JOURNAL OF APPLIED POLYMER SCIENCE
·
2024
·
IF 2.7
Yonghua Zhai,Lili Wu,Jie Gao,Jingsheng Zhou,Longhai Du,Xiaoda Fu,Yang Gao,Tinglin Zhang,Mengya Zhang,Weihong Lu,Yan Wu
Smart gene delivery vectors are gaining increasing attention in gene therapy, owing to their low cytotoxicity and intrinsic responsiveness. Our previously fabricated novel cationic brush polymer, comprising C Se bonds and tertiary amine EGIn- g -PDMAEMA, shows potential for gene transfection. In this study, its high efficiency for siRNA/pDNA transfection and low cytotoxicity in reactive oxygen species (ROS)-rich microenvironments is substantiated in vitro. Its superior binding capacity with siRNA/pDNA is confirmed by agarose gel electrophoresis assay. The threshold weight ratios for siRNA/pDNA migration delay are 15 and 3 (polymer-to-nucleic acid, w/w), respectively. Fluorescence microscopy and ribonucleotide reductase regulatory subunit M2 gene silencing essay verify the biodegradability and responsive control release of nucleic acids under hydrogen peroxide stimulation in Huh-7 cells. Compared with the gold standard, polyethylenimine 25 kDa, the target polymer displays superior transfection efficiency in ROS-rich tumor cells under serum-free conditions. Furthermore, the vector–nucleic acid complexes exhibit over 90% cell viability at a high concentration of 12 μg mL −1 and good colloidal stability in phosphate-buffered saline (PBS) and 10% fetal bovine serum-PBS for 24 h. The efficient control release and expression of nucleic acids in ROS environments and reduced cytotoxicity highlight the superiority of EGIn- g -PDMAEMA as a gene delivery platform for tumor gene therapy.
研究领域:
生物医学工程
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基因治疗
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材料科学
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分子生物学
✓ 已验证
DOI
高影响因子
EUROPEAN JOURNAL OF INORGANIC CHEMISTRY
·
2024
·Vol. 27
·期 24
·e202400204
·
IF 2.2
Haibo Li,Chen Su,Qianqian Wang,Jing Yang,Jialin Zhu,Hua Tong,Ning Zhao,Wei Liu,Gangfeng Ouyang
Graphical A neutral copper iodide hybrid with unique Cu 3 I 3 trimeric inorganic functional unit is reported. The hybrid owns a one-dimensional extended polymeric structure in the form of Cu 3 I 3 ( L ) 2 ( L =5,6,7,8-tetrahydroquinoxaline) and has an infrequent far-red/near-infrared emission. The luminescence mechanism is studied to be the combination of metal-to-ligand and halide-to-ligand charge transfer by the results of decay lifetime measurement and calculation of density of states. In this paper, a neutral copper iodide hybrid with unique Cu 3 I 3 trimeric inorganic functional unit is successfully fabricated using 5,6,7,8-tetrahydroquinoxaline ( L ) with large steric-hindrance group as the organic ligand. The hybrid owns a one-dimensional (1D) extended polymeric structure in the form of Cu 3 I 3 ( L ) 2 . As a CuI-based hybrid, 1D-Cu 3 I 3 ( L ) 2 has an infrequent far-red/near-infrared emission, exhibiting the potential application prospects in the field of display and night vision. Moreover, the luminescence mechanism is carefully studied by decay lifetime measurement and calculation of density of states, which is verified to be the combination of metal-to-ligand charge transfer and halide-to-ligand charge transfer.
研究领域:
材料科学
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无机化学
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发光材料
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光电子学
✓ 已验证
DOI
高影响因子
Materials Today Physics
·
2024
·
IF 10
Jingru Wang,Jiaxin Yang,Yanyan Li,Yang Li,Rui Xu,Feng Song
The widespread application of optical encryption materials with difficult imitation, multicolor-tunable emission and simple operation presents a promising strategy for secure communication and authentication of information. Herein, Eu-MOF grafted with coumarin 7 is successfully constructed through self-assembly process and a prismatic three-dimensional hyperbranched structure is formed. At room temperature, it can be excited to by ultraviolet light significantly different green and red light emission. With the temperature rising to 363 K, under the excitation of 360 nm, a continuous and programmable color transformation occurs, resulting in a fitted line with a fitting degree of 0.99745. In order to maintain robust reliability, regular alterations in accordance with temperature variations are assessed through temperature sensitivity, and the absolute sensitivity and relative sensitivity can reach up to 6.168 % K −1 and 4.387 % K −1 . Based on the abundant emission modes, we develop an asymmetric information security authentication program to simulate encrypted information communication, which provides reliable guidance on visualized information storage and encryption.
研究领域:
光学材料
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密码学
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材料科学
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化学
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信息安全
✓ 已验证
DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2021
·
IF 4.952
Yin Zheng,Li Li,Zekun Jin,Peipei An,Shang-Tian Yang,Yongtao Fei,Gongliang Liu
The use of Schleiferilactobacillus ( S. ) harbinensis M1 as a starter culture, has been shown to improve the organoleptic quality of fermented soymilk, but its contribution to nutrient transformation and health-related functionality remains unclear. This study characterized the biotransformation of carbohydrates, isoflavones , proteins and free amino acids, as well as the antioxidant and antiproliferative activities of soymilk fermented by S. harbinensis M1. After fermentation, soybean carbohydrates were mainly converted into lactic acid (76.68 mmol/L) and acetic acid (8.19 mmol/L), isoflavone glucosides (daidzin and genistin) were partially converted to aglycones (daidzein and genistein). Soybean proteins were extensively hydrolyzed into oligopeptides and free amino acids, and the soluble peptide fraction (451-189 Da) increased from 4.6% to 86%. Whole-genome sequencing of S. harbinensis M1 revealed a 3,592,195 bp circular chromosome with 53.02% (G + C) content and 3375 coding genes. Metabolic prediction revealed the enzymes required for carbohydrate/pyruvate metabolism and isoflavone biotransformation. The whole proteolytic system was predicted, including peptide transport system and 66 genes encoding proteinases/peptidases. S. harbinensis M1 significantly ( p < 0.05) improved the antioxidant activity and antiproliferative effects of soymilk against HepG2/MCF-7 cells. These findings suggest that S. harbinensis M1 can be used as a proteolytic starter-culture to make functional fermented foods .
研究领域:
食品科学
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微生物学
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基因组学
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营养学
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生物化学
✓ 已验证
DOI
高影响因子
Materials
·
2022
·Vol. 15
·期 22
·7930
·
IF 3.748
Ze-Kun Zhang,Shi-Peng Ding,Ze Ye,Ding-Li Xia,Jun-Ting Xu
In this work, the advanced all solid-state block copolymer electrolytes (SBCPEs) for lithium-ion batteries with double conductive phases, poly(ethylene oxide)-b-poly(trimethyl-N-((2-(dimethylamino)ethyl methacrylate)-7-propyl)-ammonium bis(trifluoromethanesulfonyl) imide) (PEO-b-PDM-dTFSI)/LiTFSI, were fabricated, in which the charged PDM-dTFSI block contained double quaternary ammonium cations and the PEO block was doped with LiTFSI. The disordered (DIS) and ordered lamellae (LAM) phase structures were achieved by adjusting the composition of the block copolymer and the doping ratior. In addition, the presence of the hard PDM-dTFSI block and the formation of the LAM phase structure resulted in a good mechanical strength of the solid PEO-b-PDM-dTFSI/LiTFSI electrolyte, and it could maintain a high level of 104Pa at 100 °C, which was around 10,000 times stronger than that of the PEO/LiTFSI electrolyte. Based on the good mechanical and electrochemical properties, the PEO-b-PDM-dTFSI/LiTFSI SBCPE exhibited excellent long-term galvanostatic cycle performance, indicating the strong ability to suppress lithium dendrites.
研究领域:
材料科学
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能源科学
✓ 已验证
DOI
高影响因子
Biochemistry and Cell Biology
·
2024
·
IF 2.4
Xiang Chen,Yuan Yao,Guotong Gong,Tianji He,Chenjun Ma,Jingsong Yu
Prostate cancer (PCa) is a complex disease with diverse molecular alterations. The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor that exhibits pleiotropic roles in PCa, and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) is a potent ligand for AhR. While targeting ferroptosis is an innovative PCa therapeutic strategy, the impact of AhR on this process remains unclear. This study aimed to investigate the influence of AhR on lipid peroxidation and ferroptosis. Results showed that TCDD activated AhR, as evidenced by increased CYP1A1 expression, leading to reduced cell viability. TCDD caused mitochondria shrinkage, decreased the GSH/GSSG ratio, and elevated the MDA levels and lipid peroxidation. Interestingly, AhR knockdown reversed these effects, similar to the action of ferroptosis inhibitors. Mechanistically, TCDD suppressed nuclear receptor subfamily 4 group A member 1 (NR4A1) expression, in part due to AhR activation. This suppression subsequently led to a reduction in the expression of the NR4A1 downstream target stearoyl-CoA desaturase 1 (SCD1). NR4A1 overexpression counteracted the effects of TCDD. In vivo, TCDD activated AhR, downregulated NR4A1 and SCD1 expression, induced mitochondria shrinkage, and increased the MDA and 4-hydroxynonenal (4-HNE) levels. In summary, TCDD promotes ferroptosis in androgen-dependent PCa via inhibiting the NR4A1/SCD1 axis, in part dependent on AhR activation.
研究领域:
肿瘤学
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分子生物学
·
药理学
✓ 已验证
DOI
PubMed
高影响因子
Phenomics
·
2022
·Vol. 2
·期 2
·119-129
·
IF 0
Huang Sha,Yang Chuanwei,Li Lin
For shade-intolerant plants, a reduction in the red/far-red (R:FR) light ratio signals the close proximity of competitors and triggers shade-avoidance syndrome (SAS). Auxin, brassinosteroid, gibberellin and some transcriptional regulators have been reported to regulate shade-induced hypocotyl elongation. However, little is understood regarding the coordination of these multiple regulatory pathways. Here, combining time-lapse growth rates and transcriptomic data, we demonstrate that auxin and brassinosteroid affect two phases of shade-induced rapid growth, whereas gibberellin mainly contributes to the second rapid growth phase. PHYTOCHROME-INTERACTING FACTOR 7 (PIF7) acts earlier than other PIFs. PIF4 and PIF5 modulate the second rapid growth phase. LONG HYPOCOTYL IN FAR-RED 1 (HFR1) and PIF3-LIKE 1 (PIL1) modulate two rapid growth phases. Our results reveal that hormonal and transcriptional regulatory programs act together to coordinate dynamic hypocotyl changes in an immediate response to a shade signal and provide a novel understanding of growth kinetics in a changing environment.
研究领域:
植物生物学
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分子生物学
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植物生理学
✓ 已验证
DOI
DYES AND PIGMENTS
·
2023
·
IF 4.5
Xiumin Liu,Hengwei Zhang,Fengting Hu,Yi Wang,Xu Fei,Jing Tian
In this paper, an efficient and novel 1,3-bis(2-pyridylimino)-4,7-dihydroxyisoinndole scaffold-based two-site fluorescent probe 1,3-bis(2-pyridylimino)-4,7-phenylene diacrylate isoindole (BPI-AC) for the sensing of cysteine (Cys) has been designed and synthesized. We have studied the chemical structure and its fluorescent properties for the novel fluorescent probe with double recognition group by means of theoretical calculations and experimental measurements, which were carried out using density functional theory (DFT) and time-dependent density functional theory (TDDFT) calculations and experiments on organic chemical synthesis. Firstly, geometries have been recorded for the fluorescent probe. Theoretical research parameters including spectroscopic parameters, molecular orbitals , reaction sites and energy barrier Δ were obtained by fitting the electron density in a multiparticle system. Additionally, the spectral characteristics , selectivity and anti-interference performance of the probe were systematically investigated and verified by in vitro experiments. The experimental results showed that by using this probe, it was possible to quantitatively detect Cys in 1% dimethyl sulfoxide-containing water solution (pH 7.4). Super large Stokes shift (265 nm) endowed the probe low background signal. Finally, according to our theoretical calculations and the available experimental data, the probe BPI-AC with dual recognition sites could distinguish the biothiols with similar structure through series recognition reaction, and the Stokes shift was large, realizing the efficient detection of Cys specificity.
研究领域:
分析化学
·
理论化学
·
生物化学
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2015
·
IF 3.65
Lejiao Jia,Zhenyu Li,Jingyi Shen,Dandan Zheng,Xiaona Tian,Hejian Guo,Ping Chang
The objective of the study is to fabricate multifunctional mesoporous silica nanoparticles for achieving co-delivery of conventional antitumor drug paclitaxel (PTX) and the multidrug resistance reversal agent tetrandrine (TET) expecting to overcome multidrug resistance of MCF-7/ADR cells. The nanoparticles were facile to prepare by self-assemble in situ drug loading approach. Namely, PTX and TET were solubilized in the cetyltrimethylammonium bromide (CTAB) micelles and simultaneously silica resources hydrolyze and condense to form nanoparticles. The obtained nanoparticles, denoted as PTX/TET-CTAB@MSN, exhibited pH-responsive release property with more easily released in the weak acidic environment. Studies on cellular uptake of nanoparticles demonstrated TET could markedly increase intracellular accumulation of nanoparticles. Furthermore, the PTX/TET-CTAB@MSN suppressed tumor cells growth more efficiently than only delivery of PTX (PTX-CTAB@MSN) or the free PTX. Moreover, the nanoparticle loading drugs with a PTX/TET molar ratio of 4.4:1 completely reversed the resistance of MCF-7/ADR cells to PTX and the resistance reversion index was 72.3. Mechanism research showed that both TET and CTAB could arrest MCF-7/ADR cells at G1 phase; and besides PTX arrested cells at G2 phase. This nanocarrier might have important potential in clinical implications for co-delivery of multiple drugs to overcome MDR.
研究领域:
纳米技术
·
药物科学
·
癌症研究
·
药物递送系统
·
生物医学工程
✓ 已验证
DOI
PubMed
高影响因子
BIOORGANIC & MEDICINAL CHEMISTRY
·
2023
·
IF 3.5
Guiying Wu,Hui Zhong,Ying Wang,Li Chen,Jianbo Sun
A number of NO-releasing quinoline derivatives have been designed and synthesized by introducing NO donor to quinoline carboxylic acid fragment. The anti-proliferation of all target compounds was evaluated against human cancer cell lines (HCT-116, MCF-7, and A549), MCF-7/ADR and normal cell (MCF-10A). Most compounds showed cytotoxic activity on cancer cells and drug-resistant cells with IC 50 values in the range of 0.62–5.51 μM. Importantly, these compounds showed low toxicity to normal cells (4.21–34.08 μM). Further mechanism studies showed that the most potent compound 9 could release high concentration of NO and inhibit the activity of topoisomerase I. In addition, 9 regulated apoptosis-related proteins, generated ROS and blocked MCF-7 cells in G2/M phase to induce cell apoptosis. Furthermore, the P-gp-mediated transport was also influenced by 9 . And 9 could significantly inhibit the growth of tumor in vivo without observable organ-related toxicities. Overall, as a novel NO-releasing quinoline derivative, 9 was worthy for further in-depth study.
研究领域:
肿瘤学
·
药理学
·
分子生物学
·
细胞生物学
✓ 已验证
DOI
PubMed
高影响因子
TOXICOLOGY AND APPLIED PHARMACOLOGY
·
2020
·
IF 3.347
Xiaolei Wang,Kaiyan Lou,Xiaodong Song,Huijuan Ma,Xinyi Zhou,Huan Xu,Wei Wang
Mebendazole (MBZ) is a tubulin-suppressive antihelmintic agent with low toxicity, which has been repurposed to treat different types of tumors. Chemoresistance is quite common in refractory or relapsed T cell acute lymphoblastic leukemia (T-ALL), which leads to dismal chances of recovery. In this study, MBZ was found to suppress the proliferation and reduce the viability of T-ALL cell line, CCRF-CEM, and its chemoresistant derivative, CEM/C1, at nanomolar concentrations. The inhibitive effects were found to be dose-dependent and not to be affected by the chemoresistance of CEM/C1 cells. Cell cycle arrest, caspase 3/7 activation and tubulin disruption were found in the MBZ-treated T-ALL cells. Notch1 signaling, which is often aberrantly activated in T-ALL cells, was showed to be suppressed by MBZ treatments. MBZ administration in murine T-ALL models also suppressed the growth of CEM/C1 cells, indicating that MBZ may be developed as a therapeutic agent for chemoresistant T-ALLs. The mRNA levels of the Notch1 and Hes1 were also confirmed to be suppressed by MBZ in vivo, which was consistent with the in vitro observations. This study demonstrated, for the first time, that MBZ could inhibit chemoresistant T-ALL cells both in vitro and in vivo , and the Notch1 signaling pathway was suppressed by MBZ treatment.
研究领域:
肿瘤学
·
药理学
·
细胞生物学
✓ 已验证
DOI
PubMed
高影响因子
Biomaterials Science
·
2018
·Vol. 7
·期 3
·1011-1027
·
IF 5.831
Xiaoqun Shi,Yang Wang,Haiyan Sun,Yujuan Chen,Xingzhen Zhang,Jiangkang Xu,Guangxi Zhai
Graphene-based nanomaterials (GBNMs) have great potential in drug delivery and photothermal therapy owing to their unique physicochemical properties. However, inferior water solubility and biocompatibility related issues greatly restricted their further applications. Herein, to rectify the obstructive problems, we prepared uniform and smaller sized graphene oxide (GO) nanosheets (∼85 nm) via a modified Hummers’ method, which exhibited significantly improved hemocompatibility compared to random large sized GO nanosheets prepared by a common method. Then, we grafted unfractionated heparin (UFH) onto reduced graphene oxide (rGO) covalently using adipic acid dihydrazide (ADH) as a linker to fabricate biocompatible nanocomposites for the cellular delivery of curcumin (Cur). The novel nanocomposites showed quite a small size of 42 nm in average lateral dimension and exhibited a significantly stronger photothermal effect than GO nanosheets. Besides, in vitro cell experiments verified that the potential anticancer efficacy of Cur-loaded vehicles and cytotoxicity of rGO-UFH/Cur against MCF-7 and A549 cells could be further enhanced under 808 nm irradiation, suggesting the possibility of combinational chemotherapy and photothermal therapy. Moreover, consistent with the in vitro sustained drug release performance, an in vivo pharmacokinetics study also indicated that the retention time of Cur could be significantly prolonged when loaded on rGO-UFH nanocomposites than in free Cur solution. Notably, we firstly discussed the interaction between rGO and Cur, and demonstrated the meliorative biocompatibility of uniform rGO compared to GRO via a molecular dynamics simulation (MD) study. Thus, the in vitro, in vivo and computational study demonstrated that the small sized rGO-UFH nanocomposites had wide application prospects as drug delivery vehicles.
研究领域:
纳米技术
·
药学
·
生物医学工程
·
材料科学
·
肿瘤学
·
计算生物学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）