CHEMICAL ENGINEERING JOURNAL
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2024
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IF 13.3
Ying Xu,Genkuo Nie,Xiao Jiang,Hongyu Wang,Guihua Yang,Ziyi Yan,Ji-Jun Zou,Hailong Yu,Shitao Yu,Yu Liu
Metal-metal oxide catalysts are robust in selective hydrodeoxygenation of biomass derives. Herein, 1-hexanol in total yield about 76 % is firstly achieved by high selective hydrodeoxygenation of 5-hydroxymethylfurfural over Ni/MCM-41 and Pt-WO X /t-ZrO 2 . By controlling the amount ratio of Ni/Ni 2+ to 0.46, a 99.9 % yield of 2,5-tetrahydrofurandimethanol was achieved from 5-hydroxymethylfurfural by Ni/MCM-41. Subsequently high yield of 76 % of 1-hexanol was obtained by competing pathways of tandem reactions beginning at SN2 reaction at the epoxy atom of 2,5-tetrahydrofurandimethanol to enol/tautomer intermediates and ending with controllable selective hydrogenation. The reaction mechanism is explored and the reaction-oriented regulation is realized by the additional strong BrÖnsted acid sites generated by electron transfer from W to Pt and the appropriate amount of Pt 0 sites of Pt-WO X /t-ZrO 2 , where the ratios of W 5+ /W 6+ and Pt 0 /Pt 2+ are 0.31 and 4.58 respectively. This work provides a new and green method for the synthesis of 1-hexanol and expands the utilization of metal–metal oxide in making value-added products using sustainable feedstocks.
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Redox Biology
·
2024
·
IF 10.7
Jing Xu,Chang He,Yongsong Cai,Xipeng Wang,Jidong Yan,Jing Zhang,Fujun Zhang,Vilma Urbonaviciute,Yuanyuan Cheng,Shemin Lu,Rikard Holmdahl
Autoimmune diseases, such as rheumatoid arthritis (RA) and systemic lupus erythematous, are regulated by polymorphisms in genes contributing to the NOX2 complex. Mutations in both Ncf1 and Ncf4 affect development of arthritis in experimental models of RA, but the different regulatory pathways mediated by NOX2-derived reactive oxygen species (ROS) have not yet been clarified. Here we address the possibility that intracellular ROS, regulated by the NCF4 protein (earlier often denoted p40phox) which interacts with endosomal membranes, could play an important role in the oxidation of cysteine peptides in mononuclear phagocytic cells, thereby regulating antigen presentation and activation of arthritogenic T cells. To study the role of NCF4 we used mice with an amino acid replacing mutation (NCF4 R58A ), which is known to affect interaction with endosomal membranes, leading to decreased intracellular ROS production. To study the impact of NCF4 on T cell activation, we used the glucose phosphate isomerase peptide GPI 325-339 , which contains two cysteine residues (325-339c-c). Macrophages from mice with the NCF4 58A mutation efficiently presented the peptide when the two cysteines were intact and not crosslinked, leading to a strong arthritogenic T cell response. T cell priming occurred in the draining lymph nodes (LNs) within 8 days after immunization. Clodronate treatment, which depletes antigen-presenting mononuclear phagocytes, ameliorated arthritis severity, whereas treatment with FYT720, which traps activated T cells in LNs, prohibited arthritis. We conclude that NCF4-dependent intracellular ROS maintains cysteine peptides in an oxidized crosslinked state, which prevents presentation of peptides recognized by non-tolerized T cells and thereby protects against autoimmune arthritis.
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PubMed
高影响因子
ACS Nano
·
2025
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IF 16
Lei Huang,Xinbo Li,Hongyan Zhang,Feng Liu,Zheng Dai,Fang Xiao,Lin Wang,Zheng Wang
Peptide-based neoantigen vaccines are promising cancer immunotherapy strategies because of their capability to induce durable tumor-specific immune responses. However, insufficient neoantigen-specific T-lymphocyte activation greatly limits their clinical efficacy. Here, we developed sericin-coordinated zinc ion-modified calcium phosphate (CP) nanovaccines that codeliver tumor antigen peptides and a Toll-like receptor 9 agonist (SZCP/APs-CpG) for potentiating antigen-specific T cell immunity. SZCP/APs-CpG nanovaccines could yield efficient codelivery of antigen peptides and adjuvants to dendritic cells (DCs) in draining lymph nodes (dLNs), induce hyperactive DCs depending on the inflammasome-dependent interleukin-1β secretion, and coordinate the released Zn2+-induced T cell activation to elicit robust and durable antigen-specific T cell immune responses. Vaccination with SZCP/APs-CpG exhibited potent anticancer efficacy and superior safety in multiple murine cancer models and significantly protected against B16-OVA tumor rechallenge and eradicated orthotopic colon cancer in mice when combined with immune checkpoint blockade. Thus, our work presents an efficient and versatile nanovaccine platform for boosting antigen-specific T cell activation for cancer immunotherapy.
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PubMed
高影响因子
Journal of Functional Foods
·
2019
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IF 3.197
Jingyi Ma,Juan Zhang,Qiuhong Li,Zhaoling Shi,Huajie Wu,Huiqin Zhang,Liping Tang,Ru Yi,Hui Su,Xin Sun
Although the prevalence of food allergies is rising, the standard of care for these allergies is suboptimal. Probiotics have been reported to regulate the allergic immune response, but the underlying mechanisms are not fully understood. In this study, we aimed to evaluate the effect of a mixture of probiotics on food allergy in an ovalbumin (OVA)-induced murine model. Treatment with the probiotics attenuated OVA-induced allergic symptoms in mice. Flow cytometry analysis showed that oral administration of probiotics induced mucosal CD103 + dendritic cells (DCs), which promoted differentiation of regulatory T cells (Tregs). 16S rRNA analysis revealed that the probiotics modulated the composition of microbiota, especially by increasing the proportion of the Deferribacteres and Verrucomicrobia phyla and the Mucispirillum and Clostridium XlVa genera, which in turn regulated the immune system. Our findings provide molecular insight into the application of a mixture of probiotics to alleviate food allergies and even gut immune homeostasis.
研究领域:
免疫学
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微生物学
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营养学
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高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2022
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IF 8.025
Zhuohao Xie,Peng Li,Haonan Zhan,Liuyan Zhuang,Sijian Tao,Liehua Deng,Jiahao Huang
Efficient and accurate detection of melamine in dairy products remains a crucial yet challenging task. Herein, an allosterically modulated G-quadruplex-integrated aptamer is rationally designed with thymine-rich recognition termini for melamine binding. The detection process is facile by simply introducing the analyte into the mixture consisting of G-quadruplex aptamer probes, exonuclease III, and thioflavin T (ThT). The detection feasibility is confirmed by the polyacrylamide gel electrophoresis and fluorescence measurement results. This exonuclease III-assisted signal amplifiable approach works well in a linear range from 0.1 nM to 0.1 μM. Moreover, a detection limit as low as 83 pM is easily achieved, which is almost five orders of magnitude smaller than the maximum allowable melamine levels (about 8 μM) defined by many countries all over the world. The whole assay time for each test is no longer than 1 h. Additionally, the scheme is highly specific and satisfactory recovery rates (from 91% to 104%) are readily obtained when challenged with melamine-spiked milk samples. Therefore, the label-free, turn-on, low-cost, and time-efficient method can be used for reliable detection of melamine in an easily manipulated and ultra-sensitive manner, which may find its utilization in the field of food safety, biomedical engineering, and clinical diagnosis.
研究领域:
分析化学
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食品安全
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生物医学工程
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临床诊断
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DOI
PubMed
高影响因子
ADVANCED FUNCTIONAL MATERIALS
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2019
·Vol. 29
·期 12
·1806916
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IF 15.621
Xiaowei Liu,Yanlin Feng,Guangchao Xu,Yang Chen,Ya Luo,Jinen Song,Yu Bao,Jiqiao Yang,Chune Yu,Yanna Li,Haoyu Ye,Bowen Ke,Bo Chen,Jianping Hu,Jie Xu,Huan Meng,Haiyuan Zhang,Hubing Shi
The combination of BRAF/MEK-targeted therapy with immune checkpoint blockade is regarded as a promising regimen for patients with metastatic melanoma due to their complementary advantages. However, MEK-inhibitor-induced T-cell toxicity impedes effective cooperation. In this experiment, a pH-responsive on-demand controlled release mesoporous silica nanoparticles (MSNPs) system is designed. Fluorescein-isothiocyanate-loaded MSNP can be specifically delivered into tumor cells rather than T-cells. MEK-inhibitor-loaded MSNP avoids proliferative and functional inhibitions of T-cells, while preserving growth suppression of tumor cells in vitro. In an in vivo model, MSNP encapsulation reverses the MEK-inhibitor-induced suppression of activated CD8 + T-cells, and enhances the secretion of INF-γ and IL-2. The combination of BRAF inhibitor plus MSNP-loaded MEK inhibitor and anti-PD-1 antibody synergistically inhibits tumor growth via promoting robust immune-related antitumor response. This work provides a novel and generalized framework for combining T-cell-impaired targeted therapy and immune checkpoint blockade by using a nanoparticle-based delivery system.
研究领域:
肿瘤学
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药理学
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纳米技术
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免疫疗法
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高影响因子
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
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2026
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IF 3.9
Tianheng Liu,Feng Hong,Renjie Gu,Lixiong Wen
The mixing surface tracking (MS-T) method, a reaction-free approach based on Lagrangian trajectories of fluid elements in CFD simulation, was developed to characterize micromixing under steady-state turbulent flow in T-shaped microreactors. By defining mixing surfaces (MSs) as assemblies of fluid elements of equal mixing time, the evolution of MSs─which visually represented both fluid motion and concentration changes in the mixing process─was quantified using mixing intensity (IM). The micromixing time (tm) was defined as the mixing time required for IM to reach 0.95. The tm values obtained by the MS-T method showed good agreement with those derived from a conventional I–/IO3– reaction system (Villermaux/Dushman method) combined with the incorporation model, confirming the reliability of the MS-T method on micromixing evaluation.
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Frontiers in Immunology
·
2026
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IF 7
Anran Shen,Suyue Zhu,Min Li,Yu Zhao,Yandan Wu,Yue Zhang,Jiejie Zhang,Xuelian Han,Yuan Wang,Guangyu Zhao,Linli Lv,Qi Yin,Taotao Tang
Objective To evaluate the synergistic immunological protection of exosomal T-cell epitope vaccine and antibody-inducing vaccine against SARS-CoV-2 in highly humanized mice. Methods Red blood cell-derived exosomes were loaded with 27 CD8 + T-cell epitope peptides and 19 CD4 + T-cell epitope peptides followed by combined immunization with S1 protein vaccine of SARS-CoV-2 and adjuvant poly I:C in HLA-A2/DR1 double transgenic mice. After immunizations, splenocytes were assessed for epitope-specific T cell responses by intracellular cytokine staining and ELISPOT, and for functional T cell subset analysis through flow cytometry. Meanwhile, serum anti-S1 protein IgG and neutralizing antibodies were quantified via ELISA and BA.5 pseudovirus neutralization assay, respectively. Furthermore, viral challenge was performed after the combined immunization in HLA-A2/DR1/hACE2 triple transgenic mice, followed by viral load quantification, viral protein detection, and H&E staining in lungs. Results The combined immunization i) increased the titers of S1 protein-specific IgG antibodies and neutralizing antibodies as well as Tfh cell frequency as compared to the S1 protein vaccine alone; ii) induced significantly more S1 protein-specific T cells and effector memory CD4 + T cells, and inhibited T cell exhaustion and regulatory T cell differentiation, compared to the exosomal T-cell epitope vaccine alone; iii) achieved the lowest pulmonary viral loads, inflammatory cell infiltration, and histopathological damage after SARS-CoV-2 infection. Conclusion This study, for the first time, demonstrates the synergistic humoral and cellular immune responses and protective efficacy induced by the combined immunization of exosomal T-cell epitope vaccine and antibody-inducing vaccine, and provides preclinical evidence from highly humanized mice for optimizing next-generation SARS-CoV-2 vaccine protocols.
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PubMed
高影响因子
Energy Advances
·
2023
·Vol. 2
·期 12
·2140-2149
·
IF 0
Zhijin Guo,Xuezheng Liang
A novel porous microtube polymer-based solid acid was synthesized via the polymerisation of naphthalene and subsequent sulfonation. The polymer structure could be easily adjusted by altering the amount of formaldehyde dimethyl acetal (FDA), and could be changed to thin slices, microtubes or microspheres. The high surface area of 128.92 m2 g−1 and special microtube structure indicated good mass transfer efficiency and a hydrophobic surface. The abundant naphthalene rings provided sufficient sulfonation sites, which resulted in a high acid density of over 3 mmol g−1. An efficient procedure for biodiesel synthesis and t-butylation of resorcinol were developed using the novel solid acid. The high Brunauer–Emmett–Teller (BET) surface area, special microtube structure and organic framework were the key factors for the high activities, providing great potential in green chemistry.
研究领域:
化学工程
·
催化材料科学
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JOURNAL OF CHEMICAL AND ENGINEERING DATA
·
2021
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IF 2.694
Panxi Wu,Lifeng Zhang,Shujun Zhao,Chenchen Wang,Yongsheng Guo,Wenjun Fang
Biofuel may be an important component of aviation fuel for its significant advantages. In the present work, two biofuel samples, methyl laurate and pinane, are selected for blending research with n-dodecane, a typical model fuel of aviation kerosene. Densities and viscosities for the ternary system pinane (1) + n-dodecane (2) + methyl laurate (3) and corresponding binary systems have been measured at the temperatures from 293.15 to 333.15 K and atmospheric pressure. The excess molar volume (VmE) and viscosity deviation (Δη) of binary systems have been calculated based on experimental data and fitted to the Redlich–Kister equation, while those of the ternary system have been fitted to Nagata–Tamura, Redlich–Kister, Cibulka, and Singh semiempirical equations, respectively. The values of the excess molar volume are all positive for all investigated systems, while the viscosity deviations are all negative. The results are discussed from the perspective of intermolecular force and the structure. Furthermore, the densities and viscosities of both binary and ternary mixtures are satisfactorily correlated using the Jouyban–Acree model.
研究领域:
化学工程
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物理化学
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能源科学
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高影响因子
Interdisciplinary Medicine
·
2026
·
IF 14.5
Ying Zhu,Zhouyu Ning,Yong Deng,Changjing Huang,Hui Li,Yun Sun,Zhiqiang Meng
Resistance to immunotherapy remains a central challenge in advanced hepatocellular carcinoma (HCC). The role of systemic factors, particularly the metabolic fitness of the circulating immune reservoir, in driving this resistance remains poorly understood. This study aimed to identify pre-treatment metabolic signatures in peripheral blood mononuclear cells (PBMCs) that predict therapeutic response and to elucidate the underlying mechanisms of T-cell dysfunction. We performed integrated multi-omics analyses, including proteomics, metabolomics, lipidomics, and phosphoproteomics, on pre-treatment PBMCs from a cohort of HCC patients receiving anti-PD-1 immunotherapy-based combination treatment. Predictive metabolic signatures were identified, and key mechanisms were validated using flow cytometry, confocal microscopy, and molecular analyses. An enhanced lipid metabolic signature in pre-treatment PBMCs was identified as a powerful and independent predictor of poor immunotherapy response and survival. We found that a lipid-enriched plasma milieu in non-responders imposes a metabolic constraint on PBMCs. Specifically, CD36 upregulation correlates with increased lipid uptake and accumulation of glycerophospholipids and sphingolipids, triggering sub-lethal ferroptotic stress (characterized by ferroptosis molecular hallmarks and functional impairment in viable cells without overt cell death) and subsequent T cell exhaustion. This ferroptotic stress state exhibited lipid peroxidation, reduced GPX4 expression, and elevated intracellular iron (Fe 2+ ), and was mechanistically sustained by suppressed AKT survival signaling coupled with hyperactive inflammatory pathways (MAPK, NF-κB). Importantly, this metabolic dysfunction was reversible upon blocking lipid peroxidation. The systemic metabolic fitness of circulating PBMCs is an important determinant of immunotherapy efficacy in HCC that complements tumor-intrinsic factors. Our findings provide a new framework for developing non-invasive predictive biomarkers and demonstrate that PBMC ferroptotic stress is mechanistically reversible, providing a rationale for evaluating systemic metabolic modulation as a strategy to enhance immunotherapy outcomes.
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2026
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IF 9.1
Bin Wen,Fan Chen,Yi Ran,Dongyun Que,Zhihao Zhang,Youwei Zeng,Yuyuan Yao
Molybdenum disulfide (MoS₂) is a promising inorganic co-catalyst for Fenton processes due to its structural durability and avoidance of secondary organic pollution. However, the conventional 2H-phase MoS₂ suffers from intrinsically low conductivity, severely limiting Fe 3+ reduction in the Fenton reaction. Herein, L-cysteine served as a multifunctional precursor during the preparation of MoS₂, promoting a slow-release sulfur supply, carbon‑nitrogen co-doping, and the prevention of restacking. The carbon and nitrogen co-doped molybdenum disulfide (CN-MoS 2 ) architecture was fabricated via a single-step hydrothermal reaction mediated by sodium molybdate, thiourea, and L-cysteine. Remarkably, benefiting from the introduction of L-cysteine, the resulting CN-MoS 2 exhibited a high 1 T-phase content (68%), abundant sulfur vacancies, and a substantially reduced particle size. CN-MoS 2 accomplished near-complete elimination of carbamazepine (CBZ) within merely 3 min, yielding a prominent apparent rate constant of 0.7111 min −1 , which is a 19-fold kinetic acceleration over thiourea-derived MoS 2 . CN-MoS₂ also exhibited high TOC removal, broad-spectrum degradation, and robust CBZ removal under various water conditions. Additionally, electrochemical analyses further verified that CN-MoS₂ reduced charge-transfer resistance and enriched electron density, thereby facilitating rapid electron shuttling to Fe 3+ . Concurrently, quenching experiments and electron paramagnetic resonance (EPR) diagnostics unambiguously validated SO 4 •- , 1 O 2 and O 2 •- as the driving reactive forces. Moreover, a continuous-flow device incorporating a CN-MoS 2 -based membrane exhibited outstanding performance in wastewater purification. This work offers a new strategy for designing high-activity co-catalysts for advanced oxidation processes.
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高影响因子
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