Acta Cirurgica Brasileira
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2018
·Vol. 33
·期 3
·207-215
·
IF 0.933
Wu Jing,Pan Lin,Jin Xueqin,Li Weihua,Li Hongbing,Chen Jianmao,Yang Wen
Purpose: To investigate whether oxymatrine (OMT) prevents hepatic fibrosis in rats by regulating liver transforming growth factor β1 (TGF-β1) level. Methods: Hepatic fibrosis was induced in rats by thioacetamide (TAA). Blood was collected at the end of week 12 to determine the levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and glutathione (GSH). Changes in liver tissue were observed after hematoxylin-eosin (HE) staining. Results: Fibrosis was confirmed by Masson’s collagen staining. Liver TGF-β1 level was determined by ELISA. OMT significantly reduced serum ALT and AST but increased GSH levels in rats with hepatic fibrosis. Moreover, it significantly improved liver histology in rats with TAA-induced hepatic fibrosis. It significantly decreased liver TGF-β1 level compared to that in the untreated group. It also significantly reduced collagen deposition in rats. Conclusion: Oxymatrine is effective in protecting rats from thioacetamide-induced hepatic fibrosis by regulating TGF-β1 expression.
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ENVIRONMENTAL MONITORING AND ASSESSMENT
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2018
·Vol. 190
·期 2
·1-13
·
IF 1.804
He Xiaomin,Li Aimin,Wang Shengyao,Chen Hao,Yang Zixin
The occurrence of eight perfluorinated compounds (PFCs) in the surface sediments from 10 sampling sites spread across the Danjiangkou Reservoir was investigated by isotope dilution ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) after solid-phase extraction (SPE). All the sediments from the 10 sites contained detectable levels of PFCs. The total concentration of the target PFCs in each sediment sample ( C ∑PFCs ) ranged from 0.270 to 0.395 ng g −1 of dry weight, and the mean value of C ∑PFCs was 0.324 ± 0.045 ng g −1 of dry weight for the whole reservoir. For each perfluorinated compound in one sediment, perfluorooctane sulfonate (PFOS) or perfluoro- n -butanoic acid (PFBA) consistently had a higher concentration than the other six PFCs, while perfluoro- n -octanoic acid (PFOA) was always undetectable. In terms of spatial distribution, the total and individual concentrations of PFCs in sediment from downstream sites of the Danjiangkou Reservoir were higher than those from upstream sites. Factor analysis revealed that PFCs in the sediment samples originated from electroplating and anti-fog agents in industry, food/pharmaceutical packaging and the water/oil repellent paper coating, and the deposition process. The quotient method was utilized to assess the ecological risk of PFCs in the sediments of the Danjiangkou Reservoir, which showed that the concentrations of PFCs were not considered a risk. In this study, detailed information on the concentration level and distribution of PFCs in the sediments of the Danjiangkou Reservoir, which is the source of water for the Middle Route Project of the South-to-North Water Transfer Scheme in China, was reported and analyzed for the first time. These results can provide valuable information for water resource management and pollution control in the Danjiangkou Reservoir.
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RSC Advances
·
2019
·Vol. 9
·期 26
·14708-14717
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IF 3.049
Hongmei Yang,Miao Wang,Yihe Huang,Qiaoyu Qiao,Chunjie Zhao,Min Zhao
Mitomycin C (MMC), naturally synthesized by Streptomyces caespitosus, is a potent antineoplastic antibiotic for the treatment of various solid tumors. However, the defects of conventional MMC injections have greatly limited its clinical application due to its toxic side effects and non-specific interactions. To solve this problem, the PEG2k-Fmoc-Ibuprofen (PEG-FIbu) micellar nanocarrier was synthesized and the MMC-loaded micelles (PEG-FIbu/MMC) were prepared by thin film hydration method and characterized. Ibuprofen was used as a hydrophobic domain of PEG-FIbu nanocarrier, and we expect it to synergize with codelivered MMC in the overall antitumor activity. The in vitro release of PEG-FIbu/MMC was examined by dialysis method using MMC injection as a control. Our data suggested that PEG-FIbu/MMC micelles presented appropriate particle size, low CMC value, good stability, high drug loading efficiency and sustained release properties. In vitro cytotoxicity studies with several tumor cell lines showed that the carrier was effective in mediating intracellular delivery of MMC to tumor cells. In vivo pharmacokinetics, tissue distribution and therapeutic study proved that PEG-FIbu/MMC micelles prolonged blood circulation, significantly improved the tumor accumulation and therapeutic efficacy, and reduced undesirable side effect on normal tissues compared to MMC injection. In general, PEG-FIbu/MMC micelles represented an effective strategy to improve the performance for the delivery of MMC and safety of medication.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2017
·
IF 3.154
Xueke Liu,Peng Wang,Chang Liu,Yiran Liang,Zhiqiang Zhou,Donghui Liu
Beta-cypermethrin (beta-CP), an important pyrethroid insecticide, and its main acid metabolites are frequently detected in human samples. Because beta-CP may pose some risk to human health, we studied dynamics and residues of beta-CP and its metabolites in hen egg, droppings, blood, and 15 other tissues after continuous exposure. A digestive model was then used to study beta-CP’s digestive fate. Beta-CP and its metabolites significantly accumulated in tissues with high lipid contents and were readily transferred to eggs. Beta-CP was mainly metabolized into acid metabolites that accumulated in egg and edible tissues of laying hens, suggesting that humans may be exposed to beta-CP acid metabolites through food.
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ACS Applied Materials & Interfaces
·
2024
·
IF 8.3
Xue Long,Yuhua Xie,Qing Li,Shiao Zhu,Yazhou Chen,Fang Luo,Zehui Yang
The realization of a rechargeable zinc-air battery (ZAB) is hindered by the low intrinsic oxygen evolution reaction (OER) and oxygen reduction reaction (ORR) activities. In this work, an abundant built-in electric field is noticed in a 1D/2D CoO/CoS2 heterostructure, triggering electron transfer from CoO to CoS2 associated with a downshifted d band center of the Co atom mitigating the strong electrochemical adsorption of *OH species on active sites; thereby, boosted OER and ORR performance are achieved. Namely, the OER specific activity of CoO/CoS2 is enhanced by 3.8- and 2.2-fold compared to the counterpart of CoO and CoS2, respectively. Furthermore, the kinetic current density of CoO/CoS2, a fingerprint of intrinsic ORR activity, is promoted by 46 and 6.6 times relative to CoO and CoS2. The rechargeable ZAB performance attains 215.6 mW cm–2, 1.6-times better than Pt/C-IrO2. Moreover, the superior performance remained for 600 h. Besides, the battery performance of the all-solid-state ZAB reaches 83.8 mW cm–2, revealing its promising application in wearable device.
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Applied Sciences-Basel
·
2024
·Vol. 14
·期 20
·9193
·
IF 2.5
Xinyue Zeng,Zheng Zhu,Wei Chang,Bin Wu,Wei Huang
Biological composites such as bone, nacre, and teeth show excellent mechanical efficiency because of the incorporation of biominerals into the organic matrix at the nanoscale, leading to hierarchical composite structures. Adding a large volume of ceramic nanoparticles into an organic molecular network uniformly has been a challenge in engineering applications. However, in natural organisms, biominerals grow inside organic fibers, such as chitin and collagen, forming perfect ceramic/polymer composites spontaneously via biomineralization processes. Inspired from these processes, the in situ growth of calcium carbonate nanoparticles inside the chitosan network to form ceramic composites was proposed in the current work. The crystal growth of CaCO3nanoparticles in the chitosan matrix as a function of time was investigated. A weight percentage of ~35 wt% CaCO3composite was realized, resembling the high weight percentage of mineral phase in bones. Scanning and transmission electron microscopy indicated the integration of CaCO3nanocrystals with chitosan macromolecules. By growing CaCO3minerals inside the chitosan matrix, the elastic modulus and tensile strength increases by ~110% and ~90%, respectively. The in situ crystal growth strategy was also demonstrated in organic frameworks prepared via 3D printing, indicating the potential of fabricating ceramic/polymer composites with complicated structures, and further applications in tissue engineering.
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Nature Nanotechnology
·
2015
·Vol. 10
·期 7
·608-613
·
IF 34.048
Li Likai,Ye Guo Jun,Tran Vy,Fei Ruixiang,Chen Guorui,Wang Huichao,Wang Jian,Watanabe Kenji,Taniguchi Takashi,Yang Li,Chen Xian Hui,Zhang Yuanbo
For decades, two-dimensional electron gases (2DEG) have allowed important experimental discoveries1,2 and conceptual developments in condensed-matter physics3. When combined with the unique electronic properties of two-dimensional crystals, they allow rich physical phenomena to be probed at the quantum level4,5. Here, we create a 2DEG in black phosphorus—a recently added member of the two-dimensional atomic crystal family6,7,8—using a gate electric field. The black phosphorus film hosting the 2DEG is placed on a hexagonal boron nitride substrate. The resulting high carrier mobility in the 2DEG allows the observation of quantum oscillations. The temperature and magnetic field dependence of these oscillations yields crucial information about the system, such as cyclotron mass and lifetime of its charge carriers. Our results, coupled with the fact that black phosphorus possesses anisotropic energy bands with a tunable, direct bandgap6,7,8,9,10,11,12,13,14,15, distinguish black phosphorus 2DEG as a system with unique electronic and optoelectronic properties.
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DRUG DELIVERY
·
2016
·
IF 4.843
Guolian Ren,Dan Liu,Weiling Guo,Menglin Wang,Chunnuan Wu,Mengran Guo,Xiaoyu Ai,Yongjun Wang,Zhonggui He
There is a strong desire to develop docetaxel (DTX) formulation with good therapeutic effectiveness in view of serious adverse reactions of the commercial formulation of DTX (Taxotere®). In this study, a redox-responsive DTX-vitamin E prodrug was successfully formulated into liposomes with the drug loading of 4.14% ± 0.10%. Compared with DTX liposomes, the DTX prodrug liposomes (DPLs) showed good stability for 30-d shelf life and during dilution with different media. In vitro antitumor activity of DPLs on human prostatic carcinoma PC-3 cells and human lung cancer A549 cells was evaluated using cytotoxicity and apoptosis assays. In spite of a decrease in in vitro antitumor activity, the in vivo pharmacokinetic study reveals that DPLs exhibit significantly longer DTX plasma half-life (t1/2, 1.38-fold) and higher bioavailability (AUC0−t, 14.49-fold) compared with DTX liposomes. The antitumor activity of DPLs to the A549 tumor xenograft model showed selective accumulation in tumor tissue, significant inhibition the growth of the tumors and a much lower toxicity as seen in body weight loss, compared with DTX-Solution. Taken together, the results showed that DPLs is a promising strategy for DTX antitumor delivery.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2016
·
IF 3.138
Ziru Lian,Rong Pan,Jiangtao Wang
Norfloxacin chitosan/chitosan oligosaccharide microcapsules (NCCM) were prepared by emulsion-chemical crosslinking method. The characteristics of obtained microcapsules were evaluated by scanning electron microscopy, Fourier transform infrared spectroscopy and release experiments. Cumulative release profile of norfloxacin from the chitosan microcapsules in natural seawater was measured and the controlled release of drugs was at a uniform rate in 48 h. The chitosan microcapsules were applied onto the antibacterial study of the shrimp culture in natural seawater. It is observed that the seawater in the NCCM added groups was relatively clear and the biomass of Vibrio increased slowly in contrast to the control and norfloxacin groups. The inhibition rate of Vibrio in norfloxacin groups obvioursly decreased after the 5 th day, whereas, it remained high and stable during experiment period in NCCM groups. The results showed that the chitosan microcapsules as release materials have excellent antibacterial effects on Vibrio in the farming of Penaeus vannamei Boone. The controlled release could obviously reduce dosage of antibiotics and delivery times, and effectively improve the utilization rate of norfloxacin drugs for shrimps
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ACS Applied Materials & Interfaces
·
2016
·
IF 7.145
Donghui Liang,Zhong Lu,Hao Yang,Jingting Gao,Rong Chen
A novel silver nanoparticles (AgNPs)/chitosan composite dressing with asymmetric wettability surfaces was successfully prepared via a simple two-step method for biomedical applications as wound healing materials. First, AgNPs were assembled into the chitosan sponge which was prepared by lyophilization process. Then one side of the sponge was modified by a thin layer of stearic acid. The incorporation of AgNPs into chitosan dressing could enhance the antibacterial activity against drug-sensitive and drug-resistant pathogenic bacteria. The asymmetric surface modification endows the dressing with both highly hydrophobic property and inherent hydrophilic nature of chitosan. The hydrophobic surface of the dressing shows waterproof and antiadhesion for contaminant properties, whereas the hydrophilic surface preserves its water-absorbing capability and efficiently inhibits the growth of bacteria. Furthermore, the AgNPs/chitosan composite dressing displays improved moisture retention and blood clotting ability compared to the unmodified dressings. Cytocompatibility test evaluated in vitro and in a wound infection model illustrates the nontoxic nature of the composite dressing. More importantly, the in vivo wound healing model evaluation in mice reveals that the asymmetric AgNPs/chitosan dressing promotes the wound healing and accelerates the reepithelialization and collagen deposition. The silver accumulation in mice body treated by the composite dressing is far lower than that of the clinically used Acasin nanosilver dressing treated mice. This work indicates the huge potential of the novel AgNPs/chitosan wound dressing with asymmetrical wettability for clinical use.
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Journal of the Taiwan Institute of Chemical Engineers
·
2016
·
IF 2.848
Xiao-Wen Zhu,Min-Ping Wei,De-Ping Xu,Ya-Hui Guo,Yun-Fei Xie,Wei-Rong Yao
This study evaluated the antitumor effect of some fractions extracted from Sapindus spp. on breast cancer. Four water-extracted fractions (I, II, III, IV) were isolated from the pericarp of Sapindus spp. using X-5 and AB-8 macroporous resin columns and MCI gel column. The in vitro effect was determined using the MTT assay. Our results indicated that fractions I-IV did not exhibit significant cytotoxic effects towards the normal cell MDA-kb2; Fraction II and IV inhibited the growth of MCF-7 (estrogen receptor positive), MDA-MB-231 (estrogen receptor negative), and MDA-MB-435 s human breast cancer cell lines; of these, MDA-MB-435 s cell line was the most sensitive, and the IC 50 of Fraction II and IV against this cell line were 33.11 and 30.06 µg/mL, respectively. In the in vivo experiment, Fraction II and IV showed a strong antitumor effect at a dose of 162.5 mg/kg/d, with inhibition rate of 40.43% and 38.77%. Fraction II and IV also induced apoptosis of tumor cells, activations of Bax (Fraction II) and inhibition of Bcl-2 (Fraction II and IV). Our results underscore the pharmacological potential of Sapindus spp. extracts.
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Scientific Reports
·
2017
·Vol. 7
·期 1
·1-8
·
IF 4.259
Zhang Xiaoping,Chingin Konstantin,Zhong Dacai,Liang Juchao,Ouyang Yongzhong,Chen Huanwen
1-Pyrroline has a highly characteristic odor, which is employed by living organisms for chemical signaling and other purposes, but the mechanism whereby this odor is formed remains poorly understood. Here we used a combination of ambient mass spectrometry (AMS) and nuclear magnetic resonance (NMR) spectroscopy to experimentally address the mechanistic aspects of 1-pyrroline volatility and other controversies regarding the chemistry of this compound. Our results indicate that in solution the volatility of the monomer species is significantly higher than that of the trimer species, and 1-pyrroline is evaporated mainly in its monomer state. Neat 1-pyrroline is essentially the pure trimer and displays ca. 100-fold lower evaporation rate than the monomer state in solution. In the gas-phase the trimer species is irreversibly decomposed into monomer species. Under equilibrium conditions the vapor of 1-pyrroline entirely consists of monomer species. The evaporation rate of 1-pyrroline in water has a step-wise dependence on the solution pH, the abrupt increase in volatility (>1,000-fold) occurring around the pKa value of 1-pyrroline (6.8). The pronounced step-wise dependence of 1-pyrroline volatility around neutral pH may also be an important evolutionary factor allowing living systems to regulate the odor strength from very weak to very strong with minimal efforts.
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