ACS Applied Bio Materials
·
2023
·
IF 4.7
Wen-Xing Zhang,Ze-Lei Zhou,Qi-Yan Lv,Xiejie Song,Junyang Chen,Chang-Bin Niu,Hui-Fang Cui
Cancer starvation/photothermal combined tumor therapy (CST/PTT) has attracted great interest attributed to their mutual compensation and synergistically enhanced effect. However, the very low O2 supply in the tumor microenvironment (TME) greatly limits the CST efficiency of glucose oxidase (GOx). Additionally, the easy degradation in blood circulation and significant off-target effects are big challenges for clinical applications of the GOx-based CST. In this study, a drug delivery system (DDS) with specific tumor-targeted GOx delivery, near-infrared (NIR) light and TME responsive O2 generation, NIR-responsive glucose consumption, high GOx loading, and efficient NIR photothermia was developed. Positively charged AuNRs@MnO2@SiO2 nanoparticles (named AMS+ NPs) were synthesized. GOx was covalently loaded with a high loading ratio of 36.0%. Finally, a thermosensitive biomimetic hybrid membrane composed of a thermosensitive lipid (TSL) membrane, red blood cell membrane (RBCM), and 4T1 cancer cell membrane (CCM) was coated on the NPs through a double-layer strategy. The AMS+-G@TSL@[RBC-CC-TSL]M NPs consumed 32.7 times glucose at 50 °C as that at 37 °C and generated 4.9 times O2 upon NIR laser irradiation. The thermosensitive biomimetic NPs showed an efficient targeting capability to the homotypic 4T1 cancer cells/tumors accompanied by good biocompatibility, macrophage evading capability, high cancer cell cytotoxicity, and excellent antitumor efficacy. The tumor growth inhibition ratio with NIR laser irradiation reached 92.8%. The AMS+-GOx@TSL@[RBC-CC-TSL]M NPs provide a smart, efficient, safe, PTT/CST combined DDS for highly efficient tumor therapy.
研究领域:
生物医学工程
·
纳米技术
·
癌症治疗
·
药物递送系统
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2022
·
IF 8.008
Zizhong Yang,Birong Liu,Ting Huang,Bao-Ping Xie,Wen-Jun Duan,Min-Min Li,Jin-Xiang Chen,Jun Chen,Zong Dai
Sensitive and specific imaging of microRNA (miRNA) in living cells is of great value for disease diagnosis and monitoring. Hybridization chain reaction (HCR) and DNAzyme-based methods have been considered as powerful tools for miRNA detection, with low efficient intracellular delivery and limited amplification efficiency. Herein, we propose a Hairpins@MnO2 nanosystem for intracellular enzyme-free exponential amplification for miRNA imaging. The enzyme-free exponential amplification is based on the synergistic cross-activation between HCR and DNAzymes. The MnO2 nanosheets were employed as the carrier of three kinds of hairpin DNA probes and further provided appropriate Mn2+ as DNAzyme cofactors in the living cell. Upon entering cells and in the presence of highly expressed glutathione (GSH) in tumors, MnO2 is reduced to release Mn2+ and the three kinds of hairpin DNA probes. In the presence of target miRNA, the released hairpin DNA H1 and H2 probes self-assemble via HCR into the wire-shaped active Mn2+-based DNAzymes which further catalyze the cleavage of H3 to generate numerous new triggers to reversely stimulate HCR amplifiers, thus offering tremendously amplified Förster resonance energy transfer readout. The method has a detection limit of 33 fM, which is 2.4 × 104 times lower than that of the traditional HCR system. The developed method also has a high specificity; even miRNAs with a single base difference can be distinguished. Live cell imaging experiments confirmed that this Hairpins@MnO2 nanosystem allows accurate differentiation of miRNA expression of cancer cells and normal cells. The method holds great potential in biological research of nucleic acids.
研究领域:
生物化学
·
分子生物学
·
纳米技术
·
癌症诊断
✓ 已验证
DOI
PubMed
高影响因子
TALANTA
·
2022
·
IF 6.556
Jia Liu,Rui Ma,Wei Ha,Hai-Xia Zhang,Yan-Ping Shi
The inhibition of acetylcholinesterase (AChE) from decomposing acetylcholine (ACh) is regarded as a common therapeutic strategy for Alzheimer's disease (AD). Screening AChE inhibitors is of great significance for AD treatment . In this work, AChE was physically absorbed on the synthesized MnO 2 -ZIF-67 through electrostatic interaction for the first time, and the fabricated MnO 2 -ZIF-67 immobilized AChE coupled with capillary electrophoresis (CE) was applied to screening AChE inhibitor. The established MnO 2 -ZIF-67 immobilized AChE with excellent pH, thermal and organic solvents tolerance stability was evaluated by Ellman's method. Huperzine A was used as model inhibitor to investigate the inhibition kinetics of the immobilized AChE, which possess the feasibility and applicability for its inhibitor screening. The screening mechanism of AChE inhibitor was based on the Ellman's method and measured by the changes of 5-thio-2-nitrobenzoic acid (TNB), which was produced by the reaction of thiocholine (TCh) with 5,5-dithiobis-(2-nitrobenzoic acid) (DTNB). Benefiting from the advantages of convenience and high sensitivity, the proposed platform was employed to screen AChE inhibitors from 11 compounds isolated from Inula macrophylla , and macrophyllic acid A (compound 1 ), dibutyl phthalate (compound 2 ) exhibited strong AChE inhibitory activity. Thus, this work not only provides a promising platform for monitoring AChE activity, but also holds great potential for the further exploration of active components with AChE inhibitory activity from Inula macrophylla .
研究领域:
药学
·
分析化学
·
神经科学
·
药理学
·
植物化学
✓ 已验证
产品关联
DOI
高影响因子
ANALYST
·
2018
·Vol. 143
·期 23
·5764-5770
·
IF 3.864
Chen Men,Chun Hong Li,Xue Mei Wei,Jia Jun Liu,Yu Xin Liu,Cheng Zhi Huang,Shu Jun Zhen
Ricin is an extremely potent ribosome-inactivating protein and serves as a likely food biocontaminant or biological weapon. Thus, simple, sensitive and accurate analytical assays capable of detecting ricin are urgently needed to be established. Herein, we present a novel method for ricin B-chain (RTB) detection by using two materials: (a) a highly efficient hybrid probe that was formed by linking a glucose oxidase (GOD)-encapsulated liposome (GOD-L) to magnetic beads (MBs) through hybridization between an aptamer and a blocker and (b) a new low-background g-C3N4–MnO2 sandwich nanocomposite that exhibits fluorescence resonance energy transfer (FRET) between the g-C3N4 nanosheet and MnO2. In the presence of RTB, the strong binding between RTB and the aptamer can release the blocker-linked liposome from the surface of the MBs. After magnetic separation, the decomposed liposome can release GOD to catalyze the oxidation of glucose, generating a certain amount of H2O2. Then, H2O2 can reduce MnO2 of the g-C3N4–MnO2 nanocomposite to Mn2+, which leads to the elimination of FRET. Thus, the fluorescence of the g-C3N4 nanosheet will be turned on. Because of the excellent signal amplification ability of liposome and the characteristic highly sensitive response of the g-C3N4–MnO2 nanocomposite toward H2O2, RTB could be detected sensitively based on the significantly enhanced fluorescent intensity. The linear range of detection was from 0.25 μg mL−1 to 50 μg mL−1 and the limit of detection (LOD) was 190 ng mL−1. Moreover, the proposed assay was successfully applied in the detection of the entire ricin toxin content in a castor seed.
研究领域:
分析化学
·
生物化学
·
毒理学
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Biomaterials Science
·
2023
·Vol. 11
·期 6
·2243-2252
·
IF 6.6
Anni Cui,Ying Bao,Haitao Xu,Xin Mu,Xiahua Zhong,Wynn Wee,Fanqi Wu,Guiye Shan
The rapid emergence of drug-resistant bacteria has attracted great attention to exploring advanced antibacterial methods. However, single-modal antibacterial therapy cannot easily eliminate drug-resistant bacteria completely due to its low efficacy. Therefore, it is essential to achieve multi-modal antibacterial therapy effectively. Herein, a dual-modal ROS generator was designed based on photosensitive PDA–MnO2@Ce6/liposome (PMCL) nanozymes for synergistic chemo-photodynamic therapy. PMCL nanozymes adhere to bacteria through liposome–membrane fusion. Meanwhile, PMCL catalyzes endogenous hydrogen peroxide (H2O2) to generate hydroxyl radicals (˙OH) and singlet oxygen (1O2) under laser irradiation. Furthermore, the photothermal effect can accelerate the generation of ROS. Based on dual-enzyme activities (mimicking peroxidase and catalase) and photodynamic properties, PMCL achieves powerful antibacterial efficacy and mature bacterial biofilm eradication. With the synergistic chemo-photodynamic effects, bacterial populations decrease by >99.76% against Gram-positive S. aureus and Gram-negative E. coli. Notably, the synergistic antibacterial properties of PMCL nanozymes are further explored using a mouse wound model of S. aureus infection. This work fabricated an efficient dual-modal ROS generator to kill bacteria, further providing a new strategy for treating wound infection.
研究领域:
生物医学工程
·
纳米技术
·
抗菌治疗
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2024
·
IF 5.6
Tengteng Wang,Lian Xue,Chao Di,Peng Ma,Xiaojun Feng,Peng Chen,Wei Du,Bi-Feng Liu
Heparin, a widely studied glycosaminoglycan, plays crucial roles in the regulation of various physiological and pathological processes. Therefore, it's important to develop highly selective and sensitive methods for convenient monitoring of heparin levels in biological systems. We report the design and synthesis of Fe 3 O 4 @PDA@MnO 2 nanoparticles (FPM-NPs), which exhibit dual enzymatic activities, enabling quantitative detection of heparin. The FPM-NPs feature a unique tri-layer spherical shell structure, possessing both peroxidase-like and oxidase-like activities, and catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence or absence of H 2 O 2 . Remarkably, upon co-incubated with heparin, the oxidase activity of FPM-NPs decreases, while the peroxidase activity increases. By leveraging these dual enzymatic properties of FPM-NPs, a highly sensitive and specific colorimetric detection of heparin is achieved, with a detection limit reaching 6.51 nM and a good linear response to quantify heparin ranging 10–800 nM. Additionally, the developed FPM-NPs are successfully applied to measure heparin in fetal bovine serum samples. We also extend this detection method to a paper-based chip, enabling portable detection of heparin through grayscale analysis of mobile phone photographs. The multi-nanozyme-based heparin detection approach provides a new perspective for future research on expanding the application of nanocomposite materials in biomedical detection and analysis.
研究领域:
纳米技术
·
生物医学工程
·
分析化学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nanoscale
·
2021
·Vol. 13
·期 39
·16571-16588
·
IF 7.79
Yu Zhang,Yifeng Yang,Jinsheng Shi,Lili Wang
Weak acidity (6.5–6.9) and limited H2O2 level in the tumor microenvironment (TME) usually impact the therapeutic effect of chemodynamic therapy (CDT) for cancer. A Specific TME promotes the formation of an immunosuppressive microenvironment and results in high rate of recurrence and metastasis of cancer. Fe3O4@ZIF-8/GOx@MnO2 multi-layer core shell nanostructure was constructed as a hybrid nanozyme. After magnetic targeting of the tumor site, the outermost MnO2 shell catalyzed H2O2 in TME to produce O2 and was broken due to the reaction with glutathione. Due to the acid response, the ZIF-8 layer would crack and release glucose oxidase (GOx) and Fe3O4. The generated O2 was utilized by GOx in starvation therapy to consume glucose and produce H2O2 and gluconic acid. The Fenton reaction efficiency of Fe(II) was improved by the increased H2O2 concentration and the enhanced acidity in TME. At the same time, the intrinsic photothermal effect of Fe3O4 upon 808 nm laser irradiation promoted the activity of MnO2 and GOx as oxidase, and Fe(II) as catalase-like, and ablated the primary tumor. Moreover, the hybrid nanozyme can facilitate the transformation of M2-type macrophages to M1-type, and strong systemic antitumor immune effect was induced. A synergy of multiple therapeutic modes including starvation therapy, CDT, photothermal therapy (PTT), and immunotherapy can be realized in the hybrid nanozyme for tumor therapy.
研究领域:
纳米技术
·
癌症治疗
·
生物医学工程
·
材料科学
·
免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LANGMUIR
·
2020
·
IF 3.557
Ziyi Shui,Xiangbiao Liao,Yuan Lei,Jia Ni,Yilun Liu,Yong Dan,Wei Zhao,Xi Chen
Aluminum–air batteries possess high theoretical specific capacities and energy densities. However, the desired application performance in the field of flexible electronics is limited by the rigid battery structure and slow kinetics of the oxygen reduction reaction (ORR). To address these issues, flexible, stretchable, and customizable aluminum–air batteries with a reference to honeycomb shape are composed of multilayer single battery units to achieve large scalability and start–stop control. The single aluminum–air battery combines MnO2 with N/S codoped graphene to improve the electrocatalytic activity. Benefiting from an efficient electrocatalyst and reasonable structural design, the single aluminum–air battery exhibits excellent electrochemical characteristics under deformation conditions with a high specific capacity and energy density (1203.2 mAh g–1 Al and 1630.1 mWh g–1 Al). Furthermore, the obtained honeycomb-shaped stretchable aluminum–air batteries maintain a stable output voltage over the 2500% stretching. More interestingly, the stretchable honeycomb structure not only can solve the start–stop control problem but also has the potential to reduce the self-corrosion in disposable metal–air batteries. In addition, owing to the customizable shapes and sizes, the honeycomb-shaped stretchable aluminum–air batteries facilitate the integrated application of flexible batteries in wearables.
研究领域:
材料科学
·
电化学
·
柔性电子
·
能源存储
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF ALLOYS AND COMPOUNDS
·
2022
·
IF 6.371
Yulai Zhao,Zhongzheng Zhu,Anjun Wang,Longqiang Xiao,Linxi Hou
Porous carbon materials have been widely used as electrode materials for supercapacitors . However, low specific capacitance severely hinders the practical application. To further improve the capacitance, this work reports a facile approach to prepare N, S co-doped hierarchical porous carbon/MnO 2 composites (NSPC/MnO 2 ). Acidified thiourea, acting as N and S source, is used to crosslink sodium alginate (SA) to give SA-based composite gels which are pyrolyzed to produce NSPC/MnO 2 . X-ray diffraction and X-ray photoelectron spectra proved that manganese exists in the porous carbon in the form of MnO 2 . The obtained NSPC/MnO 2 exhibit hierarchical porous structure, remarkable mesoporous feature (the proportion of mesopores and macropores reached 32.69%), and high specific surface area of 1701 m 2 g −1 . Meanwhile, NSPC/MnO 2 show abundant heteroatom doping, i.e. Mn (2.05 at%), N (6.76 at%), and S (2.16 at%), which provided plenty of pseudocapacitance. The electrochemical tests show that NSPC/MnO 2 as electrode material can reach a high specific capacity of 281.1 F g −1 at 1 A g −1 (which is almost 1.85 times higher than the comparative sample), with good cycling stability (86.02% after 5000 cycles at 10 A g −1 ).
研究领域:
材料科学
·
电化学
·
能源存储
✓ 已验证
产品关联
DOI
高影响因子
Biomaterials Science
·
2022
·Vol. 10
·期 5
·1317-1325
·
IF 7.59
Bengao Zhu,Xuefei Lv,Mengmeng Zhang,Huiying Wang,Senbin Chen,Jintao Zhu
Integrating magnetic resonance imaging (MRI)-targeted diagnosis with synergistic cascade treatments, such as chemo/chemodynamic therapy (CT/CDT), is highly desired for promoting the antitumor performance; however, the rational design of such “all-in-one” nanomedicine is still in its infancy. In this report, using MnO2 coated layered dihydroxide (LDH) as a carrier to load chemotherapy molecule 5-flurouracil (5-FU), a novel tumor microenvironment (TME) regulating nanodrug is formed: LDH/5-FU@MnO2. Combined guidance of CT/CDT and MRI is used to realize synergistic diagnosis and enhanced anti-tumor efficacy. MnO2 is converted into Mn2+ in the presence of reducing agent GSH, the in situ generated Mn2+, not only serves as the chemical fuel for the Fenton reaction, combining H2O2 depletion and ˙OH generation, but can also be used as a nuclear magnetic contrast agent for MRI. Moreover, the tumor acidic environment is able to trigger 5-FU release for initiating chemotherapy in the tumor zone. This “all-in-one” LDH/5-FU@MnO2 nanomedicine integrating multiple treatment modalities and magnetic resonance imaging properties, causes persistent modulation of the TME and exhibits effective antitumor theranostic performance. Such a sophisticated nanomedicine design not only achieves improved CT/CDT antitumor efficiency, but also realizes the activatable magnetic resonance imaging. This strategy combines the merits of each treatment, significantly enhancing the anticancer efficacy, and is anticipated to display promising potentials in the clinical translation plans.
研究领域:
纳米技术
·
癌症治疗
·
医学影像
·
药学科学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Energy Materials
·
2024
·
IF 5.4
Jiaxin Sun,Shengjie Li,Yitan Li,Xiaohua Zhang,Zhaohui Yang
Supercapacitors have been highly demanded as advanced energy storage devices. A major obstacle is that, compared to commercial battery systems, their energy density is relatively low. Here, we report a flexible asymmetric supercapacitor with high energy and power densities composed of nanostructured MnO2 nanospheres and Bi2O3 nanoshells decorated on a 3D interconnected carbon nanotube sponge (CNTS) in a neutral hydrogel-electrolyte. Our CNTS@MnO2 electrode and CNTS@Bi2O3 electrode demonstrate an excellent areal capacitance of 5.54 F·cm–3 and 4.76 F·cm–3, respectively. In 10,000 charge–discharge cycles, they maintain 93% and 80% of their initial capacitance, respectively. A flexible asymmetric supercapacitor composed of the CNTS@MnO2 anode and the CNTS@Bi2O3 cathode (embedded in a Na2SO4 hydrogel-electrolyte) delivers a high energy density up to 37 Wh·kg–1 at a cell voltage of 1.8 V, 7 times higher than that of traditional electrochemical double-layer capacitors (EDLCs). The long-term cyclic stability and robust mechanical stability profit from the flexible conductive skeleton of CNTS and meet the demands of wearable flexible electronics.
研究领域:
材料科学
·
电化学
·
纳米技术
·
能源存储
✓ 已验证
产品关联
DOI
高影响因子
Journal of Energy Storage
·
2024
·
IF 8.9
Quanwei Shi,Rui Mu,Wei Zhang,Chaoyue Zhang,Zhenhe Chang,Qi Cui,Baohui Wang,Di Gu
Herein, a novel rechargeable button zinc‑manganese ultra-high temperature battery is first reported for the efficient utilization and conversion of thermal and electrical energy. The battery system uses zinc anode , Zn-K-Cl molten salt electrolyte and MnO 2 /Mn 2 O 3 composite cathode to work at 300 °C. In this study, MnO 2 /Mn 2 O 3 composites were synthesized by molten salt method, and the crystal structure of the materials was adjusted by adding reduced graphene oxide (rGO). The results showed that the spherical nanoparticles and nanobelts of MnO 2 /Mn 2 O 3 composite cathode materials increased the active sites and ion transport channels of the electrochemical reaction , thus providing high coulombic efficiency. By modifying with rGO, the MnO 2 /Mn 2 O 3 (Mn-O-2) composite cathode material exhibits excellent electrochemical performance in the button zinc‑manganese ultra-high temperature battery. The as-prepared button ultra-high temperature battery exhibits a relatively high specific capacity (300 and 175 mAh g −1 at 1 mA cm −2 and 30 mA cm −2 ) and competent cyclability (> 95 % coulombic efficiency after 50 cycles). This work firstly and systematically reveals the role of the Mn cathode materials and shows that the application in button molten salt batteries.
研究领域:
材料科学
·
电化学
·
能源存储
·
电池技术
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）