Autophagy
·
2026
·
IF 18.6
Zhengran Li,Huiqing Zhao,Danjie Zhang,Jing Wei,Xiyue Tao Liu,Ruiyan Kong,Xuejing Ren,Hang Zhao,Xiaomin Fang,Jingyan Fu,Zhouhua Li
The self-renewal and differentiation of stem cells are tightly controlled to maintain tissue homeostasis. Failure in stem cell maintenance results in stem cell depletion and precocious aging. However, how stem cells are maintained still remains not fully understood. Here, through a large-scale RNAi screen for maintenance and proliferation of adult Drosophila intestinal stem cells (ISCs), we identify several subunits of V-ATPase including Vha68-2/ATP6V1A, required for ISC proliferation, differentiation and tissue regeneration. Inactivation of Vha68-2 results in accumulation of plasma membrane (PM)-derived structures in autophagosomes and lysosomes through Atg16 and Rab5. Furthermore, Vha68-2-defective ISCs undergo direct differentiation due to ectopic Notch activation. Mechanistically, Vha68-2 facilitates Notch receptor internalization and subsequent degradation in autolysosomes to quench ectopic Notch activation, thereby maintaining ISC fate. Truncated Notch product devoid of its extracellular domain is accumulated in autolysosomes upon Vha68-2 deficiency. The Notch products accumulated in autolysosomes still require further cleavage to drive ISC differentiation. The functions of Vha68-2 in macroautophagy/autophagy and ISC maintenance are evolutionarily conserved. Our results provide new insights into the underlying mechanism of how autophagy is involved in stem cell maintenance under physiological conditions.Abbreviations: aph-1: anterior pharynx defective 1; ATP6V1A: ATPase H+ transporting V1 subunit A; CASM: conjugation of ATG8s to single membranes; EB: enteroblast; EC: absorptive enterocyte; ER: endoplasmic reticulum; ISC: intestinal stem cell; kuz: kuzbanian; LTDR: LysoTracker Deep Red; LTR: LysoTracker Red; MARCM: mosaic analysis with a repressible cell marker; NECD: Notch extracellular domain; NICD: Notch intracellular domain; PM: plasma membrane; PAS: phagophore assembly site; SNARE: soluble N-ethylmaleimide-sensitive factor-attachment protein receptor; TEM: transmission electron microscopy; TM: tunicamycin.
✓ 已验证
产品关联
DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2026
·
IF 5.4
Zhiyuan Xu,Jingming Yan,Fengyang Liu,Rui Ma,Chao Yang,Wentao Hu,Wenjie Zou
Imidazolium-based polymer (ImPOP-2) can efficiently enrich low-concentration Nb (V) in sulfuric acid-oxalate solutions. • ImPOP-2 exhibited high capacity and fast kinetics for Nb (Ⅴ) via anion exchange. • ImPOP-2 demonstrated superior selectivity towards Nb (V). • The weak interaction between Nb(V) and ImPOP-2 was revealed. Common solvent extraction methods are uneconomical for large volumes of low-concentration Nb(V) leachate, whereas ion exchange exhibits clear advantages. Nevertheless, studies on ion-exchange materials for the recovery of low-concentration niobium in non-fluoride systems are still limited, particularly in the sulfuric acid-oxalate leaching system. Emerging imidazolium-based cationic porous organic polymers have demonstrated great potential for capturing anions. However, few studies have explored their use for Nb(V) capture. In this study, a cationic organic polymer named ImPOP-2 was synthesized and used for batch adsorption experiments in sulfuric acid-oxalate solutions. ImPOP-2 exhibited a high adsorption capacity (212.20 mg·g⁻¹), rapid kinetics (reaching 79.66% of the equilibrium adsorption capacity within 10 min at an initial concentration of 400 mg·L⁻¹) and good reusability (maintaining 91.62% of the original capacity following five regeneration cycles). Even in the presence of 10 times higher concentrations of Fe(III) and 2 times higher concentrations of Ti(IV), ImPOP-2 exhibited good selectivity for Nb(V) over these competing ions, with selectivity coefficients (adsorbed mass ratio) of 1.27 and 1.67, respectively. ImPOP-2 demonstrated superior adsorption performance compared to existing adsorbents used in non-fluoride systems. The interactions between ImPOP-2 and Nb(V) were analyzed using scanning electron microscopy, X-ray photoelectron spectroscopy, Fourier Transform infrared spectroscopy and independent gradient model based on Hirshfeld partition analysis. The results indicated that the adsorption mechanism was primarily ion exchange driven by electrostatic forces, with van der Waals forces and hydrogen bonding also contributing to the adsorption. This study aims to provide a feasible strategy for the efficient enrichment of low-concentration niobium in the sulfuric acid-oxalate leaching system. Download: Download high-res image (214KB) Download: Download full-size image
✓ 已验证
产品关联
DOI
高影响因子
PHYTOMEDICINE
·
2026
·
IF 11.3
Deyu Zuo,Dingyi Lv,Guozhi Zhao,Yanliang Chen,Zhesheng Chen,Lili Pu,Minghao Luo,Ming Tang,Ming Wang,An He,Weijian Xiong,Xunjia Li
Background Vascular endothelial dysfunction is a core pathological feature of diabetes-associated vascular complications, primarily driven by impaired nitric oxide (NO) bioavailability due to dysregulated endothelial nitric oxide synthase (eNOS) activity. While pharmacological strategies targeting the eNOS pathway have shown promise, the discovery of natural compounds capable of restoring endothelial function through novel molecular targets remains an important research direction. Purpose The aim of this study was to investigate the targets and molecular mechanisms of Chikusetsusaponin V (CsV) in regulating vascular endothelial function in diabetes mellitus (DM). Methods The DM models were constructed using db/db mice and high-glucose cultured human aortic endothelial cells (HAECs). The vasodilatory function was investigated using techniques such as metal wire microvascular tension measurement and ultrasonography after intervention with CsV. Modern techniques such as affinity chromatography, mass spectrometry, molecular docking and microscale thermophoresis (MST) test were used to identify the direct targets of CsV. The key pathways and downstream transcription factors involved in the improvement of vasodilatory function of CsV were screened using RNA-Seq. Subsequent validation was performed using a dual luciferase assay. Knockdown adenoviral vectors, quantitative polymerase chain reaction (qPCR), and Western blot (WB) were used to elucidate the molecular mechanism of CsV in regulating the vasodilatory function in DM. Results The results showed that CsV down-regulated blood glucose and increased the expression of eNOS and p-eNOS (S1177), which improved vasodilatation and reduced vascular stiffness in DM mice. Additionally, CsV increased NO release and tube-forming capacity of HAECs. CsV can directly bind to the membrane protein ITGB1. In vivo and in vitro experiments showed that administration of CsV after knockdown of ITGB1 or inhibiting PI3K significantly down-regulated the expression of p-PI3K/PI3K, p-AKT/AKT, p-mTOR/mTOR, NR4A3, PURA, and p-eNOS (S1177)/eNOS, decreased NO levels, and affected angiogenesis and endothelial cell function. In contrast, overexpression of ITGB1 produced the opposite results. Moreover, knockdown NR4A3 inhibited NR4A3/PURA/eNOS expression and affected endothelial function, whereas overexpression produced the opposite result. Conclusions CsV improves endothelial function in DM vasculature by targeting ITGB1, enhancing eNOS phosphorylation via the PI3K/AKT pathway, and promoting PURA/eNOS expression through PI3K/AKT/mTOR/NR4A3. These findings support CsV's potential use for improving diabetes vascular function. CsV Targets ITGB1 to Improve Endothelial Function via activating PI3K/AKT/mTOR/NR4A3/eNOS Pathway - present study vs. previous study. Download: Download high-res image (224KB) Download: Download full-size image
✓ 已验证
DOI
PubMed
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2026
·
IF 19.9
Shuxia Wei,Xuejun Xu,Zongjie Su,Hongyi Lu,Yue Liu,Han Xu,Qianqian Gao,Yunfeng Wang,Ming Li,Tianyu Xing,Siyu Wang,Haoran Li,Li Wu,Yi Zhang
The open-circuit voltage ( V OC ) deficit remains a primary bottleneck hindering the efficiency of solution-processed Cu(In,Ga)(S,Se) 2 (CIGSSe) solar cells, largely due to the uncontrolled elemental diffusion during high-temperature selenization that prevents the formation of a beneficial bandgap gradient. Here, we propose a novel and highly effective low-temperature surface treatment strategy to overcome this long-standing challenge. By spin-coating a gallium-thiourea complex solution onto the pre-fabricated CIGSSe absorber, we construct a strategic Cu-poor, Ga-rich surface layer. Such design widens the surface bandgap, leading to an optimized band alignment at the CIGSSe/CdS heterojunction with a reduced conduction band offset (0.15 eV) and an enlarged valence band offset (1.13 eV). This synergistic effect drastically suppresses interface recombination. As a consequence, we achieve a champion device with a V OC of 0.692 V—the highest value reported to date for solution-processed CIGSSe—and a power conversion efficiency of 16.65% (17.93% with an MgF 2 anti-reflection layer). This work presents a simple yet powerful pathway for targeted front interface engineering, paving the way for high-efficiency, low-cost chalcopyrite photovoltaics.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2025
·
IF 9.7
Xin Li,Yue Dong,Zhixin Dai,Luyao Zhang,Bohan An,Ning Li,Yangqin Gao,Lei Ge
Anion exchange membrane water electrolysis (AEMWE) is regarded as a highly promising technology for industrial hydrogen production. The development of efficient and stable anodic oxygen evolution reaction (OER) catalysts is crucial for advancing this technology. In this work, a heterojunction catalyst was constructed by means of the vertical anchoring of NiFe LDH onto larger CoFeP nanosheets. This heterojunction structure modulates the electronic structure, thereby enhancing conductivity and optimizing the efficiency of adsorption/desorption of reaction intermediates. Furthermore, the hierarchical nanosheet morphology facilitates bubble detachment and exposes more active sites, collectively enhancing the electrocatalytic activity. The CoFeP@NiFe LDH electrode requires an overpotential of only 268 mV to achieve a current density of 100 mA cm −2 and maintains stability for 100 h, demonstrating superior performance to most reported NiFe LDH catalysts. When employed in an anion exchange membrane (AEM) electrolyzer with PtC as the cathode, the CoFeP@NiFe LDH anode achieved a current density of 1.0 A cm −2 at a cell voltage of just 1.67 V, significantly outperforming the benchmark RuO 2 || PtC system. This work provides a practical strategy for designing high-performance anodic catalysts for advanced AEMWE systems.
研究领域:
电催化
·
氢能
·
材料科学
·
可再生能源
·
纳米材料
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Environmental Chemical Engineering
·
2025
·
IF 7.2
Meiqing Chen,Wanxia Li,Bo Ruan,Yuxin Gu,Qingcheng Zhang,Hongling Bu,Peixin Du,Peng Yuan
The interaction between birnessite and dissolved organic matter(DOM) is critical for carbon sequestration and heavy metal immobilization. This study investigated humic acid (HA, a representative form of DOM) in mediating the formation of triclinite birnessite (TB) and elucidated the influence mechanisms for carbon sequestration and As(V) immobilization. HA significantly affected both the growth and structural properties of TB. HA induced TB structural transformation from lamellar-like to honeycomb-like, reduced surface negative charge, and increased specific surface area, oxygen vacancies and hydroxyl groups. HA-Mn(III) complexes and pore trapping enhance organic carbon sequestration synergistically. As(V) immobilization was improved by inner-sphere complexation (via hydroxyl coordination) and electrostatic attraction with the maximum adsorption capacities for HA-mediated birnessite (TB-2H) were 65.140 mg·g⁻¹ , 6.4-fold higher than pure TB. Besides, TB-2H shows low manganese release, high stability and good reusability. These findings achieves synergistic carbon sequestration and As(V) immobilization, providing an innovative strategy for environmental remediation.
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Energy Materials
·
2025
·
IF 5.5
Zhengde Wang,Zhengfeng Zhang,Xingkai Zhang,Rupeng Li,Yunxia Wang,Jin Li,Bin Zhang
The LiCoO2 cathode cannot maintain stable battery cycling under high voltage, as the undesired release of lattice O and harmful surface side reactions compromise its structural stability, resulting in battery capacity degradation. Herein, inspired by architectural engineering, we proposed a surface–bulk structure dual-reinforced strategy to tackle the aforementioned challenges by room-temperature Al-induced engineering, which could achieve surface disordering and phosphating and bulk gradient Ni doping of the LiCoO2 cathode. Such a multifunctional structure could delay the detrimental transition, restrain lattice O loss, reduce harmful surface side reactions, and form a phosphate-rich CEI with high ionic conductivity and stability. The as-prepared modified LiCoO2 as the cathode in lithium-ion batteries displayed remarkably enhanced cycle stability than bare LiCoO2 (91.9% vs 45.3% after 100 cycles in 3.0–4.6 V at a current density of 1 C). Even at a 5 C high rate, the modified LiCoO2 cathode also displayed superb battery cycling (92% after 100 cycles at 3.0–4.6 V). This room-temperature Al-induced engineering is universal to other layered cathode materials.
研究领域:
材料科学
·
电化学
✓ 已验证
产品关联
DOI
高影响因子
ADVANCED MATERIALS
·
2025
·
IF 26.8
Yuqing Li,Weixing Xiong,Qunting Qu,Jie Shao,Ying Yan,Ru Wang,Linze Lv,Honghe Zheng
Dual-ion batteries (DIBs) composed of a graphite cathode and a lithium anode are promising candidates for high-energy and high-power energy storage systems. However, graphite cathode undergoes rapid failure during the extended cycling and rapid charge/discharge mainly because of its structural breakdown and drastic resistance rise of cathode/electrolyte interphase (CEI) arising from the violent electrolyte decomposition at high voltage (4.5–5.0 V). Unlike the mainstream CEI modification strategy solely solving the problem of electrolyte decomposition, this work proposes a bifunctional CEI construction strategy that not only inhibits the electrolyte decomposition but also enhances the mechanical stability of graphite cathodes. Three pH-variable phosphates (LiH 2 PO 4 , Li 2 HPO 4 and Li 3 PO 4 ) are artificially coated on the surface of natural graphite (NG) particles through a green and low-cost wet coating route. The acidic LiH 2 PO 4 coating not only effectively suppresses the electrolyte decomposition through the formation of a conformal coating layer, but also considerably enhances the mechanical strength of NG cathode via a strong bonding between LiH 2 PO 4 and binder. The underlying mechanisms are elucidated through both theoretical calculations and empirical experiments. The optimized NG cathode is able to withstand fast charge/discharge at 60 C and exhibits exceptional capacity retention of 80.7% after 10,000 cycles 2 C.
研究领域:
储能与转换
·
电化学
·
材料科学
·
电池技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Advanced Energy Materials
·
2023
·Vol. 13
·期 18
·2203999
·
IF 27.8
Lifan Wang,Guicheng Liu,Rui Xu,Xindong Wang,Liguang Wang,Zhenpeng Yao,Chun Zhan,Jun Lu
The Ni-rich layered oxide cathode is pushing the frontier of battery powered electric vehicles toward longer driving range and lower cost, whilst facing a major challenge with the compromised cycle life and thermal robustness. It is well recognized that irreversible oxygen evolution at the cathode-electrolyte interphase is critical to the electrochemical and thermal stability of the Ni-rich cathode. Herein, combining experiments with density functional theory (DFT) calculations, the authors focus on manipulating the irreversible oxygen evolution to solve the performance degradation and safety hazard. An oxygen ion conductor introduced to the surface of the cathode restrains the activated surficial lattice oxygen ions by its stable oxygen vacancies. Meanwhile, a Li-rich fast ion conductor incorporated in the coating layer synergistically reinforces the Li diffusion path through the cathode-electrolyte interphase. This sophisticated multifunctional surficial modification implemented by a neat one-step treatment represents a successful design and development of a thermally stable Ni-rich cathode and approximately 400-cycle state-of-health up to 80% with the operating voltage range extended up to 4.5 V. Therefore, this study provides an encouraging strategy to overcome the capacity versus robustness dilemma of high-energy cathodes.
研究领域:
材料科学
·
电化学
·
能源存储
·
纳米材料
·
固体离子学
·
电池技术
·
表面工程
✓ 已验证
DOI
高影响因子
Energy Storage Materials
·
2024
·
IF 18.9
Meng Wang,Xiaolang Liu,Wen Zhang,Hongwei Niu,Leimin Xu,Jiantao Han,Yuhao Lu
For the first time, this work investigates the synergetic impact of yttrium (Y) and aluminium (Al) co-doping on the electrochemical properties of 4.6 V LiCoO 2 (LCO) cathode for high energy density lithium-ion batteries. The optimized LCO demonstrates an impressive initial discharge capacity of 208.6 mAh g −1 in the voltage range of 3–4.6 V, along with superior structural stability , retaining over 86 % of its initial capacity even after 125 cycles at the current of 100 mA g −1 that is far higher than the capacity retention of 40 % for the pristine LCO under the same condition. Thanks to the synergistic effect , Al in Co layers enhances the bonds of Al/Co-O to stabilize oxygen in the LCO crystal structure while Y serves “pillar” in Li layers, migrated from the initial Co layers during cycling process, effectively curtail the formation and expansion of interlayer cracking, which contributes to the long-term cycle stability of LCO under high voltage. Most importantly, Al/Y co-doping is a facile process compatible with the existing industrial infrastructure for LCO mass production, and thus it can be scaled up readily for commercial applications. Al/Y co-doped LCO is assembled into commercial-level pouch cells with graphite anode, achieves an extremely high energy density of 750 Wh/L, and exhibits a superior cycling stability and obtains a capacity retention of 88.2 % at 25 °C after 800 cycles with the high cutoff voltage of 4.55 V.
研究领域:
材料科学
·
电化学
·
储能
·
电池技术
✓ 已验证
DOI
高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2026
·Vol. 37
·期 4
·318
·
IF 3.2
Su Xiuyuan,Chen Yuan-Bin,Tang Ruxuan
Based on the Na 0.48 La 0.52 MoO 4 ceramic, this study replaced La 3+ ions with Nd 3+ ions through ion substitution, which significantly increased the Q × f of Na 0.48 La 0.52 MoO 4 and reduced the sintering temperature. The good sintering state of this series of ceramics was demonstrated by scanning electron microscopy (SEM) and density tests. X-ray diffraction (XRD) and Raman spectroscopy results revealed the formation of solid solutions without the presence of secondary phases or impurities. The P–V–L theory indicated that the Na/La/Nd–O bond-to-bond ionicity (fi) contribution was dominant, while the Mo–O bond also played a significant role in the lattice energy ( U ), bond energy ( E ), and thermal expansion coefficient ( α ). Among them, the Na 0.48 La 0.48 Nd 0.04 MoO 4 ceramic achieved the best dielectric performance at 900 °C, with ɛ r = 11.027 ± 0.018, Q × f = 113,805 ± 6,358 GHz, and τ f = − 23.36 ppm/℃. Through ion substitution, these materials all have low sintering temperatures and high Q × f values, making them ideal candidates for low-temperature co-fired ceramics (LTCC).
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2026
·Vol. 37
·期 4
·287
·
IF 2.8
Tang Ruxuan,Chen Yuan-Bin,Su Xiuyuan,Tang Ling
With the rapid development of 5G/6G mobile communication technology, microwave dielectric materials are facing increasingly strict performance requirements. As core fundamental materials for modern communication systems, performance optimization of microwave dielectric ceramics has become a research hotspot. Addressing the limitations of single-phase ceramic materials, dense microwave dielectric ceramics of (Nd 1- x La x ) 2 [Zr 0.89 (Bi 0.5 Ta 0.5 ) 0.11 ] 3 (MoO 4 ) 9 ( x = 0.01, 0.03, 0.05, 0.07) were prepared by the conventional solid-state reaction method. The experimental process involved 550 °C pre-sintering followed by gradient sintering at 600–725 °C (4 h holding time). X-ray diffraction (XRD) analysis confirmed that all samples exhibited trigonal crystal structure with \(R\overline{3} c\) space group. The lattice parameters were obtained by Rietveld refinement method. Scanning electron microscopy (SEM) characterization indicates that compactness has a significant impact on the dielectric constant ( ε r ) and the quality factor ( Q × f ). Raman spectroscopy analysis indicated strong associations of ε r and Q × f values with characteristic peak shifts and full width at half maximum ( FWHM ), respectively. When sintered at 675 °C, the NLZBTM ( x = 0.03) ceramics exhibited exceptional comprehensive performance: ε r = 11.24 ± 0.02, Q × f = 116,914 ± 5738 GHz, and τ f = − 37 ± 1.5 ppm/℃. The bond property analysis based on the P–V–L theory shows that the ionicity of the Nd/La–O bond makes the greatest contribution to εᵣ , while the covalency of the Mo–O bond significantly affects the Q × f value and τ f parameter. It is noteworthy that this material achieves low-temperature sintering at 675 °C while maintaining excellent microwave dielectric properties, demonstrating significant potential as a candidate material for low-temperature co-fired ceramics (LTCC).
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）