Microbiology Spectrum
·
2022
·Vol. 10
·期 4
·
IF 9.043
Mohamed Sharaf,Alaa H. Sewid,H. I. Hamouda,Mohamed G. Elharrif,Azza S. El-Demerdash,Afaf Alharthi,Nada Hashim,Anas Abdullah Hamad,Samy Selim,Dalal Hussien M. Alkhalifah,Wael N. Hozzein,Mohnad Abdalla,Taisir Saber
Surface-growing antibiotic-resistant pathogenic bacteria such as Escherichia coli and Staphylococcus aureus are emerging as a global health challenge due to dilemmas in clinical treatment. Furthermore, their pathogenesis, including increasingly serious antimicrobial resistance and biofilm formation, makes them challenging to treat by conventional therapy. Therefore, the development of novel antivirulence strategies will undoubtedly provide a path forward in combatting these resistant bacterial infections. In this regard, we developed novel biosurfactant-coated nanoparticles to combine the antiadhesive/antibiofilm properties of rhamnolipid (RHL)-coated Fe3O4 nanoparticles (NPs) with each of the p-coumaric acid (p-CoA) and gallic acid (GA) antimicrobial drugs by using the most available polymer common coatings (PVA) to expand the range of effective antibacterial drugs, as well as a mechanism for their synergistic effect via a simple method of preparation. Mechanistically, the average size of bare Fe3O4 NPs was ~15 nm, while RHL-coated Fe3O4@PVA@p-CoA/GA was about ~254 nm, with a drop in zeta potential from −18.7 mV to −34.3 mV, which helped increase stability. Our data show that RHL-Fe3O4@PVA@p-CoA/GA biosurfactant NPs can remarkably interfere with bacterial growth and significantly inhibited biofilm formation to more than 50% via downregulating IcaABCD and CsgBAC operons, which are responsible for slime layer formation and curli fimbriae production in S. aureus and E. coli, respectively. The novelty regarding the activity of RHL-Fe3O4@PVA@p-CoA/GA biosurfactant NPs reveals their potential effect as an alternative multitarget antivirulence candidate to minimize infection severity by inhibiting biofilm development. Therefore, they could be used in antibacterial coatings and wound dressings in the future.IMPORTANCE Antimicrobial resistance poses a great threat and challenge to humanity. Therefore, the search for alternative ways to target and eliminate microbes from plant, animal, and marine microorganisms is one of the world’s concerns today. Furthermore, the extraordinary capacity of S. aureus and E. coli to resist standard antibacterial drugs is the dilemma of all currently used remedies. Methicillin-resistant S. aureus (MRSA) and vancomycin-resistant S. aureus (VRSA) have become widespread, leading to no remedies being able to treat these threatening pathogens. The most widely recognized serotypes that cause severe foodborne illness are E. coli O157:H7, O26:H11, and O78:H10, and they display increasing antimicrobial resistance rates. Therefore, there is an urgent need for an effective therapy that has dual action to inhibit biofilm formation and decrease bacterial growth. In this study, the synthesized RHL-Fe3O4@PVA@p-CoA/GA biosurfactant NPs have interesting properties, making them excellent candidates for targeted drug delivery by inhibiting bacterial growth and downregulating biofilm-associated IcaABCD and CsgBAC gene loci.
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高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Xiangxiang Sun,Yumei Yu,Zhenyu Wang,Kumayl Hassan Akhtar,Ahmed S.M. Saleh,Wenhao Li,Dequan Zhang
Effects of braising duration on volatile organic compounds (VOCs) and lipids in chicken were investigated. Aroma profiles identified by an electronic nose were effective in differentiating braising stages. During braising process, a total of 25 key VOCs were detected in braised chicken, and sample braised for 210 min exhibited the highest level of key VOCs. Additionally, a gas chromatography mass spectrometry fingerprint was established to evaluate the distribution of VOCs throughout the braising process. Partial least square discriminant analysis indicated that 2-heptanone, 3-methyl-2-butanone, octanal, nonanal, butanal, (E)-2-pentenal, 1-octen-3-ol, 1-hexanol, pentanal, hexanal, and 1-pentanol significantly affected flavor characteristics of braised chicken. Furthermore, 88 differential lipids were screened, and glycerolipids metabolic was found to be main metabolic pathway during braising process. Triglycerides (TG) and phosphatidyl ethanolamine (PE), such as TG (16:0/18:1/18:2), TG (18:0/18:1/18:2), TG (18:1/18:2/18:3), TG (18:1/18:1/18:2), PE (O-18:2/18:2), PE(O-18:2/18:1), and TG (16:0/16:1/18:2), played a vital role in the generation of VOCs.
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DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Yasir Abbas,Sajjad Ali,Majid Basharat,H.M. Adeel Sharif,Muhammad Ahsan Iqbal,Mohamed Bououdina,Haoliang Wang,Niaz Ali Khan
The sustainable recovery of gold from electronic waste (E-waste) remains a significant challenge due to lack of multiple redox active-sites in the existing adsorbents, their high cost, and poor stability in extreme acidic conditions. Herein, we rationally designed the skeleton engineering of poly(organo)phosphazene via substitution reaction of hexachlorocyclotriphosphazene (HCCP) with p-phenylenediamine (DA), 1,4-benzenedithiol (DT), and 4-aminothiophenol (AT), producing poly(p-phenylenediamine-co-cylotriphosphazene) (PDA) with N-redox active-sites, poly(1,4-benzenedithiol-co-cylotriphosphazene) (PDT) containing S-redox active-sites, and poly(4-aminothiophenol-co-cylotriphosphazene) (PAT) holding dual N-S-redox active-sites. The PDA, PDT, and PAT in the N-bromosuccinimide/pyridine (NBS/Py) leaching solution demonstrated extraction efficiency of 83.7 % Au 3+ , 45.5 % Cu 2+, 44 % Ni 2+ , and 83.6 % Au 3+ , 47.4 % Cu 2+ , 48 % Ni 2+ , and 83.1 % Au 3+ , 48.5 % Cu 2+ , 48.8 % Ni 2+ . While, in the aqua regia, these adsorbents showed extraction efficiency of 70.0 % Au 3+ , 14.0 % Cu 2+ , 13.8 % Ni 2+ , and 92.3 % Au 3+ , 6.4 % Cu 2+ , 9.8 % Ni 2+ , and 95.6 % Au 3+ , 13.0 % Cu 2+ , 10.3 % Ni 2+ . In particular, the PAT with the dual N-S-redox active sites achieved maximal Au 3+ extraction efficiency (99.2 %), which is 1.05 and 1.04 times higher than PDA and PDT within 15 min, superb uptakes of 2000 mg/g, excellent selectivity (99.9 %) in a mixture of eight metal ions (Al, Co, Cu, K, Mg, Na, Ni, Mn), 100 % removal capacity and high stability even in pure aqua regia leaching solution. Gold ions adsorbed on these adsorbents, spontaneously reduced, experienced nucleation, and grew into single crystalline nanoplates along (111) planes as well as nanolayers composed of Au-single-atoms and Au-nanoclusters. The density function theory (DFT) calculations and variation in binding energies of N and S indicated that due to the synergy of dual N-S-redox active-sites, the PAT showed enhanced performances and excellent selectivity. For commercial applications, the as-synthesized PDA, PDT, and PAT were highly stable in pure aqua regia leaching solution, showing precisely selective and rapid gold extraction (70 %, 92 %, 96 %, respectively) than N- NBS/Py leaching systems (83 %) with traces of other competing metals. This study proves that skeleton-modulated poly(organo)phosphazene greatly facilitates gold extraction for industrial-scale applications.
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高影响因子
DRUG DELIVERY
·
2017
·
IF 6.402
Huiyun Zhang,Wenqian Xu,Emmanuel Omari-Siaw,Yingkun Liu,Baoding Chen,Deyu Chen,Jiangnan Yu,Ximing Xu
Periplocymarin (PPM), a cardiac glycoside, has a narrow therapeutic index, poor tumor selectivity and severe cardiovascular toxicity which hinder its wide clinical applications in cancer treatment. Herein, we report novel redox-responsive prodrug-nanoparticles (MPSSV-NPs) self-assembled by co-nanoprecipitation of PPM-vitamin E conjugate and a PEG derivative of linoleate (mPEG2000-LA) in water. It was found that the characteristics of PPM-vitamin E nanoparticles (PSSV-NPs) were improved through co-nanoprecipitation with increased percentages of mPEG2000-LA. Moreover, the MPSSV-NPs were optimized according to the in vitro release and cytotoxicity study. Furthermore, the optimized MPSSV-NPs dramatically enhanced the circulation time and tumor distribution of PSSV-NPs after single intravenous injection. The in vivo studies in malignant H22-bearing mice revealed that MPSSV-NPs could effectively suppress tumor growth without causing obvious systemic toxicity. Altogether, these results suggested that MPSSV-NPs could offer a safe, multifunctional and viable nanoplatform for cardiac glycosides in cancer treatment.
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DOI
PubMed
高影响因子
Food Bioscience
·
2025
·
IF 5.9
Fei Teng,Xixi Wu,Yue Liu,Lijia Li,Mengjie Geng
Emulsion gel is widely used in the delivery of functional components because of its network structure and mechanical properties. In this study, to improve the structural and functional properties of emulsion gels and achieve efficient encapsulation and release of functional components, transglutaminase (TGase) and genipin were employed to crosslink pH-shifting/ultrasonication-modified soybean lipophilic protein (SLP), enabling the simultaneous encapsulation of vitamin E (VE, oil phase) and quercetin (QU, aqueous phase), thereby developing a co-encapsulated emulsion gel system. The impacts of combined modification (pH-ultrasonication) and enzymatic/chemical crosslinking on gel properties were systematically investigated through gel strength analysis, rheological characterization, intermolecular interaction profiling, microstructural observation, and in vitro digestion evaluation. Results demonstrated that pH-shifting combined with ultrasonication exposed more hydrophobic groups in SLP, thereby enhancing intermolecular hydrophobic interactions. Microstructural analysis revealed that TGase and genipin synergistically strengthened network crosslinking density, forming a more compact gel network structure under modified conditions. Furthermore, TGase/genipin crosslinking significantly improved structural characteristics and rheological performance (e.g., water-holding capacity increased by 14.97 %, hardness and viscosity improved), while concurrently enhancing encapsulation efficiencies of both VE and QU. In vitro digestion test confirmed that the maximum release rate reached 97.43 ± 1.50 % (VE) and 90.39 ± 2.43 % (QU) ( p < 0.05). These findings elucidated the potential for constructing emulsion gels via combined modification and crosslinking strategies, providing theoretical guidance for co-encapsulation systems in functional factors delivery applications.
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DOI
高影响因子
Innovative Food Science & Emerging Technologies
·
2025
·
IF 6.8
Jingjing Li,Zehong Fang,Xueqing Huang,Yuhang Cai,Boxin Jiang,Giancarlo Cravotto,Zhengya Dong,Zhilin Wu
Ultrasonic emulsification frequently serves to prepare nanoemulsions containing active ingredients, including Vitamin E (VE), which can be degraded by Reactive Oxygen Species (ROS) generated during conventional ultrasonication. To address this issue, VE nanoemulsions were continuously prepared using a novel temperature-controlled ultrasonic tube-in-tube reactor (USTIT). Effects of formulation and process parameters on physical and chemical stability were systematically investigated. The mixing surfactant of Tween-80/Span-80 produced smaller, more stable, and uniform droplets (135 nm), remaining stable for 100 days. As the aqueous to oil (A/O) ratio increased, the droplet sizes decreased significantly, and the VE retention increased. With antioxidant Ascorbyl Palmitate (AP), the VE half-life reached 862 days at an A/O ratio of 15/1, significantly enhancing VE stability at room temperature. Moreover, VE degradation kinetics changed from first-order to second-order, leading to more persistent protection of VE by AP. In addition, lower ultrasonic power, higher flow rate, and higher temperature significantly reduced ROS yield and VE degradation. Based on the accelerating degradation kinetics of VE during a 60 °C holding period, VE nanoemulsions with AP, prepared at 45 W of ultrasonic power, 3 mL/min of flow rate, and at 50 °C, exhibited a long VE half-life of 205 h due to uniform ultrasonic energy distribution, and controllable residence time and temperature. In contrast, the VE half-life only reached 92.6 h by a conventional ultrasonic probe and 170.1 h by an ultrasonic bath. Therefore, the USTIT process provides a foundation for the continuous, stable preparation of high-value lipid-soluble bioactive nanoemulsions.
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高影响因子
Food Chemistry-X
·
2026
·
IF 9.2
Jianyu Yuan,Miaomiao Tian,Wenting Chen,Macao Zhuo,Guang Li,Yue Wang
The low water solubility and poor thermal stability of Vitamin E (VE) limit its application in food processing. This study investigated the effects of the synergistic combination of electron beam irradiation (EBI) at doses of 0, 5, and 10 KGy and ultra-high pressure (UHP) at 100, 300, and 500 MPa on the preparation of potato starch-VE complexes. EBI pretreatment significantly reduced the degree of branching and enhanced complexation, antioxidant activity, and resistant starch (RS) content in a dose-dependent manner, whereas increasing UHP pressure progressively diminished these attributes. The combination of 10 KGy EBI with 100 MPa UHP yielded the highest RS content (67.24%) and DPPH scavenging activity (85.02%). XRD analysis indicated no V-type crystalline formation, suggesting weak but sustained complexation. The parameter-dependent effects of EBI and UHP offers a promising approach to produce starch-VE complexes with improved antioxidant capacity and digestion resistance, benefiting functional food development for diabetics.
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DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2026
·
IF 7.1
Qun-Yan Xiong,Hui-Wen Gu,Qing-Qing Luo,Chao Wang,Yan-Qing Fu,Xiao-Li Yin
A machine learning-driven strategy for optimal feature selection is proposed. • W1W and W1C sensors serve as key variables for aroma type classification. • 131 volatile compounds were identified in Liupao tea. • 14 aroma type differentiating compounds were identified. • 4 key variables yielded 100% accurate aroma recognition models. This study aimed to differentiate between the stale-aroma and betelnut-aroma types of Liupao tea by analyzing their volatile profiles. An integrated methodology was employed, combining electronic nose (E-nose) technology with headspace gas chromatography-ion mobility spectrometry (HS-GC-IMS). The E-nose data effectively differentiated between the two aroma types in both dry leaves and tea infusions. HS-GC-IMS enabled identification of 131 volatile compounds. Key discriminative features were selected through machine learning by integrating the variable importance in projection (VIP) from orthogonal partial least squares discriminant analysis (OPLS-DA) with Shapley additive explanations (SHAP) values from extreme gradient boosting (XGBoost). This process identified two critical E-nose sensor channels and two key volatile compounds. Utilizing these four optimal variables, three classification models were developed: principal component analysis-linear discriminant analysis (PCA-LDA), partial least squares discriminant analysis (PLS-DA), and support vector machine (SVM). All models demonstrated a perfect classification accuracy of 100% on both the training and internal test datasets. The PCA-LDA and PLS-DA models maintained 100% accuracy on the independent test set, whereas the SVM model achieved a classification accuracy of 80% on the same set. These results reveal the volatile characteristics of Liupao tea and offer valuable insights for quality assessment and targeted flavor modulation.
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高影响因子
Environmental Chemistry and Ecotoxicology
·
2026
·
IF 12.3
Yuxian Liu,Yutan Cai,Dongmei Li,Huajun Meng,Yufeng Lai,Jingtai Xu,Yongyin Lan,Xuan Zeng,Bo Zhang,Zhipeng Cheng,Tao Zhang,Jingchuan Xue
Modern e-waste dismantling workshops showed substantially elevated BTH and BTR levels in indoor dust. • 2-OH-BTH predominated in dust, reaching 8100 ng/g in modern e-waste dismantling workshops. • 2-OH-BTH and 1-H-BTR dominated urinary profiles, with 1.8-fold higher concentrations in e-waste workers than rural residents. • BTH EDIs via dust ingestion were ∼ 24-fold higher in workers, yet dust contributed ≤2.33% of total exposure. • Significant intra- and inter-class correlations were observed for BTHs and BTRs in both dust and urine. Benzothiazoles (BTHs) and benzotriazoles (BTRs) are high production volume chemicals widely incorporated into electronic products, yet their environmental occurrence and potential occupational exposure during e-waste recycling remain poorly characterized. In this study, four BTHs and three BTRs were quantified in 113 dust samples collected from a modern e-waste recycling facility and 15 household dust samples, as well as in urine samples from 121 e-waste dismantling workers and 20 rural residents. The median Σ(BTHs+BTRs) in modern e-waste (e.g., cell phone) dismantling workshop dust (3000 ng/g) was approximately 9–10 times higher than that observed in traditional e-waste (e.g., refrigerator) dismantling workshop (350 ng/g), e-waste storage areas (260 ng/g) and suburb residential homes (300 ng/g). These findings indicate substantial accumulation of BTHs and BTRs in indoor dust associated with modern e-waste dismantling activities. 2-Hydroxybenzothiazole (2-OH-BTH) was the predominant congener in e-waste associated dust, accounting for more than 84–95% of Σ(BTHs+BTRs) across different microenvironments, compared with 73% in reference residential dust, suggesting relatively modest differences in congener profiles between recycling and residential environments. In urine, 2-OH-BTH and 1H-BTR were the predominant detected congeners, accounting for 83% and 67% of Σ(BTHs+BTRs) in workers and rural residents, respectively. Their median urinary concentrations in workers were 4.3 and 1.8 ng/mL, respectively, representing 1.8-fold higher levels than those in rural residents (2.4 and 1.0 ng/mL). Significant positive correlations were observed among BTHs, among BTRs, and between the two chemical classes in both dust and urine, suggesting shared or related exposure sources. The median estimated daily intake of BTHs through dust ingestion among workers was 2.60 ng/kg bw/day, approximately 24-fold higher than that estimated for rural residents (0.11 ng/kg bw/day), indicating elevated occupational exposure potential. Nevertheless, dust ingestion accounted for no more than 2.33% of the total BTH exposure estimated from urinary biomonitoring, suggesting that other exposure pathways may make greater contributions to overall internal exposure.
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高影响因子
FOOD RESEARCH INTERNATIONAL
·
2026
·
IF 8.8
Qi Zhao,Yicheng Zhou,Cong Wang,Mingxuan Li,Shan Zhu,Chang Xue,De Ji,Yuwen Qin,Chengxi Jiang
Gastrodia elata Bl. ( GE ), a widely utilized edible and functional material, exhibits notable quality variations due to distinct growing conditions across different geographical origins, making reliable methods for origin authentication and quality evaluation essential. This study integrated machine vision, flash GC e-nose and HPLC to analyze GE samples from Anhui (AH), Shaanxi (SN), and Yunnan (YN) provinces in China. Results showed comparable color characteristics among geographical origins, but distinct textural differences: SN samples displayed more complex textures with higher surface roughness, YN samples showed richer textural details but less distinct edges and lower periodicity, while AH samples exhibited smoother surfaces. Eleven flavor compounds were identified as potential flavor markers. Although no significant differences were found in the total content of six marker components, three linear machine learning models (PLS, SVM, and LDA) established based on multidimensional features achieved 100% classification accuracy in training and prediction sets, providing a reliable approach for GE geographical origin traceability.
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高影响因子
ACS Applied Nano Materials
·
2023
·
IF 5.9
Zhiying Li,Fuhai Guo,Sheng Tian,Ru Liu,Dongchun Yang,Xin Wang,Haiqing Hu,Yilong Wang,Jian Zhao
For its significant technological interest, a systematic investigation on mechanical and thermal conduction properties of natural rubber reinforced by functionalized hexagonal boron nitride nanosheets is highly desirable. We herein first demonstrate a unique synthesis of hydroxyl-functionalized boron nitride nanosheets (OH-BNNSs) via a simple one-pot reduction reaction. This involves negative charging by treating BNNSs with lithium in liquid ammonia and subsequent bubbling of oxygen into dispersion of BNNS salts. Subsequently, a series of functionalized boron nitride nanosheets/natural rubber (NR) composites were fabricated by latex mixing and co-coagulation. Co-coagulating a stable aqueous suspension of OH-BNNSs with natural rubber latex afforded a weblike morphology consisting of platelet networks between the latex particles. Compared to pure rubber, the tensile strength and storage modulus at 25 °C for OH-BNNSs/NR (5 wt %) are increased by 466% and 1 order of magnitude, respectively. Benefiting from the unique structure, the composite at 5 wt % shows a 40% improvement in thermal conductivity relative to pure rubber. This latex-based method provides a facile and effective strategy to design and fabricate advanced functional BNNS-based polymeric nanocomposites with a segregated filler network morphology and considerable property enhancements in direct competition with graphene sheets. The composites could be potentially used in heat dissipative tires or conveyor belts.
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高影响因子
Food Chemistry-X
·
2023
·
IF 6.1
Yuting Rong,Jialing Xie,Haibo Yuan,Lilei Wang,Fuqiao Liu,Yuliang Deng,Yongwen Jiang,Yanqin Yang
Storage time is one of the important factors affecting the aroma quality of Pu-erh tea. In this study, the dynamic changes of volatile profiles of Pu-erh teas stored for different years were investigated by combining gas chromatography electronic nose (GC-E-Nose), gas chromatography-mass spectrometry (GC–MS), and gas chromatography-ion mobility spectrometry (GC-IMS). GC-E-Nose combined with partial least squares-discriminant analysis (PLS-DA) realized the rapid discrimination of Pu-erh tea with different storage time (R 2 Y = 0.992, Q 2 = 0.968). There were 43 and 91 volatile compounds identified by GC–MS and GC-IMS, respectively. A satisfactory discrimination (R 2 Y = 0.991, and Q 2 = 0.966) was achieved by using PLS-DA based on the volatile fingerprints of GC-IMS. Moreover, according to the multivariate analysis of VIP > 1.2 and univariate analysis of p < 0.05, 9 volatile components such as linalool and (E)-2-hexenal were selected as key variables to distinguish Pu-erh teas with different storage years. The results provide theoretical support for the quality control of Pu-erh tea.
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PubMed
高影响因子
危险品化学品经营许可证（带存储）