Microbiology Spectrum
·
2022
·Vol. 10
·期 4
·
IF 9.043
Mohamed Sharaf,Alaa H. Sewid,H. I. Hamouda,Mohamed G. Elharrif,Azza S. El-Demerdash,Afaf Alharthi,Nada Hashim,Anas Abdullah Hamad,Samy Selim,Dalal Hussien M. Alkhalifah,Wael N. Hozzein,Mohnad Abdalla,Taisir Saber
Surface-growing antibiotic-resistant pathogenic bacteria such as Escherichia coli and Staphylococcus aureus are emerging as a global health challenge due to dilemmas in clinical treatment. Furthermore, their pathogenesis, including increasingly serious antimicrobial resistance and biofilm formation, makes them challenging to treat by conventional therapy. Therefore, the development of novel antivirulence strategies will undoubtedly provide a path forward in combatting these resistant bacterial infections. In this regard, we developed novel biosurfactant-coated nanoparticles to combine the antiadhesive/antibiofilm properties of rhamnolipid (RHL)-coated Fe3O4 nanoparticles (NPs) with each of the p-coumaric acid (p-CoA) and gallic acid (GA) antimicrobial drugs by using the most available polymer common coatings (PVA) to expand the range of effective antibacterial drugs, as well as a mechanism for their synergistic effect via a simple method of preparation. Mechanistically, the average size of bare Fe3O4 NPs was ~15 nm, while RHL-coated Fe3O4@PVA@p-CoA/GA was about ~254 nm, with a drop in zeta potential from −18.7 mV to −34.3 mV, which helped increase stability. Our data show that RHL-Fe3O4@PVA@p-CoA/GA biosurfactant NPs can remarkably interfere with bacterial growth and significantly inhibited biofilm formation to more than 50% via downregulating IcaABCD and CsgBAC operons, which are responsible for slime layer formation and curli fimbriae production in S. aureus and E. coli, respectively. The novelty regarding the activity of RHL-Fe3O4@PVA@p-CoA/GA biosurfactant NPs reveals their potential effect as an alternative multitarget antivirulence candidate to minimize infection severity by inhibiting biofilm development. Therefore, they could be used in antibacterial coatings and wound dressings in the future.IMPORTANCE Antimicrobial resistance poses a great threat and challenge to humanity. Therefore, the search for alternative ways to target and eliminate microbes from plant, animal, and marine microorganisms is one of the world’s concerns today. Furthermore, the extraordinary capacity of S. aureus and E. coli to resist standard antibacterial drugs is the dilemma of all currently used remedies. Methicillin-resistant S. aureus (MRSA) and vancomycin-resistant S. aureus (VRSA) have become widespread, leading to no remedies being able to treat these threatening pathogens. The most widely recognized serotypes that cause severe foodborne illness are E. coli O157:H7, O26:H11, and O78:H10, and they display increasing antimicrobial resistance rates. Therefore, there is an urgent need for an effective therapy that has dual action to inhibit biofilm formation and decrease bacterial growth. In this study, the synthesized RHL-Fe3O4@PVA@p-CoA/GA biosurfactant NPs have interesting properties, making them excellent candidates for targeted drug delivery by inhibiting bacterial growth and downregulating biofilm-associated IcaABCD and CsgBAC gene loci.
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Frontiers in Microbiology
·
2024
·
IF 4
Song Zhenyan,He Jiawei,Yu Wenjing,He Chunxiang,Yang Miao,Li Ping,Li Ze,Jian Gonghui,Cheng Shaowu
Background: Schisanlactone E, also known as XueTongSu (XTS), is an active compound extracted from the traditional Tujia medicine Kadsura heteroclita (“XueTong”). Recent studies highlight its anti-inflammatory and antioxidant properties, yet the mechanisms of XTS’s therapeutic effects on Alzheimer’s disease (AD) are unclear. This study aims to elucidate the therapeutic efficacy and mechanisms of XTS in AD.Methods: Ten C57BL/6 mice were assigned to the control group (NC), and twenty APP/PS1 transgenic mice were randomly divided into the model group (M) (10 mice) and the XTS treatment group (Tre) (10 mice). After an acclimatization period of 7 days, intraperitoneal injections were administered over a 60-day treatment period. The NC and M groups received saline, while the Tre group received XTS at 2 mg/kg. Learning and memory abilities were assessed using the Morris Water Maze (MWM) test. Histopathological changes were evaluated using hematoxylin and eosin (HE) and Nissl staining, and immunofluorescence was used to assess pathological products and glial cell activation. Cytokine levels (IL-1β, IL-6, TNF-α) in the hippocampus were quantified by qPCR. 16S rDNA sequencing analyzed gut microbiota metabolic alterations, and metabolomic analysis was performed on cortical samples. The KEGG database was used to analyze the regulatory mechanisms of XTS in AD treatment.Results: XTS significantly improved learning and spatial memory in APP/PS1 mice and ameliorated histopathological changes, reducing Aβ plaque aggregation and glial cell activation. XTS decreased the expression of inflammatory cytokines IL-1β, IL-6, and TNF-α. It also enhanced gut microbiota diversity, notably increasing Akkermansia species, and modulated levels of metabolites such as isosakuranetin, 5-KETE, 4-methylcatechol, and sphinganine. Pathway analysis indicated that XTS regulated carbohydrate metabolism, neuroactive ligand-receptor interactions, and alanine, aspartate, and glutamate metabolism, mitigating gut microbiota dysbiosis and metabolic disturbances.Conclusion: XTS ameliorates cognitive deficits, pathological changes, and inflammatory responses in APP/PS1 mice. It significantly modulates the gut microbiota, particularly increasing Akkermansia abundance, and influences levels of key metabolites in both the gut and brain. These findings suggest that XTS exerts anti-AD effects through the microbial-gut-brain axis (MGBA).
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CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Yasir Abbas,Sajjad Ali,Majid Basharat,H.M. Adeel Sharif,Muhammad Ahsan Iqbal,Mohamed Bououdina,Haoliang Wang,Niaz Ali Khan
The sustainable recovery of gold from electronic waste (E-waste) remains a significant challenge due to lack of multiple redox active-sites in the existing adsorbents, their high cost, and poor stability in extreme acidic conditions. Herein, we rationally designed the skeleton engineering of poly(organo)phosphazene via substitution reaction of hexachlorocyclotriphosphazene (HCCP) with p-phenylenediamine (DA), 1,4-benzenedithiol (DT), and 4-aminothiophenol (AT), producing poly(p-phenylenediamine-co-cylotriphosphazene) (PDA) with N-redox active-sites, poly(1,4-benzenedithiol-co-cylotriphosphazene) (PDT) containing S-redox active-sites, and poly(4-aminothiophenol-co-cylotriphosphazene) (PAT) holding dual N-S-redox active-sites. The PDA, PDT, and PAT in the N-bromosuccinimide/pyridine (NBS/Py) leaching solution demonstrated extraction efficiency of 83.7 % Au 3+ , 45.5 % Cu 2+, 44 % Ni 2+ , and 83.6 % Au 3+ , 47.4 % Cu 2+ , 48 % Ni 2+ , and 83.1 % Au 3+ , 48.5 % Cu 2+ , 48.8 % Ni 2+ . While, in the aqua regia, these adsorbents showed extraction efficiency of 70.0 % Au 3+ , 14.0 % Cu 2+ , 13.8 % Ni 2+ , and 92.3 % Au 3+ , 6.4 % Cu 2+ , 9.8 % Ni 2+ , and 95.6 % Au 3+ , 13.0 % Cu 2+ , 10.3 % Ni 2+ . In particular, the PAT with the dual N-S-redox active sites achieved maximal Au 3+ extraction efficiency (99.2 %), which is 1.05 and 1.04 times higher than PDA and PDT within 15 min, superb uptakes of 2000 mg/g, excellent selectivity (99.9 %) in a mixture of eight metal ions (Al, Co, Cu, K, Mg, Na, Ni, Mn), 100 % removal capacity and high stability even in pure aqua regia leaching solution. Gold ions adsorbed on these adsorbents, spontaneously reduced, experienced nucleation, and grew into single crystalline nanoplates along (111) planes as well as nanolayers composed of Au-single-atoms and Au-nanoclusters. The density function theory (DFT) calculations and variation in binding energies of N and S indicated that due to the synergy of dual N-S-redox active-sites, the PAT showed enhanced performances and excellent selectivity. For commercial applications, the as-synthesized PDA, PDT, and PAT were highly stable in pure aqua regia leaching solution, showing precisely selective and rapid gold extraction (70 %, 92 %, 96 %, respectively) than N- NBS/Py leaching systems (83 %) with traces of other competing metals. This study proves that skeleton-modulated poly(organo)phosphazene greatly facilitates gold extraction for industrial-scale applications.
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Molecular Catalysis
·
2025
·
IF 4.9
Bo Fan,Run Lin,Xinyu Gu,Ruibing Cui,Ruiqing Yang,Yu-Cai He
Alcohols can be extensively used in the production of fine chemicals, such as fuel additives, food supplements and pharmaceutical intermediates, which can be acquired by the reduction of biomass-derived aldehyde. In this study, the recombinant Escherichia coli KPADH was genetically constructed through the co-expression of alcohol dehydrogenase from Kluyveromyces polysporus and glucose dehydrogenase from Bacillus subtilis . Biomass-derived aldehydes were biologically converted into alcohols in a benign system. In deep eutectic solvent (DES) choline chloride:glycerol-H 2 O (choline chloride:glycerol, 10 wt %) containing E. coli KPADH cells (0.1 g/mL), HMF (50 mM), glucose (2 equivalents), and NAD + (0.1 μM/mM HMF), bioreduction of HMF into 2,5-bis(hydroxymethyl)furan was implemented, acquiring a high yield of 98.1 % within 8 h. Other biobased compounds, such as isovanillin, vanillin, p -hydroxybenzaldehyde, furfural, and benzaldehyde were also converted into their corresponding alcohols. According to the molecular docking results, HMF had the lowest CDOCKER energy value of -27.422 kcal/mol. It was found that HMF displayed strong binding affinity with E. coli KPADH, resulting in enhanced binding stability and superior catalytic reduction activity. This study demonstrated a green and highly effective biocatalytic method for the synthesis of biobased alcohols.
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FOOD CHEMISTRY
·
2024
·
IF 8.5
Xiangxiang Sun,Yumei Yu,Zhenyu Wang,Kumayl Hassan Akhtar,Ahmed S.M. Saleh,Wenhao Li,Dequan Zhang
Effects of braising duration on volatile organic compounds (VOCs) and lipids in chicken were investigated. Aroma profiles identified by an electronic nose were effective in differentiating braising stages. During braising process, a total of 25 key VOCs were detected in braised chicken, and sample braised for 210 min exhibited the highest level of key VOCs. Additionally, a gas chromatography mass spectrometry fingerprint was established to evaluate the distribution of VOCs throughout the braising process. Partial least square discriminant analysis indicated that 2-heptanone, 3-methyl-2-butanone, octanal, nonanal, butanal, (E)-2-pentenal, 1-octen-3-ol, 1-hexanol, pentanal, hexanal, and 1-pentanol significantly affected flavor characteristics of braised chicken. Furthermore, 88 differential lipids were screened, and glycerolipids metabolic was found to be main metabolic pathway during braising process. Triglycerides (TG) and phosphatidyl ethanolamine (PE), such as TG (16:0/18:1/18:2), TG (18:0/18:1/18:2), TG (18:1/18:2/18:3), TG (18:1/18:1/18:2), PE (O-18:2/18:2), PE(O-18:2/18:1), and TG (16:0/16:1/18:2), played a vital role in the generation of VOCs.
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DOI
PubMed
高影响因子
Innovative Food Science & Emerging Technologies
·
2025
·
IF 6.8
Jingjing Li,Zehong Fang,Xueqing Huang,Yuhang Cai,Boxin Jiang,Giancarlo Cravotto,Zhengya Dong,Zhilin Wu
Ultrasonic emulsification frequently serves to prepare nanoemulsions containing active ingredients, including Vitamin E (VE), which can be degraded by Reactive Oxygen Species (ROS) generated during conventional ultrasonication. To address this issue, VE nanoemulsions were continuously prepared using a novel temperature-controlled ultrasonic tube-in-tube reactor (USTIT). Effects of formulation and process parameters on physical and chemical stability were systematically investigated. The mixing surfactant of Tween-80/Span-80 produced smaller, more stable, and uniform droplets (135 nm), remaining stable for 100 days. As the aqueous to oil (A/O) ratio increased, the droplet sizes decreased significantly, and the VE retention increased. With antioxidant Ascorbyl Palmitate (AP), the VE half-life reached 862 days at an A/O ratio of 15/1, significantly enhancing VE stability at room temperature. Moreover, VE degradation kinetics changed from first-order to second-order, leading to more persistent protection of VE by AP. In addition, lower ultrasonic power, higher flow rate, and higher temperature significantly reduced ROS yield and VE degradation. Based on the accelerating degradation kinetics of VE during a 60 °C holding period, VE nanoemulsions with AP, prepared at 45 W of ultrasonic power, 3 mL/min of flow rate, and at 50 °C, exhibited a long VE half-life of 205 h due to uniform ultrasonic energy distribution, and controllable residence time and temperature. In contrast, the VE half-life only reached 92.6 h by a conventional ultrasonic probe and 170.1 h by an ultrasonic bath. Therefore, the USTIT process provides a foundation for the continuous, stable preparation of high-value lipid-soluble bioactive nanoemulsions.
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FOOD RESEARCH INTERNATIONAL
·
2026
·
IF 8.8
Qi Zhao,Yicheng Zhou,Cong Wang,Mingxuan Li,Shan Zhu,Chang Xue,De Ji,Yuwen Qin,Chengxi Jiang
Gastrodia elata Bl. ( GE ), a widely utilized edible and functional material, exhibits notable quality variations due to distinct growing conditions across different geographical origins, making reliable methods for origin authentication and quality evaluation essential. This study integrated machine vision, flash GC e-nose and HPLC to analyze GE samples from Anhui (AH), Shaanxi (SN), and Yunnan (YN) provinces in China. Results showed comparable color characteristics among geographical origins, but distinct textural differences: SN samples displayed more complex textures with higher surface roughness, YN samples showed richer textural details but less distinct edges and lower periodicity, while AH samples exhibited smoother surfaces. Eleven flavor compounds were identified as potential flavor markers. Although no significant differences were found in the total content of six marker components, three linear machine learning models (PLS, SVM, and LDA) established based on multidimensional features achieved 100% classification accuracy in training and prediction sets, providing a reliable approach for GE geographical origin traceability.
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Food Chemistry-X
·
2026
·
IF 9.2
Jianyu Yuan,Miaomiao Tian,Wenting Chen,Macao Zhuo,Guang Li,Yue Wang
The low water solubility and poor thermal stability of Vitamin E (VE) limit its application in food processing. This study investigated the effects of the synergistic combination of electron beam irradiation (EBI) at doses of 0, 5, and 10 KGy and ultra-high pressure (UHP) at 100, 300, and 500 MPa on the preparation of potato starch-VE complexes. EBI pretreatment significantly reduced the degree of branching and enhanced complexation, antioxidant activity, and resistant starch (RS) content in a dose-dependent manner, whereas increasing UHP pressure progressively diminished these attributes. The combination of 10 KGy EBI with 100 MPa UHP yielded the highest RS content (67.24%) and DPPH scavenging activity (85.02%). XRD analysis indicated no V-type crystalline formation, suggesting weak but sustained complexation. The parameter-dependent effects of EBI and UHP offers a promising approach to produce starch-VE complexes with improved antioxidant capacity and digestion resistance, benefiting functional food development for diabetics.
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PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
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2026
·
IF 7.1
Qun-Yan Xiong,Hui-Wen Gu,Qing-Qing Luo,Chao Wang,Yan-Qing Fu,Xiao-Li Yin
A machine learning-driven strategy for optimal feature selection is proposed. • W1W and W1C sensors serve as key variables for aroma type classification. • 131 volatile compounds were identified in Liupao tea. • 14 aroma type differentiating compounds were identified. • 4 key variables yielded 100% accurate aroma recognition models. This study aimed to differentiate between the stale-aroma and betelnut-aroma types of Liupao tea by analyzing their volatile profiles. An integrated methodology was employed, combining electronic nose (E-nose) technology with headspace gas chromatography-ion mobility spectrometry (HS-GC-IMS). The E-nose data effectively differentiated between the two aroma types in both dry leaves and tea infusions. HS-GC-IMS enabled identification of 131 volatile compounds. Key discriminative features were selected through machine learning by integrating the variable importance in projection (VIP) from orthogonal partial least squares discriminant analysis (OPLS-DA) with Shapley additive explanations (SHAP) values from extreme gradient boosting (XGBoost). This process identified two critical E-nose sensor channels and two key volatile compounds. Utilizing these four optimal variables, three classification models were developed: principal component analysis-linear discriminant analysis (PCA-LDA), partial least squares discriminant analysis (PLS-DA), and support vector machine (SVM). All models demonstrated a perfect classification accuracy of 100% on both the training and internal test datasets. The PCA-LDA and PLS-DA models maintained 100% accuracy on the independent test set, whereas the SVM model achieved a classification accuracy of 80% on the same set. These results reveal the volatile characteristics of Liupao tea and offer valuable insights for quality assessment and targeted flavor modulation.
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Environmental Chemistry and Ecotoxicology
·
2026
·
IF 12.3
Yuxian Liu,Yutan Cai,Dongmei Li,Huajun Meng,Yufeng Lai,Jingtai Xu,Yongyin Lan,Xuan Zeng,Bo Zhang,Zhipeng Cheng,Tao Zhang,Jingchuan Xue
Modern e-waste dismantling workshops showed substantially elevated BTH and BTR levels in indoor dust. • 2-OH-BTH predominated in dust, reaching 8100 ng/g in modern e-waste dismantling workshops. • 2-OH-BTH and 1-H-BTR dominated urinary profiles, with 1.8-fold higher concentrations in e-waste workers than rural residents. • BTH EDIs via dust ingestion were ∼ 24-fold higher in workers, yet dust contributed ≤2.33% of total exposure. • Significant intra- and inter-class correlations were observed for BTHs and BTRs in both dust and urine. Benzothiazoles (BTHs) and benzotriazoles (BTRs) are high production volume chemicals widely incorporated into electronic products, yet their environmental occurrence and potential occupational exposure during e-waste recycling remain poorly characterized. In this study, four BTHs and three BTRs were quantified in 113 dust samples collected from a modern e-waste recycling facility and 15 household dust samples, as well as in urine samples from 121 e-waste dismantling workers and 20 rural residents. The median Σ(BTHs+BTRs) in modern e-waste (e.g., cell phone) dismantling workshop dust (3000 ng/g) was approximately 9–10 times higher than that observed in traditional e-waste (e.g., refrigerator) dismantling workshop (350 ng/g), e-waste storage areas (260 ng/g) and suburb residential homes (300 ng/g). These findings indicate substantial accumulation of BTHs and BTRs in indoor dust associated with modern e-waste dismantling activities. 2-Hydroxybenzothiazole (2-OH-BTH) was the predominant congener in e-waste associated dust, accounting for more than 84–95% of Σ(BTHs+BTRs) across different microenvironments, compared with 73% in reference residential dust, suggesting relatively modest differences in congener profiles between recycling and residential environments. In urine, 2-OH-BTH and 1H-BTR were the predominant detected congeners, accounting for 83% and 67% of Σ(BTHs+BTRs) in workers and rural residents, respectively. Their median urinary concentrations in workers were 4.3 and 1.8 ng/mL, respectively, representing 1.8-fold higher levels than those in rural residents (2.4 and 1.0 ng/mL). Significant positive correlations were observed among BTHs, among BTRs, and between the two chemical classes in both dust and urine, suggesting shared or related exposure sources. The median estimated daily intake of BTHs through dust ingestion among workers was 2.60 ng/kg bw/day, approximately 24-fold higher than that estimated for rural residents (0.11 ng/kg bw/day), indicating elevated occupational exposure potential. Nevertheless, dust ingestion accounted for no more than 2.33% of the total BTH exposure estimated from urinary biomonitoring, suggesting that other exposure pathways may make greater contributions to overall internal exposure.
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高影响因子
ACS Nano
·
2022
·
IF 18.027
Hualing He,Jinru Liu,Yushu Wang,Yuhang Zhao,Yi Qin,Zhenyu Zhu,Zhicai Yu,Jinfeng Wang
Firefighting protective clothing is an essential equipment that can protect firefighters from burn injuries during the firefighting process. However, it is still a challenge to detect the damage of firefighting protective clothing at an early stage when firefighters are exposed to excessively high temperature in fire cases. Herein, an ultralight self-powered fire alarm electronic textile (SFA e-textile) based on conductive aerogel fiber that comprises calcium alginate (CA), Fe3O4 nanoparticles (Fe3O4 NPs), and silver nanowires (Ag NWs) was developed, which achieved ultrasensitive temperature monitoring and energy harvesting in firefighting clothing. The resulting SFA e-textile was integrated into firefighting protective clothing to realize wide-range temperature sensing at 100–400 °C and repeatable fire warning capability, which could timely transmit an alarm signal to the wearer before the firefighting protective clothing malfunctioned in extreme fire environments. In addition, a self-powered fire self-rescue location system was further established based on the SFA e-textile that can help rescuers search and rescue trapped firefighters in fire cases. The power in the self-powered fire location system was offered by an SFA e-textile-based triboelectric nanogenerator (TENG). This work provided a useful design strategy for the preparation of ultralight wearable temperature-monitoring SFA e-textile used in firefighting protective clothing.
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PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2022
·
IF 9.136
Xi Zhang,Huaimeng Li,Mengxiang Ye,Haimin Zhang,Guozhong Wang,Yunxia Zhang
To solve metal resource shortage and alleviate environmental issues, it is in urgent need to explore cost-effective and environment-friendly adsorbents for sustainable recovery of valuable gold from e-waste. Herein, bacterial cellulose (BC) hybrid membrane grafted with adipic dihydrazide (ADH) has been fabricated via a facile schiff base reaction strategy for efficient and selective recovery of gold from complex aqueous media. The resulting [email protected] hybrid membrane features high ratio of hydrazide groups (67% of ADH by mass content) and hierarchical porous structure, ensuring high adsorption capacity (1149 mg/g) and fast adsorption equilibrium time (20 min) toward gold. Meanwhile, the remarkable recovery selectivity of gold is achieved in the simulated e-waste leachate containing a large amount of interfering metal ions. Notably, the captured Au(III) species on [email protected] hybrid membrane can be directly converted by redox-active hydrazide groups into Au(0) with no need of any external reductant. Furthermore, the developed [email protected] membrane possesses superior recycling and reusability performance, as reflected by no obvious decrease in gold extraction capability after six cycles. More importantly, the [email protected] hybrid membrane is capable of extracting almost 100% of gold from actual waste central processing unit (CPU) leachate, yielding high-purity gold (23.7 K) after pyrolysis.
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