CHEMISTRY-A EUROPEAN JOURNAL
·
2025
·
IF 3.7
Zheng Liu,Jun-Jie Fang,Zhi-Yi Wang,Yun-Peng Xie,Xing Lu
Graphical The inclusion of carboxylic acid ligands significantly impacts the structural arrangement of the metal core in Cu(I) alkynyl clusters, thereby affecting their fluorescence properties. Furthermore, the incorporation of ferrocene into these clusters has been noted to induce modifications in the electrochemical performance of the Cu(I) alkynyl clusters. Surface ligands play a pivotal role in the functional design of atomically precise Cu(I) nanoclusters. They act as protective agents, ensuring the stability of the Cu(I) clusters, while also influencing their structure and properties. This study delves into the synthesis, structure, and properties of innovative Cu(I) nanoclusters co-stabilized by carboxylic acid and alkynyl ligands. The findings reveal that these surface ligands wield a significant impact on the cluster's structure and can even modulate the luminescent characteristics of the Cu(I) alkynyl clusters. Moreover, density-functional theory (DFT) calculations shed light on how different carboxylic acid ligands affect the UV-visible absorptivity of paired Cu(I) clusters. In addition, ferrocene carboxylic acids were employed as protective ligands to impart electrochemical properties to the Cu(I) clusters. This research presents an effective methodology for synthesizing atomically precise Cu(I) clusters shielded by carboxylic acid ligands.
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PubMed
高影响因子
Cell Discovery
·
2025
·Vol. 11
·期 1
·1-14
·
IF 12.5
Wang Jinrui,Niu Shenghui,Hu Xiao,Li Tianxing,Liu Shengduo,Tu Yingfeng,Shang Zehua,Zhao Lin,Xu Pinglong,Lin Jingwen,Chen Lu,Billadeau Daniel D.,Jia Da
The cGAS-STING pathway mediates the innate immune response to cytosolic DNA, contributing to surveillance against microbial invasion or cellular damage. Once activated, STING recruits TBK1 at the trans-Golgi network (TGN), which in turn phosphorylates IRF3 to induce type I interferon (IFN-I) expression. In contrast to STING, little is known about how TBK1 is transported to the TGN for activation. Here, we show that multiple TGN tethering factors, a group of proteins involved in vesicle capturing, are indispensable for STING-IFN-I signaling. Deletion of TBC1D23, a recently reported tethering factor, in mice impairs the STING-IFN-I signaling, but with insignificant effect on STING-NF-κB signaling. Mechanistically, TBC1D23 interacts with TBK1 via the WASH complex subunit FAM21 and promotes its endosome-to-TGN translocation. Furthermore, multiple TGN tethering factors were reduced in aged mice and senescent fibroblasts. In summary, our study uncovers that TGN tethering factors are key regulators of the STING-IFN-I signaling and suggests that their reduction in senescence may produce aberrant STING signaling.
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高影响因子
Food & Function
·
2015
·Vol. 7
·期 1
·409-416
·
IF 2.791
Kaige Ji,Yudan Zhao,Tianhong Yu,Zhuoyi Wang,Hao Gong,Xin Yang,Yang Liu,Kun Huang
Parkinson's disease (PD) is one of the most common neurodegenerative diseases. Lewy bodies that are formed by the aggregated α-synuclein are a major pathological feature of PD. Salvia miltiorrhiza has been used as food and as a traditional medicine for centuries in China, with tanshinone I (TAN I) and tanshinone IIA (TAN IIA) as its major bioactive ingredients. Here, we investigated the effects of TAN I and TAN IIA on α-synuclein aggregation both in vitro and in a transgenic Caenorhabditis elegans PD model (NL5901). We demonstrated that TAN I and TAN IIA inhibited the aggregation of α-synuclein as demonstrated by the prolonged lag time and the reduced thioflavin-T fluorescence intensity; TAN I and TAN IIA also disaggregated preformed mature fibrils in vitro. Moreover, the presence of TAN I or TAN IIA affected the secondary structural transformation of α-synuclein from unstructured coils to β-sheets, and alleviated the membrane disruption caused by aggregated α-synuclein in vitro. Besides, the immuno-dot-blot assay indicated that TAN I and TAN IIA reduce the formation of oligomers and fibrils. We further found that TAN I and TAN IIA extended the life span of NL5901, a strain of transgenic C. elegans that expresses human α-synuclein, possibly by attenuating the aggregation of α-synuclein. Taken together, our results suggested that TAN I and TAN IIA may be explored further as potential candidates for the prevention and treatment of PD.
研究领域:
神经科学
·
药理学
·
分子生物学
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PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2019
·
IF 3.858
Fei Li,Jie Gao,XiaoXuan Li,Yijun Li,Xiwen He,Langxing Chen,Yukui Zhang
In this work, we presented the preparation of magnetic carbon nanotubes (MCNTs) functionalized with molecularly imprinted polymers (MIPs) for effective removal of aristolochic acid I (AAI) in traditional Chinese medicine (TCM). MCNTs@AAI-MIPs was obtained via a facile and environmental friendly sol-gel process. Firstly, MCNTs were synthesized by a solvothermal method. Then, the template molecules were self-assembled with the functional monomer phenyltrimethoxysilane (PTMOS) in the presence of ethanol and water. Finally, AAI-MIPs film was coated on the MCNTs to obtain product MCNTs@AAI-MIPs using tetraethyl-orthosilicate (TEOS) as cross-linker. The morphology and structure of prepared MIPs were characterized by Fourier transform infrared spectroscopy (FT-IR), transmission electron microscopy (TEM), X-ray diffraction (XRD), nitrogen gas adsorption and vibrating sample magnetometer (VSM). The adsorption properties were demonstrated by kinetic, isothermal and selective adsorption experiments. The results showed that the imprinted nanocomposites exhibited fast separation rate (10 s), high adsorption capacity (18.54 μg∙mg −1 ), short kinetic equilibrium time (15 min), and good selectivity to template molecule with imprinting factor (IF) of 3.17. A regression equation (y=57294x-4734.1) with good linearity was obtained in the concentration range of 0.1–200 μg∙mg −1 for AAI with a correlation coefficient (R 2 ) of 0.9998. The limit of detection (LOD, S/N=3) was 0.034 μg∙mg -1 . Moreover, high recoveries ranged from 80% to 110% (RSD=3.27%-8.16%) were received in spiked TCM samples. The results suggested that the proposed MCNTs@AAI-MIPs could efficiently and specifically capture AAI from an actual complex TCM samples.
研究领域:
材料科学
·
环境科学
·
药物分析
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高影响因子
ANALYTICAL CHEMISTRY
·
2021
·
IF 6.986
Xianlong Zhang,Yongning Wu,Juhong Chen,Yan Yang,Guoliang Li
The copper(I)-catalyzed azide–alkyne cycloaddition (CuAAC) click reaction has drawn increasing attention in the field of analytical science. However, the poor stability of Cu(I) usually hinders not only the simplicity of the click reaction but also its applications in precise analyses. Therefore, the development of a nanocatalyst containing stable Cu(I) is of great significance for broadening the application of CuAAC-based assays. Herein, inspired by the active center structure of natural multicopper oxidases (MCOs), we successfully prepared a novel nanocatalyst containing abundant stable Cu(I) as an artificial “clickase” (namely, CCN) by using glutathione to stabilize Cu(I). The stability and enzyme-like catalytic activity in the CuAAC reaction of the prepared CCN clickase were studied, and the catalytic mechanism of the CCN clickase-mediated CuAAC reaction between 3-azide-7-hydroxycoumarin (Azide 1) and propargyl alcohol (Alkyne 2) was also revealed. Compared with the existing solid CuO nanocatalysts used in CuAAC-based assays, CCN clickases exhibited plenty of superior properties (including high stability, excellent catalytic activity, no requirements of dissolution and reducing agents/radical initiator during the detection, well-defined porosities benefiting the substrate diffusion, and good biocompatibility), which can greatly increase the reaction efficiency and shorten the detection time. Encouraged by these remarkable performances, CCN clickases were used as labels to establish a new catalytic click fluorescence immunoassay for foodborne pathogens. Notably, the proposed CCN clickase-based immunoassay exhibited high analytical performances for the quantification of Salmonella enteritidis in the linear range of 102–106 CFU/mL with a limit of detection as low as 11 CFU/mL. The developed method has also been used in the determination of S. enteritidis in food samples, showing its great potential in the detection of foodborne pathogens.
研究领域:
分析化学
·
纳米技术
·
免疫学
·
食品安全
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PubMed
高影响因子
JOURNAL OF ALLOYS AND COMPOUNDS
·
2020
·
IF 4.65
Jiangping Jing,Zhuoyuan Chen,Chang Feng,Mengmeng Sun,Jian Hou
Photoelectrochemical cathodic protection is emerging as a green and environmental method to protect metals against corrosion. Graphitic carbon nitride (g-C 3 N 4 ) shows great application potential in this area because of its high efficiency, high stability and low cost. However, the pristine g-C 3 N 4 shows amphoteric properties, resulting in its failure in providing photoelectrochemical cathodic protection for the coupled metal in NaCl solution. In this work, a K&I co-doping technique was performed to modulate the band structure of g-C 3 N 4 , and an n-type g-C 3 N 4 (n-C 3 N 4 ) was obtained. The changes in the molecular structure were studied by SEM, HRTEM , XRD , EDS, XPS and FT-IR technologies. The n-type semiconductor characteristics were verified by the photoinduced i-V curves, SKP potential distributions and pH drift techniques. The n-C 3 N 4 can generate positive photocurrent in the whole investigated potential range, making it able to provide photoelectrochemical cathodic protection for the coupled 316L stainless steel in NaCl solution. This work is very helpful for promoting the application of g-C 3 N 4 in the fields of the photoelectric conversion and the photoelectrochemical cathodic protection.
研究领域:
材料科学
·
腐蚀与防护
·
电化学
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高影响因子
Advances in Experimental Medicine and Biology
·
2016
·
IF 1.953
Yan Heping,Chen Yashun,Zhang Jucheng,Liu Wei,Chen Rui
The first derivatives of gelatin and type I collagen fluorescence spectra were characterized in order to describe the effect of free radicals on pyridinoline (PYD) cross-links. The different gas saturation conditions were used to investigate the effect of different free radicals. An analysis of first derivative fluorescence spectra suggests that PYD cross-link fluorescence emission is composed of three peaks in gelatin, but only two in type I collagen. The PYD cross-link was photo-degraded more than other gases in the presence of O 2 . This suggests that the singlet oxygen ( 1 O 2 ) plays a key role when using photodynamic therapy to treat skin fibrosis disease with Hypocrellin B (HB).
研究领域:
光生物学
·
皮肤科学
·
分子生物学
·
结缔组织病
·
自由基化学
·
光动力学
·
纤维化疾病
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DOI
高影响因子
Advanced Sensor Research
·
2024
·
IF 0
Zhichao Yu,Di Wu,Yuan Gao,Yunsen Wang,Yongyi Zeng,Dianping Tang,Xiaolong Liu
Early intervention in acute myocardial infarction can minimize myocardial damage and improve patient survival. Herein, a low-cost device-free portable immunobiosensing platform for flexible monitoring of immediate myocardial infarction is reported. CuS-Pt nanofragments (CuS-Pt NFs) with high photothermal conversion efficiency (≈26.41%) are synthesized by liquid-phase polarity-mediated synthesis. The CuS NFs are loaded in situ with platinum (Pt) nanoreactors using a solvothermal reduction strategy, which is employed to enhance the efficiency of gas production. The resulting CuS-Pt nanocatalysts are encapsulated within liposomes for signal cascade amplification. Specifically, cardiac troponin I (cTn I), a target biomarker in serum, is captured on pre-modified microtiter plates and formed into a classical sandwich model. The thermo-chemically kinetically enhanced CuS-Pt reactor is released through a one-step chemical treatment and transferred to a closed gas generator. Under the excitation of a near-infrared laser emitter, the internal pressure in the gas generator device increases with time and drives the carbon quantum dot solution in the connected hose. The moving distance shows a correlation with the target concentration. This work provides a new implementation for the development of low-cost, efficient pressure immunosensors without the requirement of a readout device.
研究领域:
生物医学工程
·
心脏病学
·
纳米技术
·
生物传感技术
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DOI
Sensing and Bio-Sensing Research
·
2025
·
IF 4.9
Yue He,Xinyue Qu,Tingting Lan,Mingxing Yu,Xu Duan,Ruyi Chen,Junhao Wu,Zhihong Wang,Deling Kong,Zhongyang Shen,Shuang Yang,Chunlei Zhou,Tao Ming
As a key biomarker in clinical diagnostics, cardiac troponin I facilitates the early diagnosis of acute myocardial infarction. This study developed a rapid quantitative method for detecting cardiac troponin I in blood, aiming to establish its quantitative correlation with myocardial infarction area by accurately quantifying the serum cTnI concentration in patients suffering from acute myocardial infarction. We utilized a cTnI-specific aptamer as the recognition element. Gold nanoparticles exhibit salt-induced aggregation with visible color change. The aptamer protects gold nanoparticles from high-salt aggregation. Through systematic optimization, we established a gold nanoparticle-based colorimetric assay leveraging this anti-aggregation mechanism. Results demonstrated that under optimal conditions—200 mM NaCl and 800 nM aptamer concentration—the developed assay exhibited a promising linear response (R 2 = 0.942) across a cardiac troponin I concentration spanning from 0.1 to 0.8 μg/mL. According to the standard calibration method, the detection limit was estimated as 0.1 μg/mL. We observed recovery rates ranging from 105.6 % to 118.5 %. This research presents a highly sensitive, specific, and rapid point-of-care detection methodology for cardiac troponin I, offering enhanced field-testing efficiency and providing a low-cost alternative for serum cardiac troponin I detection. Moreover, it lays the groundwork for the development of a novel methodology enabling dynamic and quantitative assessment of infarct size in patients suffering from myocardial infarction.
研究领域:
生物医学工程
·
临床诊断
·
纳米生物技术
·
即时检测
·
心血管医学
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高影响因子
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
·
2025
·
IF 2.7
Yun Li,Ruijin Pu,Meng Su,Ruiqin Mao,Yajun Zheng,Zhiping Zhang
Methimazole (MMI), which is primarily known as an antithyroid drug, has received considerable attention for its potent antioxidant properties in a copper(I)-mediated Fenton reaction. However, the antioxidant mechanistic details of MMI, particularly its structural analogy to the natural antioxidant ergothioneine (both of which feature an imidazole-2-thione moiety), remain largely unexplored due to the difficulty of in situ characterizing unstable reaction intermediates. In this study, we combined an in situ reactor-integrated paper-in-tip spray ionization source with mass spectrometry (PTSI-MS) to probe the protection mechanisms of MMI against the CuI-catalyzed Fenton reaction online. We present direct MS evidence of the antioxidant mechanisms of MMI (M) in its reduced form (MSH) and oxidized disulfide form (MSSM). The former can directly sequester H2O2 (i.e., a H2O2 scavenging mechanism). The latter tightly coordinates with CuI to form a CuI-MSSM complex that stabilizes CuI and prevents its oxidation by H2O2 and thus the formation of •OH (i.e., a metal ion coordination mechanism). The proposed reaction pathway of the CuI complexes from MMI disulfide (CuI-MSSM, m/z = 289 and 291) to MMI thiosulfonate (CuI-MSO2SM, m/z = 321 and 323) to MMI monosulfide (CuI-MSM, m/z 257 and 259) was verified by S–S oxidation, SO2–S bond cleavage, and extruded sulfur elimination, which confirmed the stable N,N′-bidentate binding of CuI to these intermediates, even when attacked by H2O2. These findings challenge the traditional radical scavenging mechanism and contribute to our understanding of the relationship between the structure and antioxidant activity of imidazole-2-thiones. Furthermore, the established in situ reactor-integrated PTSI-MS approach provides unique insights into reaction dynamics, offering the advantages of simplicity, speed, and low reaction volume (down to 20 μL).
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高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Ruiyang Qin,Fanyue Meng,Lijian Leng,Zhengyong Xu,Hailong Li,Shaowei Zhang
Thallium (Tl) and its compounds pose significant environmental risks due to their high toxicity and bioaccumulative potential, with industrial discharge standards mandating Tl concentrations below 5 μg/L. This study addresses the underexplored challenge of removing trace Tl(I) (≤1 mg/L) by investigating the efficacy of zinc sulfide (ZnS) as an adsorbent. Batch experiments, complemented by kinetic analyses (pseudo-second-order model), isotherm studies (Langmuir model, Qmax = 34.7 mg/g), and multi-technique characterization, were conducted to evaluate adsorption performance. Results show that ZnS achieves >99.9 % removal of trace Tl(I) at pH 10, reducing effluent concentrations to <0.4 μg/L—well below regulatory limits. Mechanistic investigations reveal a synergistic pathway: electrostatic attraction to negatively charged hydroxylated ZnS surfaces (zeta potential = −15.4 mV at pH 10), surface complexation via ≡ Zn-O-Tl bonds, and gradual formation of crystalline Tl₂S precipitates over prolonged contact. A two-stage treatment strategy, integrating sodium sulfide precipitation with ZnS adsorption, effectively purifies real-world matrices, including Tl-contaminated rainwater (Tl(I) from 352 μg/L to 0.440 μg/L) and high-salt industrial effluent (Tl(I) from 55.0 mg/L to 0.330 μg/L). This work provides experimental evidence for ZnS as a viable adsorbent for trace Tl(I) remediation, supporting the development of cost-effective heavy metal removal technologies.
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高影响因子
FUEL PROCESSING TECHNOLOGY
·
2023
·
IF 7.5
Dingshan Cao,Cong Luo,Tong Luo,Zhaowei Shi,Fan Wu,Xiaoshan Li,Ying Zheng,Liqi Zhang
One of the research priorities in dry reforming of methane (DRM) technology is the development of highly active and stable catalysts. Using crystalline oxides as catalyst precursors is an effective catalyst optimization scheme for DRM. Therefore, the application of La 2 NiO 4 perovskite in DRM was systematically studied, including preparation, characterization, carbon deposition properties, regeneration properties, and B-site doping, etc. This paper is the first part, the effects of preparation methods (sol-gel, co-precipitation, hydro-thermal, and solid-state methods) on the physicochemical properties and DRM performance of La 2 NiO 4 samples were investigated, and the relationship between materials' structure and their catalytic performance was analyzed. The results showed that compared with the samples prepared by the hydro-thermal and co-precipitate methods, the sample prepared by the sol-gel method exhibited the highest conversion of CH 4 and CO 2 (91.4% and 91.5%, respectively) and the best carbon resistance in DRM, which were mainly attributed to its richer microstructure, higher reducibility and basicity . While the lowest DRM performance of the sample prepared by the solid-state method was mainly due to the small amount of reduced amorphous NiO and the weak reducibility of the perovskite phase in this sample, resulting in the inability of perovskite in-situ decomposition under the DRM atmosphere.
研究领域:
催化化学
·
材料科学
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高影响因子
危险品化学品经营许可证（带存储）