PROCESS BIOCHEMISTRY
·
2017
·
IF 2.497
Shu-Ping Zou,Ji-Wei Huang,Ya-Ping Xue,Yu-Guo Zheng
1-Cyanocyclohexaneacetic acid (1-CHAA) is an important intermediate in the preparation of gabapentin. Regioselective biotransformation of 1-cyanocyclohexaneacetonitile (1-CHAN) into 1-CHAA, mediated by the immobilization of recombinant microbial nitrilase, is a novel and promising strategy for its production. In this study, a recombinant E. coli expressing Acidovorax facilis nitrilase was flocculated with polyethylenemine, followed by cross-linking with glutaraldehyde to obtain cross-linked cell aggregates (CLCAs). Under the optimum preparation conditions, the activity recovery of CLCAs reached 68%. Characterization of the CLCAs showed that the optimum pH was not affected by cross-linking, but cross-linking resulted in slightly different optimum temperature. The half-life of CLCAs at 45 °C was 266.5 h, which is 11.6-times higher than that of the free cells. Preparative-scale biotransformation of 1-CHAN to 1-CHAA at high substrate loading (up to 1.0 M) was performed using CLCAs as the biocatalyst in a 2L stirred reactor, resulting in an conversion of 91%, with a productivity of 244.5 g L −1 d −1 . The CLCAs also exhibited a high operational stability and even after 30 cycles of reaction, it retained >80% of the initial conversion.
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高影响因子
POLYMER
·
2024
·
IF 4.1
Rui Hong,Ri-Tong Lan,Yue Ren,Lu Xu,Yun-Xiang Xu,Nouman Ali Shah,Rizwan M. Gul,Shishu Huang,Lingli Li,Jia-Zhuang Xu,Zhong-Ming Li,Ka Li
Oxidation resistance is strongly necessary for the use of crosslinked ultrahigh molecular weight polyethylene (UHMWPE) in artificial joints. The hybrid antioxidant strategy emerged as a promising alternative to the single rival that relies on vitamin E (VE). The goal of this study was to investigate the universality of the antioxidant synergy between VE and sugar alcohol (SA). Three types of SA, including D-sorbitol, maltitol and erythritol, were employed to cooperate with VE. Compared to the use of VE alone, these hybrid antioxidants generally enabled the corresponding UHMWPE composites with prolonged oxidation induction time (OIT) and increased crosslink density. The correlation between OIT and crosslink density of VE/SA-stabilized UHMWPE composites showed low attenuation. The applicability of the hybrid antioxidants to protect UHMWPE against oxidation was further demonstrated using the in vitro clinically relevant accelerated aging. Among the used SA, D-sorbitol exhibited the strongest ability to enhance the synergetic efficiency. The possible mechanism was revealed from the following aspects: the number of secondary hydroxyls per molecular mass and the molecular polarity determined via polarity parameter calculation. These findings provide significant insights for fabricating UHMWPE bearing materials with superior comprehensive performance and extended service longevity.
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高影响因子
Surfaces and Interfaces
·
2024
·
IF 5.7
Ping Lu,Zixing Gu,Zihan Zhang,Huaneng Su,Qiang Ma,Chunsheng Li,Lei Wei,Qian Xu
The all-in-one E-fueled solar flow battery system that can be recharged with liquid fuel is a new type of power system. It can continuously convert solar energy into liquid fuel without relying on external power supply equipments. However, the interface between the photoanode and the electrolyte often exhibits unsteady behavior, resulting in poor performance of the system. In this work, the {201} facets are selected and the impact of two doping treatment strategies for TiO 2 {201} photoanode on the performance of the E-fueled solar flow battery system is systematically studied through the I-t curve, linear sweep voltammetry, Mott-Schottky plot. The results show that the un-biased photocharging current of bulk-doped TiO 2 can reach 2.7 mA·cm −2 in the battery system test, only relying on the self-built electric field at the interface, and the energy conversion efficiency approaches 2.67 %. In addition, the performance is analyzed by the combination of transmission electron microscopy, X-ray diffraction, X-ray photoelectron spectroscopy and other characterizations, with the aim of distinguishing the effects of bulk- and surface-doped Mn and accurately revealing the mechanism of their influences from the outside to the inside. Finally, the optimization rules of the un-biased light charging mode of the system are explored.
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高影响因子
Analytical Methods
·
2024
·Vol. 16
·期 28
·4865-4872
·
IF 2.7
Yujie Wen,Cong Tang,Qing Shen,Shuqing Dong,Yaya Wang,Yunchun Li,Shijun Shao
Developing an effective and convenient nitrite detection method is indispensable in food safety, environmental monitoring, clinical diagnosis of diseases, and many other areas. Herein, a dicyanoisophorone derivative, TMN-NH2 with large Stokes shift and near-infrared (NIR) emission, was proposed as a ratiometric fluorescence and colorimetric dual-mode probe for the rapid determination of NO2− in acidic media, showing excellent selectivity and high sensitivity. The sensing mechanism is based on the diazotization of TMN-NH2 with NO2− and subsequent diazonium salt hydrolysis to form a hydroxyl-substituted product (TMN-OH). Under the optimized conditions of reaction and detection, a new quantitative analysis method based on TMN-NH2 was established for NO2− detection, exhibiting good linear relationships to NO2− in the range of 0.5 to 15 μM with practical detection limits of 26.6 nM and 17.6 nM for the colorimetric and fluorescent readout, respectively. The quantitative detection of NO2− in real samples demonstrated satisfactory recoveries and repeatability. Moreover, TMN-NH2 was successfully applied for monitoring NO2− in Escherichia coli by confocal fluorescence imaging.
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PubMed
高影响因子
International Journal of Nanomedicine
·
2024
·
IF 6.6
Yaxu Feng,Qing Sun,Pei Liu,Wei Fan,Bing Fan
Background Enterococcus faecalis (E. faecalis) is one of the main pathogens responsible for refractory root canal infections in the teeth and shows resistance against various antibacterial managements. Effective control of E. faecalis infection is a prerequisite for successful treatment of refractory apical periodontitis. This study aimed to analyze the antibacterial activity and mechanisms of Au@Ag nanoparticles (NPs) combined with photothermal therapy (PTT) against the original and Ag+-resistant E. faecalis.Methods Au@AgNPs with optimal shell thicknesses were synthesized and characterized. The antibacterial activity of Au@AgNPs with PTT against the original or Ag+-resistant E. faecalis was evaluated, and the antibiofilm activity was tested on E. faecalis biofilm on the dentin of teeth. The potential antibacterial mechanisms of Au@AgNPs combined with PTT against E. faecalis have also been studied. Moreover, its influence on dentin microhardness and cytotoxicity was assessed.Results This study revealed that Au@AgNPs combined with PTT showed enhanced antibacterial and antibiofilm effects, no negative effects on dentin microhardness, and low cytotoxicity toward human periodontal ligament cells (hPDLCs). Moreover, Au@AgNPs combined with PTT effectively inhibited the growth of Ag+-resistant E. faecalis. Its antibacterial effects may be exerted through the release of silver ions (Ag+), destruction of the cell membrane, production of reactive oxygen species (ROS) and inhibition of adenosine triphosphate (ATP) production. Hyperthermia generated by Au@AgNPs with PTT reduced membrane fluidity and enhanced Ag+ sensitivity by downregulating fabF expression. The upregulated expression of heat shock genes demonstrated that the Ag+ released from Au@AgNPs compromised the heat adaptation of E. faecalis.Conclusion PTT significantly enhanced Ag+ sensitivity of the original and Ag+-resistant E. faecalis. Au@AgNPs combined with PTT may have the potential to be developed as a new antibacterial agent to control E. faecalis infections in teeth.
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PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2024
·
IF 7
Xiaoli Tan,Xiaobing Jiang,Okwong Oketch Reymick,Chen Zhu,Nengguo Tao
Stem-end rot caused by Neofusicoccum parvum is among the most detrimental diseases affecting postharvest mangoes. The present investigation utilized ( E )-2-octenal to manage N. parvum infections, elucidating its mechanism of action. The findings revealed that ( E )-2-octenal exhibited outstanding antimicrobial potency against N. parvum in vitro within the concentration range of 0.4–1.6 µL/mL. Additionally, ( E )-2-octenal significantly compromised the cell membrane integrity and mitochondrial energy metabolism of N. parvum , evidenced by dramatically increased intracellular material leakage and ROS levels, along with reduced mitochondrial membrane potential, ATP, and energy charge. Further experiments showed noteworthy effects on the tricarboxylic acid cycle (TCA) cycle and the key enzyme activities of succinate dehydrogenase (SDH) and malate dehydrogenase (MDH). Molecular docking revealed hydrogen bonding between ( E )-2-octenal’s aldehyde group and SDH (Trp-307) and MDH (Gly-101), indicating direct targeting of these enzymes for inhibition. To enhance the practical application of ( E )-2-octenal, we developed an aerogel-loaded ( E )-2-octenal material (ALO) that exhibited superior antimicrobial efficacy in vitro . In vivo , ALO effectively controlled mango stem-end rot, with optimal efficacy at 20 µL/L. This concentration also delayed the natural disease of mango without degrading fruit quality. According to these findings, ( E )-2-octenal is a promising preservative against postharvest mango infections, potentially by impeding cellular energy metabolism through direct interaction with SDH and MDH within the TCA pathway, culminating in mitochondrial dysfunction and cell membrane damage.
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PubMed
高影响因子
Nanomaterials
·
2024
·Vol. 14
·期 23
·1958
·
IF 4.4
Yan Mo,Xiaoping Zhang,Ke Zou,Wen Xing,Xiayang Hou,Yu Zeng,Yugang Cai,Ruixiang Xu,Hongwen Zhang,Weiping Cai
Etomidate (ET), a medical anesthetic, is increasingly being incorporated into e-liquids for consumption and abuse as a new psychoactive substance (NPS), leading to significant social issues. In this work, large-area Au micro- and nano-structured ordered arrays were engineered as surface-enhanced Raman spectroscopy (SERS) substrates for fast detection and precise identification of ET and its metabolites. This ordered array, characterized by abundant electromagnetic enhancement hotspots and structural uniformity, imparts unique properties to the SERS substrate, including ultra-sensitivity, spectral signal reproducibility, and precise quantitative capabilities. Furthermore, it effectively mitigates interference from the complex matrix of e-liquids, facilitating the rapid detection of trace amounts of ET molecules. This SERS rapid detection technology can act as a preliminary screening method for gold-standard spectroscopic analysis, facilitating the on-site rapid screening of suspicious samples and thereby enabling efficient detection and precise verification.
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DOI
PubMed
高影响因子
PLoS One
·
2025
·Vol. 20
·期 5
·e0324164
·
IF 2.6
Bingbing Cao,Jiali Zhou,Bo Xia,Xiaoqing Li,Rui Wang,Yiqiao Xu,Chunqi Li
Choline has been proven to be effective in maintaining liver function. However, the effect of choline, in combination with other nutrients, on the improvement of non-alcoholic fatty liver disease (NAFLD) remains unclear. This study aimed to investigate the potential effect of the nutraceutical complex containing choline bitartrate, zinc citrate, and dl-α-Tocopheryl acetate on NAFLD in the zebrafish model. The NAFLD model was induced in zebrafish by administering thioacetamide. Experimental groups were established, including a normal control group, the model control group, the positive control group, the nutraceutical complex intervention group, and the choline bitartrate alone intervention group. The intervention was administered to the zebrafish in a water-soluble form, while the positive control group received polyene phosphatidylcholine at a concentration of 50.0 μg/mL. Notably, the protective effect of the nutraceutical complex against NAFLD is more pronounced than that observed with choline bitartrate supplementation alone. The results of transcriptomics and quantitative real-time PCR showed that the potential mechanisms underlying the effects of the nutraceutical complex might involve the upregulation of acacia, acsl1a , fbp2 gene expression, and the downregulation of tbc1d1 gene expression. These results were further validated by western blotting and overexpression experiments. Our findings indicated that choline bitartrate, zinc citrate, and dl-α-Tocopheryl acetate can help improve NAFLD. The results of this study provide evidence for the application of the nutraceutical complex in the improvement of NAFLD.
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高影响因子
Journal of Materiomics
·
2021
·
IF 6.425
Yang Yin,Jing-Ru Yu,Yu-Cheng Tang,Ai-Zhen Song,Huan Liu,Dong Yang,Jing-Feng Li,Lei Zhao,Bo-Ping Zhang
NaNbO 3 -based ceramics usually show ferroelectric-like P-E loops at room temperature due to the irreversible transformation of the antiferroelectric orthorhombic phase to ferroelectric orthorhombic phase, which is not conducive to energy storage applications. Our previous work found that incorporating CaHfO 3 into NaNbO 3 can stabilize its antiferroelectric phase by reducing the tolerance factor ( t ), as indicated by the appearance of characteristic double P-E loops. Furthermore, a small amount of MnO 2 addition effectively regulate the phase structure and tolerance factor of 0.94NaNbO 3 -0.06CaHfO 3 (0.94NN-0.06CH), which can further improve the stability of antiferroelectricity. The XRD and XPS results reveal that the Mn ions preferentially replace A-sites and then B-sites as increasing MnO 2 . The antiferroelectric orthorhombic phase first increases and then decreases, while the t shows the reversed trend, thus an enhanced antiferroelectricity and the energy storage density W rec of 1.69 J/cm 3 at 240 kV/cm are obtained for 0.94NN-0.06CH-0.5%MnO 2 (in mass fraction). With the increase of Mn content to 1.0 % from 0.5 %, the efficiency increases to 81 % from 45 %, although the energy storage density decreases to 1.31 J/cm 3 due to both increased tolerance factor and non-polar phase.
研究领域:
材料科学
·
陶瓷
·
储能
·
电陶瓷
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DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2020
·
IF 6.785
Xuan Sun,Yu Wang,Lu Zhang,Sha Liu,Man Zhang,Jiang Wang,Baoan Ning,Yuan Peng,Jing He,Yonggang Hu,Zhixian Gao
Escherichia coli O157:H7 has been reported as an important pathogenic bacteria causing serious infection and economic loss. However, detection of Escherichia coli O157:H7 needs improvement, given its current complexity and sensitivity. Herein, we attempt to build a fluorescence sensing method to detect Escherichia coli O157:H7 with easy operation and high efficiency. The target virulence gene sequences are recognized and cleaved by the CRISPR-Cas9 system, and trigger strand displacement amplification and rolling circle amplification. After amplification reactions, massive products can hybridize with the probes, the fluorescence of which are quenched based on a metal–organic framework platform, leading to the fluorescence recovery at typical excitation/emission wavelengths of 480/518 nm. This method exhibits high sensitivity with the detection limit at 4.0 × 101 CFU mL–1 and a wide range from 1.3 × 102 CFU mL–1 to 6.5 × 104 CFU mL–1. Meanwhile, this assay also shows significant specificity and applies to practical samples with high accuracy. Therefore, our method would have great potential application in bacterial detection, food safety monitoring, or clinical diagnostics.
研究领域:
微生物学
·
生物技术
·
食品科学
·
分子生物学
·
分析化学
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PubMed
高影响因子
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
·
2026
·
IF 3.5
Margarita A. Arbuzova,Olga Yu Selyutina,Nikolay E. Polyakov
Cholesterol drives E-protein depth redistribution via a matrix-mediated helix tilt adjustment. • Escin-induced peptide localization shifts strictly require the presence of charged DMPS lipids. • Escin is hypothesized to modulate the viroporin conformation via localized helix kinking rather than matrix disruption. • Escin is able to extract cholesterol from lipid membrane. β-Escin is a well-known saponin derived from the horse chestnut tree. Saponins, including escin, exhibit a wide range of biological activities, among which membrane-modifying activity is particularly notable. The latter is key to understanding the molecular mechanisms underlying escin's biological effects, including its antiviral properties. For example, altering membrane properties can strongly influence the life cycle of enveloped viruses such as SARS-CoV-2 by modulating the activity of its envelope protein (E protein). In the present paper, we studied the localization of both escin and the E protein transmembrane domain (ETM) in model membranes with different lipid compositions. We demonstrated that escin can alter ETM localization in membranes containing the negatively charged lipid DMPS, and that escin is also capable of extracting cholesterol from model lipid membranes. Download: Download high-res image (423KB) Download: Download full-size image
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高影响因子
Journal of Mechanical Science and Technology
·
2026
·
IF 2.2
Khalil Muhammad Kaleem Ullah,Gul Rizwan M.,Shah Nouman Ali,Jan Syed Rooh Ullah,Ahmad Adnan,Kamran M. Ali,Xu Jia-Zhuang,Li Zhong-Ming
Wear and oxidation resistance are among the primary requirements for successful and sustainable total joint replacements (TJRs). Both of these factors are major causes of TJRs failure. To address these issues and enhance the service life of TJRs, the effects of vitamin E (VE) and vitamin E-D-sorbitol (VE-DS), which are antioxidants (AOs), on stabilizing ultrahigh-molecular-weight polyethylene (UHMWPE) are presented. These antioxidants were blended with control-UHMWPE at a concentration of 0.3 % by weight and consolidated by compression molding. Wear occurs on the surface, and restricting the crosslinking to a layer on the surface is desirable, as bulk crosslinking reduces the toughness and fatigue strength of the implant. In this study, surface chemical crosslinking of control-UHMWPE, VE-UHMWPE and VE-DS-UHMWPE with dicumyl peroxide (DCP) is presented. The crosslinked density, crystallinity, wear volume, mechanical performance, biocompatibility and oxidation stability assessed by oxidation induction time (OIT) of the UHMWPE blends were characterized through different techniques. The control-UHMWPE blend showed the highest wear resistance but the lowest OIT, while the VE-DS-UHMWPE blend displayed highest crystallinity, best mechanical performance and the highest OIT, exhibiting the synergistic effect of vitamin E and D-sorbitol on the oxidation resistance of UHMWPE.
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高影响因子
危险品化学品经营许可证（带存储）