RSC Advances
·
2025
·Vol. 15
·期 14
·10862-10872
·
IF 4.6
Muneera Alrasheedi,Salah M. El-Bahy,Refat El-Sayed,Khaled F. Debbabi,Alaa S. Amin
Although modern reported methods, such as AAS, ICP-AES, ICP-MS, have good sensitivity, the high cost of equipment, the need for sophisticated instruments, separation and preconcentration steps and experienced technicians along with lack of precise methods make them cumbersome. Solid phase extraction (SPE) emerges as an attractive technique that reduces solvent consumption, minimizes exposure, shortens extraction time, and lowers disposal costs. Herein, a pioneering methodology for the quantification of minute amounts of silver is introduced, using 2-nitro-6-(thiazol-2-yl-diazenyl)phenol (NTDP) as a complexing agent and Triton X-100 as a nonionic surfactant within a ternary surfactant system at a pH of 5.3. This novel extraction strategy demonstrated selective preconcentration. The enriched solution was subjected to spectrophotometric analysis for the quantification of the analyte. After refining extraction and complexation parameters, a remarkable 250-fold increase in the enrichment factor was attained, highlighting a sensitivity boost of 509 times compared with traditional extraction approaches relying solely on nonionic surfactants. The key parameters of molar absorptivity and Sandell sensitivity were determined to be 6.04 × 106 L mol−1 cm−1 and 0.0018 ng cm−2, respectively. The calibration plot was observed from 5.0–175 ng mL−1, whereas Ringbom optimum concentrations ranged from 15–160 ng mL−1. The detection and quantification limits were 1.63 and 4.95 ng mL−1, respectively. The relative standard deviation (RSD) of the complex was 2.27. The suggested method was efficiently utilized for assessing the Ag+ concentration in real samples, producing acceptable outcomes.
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JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
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2013
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IF 2.487
Lingchen Yang,Zhiyong Zhao,Aibo Wu,Yifeng Deng,Zhenlei Zhou,Jianpeng Zhang,Jiafa Hou
A stable and sensitive method has been developed for use in food and livestock product safety for the detection of mycotoxins. This newly developed method allows for the determination of T-2 toxin, HT-2 toxin and diacetoxyscirpenol (DAS) in heart, liver, spleen, lung, kidney, Glandular stomach, muscular stomach, small intestine, muscle, bone and brain samples from broilers using liquid chromatography coupled to tandem mass spectrometry (LC–MS/MS). The samples were initially extracted with ethyl acetate before being filtered through a 0.22 μm nylon syringe filter and subjected to chromatographic separation on a reversed-phase C18 (50 × 2.1 mm, 3 μm) column. A mobile phase composed of 0.1% acetic acid and 10 mM ammonium acetate in methanol and water was used in an assay of the levels of T-2 toxin, HT-2 toxin and DAS. For the analysis of the target compounds, the mass spectrometer was operated under positive electrospray ionization conditions in the selected reaction monitoring mode. The limit of detection was in the range of 0.02–0.05 ng/g, whereas the limit of quantification was in the range of 0.08–0.15 ng/g. The extraction recoveries of spiked samples from the high, intermediate and low levels ranged from 58.5% to 110.5%, and the relative standard deviation (RSD (%)) values were less than 17.0%. The results of inter- and intra-day precision (RSD (%)) were within 14.7%. The results revealed that the present method could be successfully applied to the analysis of T-2 toxin, HT-2 toxin and DAS in the real samples
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BIOMACROMOLECULES
·
2025
·
IF 5.4
Fei Jiang,Qing Huang,Xianqing Lv,Zhiming Dong,Zhuotong Wu,Lu Chen,Jianguo Li,Lihui Chen,Li Shuai,Chengke Zhao
Selective oxidation of polyol-containing cellulose is significant for the development of cellulose-derived functional materials. While conventional oxidation methods readily introduce aldehyde groups at the C2/C3 positions (accompanied by ring-opening of anhydroglucose units) or carboxyl groups at the C6 positions in cellulose, selective oxidation to generate aldehyde functionalities specifically at the C6 position remains challenging. Herein, we demonstrate that 2-Iodoxybenzoic acid (IBX) acts as an efficient and selective oxidant for converting the primary alcohol at the C6 position of anhydroglucose units to aldehyde groups under mild conditions. Monosaccharide model studies confirmed C6-specific oxidation. This method was successfully applied for the surface modification of microcrystalline cellulose (MCC, predominantly Iβ allomorph) and bacterial cellulose (BC, mainly Iα allomorph), achieving aldehyde contents of 0.96 and 0.93 mmol g-1, respectively, with 0.6 and 0.5 equiv of IBX per anhydroglucose unit. Crystallinity analysis showed that oxidation with IBX leads to a significant decrease of crystallinity but has a negligible effect on the cellulose allomorph. This study presents a novel approach for the preparation of C6-aldehyde cellulose, enabling precise, selective modification and advancing functional material development.
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VIROLOGY
·
2025
·
IF 2.4
Yajie Chen,Guangfu Yu,Shan Gao,Leiliang Zhang
Herpes simplex virus type 2 (HSV-2) is a common sexually transmitted pathogen known for causing genital herpes. Glycoprotein B (gB) of HSV-2 plays a crucial role in viral entry and infection. However, its post-translational modifications are not well understood. This study investigated the palmitoylation of HSV-2 gB, identifying cysteine residue 8 (C8) as a critical palmitoylation site. Using an acyl-biotin exchange assay, we confirmed that gB was indeed palmitoylated, and treatment with 2-bromopalmitate significantly reduced this modification. Further analysis revealed that ZDHHC14 interacts with gB and facilitated its palmitoylation. We also identified APT2 as a negative regulator of gB palmitoylation. Importantly, palmitoylation enhanced gB's localization to the plasma membrane, whereas the C8S mutation substantially impaired this localization. Functionally, palmitoylation of gB enhanced the infective efficiency of HSV-2 pseudotyped particles (pp) and live virus, while both palmitoyltransferase and APT2 inhibition affected the entry efficiency of HSV-2pp. Our findings demonstrate that the palmitoylation of gB is crucial for its localization to the membrane and for facilitating efficient HSV-2 infection, highlighting palmitoylation as a promising target for antiviral interventions and providing new insights into the pathogenesis of HSV-2.
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CATALYSIS COMMUNICATIONS
·
2023
·
IF 3.7
Hao Li,Huimin Liu,Chiliu Cai,Haiyong Wang,Youwang Huang,Song Li,Bin Yang,Chenguang Wang,Yuhe Liao,Longlong Ma
Nowadays, 2-methylfuran is urgently needed especially in pharmaceutical manufacture. However, the direct synthesis of 2-methylfuran via the efficient dehydration and hydrogenation of xylose usually requires a multistep reaction. Herein, a route of tandem conversion xylose to 2-methylfuran was developed. The xylose was firstly converted into furfural by Hβ zeolite, followed by the catalysis of furfural to 2-methylfuran over 0.5NiCu/C. Compared with two-step reaction, cascade strategy applied xylose conversion improved the 2-methylfuran yield rate by 41.5%. In general, this work expanded the application of tandem method for the enhancement of xylose effective conversion and provided a new biomass utilization technology.
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FITOTERAPIA
·
2022
·
IF 3.204
Chao Wang,Quan-Hao Wei,Zhen-Qiang Xin,Lin-Lin Tian,Jun-Sheng Zhang,Hua Zhang
Chemical fractionation of the EtOH extract of the roots of a traditional Chinese herb, Morinda officinalis , afforded an array of methyl 2-naphthoate derivatives ( 1 – 9 ) including four pairs of enantiomers ( 1 – 4 ), two pimarane diterpenes and two ursane triterpenoids . Among them, eight compounds ( 1a / 1b - 3a / 3b , 11 and 13 ) were reported in the current work for the first time. The structures of the new compounds, including their absolute configurations, were defined by spectroscopic analyses in combination with quantum chemical electronic circular dichroism (ECD) and gauge-independent atomic orbital (GIAO) NMR calculations. All the isolates were evaluated for their inhibitory effect on nitric oxide (NO) production induced by lipopolysaccharide (LPS) in murine RAW264.7 macrophage cells, and the enantiomers 1a and 3b exhibited moderate activity with IC 50 values of 41.9 and 26.2 μM. Meanwhile, compound 3b also dose-dependently inhibited the secretion of two pro-inflammatory cytokines TNF-α and IL-6 in the same cell model.
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JOURNAL OF MOLECULAR LIQUIDS
·
2022
·
IF 6.633
Zhiyin Yao,Na Du,Nan Chen,Jianqiang Liu,Wanguo Hou
Proto-single chain amphiphiles (P-SCAs) vesicles are considered to be a plausible model of the protocell on early Earth due to their primordial relevancy and membrane structure. In this work, we selected P-SCAs sodium 2-ketooctanate (SKO) and proto-nucleobases (PNBs, including cytosine, uracil, adenine, and guanine) as the building constituents of the vesicles. The PNB molecules incorporate into SKO bilayers to form PNB@SKO vesicles with a binding rate of 7% − 12%. The interactions between them increase the solubility of PNB in water obviously while decreasing the critical vesicle concentration of SKO. The morphology, size, and membrane thickness of SKO PRB@SKO vesicles are not affected by the PNB combination significantly. A binding mechanism is proposed based on the results of Fourier transform infrared and small angle X-ray scattering. The rigid planar PNB molecules orientate via hydrogen bonding in the tilted bilayers of SKO, without excessive disruption to the membrane structure. Both ribozyme-catalyzed and non-enzymatic RNA copying reactions require high concentrations of divalent ions, fortunately, the salt resistance of these PNB@SKO vesicles is surprisingly remarkable, one or two orders of magnitude greater than the other typical P-SCAs vesicles systems. We expect that this primitively relevant vesicle system can exert its potential advantages in the proto-membrane mimicry of the origin of life.
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CELL RESEARCH
·
2021
·Vol. 31
·期 10
·1047-1060
·
IF 25.617
Hu Wei,Zhang Yong,Fei Panyu,Zhang Tongtong,Yao Danmei,Gao Yufei,Liu Jia,Chen Hui,Lu Qiao,Mudianto Tenny,Zhang Xinrui,Xiao Chuxuan,Ye Yang,Sun Qiming,Zhang Jing,Xie Qi,Wang Pei-Hui,Wang Jun,Li Zhenhai,Lou Jizhong,Chen Wei
The outbreak of SARS-CoV-2 (SARS2) has caused a global COVID-19 pandemic. The spike protein of SARS2 (SARS2-S) recognizes host receptors, including ACE2, to initiate viral entry in a complex biomechanical environment. Here, we reveal that tensile force, generated by bending of the host cell membrane, strengthens spike recognition of ACE2 and accelerates the detachment of spike’s S1 subunit from the S2 subunit to rapidly prime the viral fusion machinery. Mechanistically, such mechano-activation is fulfilled by force-induced opening and rotation of spike’s receptor-binding domain to prolong the bond lifetime of spike/ACE2 binding, up to 4 times longer than that of SARS-S binding with ACE2 under 10 pN force application, and subsequently by force-accelerated S1/S2 detachment which is up to ~10 3 times faster than that in the no-force condition. Interestingly, the SARS2-S D614G mutant, a more infectious variant, shows 3-time stronger force-dependent ACE2 binding and 35-time faster force-induced S1/S2 detachment. We also reveal that an anti-S1/S2 non-RBD-blocking antibody that was derived from convalescent COVID-19 patients with potent neutralizing capability can reduce S1/S2 detachment by 3 × 10 6 times under force. Our study sheds light on the mechano-chemistry of spike activation and on developing a non-RBD-blocking but S1/S2-locking therapeutic strategy to prevent SARS2 invasion.
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ACS Sensors
·
2021
·
IF 7.711
Yongzhong Jiang,Menglu Hu,An-An Liu,Yi Lin,Linlin Liu,Bo Yu,Xiaoming Zhou,Dai-Wen Pang
The outbreak of COVID-19 caused a worldwide public health crisis. Large-scale population screening is an effective means to control the spread of COVID-19. Reverse transcription–polymerase chain reaction (RT-qPCR) and serology assays are the most available techniques for SARS-CoV-2 detection; however, they suffer from either less sensitivity and accuracy or low instrument accessibility for screening. To balance the sensitivity, specificity, and test availability, here, we developed enhanced colorimetry, which is termed as a magnetic pull-down-assisted colorimetric method based on the CRISPR/Cas12a system (M-CDC), for SARS-CoV-2 detection. By this method, SARS-CoV-2 RNA from synthetic sequences and cultured viruses can be detected by the naked eye based on gold nanoparticle (AuNP) probes, with a detection limit of 50 RNA copies per reaction. With CRISPR/Cas12a-assisted detection, SARS-CoV-2 can be specifically distinguished from other closely related viruses. M-CDC was further used to analyze 41 clinical samples, whose performance was 95.12%, consistent with that of an approved Clinical RT-qPCR Diagnosis kit. The developed M-CDC method is not dependent on sophisticated instruments, which makes it potentially valuable to be applied for SARS-CoV-2 screening under poor conditions.
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EUROPEAN JOURNAL OF NEUROSCIENCE
·
2023
·Vol. 58
·期 10
·4123-4136
·
IF 3.4
Hong Ni,Zhongzhao Guo,Yue Wu,Jie Wang,Yang Yang,Zilu Zhu,Deheng Wang
It is generally accepted that Cyclooxygenase-2 (COX-2) is activated to cause inflammation. However, COX-2 is also constitutively expressed at the postsynaptic dendrites and excitatory terminals of the cortical and spinal cord neurons. Although some evidence suggests that COX-2 release during neuronal signalling may be pivotal for regulating the function of memory, the significance of constitutively expressed COX-2 in neuron is still unclear. This research aims to discover the role of COX-2 in memory beyond neuroinflammation and to determine whether the inhibition of COX-2 can cause cognitive dysfunction by influencing dendritic plasticity and its underlying mechanism. We found COX-2 gene knockout (KO) could significantly impact the learning and memory ability, cause neuronal structure disorder and influence gamma oscillations. These might be mediated by the inhibition of prostaglandin (PG) E2/cAMP pathway and phosphorylated protein kinase A (p-PKA)-phosphorylated cAMP response element binding protein (p-CREB)-brain derived neurotrophic factor (BDNF) axis. It suggested COX-2 might play a critical role in learning, regulating neuronal structure and gamma oscillations in the hippocampus CA1 by regulating COX-2/BDNF signalling pathway.
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International Journal of Electrochemical Science
·
2023
·
IF 1.5
Jia Du,Xu Peng,Yuting Xu,Li Fu,Di Cao,Qinghua Liu
Odorant substances have been detected in the drinking water of several countries and regions. Although the specific harm of these substances to the human body is not yet clear, they can lead to the decline of drinking-water sensory indicators. In addition, odorants also affect the development of the waterdependent beverage industry and aquaculture industry. Dimethyl isoborneol (2-MIB) is the most common odorant substance that causes soil mildew in drinking water. In this work, for the first time, we first report the electrochemical determination of 2-MIB based on an Au electrode. The electrochemical oxidation of 2-MIB can be achieved due to the excellent conductivity and electrocatalytic property of the Au electrode. The proposed electrochemical sensing method is simple, quick and reliable for 2-MIB determination.
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Cell Death & Disease
·
2023
·Vol. 14
·期 4
·1-10
·
IF 9
Xia Kangkai,Li Shujing,Yang Yuxi,Shi Xiaoxia,Zhao Binggong,Lv Linlin,Xin Zhiqiang,Kang Jie,Ren Ping,Wu Huijian
Breast cancer is the most commonly diagnosed cancer, and its global impact is increasing. Its onset and progression are influenced by multiple cues, one of which is the disruption of the internal circadian clock. Cryptochrome 2 (Cry2) genetic dysregulation may lead to the development of some diseases and even tumors. In addition, post-translational modifications can alter the Cry2 function. Here, we aimed to elucidate the post-translational regulations of Cry2 and its role in breast cancer pathogenesis. We identified p300-drived acetylation as a novel Cry2 post-translational modification, which histone deacetylase 6 (HDAC6) could reverse. Furthermore, we found that Cry2 inhibits breast cancer proliferation, but its acetylation impairs this effect. Finally, bioinformatics analysis revealed that genes repressed by Cry2 in breast cancer were mainly enriched in the NF-κB pathway, and acetylation reversed this repression. Collectively, these results indicate a novel Cry2 regulation mechanism and provide a rationale for its role in breast tumorigenesis.
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