Analytical Methods
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2018
·Vol. 10
·期 34
·4183-4188
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IF 2.073
Renjun Wang,Xiaolu Yan,Jing Sun,Xiao Wang,Xian-En Zhao,Wei Liu,Shuyun Zhu
In this contribution, DNA-templated silver nanoclusters (DNA-AgNCs) were successfully utilized as novel fluorescent probes for the detection of quinolones for the first time. The as-prepared DNA-AgNCs exhibited strong emission at 596 nm, which could be quenched by Cu2+ ions. The addition of quinolones caused restoration of fluorescence intensity of the DNA-AgNCs–Cu2+ system. This detection protocol exhibited excellent sensitivities for four kinds of quinolones. For instance, the linear ranges were from 20 to 100 nM for nalidixic acid with the limit of detection (LOD) 2.0 nM, from 5 to 120 nM for cinoxacin with the LOD 1.2 nM, from 5 to 100 nM for ciprofloxacin with the LOD 1.0 nM, and from 1 to 60 nM for moxifloxacin with the LOD 96 pM, respectively. Benefiting from high sensitivity, satisfactory recoveries were obtained for trace detection of quinolones in real samples, such as tablets and human urine.
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NEW JOURNAL OF CHEMISTRY
·
2018
·Vol. 42
·期 19
·15839-15846
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IF 3.201
Yue Wang,Qingtao Meng,Qian Han,Guangjie He,Yaoyun Hu,Huan Feng,Hongmin Jia,Run Zhang,Zhiqiang Zhang
Effective discrimination of cysteine (Cys) from biothiols and other amino acids has gained increasing attention due to the essential roles of this molecule in biological systems. In this work, we report a new strategy for the detection of Cys in aqueous media and live cells using a simple Cu2+ ensemble (L–Cu2+). A coumarin–salicylaldehyde hydrazone fluorescent ligand (L) was initially synthesized and well characterized. L features high affinity towards Cu2+ (Ka = 1.995 × 106 M−1), accompanied by effective quenching of fluorescence in HEPES buffer. The in situ generated chemosensing ensemble, L–Cu2+, is able to specifically respond to Cys over other biothiols and amino acids through a displacement approach. As a result, a remarkable recovery of fluorescence and UV-vis absorption spectra can be observed. L–Cu2+ showed high selectivity and sensitivity towards Cys. The detection limit is estimated to be 15 nM. Using L–Cu2+ as the fluorescent probe, imaging of Cys in live A549 cells was successfully demonstrated.
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TALANTA
·
2016
·
IF 4.035
Ying Chen,Ling Chen,Yidian Ou,Zhenhua Wang,Fengfu Fu,Liangqia Guo
A novel signal amplification strategy based on Cu 2+ -dependent DNAzyme was developed for sensing Cu 2+ ion by combining hybridization chain reaction (HCR) with fluorescence resonance energy transfer (FRET) technique. In the presence of Cu 2+ ion, the substrate strands of Cu 2+ -dependent DNAzyme immobilized on magnetic beads were specifically cleaved and released. The released strands initiated the HCR process of hairpin H1 and H2 labeled with FAM as the donor and TAMRA as the acceptor, respectively. Long nicked dsDNA structures were self-assembled to bring the donor and the acceptor in close proximity, resulting in a FRET process. The relative ratio of fluorescent intensities of the acceptor and donor was used to quantitatively detect Cu 2+ ion with a limit of detection of 0.5 nmol L −1 . This proposed biosensor was applied to detect Cu 2+ ion in tap water with satisfactory results.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2016
·
IF 4.758
Jia Tao,Qiang Zeng,Lishi Wang
Compared to visible light, near infrared (NIR) light is attractive for the development of novel assay method because it can provide deeper imaging penetration and lower fluorescence background and scattering intensity. In this paper, near infrared (NIR) quantum dots (QDs) were synthesized under a convenient and mild condition, afterwards applied to highly sensitive detection of Cu 2+ and monitoring the change of Cu 2+ concentration through in vitro and in vivo fluorescent imaging. The quenching of NIR light was resulted from the aggregation of NIR QDs induced by the competitive binding between 3-mercaptopropionic acid (MPA) and the Cu 2+ present in the solution. A low detection limit of 5 × 10 −8 M and a broad linear detection range from 1 × 10 −7 to 5 × 10 −5 M could be realized in this strategy for the quantitative evaluation of Cu 2+ . This NIR QDs based sensor possess high selectivity, rapid response and excellent photostability. Furthermore, the great potential of this NIR nanosensor has also been fully proved by the in vitro cellular imaging and in vivo imaging.
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JOURNAL OF HAZARDOUS MATERIALS
·
2017
·
IF 6.065
Junzhuo Liu,Jun Tang,Juanjuan Wan,Chenxi Wu,Bruce Graham,Philip G. Kerr,Yonghong Wu
Responses of microbial communities to nanotoxicity in aquatic ecosystems are largely unknown, particularly with respect to relationship between community dynamics and functions. Here, periphytic biofilms were selected as a model of species-rich microbial communities to elucidate their responses when exposed to titanium dioxide nanoparticles (TiO 2 -NPs). Especially, the relationships between the functions (e.g. organic matter and Cu 2+ removal) and community dynamics after long-term exposure to TiO 2 -NPs were assessed systematically. After 5 days exposure to TiO 2 -NPs (5 mg L −1 ), periphytic biofilms showed sustainable functions in pollutant removal and strong plasticity in defensing the toxic disturbance of TiO 2 -NPs, including photosynthesis and carbon metabolic diversity. The sustainable pollutant removal functions of periphytic biofilms were attributed to their functional redundancy. Specifically, periphytic biofilms altered their composition with cyanobacteria, Sphingobacteriia and Spirochaetes being the newly dominant taxa, and changed the carbon substrate utilization pattern to maintain high photosynthesis and metabolic rates. Moreover, extracellular polymeric substances (EPS) especially proteins were overproduced to bind the NPs and thereby reduce the nanotoxicity. The information obtained in this study may greatly help to understand the interactions between microbial community dynamics and function under NPs exposure conditions and functional redundancy is an important mechanism of periphytic biofilms to maintain sustainable functions.
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RSC Advances
·
2016
·Vol. 6
·期 83
·79641-79650
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IF 3.289
Ping Tan,Junjie Wen,Yongyou Hu,Xiaojun Tan
Novel poly(vinyl alcohol)/graphene oxide (PVA/GO) nanofibers were fabricated by electrospinning and applied to remove Cu2+ and Cd2+ from aqueous solution. The adsorption performances of the PVA/GO nanofibers were investigated by removing Cu2+ and Cd2+. The results showed that the adsorption of Cu2+ and Cd2+ onto PVA/GO nanofibers increased as the pH was increasing, but only slightly reduced with the increasing of ionic strength. The adsorption could reach equilibrium within 25 min and the experimental kinetic data followed the pseudo-second-order kinetic model. The equilibrium adsorption data can be well fitted with the Langmuir model. The thermodynamic parameters calculated from adsorption isotherms at four different temperatures indicated that the adsorption processes were endothermic and spontaneous. The PVA/GO nanofibers have good regeneration ability and can be recycled 8 times with a small amount of loss in adsorption efficiency. FTIR and XPS results indicated that the carboxyl and the carbonyl groups of GO on the surface of the nanofibers mainly participated in the adsorption of Cu2+ and Cd2+.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Yibing Yan,Fangxiang Song,Songye Li,Tingxian Li,Qian Li,Yan Li
Accurate pesticide application enhances efficiency, reduces usage and minimizes environmental risks. This study employed amino-functionalized degradable mesoporous organosilicon nanoparticles (DMON-N) as a carrier, with chitosan (CS) acting as a capping agent. Chemical cross-linking of CS with Cu 2+ through chelation promotes the formation of Cu 2+ -CS complexes. A pH/redox dual-responsive prochloraz (PRO) delivery system (Cu-CS@PRO-DMON, CCPD) was constructed and evaluated for its potential application in controlling rice sheath blight. The successful preparation of CCPD was confirmed through a series of physicochemical characterizations. The findings demonstrated that CCPD exhibited a high PRO loading capacity (29.09 %) and that PRO could be released from CCPD for an extended duration (up to 144 h) under the influence of acidity and glutathione promotion. Furthermore, CCPD demonstrated excellent wettability (measured contact angle: 63.52 ± 0.42°), adhesion (23.83 ± 2.59 mg/cm 2 ) and resistance to rainfall washout on rice leaves. CCPD demonstrated superior antimicrobial efficacy to prochloraz technical (PRO TC) material and prochloraz emulsion in water (PRO EW). The biosafety assessment revealed that CCPD exhibited minimal acute toxicity to zebrafish, no discernible toxicological effects on human bronchial epithelial cells and no notable impact on rice.
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MICROCHEMICAL JOURNAL
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2018
·
IF 2.746
Lei Liu,Mao-Rong Xie,Fang Fang,Zhi-Yong Wu
Paper-based analytical device (PAD) with image-based colorimetric detection is advantageous in low-cost, fast, simple and suitable for on-site rapid analysis. However, the detection capability is normally limited. In this paper, a new method was proposed that combined a complex reaction and electrokinetic stacking, and the sensitivity of PAD was effectively increased as demonstrated by the colorimetric detection of Cu 2+ with smartphone. A T-type paper fluidic channel was designed to realize the on-line complex reaction between polyethyleneimine (PEI) and Cu 2+ . Under electric field, positively charged PEI and Cu 2+ were driven through the two channels and eventually met on the same paper fluidic channel to form the positively charged complex of dark blue color. Meanwhile, the complex was stacked into a narrow and enhanced color band by field amplification effect. Under optimal experiment conditions, the band intensity was increased by three orders of magnitude compared with that of direct Cu 2+ imaging on the same paper substrate. A linear relationship in the range of 0.1–1.0 mM Cu 2+ was obtained (R 2 = 0.991), and a limit of detection of 30 μM was achieved, which is close to that by a desktop spectrophotometer. The selectivity of this method to other co-existing metal ions was also investigated. This work shows that by integration of a complex reaction and electro kinetic stacking, the sensitivity of image-based colorimetric detection can be greatly enhanced for rapid detection of Cu 2+ by PAD.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Hong Yan Zou,Qiang Wang,Tong Yang,Peng Fei Gao,Chun Mei Li,Yuan Fang Li,Cheng Zhi Huang
Synthesis and engineering of nanostructures with multiple components for the acceptable biosensing platform is a hot topic research. Herein, flower-like nonstoichiometric copper chalcogenides nanocrystals (Cu 2− x S y Se 1− y NCs) loaded with rhodamine 6G (R6G) were used as a selective and sensitive off-on fluorescent probe for biothiols based on inner filter effect (IFE). The Cu 2 −x S y Se 1− y showed the strong interband transition band in the UV–vis region and broad localized surface plasmon resonance (LSPR) in the near-infrared region which exhibited good light-harvesting features, and thus can show sufficient absorption of the excitation or emission light of fluorescent R6G with high fluorescence quenching ability. When the biothiols (glutathione, homocysteine and cysteine) were added, the interband transition bands of Cu 2− x S y Se 1− y NCs were suppressed, resulting in the fluorescence of R6G switched on. There was a good linear relationship between fluorescent recovery of R6G and the concentrations of glutathione, homocysteine and cysteine over the range of 1.0–150 μM, 1.0–200 μM, 1.0–150 μM with a detection limit of 32.0 nM, 34.0 nM and 31.0 nM, respectively. The established strategy exhibited the selective response to biothiol compounds and was also been applied for detecting biothiols in the human serum samples.
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FOOD CHEMISTRY
·
2024
·
IF 8.5
Huihui Li,Zhuangwei Zhang,Jin Chen,Mengchu Sun,Hongjin Tang
This study aimed to elucidate how Cu 2+ influences the interaction between catechins and zein using multi-spectral and molecular modeling techniques. As a result, UV–vis spectra revealed characteristic changes, indicating chelation between catechins and Cu 2+ at a 1:1 M ratio. Fluorescence spectra further confirmed interaction through a static quenching mechanism between catechins/catechin-Cu 2+ complexes and zein. Catechins induced changes in the microenvironment and hydrophobicity surrounding the binding site of zein, whereas Cu 2+ had minimal impact on these aspects. CD spectra underscored catechins' role in altering zein's secondary structure conformation, alongside Cu 2+ . Various types of interactions (hydrophobic, hydrogen bonding, electrostatic, and van der Waals) contributed to the binding of catechins/catechin-Cu 2+ complexes with zein. Molecular modeling elucidated key residues and binding conformations, highlighting the significance of hydrophobic interactions and hydrogen bonding in their association. These findings not only deepen our understanding of catechin-Cu 2+ -zein interactions but also underscore their potential applications in the food industry.
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CARBON
·
2024
·
IF 10.5
Lina Bao,Wanchun Luo,Qinying Li,Yu Zhang,Zitong Zhang,Xi Li,Le Wang,Jintao Zhang,Kun Huang,Xu Yu,Li Xu
Metal dyshomeostasis is one of the pathological features of Alzheimer's disease (AD). Cu 2+ is believed to induce the formation of neurotoxic β-amyloid (Aβ) aggregates and aggravate oxidative damage. It is challenging to take effective therapeutic interventions towards the complicated pathogenesis related to Cu 2+ . Herein, chiral carbon dots were proposed for Cu 2+ -related AD therapy. The chiral CDs were prepared based on the ternary carbon sources, using L/ d -cysteine as the chiral carbon source, citric acid as the carbonization source and β-cyclodextrin as the chirality inducer, reserving and enhancing the chirality of CDs. Compared to L-Cys-CDs, D-Cys-CDs exhibited enhanced chirality, stronger affinity towards Cu 2+ and comparable antioxidant ability, but better inhibition on peroxidase-like activity of Cu 2+ or Aβ 42 -Cu 2+ . Thus, D-Cys-CDs performed better to inhibit Cu 2+ -induced Aβ aggregation. Meanwhile, D-Cys-CDs can alleviate the cytotoxicity of Aβ 42 -Cu 2+ with satisfactory anti-inflammation effect and scavenging ability towards intracellular reactive oxygen species. D-Cys-CDs succeeded in reducing Aβ deposits and releasing paralysis for the AD model CL2120 nematodes. D-Cys-CDs with an enantiomeric effect on Cu 2+ -induced Aβ aggregation and the peroxidase-like activity of Cu 2+ was promising as the multifunctional therapeutic agent for Cu 2+ -induced AD therapy.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Hong Yang,Shanshan Zhu,Lihua Pang,Yifei Ma,Xiaoxue Liu,Jinhua Liu
ABSTRACT Using a single emission-wavelength to accurately/sensitively quantify disease markers in complex environments still faces enormous challenges. In this study, we construct a Cu 2+ -triggered wavelength-tunable fluorescent immunoassay for real-time detection of cTnI in human serum. The developed platform is based on an “on-off-on” strategy, which involves the rapid in-situ fluorogenic reaction of dopamine (DA) and phenol analogue by Cu 2+ , strong coupling between PPi and Cu 2+ as well as the specific hydrolysis of PPi into orthophosphate by alkaline phosphatase. By using cTnI as a model antigen, the assay shows good linearity relationships between the fluorescence response and cTnI concentration. Furthermore, we used different phenol analogues (1,3-dihydroxynaphthalene, 8-hydroxyjulolidine, 1,5-dihydroxynaphthalene) as fluorescence reaction substrates to achieve multi-channel (blue, green and yellow) real-time monitoring of cTnI with detection limits of 0.17 ng/mL, 0.17 ng/mL and 0.33 ng/mL, respectively. We successfully applied the wavelength-tunable ELISA to detect cTnI levels in real human serum, and the results were compared with a commercial ELISA with satisfactory outcomes. Therefore, our proposed platform demonstrates great potential in the early diagnosis of acute myocardial infarction.
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