyl)pyridine (PhPy) showed nearly complete fluorescence quenching, while no fluorescence response was seen towards other competing cations. The experimental results show the chemosensor is highly selective and sensitive towards Co2+ in the presence of competing ions, even in the ppb range. Job plot analysis was carried out and the results suggested that the binding of PhPy and Co2+ was probably a 2 : 1 stoichiometry.
研究领域:
分析化学
·
材料科学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF POLYMER RESEARCH
·
2021
·Vol. 28
·期 12
·1-9
·
IF 3.097
Li Mengqing,Zhang Yudong,Zhu Fang,Zhao Xinrui,Li Runming,Wang Hongxia,Liu Jichun,Li Xiying,Chang Haibo,Lin Tong
Poly(ε-caprolactone) ( PCL)/polyamide 6 (PA6) composites were fabricated by dispersing PA6 powder into PCL. The SEM results show good dispersion of PA6 in the PCL matrix. Crystallization behavior, crystal structure and spherulitic morphology of pure PCL and the PCL/PA6 composites, as well as their thermal stability and dynamic mechanical properties, were characterized using various techniques including differential scanning calorimeter, X-ray diffraction (XRD), polarized light microscope (PLM), thermal gravimetric analysis (TGA) and dynamic mechanical analyzer (DMA). The PCL/PA6 composite showed increased storage modulus and glass transition temperature (T g ). PA6 powder functions as a heterogeneous nucleate to promote PCL crystallization but does not alter orthorhombic crystal form, crystallization mechanism and thermal stability of PCL.
研究领域:
材料科学
·
高分子化学
✓ 已验证
DOI
高影响因子
ACS Synthetic Biology
·
2025
·
IF 3.9
Jing Wu,Ziqian Xiao,Yuhui Du,Minglan Luo,Jiacheng Zhang,Pei Guo,Zhipeng Chen,Binyuan Yang,Tingjian Chen
Aptamer-based sensors (aptasensors) have been extensively employed in biosensing and diagnostics. However, the broader application of aptasensors fabricated with DNA and RNA aptamers has been limited due to the intolerance of DNA and RNA to nucleases in biological fluids. A majority of sugar-modified nucleic acids, in particular 2’-F and 2’-OMe-modified nucleic acids, demonstrate a remarkable resistance to nuclease degradation and thus hold great potential in the development of more stable aptamers and aptasensors. In this work, employing enzymes including a laboratory-evolved polymerase for 2’-modified nucleic acids, we carried out systematic evolution of ligands by exponential enrichment (SELEX) experiments to obtain 2’-F/OMe-RNA aptamers for Bacillus cereus 5/B/6 metallo-β-lactamase (B. cereus 5/B/6 MBL), a crucial marker of many penicillin-resistant strains. Three aptamers composed of 2’-OMe-A, 2’-F-U, 2’-F-C, and 2’-OMe-G nucleotides (Apt-FUC1, Apt-FUC2, and Apt-FUC3) have been obtained and systematically characterized. All of them exhibited excellent target binding affinities, with equilibrium dissociation constant (Kd ) values of 95.9, 70.0, and 81.3 nM, and outstanding biological stability. Combining FAM-labeled Apt-FUC1, Apt-FUC2, and Apt-FUC3 with graphene oxide (GO), which could quench the fluorescence of FAM, we successfully constructed three sensitive and biologically stable 2’-F/OMe-RNA aptasensors for B. cereus 5/B/6 MBL, with the limit of detection (LOD) values of 0.38, 0.27, and 0.29 nM. Moreover, these three aptasensors all demonstrated good specificity for target detection in biological samples. This work has established a streamlined strategy for generating stable 2’-F/OMe-RNA aptamers and aptasensors, which should broaden the application of sugar-modified nucleic acids in biosensing and diagnostics.
研究领域:
分子诊断
·
核酸工程
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生物传感器
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酶检测
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抗生素耐药性
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合成生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Redox Biology
·
2025
·
IF 11.9
Xiaoyue Lai,Xiaoqin Wan,Xinyu Bao,Qingyuan Yang,Wei Zhang,Yan Tan,Xiaoshi Cai,Ziyang Wang,Yujie Li,Chun Liu,Zhongfang Deng,Pan Zheng,Dingyuan Tian,Ming Chen,Hongjun Yin,Gang Xu,Qi Huang,Zhihui Zhang,Hongmei Xia,Fang Deng
Hypoxia leads to decreased synthesis of 5-Methoxytryptophan (5-MTP) in pulmonary microvascular endothelial cells by downregulating Asmt level via Hif1α induced NF-κB p50 nuclear translocation. 5-MTP can reduce the localization of Prdx6 to lysosomes and increase its protein level by reducing its lysosomal degradation through interacting with Prdx6-S32, without affecting its function of clearing lipid peroxides (LPOs), thus improving the lipid peroxidation and rescuing VE-cadherin level and endothelial barrier function.
研究领域:
呼吸病学
·
分子生物学
·
氧化应激
·
血管生物学
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重症医学
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信号转导
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INORGANIC CHEMISTRY
·
2016
·
IF 4.82
Bing-Ping Yang,Chun-Li Hu,Xiang Xu,Jiang-Gao Mao
A new series of platinum iodates, namely, α-(H3O)2Pt(IO3)6, β-(H3O)2Pt(IO3)6, and A2Pt(IO3)6 (A = Na, K, Rb, Cs), have been synthesized. Interestingly, among these six stoichiometrically identical compounds, α-(H3O)2Pt(IO3)6 is polar, whereas other compounds are nonpolar and centrosymmetric. They all consist of zero-dimensional [Pt(IO3)6]2– molecular units separated by H3O+ or A+ cations. All of the lone electron pairs of the IO3– groups are aligned and almost point to one direction for α-(H3O)2Pt(IO3)6, whereas IO3– groups are located trans to each other in other compounds. The material, α-(H3O)2Pt(IO3)6, exhibits very strong second harmonic generation (SHG) effects, approximately 1.2 × KTiOPO4 (KTP), and is phase-matchable. Thermogravimetric analysis, elemental analysis, infrared spectra, UV–vis spectra, nonlinear optical properties, and theoretical calculations are also reported.
研究领域:
材料科学
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化学
·
光学
✓ 已验证
DOI
PubMed
高影响因子
CRYSTENGCOMM
·
2023
·Vol. 25
·期 36
·5085-5091
·
IF 3.1
Zhi-Yuan Yue,Rong-Meng Liao,Wang Luo,Na Wang,Le-Ping Miao,Heng-Yun Ye,Chao Shi
Organic–inorganic hybrid molecular ferroelectrics have attracted broad attention in the field of research of ferroelectric materials. Following the surge of stable candidates, hybrid bimetal molecular-based materials have been recently explored to achieve excellent ferroelectricity. However, rare hybrid bimetal molecular ferroelectrics were reported. Inspired by “ferroelectrochemistry”, a chiral nitrite-based hybrid bimetal molecular ferroelectric material, [(R)-3-quinuclidinol]2[LiCo(NO2)6] (1), was reported. The ferroelectric-paraelectric phase transition (C2 ↔ P321) behaviour of 1; was found at 343 K (Curie temperature, Tc) with a spontaneous electronic polarization (Ps) of 0.52 μC cm−2 and a coercive field (Ec) of 48 kV cm−1 at 298 K. Various-temperature single crystal structural analysis and dielectric constant measurements reveal that the rapid rotation of the organic cation and slight order–disorder transition of the NO2− anion are the origin of the ferroelectricity. To our knowledge, 1 is the first example of a nitrite-based hybrid bimetal molecular ferroelectric material that is triggered by an external electric field. This finding establishes a clear stimulus–structure–response relationship in advanced hybrid ferroelectric materials.
研究领域:
材料科学
·
物理化学
✓ 已验证
DOI
高影响因子
ORGANOMETALLICS
·
2016
·
IF 4.186
Wenlong Luo,Antai Li,Shaofeng Liu,Hongqi Ye,Zhibo Li
A series of chromium(III) complexes, LCrCl3 (Cr1–Cr4: L = 2-(N-R2-benzimidazol-2-yl)-6-(3,5-R1-pyrazol-1-yl)pyridine; Cr1: R1 = H, R2 = H; Cr2: R1 = Me, R2 = H; Cr3: R1 = Me, R2 = Me; Cr4: R1 = Me, R2 = Bn), were synthesized and characterized by IR spectroscopy and elemental analysis. The studies of the solid state of Cr2 and Cr3 revealed distorted octahedral geometries around the chromium centers by X-ray diffractions. In the presence of MAO, Cr1–Cr4 exhibited high activities toward ethylene oligomerization (up to 2.17 × 106 g·mol–1(Cr)·h–1) and ethylene polymerization (up to 6.78 × 105 g·mol–1(Cr)·h–1). The oligomers were produced with high selectivity for α-olefins (>99%), confirmed by FT-IR and 13C NMR, and the distributions followed the Schulz–Flory equation. Various reaction parameters including the amount of cocatalyst, reaction temperature, and time were evaluated in detail, and it was evident that the title complexes had good thermal stability and the substituents on imidazole-N of the ligands dramatically impacted the catalytic activities as well as the distribution of the products.
研究领域:
化学
·
材料科学
✓ 已验证
DOI
高影响因子
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
·
2019
·
IF 2.606
Baolong Niu,Jie Guo,Xia Guo,Xiaoqing Sun,Chaohui Rao,Chun Liu,Jin Zhang,Ce Zhang,Yan-Ying Fan,Wenfeng Li
Although much effort has been focused on the preparation of stable amorphous calcium phosphate (ACP) nanoparticles , the poor dispersity of ACP nanoparticles in aqueous solution remain an unresolved problem. In this work, hexametaphosphate ((NaPO 3 ) 6 ) was used as a green dispersant to disperse casein-stabilized ACP (casein-ACP) nanoparticles in aqueous solution. Characterization of casein-ACP nanoparticles by X-ray diffraction (XRD) proved that the casein-induced amorphous structures could maintained for at least 3 months at room temperature. Moreover, the effects of (NaPO 3 ) 6 solutions for dispersing casein-ACP nanoparticles in water was studied and their ability to stabilize aqueous casein-ACP dispersions was demonstrated by digital photos. A typical anticancer drug , curcumin (Cur), was employed to evaluate the drug loading abilities and drug release behaviors of prepared nanoparticles. The results showed that the resultants have high drug loading capacity and favorable pH-responsive drug release properties. Furthermore, this drug delivery system showed an excellent ability to damage A549 cells, indicated that the as-prepared casein-ACP-(NaPO 3 ) 6 nanoparticles are promising for the applications in drug delivery.
研究领域:
材料科学
·
生物医学工程
·
药学
✓ 已验证
DOI
高影响因子
Polymer Chemistry
·
2023
·
IF 4.6
Tong Zhou,Yu-Ting Guo,Chun Yang,Xian-Bin Meng,Fusheng Du,Zichen Li
A new bicyclic lactone, 2,5,7-trioxabicyclo[2.2.2]octan-6-one (TOB), was synthesized by direct condensation reaction of glycerol and glyoxylic acid. The ring-opening polymerization (ROP) of TOB and its copolymerization with lactide (LA) under different conditions were systematically investigated. Both acid and base could catalyze the ROP of TOB, but only oligomers were obtained in low yields. Three discrete oligomers (O1, O2, and O3) of TOB were separated and characterized, confirming that the high crystallinity and poor solubility of PTOB in the polymerization media caused the low monomer conversion and low molar mass of PTOB. Copolymerization of LA and TOB was investigated with benzyl alcohol as the initiator and 1,5,7-triazabicyclo[4.4.0]dec-5-ene (TBD) as the catalyst. It was confirmed that LA was consumed very quickly to form PLA, followed by slow incorporation of TOB into the formed PLA chains. By varying the feed ratios of LA to TOB, a series of random copolymers with the incorporation ratio of TOB up to 49 mol% were obtained. These copolymers exhibited high glass transition temperatures (Tg) up to 100 oC. The TBD-catalyzed copolymerization mechanism was revealed to be a base-catalyzed transesterification of PLA and TOB. Finally, PLA-co-PTOB with variable Tg could thus be simply prepared by direct transesterification of PLA with TOB in the presence of TBD.
研究领域:
材料科学
·
高分子化学
✓ 已验证
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2026
·
IF 4
Ruizhi Tang,Shujuan Ma,Quan Bai,Yangyang Bian,Zichao Tang,Junjie Ou
Supramolecular affinity chromatography based on host-guest interactions represents a promising approach for achieving highly specific separation and purification of biomacromolecules. Among the various macrocyclic molecules, cucurbit[n]urils (CB[n]) have attracted significant attention in recent years due to their unique molecular recognition capabilities. Notably, recent studies have revealed the specific binding of CB[n] to aromatic amino acids and short peptides with aromatic residues or N-terminal motif. In this work, we report the specific recognition of protein phosphorylation sites by cucurbit[6]uril (CB[6]), and propose a CB[6]-based supramolecular affinity chromatography (CSAC) strategy for the enrichment of multi-phosphopeptides. A polymeric monolith was prepared by polymerization of perallyloxyl CB[6] and ethylene dimethacrylate. In order to study the recognition mechanism of CB[6] on phosphate groups, the chromatographic conditions of CSAC, including loading, washing and elution, were separately evaluated utilizing the CB[6] monolithic material as a platform. Molecular recognition of phosphorylation sites was achieved in the solution of high organic solvent content (90% ACN), which was rarely reported. The established method exhibited specific recognition on multi-phosphopeptides and was employed for enrichment of multi-phosphopeptides, which showed good specificity (1:1000 of β-casein: BSA). Furthermore, CSAC can effectively isolate multi-phosphopeptides from mouse liver and improve the coverage of phosphopeptides. The recognition of phosphate groups by CB[6] is observed in a high-organic solvent environment, which is distinctly different from the recognition effect previously reported in aqueous phases. The established CSAC strategy holds promise as an effective technique for biological separations.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）