INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Penghui Zhu,Bochen Pan,Chen Li,Benwei Zhu,Zhong Yao,Yun Sun
Green algal polysaccharides such as ulvan and Enteromorpha polysaccharides (EPs) with versatile physiological activities have drawn increasing attentions as a renewable biomass resource. However, their activities and applications have been greatly limited by its high molecular weight and low solubility. Herein, a novel polysaccharide lyase (PL) family 25 ulvan lyase, termed EPD2, has been identified, heterologously expressed and biochemically characterized to efficient preparation of ulvan and Enteromorpha oligosaccharides. It consisted of 446 amino acids with a molecular weight of 50.28 kDa and shared the highest identity with the ulvan lyases of PL25 family. The EPD2 exhibited optimal activity (1764.95 U/mg and 179.9 U/mg for ulvan and EPs, respectively) at 50 °C. It also exhibited robust salt tolerance and could maintain high activity under high NaCl concentrations (0.4–1.2 M). The molecular docking results indicated that EPD2 produce oligosaccharides with degrees of polymerization (DPs) of 2–4 in an endolytic manner. To expand its applications, EPD2 was then immobilized onto sodium alginate and its structure was characterized. After immobilization, its stability at 40 °C was significantly enhanced. This research enriched the enzyme tools library for degrading Enteromorpha polysaccharide and facilitates the expanded application of these enzymes in preparation of oligosaccharides.
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PubMed
高影响因子
Journal of the Taiwan Institute of Chemical Engineers
·
2019
·
IF 3.834
Wenjing Cheng,Yun Li,Xiaoying Li,Wenjing Bai,Yunxiao Liang
A monolithic macroporous silica (MMS) was prepared by using a pillared epoxy resin macroporous polymer as template. Polydopamine (PDA) as a spacer-arm polymer was modified on the surface of MMS for immobilization of Candida rugosa lipase (CRL), aiming to improve the performance of CRL and overcome the limitations of the immobilized enzymes on mesoporous materials and fine particles. The immobilization conditions and the properties of the immobilized CRL were investigated. The as-prepared monolithic macroporous materials were characterized by SEM, TEM, MIP, BET, FT-IR and TG-DTA. The results revealed that MMS possessed two sets of well-defined and uniform continuous macroporous channels (pore size of 300–500 nm), high mechanical strength, large pore volume and specific surface area, and that the PDA/MMS macroporous composite was constructed by PDA/SiO 2 /PDA nano-film with a thickness of about 50 nm. Because of its surface chemical properties and unique macropore structure, the PDA/MMS composite provided a simple, cost-effective way to enhance the catalytic activity of directly immobilized enzyme. The activity recovery of CRL–PDA/MMS was 201%, and the stability of the immobilized lipase was remarkably improved. Moreover, this biocatalyst can be easily recovered from the reaction system without centrifugation or filtration.
研究领域:
生物化学
·
材料科学
·
酶工程
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高影响因子
CHEMOSPHERE
·
2022
·
IF 8.943
Yongqiang Yang,Xiongfei Rao,Qingling Fu,Xin Zhang,Jieyu Gao,Xiang Wan,Jun Zhu,Guoyong Huang,Hongqing Hu
Organic acids usually compete the immobilization of As by iron (hydro)oxides, but their oxidizing effects are ignored. Therefore, the gallic acid (GA) with strong redox activity was chosen to investigate the influence of arsenite [As(III)] oxidation on As immobilization by ferrihydrite . Our results found that the As amount adsorbed on ferrihydrite decreased with the pH rising from 5 to 9 in the presence of GA, and the adsorption amount (28.8 g kg −1 ) at pH 9 was 45.1% lower than that in the absence of GA. Meanwhile, the As adsorption amounts in treatments of GA addition before As (Fh-GA-As(III)) were significantly lower than that in their corresponding simultaneous addition (Fh-As(III)/GA). The proportions of As(V)/As total on ferrihydrite and in equilibrium suspension were increased as the pH increased in the presence of GA, and the highest oxidation efficiency of As(III) by GA at pH 9 was 90.3%, which was mainly due to the contribution of hydrogen peroxide (H 2 O 2 , 52.6%) and semiquinone radicals (SQ − , 27.1%). In general, the oxidation and competition adsorption of As by GA inhibited the As immobilization by ferrihydrite, and the oxidation of As(III) by GA was strongly dependent on pH, while H 2 O 2 and SQ − were demonstrated as the main oxidant at pH 9.
研究领域:
环境科学
·
土壤学
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高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2026
·
IF 8.5
Guangai Sun,Shijie Guo,Yuanyuan Yao,Zeyu Lin,Hui Gao,Roland H. Stauber,Bin-Chun Li,Guo-Bin Ding
α-L-Rhamnosidase is a significant biocatalyst that specifically cleaves terminal α-L-rhamnose group from natural flavonoid diglycosides, enabling the biocatalytic production of high-value flavonoid glucosides like prunin from naringin. Compared to its precursor naringin, prunin exhibits superior solubility and bioavailability, making this enzymatic conversion commercially valuable for the food and pharmaceutical industry. However, the industrial application of free α-L-rhamnosidases faces significant challenges, including enzyme instability, difficulty in recovery, and unsatisfactory reusability. To address these issues, this study developed an innovative magnetically recoverable biocatalyst (Fe₃O₄-Rha) through covalent immobilization of recombinant α-L-rhamnosidase from human fecal metagenome onto Fe₃O₄ nanoparticles using EDC/NHS chemistry, and the immobilization parameters were systematically optimized. The successful preparation of Fe₃O₄-Rha was verified by TEM, FTIR, TGA, and SQUID analysis. Fe₃O₄-Rha retained the catalytic property of free Rha in terms of optimal pH and temperature but exhibited superior tolerance on organic solvent especially ethanol and isopropanol. Moreover, Fe₃O₄-Rha could effectively biotransform naringin to prunin and maintained 61.34% of initial activity after 5 cycles. In a scaled-up reaction system, Fe₃O₄-Rha also efficiently converted naringin into prunin and the complete conversion was achieved within 10 h. This work successfully developed a magnetically recoverable immobilized α-L-rhamnosidase system for the efficient and reusable biotransformation of naringin, offering a promising approach for the enzymatic modification of bioactive small molecules using biological macromolecular catalysts.
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高影响因子
Bioresource Technology Reports
·
2022
·
IF 0
Jiali Mi,Simin Liu,Yan Du,Haishan Qi,Lei Zhang
Cadaverine (1,5-diaminopentane) has widespread applications in industry, agriculture and medicine. Cadaverine can be biotransformed from l -lysine by lysine decarboxylasey which is a pyridoxal 5′-phosphate (PLP)-dependent enzyme. Herein, barium alginate microspheres were fabricated by electro-dripping and employed as immobilized metal ion affinity chromatography matrix to purify and immobilize engineered lysine decarboxylase CadA-EK. Besides, polyethyleneimine (PEI) was employed for co-immobilizing PLP and enzymes (cCadA-EK-PEI/PLP). The cCadA-EK-PEI/PLP can produced 184.53 g/L cadaverine with yield of 90.03% in 2 mol/L lysine solution. And the stability of co-immobilized CadA-EK was 1–4 times higher than that of iCadA-EK + PLP in environmental factors, and the catalytic efficiency k cat / K m was increased by 3.4-fold. Furthermore, a continuous flow microreactor with the co-immobilized enzymes and cofactors was developed to catalyze lysine, with 14.28 g/(L·h) productivity of cadaverine, which displayed great potential for cadaverine biosynthesis. This work provides alternative strategy for the one-step purification and co-immobilization of enzyme and cofactor.
研究领域:
生物催化
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酶工程
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生物合成
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材料科学
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DOI
JOURNAL OF THE AMERICAN CERAMIC SOCIETY
·
2023
·Vol. 106
·期 11
·7009-7018
·
IF 3.9
Yong Ge,Baoliang Xu,Yiliao Liao,Xueyu Zhang,Xu Yan,Caohui Zheng,Tao Duan
Multiphase ceramics have a high application potential in the field of nuclear waste immobilization because of the efficient synergy between the individual phases. In this work, zirconia/garnet multiphase ceramics with high efficient and effective immobilization capacity of trivalent actinides were successfully prepared using conventional solid-phase sintering. ZrO 2 doping increases the lattice site available for nuclide substitution and advance the immobilization capacity of the ceramic substrate for nuclide. At the same time, the phase change of zirconia is used to tougher and improve the physical properties of the ceramic waste form. The feasibility of using multiphase ceramics for the efficient immobilization of trivalent actinides was assessed by studying the phase evolution, microstructure, Vickers hardness, and chemical stability of the multi-phase ceramics. Zr 1- x Nd x O 2- x /2 /Ca 3- y Nd y Zr 3- y Fe y -1 Fe 3 O 12 multiphase ceramics exhibited superior physical or chemical properties compared to most of the multiphase ceramics as well as the previously prepared single-phase calcium garnet ceramic waste forms. The Vickers hardness of all the multiphase ceramic samples ranged between 685 and 730 HV0.5. Under the condition of using simulate Bei Shan groundwater as leaching agent, the normalized leaching rates of Nd is approximately 10 − 6 g · m −2 · day −1 , showing good chemical stability and achieving efficient and effective immobilization of trivalent actinides. The excellent chemical, as well as physical properties of Zr 1- x Nd x O 2- x /2 /Ca 3- y Nd y Zr 3- y Fe y -1 Fe 3 O 12 multiphase ceramics are expected to make them one of the substrates for more efficient and effective immobilizing of trivalent actinides.
研究领域:
材料科学
·
核废料管理
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高影响因子
MICROPOROUS AND MESOPOROUS MATERIALS
·
2026
·
IF 4.7
Baomin Wang,Haoxiang Yang,Chengcheng Fan,Fei Liu,Wenxin Lu
Alkali-activated materials show excellent capture capacity on heavy metals, but the mechanisms to immobilize heavy metals remain inadequately explored. This work investigated the main hydration product C-A-S-H gels for encapsulation of heavy metals such as Pb(II), Zn(II) and Cd(II). Furthermore, the adsorption behavior and mechanisms of Pb(II) in pore solutions by C-A-S-H were examined. These findings reveal that aluminum is incorporated into the silicon chains of C-S-H in the form of aluminum-oxygen tetrahedra, thereby generating C-A-S-H. The bonding ratio, which depends on the Ca/Si ratio, reaches its peak value of 0.139 when the Ca/Si ratio is 0.9. In highly alkaline environments, amphoteric Pb(II) and Zn(II) form hydroxyl coordination polyhedra, enhancing silicon chain polymerization, whereas Cd(II) is immobilized via ion exchange with Ca(II), resulting in solid solutions. For C-A-S-H with a Ca/Si ratio of 0.6, Pb(II) adsorption occurs predominantly through physical interactions. However, as the Ca/Si molar ratio increases, the effect chemisorption and ion exchange become more significant along with the formation of Pb 8 Ca(Si 2 O 7 ) 3 . C-A-S-H gel with a Ca/Si ratio of 0.9 has the highest Pb(II) adsorption capacity, reaching 92.55 mg/g.
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高影响因子
POLYMER BULLETIN
·
2014
·Vol. 71
·期 11
·2923-2944
·
IF 1.491
Yan Huiqiong,Chen Xiuqiong,Wu Tiantian,Feng Yuhong,Wang Chunxiu,Li Jiacheng,Lin Qiang
The surface modification with poly(methyl hydrosiloxane) (PMHS) using a grinding method as a pesticide carrier had been successfully achieved. The characteristics of the modified kaolin were represented by Fourier transform infrared, X-Ray diffraction, BET specific surface area measurements, laser particle size analysis, transmission electron microscopy and contact angle measurement. Subsequently, immobilization of acetamiprid onto clay’s surface was carried out by means of freeze-drying technology. And the drug-loaded clays were encapsulated in alginate-chitosan composite beads by extrusion into calcium chloride solution. The composite beads showed regularly spherical shapes and uniform sizes with diameters of 1.44 ± 0.24–1.75 ± 0.19 mm. The immobilization and release of acetamiprid were carried out to examine the effect of the modified kaolin on the properties of the composite bead. The results showed that the intensive force of superfine pulverization during the grinding process was effective to increase the specific surface areas of the modified kaolin with particle size reduction. The modified kaolins as promising carrier materials had improved the drug loading rate and encapsulation efficiency from 4.15 and 42.01 % for alginate-chitosan composite beads to 6.02 and 82.95 %, which were higher than 4.33 and 54.29 % for SA-CS-kaolin composite beads, respectively. The controlled-release formulation containing the modified kaolin had shown slow and sustained release properties to promote the efficient use of organic pesticide to reduce pollution effect on the environment.
研究领域:
材料科学
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农业化学
·
药物递送系统
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环境友好材料
·
粘土矿物加工
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高影响因子
WATER SCIENCE AND TECHNOLOGY
·
2017
·Vol. 76
·期 1
·147-154
·
IF 1.197
Zhou Wen,Guan Zheng-Bing,Chen Yu,Zhang Feng,Cai Yu-Jie,Xu Cheng-Wen,Chen Xu-Sheng,Liao Xiang-Ru
Given that spore laccase from the Bacillus genus is heat- and alkali-resistant, it is more suitable for industrial applications than fungal laccase. To determine the optimal culture conditions for spore laccase production, the effects of Cu 2+ concentration, oxygen content, and culture time on spore laccase production from Bacillus pumilus W3 were investigated. The optimal production parameters were 0.2 mM of Cu 2+ , 200 rpm shaking speed, 100 mL liquid loading, and 5 days of cultivation. Spore laccase was efficiently immobilized on amino-functionalized celite. When used in dye decolorization, the immobilized spore laccase removed 84.15% of methyl green and 69.70% of acid red 1 after 48 h of treatment. Moreover, the immobilized spore laccase retained 87.04% of its initial decolorization activity after six cycles in the decolorization of acid red 1. These insights into the culture conditions and immobilization of spore laccases should be useful in the development of spore laccase as a biocatalyst in the treatment of textile wastewater.
研究领域:
生物催化
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环境生物技术
·
酶工程
·
工业微生物学
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DOI
高影响因子
Frontiers in Bioengineering and Biotechnology
·
2021
·
IF 5.89
Ming Zhang,Xudong Shi,Hai Sun,Donghua Xu,Yang Gao,Xi Wu,Jianqi Zhang,Jichang Zhang
The coverage of stents with healthy endothelium is crucial to the success of cardiovascular stent implantation. Immobilizing bioactive molecules on stents is an effective strategy to generate such stents. Glycogen synthase kinase-3β inhibitor (GSKi) is a bioactive molecule that can effectively accelerate vascular endothelialization. In this work, GSKi was covalently conjugated on 316L stainless steel through polydopamine to develop a stable bioactive surface. Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and water contact angle results revealed the successful introduction of GSKi onto 316L stainless steel. The GSKi coating did not obviously affect the hemocompatibility of plates. The adhesion and proliferation of human coronary artery endothelial cells (HCAECs) on stainless steel was significantly promoted by the addition of GSKi. In summary, this work provides a universal and stable strategy of immobilizing GSKi on the stent surface. This method has the potential for widespread application in the modification of vascular stents.
研究领域:
生物医学工程
·
心血管器械
·
表面改性
·
组织工程
·
生物材料
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DOI
高影响因子
Green Synthesis and Catalysis
·
2026
·
IF 7
Siqi Zhu,Jie Sheng,Yuhan Li,Kunyi Tong,Chunjie Wu,Chenhao Zhang,Hui Li
Enzymes catalyze stereoselective transformations under mild conditions, yet their industrial deployment remains constrained by intrinsic fragility, poor recyclability, and prohibitive cost. While immobilization on solid carriers can circumvent these limitations, conventional methods suffer from enzyme leaching or diffusional barriers. Covalent organic frameworks (COFs) offer an ideal solution by combining high surface area, tunable pore environments, and robust covalent linkages; however, the narrow pore apertures of conventional two-/three-dimensional scaffolds (< 2 nm) preclude efficient loading of enzymes whose hydrodynamic diameters typically exceed 5 nm. Here we demonstrate that a one-dimensional, pyrene–phenanthroline COF (PhenCOF) overcomes this steric mismatch by presenting densely distributed, surface-exposed imine groups that serve as anchoring points for covalent enzyme attachment. Candida antarctica lipase B (CALB) was immobilized via an Ugi-type three-component reaction to afford CALB@PhenCOF. The resultant hybrid exhibits exceptional catalytic performance in the kinetic resolution of racemic 1-phenylethanol, achieving 99% conversion, 2.4-fold higher than free CALB. The 1D architecture and hydrophobic microenvironment facilitate substrate diffusion while stabilizing the enzyme's active conformation, as evidenced by retention high activity under wide pH ranges, extreme organic solvents, and temperatures up to 140°C. Notably, covalent immobilization endows unparalleled recyclability, with 95% efficiency maintained over 10 cycles. This work establishes 1D COFs as a paradigm for promising enzyme carriers, merging atomic precision with industrial robustness to address longstanding challenges in biocatalysis.
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高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2026
·
IF 12.5
Weijie Ren,Guojin Yu,Kaiyi Pan,Wenhao Yang,Qixia Zhang,Yuanhang Zhou,Yueqiang Zhao,Ye Xiong
Dual-network (DN) design is an effective means of constructing radionuclide-capturing aerogels with both high adsorption capacity and excellent stability. However, the fundamental role of DN structures in regulating the dispersion of encapsulated nanoparticles and the interfacial microenvironment remains unverified. Here, the synergistic immobilization and enhanced adsorption mechanism of CuFe-Prussian blue nanoparticles (CuFe-PB NPs) by a cellulose nanofiber/polyacrylamide dual network (CNF/PAM DN) were explored through experiments and simulations. The results show that PAM tightly encapsulates the PB/CNF composite via hydrogen bonding and physical entanglement, achieving a synergistic enhancement of the material's mechanical strength and structural stability. The resulting CNF/PAM DN - engineered PB aerogel (PB/CNF DN-A) retains 90% shape recovery after 100 compression cycles. Furthermore, molecular dynamics simulations elucidate that the DN structure provides ordered ion-transport channels and electrostatic synergy. Its unique “ion pre-enrichment” and “dehydration-assisted” effects effectively lower the energy barrier for Cs + removal within the hydration layer, enhancing the adsorption kinetics of Cs + . The optimized PB/CNF DN-A significantly reduced CuFe-PB NPs leakage and achieved a Cs + adsorption capacity of up to 403.9 mg/g within 50 min. In summary, this work deepens the understanding of interface-mediated interactions in multifunctional aerogels and provides a scalable platform for high-performance environmental remediation materials.
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危险品化学品经营许可证（带存储）