Frontiers in Pharmacology
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2026
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IF 5.4
Jinku Guo,Yingfeng Cao,Kaipen Jin,Bing Liu,Wei Wang,Chen Chen,Jun Xie,Ankai Xu
BackgroundCyclovirobuxine D (CVB-D), an alkaloid from Buxus microphylla, exhibits anticancer effects in various tumors, but its role in osteosarcoma remains unexplored. This study investigates its efficacy and mechanism in osteosarcoma.MethodsWe screened a drug library and evaluated CVB-D’s effects on osteosarcoma cell lines using CCK-8 and colony formation assays. Saos2 and K7M2 cells were selected for further analysis. Flow cytometry assessed apoptosis and cell cycle. RNA-seq identified downstream pathways, including NF-kappaB, and stemness markers (CD24, ALDH1A1). Stemness was examined via serum-free suspension culture, and NF-kappaB pathway activator Diprovocim was used in rescue experiments. A xenograft mouse model validated the findings.ResultsCVB-D suppressed proliferation, stemness, and induced apoptosis in osteosarcoma. These effects may be partially mediated through the p-NF-kappaB2/NF-kappaB2 axis and were reversible upon NF-kappaB pathway activation.ConclusionCVB-D inhibits osteosarcoma possibly via the non-canonical NF-kappaB pathway, suggesting a potential therapeutic strategy.
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INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
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2026
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IF 5
Lihe Zhang,Yongjuan Li,Yuhang Bao,Jie Feng,Runyu Chen,Wenjing Zhang,Yixuan Zhu,Liwen Zhang,Yin Zhang,Feng Liang,Huimin Chen,Xinhua Luo,Guoqiang Tan,Wu Wang
D-Cycloserine (DCS) is a broad-spectrum antibiotic used to treat drug-resistant tuberculosis. It exerts its antimicrobial effects by inhibiting a variety of pyridoxal 5′-phosphate (PLP)-dependent enzymes. Recent studies indicate that cycloserine targets cysteine desulfurases, which are essential for iron–sulfur (Fe–S) cluster biogenesis. However, the mechanistic details of its effect on Fe–S cluster assembly are unclear. In this study, we demonstrate that L-Cycloserine (LCS), but not its enantiomer DCS, inhibits the cysteine desulfurase activity of Escherichia coli ( E. coli ) IscS, resulting in impaired Fe–S cluster biosynthesis. Liquid chromatography-mass spectrometry (LC–MS) analysis showed that LCS converts the IscS-bound PLP cofactor into pyridoxamine 5′-phosphate (PMP). Importantly, LCS significantly inhibited the growth of the uropathogenic E. coli strain CFT073, and remained effective against experimentally-evolved DCS-resistant strains in synthetic human urine medium. Our work highlights Fe–S cluster biosynthesis as a novel antimicrobial target and proposes LCS as a promising alternative therapeutic agent to counteract DCS resistance.
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CHEMICAL ENGINEERING JOURNAL
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2026
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IF 12.5
Liangmin Ning,Shuai Li,Shiyan Sheng,Jiuheng Ma,Jiawen Wang,Tianyu Zhang,Shun Wang,Jingyi Han,Jiaoyang Wang,Wei Chen,Meng Gao,Kunhua Wang,Min Fu,Hao Yu
Addressing the key challenges of controllable hydrogen transfer and directional C O bond activation in Meerwein-Ponndorf-Verley (MPV) reactions, this study constructed a Pt-CuO/Al 2 O 3 catalyst featuring Pt δ- Lewis acid-metal pairs for selective hydrogenation of biomass-derived furfural via the MPV reduction pathway. Under optimized conditions, the catalyst achieved 99.9% conversion with 98.0% selectivity. Density functional theory (DFT) analysis revealed that compared to isolated Pt, the d -band center of Pt in Pt-CuO/Al 2 O 3 shifts downward by 0.14 eV, while the d -band center of Cu shifts upward by 0.28 eV. Combined with the distinct metal-support interactions observed in EXAFS spectra, these results confirm that d -band center modulation creates a synergistic electronic structure of Pt δ– Cu δ+ : the upshifted d -band center of Cu δ+ enhances oxygen affinity, promoting co-adsorption of carbonyl and hydroxyl groups and facilitating six-membered ring transition state formation, while the downshifted d -band center of Pt δ- optimizes hydrogen transfer through interfacial electron transfer (Cu → Pt). These findings demonstrate the stable coordination environment of these Lewis acid-metal pairs. This work reveals the synergistic mechanism of Pt δ– Cu δ+ active sites and elucidates the cooperative “metal electron enrichment-oxide electron deficiency” effect in MPV reactions, providing new insights for designing highly efficient and selective biomass hydrogenation catalysts.
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POLYMER
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2026
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IF 4.5
Xianyu Guo,Wentao Tan,Ling Wang,Fengrui Yang,Yuhang Ma,Yi Shen,Caihong Luo,Qian Luo,Xiao Xie,Hui Li
Chiral separation of D- and L-Serine is of great value in the fields of biopharmaceuticals. For this purpose, a D-Serine imprinted polymer was prepared by precipitation polymerization with the aid of deep eutectic solvent (DES) and crowding agent (polymethyl methacrylate, PMMA). The preparation conditions were optimized by a L 9 (3 4 ) orthogonal design. Structure characterization was performed by the FTIR, SEM, AFM and XPS techniques and adsorption selectivity and separation efficiency for this imprinted material investigated. The results showed that under the conditions of pH 5.0 and 303 K, the polymer exhibited maximum adsorption capacity, reaching 121.4 mg g -1 , with a selectivity factor as high as 11.87. When this D-serine-imprinted material was used as a chromatographic stationary phase to separate a mixture of D- and L-serine, the resolution achieved was 1.765, demonstrating high chiral separation efficiency. The high adsorption selectivity for the novel imprinted material might result from the synergistic effect of deep eutectic solvent with macromolecular crowding reagent during the preparation of the imprinted materials. One hand, deep eutectic solvent can stabilize the zwitterionic form of D-Serine ( + H 3 N-CH(CH 2 OH)-COO - ) and hence enhance the ionic interactions between the template and functional monomers and the stability of template-functional monomer complexes; on the other hand, crowding agents significantly improved the binding efficiency between the functional monomer and the template molecules during the pre-assembly stage through the "excluded volume effect". This synergy promotes to form higher density and more precise specific recognition sites during the synthesis of the imprinted polymer and to improve the adsorption selectivity of the material obtained.
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Journal of Macromolecular Science Part A-Pure and Applied Chemistry
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2017
·
IF 0.963
Ranhong Li,Ling Li,Xiaotong Sun,Yan Wang
Dummy-template molecularly imprinted microspheres were synthesized via precipitation polymerization employing 2,4-D isooctyl ester as the template molecule instead of 2,4-D butyl ester, while methacrylic acid and divinylbenzene were used as functional monomer and cross-linker in acetonitrile or a mixture of acetonitrile and toluene. The microspheres were characterized by scanning electron microscopy, laser particle size analyzer and fourier transform infrared spectrometry. Binding capacity experiment showed that the molecularly imprinted polymers prepared in a mixture of acetonitrile and toluene had a high binding capacity. The performance of microspheres was further assessed by equilibrium binding and kinetic adsorption experiments. The results showed that the apparent maximum adsorption reached up to 1.35 mg·g−1 within 10 min. Based on the dummy-template microspheres, a molecularly imprinted solid phase extraction-gas chromatography method was developed for the selective analysis of 2,4-D butyl ester in soil samples. The mean recoveries of 2,4-D butyl ester from blank soil samples ranged from 85.9 to 99.3% with relative standard deviations of 4.5–14.3% (n = 5). The limit of detection and the limit of quantification of 2,4-D butyl ester were 0.8 μg·kg−1 and 2.3 μg·kg−1, respectively.
研究领域:
环境化学
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分析化学
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高分子科学
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农业化学
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Materials Science & Engineering C-Materials for Biological Applications
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2017
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IF 4.164
Guihua Cui,Yu Bai,Wenliang Li,Zhengguo Gao,Shuiying Chen,Nannan Qiu,Toshifumi Satoh,Toyoji Kakuchi,Qian Duan
A series of chain-end functional polymers composed of poly( N -isopropylacrylamide) (PNIPAM) and 2-amino-2-deoxy- d -glucopyranose( d -glucosamine, GA) was synthesized via atom transfer radical polymerization (ATRP). Novel fluorescent complexes of glucosamine-PNI- PAM/Eu(III) were then formed by chelation of the polymers and europium(III) ions. The aqueous solutions of the polymers and its Eu(III) complexes exhibited a lower critical solution temperatures (LCSTs), and which were approximately equal to body temperature. Cell viability assays suggested that these thermosensitive polymers and Eu(III) complexes showed excellent biocompatibility in vitro.
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ChemSusChem
·
2019
·Vol. 12
·期 24
·5274-5281
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IF 7.804
Lulu Jia,Hao Wan,Xiaohe Liu,Gen Chen,Ning Zhang,Junhui Li,Wei Zhou,Yijun Cao,Renzhi Ma,Guanzhou Qiu
Morphology and composition tuning of layered materials is evaluated to influence their electrochemical performance for energy storage and conversion applications. Layered Co 1− x Ni x hydroxides ( x= 0, 1/2, 1/3, 1/4, 1) of three different morphologies—nanocones, 2 D nanosheets obtained by the rapid exfoliation of nanoconical counterparts, and 2 D superlattice-like nanostructures alternately restacked by the oppositely charged hydroxide and graphene oxide (GO) nanosheets—have been systematically investigated for electrocatalytic oxygen oxidation. High activity is obtained with the 2 D Co 2/3 Ni 1/3 hydroxide nanosheets/GO superlattice (Co 2/3 Ni 1/3 NS–GO), achieving a current density of 10 mA cm −2 at a low overpotential of 259 mV accompanied by a small Tafel slope of 35.7 mV dec −1 , surpassing nanocones and 2 D nanosheets, as well as the congeneric heterostructured Co 1− x Ni x hydroxide nanosheets/GO nanoarchitectures (Co 1− x Ni x NS–GO; x= 0, 1/2, 1/4, 1) and the commercial RuO 2 electrocatalyst. The outstanding activity of Co 2/3 Ni 1/3 NS–GO superlattice uncovers the combined merits of 2 D superlattice-like structure and composition optimization for electrocatalysis, providing a strategy for developing high-performance electrochemical materials by rational morphology and composition design. Graphical A super lattice : Composition and morphology optimization was used to develop a 2 D Co 2/3 Ni 1/3 hydroxide/graphene oxide superlattice with high activity for oxygen evolution, achieving a current density of 10 mA cm −2 at a low overpotential of 259 mV accompanied by a small Tafel slope of 35.7 mV dec −1 . The outstanding activity of the superlattice highlights the importance of morphology and composition optimization for electrocatalysis.
研究领域:
材料科学
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电化学
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能量转换
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纳米材料
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催化
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ACTA PHYSICO-CHIMICA SINICA
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2026
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IF 13.5
Baogang Fu,Nan Su,Jianmin Luo,Yun Wang,Bo Hu,Liang Cheng,Hongjun Dong,Chunmei Li
SCN-AP with a D-π-A network was synthesized via sulfur doping and pyridine grafting. • SCN-AP achieves integrated design of 1 O 2 generation and kinetic matching of ORR/WOR. • SCN-AP boosts ·O 2 - / 1 O 2 generation, with a H 2 O 2 yield of 1030 μmol g -1 within 2 h. • Antibacterial assays verify the photoactivated antibacterial potential of SCN-AP. • In-situ XPS and DRIFTS characterize photogenerated electron flow and reaction intermediates. Photocatalytic H 2 O 2 synthesis is commonly dominated by the oxygen reduction reaction (ORR), whereas simultaneously enabling water oxidation reaction (WOR) and directional charge separation through band-structure regulation remains challenging. Herein, sulfur doping and pyridine modification were integrated to construct an intramolecular donor- π -acceptor (D- π -A) network within the carbon nitride framework. The resulting electronic polarization promotes directional separation of photogenerated charge carriers and optimizes the band structure to thermodynamically enable both ORR and WOR pathways. Consequently, the as-synthesized SCN-AP photocatalyst achieves an H 2 O 2 yield of 1030 μmol g -1 within 2 h in pure water, 3.73 times that of pristine PCN, and exhibits effective antibacterial activity against clinically relevant pathogens including Acinetobacter beijerinckii and Klebsiella variicola . The in-situ electron paramagnetic resonance (EPR) spectroscopy and in-situ diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS) collectively support the coexistence of ORR and WOR pathways. The ORR serves as the predominant route and involves a ·O 2 - -mediated stepwise reduction pathway together with a 1 O 2 -associated pathway, while WOR provides a complementary hole-driven contribution to H 2 O 2 formation. This work demonstrates a feasible band-engineering strategy for constructing polymeric photocatalysts with directional charge transfer and dual-pathway H 2 O 2 photosynthesis. This work demonstrates a D-π-A-mediated electronic polarization strategy that directs charge migration in carbon nitride, enabling dual-pathway H 2 O 2 photosynthesis through coupled ORR and WOR processes. Download: Download high-res image (411KB) Download: Download full-size image
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Microbial Cell Factories
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2026
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IF 5.8
Lin Menghui,Zheng Miao,Chen Kaiyi,Xu Xinyu,Chen Li,Guo Xiaogang,Le Thanh Ninh,Zhao Minjie,Feng Yanru,Cai Haiying
Heat stress is prevalent in industrial biomanufacturing processes including Baijiu brewing and bioethanol production. It disrupts the membrane structure and lipid homeostasis of Saccharomyces cerevisiae , interferes with genome-wide gene transcription and retards cell growth, thereby greatly lowering fermentation efficiency and product yields. There is an urgent demand for food-safe additives to alleviate heat-induced damage to industrial yeast. Although vitamin D₂ (VD₂) shares a similar sterol structure with endogenous yeast ergosterol, its regulatory effects on yeast under heat stress remain poorly understood. Here, we adopted an integrated strategy combining phenotypic characterization, lipidomics, transcriptomics and molecular docking to explore the protective effects and regulatory pathways of exogenous VD₂ against heat stress in S. cerevisiae . Phenotypic assays showed that VD₂ supplementation effectively alleviated heat-induced growth retardation, elevated intracellular glutathione peroxidase activity and reduced membrane injury. Lipidomic analysis revealed that VD₂ remodelled cellular lipid profiles by increasing triglyceride levels, decreasing phosphatidylcholine content and promoting sphingolipid synthesis. Transcriptomic results further demonstrated significant upregulation of key genes involved in the S-adenosylmethionine (SAM) metabolic axis. Integrated multi-omics data and molecular docking prediction support a tentative mechanistic hypothesis: VD₂ may interact with Opi3p and potentially interfere with its catalytic function, which could further drive downstream metabolic reprogramming. In conclusion, this study reveals that VD₂ alleviates heat-triggered growth suppression by remodelling the SAM-dependent lipid metabolic network. As a food-grade additive, VD₂ possesses promising potential to alleviate heat-induced growth retardation of yeast starters in industrial fermentation. Our findings provide a theoretical basis for developing natural additives to mitigate yeast heat damage and optimizing high-temperature fermentation bioprocesses.
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Small
·
2026
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IF 11.8
Runren Jiang,Hanqi Yue,Yuyue Liu,Yilin Deng
Developing efficient water purification technologies without external chemical inputs is critical for treating emerging contaminants. Herein, we report a Cu 2 WS 4 -CoPc composite piezocatalyst that enables efficient in situ H 2 O 2 production and pollutant degradation via enhanced oxygen activation. The introduction of CoPc induces interfacial electron transfer from Cu 2 WS 4 to CoPc and promotes O 2 activation through d-p orbital hybridization between Co and O 2 , as confirmed by density functional theory calculations showing a decrease in O 2 adsorption energy from +1.2 to −0.3 eV. Consequently, the composite exhibits significantly enhanced piezoelectric response, with increased piezoelectric constant and effective longitudinal piezoelectric coefficient. The optimized Cu 2 WS 4 -CoPc generates H 2 O 2 via a two-electron oxygen reduction pathway, with superoxide radicals (·O 2 − ) as the key intermediate, achieving a yield of 183.4 µM within 30 min, which far exceeds that of pristine Cu 2 WS 4 . Moreover, the catalyst degrades ciprofloxacin with a rate constant of 0.059 min −1 , approximately 4.5 times higher than pure Cu 2 WS 4 , and zebrafish toxicity assays confirm effective detoxification of degradation intermediates. This study provides a new strategy for enhancing piezocatalytic H 2 O 2 synthesis through strengthened oxygen activation and lays a theoretical foundation for designing efficient, environmentally benign water treatment technologies.
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JOURNAL OF ELECTROANALYTICAL CHEMISTRY
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2026
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IF 4.6
Xiaolong Ma,Jiaxue Huang,Maolin Ran,Yuzhu Yang,Biao Qin
IrFeCoNiMo HEA catalyst with multi-d-orbital hybridization is developed for alkaline OER. • IrFeCoNiMo shows 249 mV overpotential at 10 mA cm⁻² and 50.26 mV dec⁻¹ Tafel slope in 1 M KOH. • IrFeCoNiMo exhibits 32.2-fold higher TOF than IrO₂, with low charge transfer resistance and large surface area. • IrFeCoNiMo shows exceptional OER stability with only 1.2% overpotential decay after 5000 CV cycles and 40 h test. • Multi-d-orbital hybridization and HEA lattice distortion synergistically enhance OER performance. To address the bottlenecks of low catalytic efficiency and insufficient stability in the oxygen evolution reaction (OER) for water electrolysis hydrogen production, a multi-d-orbital synergistic modulation strategy is proposed, and the IrFeCoNiMo high-entropy alloy catalyst is fabricated via a solvothermal method. Composed of transition metals with distinct d-orbitals, the catalyst exhibits a single face-centered cubic (FCC) structure, uniform nanomorphology, and the coexistence of multiple valence states of metal elements. In alkaline electrolyte, it delivers a low overpotential of 249 mV at 10 mA cm −2 , a small Tafel slope of 50.26 mV dec −1 , and a turnover frequency (TOF) value 32.24 times higher than that of commercial IrO₂. Moreover, the catalyst demonstrates excellent stability after 5000 cyclic voltammetry (CV) cycles and a 40-h chronopotentiometry test. Mechanistic analysis confirms that the orbital hybridization induced by directional electron transfer among multiple d-electron orbitals, combined with the lattice distortion effect of high-entropy alloys, optimizes intermediate adsorption, increases active sites, and accelerates charge transfer. This study provides a new pathway for the design and development of high-performance alkaline OER catalysts, which is of great significance for promoting the large-scale application of water electrolysis hydrogen production technology. IrFeCoNiMo high-entropy alloy with multi-d-orbital hybridization is fabricated via solvothermal method. It exhibits ultrahigh alkaline OER activity (249 mV@10 mA cm −2 ) and exceptional stability, with TOF 32.24 times that of commercial IrO₂, attributed to optimized intermediate adsorption and accelerated charge transfer from orbital synergy and lattice distortion. Download: Download high-res image (87KB) Download: Download full-size image
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INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2026
·Vol. 65
·期 31
·16632-16643
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IF 3.9
Huang Heng,Yin ChunYu,Xia Tao,Zhou Yebin,Wan Dongchuang,Zhu Xiaofeng,Jiang Kaijun,Hu Rongrui,Xiang Zhenli,Ma Chaofan,Li Xiaonian,Lu Chunshan
Chlorinated volatile organic compounds (CVOCs) hydrodechlorination
is hindered by chlorine poisoning and the instability of monometallic
catalysts, limiting their practical application. In this work, long-range
ordered PdCu intermetallic compounds were synthesized via a microwave-assisted
method. Characterization confirmed that microwave treatment induced
strong Pd–Cu electronic interactions and lattice ordering,
thereby facilitating the formation of intermetallic phases. Catalytic
evaluations demonstrated that the PdCu/C-Ordered catalyst achieved
an ethane production rate of 4.47 mmol·g cat –1 ·h –1 at 300 ° C with an apparent activation
energy of 31.28 kJ/mol, while maintaining exceptional stability over
a 300 h continuous operation. Density functional theory (DFT) calculations
further elucidated that the intermetallic phase, characterized by
a d-band center positioned closer to the Fermi level, exhibited enhanced
reactant adsorption and activation capabilities. Concurrently, it
significantly lowered the HCl desorption energy barrier, thereby effectively
mitigating chlorine poisoning effects. This research provides a novel
pathway for the design of high-efficiency hydrodechlorination catalysts
based on the precise regulation of electronic structures.
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