IET Nanobiotechnology
·
2023
·Vol. 17
·期 4
·368-375
·
IF 2.3
Jianmin Chen,Zemin He,Sijin Yu,Xiaozhen Cai,Danhong Zhu,Yanhua Lin
Ellagic acid (EA), which is widely distributed in many foods, has been found to possess inhibitory activity against xanthine oxidase (XO). However, there is ongoing debate about the difference in XO inhibitory activity between EA and allopurinol. Additionally, the inhibitory kinetics and mechanism of EA on XO are still unclear. Herein, the authors systematically studied the inhibitory effects of EA on XO. The authors’ findings showed that EA is a reversible inhibitor with mixed-type inhibition, and its inhibitory activity is weaker than allopurinol. Fluorescence quenching experiments suggested that the generation of EA-XO complex was exothermic and spontaneous. In silico analysis further confirmed that EA entered the XO catalytic centre. Furthermore, the authors verified the anti-hyperuricemia effect of EA in vivo. This study elucidates the inhibition kinetics and mechanism of EA on XO, and lays a theoretical foundation for the further development of drugs and functional foods containing EA for the treatment of hyperuricemia.
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Journal of Materials Chemistry C
·
2023
·Vol. 11
·期 22
·7272-7282
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IF 6.4
Linrong Zhang,Soukaina Khayour,Guozhang Ren,Shunhao He,Junjie Wang,Liuyingzi Yu,Yaxin Song,Chengcheng Zhu,Xing Kang,Yulong Zhang,Zhongyan Gong,Kun Gao,Jin Wang,Huixiang Sheng,Gang Lu,Hai-Dong Yu
Organic electrochemical transistors (OECTs) are promising for highly sensitive chemical and biological detections, because of their signal amplification, low operating voltage, solution compatibility, and high biocompatibility. However, OECTs, particularly the semiconductor layer, still need to be optimized for improving their output performances. Herein, by introducing Nafion, a proton penetrable polymer, into typical semiconductor layers of poly(3,4-ethylenedioxythiophene):polystyrenesulfonate (PEDOT:PSS) and poly(3-hexylthiophene) (P3HT), we realize a significant enhancement (up to 10-fold and 4-fold respectively) in transconductance of OECTs. The enhancements are attributed to the introduction of Nafion domains in the semiconductor channel, which increases the surface area of the semiconductor channel and promotes the ion doping/de-doping of the channel. In addition, the Nafion-including OECTs exhibit a fast response time of 20 milliseconds. Due to the improved transconductance and response time, the obtained OECT demonstrates sensitive detections of the pH value and glucose. This work provides a simple and effective method for improving the performance of OECTs, which will be promising in the sensitive and reliable detection of metabolites.
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FOOD CHEMISTRY
·
2023
·
IF 8.8
Wei Jia,Xin Wang
The potential mechanisms about the health risks of endogenous 3-MCPD remain elusive. Here, we researched the influences of 3-MCPD on the metabolic landscape of digested goat infant formulas via integrative UHPLC-Q-Orbitrap HRMS-MS/MS-based peptidomics and metabolomics (%RSDs ≤ 7.35 %, LOQ 2.99–58.77 μg kg −1 ). Digested goat infant formulas under 3-MCPD-interference caused metabolic perturbation by down-regulating levels of peptides VGINYWLAHK (5.98–0.72 mg kg −1 ) and HLMCLSWQ (3.25–0.72 mg kg −1 ) pertained to health-promoting bioactive components, and accelerated the down-regulation of non-essential amino acids (AAs, l -tyrosine 0.88–0.39 mg kg −1 , glutamic acid 8.83–0.88 μg kg −1 , and d -aspartic acid 2.93–0.43 μg kg −1 ), semi-essential AA ( l -arginine 13.06–8.12 μg kg −1 ) and essential AAs ( l -phenylalanine 0.49–0.05 mg kg −1 ) that provide nutritional value. Peptidomics and metabolomics interactions elucidated that 3-MCPD altered the stability of α-lactalbumin and d -aspartate oxidase in a dose-dependent manner, and affected the flavor perception of goat infant formulas, leading to a decline of nutritional value of goat infant formulas.
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ACS Applied Materials & Interfaces
·
2023
·
IF 9.5
Panpan Hao,Rufeng Zhu,Yang Tao,Wei Jiang,Xue Liu,Yan Tan,Yuedan Wang,Dong Wang
Novel enhancement-mode organic electrochemical transistors (OECTs) have been prepared by poly(3, 4-ethylenedioxythiophene)-poly(styrenesulfonate) de-doped polyethylenimine on the multi-walled carbon nanotube-modified viscose yarn. The fabricated devices exhibit low power consumption with a high transconductance of 6.7 mS, rapid response time < 2 s, and excellent cyclic stability. In addition, the device has washing durability and bending and long-term stability suitable for wearable applications. Biosensors based on enhancement-mode OECTs for the selective detection of adrenaline and uric acid (UA) are developed by using molecularly imprinted polymer (MIP)-functionalized gate electrodes. The detection limits of adrenaline and UA analysis are as low as 1 pM, with the linear ranges of 0.5 pM to 10 μM and 1 pM to 1 mM, respectively. Moreover, the sensor based on enhancement-mode transistors can efficiently amplify the current signals according to the modulation of the gate voltage. The MIP-modified biosensor has high selectivity in the presence of interferents and desirable reproducibility. Additionally, due to the wearable nature of the developed biosensor, this sensing tool has the capability of being integrated with fabrics. Therefore, it has successfully been applied in textiles for the determination of adrenaline and UA in artificial urine samples. The excellent recoveries and rsds are 90.22–109.05% and 3.97–6.94%, respectively. Ultimately, these sensitive, low-power, wearable, and dual-analyte sensors help to develop non-laboratory tools for early disease diagnosis and clinical research.
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Journal of Materials Chemistry A
·
2023
·Vol. 11
·期 23
·12395-12404
·
IF 11.9
He Zhao,Ling Zhang,Tianbo Deng,Chunzhong Li
Wearable sweat sensing technologies have attracted widespread attention for their noninvasive health status monitoring. However, simultaneously achieving high-performance sensing, breathability, and biodegradability is an extreme challenge. Herein, a multifunctional wearable electrochemical biosensor based on a patterned Janus fabric is designed to analyze glucose in sweat continuously. The glucose sensing fiber electrodes have a low-cost but highly efficient glucose-sensing element, cuprous oxide (Cu2O), and the biosensor achieves ultra-high sensitivity (8650.3 μA mM−1 cm−2) and long-term stability (retaining 95.8% of its initial value after 35 days). The biosensor also features outstanding sweat collection efficiency and biodegradation, satisfying the practical application requirements. Meanwhile, a wearable glucose intelligent early warning system is developed based on this multifunctional biosensor integrated with signal processing and wireless communication, combined with data analysis software. In volunteers, this system enabled the real-time monitoring of glucose levels and activated the warning feedback function in an emergency. The integrated multifunctional wearable system demonstrates the ability to intervene remotely for early disease detection and health management.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2023
·
IF 9.9
Yingshuang Meng,Zhibo Qi,Zhonghua Li,Yuchao Niu,Mengyuan Wu,Zhijie Yuan,Gaohong He,Mingyang Yu,Xiaobin Jiang
Herein, a facile bionic research platform with fabricated hydrogel composite membrane (HCM) is constructed to uncover the effects of the main components of coffee’s metabolites on MSUM crystallization. Tailored and biosafety polyethylene glycol diacrylate/ N -isopropyl acrylamide (PEGDA/NIPAM) HCM allows the proper mass transfer of coffee’s metabolites and can well simulate the process of coffee’s metabolites acting in the joint system. With the validations of this platform, it is shown that chlorogenic acid (CGA) can hinder the MSUM crystals formation from 45 h (control group) to 122 h (2 mM CGA), which is the most likely reason that reduces the risk of gout after long-term coffee consumption. Molecular dynamics simulation further indicates that the high interaction energy ( E int ) between CGA and MSUM crystal surface and the high electronegativity of CGA both contribute to the restraint of MSUM crystal formation. In conclusion, the fabricated HCM, as the core functional materials of the research platform, presents the understanding of the interaction between coffee consumption and gout control.
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RAPID COMMUNICATIONS IN MASS SPECTROMETRY
·
2023
·Vol. 37
·期 14
·e9529
·
IF 2
Xin Zhang,Xiaoling Zang,Huanhuan Yang,Peng Jiao,Jie Zhang,Ni Song,Zhihua Lv
Rationale Thymoma is a rare malignant tumor but it is the most common primary tumor of the anterior mediastinum. The current imaging methods for thymoma screening suffer from false positive rate problems, and thymoma pathogenesis remains elusive. Study of thymoma metabolic characteristics could provide clues for improving the diagnosis and understanding the pathogenesis of thymoma. Methods Metabolic profiling of plasma from thymoma and thymic hyperplasia patients was performed using ultrahigh-performance liquid chromatography combined with high-resolution mass spectrometry in both positive and negative ionization modes. After pre- and post-processing, the dataset was divided into three age groups and statistical analysis was performed to select differential metabolites of thymoma. For feature identification, experimental tandem mass spectra were matched to those of databases and available chemical standards, and also manually annotated with plausible chemical structures to ensure high identification confidence. Results A total of 47 differential metabolites were identified in thymoma. Significantly higher levels of histidine, sphinganine 1-phosphate, lactic acid dimer, phenylacetylglutamine, LPC (18:3) and LPC (16:1), and significantly lower levels of phenylalanine, indole-3-propionic acid (IPA), hippuric acid and mesobilirubinogen were associated with thymoma. Tryptophan level in thymoma-associated myasthenia gravis (TAMG) was significantly lower than that of the MG(−) group. IPA and hippuric acid abundances exhibited increasing trends from indolent to aggressive thymoma. Conclusions Our study revealed aberrant aromatic amino acid metabolism and fatty acid oxidation might be associated with thymoma. The identified unique metabolic characteristics of thymoma may provide valuable information for study of the molecular mechanism of thymoma pathogenesis, and improvement of diagnosis and discovery of new therapeutic strategies for thymoma.
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APPLIED SURFACE SCIENCE
·
2023
·
IF 6.7
Chengcheng Qi,Yuhua Luo,Yongping Dong
The study of single atoms in the field of electrochemical sensing is at the early stage. How to boost the catalytic performance of single atoms is still a big challenge. Herein, we prepare Fe-Se dual single-atom sites on N-doped ultrathin carbon carrier (Fe 1 Se 1 /NC) for realizing highly sensitive nonenzymatic detection of H 2 O 2 . Fe 1 Se 1 /NC was synthesized through the pyrolysis strategy, and then was characterized by transmission electron microscopy (TEM), energy dispersive X-ray spectroscopy (EDS) mapping, high-angle annular dark-field scanning transmission electron microscopy (HAADF-STEM), X-ray diffraction (XRD), X-ray photoelectron spectrometer (XPS), and electrochemical techniques, respectively. The results displayed that Fe and Se both existed in an atomically dispersed status. Compared to the single-atom Se or Fe catalyst (Se 1 /NC or Fe 1 /NC), the Fe 1 Se 1 /NC significantly boosted the electrocatalytic activity for H 2 O 2 reduction. The Fe 1 Se 1 /NC modified electrode could be used to detect H 2 O 2 in a wide linear range of 0.02 mM to 13 mM with a high sensitivity of 1508.6 µA·mM −1 ·cm −2 and a low detection limit of 11.5 µM. Moreover, the sensor was successfully employed for detecting H 2 O 2 in disinfectant and urine samples. This work provides a novel design of dual-atom catalysts for excellent electrochemical sensing applications.
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ANALYTICAL CHEMISTRY
·
2023
·
IF 7.4
Hui Dong,Le Zhao,Tao Wang,Yanan Chen,Wanqing Hao,Ziyi Zhang,Yizhao Hao,Cunliang Zhang,Xiuhua Wei,Yintang Zhang,Yanli Zhou,Maotian Xu
Biomarkers detection in blood with high accuracy is crucial for the diagnosis and treatment of many diseases. In this study, the proof-of-concept fabrication of a dual-mode sensor based on a single probe (Re-BChE) using a dual-signaling electrochemical ratiometric strategy and a “turn-on” fluorescent method is presented. The probe Re-BChE was synthesized in a single step and demonstrated dual mode response toward butyrylcholinesterase (BChE), a promising biomarker of Alzheimer’s disease (AD). Due to the specific hydrolysis reaction, the probe Re-BChE demonstrated a turn-on current response for BChE at −0.28 V, followed by a turn-off current response at −0.18 V, while the fluorescence spectrum demonstrated a turn-on response with an emission wavelength of 600 nm. The developed ratiometric electrochemical sensor and fluorescence detection demonstrated high sensitivity with BChE concentrations with a low detection limit of 0.08 μg mL–1 and 0.05 μg mL–1, respectively. Importantly, the dual-mode sensor presents the following advantages: (1) dual-mode readout can correct the impact of systematic or background error, thereby achieving more accurate results; (2) the responses of dual-mode readout originate from two distinct mechanisms and relatively independent signal transduction, in which there is no interference between two signaling routes. Additionally, compared with the reported single-signal electrochemical assays for BChE, both redox potential signals were detected in the absence of biological interference within a negative potential window. Furthermore, it was discovered that the outcomes of direct dual-mode electrochemical and fluorescence quantifications of the level of BChE in serum were in agreement with those obtained from the use of commercially available assay kits for BChE sensing. This method has the potential to serve as a useful point-of-care tool for the early detection of AD.
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Journal of Environmental Sciences
·
2023
·
IF 6.9
Xiang Zhang,Yunpeng Huang,Jikun Wang,Jie Tang,Yang Mei,Nali Zhu,Zhigang Li,Lingxiangyu Li,Yawei Wang
The nanoscale zinc oxide (n-ZnO) was used in food packages due to its superior antibacterial activity, resulting in potential intake of n-ZnO through the digestive system, wherein n-ZnO interacted with saliva. In recent, facet engineering, a technique for controlling the exposed facets, was applied to n-ZnO, whereas risk of n-ZnO with specific exposed facets in saliva was ignored. ZnO nanoflakes (ZnO-0001) and nanoneedles (ZnO-1010) with the primary exposed facets of {0001} and {1010} respectively were prepared in this study, investigating stability and toxicity of ZnO-0001 and ZnO-1010 in synthetic saliva. Both ZnO-0001 and ZnO-1010 partially transformed into amorphous Zn 3 (PO 4 ) 2 within 1 hr in the saliva even containing orgnaic components, forming a ZnO-Zn 3 (PO 4 ) 2 core-shell structure. Nevertheless, ZnO-1010 relative to ZnO-0001 would likely transform into Zn 3 (PO 4 ) 2 , being attributed to superior dissolution of {1010} facet due to its lower vacancy formation energy (1.15 eV) than {0001} facet (3.90 eV). The toxicity of n-ZnO to Caco-2 cells was also dependent on the primary exposed facet; ZnO-0001 caused cell toxicity through oxidative stress, whereas ZnO-1010 resulted in lower cells viability than ZnO-0001 through oxidative stress and membrane damage. Density functional theory calculations illustrated that ·O 2 − was formed and released on {1010} facet, yet O 2 2− instead of ·O 2 − was generated on {0001} facet, leading to low oxidative stress from ZnO-0001. All findings demonstrated that stability and toxicity of n-ZnO were dependent on the primary exposed facet, improving our understanding of health risk of nanomaterials.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2023
·
IF 4.4
Jiling Song,Minghui Chen,Fanlei Meng,Jiahui Chen,Zhanwei Wang,Yong Zhang,Jing Cui,Jing Wang,Dongfang Shi
Xanthine oxidase (XO) is a key enzyme in uric acid production, and its molybdopterin (Mo-Pt) domain is an important catalytic center when xanthine and hypoxanthine are oxidated. It is found that the extract of Inonotus obliquus has an inhibitory effect on XO. In this study, five key chemical compounds were initially identified using liquid chromatography-mass spectrometry (LC-MS), and two compounds, osmundacetone ((3 E )-4-(3,4-dihydroxyphenyl)-3-buten-2-one) and protocatechuic aldehyde (3,4-dihydroxybenzaldehyde), were screened as the XO inhibitors by ultrafiltration technology. Osmundacetone bound XO strongly and competitively inhibited XO with a half-maximal inhibitory concentration of 129.08 ± 1.71 μM, and its inhibition mechanism, was investigated. Osmundacetone and XO via static quenching and spontaneously bound with XO with high affinity, primarily via hydrophobic interactions and hydrogen bonds. Molecular docking studies showed that osmundacetone was inserted into the Mo-Pt center and interacted with hydrophobic residues of Phe911, Gly913, Phe914, Ser1008, Phe1009, Thr1010, Val1011, and Ala1079 of XO. In summary, these findings suggest that provide theoretical basis for the research and development of XO inhibitors from Inonotus obliquus .
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International Journal of Electrochemical Science
·
2023
·
IF 1.5
Bingcheng Su,Yan Chen,Xiangli Yang,Juan Han,Hongyu Jia,Pei Jing,Yu Wang
Detection of vitamin C (VC) is necessary because of its wide use in chemical, biological, and pharmaceutical engineering. Here, we describe a sensing system for the determination of VC. Instead of a bare glassy carbon electrode (GCE), a prepared multi-walled carbon nanotube-polycation-GCE was used to detect VC in samples with higher sensitivity, better repeatability, and with a lower detection limit. Under the optimal conditions, the obtained sensor presented a linear response to VC in the range of 1–100 μM with a detection limit of 500 nM. The proposed electrode also successfully detected VC concentrations in real samples.
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