Chem & Bio Engineering
·
2026
·
IF 8.4
He Qin,Cheng Shuai,Yue Jianqing,Zong Shu,Li Congyu,Qi Xuerong,Liu Lipei,Zhang Zhidan,Sun Jun,Cheng Jian,Lu Xiaoyun,Jiang Huifeng
3 ′ -O-amino-deoxyribonucleoside
triphosphates (3 ′ -ONH 2 -dNTPs) are crucial reversible
terminators for template-independent
DNA synthesis. Owing to the structural complexity of purine bases,
here we propose a chemo-enzymatic process for the synthesis of purine-type
3 ′ -ONH 2 -dNTPs. First, the aminoalkoxyl group was
introduced into commercially available dA and dG via a chemical catalytic
process, affording yields of 24.9% and 27.3%, respectively. Subsequently,
to establish a green and scalable enzymatic cascade for phosphorylation
of the substrates, we engineered two key phosphorylation enzymes (Dm-dNK
and MrPPK2), improving the rate-limiting monophosphorylation of 3 ′ -ONH 2 -dG from 26% to 97.8% and achieving 59.9% conversion to the
corresponding triphosphate. Lastly, the produced nucleotides exhibit
performance equivalent to commercial counterparts in terminal deoxynucleotidyl
transferase (TdT)-mediated DNA synthesis, supporting the construction
of 120-nt strands with ∼99.7% stepwise accuracy. This work
establishes an efficient and generalizable catalytic platform for
producing modified dNTPs, advancing the practical application of enzymatic
DNA synthesis technologies.
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Nature Communications
·
2026
·
IF 18.1
Zhao Zihan,He Zhen,Gu Hongming,Zhou Andong,Wang Yibing,Liang Zhaoyi,Geng Jie,Xu Xuejiao,Wang Menghui,Li Chunyao,Fan Yuying,Cheng Hairong,Sun Lin,Mayo Kevin H.,Raz Avraham,Tai Guihua,Zhou Yifa
The mechanism of unconventional protein secretion remains an unresolved issue. Here, we describe an unconventional protein secretion pathway for galectin-3 that is mediated by phase separation and condensation. Using four lysosomal damage models, we observed a rapid, pronounced release of galectin-3 in large, non-exosomal particles. This secretion is driven by glycoprotein-induced galectin-3 phase separation and is independent of pyroptosis and secretory autophagy. During phase separation, the S-face of galectin-3 carbohydrate recognition domain binds glycoproteins that triggers galectin-3 N-terminal tail release and condensation. These condensates then recruit ALG-2 via the exposed N-terminal tail. ALG-2 directs the condensates to the endoplasmic reticulum–late endosome interface. After translocation into late endosomes, galectin-3 condensates are secreted into the extracellular milieu by SNARE-dependent vesicular transport. This mechanism of exporting phase-separated protein condensates may serve as a clean-up response to membrane damage.
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Science China-Chemistry
·
2026
·
IF 9.8
Dai Huan,Yang Mingyu,Yang Hongkun,Liu Haiquan,Ke Zunjian,He Dong,Xiao Xiangheng
Electrochemical nitrate reduction (NO 3 RR) represents an environmentally sound pathway for converting nitrate ions (NO
− 3
) to ammonia in wastewater. However, the mechanism governing NO
− 3
hydrogenation under altered liquid-phase conditions remains poorly understood. This study enhances the reactivity of CeO 2 toward nitrate hydrogenation by loading it with transition metals and further modifying the composition of alkali metal cations in the electrolyte. This approach regulates the continuity of both interfacial water and hydrogen-bond networks, facilitating NO
− 3
-proton coupling and thereby boosting NO 3 RR reactivity. Furthermore, the incorporation of diverse surfactants into the electrolyte confirms that alterations in the liquid-phase environment influence interfacial water distribution, ultimately improving NO 3 RR reactivity. This study deepens our understanding of interfacial water’s role in NO 3 RR, providing a theoretical basis for developing more efficient electrocatalysts.
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CELLULAR SIGNALLING
·
2026
·
IF 4.7
Guangyuan Li,Mingyu Peng,Mengxue Li,Ruoyu Hu,Chunxiao Gao,Yamin Xing,Ting Zhang,Jixuan Xu,Miaoran Fu,Wulong Liang,Pengya Feng,Hongqiao Zhang,Zisen Zhang,Pengyuan Zheng,Xiufeng Chu
Pyroptosis is a form of programmed cell death mediated by gasdermin proteins, with GSDMD and GSDME being the most extensively studied. Inflammatory caspases-1 or caspases-4/5/11 cleave GSDMD and release its pore-forming fragment GSDMD-NT, whereas the apoptotic caspase-3, cleaves GSDME and releases its pore-forming fragment GSDME-NT. In this study, we observed that caspase-3 is activated during GSDMD-mediated pyroptosis. Interestingly, downregulation of caspase-3 activity either through RNAi or caspase-3 inhibitor significantly increased cell death. Furthermore, we found that caspase-3 physically interacts with GSDMD-NT and cleaves it at a site distinct from those targeted by caspase-1 or caspases-4/5/11. This alternative cleavage generates a non-functional fragment of GSDMD-NT, thereby disrupting its integrity. Consequently, we demonstrate that caspase-3 activation serves as a negative feedback mechanism to regulate the intensity of GSDMD-NT-mediated pyroptosis.
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JOURNAL OF ALLOYS AND COMPOUNDS
·
2015
·
IF 2.999
Jian Liu,Xiulan Duan,Yang Zhang,Ziqing Li,Fapeng Yu,Huaidong Jiang
Rb 2 Ti 1.95 Yb 0.05 (PO 4 ) 3 crystals with langbeinite-type structure were grown by high-temperature solution method. The chemical composition and electronic structure were analyzed using inductive coupled plasma atomic emission spectroscopy (ICP-AES) and X-ray photoelectron spectroscopy (XPS). The deconvolution of Ti 2p 3/2 photoemission line reveals two peaks at 459.3 eV and 458.1 eV, indicating titanium existence in two valence states: Ti 4+ (459.3 eV) and Ti 3+ (458.1 eV). The reduced BE difference ΔBE (O 1s-Ti 2p 3/2 ) shows stronger ionicity of Ti–O bonds in Rb 2 Ti 1.95 Yb 0.05 (PO 4 ) 3 compared with RbTiOPO 4 . Additionally, UV absorption, specific heat, and second harmonic generation (SHG) efficiency were studied. The UV absorption edge of the Rb 2 Ti 1.95 Yb 0.05 (PO 4 ) 3 crystal was observed at 368 nm. The SHG intensity of Rb 2 Ti 1.95 Yb 0.05 (PO 4 ) 3 is 5–6 times greater than that of KDP crystal when the particle size is between 38 and 74 μm.
研究领域:
材料科学
·
物理学
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Materials Futures
·
2022
·Vol. 1
·期 4
·045201
·
IF 0
Kangqiao Cheng,Wei Xie,Shuo Zou,Huanpeng Bu,Jin-Ke Bao,Zengwei Zhu,Hanjie Guo,Chao Cao,Yongkang Luo
Rhodium-containing compounds offer a fertile playground to explore novel materials with superconductivity (SC) and other fantastic electronic correlation effects. A new ternary rhodium-antimonide La2Rh_{3+\delta}Sb4 (\delta\approx 1/8) has been synthesized by a Bi-flux method. It crystallizes in the orthorhombic Pr2Ir3Sb4-like structure, with the space group Pnma (No. 62). The crystalline structure appears as stacking the two-dimensional RhSb4- and RhSb5-polyhedra networks along b axis, and the La atoms embed in the cavities of these networks. Band structure calculations confirm it as a multi-band metal with a van-Hove singularity like feature at the Fermi level, whose density of states are mainly of Rh-4d and Sb-5p characters. The calculations also imply that the redundant Rh acts as charge dopant. SC is observed in this material with onset transition at T_c^\mathrm{on}\approx 0.8 K. Ultra-low temperature magnetic susceptibility and specific heat measurements suggest that it is an s-wave type-II superconductor. Our work may also imply that the broad Ln_2Tm_{3+\delta}Sb4 (Ln = rare earth, Tm = Rh, Ir) family may host new material bases where new superconductors, quantum magnetism and other electronic correlation effects could be found.
研究领域:
凝聚态物理学
·
材料科学
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JOURNAL OF ORGANIC CHEMISTRY
·
2017
·
IF 4.849
Jian Cheng,Jun Sun,Jiekuan Yan,Song Yang,Pengcheng Zheng,Zhichao Jin,Yonggui Robin Chi
The metal-free catalytic functionalization of aromatic sp2-carbons and benzylic sp3-carbons remains challenging. Here we report a carbene-catalyzed functionalization of the 3-methyl sp3-carbon attached to 2-formyl-indoles. The reaction proceeds through an NHC-bound o-quinodimethane as the key intermediate generated from 2-formyl-3-methylindoles under oxidative conditions. Reactive ketones are found to be effective substrates to produce substituted hydropyrano[3,4-b]indoles in good to excellent yields.
研究领域:
有机化学
·
合成化学
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Acta Pharmaceutica Sinica B
·
2020
·
IF 7.097
Meng Yuan,Xiaobing Chen,Yitang Sun,Li Jiang,Zhongni Xia,Kaixiong Ye,Hong Jiang,Bo Yang,Meidan Ying,Ji Cao,Qiaojun He
The membrane protein claudin-3 (CLDN3) is critical for the formation and maintenance of tight junction and its high expression has been implicated in dictating malignant progression in various cancers. However, the post-translational modification of CLDN3 and its biological function remains poorly understood. Here, we report that CLDN3 is positively correlated with ovarian cancer progression both in vitro and in vivo. Of interest, CLDN3 undergoes S -palmitoylation on three juxtamembrane cysteine residues, which contribute to the accurate plasma membrane localization and protein stability of CLDN3 . Moreover, the deprivation of S -palmitoylation in CLDN3 significantly abolishes its tumorigenic promotion effect in ovarian cancer cells. By utilizing the co-immunoprecipitation assay, we further identify ZDHHC12 as a CLDN3-targating palmitoyltransferase from 23 ZDHHC family proteins. Furthermore, the knockdown of ZDHHC12 also significantly inhibits CLDN3 accurate membrane localization, protein stability and ovarian cancer cells tumorigenesis . Thus, our work reveals S -palmitoylation as a novel regulatory mechanism that modulates CLDN3 function, which implies that targeting ZDHHC12-mediated CLDN3 S -palmitoylation might be a potential strategy for ovarian cancer therapy.
研究领域:
癌症生物学
·
细胞生物学
·
分子生物学
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FULLERENES NANOTUBES AND CARBON NANOSTRUCTURES
·
2018
·
IF 1.011
Min-Zun Ji,Feng-Chao Guo,Pu Zhang,Zhi-Xin Guo
The mechanochemical reaction of C60 and Bi(NO3)3·5H2O was performed under ball-milling condition. The product was well characterized by spectroscopic methods such as IR, Raman, UV-vis, 13C NMR, as well as elemental analysis method. The spectroscopic investigation indicates that some hydroxyl and nitro groups are attached to the C60 and no profound structural changes occur for the C60 cage in the product. The elemental analysis gives an average composition of C60(NO2)2(OH)21 for the product. A possible mechanism is suggested for the reaction.
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LANGMUIR
·
2020
·
IF 3.557
Kun He,Cong Shen,Yanqing Zhu,Xiaoli Chen,Zhuoneng Bi,Thandapani Marimuthu,Gang Xu,Xueqing Xu
Perovskite nanomaterials have been fascinating for commercial applications and fundamental research owing to their excellent optical properties and satisfactory processability. They are expected to be alternative downconversion materials in phosphor-converted LEDs for lighting or display technology. However, owing to their low formation energy and large specific surface area, perovskite nanomaterials are sensitive to environmental stress like humidity, heat, etc. In this paper, cubic CsPbI3 quantum dots (QDs) with improved stability are synthesized using (3-aminopropyl)triethoxysilane (APTES). These luminescent CsPbI3 QDs passivated by APTES not only show excellent stability when stored in hexane but also possess outstanding steadiness for lattice structure when prepared as a thin film in open air. They do not decompose immediately in the water. Such excellent stability is attributed to the hindrance from hydrolysis of APTES, which forms an analogous core–shell structure to protect the “core” CsPbI3 QDs. Furthermore, an additional iodine source is added to enhance their emissionm and CsPbI3QDs with a PLQY of 84% are synthesized.
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BIOCHEMICAL ENGINEERING JOURNAL
·
2022
·
IF 4.446
Shan Yuan,Miaomiao Jin,Chao Xu,Wencheng Yin,Mingsha Zhao,Mo Xian,Wei Liu
3-(Aminomethyl) pyridine (3-AMP) is a versatile compound for the synthesis of pharmaceuticals, agricultural chemicals and numerous polymers. Efficient and mild biocatalytic synthesis is often an excellent supplement to traditional chemical synthesis methods. However, there has been no report of biocatalytic 3-AMP synthesis. In this paper, 3-AMP was synthesized for the first time by using biocatalysts co-expressing carboxylic acid reductase from Neurospora crassa (ncCAR) and ω-transaminase from Ochrobactrum anthropi (OATA) in one pot. In addition, the metabolic pathway was optimized by knocking out the transcriptional dual regulator arcA , which resulted in a 90% increase in the yield of 3-AMP. Furthermore, efficient self-sufficient whole-cell bioconversion was established by optimization, achieving 93% yield and a 40.6 mM (4.4 g/L) titer of 3-AMP without additional carbon sources. This whole-cell bioconversion system was also suitable for the high-efficiency biocatalytic synthesis of other aromatic or heterocyclic primary amines, such as benzylamine, 2-furfurylamine, 4-fluorobenzylamine, 2,4-difluorobenzylamine and 2-(aminomethyl) pyridine. Being entirely biocatalytic, our one-pot procedure provides considerable advantages in terms of economic, environmental and safety impacts over reported chemical methods.
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BIOSENSORS & BIOELECTRONICS
·
2021
·
IF 10.618
Lihang Zhu,Shuang Li,Wenxin Liu,Jing Chen,Qiwen Yu,Zhen Zhang,Yi Li,Jun Liu,Xing Chen
As an oxidase stress biomarker, 3-nitrotyrosine is closely associated with many cardiovascular diseases. Thus, early diagnosis and real time detection of 3-nitrotyrosine at bedside are highly important. Herein, we developed a handheld electrochemiluminescence (ECL) analysis device, which integrates printed circuit board (PCB) for electrical stimulation and smartphone for optical signals readout. Fast and accurate determination of 3-nitrotyrosine was achieved with Antibody/Ru(dcpy) 3 2+ @AuNPs/MoS 2 modified Au electrode (Ab/Ru@AuNPs/MoS 2 ) for ECL analysis. The linear range of 3-nitrotyrosine detection was from 10 −8 mol/L to 10 −6 mol/L with a detection limit of 8.4 × 10 −9 mol/L. In addition, an Android application was developed to realize real time analysis of ECL emissions and results readout for detection. To confirm the usage of the device, spiked serum with different concentrations was tested and the results indicated the practical reliability and stability of this device. The operating procedure for ECL analysis in this device is extremely easy and electrical stimulation was adjustable from 0 V to 5 V for general ECL systems. Thus, we believe this handheld device for ECL analysis has extensive prospects for application in Point-of-care testing and health caring.
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