MEASUREMENT
·
2025
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IF 5.6
Jintao Liang,Zhenshun Wei,Qing Huang,Jianlu Liang,XiuChuan Zhao,Zhide Zhou,Guiyin Li
Tea polyphenols are a general term for the total phenolic compounds in tea. Tea polyphenols play a crucial role in the quality of tea. Rapid detection of tea polyphenols is essential for tea harvesting and breeding. In this paper, a rapid method for the detection of tea polyphenols is presented based on diazotization coupling (DC) reaction and flow batch analysis system (FBAS). The tea polyphenols detection based on DC reaction can avoid interference from non-phenolic substances and improve detection accuracy. Meanwhile, FBAS for sample injection can shorten the analysis time, reduce the reagent consumption and improve the sensitivity. Experimental results demonstrate that absorbance values exhibit linearity with gallic acid concentrations in the range of 5 to 60 μg/mL, with a linear equation of y = 0.0279x + 0.0613 (R 2 = 0.9993) and a limit of detection (LOD) of 0.215 μg/mL (S/N=3). The method was applied to the detection of tea polyphenols in tea samples, with relative standard deviations (RSD) ranging from 0.326% to 0.659%. The DC-FBAS method has a high potential application with the requirements of portable, cost-effective, and highly precise on-site tea polyphenols detection.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Fang Kou,Weihao Wang,Sangguan You,Xuetuan Wei,Xian Wu
A novel encapsulation system was designed, utilizing sodium alginate (SA) polysaccharide as the matrix and easily absorbed Fe 2+ as the metal-organic framework, to construct microbead scaffolds with both high catechins (CA) and vitamin C (Vc) loading and antioxidant properties. The structure of microbead hydrocolloids was investigated using SEM, XPS, FTIR, XRD and thermogravimetry, and the antioxidant activity , in vitro digestion and the release of CA and Vc were evaluated. These results revealed that the microbead hydrocolloids SA-CA-Fe and SA-CA-Vc-Fe exhibited denser and stronger cross-linking structures, and the formation of inter- and intramolecular hydrogen and coordination bonds improved thermal stability . Moreover, SA-CA-Fe (44.9 % DPPH and 47.8 % ABTS) and SA-CA-Vc-Fe (89.9 % DPPH and 89.3 % ABTS) displayed strong antioxidant activity . Importantly, they were non-toxic in Caco2 cells. The SA-CA-Fe and SA-CA-Vc-Fe achieved significantly higher CA (56.9 and 62.7 %, respectively) and Vc (42.2 %) encapsulation efficiency while maintaining higher CA and Vc release in small intestinal environment. These results suggested that SA polysaccharide-based encapsulation system using Fe 2+ framework as scaffold had greater potential for delivery and controlled release of CA and Vc than conventional hydrocolloids, which could provide new insights into the construction of high loading, safe, targeted polyphenol delivery system.
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Foods
·
2024
·Vol. 13
·期 22
·3609
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IF 4.7
Ning Hua,Xian’e Ren,Feng Yang,Yongchun Huang,Fengyan Wei,Lihui Yang
In this study, hydrodynamic cavitation technology was utilized to prepare conjugates of soy protein isolate (SPI) with polyphenols, including resveratrol (RA) and polydatin (PD) from the stilbene category, as well as arctiin (AC) and magnolol (MN) from the lignan category. To investigate the effects of hydrodynamic cavitation treatment on the interactions between SPI and these polyphenols, the polyphenol binding capacity with SPI was measured and the changes in the exposed sulfhydryl and free amino contents were analyzed. Various methods, including ultraviolet–visible spectroscopy, fluorescence spectroscopy, Fourier transform infrared spectroscopy, and circular dichroism spectroscopy, were also used to characterize the structural properties of the SPI–polyphenol conjugates. The results showed that compared to untreated SPI, SPI treated with hydrodynamic cavitation exposed more active groups, facilitating a greater binding capacity with the polyphenols. After the hydrodynamic cavitation treatment, the ultraviolet–visible absorption of the SPI–polyphenol conjugates increased while the fluorescence intensity decreased. Additionally, the content of exposed sulfhydryl and free amino groups declined, and changes in the secondary structure were observed, characterized by an increase in the α-helix and random coil content accompanied by a decrease in the β-sheet and β-turn content. Furthermore, the SPI–polyphenol conjugates treated with hydrodynamic cavitation demonstrated improved emulsifying characteristics and antioxidant activity. As a result, hydrodynamic cavitation could be identified as an innovative technique for the preparation of protein–phenolic conjugates.
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FOOD HYDROCOLLOIDS
·
2025
·
IF 12.4
Songyu Wang,Kefan Ouyang,Ziqi Chen,Zhiyuan Huang,Yuanyuan Feng,Zihang Yan,Qin Zhang,Qiang Zhao
This study aimed to suppress lipid-protein co-oxidation in plant-based emulsions through covalent or non-covalent conjugation of resveratrol (RES) or luteolin (LUT) with potato protein isolate (PPI), and to develop functional whipped cream alternatives. Covalent conjugates (C-LUT/C-RES) showed higher polyphenol grafting efficiency non-covalent systems. The presence of a catechol group in LUT likely facilitated polyphenol grafting rate and enhanced radical scavenging, resulting in C-LUT emulsions exhibiting 26% lower TBARS (16.07 mg MDA/kg) and 44.18% less protein carbonyl formation after 15 days, superior to C-RES and unmodified PPI. C-RES demonstrated greater protection against protein oxidation, highlighting structure-function differences. C-LUT emulsions displayed improved rheology (higher viscosity and G′) and, when formulated into whipped cream, reduced whipping time to 27.33 s (vs. 240.33 s for Milk), increased hardness by 33.3%, chewiness by 12.6-fold, and springiness by 17.64%, while reducing leakage by 3.01%. In vitro digestion revealed 16.48% lower free fatty acid release and smaller particle size (1.28 μm, 65.6% of PPI), indicating delayed lipid hydrolysis. These findings demonstrate that C-LUT synergistically enhances oxidative stability, textural performance, and nutritional modulation, offering a promising strategy for engineering plant proteins in next-generation dairy alternatives.
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ACS Applied Polymer Materials
·
2025
·
IF 4.7
Yujiao Li,Xuemeng Hu,Zihan Wu,Shaoyuan Shi,Yingxue Sun
Based on similar chemical structures and reactive sites with dopamine, polyphenols and some amines can react and generate adhesive polymers through oxidation and Michael addition/Schiff base reactions. In this study, four kinds of polyphenol-amine codeposition solutions were applied in surface modification of anion exchange membranes used in electrodialysis (ED). Catechol (CA)/tannic acid (TA) and m-phenylenediamine (MPD)/polyethylenimine (PEI) were respectively selected as polyphenol monomer and amine monomer, with chemical structural differences in the length of the carbon chain, the number of benzene rings, and functional groups. The effects of monomer type on copolymerization and codeposition process were investigated. Results showed that polyphenol types had important influences on chemical reactions and deposition. The codeposition solutions containing CA, especially the CA-MPD solution with two small molecular monomers, exhibited active and fast reaction rates, reflected in the changes in solution color and UV–visible absorption. By contrast, the flocculent between macromolecular monomers of TA and PEI changed the presence of solution, intermediates, and polymeric products, promoting deposition rates. In ED experiments, all modified AEMs maintained the desalination efficiency and demonstrated a better antifouling ability. However, the modified AEMs could not totally resist foulants and protect from the negative effects of fouling; regulating and improving codeposition modification need more investigation.
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ADVANCED MATERIALS
·
2024
·
IF 27.4
Ying Kong,Xu Chen,Feng Liu,Jiageng Tang,Yijing Zhang,Xiangxiang Zhang,Luyao Zhang,Tong Zhang,Yaqi Wang,Mengxiao Su,Qixin Zhang,Hanxiang Chen,Di Zhou,Fan Yi,Hong Liu,Yi Fu
As a central metabolic molecule, nicotinamide adenine dinucleotide (NAD + ) can potentially treat acute kidney injury (AKI) and chronic kidney disease (CKD); however, its bioavailability is poor due to short half-life, instability, the deficiency of targeting, and difficulties in transmembrane transport. Here we designed a physiologically adaptive gallic acid-NAD + nanoparticle, that had ultrasmall size and pH-responsiveness, passed through the glomerular filtration membrane to reach injured renal tubules, and efficiently delivered NAD + into the kidneys. With an effective accumulation in the kidneys, it restored renal function, immune microenvironment homeostasis, and mitochondrial homeostasis of AKI mice via the NAD + -Sirt1 axis, and exerted strong antifibrotic effects on the AKI-to-CKD transition by inhibiting TGF-β signaling. It also exhibited excellent stability, biodegradable, and biocompatible properties, ensuring its long-term safety, practicality, and clinical translational feasibility. The present study shows a potential modality of mitochondrial repair and immunomodulation through nanoagents for the efficient and safe treatment of AKI and CKD. This article is protected by copyright. All rights reserved
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SCIENTIA HORTICULTURAE
·
2025
·
IF 4.2
Jianbin Shi,Dongfang Fang,Yong Sui,Tian Xiong,Xueling Chen,Chuanhui Fan,Deshun Zhou,Fang Cai,Xin Mei
Sweet potato ( Ipomoea batatas L.) leaves are rich in polyphenols and consumed as leaf vegetable in world. The content and composition of polyphenols are diversity at different harvesting period. This study investigated the changes of total polyphenols content (TPC), antioxidant capacity, and polyphenol fractions content of sixteen varieties of SPLs during growth stage. The results showed significant diversity in the concentrations of active compounds in different genotypes and growth stages. TPC ranged from 27.45 to 243.82 mg CAE/g DW among the varieties over various growth stages, with G3 and E1 varieties exhibiting relatively high TPC and antioxidant capacity. Twelve polyphenol fractions were identified in SPLs. Correlation analysis demonstrated a significant positive relationship between antioxidant capacity and the content of monosubstituted caffeoylquinic acids (3-caffeoylquinic acid, 5-caffeoylquinic acid, 4-caffeoylquinic acid) and disubstituted caffeoylquinic acids (3,4-di-O-caffeoylquinic acid, 3,5-di-O-caffeoylquinic acid, 4,5-di-O-caffeoylquinic acid). Principal component analysis highlighted the superior performance of the E1 variety in terms of polyphenol content and antioxidant capacity.
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CARBOHYDRATE POLYMERS
·
2024
·
IF 10.7
Xin Jin,Chengxiong Wei,Kai Li,Peinan Yin,Chengwei Wu,Wei Zhang
Wound healing is a complex process involving a complicated interplay between numerous cell types and vascular systems. Hyaluronic acid (HA)-based hydrogel facilitates wound healing, and is involved in all processes. However, slow gelation speed and weak adhesion strength limit its ability to form a stable physical barrier quickly. Herein, we propose a HA-based composite hydrogel as the wound dressing based on oxidative coupling reaction. Tannic acid and dopamine-coated carbon particles (DCPs) containing abundant phenolic hydroxyl groups are incorporated into the HA-based hydrogel for increasing the number of crosslinking sites of oxidative coupling of the hydrogel and enhancing adhesion through the formation of covalent bonds and hydrogen bonds between hydrogel and wound sites. The composite hydrogel exhibits short gelation time (<6 s) and high adhesion strength (>8.1 kPa), which are superior to the references and commercial products of its kind. The in vitro experiments demonstrate that the hydrogel has low hemolytic reaction, negligible cytotoxicity, and the ability to promote fibroblast proliferation and migration. The in vivo full-thickness skin defect model experiments demonstrate that the hydrogel can accelerate wound healing under mild photothermal stimulation of DCPs by reducing inflammation, relieving tissue hypoxia, and promoting angiogenesis and epithelialization.
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JOURNAL OF MEMBRANE SCIENCE
·
2024
·
IF 8.4
Weiguo Kong,Longfei Zhang,Na Yang,Bin Jiang,Luhong Zhang,Yong Liu
Rational design of hydrophilic hierarchical structures on the membrane surface is a promising method to improve oil/water separation efficiency. However, oil droplets with intrinsic adhesion tend to accumulate on the membrane surface, which deteriorates membrane performance. In this study, tannic acid-Zn(II) complex was homogeneously anchored on the PVDF membrane surface and further in situ converted to ZIF-8 in the presence of 2-methylimidazole. Subsequently, Cu ion exchange was employed to transfer ZIF-8 layer to gerhardtite (Cu 2 (OH) 3 NO 3 ) with a hierarchical structure. Accordingly, the as-prepared PVDF/TA-gerhardtite membrane possessed attractive hydrophilicity and underwater superoleophobicity with oil contact angles above 150°, therefore achieving over 98 % oil rejection towards various surfactant stabilized oil-in-water emulsions. Moreover, self-cleaning properties can be realized by activating peroxymonosulfate, and 97 % of tetracycline hydrochloride, the target pollutant, was degraded within 30 min owing to the generation of sulfate radicals and hydroxyl radicals. Due to the attractive hydrophilicity and catalytic oxidation capacity of the gerhardtite hierarchical structure, degradation and bubble generation induced fouling recovery was accomplished after PMS cleaning, and the irreversible fouling decreased to 2.2 % after consecutive soybean oil fouling. Therefore, this work provides new insights into the construction of hierarchical structures on membrane surfaces and demonstrates their potential applications in oil/water separation and fouling mitigation.
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FOOD HYDROCOLLOIDS
·
2024
·
IF 11
Ao Huang,Shunjing Luo,Fangjian Ning,Jiangping Ye,Chengmei Liu
This study investigated the effect of protein-polyphenol-polysaccharide ternary complexes prepared from polysaccharides with different charges on emulsion stability. Sodium alginate (ALG), chitosan hydrochloride (CHC) and oat β-glucan (OG) were used as representational polysaccharides. The interfacial rheology showed that CHC increased the adsorption rate of WPI-rutin complexes at the oil-water interface, while ALG and OG reduced the adsorption rate. Furthermore, WPI-Rutin-CHC complexes effectively reduced the interfacial tension from 11.16 mN/m to 9.41 mN/m. Compared with other ternary complexes, the oil-water interface layer composed of WPI-Rutin-CHC complexes had the highest interfacial viscoelasticity and mechanical strength. Moreover, the emulsion prepared by WPI-Rutin-CHC complexes had the lowest instability index (0.17) and the smallest droplet size (660 nm), indicating the highest stability among all the emulsions. The microstructure results indicated that the thick interfacial layer was observed in WPI-Rutin-CHC emulsion, while thin interfacial layers were found in other emulsions. These results revealed the stability mechanism of emulsions prepared by ternary complexes and provided guidance for the preparation of stable emulsions.
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Journal of Water Process Engineering
·
2025
·
IF 6.7
Hanjun Wu,Shiyin Gui,Zhenyue Zhang,Wenda Guo,Xiangyi Deng,Junxia Yu
In this work, chitosan was modified by extracting the polyphenols rich in pomegranate peels to prepare the bio-composite material (PPPs-CS), which was applied to capture the dispersed metal germanium (Ge(IV)) in wastewater. The experimental results indicate that PPPs-CS exhibits the best adsorption performance for Ge(IV) at pH 7.0 with a dosage of 0.7 g/L. The adsorption behavior conforms to the pseudo-second-order kinetics and the Langmuir model, with the maximum adsorption capacity fitted by the Langmuir model reaching 71.34 mg/g. The results of dynamic adsorption mass transfer simulations showed that the capture behavior of PPPs-CS on Ge(IV) was dominated by the chemical adsorption of monomolecular layer. The adsorption partition coefficient of PPPs-CS for Ge(IV) with coexisting metal ions of Mn(II), Cd(II), Ca(II) and Ga(III) was as high as 11.23 L/g, exhibiting a selectivity coefficient more than 3.5 times higher than that of competing ions and demonstrating superior selectivity. After five adsorption-desorption cycles, the capture rate was still greater than 87 %. DFT calculations indicated that the uptake of Ge(IV) by PPPs-CS might be due to the ion exchange and complexation between the catechol groups on the surface of the PPPs-CS and Ge(OH) 4 . The pomegranate peel polyphenols could effectively improve the diversity and chemistry of the active sites for Ge(IV) binding by chitosan and enhance the specific recognition performance for Ge(IV). Overall, PPPs-CS has significant advantages and potential as environmentally friendly and cost-effective materials for the recovery of Ge(IV) from wastewater.
研究领域:
环境科学
·
材料科学
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化学工程
·
废水处理
·
重金属回收
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Burns & Trauma
·
2025
·
IF 9.6
Yang Shuhao,Hu Qianshui,Hu Yingkun,Pu Zhengguang,Lan Yixuan,Wu Haoming,Zhu Gaohui,Gao Zhixiang,Yang Jianye,Tan Shuai,Hu Ning,Hu Xulin,Qin Leilei
BackgroundInfectious bone defects are characterized by persistent bacterial invasion and an immune microenvironment imbalance, which significantly hinders bone regeneration. Recently, numerous bone repair materials have been developed to address the complex pathological microenvironment associated with infectious bone defects. However, dynamic changes in the defect size after infectious debridement pose a significant challenge for achieving effective bone integration of artificial bone grafts.MethodsUsing low-temperature printing combined with the freeze-drying technology, a shape-memory scaffold with a biomimetic porous structure of cancellous bones was fabricated by compositing left-handed poly(L-lactic acid)-trimethylene carbonate (PLLA-TMC) with citric acid-modified hydroxyapatite (CHA). The scaffold (PT/CHA) was further coated with a metal-polyphenol network tannic acid-magnesium (TA-Mg) on its surface through the "mussel" effect, enabling the sequential treatment of infectious bone defects.ResultsThe scaffold can adaptively integrate with defect interfaces at the physiological temperature (37°C), achieving superior bone integration performance. The incorporation of citric-acid-modified hydroxyapatite effectively optimizes the polymer-inorganic phase printing ink system, significantly enhancing the mechanical strength and mineralization capacity of the scaffold. Meanwhile, the external tannic-acid-magnesium metal-polyphenol coating (TA-Mg) demonstrates excellent pathogen clearance properties both in vitro and in vivo. It also influences macrophage polarization to regulate the immune microenvironment, ultimately promoting bone regeneration in infectious bone defects.ConclusionsThe PT/CHA@TA-Mg scaffold achieves bone integration through adaptive filling and enables the multi-stage treatment of infectious bone defects via antibacterial, immune-regulatory and osteogenic differentiation.
研究领域:
生物医学工程
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组织工程
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骨再生
·
感染性疾病
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生物材料
·
免疫调节
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