Journal of Fungi
·
2024
·Vol. 10
·期 2
·151
·
IF 4.2
Xianran Zhu,Shaoying Zhang,Youwei Yu,Shengwang Li,Chao Yang,Yuan Chang
Alternaria alternatais the main pathogenic fungus of postharvest black spots in fruits and vegetables. This study aimed to explore the antifungal activity of methionine onA. alternatain vitro and to reveal related antifungal mechanisms through a metabolomics analysis. The results showed that the inhibitory effects of L-methionine (Met) treatment on mycelium growth, spore germination, and the germ tube elongation ofA. alternatawere enhanced with an increase in the Met concentration, but the inhibitory effects decreased when the Met concentration was higher than 50 mmolL−1. The results of propidium iodide staining and scanning electron microscopy showed that the Met treatment damaged the plasma membrane integrity of theA. alternataspores and caused an irreversible deformation of mycelium. In addition, after the Met treatment, the leakage of electrolytes, nucleic acid, and proteins in theA. alternatacells was significantly higher than that in the control group, indicating that the Met treatment increased the permeability of the cell membranes. Eighty-one different metabolites, divided into seven categories, were identified through the metabolomics analysis, including forty-three downregulated metabolites and thirty-eight upregulated metabolites. Among them, these differential metabolites were mainly involved in amino acid synthesis and metabolism, the pentose phosphate pathway, and the TCA cycle. Therefore, the antifungal effect of the Met treatment onA. alternatawas mainly to damage the integrity of the cell membranes, make nucleic acid and protein contents leak, and affect the TCA cycle, carbohydrate metabolism, amino acid synthesis metabolism, and the metabolic pathways associated with cell membrane biosynthesis. Thus, the growth and development ofA. alternatawere inhibited. The research enriched the investigation of the effect of the antifungal mechanism of Met treatment onA. alternataand provided a theoretical basis for the application of Met to prevent and treat postharvest black spots in fruits and vegetables.
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高影响因子
Bioactive Materials
·
2024
·
IF 18
Tong Zhao,Xingdan Liu,Zhuangzhuang Chu,Jing Zhao,Dongya Jiang,Xiaohua Dong,Ziyi Lu,Kelvin W.K. Yeung,Xuanyong Liu,Liping Ouyang
Infection and poor tissue repair are the key causes of percutaneous implantation failure. However, there is a lack of effective strategies to cope with due to its high requirements of sterilization, soft tissue healing, and osseointegration. In this work, l -arginine (L-Arg) was loaded onto a sulfonated polyetheretherketone (PEEK) surface to solve this issue. Under the infection condition, nitric oxide (NO) and reactive oxygen species (ROS) are produced through catalyzing L-Arg by inducible nitric oxide synthase (iNOS) and thus play a role in bacteria sterilization. Under the tissue repair condition, L-Arg is catalyzed to ornithine by Arginase-1 (Arg-1), which promotes the proliferation and collagen secretion of L929 and rBMSCs. Notably, L-Arg loading samples could polarize macrophages to M1 and M2 in infection and tissue repair conditions, respectively. The results in vivo show that the L-Arg loading samples could enhance infected soft tissue sealing and bone regeneration. In summary, L-Arg loading sulfonated PEEK could polarize macrophage through metabolic reprogramming, providing multi-functions of antibacterial abilities, soft tissue repair, and bone regeneration, which gives a new idea to design percutaneous implantation materials.
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LANGMUIR
·
2025
·
IF 3.9
Xuan Chen,Zheng Chen,Lang Wu,Feifei Hu,Xin Zhou,Qian Lin,Hongyan Pan,Yongyong Shi,Donghai Jiang
Using an l-arginine (LA)-functionalized Pd–Ni catalyst, hydrogen peroxide (H2O2) was directly synthesized via a self-designed microreactor at ambient temperature and pressure. The yield of H2O2 achieved 574.31g kgcat–1 h–1, with the concentration of H2O2 in the solution reaching 4.05 wt % per hour. After five cycles and online activation, the LA functionalized Pd–Ni catalyst maintained high H2O2 catalytic activity. The catalytic activity evaluation experiments, characterization results, and simulation studies demonstrated that Ni doping and LA-functionalization synergistically modulate the electronic structure of the active Pd component. This modulation increases the concentration of Pd2+ at the active sites, effectively inhibits the dissociation of the O–O bond in O2*, OOH*, and HOOH* species, thereby enhancing the catalytic performance for H2O2 production. This study proposes a systematic strategy for the design of Pd-based catalysts to directly synthesize highly efficient H2O2.
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高影响因子
Journal of Environmental Chemical Engineering
·
2023
·
IF 7.7
Chen Cai,Jiayin Chen,Wei Fu,Shuying Ning,Hao Zhou,Muhammad Kashif,Yaxin Su
The Ce-modified OMS-2 catalyst (Ce x -OMS-2) was synthesized by the hydrothermal synthesis method, after which its catalytic efficacy in the selective reduction of NO by CO was assessed. The experiments showed that Ce 0.08 -OMS-2 was capable of 100% NO conversion between 175 and 300 °C when 0.05%NO, 0.1%CO, 5%O 2 and N 2 were used as equilibrium gases, and the N 2 selectivity peaked at 93.2% at 175 °C. Additionally, Ce 0.08 -OMS-2 exhibits good sulfur resistance in the presence of 0.02% SO 2 and maintains more than 88% NO conversion at 175–275 °C. By using BET, XPS, H 2 -TPR, NH 3 -TPD, and In situ DRIFTS to characterize the catalysts, the effects of doping the active metal Ce on the physicochemical characteristics of OMS-2 catalysts were under investigation. In conclusion, the improvement of the low-temperature activity of the Ce x -OMS-2 catalyst is facilitated by lower agglomeration and crystallinity , larger specific surface area, smaller average pore size, enhanced surface oxygen vacancies, increased Mn 4+ concentration, and richer Brønsted acid. Reaction gas adsorbed species and intermediates on the catalyst surface were dynamically captured during In situ DRIFTS experiments. The CO-SCR reaction of the catalyst was found to follow the E-R mechanism below 200 ℃ and the L-H mechanism at 200–350 ℃.
研究领域:
环境科学
·
催化化学
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高影响因子
CHEMOSPHERE
·
2022
·
IF 8.943
Gufeng Li,Mingrong Lu,Shaoqing Li,Min Yang,Yuanling Zhang,Yanli Zhang,Hongbin Wang,Wenrong Yang
Mercury ion (Hg 2+ ) is a heavy metal pollutant that can affect the safety of water environment and endanger human health. A novel detection strategy (GNPs- L -Cys-Rh6G2) for Hg 2+ based on a fluorescence “OFF-ON” was proposed. Gold nanoparticles (GNPs) were assembled with l -cysteine ( L -Cys), which was used as a “bridge” to link with rhodamine 6G derivatives (Rh6G2). The fluorescence state transition of GNPs- L -Cys-Rh6G2 switching from “OFF”-“ON” was observed because Hg 2+ opened the spirolactam ring through a catalytic hydrolysis mechanism. The fluorescence signal of the GNPs- L -Cys-Rh6G2 system mixed with Hg 2+ in the concentration range of 10–100 μM was analyzed and determined with a limit of detection (LOD) of 2 μM ( S/N = 3). Moreover, the spiked Hg 2+ concentration in real water samples were successfully quantified by GNPs- L -Cys-Rh6G2, which was in line with the ideal average recovery rate and relative standard deviation. The proposed strategy exhibited high specificity, sensitivity and stability, providing a novel sensing platform for heavy metal ions detection in water environment.
研究领域:
环境科学
·
分析化学
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高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2021
·
IF 13.273
Lei Xing,Jieru Bao,Shiyu Zhou,Yulong Qiu,Hongji Sun,Shuai Gu,Jianguo Yu
Poor economics is the principal factor that hinders the commercialization of hydrometallurgy processing of spent lithium-ion batteries cathode materials. Here, ultra-fast leaching of spent cathode materials (97.72% Li and 97.25% Co leached under S/L ratio: 10 g/L, temperature: 363 K within 10 min) was achieved in 0.5 M hydrochloric acid and 0.5 M L -ascorbic acid system as part of reducing the costs of hydrometallurgy process. The correlations of both acids with the leaching ratios were studied for the first time with the Pearson correlation coefficient. The mixture of 0.5 M hydrochloric acid and 0.5 M L -ascorbic acid demonstrated both faster kinetics and higher leaching volume than those of aqua regia. Adopting quantum chemistry and thermodynamic calculations, four hypothetical mechanisms were proposed to interpret the reaction mechanisms. Synergy-coordination, with the lowest energy, was considered to be the most reasonable mechanism for the leaching of cathode materials. Three scenarios with different leaching systems and conditions were compared to identify the key parameters determining the economics of the leaching process.
研究领域:
环境科学
·
化学工程
·
材料科学
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高影响因子
CHEMISTRY-A EUROPEAN JOURNAL
·
2023
·Vol. 30
·期 10
·e202302936
·
IF 4.3
Feiyan Yuan,Jing Ding,Yiyang Sun,Jianhua Liang,Yunzi Luo,Yang Yu
Graphical The carbene transfer reaction was realized in the biosynthetic pathway. Using L-limonene as the precursor, L-carveol was biotransformed by P411-PFA to generate unnatural terpenoid derivatives with trifluoromethyl group. Protein engineering of cytochrome P450s has enabled these biocatalysts to promote a variety of abiotic reactions beyond nature‘s repertoire. Integrating such non-natural transformations with microbial biosynthetic pathways could allow sustainable enzymatic production of modified natural product derivatives. In particular, trifluoromethylation is a highly desirable modification in pharmaceutical research due to the positive effects of the trifluoromethyl group on drug potency, bioavailability, and metabolic stability. This study demonstrates the biosynthesis of non-natural trifluoromethyl-substituted cyclopropane derivatives of natural monoterpene scaffolds using an engineered cytochrome P450 variant, P411-PFA. P411-PFA successfully catalyzed the transfer of a trifluoromethyl carbene from 2-diazo-1,1,1-trifluoroethane to the terminal alkenes of several monoterpenes, including L-carveol, carvone, perilla alcohol, and perillartine, to generate the corresponding trifluoromethylated cyclopropane products. Furthermore, integration of this abiotic cyclopropanation reaction with a reconstructed metabolic pathway for L-carveol production in Escherichia coli enabled one-step biosynthesis of a trifluoromethylated L-carveol derivative from limonene precursor. Overall, amalgamating synthetic enzymatic chemistry with established metabolic pathways represents a promising approach to sustainably produce bioactive natural product analogs.
研究领域:
生物化学
·
合成生物学
·
药物化学
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高影响因子
ACS Applied Materials & Interfaces
·
2020
·
IF 8.758
Wenyu Zhang,Xiaojie Liu,Benlin He,Jingwei Zhu,Xueke Li,Kaixiang Shen,Haiyan Chen,Yanyan Duan,Qunwei Tang
The perovskite solar cells (PSCs) based on cesium lead bromide (CsPbBr3) with outstanding environmental stability and low preparation cost are regarded as one of the most promising photovoltaic devices for commercial applications. However, the performance of CsPbBr3 PSCs can be badly deteriorated by the intense charge recombination arising from the ionic defects at the grain boundaries of perovskite film. To cope with this issue, we adopt an amino acid of l-lysine with two amino and one carboxyl groups as a chemical additive to incorporate into perovskite film to simultaneously anchor the uncoordinated Pb2+ (Cs+) and halogen ion defects. Further, the grain size of CsPbBr3 perovskite is boosted from 688 to over 1000 nm after l-lysine incorporation as a result of the decreased nucleation rate and the sufficient growth of perovskite, which effectively reduce the grain boundaries for load defects. As expected, the optimized device achieves a best power conversion efficiency of 9.69% attributed to the remarkably reduced charge recombination and enhanced charge extraction arising from the efficient defects dual-passivation and enlarged grain size of perovskite film as well as the improved energy level alignment at the device interface after the introduction of l-lysine, which is elevated by 61.23% in comparison to 6.01% efficiency of the pristine one. Moreover, the unencapsulated device with l-lysine incorporation exhibits remarkable long-term stability in air with 80% RH at 25 °C and 0% RH at 80 °C as well as under continuous illumination conditions. This work provides an effective multifunctional additive for imperfection passivation and grain size enlargement of perovskite to build PSCs with high efficiency and stability.
研究领域:
材料科学
·
能源技术
·
光伏器件
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高影响因子
Scientific Reports
·
2017
·Vol. 7
·期 1
·1-9
·
IF 4.259
Liu Qingju,Chen Li,Zhang Zhikun,Du Bibai,Xiao Yating,Yang Kunhao,Gong Lingling,Wu Li,Li Xiangjun,He Yujian
D-amino acid oxidases (DAAO) are stereospecific enzymes which are generally almost inactive towards L-enantiomer in neutral solution when L-, D-amino acids are supplied as substrates. In this paper, the D-amino acid oxidase can catalytic oxidize L-amino acids by modulating pH of aqueous solution. With L-Pro as substrate, the catalytic rate ( k
cat ) and the affinity ( K
m ) of DAAO were 6.71 s −1 and 33 mM at pH 8.0, respectively, suggesting that optimal pH condition enhanced the activity of DAAO towards L-Pro. Similar results were obtained when L-Ala (pH 9.8), L-Arg (pH 6.5), L-Phe (pH 9.0), L-Thr (pH 9.4), and L-Val (pH 8.5) were catalyzed by DAAO at various pH values. The racemization of the L-amino acids was not found by capillary electrophoresis analysis during oxidation, and quantification analysis of L-amino acids before and after catalytic reaction was performed, which confirmed that the modulation of enantioselectivity of DAAO resulted from the oxidation of L-amino acids rather than D-amino acids by changing pH. A mechanistic model was proposed to explain enhanced activity of DAAO towards L-amino acids under optimal pH condition.
研究领域:
生物化学
·
酶学
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高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2024
·
IF 5.4
Yonghai Liu,Pengfei Zhang,Peiyun Lei,Yige Jin,Haoting Yu,Xingde Zhang,Yonglan Pan,Chunyan Ou,Tingming Fu
The core to the treatment of gout is the elimination of pathologic crystal, monosodium urate monohydrate (MSUM). The primary treatment available is to gradually dissolve the “culprit crystals” by lowering the blood uric acid concentration with medications, which often takes a long time and in severe cases must still be treated surgically. Herein, we developed a dynamic bionic platform based on a hydrogel composite membrane (HCM) to screen the direct facilitated solubilization of MSUM crystals by small organic molecules in bionic saturated, or even supersaturated, solutions. The customized and biologically safe (NAGA/PEGDA/NIPAM) HCM, which is consistent with the main amino acid composition of articular cartilage, well mimics the entire process of organic molecules leading to the dissolution of MSUM crystals in the joint system. With the verifications of this platform, it is shown that l-aspartic acid (ASP) significantly promotes the dissolution of MSUM crystals not only in saturated but also in supersaturated solutions. Furthermore, a novel mechanism called “crane effect” was used to explain this “dissolution effect” of ASP on MSUM, which stems from the ability of ASP to lock onto the surface of MSUM crystals through hydrogen bonding by virtue of its two carboxyl groups , and simultaneously its amino group lifts the uric acid molecules from the surface of MSUM crystals by virtue of interactions of hydrogen bonding . The results of bulk crystallization , scanning electron microscopy (SEM), powder X-diffraction (PXRD), and density-functional theory (DFT) studies are quantitatively consistent with this hypothetical "crane effect" mechanism. Hence, this HCM-based functional platform could provide entirely novel ideas and methods for drug design and screening for the treatment of pathological crystal diseases of gout.
研究领域:
生物医学工程
·
药物设计
·
晶体病治疗
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高影响因子
International Journal of Materials Research
·
2023
·Vol. 114
·期 3
·207-218
·
IF 0.8
Xiaoyu Wang,Zizhan Sun,Chunhu Yu,Zhengyu Cai,Chuangang Fan,Lizhai Pei
Bi 2 O 3 /Dy 2 O 3 nanoflakes with triclinic Bi 2 O 3 and cubic Dy 2 O 3 phases were synthesized by a hexadecyl trimethyl ammonium bromide (CTAB)-assisted hydrothermal route. The Bi 2 O 3 /Dy 2 O 3 nanoflakes were analyzed by X-ray diffraction, X-ray photoelectron spectroscopy, electron microscopy and electrochemical impedance spectroscopy. The size of the Bi 2 O 3 /Dy 2 O 3 nanoflakes with curled surface is about 2 μm and thickness is about 25 nm. X-ray photoelectron spectroscopy confirms the chemical composition of the Bi 2 O 3 /Dy 2 O 3 nanoflakes. The formation process of the Bi 2 O 3 /Dy 2 O 3 nanoflakes was investigated by controlling the CTAB concentration, reaction temperature and reaction time. The formation of the Bi 2 O 3 /Dy 2 O 3 nanoflakes depends on CTAB. The results of cyclic voltammetry (CV) and electrochemical impedance spectroscopy demonstrate good electro-catalytic activity of the Bi 2 O 3 /Dy 2 O 3 nanoflakes towards L-cysteine with a pair of quasi-reversible CV peaks at +0.01 V and –0.68 V, respectively. Bi 2 O 3 /Dy 2 O 3 nanoflakes modified electrode detects L-cysteine linearly over a concentration ranging from 0.001 to 2 mM with a detection limit of 0.32 μM. The proposed nanocomposites modified electrode possesses good reproducibility and stability which can be used as a promising candidate for L-cysteine detection.
研究领域:
材料科学
·
电化学
·
生物传感器
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高影响因子
CHEMISTRY-A EUROPEAN JOURNAL
·
2021
·Vol. 27
·期 37
·9654-9660
·
IF 5.236
Lichao Wang,Lian Xu,Bingmei Su,Wei Lin,Xinqi Xu,Juan Lin
Graphical CASTing call : A semi-rational combinatorial active-site saturation test (CAST) was performed to improve the C β stereoselectivity of threonine aldolase. MD simulations suggested that the Y314R variant could interact with 4-methylsulfonyl benzaldehyde through hydrogen bonding to control the direction of entry of substrate, this increased the de value from 58 to 81 %. l -Threonine aldolase from Actinocorallia herbida ( Ah LTA) is an ideal catalyst for producing l - threo -4-methylsulfonylphenylserine [(2 S ,3 R )- 1 b ], a key chiral precursor for florfenicol and thiamphenicol. The moderate C β stereoselectivity is the main obstacle to the industrial application of Ah LTA. To address this issue, a combinatorial active-site saturation test (CAST) together with sequence conservatism analysis was applied to engineer the Ah LTA toward improved C β stereoselectivity. The optical mutant Y314R could asymmetrically synthesize l - threo -4-methylsulfonylphenylserine with 81 % diastereomeric excess ( de ), which is 23 % higher than wild-type Ah LTA. Molecular dynamic (MD) simulations revealed that the mechanism for the improvement in C β stereoselectivity of Y314R is due to the acylamino group of residues Arg314 controlling the orientation of substrate 4-methylsulfonyl benzaldehyde ( 1 a ) in the active pocket by directed interaction with the methylsulfonyl group; this leads to asymmetric synthesis of l - threo -4-methylsulfonylphenylserine. The success in this study demonstrates that direct control of substrates in an active pocket is an attract strategy to address the C β stereoselectivity problem of LTA and contribute to the industrial application of LTA.
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高影响因子
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