SENSORS AND ACTUATORS B-CHEMICAL
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2026
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IF 8.3
Yannan Xue,Chen Li,Yuan Ma,Yongxin Hu,Meiyue Ma,Yixuan Wu,Lei Zhao,Wenxuan Jiang,Jiayu Yang,Xiao Liu,Tiantian Sun,Qi Gao,Shuai Meng,Qiang Liu,Tiehai Li,Chengli Zong
Conventional microarray chemistries are generally optimized for amine-bearing probes, whereas native polysaccharides and glycolipids often require prior derivatization for immobilization, which risks altering key carbohydrate epitopes. Here, we developed a dual-anchor microarray platform ( DAMP ) by modifying commercially available poly-L-lysine-coated slides with NHS esters and diazirine photo-crosslinkers. The key innovation of this platform is the complementary dual-chemistry design on a high-loading 3D surface: NHS chemistry enables efficient immobilization of amine-bearing probes, whereas diazirine-mediated photo-crosslinking enables derivatization-free capture of underivatized native glycans. The resulting platform enabled rapid immobilization of native polysaccharides and glycolipids under mild UV irradiation and showed markedly enhanced sensitivity for amine-bearing glycans:10 pg/mL for a synthetic glycan and 0.32 ng/mL for purified pneumococcal cell wall polysaccharide. DAMP also supported the immobilization of 29 structurally distinct carbohydrates with retained lectin recognition, as well as direct analysis of 13 native capsular polysaccharides for pneumococcal serotyping and vaccine-response profiling. This work establishes a practical and sensitive surface platform for high-fidelity carbohydrate immobilization and expands the utility of microarrays in polysaccharide research and glycan-based vaccine studies.
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MICROCHEMICAL JOURNAL
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2025
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IF 5.1
Sibo Wang,Qiu Sun,Xuelin Zhang,Huiyang Xu,Weijian Yuan,Jianfeng Wu
Continuous non-invasive glucose monitoring remains challenging due to the lack of robust sensing interfaces that simultaneously ensure mediator stability, high sensitivity, and resistance to complex biofluid matrices. To address this, we develop a wearable electrochemical biosensor leveraging laser-induced graphene (LIG) functionalized via a π–π stacking-assisted covalent conjugation strategy. Unlike conventional approaches requiring corrosive electrode activation, our method exploits 1-pyrenebutyric acid N-hydroxysuccinimide ester (PBASE) as a molecular bridge to anchor aminoferrocene (AFc) mediators on pristine LIG through non-destructive π–π stacking followed by stable amide bonding. Glucose oxidase (GOx) is encapsulated in a chitosan matrix, forming a biocompatible sensing interface.The fabricated sensor exhibited a high electrochemically active surface area and fast electron transfer kinetics. Under optimized conditions, the sensor demonstrated a optimally wide linear range (0–1.0 mM), high sensitivity (32.1 μA mM −1 cm −2 ), and a low detection limit (13 μM). The device showed excellent selectivity against common sweat interferents, good reproducibility (RSD = 2.85 %), and long-term stability. Standard addition recovery tests in artificial sweat yielded recoveries between 93.7 % and 105.3 %, confirming its practical applicability. This work resolves the long-standing trade-off between stable mediator immobilization and electrode integrity, providing a scalable platform for reliable diabetes management.
研究领域:
电化学生物传感器
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可穿戴电子设备
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糖尿病管理
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纳米材料
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生物分析化学
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Scientific Reports
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2017
·Vol. 7
·期 1
·1-11
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IF 4.259
Zhou Yang,Yuan Shaofei,Liu Qian,Yan Dandan,Wang Yun,Gao Li,Han Juan,Shi Haifeng
In this paper, an efficient and convenient Fe 3 O 4 /PMG/IDA-Ni 2+ nanoparticles that applied to purify and immobilize his-tagged β-glucosidase was synthesized, in which, Fe 3 O 4 /PMG (poly ( N, N’- methylenebisacrylamide-co-glycidyl methacrylate) core/shell microspheres were synthesized firstly using distillation-precipitation polymerization, then iminodiacetic acid (IDA) was used to open epoxy rings on the shell of microspheres to the combination of Ni 2+ . The gene of β-glucosidase that was from Coptotermes formosanus Shiraki was amplified, cloned into the expression vector pET28a with an N-terminal His-tag, and expressed in E.coli BL21. The nanoparticles showed the same purification efficiency as commercial nickel column which was a frequently used method in the field of purifying his-tagged proteins from crude cell lysates. The results indicated that Fe 3 O 4 /PMG/IDA-Ni 2+ nanoparticles can be considered as an excellent purification material. β-glucosidase was immobilized on the surface of Fe 3 O 4 /PMG/IDA-Ni 2+ to form Fe 3 O 4 /PMG/IDA-β-glucosidase by means of covalent bound with imidazolyl and Ni 2+ . The immobilized β-glucosidase exhibited excellent catalytic activity and stabilities compared with free β-glucosidase. In addition, immobilized β-glucosidase can be recycled for many times and retain more than 65% of the original activity. The materials display enormous potential in the aspect of purifying and immobilizing enzyme.
研究领域:
生物化学
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生物技术
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酶学
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纳米技术
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高影响因子
ANALYTICAL SCIENCES
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2019
·Vol. 35
·期 9
·967-972
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IF 1.618
Wang Wen-Wen,Han Xiao,Chu Li-Qiang
Nucleic acid aptamers have been widely used as synthetic probes for bioanalytical applications. Herein, we carried out a detailed study on the immobilization of a series of aptamers ranging from 37 to 88 bases, which are specific to either Escherichia coli ( E. coli ) or Staphylococcus aureus ( S. aureus ), on a planar gold substrate via a polyadenine-mediated immobilization method. The resultant surfaces were characterized by both surface plasmon resonance spectroscopy (SPR) and X-ray photoelectron spectroscopy. The results clearly show that the aptamer solution at a lower ionic strength gives rise to a higher lateral density of the aptamer when compared to that at a higher ionic strength. The SPR aptasensors are then employed for detecting their corresponding bacteria ( i.e., E. coli and S. aureus , respectively). The data indicate that the SPR aptasensor with a higher density of aptamer exhibits a better capture of target bacteria.
研究领域:
生物化学
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分析化学
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生物传感器
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微生物学
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JOURNAL OF HAZARDOUS MATERIALS
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2021
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IF 10.588
Jianle Wang,Xueming Liu,Zhihua Zhu,Le Yuan,Dongye Zhao,Hong Deng,Zhang Lin
High concentrations of Cr(VI) are often detected in contaminated soil. Yet, cost-effective remediation technologies have been lacking. In this study, we prepared a type of FeS x based on commercial FeSO 4 .7H 2 O and CaS x and tested a microwave-assisted technology based on FeS x for reductive immobilization of high concentrations of Cr(VI) in a field contaminated soil. The as-prepared FeS x particles appeared as a honeycomb-like and highly porous structure. The microwave-assisted FeS x reduction process was able to rapidly reduce the TCLP-based reachability of Cr(VI) from 391.8 to 2.6 mg·L -1 . The dosage of FeS x , S/Fe molar ratio, initial moisture content, microwave power, and irradiation time can all affect the treatment effectiveness. After 500 days curing under atmospheric conditions, the TCLP-leached concentration of Cr remained below the regulatory limit of 5 mg·L -1 , while other treatments failed to meet the goal. S x 2- or S 2- served as the primary electron donors, whereas Fe facilitated the microwave absorption and the formation of the stable final product of FeCr 2 O 4 . S and Fe are mostly precipitated in soil. The microwave-assisted FeS x reduction was shown to be an effective approach to rapidly reduce the leachability of Cr(VI) in contaminated soil, especially in heavily contaminated soil.
研究领域:
环境科学
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土壤修复
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化学工程
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ENZYME AND MICROBIAL TECHNOLOGY
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2022
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IF 3.705
Yun-Tao Zhao,Kun Zhang,Jian Zeng,Haowen Yin,Wenjing Zheng,Rui Li,Anzi Ding,Shaohong Chen,You Liu,Wenjin Wu,Zhanxin Jing
Enzyme immobilization technology has a key role in improving the stability of enzyme reaction systems and biocatalyst utilization rates. In this study, polyvinyl alcohol/sodium alginate@Fe 3 O 4 (PVA/SA@Fe 3 O 4 ) magnetic immobilized-enzyme hydrogel beads were prepared. Their structure and morphology were characterized by scanning electron microscopy, surface area and porosity analyses, Fourier transform infrared spectroscopy , X-ray photoelectron spectroscopy and a vibrating sample magnetometer. The capability of PVA/SA@Fe 3 O 4 to adsorb neutral protease was investigated with variations in composition, temperature, pH, stirring speed, enzyme concentration, and crosslinking concentration. The optimal parameters of the immobilization process were determined by response surface methodology (3% neutral protease, 4% crosslinking at 200 rpm, 45 °C and pH 7.2), under which an immobilization rate of 41.98 mg/g was obtained. The thermal stability , acid-base stability, and reusability of the immobilized enzyme were improved significantly. After seven cycles, the immobilized enzyme activity remained at 30.8% that of the initial enzyme activity . The results indicate that the immobilization of NP onto magnetic PVA/SA@Fe 3 O 4 hydrogel beads improves enzyme efficiency, giving this process potential industrial applications.
研究领域:
生物化学
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酶工程
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高影响因子
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
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2021
·Vol. 26
·期 6
·910-922
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IF 2.836
Chen Yi,Zhao Min,Hu Liyong,Wang Zeyu,Hrynsphan Dzmitry,Chen Jun
Acrylic acid has been widely used in various industrial applications but is harmful to human health and the environment. A novel and efficient degrading acrylic acid bacterium was isolated and identified as Bacillus aryabhattai CY. In this study, batch experiments were conducted to evaluate the biodegradation of acrylic acid by B. aryabhattai CY, which were immobilized in calcium-alginate beads under different conditions. The components of the alginate beads were optimized by the response surface method, and the degradation performance of the immobilized cells was determined. Relative to the free cells, experiment results showed that the immobilized cells can achieve complete degradation of 100 mg/L acrylic acid in 24 h under the optimal conditions of SA 6% (w/v), CaCl 2 1% (w/v), and immobilization time of 6 h. According to Haldane’s model, the maximum specific growth rate ( μ max ) of the free cells and immobilized cells were 0.165/h and 0.210/h, respectively. Experiment data revealed that acrylic acid showed an inhibitory effect on biodegradation by B. aryabhattai CY, especially at concentration higher than 100 mg/L. Furthermore, the reusability of the immobilized cells revealed that the acrylic acid removal rate was above 93.70% within the eight cycles. The immobilized cells also showed higher removal efficiencies in wider ranges of temperature (20°C–60°C) and pH (5.0–10.0) than the free cells. Moreover, the possible degradation intermediates were proposed during the biodegradation of acrylic acid by GC-MS analysis. Results indicated that immobilized beads might have a potential environmental implication in the purification of practical acrylic acid wastewater.
研究领域:
环境生物学
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微生物学
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SEPARATION AND PURIFICATION TECHNOLOGY
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2023
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IF 8.6
Keren Lu,Zhiren Zhao,Jin Cui,Cuiyun Bai,Hongling Zhang,Xuetao Zhao,Fengyun Wang,Mingzhu Xia,Yanru Zhang
The magnetic Hexamethylene diamine tetramethylene phosphonic acid (HDTMP)-hydroxyapatite nanoparticles (HD-MHAP) were firstly prepared and applied for the immobilization of radioactive Sr 2+ (Strontium ion) and Co 2+ (Cobalt ion). The molar ratio of organic phosphine in HDTMP to total phosphorus was 10% (10HD-MHAP) and can also be easily separated from aqueous solution. At the equilibrium concentration of Sr 2+ and Co 2+ (200 mg/L), the adsorption capacity of MHAP and 10HD-MHAP on Co 2+ was 28.4 mg/g and 172.3 mg/g, respectively and for Sr 2+ , the adsorption capacity was 54.9 mg/g and 320.7 mg/g. Additionally, the desorption percentage of Sr 2+ (%) and Co 2+ (%) was very low, which was 0.66 and 1.61, respectively. This is significant because the irreversible adsorption of HDTMP/Fe 3 O 4 /HAP composite for Sr 2+ and Co 2+ ensures the captured radionuclides will not be released back into the aquatic systems. Moreover, the affinity between HDTMP and HAP and the coordination of HDTMP and Sr 2+ or Co 2+ was probed by Multiwfn wavefunction analysis. The results indicated that the formation of a ternary surface complex of MHAP-HDTMP-M (Sr 2+ or Co 2+ ) was the key to the excellent immobilization performance of HD-MHAP composite for Sr 2+ or Co 2+ .
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CARBOHYDRATE POLYMERS
·
2023
·
IF 11.2
Guodong Liu,Faxing Zou,Wei He,Junfei Li,Yajie Xie,Mengjiao Ma,Yudong Zheng
Bacterial cellulose (BC) has good network structure, biocompatibility, and excellent mechanical properties, and is widely used in the field of biomaterials. The controllable degradation of BC can further broaden its application. Oxidative modification and cellulases may endow BC with degradability, but these methods inevitably lead to the obvious reduction of its initial mechanical properties and uncontrolled degradation. In this paper, the controllable degradation of BC was realized for the first time by using a new controlled release structure that combines the immobilization and release of cellulase. The immobilized enzyme has higher stability and is gradually released in the simulated physiological environment, and its load can control the hydrolysis rate of BC well. Furthermore, the BC-based membrane prepared by this method retains the favorable physicochemical performance of the original BC, including flexibility and great biocompatibility, and holds good application prospects in drug control release or tissue repair.
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高影响因子
MOLECULES
·
2023
·Vol. 28
·期 6
·2569
·
IF 4.6
Yu Liang,Xinyan Chen,Jianli Zeng,Junqing Ye,Bin He,Wenjin Li,Jian Sun
Laccase immobilization is a promising method that can be used for the recyclable treatment of refractory phenolic pollutants (e.g., chlorophenols) under mild conditions, but the method is still hindered by the trade-off limits of supports in terms of their high specific surface area and rich functional groups. Herein, confined polymerization was applied to create abundant amino-functionalized polymeric ionic liquids (PILs) featuring a highly specific surface area and mesoporous structure for chemically immobilizing laccase. Benefiting from this strategy, the specific surface area of the as-synthesized PILs was significantly increased by 60-fold, from 5 to 302 m2/g. Further, a maximum activity recovery of 82% towards laccase was recorded. The tolerance and circulation of the immobilized laccase under harsh operating conditions were significantly improved, and the immobilized laccase retained more than 84% of its initial activity after 15 days. After 10 cycles, the immobilized laccase was still able to maintain 80% of its activity. Compared with the free laccase, the immobilized laccase exhibited enhanced stability in the biodegradation of 2,4-dichlorophenol (2,4-DCP), recording around 80% (seven cycles) efficiency. It is proposed that the synergistic effect between PILs and laccase plays an important role in the enhancement of stability and activity in phenolic pollutant degradation. This work provides a strategy for the development of synthetic methods for PILs and the improvement of immobilized laccase stability.Keywords:polymeric ionic liquids;confined polymerization;laccase;immobilization;2,4-DCP;removal
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APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2023
·
IF 3
Zhao Xia,Guo Meijing,Li Xian,Liu Bo,Li Binglin,Wang Jiao
Phospholipase D (PLD) with the higher transphosphatidylation activity was screened from Streptomyces sp. LD0501 basing on the protoplast mutagenesis technology. Then, it was successfully bio-imprinted to form a hyperactivated structure and rigidified by the intramolecular cross-linking, which was immobilized on the nonporous nanoscale silica. Characterization techniques were employed to investigate the structure and physicochemical properties of the catalysts, including Fourier transform infrared (FTIR) spectra and scanning electron microscopy (SEM) analysis. Transphosphatidylation activity and selectivity were improved significantly when immobilized PLD was used. The maximum yield for the production of phosphatidylserine (PS) reached 97% and the side reaction, the hydrolysis, was minimized. These results were further confirmed by the nuclear magnetic resonance (NMR) and mass spectrometry (MS) analysis. The imprint-induced characteristics of PLD was successfully “remembered” even in the present of much water. In addition, this immobilized hyperactivated PLD showed the excellent operational stabilities and environmental tolerances. Graphical abstract
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Molecular Catalysis
·
2023
·
IF 4.6
Xiuqin Dong,Chen Xin,Lingtao Wang,Hao Gong,Yifei Chen
Immobilization of the Rh active sites in novel porous materials has attracted attentions for solving the problems of separation and recycling of homogeneous catalysts. In this work, a comparative study of phosphine modified 1Rh/MOF-5 using three different methods was reported, namely 1Rh-P/MOF-5, 1Rh/MOF-5-P and 1Rh/MOF-5-PPh 3 . Similar with the traditional methods, PPh 3 was etched on Rh particles by impregnation in 1Rh-P/MOF-5 and encapsulated in the pores by solvothermal method in 1Rh/MOF-5-P. Moreover, we proposed a novel post-synthesis strategy for grafting phenyl phosphine on the organic linkers in 1Rh/MOF-5-PPh 3. The position of phenyl phosphine in MOFs and the effect of phosphine on the Rh active sites in hydroformylation were investigated by XRD, IR, XPS, TGA and DRIFTS. 1Rh/MOF-5-PPh 3 exhibited the best catalytic activity and stability than 1Rh-P/MOF-5 and 1Rh/MOF-5-P in the hydroformylation of 1-butene. Different P/Rh ratio in P-modified 1Rh/MOF-5 led to the spatial environment change around the Rh active sites. This study showed details in the role of phosphine ligands played in hydroformylation reactions and provided some guidance for MOFs materials in the immobilization of homogeneous catalysts.
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危险品化学品经营许可证（带存储）