Journal of Cleaner Production
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2023
·
IF 11.1
Di Zhang,Hao Tang,Bing Zhao,Lijie Liu,Hongwei Pang,Xiangxue Wang,Shujun Yu
Radioactive uranium removal plays a key role in energy development and environmental security. Sulfide nanoscale zero-valent iron (S-NZVI) has good antioxidant capacity, dispersibility and activity to eliminate U(VI) from wastewater, but the removal efficiency is limited. To improve the reduction and adsorption capacity of S-NZVI, through treating Fe 3+ solution by nitro-functionalized UiO-66 (UiO-66-NO 2 ) with high porosity, UiO-66-NO 2 enhanced S-NZVI adsorbent (S-NZVI/UiO-66) was prepared by liquid phase reduction method. The smaller size and larger specific surface area of S-NZVI/UiO-66 were confirmed by a variety of characterization techniques. Based on the excellent surface structure and reactivity, the U(VI) removal capacity of S-NZVI/UiO-66 (895 mg g −1 ) was much better than S-NZVI (434 mg g −1 ) and UiO-66-NO 2 (267 mg g −1 ). The impacts of pH, background ion strength, coexisting ions and different water environments on adsorption were studied via macroscopic batch experiments. The results showed that S-NZVI/UiO-66 could remove U(VI) adequately in a wide pH range. The removal rate was basically not affected by the concentration of NaNO 3 , demonstrating that the adsorption process of U(VI) on S-NZVI/UiO-66 tended to inner surface complexation. From XPS technique combined with the results of batch adsorption experiments, it was clear that the elimination mechanisms of U(VI) by S-NZVI/UiO-66 were mainly the physical adsorption, electrostatic attraction and complexation of UiO-66-NO 2 , and the reduction and complexation of S-NZVI. Therefore, it is believed that S-NZVI/UiO-66 has a great potential to be an excellent adsorbent in the field of the environment remediation. Moreover, S-NZVI/UiO-66 promises to be a novel nanomaterial for solving the problem of radionuclide contamination in practical environments.
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LANGMUIR
·
2023
·
IF 3.9
Jiaxin Cao,Siyihan Duan,Qian Zhao,Guangyuan Chen,Zeru Wang,Ruixi Liu,Lin Zhu,Tao Duan
The effective capture and deposition of radioactive iodine in the spent fuel reprocessing process is of great importance for nuclear safety and environmental protection. Three-dimensional (3D) fiber felt with structural diversity and tunability is applied as an efficient adsorbent with easy separation for iodine capture. Here, a bismuth-based silica aerogel fiber felt (Bi@SNF) was synthesized using a facile hydrothermal method. Abundant and homogeneous Bi nanoparticles greatly enhanced the adsorption and immobilization of iodine. Notably, Bi@SNF demonstrated a high capture capacity of 982.9 mg/g by forming stable BiI3 and Bi5O7I phases, which was about 14 times higher than that of the unloaded material. Fast uptake kinetics and excellent resistance to nitric acid and radiation were exhibited as a result of the 3D porous interconnected network and silica aerogel fiber substrate. Adjustable size and easy separation and recovery give the material potential as a radioactive iodine gas capture material.
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ACS Applied Materials & Interfaces
·
2023
·
IF 9.5
Xiuli Wang,Weiwei Fei,Zhifan Zhou,Mengjing Zhu,Yinghao Chang,Qilin Guo,Jia Guo,Changchun Wang
Magnetic-assisted DNA testing technology has attracted much attention in genetics, clinical diagnostics, environmental microbiology, and molecular biology. However, achieving satisfying DNA adsorption and desorption efficiency in real samples is still a big challenge. In this paper, a new kind of high-quality magnetic composite microsphere of MM@PGMA–PA-Ti4+ was designed and prepared for DNA extraction and detection based on the strong interaction of Ti4+ and phosphate groups. By taking the advantages of high magnetic susceptibility and high Ti4+ content, the MM@PGMA–PA-Ti4+ microspheres possessed remarkable extraction capacity for mimic biological samples (salmon sperm specimens) with saturated loadings up to 533.0 mg/g. When the DNA feeding amount was 100 μg and the MM@PGMA–PA-Ti4+ dosage was 1 mg, the adsorption and desorption efficiencies were 80 and 90%, respectively. The kinetic and equilibrium extraction data were found to fit well with the pseudo-second-order model and Freundlich isotherm model. Furthermore, the MM@PGMA–PA-Ti4+ microspheres were successfully employed for DNA extraction from mouse epithelial-like fibroblasts. The extraction ability (84 ± 4 μg/mg) and DNA purity were superior to the comparative commercial spin kits, as evaluated by electrophoresis assays and qPCR analysis. The experimental results suggest that the MM@PGMA–PA-Ti4+ microspheres possess great potential as an adsorbent for DNA purification from complex biological samples.
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BIOPROCESS AND BIOSYSTEMS ENGINEERING
·
2023
·Vol. 46
·期 8
·1175-1194
·
IF 3.8
Liu Siyuan,Liu Wei,Yin Hao,Yang Chunyan,Chen Jianxin
This work focused on the development of an inexpensive carbon source and the improvement of the fermentation-foam fractionation coupling system. The rhamnolipids production capacity of waste frying oil (WFO) was evaluated. The suitable bacterial cultivation of seed liquid and the addition amount of WFO was 16 h and 2% (v/v), respectively. A combined strategy of cell immobilization and oil emulsion avoid cell entrainment inside foam and improves the oil mass transfer rate. The immobilization conditions of bacterial cells into alginate-chitosan-alginate (ACA) microcapsules were optimized using the response surface method (RSM). Under the optimal conditions, rhamnolipids production using batch fermentation with immobilized strain reached 7.18 ± 0.23% g/L. WFO was emulsified into a fermentation medium using rhamnolipids as emulsifier (0.5 g/L). By monitoring dissolved oxygen, 30 mL/min was selected as a suitable air volumetric flow rate for fermentation-foam fractionation coupling operation. The total production and recovery percentage of rhamnolipids were 11.29 ± 0.36 g/L and 95.62 ± 0.38%, respectively. Graphical abstract
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Reaction Chemistry & Engineering
·
2023
·Vol. 8
·期 9
·2365-2374
·
IF 3.9
Hongbo Suo,Qi Qi,Xusheng Dai,Xinyue Geng,Qi Li,Jie Yang,Guoyun Liu,Renmin Liu,Lili Xu
Recently, ionic liquids (ILs) have shown potential in enzyme activation and stabilization. In this work, ILs consisting of imidazolium-based cations coupled with [Tf2N]− anions were grafted onto a magnetic Ca2+-based metal–organic framework (CaBDC), and the composites were then employed to immobilize lipase. The support of ILs/MCaBDC and immobilized porcine pancreatic lipase (PPL) of PPL-ILs/MCaBDC were thoroughly characterized and fully investigated. Compared with a wild enzyme, PPL-ILs/MCaBDC showed 2.16 times higher activity. After introducing the ILs into the supports, PPL-ILs/MCaBDC showed enhanced thermal, storage, and stability properties compared with PPL–MCaBDC. After 10 cycles, PPL-ILs/MCaBDC retained a higher (83.2%) fraction of its initial activity than PPL–MCaBDC (73.4%). Furthermore, the relationship between the support–enzyme interactions and the structural changes of immobilized lipase was also analyzed in detail. Application of circular dichroism (CD) spectroscopy analysis helped us understand the changes of the enzyme secondary structure. Therefore, this work further confirmed the effectiveness of ILs for enzyme immobilization.
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CHEMOSPHERE
·
2022
·
IF 8.943
Jackson Nkoh Nkoh,Ke-wei Li,Yang-xiao-xiao Shi,Jiu-yu Li,Ren-kou Xu
Phosphorus (P) availability in highly weathered soils is significantly influenced by the contents of iron (Fe)/aluminum (Al) oxides, clay minerals, and organic matter. With the increasing interest in biofertilizers (e.g. chitosan), it is important to understand how they affect P adsorption profiles on colloids of weathered soils rich in Fe/Al oxides. Thus, the effect of chitosan on the adsorption of P to colloids of hematite, gibbsite , Oxisol , and Ultisol was studied through electrokinetic measurements, spectroscopic analysis , and adsorption edge/isotherm profiles. The presence of chitosan significantly improved the surface positive charge and the decreasing trend of surface positive charge was slower for chitosan-treated colloids compared to the control with increasing pH. At pH 5.0, all the colloids were positively charged, with the oxides containing more positve charges than the soil colloids. At this pH value, the surface coverage capacity of P was 99.1, 61.6, 50.5, and 37.5 mmol kg⁻ 1 for Oxisol, Ultisol, hematite, and gibbsite, respectively. This suggests that clay minerals in soil colloids were vital in enhancing P adsorption. In the presence of chitosan, the surface coverage capacity of P was increased by 111%, 173%, 647%, and 488% for Oxisol, Ultisol, gibbsite, and hematite, respectively. Drawing inferences from spectroscopic analysis, citric acid desorption profile, and zeta potential analysis, we suggest that chitosan (CH) enhanced P adsorption by promoting the formation of (i) citric acid “undisplaceable” inner-sphere P complexes such as [Colloid-OP- O -CH] and [Colloid-OP- N -CH] , (ii) citric acid “displaceable” outer-sphere P complexes such as {[Colloid- O -CH]-OP} and {[Colloid- N -CH]-OP} , and (iii) water “leachable or soluble” P complexes such as {[Colloid-CH] + PO 4 ³⁻} and {[Colloid-OP]⁻CH + } . Thus, applying chitosan as a biofertilizer (source of N) along with P in highly weathered soils could improve P availability while reducing P leaching .
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Journal of Oleo Science
·
2022
·
IF 1.628
Lihong He,Jiawei Zheng,Siting Feng,Li Xu,Nanjing Zhong
In this study, lipase A from Candida antarctica (CALA) was immobilized onto the macroporous resin NKA-9. Immobilization conditions (pH, time and CALA concentration) were studied, enzymatic activity and immobilization efficiency (IE) up to 968.89 U/g and 53.19% were respectively obtained under optimal conditions (immobilization pH 5.0, time 5 h and CALA concentration at 30 mg/mL). Then, the NKA-9 supported CALA (CALA@NKA-9) samples were used to catalyze glycerolysis in solvent-free system. With 0.25 g of the present CALA@NKA-9 (soybean oil 3.52 g and glycerol 0.184 g) and after 12 h reaction at 50 °C, diacylglycerols (DAG) content up to 64.37% and triacylglycerols (TAG) conversion at 83.33% were obtained. The relationship between temperature and TAG conversion was LnV 0 = 13.9310-6.4212/T for CALA@NKA-9. Meanwhile, the activation energy (Ea) of CALA@NKA-9 was calculated to be 53.39 kJ/mol. In addition, reusability in the glycerolysis reaction was also evaluated, and 57.82% of the initial glycerolysis activity was retained after 9 consecutive applications. Furthermore, the CALA@NKA-9 was also used to catalyze the esterification (esterification of fatty acids with glycerol), however, the present CALA@NKA-9 cannot initiate the esterification. Therefore, the present CALA@NKA-9 is shown to be potential for DAG production through glycerolysis reaction.
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Journal of Physical Chemistry C
·
2022
·
IF 4.177
Wenbin Yu,Shaobo Ma,Mengxue He,Ruinan Li,Hui Yang,Yong Li,Geping Yin,Pengjian Zuo
Inhibiting the shuttle effect and accelerating the reaction kinetics of soluble lithium polysulfides (LiPS) are crucial for constructing high-performance lithium–sulfur (Li–S) batteries. Herein, a novel iodine-doped MXene (I-MXene)-modified separator is proposed to optimize the electrochemical performance of Li–S batteries. The I-MXene composite exhibits a uniform two-dimensional layer structure and high electronic conductivity, which are beneficial for building high conductivity and a thin/uniform coating layer. Additionally, the I-MXene can effectively immobilize LiPS through Ti–S bonds and accelerate the reaction kinetics of LiPS conversion. When combined with the KB/S cathode, the cell using the I-MXene-modified separator displays a high initial discharge capacity of 1308 and 761 mAh g–1 after 100 cycles at 0.2 C, as well as high C-rate capability (655 mAh g–1/2 C). With a sulfur area loading above 5 mg cm–2, the cell matching the I-MXene/PP separator still delivers an attractive reversible capacity of 578 mAh g–1 after 200 cycles at 0.2 C. The novel catalytic two-dimensional materials and separator modification method proposed here are expected to facilitate the practical application of Li–S batteries.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2022
·
IF 8.025
Ling Zheng,Zilong Guo,Yinxiao Xu,Benwei Zhu,Zhong Yao
Pectate lyase (ErPL2) from Echinicola rosea JL3085 showed maximal activity at 45 °C and pH 9.0 with 0.6 mM CaCl 2 . The K m and V max values of ErPL2 for polygalacturonic sodium were 2.098 mmol/L and 0.955 mmol/s, respectively. ErPL2 endolytically degraded pectic substances into oligosaccharides with degree of polymerization (DP) 1–5. To improve the thermostability and pH operation range, recombinant ErPL2 was immobilized onto mesoporous titanium oxide particles (MTOPs). MTOPs have abundant hydroxyl groups on the surface, which is a non-toxicity and good biocompatibility carrier. The residual enzyme activity of immobilized ErPL2 at 40 °C increased remarkably from 11% to 91% compared with free enzyme. The operable pH range was extended from 8–9 to 9–11. Surprisingly, the catalytic efficiency of immobilized ErPL2 was about 19 times higher than free enzyme. To our knowledge this is the first attempt of pectate lyase immobilized on MTOPs and it provides a new option for improving the catalytic performance.
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Separation Science Plus
·
2022
·Vol. 5
·期 6
·237-246
·
IF 0
Min Lu,Hao Zhang,Shi-Jun Yin,Hui Jiang,Xu Wang,Feng-Qing Yang
In this study, the poly (sodium 4-styrenesulfonate)-mediated calcium carbonate magnetic microspheres were prepared by a simple spontaneous biomimetic mineralization method to immobilize kallikrein. The immobilized kallikrein can be easily separated from the reaction mixture by an external magnet. The structural properties of the materials were studied by several characterization techniques. Subsequently, the immobilized kallikrein enzyme activity and stability were studied. Moreover, the inhibitory potency of ten natural compounds on kallikrein was evaluated by combining with capillary electrophoresis analysis. The immobilized kallikrein exhibited optimum pH and temperature at 7.5 and 75°C, respectively. After seven successive cycles, the immobilized kallikrein retained 82% of its initial activity. Furthermore, the Michaelis–Menten constant ( K m ) and the half-maximal inhibitory concentration (IC 50 ) of serine protease inhibitor on the immobilized kallikrein were determined to be 3.30 mM and 67.04 μM, respectively. In addition, berberine, evodine, coptisine, and jatrorrhizine among the ten natural compounds showed good inhibitory activity on the immobilized kallikrein, and their % of inhibition were (43.1 ± 1.7), (34.4 ± 1.9), (38.0 ± 1.7), and (28.5 ± 1.0)%, respectively. These results indicate that the biomimetic mineralization synthetic magnetic microsphere is an efficient method for kallikrein immobilization.
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CHEMBIOCHEM
·
2021
·Vol. 23
·期 4
·e202100497
·
IF 3.164
Kangjing Wang,Liting Zhao,Ting Li,Qian Wang,Zhongyang Ding,Weifu Dong
Graphical Phosphomannose isomerase (PMI) with His-tag was successfully immobilized on Ni 2+ charged pyridine-derived particles. The immobilized enzymes effectively maintained their activity. Excellent reusability and stability was observed after immobilization. Self-stable precipitation polymerization was used to prepare an enzyme-immobilized microsphere composite. Phosphomannose isomerase (PMI) with His-tag was successfully immobilized on Ni 2+ charged pyridine-derived particles. The maximum amount of PMI immobilized on such particles was ∼184 mg/g. Compared with free enzyme, the activity of the immobilized enzymes was significantly improved. In addition, the immobilized enzymes showed a much better thermostability than free enzymes. At the same time, the immobilized enzymes can be reused for multiple reaction cycles. We observed that the enzyme activity did not decrease significantly after six cycles. We conclude that the pyridine-derived particles can be used to selectively immobilize His-tagged enzymes, which can couple the enzyme purification and catalysis steps and improve the efficiency of enzyme-catalyzed industrial processes.
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DALTON TRANSACTIONS
·
2023
·Vol. 52
·期 26
·9084-9089
·
IF 4
Xiaodong Feng,Lihui Liu,Yeming Wang,Chaoqun Zhang,Gang Liu,Yuyang Tian,Guangshan Zhu
A hierarchical metal–organic framework, H-mMOF-1 (representing hierarchical medi-MOF-1), was successfully synthesized by the coassembly of MOF starting reagents and a triblock copolymer surfactant F127. The obtained H-mMOF-1 retained its microporous structure but also exhibited mesopores with a size range from 3 to 10 nm. The mesopores were able to accommodate protein Cyt c with a loading capacity of 160 mg g−1. The surfactant-assisted synthesis of hierarchical MOFs provides promising applications for enzyme immobilization.
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