ACS Synthetic Biology
·
2025
·
IF 3.9
Zhen Zong,Lianghua Xie,Jiaqi Fu,Zhongyang Liu,Wen-Wen Zhou,Wei Chen
Cyanidin-3-O-galactoside (C3Ga), a natural pigment, has various beneficial biological activities and is widely used as a food colorant. However, traditional plant extraction methods are time-consuming and unsustainable. Rapid and sustainable synthesis of C3Ga by engineered microorganisms offers a promising alternative to traditional plant-based methods and deserves to be explored. In this study, the bioproduction of C3Ga by Escherichia coli was achieved for the first time. The biosynthetic pathway of C3Ga from (+)-catechin was constructed by introducing anthocyanidin synthase (ANS) and UDP-galactose:cyanidin galactosyltransferase. Some strategies, including enhancement of the UDP-galactose biosynthesis pathway, identification of efficient ANS, overexpression of the C3Ga transporter, and modulation of multigene expression, were subsequently used to drive the metabolic flux toward C3Ga production. Next, the two-stage process for C3Ga production was optimized to mitigate limitations for further metabolic engineering. Combined with the knockout of β-phosphoglucomutase (ycjU), a newly identified competitive pathway for UDP-galactose, the production of C3Ga finally reached 217.9 mg/L. The strategies used in this study could be applied to the biosynthesis of other anthocyanins and galactosylated natural products.
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INORGANIC CHEMISTRY
·
2026
·
IF 4.7
Hangwei Jia,Yaqi Jin,Junwei Feng,Bailin Chen,Feng Yu,Xueling Hou
Guanidine units have garnered significant attention in the study of ultraviolet (UV) nonlinear optical (NLO) materials due to their excellent microscopic optical properties, making them ideal systems for developing novel optical devices. A novel guanidinium-templated UV NLO crystal, [C(NH2)3]SbCl4 (GSC), was successfully synthesized via an aqueous solution method. GSC crystallizes in the orthorhombic chiral space group P212121, featuring a seesaw shaped [SbCl4]− anion. Remarkably, GSC exhibits a moderate second harmonic generation (SHG) response of 1.1 × KDP at 1064 nm and a large refractive index difference of 0.2 at 546 nm. This NLO effect originates from the synergistic interaction between the [C(NH2)3]+ unit and the stereochemically active [SbCl4]− unit. This work provides a candidate for the guanidine family of NLO materials.
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Analytical Methods
·
2026
·
IF 2.6
Bingyu Fu,Yanru Shao,Zhixiong Zheng,Shengxin Wen,Yingli Guo,Qi Xu,Hualin Yang
In this study, a simple and sensitive dual-mode biosensor was designed based on the color of 2,6-dichloroindiphenol (DCIP) and the fluorescence of calcein. The target alkaline phosphatase (ALP) can catalyze the dephosphorylation of L-ascorbic acid-2-phosphate (AAP) to form ascorbic acid (AA) and phosphate ions. The reducing properties of AA can reduce the DCIP from blue to colorless. Meanwhile, phosphate ions can chelate with europium ions (Eu 3+ ) to restore the fluorescence of Eu 3+ -Calcein. In colorimetric mode, the linear detection range for ALP was 1.56-50 U/L with a limit of detection (LOD) of 0.27 U/L. In fluorescence mode, the linear detection range was 0.78-25 U/L and LOD was 0.23 U/L. Besides, the selectivity of this dual-mode for ALP was very excellent and the recovery rates in serum samples were also satisfactory.
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ACS Sustainable Chemistry & Engineering
·
2018
·
IF 6.14
Liqiao Zhong,Yong Lu,Haixia Li,Zhanliang Tao,Jun Chen
Aqueous rechargeable sodium-ion batteries (ARSIBs) are promising candidates for large-scale energy storage applications due to their high safety, low cost, and environmental friendliness. However, appropriate anode materials with high capacity for ARSIBs are limited, and their cycling stability is generally unsatisfactory. Here we report high-performance ARSIBs with polyacrylamide hydrogel as electrolyte and alloxazine (ALO) encapsulated in CMK-3 as anode. The hydrogel with solid content of 60% could effectively mitigate the dissolution issue of sodiated ALO because its cross-linked structure is helpful to reserve H2O. The ALO/CMK-3 anode based on a two-electron transfer reaction could deliver a high capacity of 160 mA h g–1. The introduction of CMK-3 could improve the electrical conductivity of ALO and further reduce the dissolution of sodiated ALO because of its high conductivity and nanochannel structure. The full ARSIBs exhibit an energy density of 50 W h kg–1 (based on the total mass of active electrode materials) with good capacity retention of 90% after 100 cycles at 2 C and high rate capability of 146 mA h g–1 at 10 C (1 C = 250 mA g–1). This work paves the way to construct high-performance ARSIBs with high-capacity organic anode and hydrogel electrolyte.
研究领域:
能源材料
·
电化学
·
电池技术
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Journal of Cleaner Production
·
2022
·
IF 11.072
Yufei Zhang,Junhua Yun,Lei Zhou,Guoyang Zhang,Mei Zhao,Hossain M. Zabed,Xianghui Qi
To achieve carbon neutrality and carbon peaking goals, biomanufacturing of high-value chemicals from sustainable biorefinery has emerged as an attractive alternative to traditional petrochemical-based processes. 3-Hydroxypropionic acid (3-HP) is one of the top value-added building-block chemicals with diverse applications, which has increased its demand for large-scale industrial production. Nevertheless, sustainable biosynthesis of 3-HP is limited by the lack of robust strains and the need for expensive fermentation medium components. Herein, efforts were made to overcome these limitations through metabolic engineering of Escherichia coli and process optimization. First, the functional 3-HP biosynthetic pathway was recruited and optimized in E. coli . Next, the endogenous glycerol facilitator (GlpF) was overexpressed to enhance glycerol uptake, and the expression level of aldehyde dehydrogenase (YdcW) was optimized for speeding up 3-HP biosynthesis. Subsequently, global regulator engineering further improved 3-HP production. Additionally, corn steep liquor (CSL) was used to develop a cheap fermentation medium. Finally, co-fermentation of crude glycerol with glucose and acetic acid using the final strain produced 67.62 g/L 3-HP with a yield and a productivity of 0.86 mol/mol glycerol and 1.41 g/L/h, respectively.
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CERAMICS INTERNATIONAL
·
2022
·
IF 5.532
Huiru Wang,Jingyi Liu,Sorour Sadeghzade,Runsheng Hou,Hongyan Yuan
Among piezo composites, 0–3 type ceramic/polymer-based composites have balanced properties and flexible manufacturing processes, allowing them to tackle challenges in wearable electronics and sensing technology. However, due to a misfit between the dielectric coefficients of the ceramic particles and polymer matrix , poling 0–3 composites is difficult. Therefore, in this study, we develop a novel poling method that combines the merits of normal corona poling and oil-based poling methods to activate a lead zirconium titanate (PZT)/Polyethylene glycol diacrylate (PEGDA) composite. The influence of various parameters (voltage, temperature, poling duration, and time of ageing) on the piezoelectric and dielectric properties of the PZT/polymer composite were evaluated. For the 0–3 PZT/PEGDA composites (67 wt%), the optimal poling parameters were found to be 60 min poling duration, 25 °C, and 24 kV. The d 33 (32 pC/N) obtained by the proposed poling method is about 3 times that obtained by the normal corona poling method (11 pC/N). This modified poling method was shown to be more effective than the conventional method for a range of filler contents (50 wt%, 67 wt%, and 75 wt%). It was also found that the d 33 value of the PZT/PEGDA composite increased over time and was attributed to the increase of the composite dielectric constant after poling. Finally, sensing elements were assembled from the poled samples and evaluated, which shows the potential for detecting tiny signals in sensing and soft robotics.
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FUEL
·
2022
·
IF 8.035
Chengrui Wang,Yanhong Fang,Zhenghao Wang,Mujun Long,Dengfu Chen,Huamei Duan,Yandong Li,Guoquan Zhang
Partially oxidizing methane into syngas via a two-step chemical looping scheme was a promising option for methane transformation. Ce 2 (SO 4 ) 3 was selected to explore the performance and mechanism of methane chemical looping partial oxidation (CLPOM) to syngas. Thermodynamic and kinetic analysis forecast the Ce 2 (SO 4 ) 3 as a promising candidate for CLPOM. Compared with literature, Ce 2 (SO 4 ) 3 not only had an outstanding syngas selectivity, but also an appropriate CH 4 conversion and molar ratio, and CH 4 conversion could reach 90.5 % of thermodynamic result. As revealed via characterization, Ce 2 (SO 4 ) 3 (1 0 2) surface had the highest peak intensity and the migration of lattice oxygen played an important role in CLPOM. Some of them were tend to agglomeration and these small particles were less than 5 μm. Density functional theory calculations demonstrated that CH 4 series dehydrogenation products tended to adsorb at O site and CH 3 → CH 2 + H was the rate-determining step. The reaction of surface lattice oxygen atoms and migration of the internal lattice oxygen atoms could inhibit carbon deposition, promote CH 4 conversion and syngas generation in experiment. Compared with NiO, Ce 2 (SO 4 ) 3 had a lower energy barrier of lattice oxygen migration, which could increase the reaction rate.
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INORGANIC CHEMISTRY
·
2022
·
IF 5.436
Dan Zhao,Wen Liu,Ya-Nan Li,Rui-Juan Zhang,Li-Na Zhang
A modulated structure with symmetrical characteristic higher than three-dimensional is a fascinating crystallographic type, and it is randomly encountered by researchers. Herein, we prepared 0.1 mm level single crystals of Na3La(VO4)2 and determined its structure to be a (3 + 1)-dimensional modulated structure using the X-ray diffraction analysis method for single crystals. Its super space group was determined to be Pca21(0β0)000. On the other hand, we introduced Tb3+ into the Na3La(VO4)2 host lattice to fabricate phosphors Na3La1-x(VO4)2:xTb3+ and studied their photoluminescence properties. Interestingly, for the strong absorption of the Na3La(VO4)2 host lattice in the range of 200–400 nm, the traditional 330–385 nm light is unable to efficiently excite Tb3+ ions in the Na3La(VO4)2 host to generate luminescence of Tb3+. Instead, Na3La1–x(VO4)2:xTb3+ is suitable to be excited by 487 nm to generate emitting light at 543, 584, and 622 nm, due to Tb3+ characteristic 4f → 4f transitions of 5D4 → 7FJ (J = 5, 4, 3). Hence, the Tb3+-doped Na3La(VO4)2 phosphors have potential applications for white-light-emitting diodes.
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CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY
·
2022
·Vol. 100
·期 7
·
IF 2.245
Yanzhen Han,Yan Zhou,Liyuan Zhou,Xiaoyan Jia,Xiangjun Yu,Xiaohong An,Zhe Shi
Chemotherapy is one of the most important strategies in the treatment of cancer; however, chemoresistance restricts the effect of chemotherapy. Growing reports suggest that chloride channel-3 (ClC-3) is involved in regulating the sensitivity of multiple chemotherapeutic agents in the chemotherapy of various tumours, while its role in the chemotherapy of cholangiocarcinoma (CCA) is still poorly understood. Herein, we observed that ClC-3 was highly expressed in CCA chemoresistant tissues and CCA cisplatin-resistant cells QBC939/DDP, and the sensitivities of QBC939 and QBC939/DDP cells to cisplatin were all increased after inhibition of ClC-3. Further mechanism exploration revealed that ClC-3 knockdown reduced the level of autophagy. Furthermore, in both QBC939 and QBC939/DDP cells, the autophagy agonist rapamycin eliminated the increased cisplatin sensitivity of ClC-3 knockdown without affecting ClC-3 expression. Collectively, all the findings demonstrate that ClC-3 knockdown increases cisplatin-induced cell death in CCA cells though inhibiting autophagy, regardless of the occurrence of cisplatin resistance. In addition, our results also suggest that targeted inhibition of ClC-3 may be a potential strategy for chemosensitization in CCA chemotherapy.
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CRYSTENGCOMM
·
2022
·Vol. 24
·期 16
·2966-2975
·
IF 3.756
Shuang-Yin Zhu,Dan Zhao,Shao-Jie Dai,Rui-Juan Zhang,Lin-Ying Shi
For Mn-activated phosphors, the luminescent performance is strongly dependent on the oxidation state of Mn. In this paper, a series of red phosphors NaMg(PO3)3:xMn2+ (NMP:xMn2+) were synthesized by high temperature solid-state reaction in the open atmosphere. The single oxidation state of Mn2+ was confirmed by X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), and photoluminescence spectroscopy (PL). We suspected that a self-reduction process occurred to prevent the oxidation of Mn2+. The doped Mn2+ ions occupy the strong crystal field environment of the Mg site (octahedral point), forming a red luminescence center with a peak position centered at 630 nm and a full width at half maximum (FWHM) of 80 nm. The thermal stability and quenching luminescence are explained by the temperature dependence of the PL spectrum. The results show that the NaMg(PO3)3:Mn2+ red phosphor has potential applications in LED backlight display areas.
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Processes
·
2022
·Vol. 10
·期 7
·1302
·
IF 3.352
Hailun Ren,Weihong Li
3-hydroxybutyraldehyde (3-HBA) is mainly employed to synthesize 1,3-BDO (1,3-butanediol), which is one of the most important components in cosmetics moisturizers. In this study, a series of composite oxide catalysts were prepared by bringing alkaline earth metal Ca and rare earth metal La to the composite oxide MgO/Al2O3, which were made through the co-precipitation method. These catalysts were applied in the synthesis of 3-HBA through acetaldehyde (AcH) condensation. The structure, texture, and acidic properties of these catalysts were characterized using various characterization methods, and the effects of catalyst composition, reaction temperature, reaction pressure, and residence time on the conversion of AcH were investigated as well. The results showed that the introduction of Ca and La weakened the acidic property and enhanced the basic property, which favored the AcH condensation to synthesize 3-HBA. At a temperature of 20 °C, pressure of 200 kPa, and residence time of 70 min, 0.5%La-2.3%Ca-2MgO/Al2O3exhibited a better catalytic activity, and the conversion of AcH reached 95.89%. The selectivity and yield of 3-HBA were 92.08% and 88.30%, respectively. The stability test indicated that the high AcH conversion could be maintained for 5 h.Keywords:composite oxides;acetaldehyde;condensation catalyst;3-hydroxybutyraldehyde
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JOURNAL OF THE EUROPEAN CERAMIC SOCIETY
·
2022
·
IF 6.364
Ling Pan,Ling He,Zibo Niu,Peng Xiao,Wei Zhou,Yang Li
In this work, Yb 4 Hf 3 O 12 ceramic, a typical rare-earth hafnate, was exposed to water vapor with Al(OH) 3 impurities at 1400 ℃, and microstructural evolution and corrosion mechanisms were investigated. The long-term corrosion can be divided into initial hydration and gasification of Hf 4+ and steady-state corrosion after the formation of Al 5 Yb 3 O 12 . In the initial stage, Hf 4+ and the surrounding O 2- tend to be hydrated and then volatilized in the form of Hf(OH) 4 , causing a metastable state of Yb 4 Hf 3 O 12 on the surface. Besides, the preferential corrosion of small grains, grain boundary, and specific crystal plane on the surface and sintered and densified grains result in varied corrosion behavior from the early linear law to the later parabolic law. In the steady-state corrosion stage, due to the consumption of Hf 4+ , more vacancies promote bonding and diffusion of Al 3+ to form corrosion product (Al 5 Yb 3 O 12 ), and during this stage, regrowth and shedding of the corrosion layer reach a dynamic balance.
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