JOURNAL OF ENVIRONMENTAL MANAGEMENT
·
2024
·
IF 8
Zhineng Wu,Hanyu Niu,Jiao Wang,Runnan Guo,Zixuan Yang,Gaolei Liang,Xiaodong Ma
Trichloroethylene (TCE) is a prevalent organic pollutant found in soil. The oxide passivation layer on the surface of micron iron powder inhibits the release of its reducing components, leading to ineffective reduction and purification of TCE in soil. To enhance TCE degradation, a slow-release reduction material " Escherichia sp. F1-micron iron powder" was developed. A novel iron-reducing bacterium, Escherichia sp. F1, was isolated from soil contaminated with chlorinated hydrocarbons. This bacterium demonstrated a sustained iron reduction capability, achieving a reduction rate of 38.7% for Fe(Ⅲ) within 15 days. Genome sequencing revealed that strain F1 harbors 53 functional iron reduction genes and 2 dehalogenation genes. Single-factor experiments identified the optimal conditions for TCE degradation in soil using the coupling material: glucose concentration at 40 mmol/kg, soil water content at 50%, and bacterial inoculum at 1% (v:w). Under these optimal conditions, the coupled material achieved 86.86% degradation of TCE in soil within 28 days. Further analysis using X-ray photoelectron spectroscopy of micron iron powder, soil Fe(Ⅱ) concentration, and soil physicochemical properties demonstrated that the addition of strain F1 to the soil could disrupt the passivation layer of iron oxide on the surface of micron iron powder, promoting the exposure of its reactive sites and internal reducing active components. This resulted in an in situ self-actuated activation of passivated micron iron powder, leading to an improved removal rate and complete dechlorination of TCE in the soil. Soil microbial high-throughput sequencing revealed that the addition of strain F1 regulated the soil bacterial community, significantly enriching of Escherichia-Shigella species associated with iron-reducing functions. This enrichment facilitated the degradation of TCE in the soil through coupling materials. The functional material plays a crucial role in achieving green treatment and risk control of sites contaminated with chlorinated organic pollutants.
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高影响因子
PLANT CELL REPORTS
·
2024
·Vol. 43
·期 4
·1-15
·
IF 5.3
Ma Guanglei,Hu Bijie,Yang Siyin,Cen Zixuan,Zheng Yiran,Dong Yan
Key message The regulatory action of BXs secreted by wheat on the pathogenicity of FOF causing Fusarium wilt in faba bean were analyzed. DIMBOA and MBOA weakened the pathogenicity of FOF. Abstract A large number of pathogenic bacteria in continuous cropping soil infect faba bean plants, leading to the occurrence of wilt disease, which restricts their production. Faba bean–wheat intercropping is often used to alleviate this disease. This study investigates the effect of benzoxazinoids (BXs) secreted by wheat root on the pathogenicity of Fusarium oxysporum f. sp. Fabae (FOF) and underlying molecular mechanisms. The effects of DIMBOA(2,4-dihydroxy-7-methoxy-1,4-benzoxazine-4-one) and MBOA(6-methoxybenzoxazolin-2-one) on the activity of cell-wall-degrading enzymes in FOF(cellulase, pectinase, amylase, and protease), FOF Toxin (fusaric acid, FA) content were investigated through indoor culture experiments. The effect of BXs on the metabolic level of FOF was analyzed by metabonomics to explore the ecological function of benzoxazines intercropping control of Fusarium wilt in faba bean. The results show that the Exogenous addition of DIMBOA and MBOA decreased the activity of plant-cell-wall-degrading enzymes and fusaric acid content and significantly weakened the pathogenicity of FOF. DIMBOA and MBOA significantly inhibited the pathogenicity of FOF, and metabolome analysis showed that DIMBOA and MBOA reduced the pathogenicity of FOF by down-regulating related pathways such as nucleotide metabolism and linoleic acid metabolism, thus effectively controlling the occurrence of Fusarium wilt in faba bean.
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高影响因子
Frontiers in Microbiology
·
2024
·
IF 4
Qiannan Li,Hanyu Pan,Peng Hao,Zhenhua Ma,Xiaojun Liang,Lianyu Yang,Yunhang Gao
The 17β-estradiol (E2)-degrading bacterium Rhodococcus sp.RCBS9 previously showed remarkable resistance to the combined stresses of low temperature and E2. In this study, physiological experiments and transcriptomic analysis were performed to investigate the mechanisms underlying the strain's low-temperature adaptation and briefly analyze how it maintains its ability to degrade E2 at low temperature. The results showed that the strain's signal transduction functions, adaptive changes in cell membrane and cell wall structure, gene repair functions, and synthesis of antioxidants and compatible solutes are key to its ability to adapt to low temperature. In addition, its stress proteins in response to low temperature were not typical cold shock proteins, but rather universal stress proteins (USPs) and heat shock proteins (HSPs), among others. The strain also upregulated biofilm production, transporter proteins for carbon source uptake, and proteins for fatty acid degradation to ensure energy generation. The strain's multiple stress responses work synergistically to resist low-temperature stress, ensuring its adaptability to low-temperature environments and ability to degrade E2. Finally, six genes related to survival at low temperature (identified in the transcriptome analysis) were expressed in E. coli BL21, and they were found to contribute to recombinant E. coli growth at low temperature.
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高影响因子
BIODEGRADATION
·
2025
·Vol. 36
·期 4
·1-15
·
IF 3.2
Shao Pengfei,Yao Jun,Li Miaomiao,Min Ning
Phthalate esters (PAEs) are widely used as plasticizers and beneficiation flotation agents in ore smelters, which are ubiquitously distributed emerging contaminants in the environment. The biodegradation of PAEs by degrading microbes is a promising method for their remediation. In this study, we isolated a novel PAE-degrading bacteria, Sinomonas sp. S2, from a contaminated area of a metal(loid) smelter in Guangxi Zhuang Autonomous Region, China. Strain S2 is capable of degrading short-chain PAEs, including dimethyl phthalate (DMP), diethyl phthalate (DEP), di-iso-butyl phthalate (DIBP) and di-n-butyl phthalate (DBP). Sinomonas sp. S2 can completely degrade DBP at concentrations of 400 mg·L −1 within 24 h. The degradation kinetics of PAEs followed the modified Gompertz model. Strain S2 demonstrated good environmental adaptability thriving at pH ranging from 5 to 9 and temperatures between 20 and 40 °C, indicated by its growth on DBP. The optimal pH and temperature for degradation were found to be 7 and 40 °C, respectively. Additionally, several metabolites of DBP were identified, including phthalic acid (PA), butyl acetate, ethyl propionate and methyl 2-methylbutyrate. The reconstructed degradation pathway of DBP may involve protocatechuic acid, β-carboxy-cis, cis-mucronate and γ-carboxy muconolactone, ultimately leading to the tricarboxylic acid (TCA). In a bioaugmentation experiment involving soil artificially contaminated with DBP, strain S2 could promote the degradation of DBP in soil. The results indicate that strain S2 had high degradation capacity and environmental tolerance, which had the potential to be applied in the bioremediation of DBP-contaminated environments.
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高影响因子
Advanced Science
·
2023
·Vol. 10
·期 7
·2206236
·
IF 15.1
Zichen Wang,Jiacheng Sun,Jiye Li,Lang Wang,Zishun Li,Xiaorui Zheng,Liaoyong Wen
Bound states in the continuum (BICs) have a superior ability to confine electromagnetic waves and enhance light–matter interactions. However, the quality-factor of quasi-BIC is extremely sensitive to structural perturbations, thus the BIC metasurfaces usually require a very-high precision nanofabrication technique that greatly restricts their practical applications. Here, distinctive 2.5D out-of-plane architectures based plasmonic symmetry protected (SP)-BIC metasurfaces are proposed, which could deliver robust quality factors even with large structural perturbations. The high-throughput fabrication of such SP-BIC metasurfaces is realized by using the binary-pore anodic aluminum oxide template technique. Moreover, the deep neural network (DNN) is adapted to conduct multiparameter fittings, where the 2.5D hetero-out-of-plane architectures with robust high quality-factors and figures of merit are rapidly predicted and fabricated. Finally, owning to its large second-order surface sensitivity, the desired 2.5D hetero-out-of-plane architecture demonstrates a detection limit of endotoxin as low as 0.01 EU mL -1 , showing a good perspective of biosensors and others.
研究领域:
光学
·
光子学
·
材料科学
·
人工智能
·
纳米制造
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Marine Drugs
·
2021
·Vol. 19
·期 8
·416
·
IF 5.118
Xiaoshuang Gou,Danmei Tian,Jihua Wei,Yihan Ma,Yixue Zhang,Mei Chen,Wenjuan Ding,Bin Wu,Jinshan Tang
Marine fungi-derived natural products represent an excellent reservoir for the discovery of novel lead compounds with biological activities. Here, we report the identification of two new drimane sesquiterpenes (1and2) and six new polyketides (3–8), together with 10 known compounds (9–18), from a marine-derived fungusPenicilliumsp. TW58-16. The planar structures of these compounds were elucidated by extensive 1D and 2D NMR, which was supported by HR-ESI-MS data. The absolute configurations of these compounds were determined by experimental and calculated electronic circular dichroism (ECD), and their optical rotations compared with those reported. Evaluation of the anti-inflammatory activity of compounds1–18revealed that compound5significantly inhibited the release of nitric oxide (NO) induced by lipopolysaccharide (LPS) in RAW264.7 cells, correlating with the inhibition of expression of inducible nitric oxide synthase (iNOS). In addition, we revealed that compounds1,3–6,14,16,and18showed strongα-glucosidase inhibitory effects with inhibition rates of 35.4%, 73.2%, 55.6%, 74.4%, 32.0%, 36.9%, 88.0%, and 91.1%, respectively, which were comparable with or even better than that of the positive control, acarbose. Together, our results illustrate the potential of discovering new marine-based therapeutic agents against inflammation and diabetes mellitus.Keywords:marine-derived fungus;drimane sesquiterpene;polyketide;anti-inflammatory effect;α-glucosidase inhibitory effect
研究领域:
药理学
·
化学
·
微生物学
·
海洋生物学
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高影响因子
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY
·
2013
·Vol. 91
·期 1
·55-62
·
IF 1.592
Qin Xiao-Li,Huang Hui-Hua,Lan Dong-Ming,Wang Yong-Hua,Yang Bo
Typoselectivity of crude CBD-T1 lipase ( Geobacillus sp. T1 lipase fused with a cellulose binding domain) was investigated. Multi-competitive reaction mixtures including a set of n -chain fatty acids (C8:0, C10:0, C12:0, C14:0, C18:1 n-9, C18:2 n-6 and C18:3 n-3) and tripalmitin-enriched triacylglycerols were studied in hexane. The crude CBD-T1 lipase discriminated strongly against C18:1 n-9 [competitive factor ( α ) = 0.23] and showed the highest preference for C8:0 ( α = 1). Utilizing the catalytic properties of crude CBD-T1 lipase, acidolysis of soybean oil with C8:0 was selected as a model reaction to investigate the ability of the lipase to produce MLM-type (medium-long-medium) structured lipids. Several reaction parameters (added water amount, reaction temperature, substrate molar ratio and reaction time) examined for incorporating C8:0 into soybean oil, the optimum conditions were: 1:3 (soybean oil/C8:0) of molar ratio, 3 mL of hexane, 50 °C of temperature, 48 h of reaction time, 20 % of crude CBD-T1 lipase (w/w total substrates), and 7.5 % of water (w/w enzyme). Under these conditions, the incorporation of C8:0 was 29.6 mol%. The results suggest that crude CBD-T1 lipase, which showed different fatty acid specificity profiles, is a potential biocatalyst for the modification of fats and oils.
研究领域:
生物化学
·
食品科学
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高影响因子
CHEMOSPHERE
·
2022
·
IF 8.943
Yuzhe He,Zhangna Wang,Tianyu Li,Xingxing Peng,Yetao Tang,Xiaoshan Jia
Candida tropicalis sp. was isolated with predominant biodegradation capability to phenol compounds, even with high concentration or in acid environment. The biodegradation of phenol was evaluated at the following concentrations 10–1750 mg L −1 , the strain exhibited well biodegradation efficiency. The maximum specific growth rate was 0.660 h −1 and the specific biodegradation rates was 0.47 mg (phenol) [(mg (VSS) h] −1 . Differentially expressed genes were screened out, and results revealed a complete process of energy and carbon metabolism. The genes’ arrangements and phylogenetic information showed the unique genetic characteristics of the strain. Catabolic pathways were reconstructed and some key phenol-degrading genes were obviously upregulated, including pheA , catA , OXCT and fadA . A notable detail that CMBL encoding carboxymethylenebutenolidase was speculated to be involved in a shortened pathway of phenol biodegradation, thereby contributing to the reconstruction of the novel phenol catabolic pathway through the hydrolases of dienelactone. Finally, key enzymes were verified by the analysis of specific activity.
研究领域:
环境微生物学
·
分子生物学
·
基因组学
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高影响因子
FOOD RESEARCH INTERNATIONAL
·
2026
·
IF 8
Xiaolei Li,Wanxin Wang,Yujia Li,Fumin Ma,Cuicui Duan,Dan Li
Aronia melanocarpa , renowned as the “king of anthocyanins”, exhibits pronounced acidity, astringency, and amygdalin-derived bitterness in its berries, severely restricting its food applications. Currently, fermentation of A. melanocarpa is limited by a lack of suitable lactic acid bacteria (LABs), resulting in insufficient aroma and not delicious taste in the fermentation products. To address these, we isolated 139 LABs from fermented vegetables and screened three optimal strains of L. plantarum through acclimatization in Aronia juice and sensory evaluation. Using HS-SPME-GC–MS and GC-IMS, 143 volatile organic compounds (VOCs) were detected, among which 51 key aroma-active compounds with odor activity values (OAVs) >1 were identified. Thirty differential VOCs were screened via OPLS-DA with variable importance in projection (VIP) >1. Ten characteristic key aroma-active compounds were confirmed with the random forest algorithm. 1-butanol-3-methyl acetate, which has a fruity and sweet flavor, was a prominent marker aroma produced during fermentation. Fermentate 8–2 had the highest scores in sweet, sour, fruity, rose, whiskey and fatty. 244 non-volatile metabolites were detected by UHPLC-Q-TOF/MS, of which 50 differential metabolites were identified as markers to distinguish variations of black chokeberry fermentates. Pathway enrichment analyses revealed that phenylalanine, tyrosine, and tryptophan biosynthesis and linoleic acid metabolism, play pivotal roles in regulating volatile flavor compound formation. Syringin was first identified in black chokeberry and its LAB-fermented juices. Fermentation greatly improved the flavor and taste of the chokeberry juice. These findings provide critical theoretical support and practical guidance for the commercial production and processing of aronia and its fermentates.
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Microorganisms
·
2025
·Vol. 13
·期 9
·2033
·
IF 4.2
Yanmei Cai,Xinyu Li,Shuang Chen,Qichao Liu,Hongxiang Lu,Jiahui Xie,Wei Li,Guiying Chen
Microorganisms with chitin-degrading capabilities play a crucial role in the biological control of crop pests and diseases as well as in the treatment of organic waste. In this study, a chitin-degrading bacterium, designated L2-2, was isolated from the intestine ofOdorrana margaretaecollected in Mount Emei, Sichuan, China. Based on physiological and biochemical characteristics, 16S rRNA gene sequencing, and phylogenetic analysis of 31 conserved housekeeping genes in the whole genome, strain L2-2 was identified as a member of the genusRoseateles, namedRoseatelessp. L2-2. This strain is able to grow on agar medium with colloidal chitin as the sole carbon source and form clear hydrolysis zones. After optimizing fermentation conditions (including concentrations of nitrogen and carbon sources, culture time, and pH), the enzyme activity was increased to 3.46 U/mL, which was 24 times higher than the initial enzyme activity. Functional genome annotation showed that the strain contains genes encoding endochitinases of the GH18, GH23, and GH46 families, as well as genes encoding β-glucosidases of the GH1, GH2, GH3, and GH109 families, indicating its genetic basis for chitin-degrading potential. This study expands the diversity of known chitin-degrading bacteria and provides a promising microbial resource for the bioremediation of chitinous waste and sustainable pest control in agriculture.
研究领域:
微生物学
·
环境生物技术
·
分子生物学
·
应用微生物学
·
生物修复
·
农业微生物学
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高影响因子
JOURNAL OF NATURAL PRODUCTS
·
2022
·
IF 4.803
Jie Liu,Xiang Guo,Xingchen Guo,Boyuan Zhong,Tao Wang,Dong Liu,Hongwei Jin,Jinwei Ren,Zihe Liu,Jiangtao Gao,Shu-Ming Li,Aili Fan,Wenhan Lin
Spiromaterpenes are a group of rare tropone-containing sesquiterpenes with antineuroinflammatory activity. Herein, we elucidate their biosynthetic pathway in a deep-sea-derived Spiromastix sp. fungus by heterologous expression, biochemical characterization, and incubation experiments. The sesquiterpene cyclase SptA was first characterized to catalyze the production of guaia-1(5),6-diene, and a multifunctional cytochrome P450 catalyzed the tropone ring formation. These results provide important clues for the rational mining of bioactive guaiane-type sesquiterpenes and expand the repertoire of P450 activities to synthesize unique building blocks of natural products.
研究领域:
生物化学
·
微生物学
·
天然产物化学
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高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2021
·
IF 7.963
Hanghai Zhou,Lijia Jiang,Keliang Li,Chunlei Chen,Xiaoyun Lin,Chunfang Zhang,Qinglin Xie
This study investigated the effect of immobilized biosurfactant-producing bacteria on the bioremediation of diesel oil-contaminated seawater. Initially, a biosurfactant-producing bacterium, LQ2, was isolated from a marine cold-seep region, and identified as Vibrio sp. The biosurfactant produced by LQ2 was characterized as a phospholipid, exhibiting high surface activity with strong stability. Meanwhile, the inoculation of biochar-immobilized LQ2 demonstrated superior efficiency in removing diesel oil (94.7%, reduction from 169.2 mg to 8.91 mg) over a seven-day period compared to free-cell culture (54.4%), through both biodegradation and adsorption. In addition, the microbial growth and activity were greatly enhanced with the addition of immobilized LQ2. Further experiment showed that degradation-related genes, alkB and CYP450–1 , were 3.8 and 15.2 times higher in the immobilized LQ2 treatment, respectively, than those in the free cell treatment. The findings obtained in this study suggest the feasibility of applying immobilized biosurfactant-producing bacteria, namely LQ2, in treating diesel oil-contaminated seawater.
研究领域:
环境科学
·
微生物学
·
生物技术
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高影响因子
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