Food Chemistry-X
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2025
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IF 8.2
Lisha Lan,Li Chen,Xiaolin Zhong,Weiguo Cao,Ying Zhou,Jing Wan,Yuanyuan Liu,Qinjin Zhu
This study examined the effects of electrical stimulation on dry-cured tenderloin at different processing stages (0, 4, and 11 days), with a primary focus on lipid oxidation and the composition of volatile compounds. The study's results revealed that electrical stimulation enhanced lipase activity and accelerated lipid oxidation. Compared to the control group, the electrically stimulated samples exhibited higher total amino acid and fatty acid contents. Furthermore, the electrically stimulated group demonstrated superior taste (as assessed by an electronic tongue) and aroma profiles (as assessed by an electronic nose). Among the 49 compounds identified by GC–MS, the electrically stimulated dry-cured tenderloin displayed a greater diversity of volatile compounds. Notably, certain compounds, including ethyl lactate, ethyl myristate, 2,3-octanedione, and cyclohexanone, were exclusively detected in the electrically stimulated samples. These findings offer novel insights into the interplay between lipid oxidation and flavor development during meat processing via electrical stimulation.
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高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Junxin Zhao,Maomao Ma,Xianghui Yan,Guohua Zhang,Jiaheng Xia,Zheling Zeng,Ping Yu,Qiang Deng,Deming Gong
In this study, the polydopamine functionalized magnetic mesoporous biochar (MPCB-DA) was prepared for immobilization of Bacillus licheniformis lipase via covalent immobilization. Under optimized immobilization conditions, the maximum immobilization yield, efficiency and immobilized lipase amount were found to be 45%, 54% and 36.9 mg/g, respectively. The immobilized lipase, MPCB-DA-Lipase showed good thermal stability and alkali resistance. The MPCB-DA-Lipase retained 56% initial activity after 10 reuse cycles, with more than 85% relative activity after 70 days’ storage at 4 or 25 °C. The MPCB-DA-Lipase was efficiently applied in the interesterification of Cinnamomum camphora seed kernel oil and perilla seed oil, with maximum interesterification efficiency of 46%. The produced structured lipids belong to the S 2 U and U 2 S triacylglycerols, a novel medium-and long-chain triacylglycerol. These results demonstrated that the MPCB-DA-Lipase may be used as an efficient biocatalyst in lipid processing applications of food industries.
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高影响因子
Frontiers in Bioengineering and Biotechnology
·
2021
·
IF 5.89
Wang Zhaoyu,Li Yang,Li Mingyi,Zhang Xiaohui,Ji Qingxia,Zhao Xiaojuan,Bi Yanhong,Luo Si
The conversion of flavonoid glycosides and their analogs to their lipophilic ester derivatives was developed by nanobiocatalysts from immobilizing Thermomyces lanuginosus lipase (TLL) on polydopamine-functionalized magnetic Fe3O4 nanoparticles (Fe3O4-PDA-TLL). The behavior investigation revealed that Fe3O4-PDA-TLL exhibits a preference for long chain length fatty acids (i.e., C10 to C14) with higher reaction rates of 12.6–13.9 mM/h. Regarding the substrate specificity, Fe3O4-PDA-TLL showed good substrate spectrum and favorably functionalized the primary OH groups, suggesting that the steric hindrances impeded the secondary or phenolic hydroxyl groups of substrates into the bonding site of the active region of TLL to afford the product.
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PeerJ
·
2017
·
IF 2.177
Qiu Jin,Huahua Yu,Xueqin Wang,Kecheng Li,Pengcheng Li
Background Obesity has become a worldwide burden to public health in recent decades. Given that obesity is caused by an imbalance between caloric intake and expenditure, and that dietary fat is the most important energy source of all macronutrients (by providing the most calories), a valuable strategy for obesity treatment and prevention is to block fat absorption via the gastrointestinal pathway. In this study, the fat- and cholesterol-binding capacities and the inhibition of pancreatic lipase by water-soluble chitosan (WSC) with different weight-average molecular weight (Mw) were tested and compared in vitro , in order to determine the anti-obesity effects of WSC and the influence of its Mw. Methods In this study, WSC with different Mw (∼1,000, ∼3,000, ∼5,000, ∼7,000 and ∼9,000 Da) were prepared by oxidative degradation assisted with microwave irradiation. A biopharmaceutical model of the digestive tract was used to determine the fat- and cholesterol-binding capacity of WSC samples. The pancreatic lipase assays were based on p-nitrophenyl derivatives. Results The results showed that all of the WSC samples exhibit great fat- and cholesterol-binding capacities. Within the testing range, 1 g of WSC sample could absorb 2–8 g of peanut oil or 50–65 mg of cholesterol, which are both significantly higher than the ability of cellulose to do the same. Meanwhile, all the WSC samples were proven to be able to inhibit pancreatic lipase activity to some extent. Discussion Based on the results, we suggest that there is a significant correlation between the binding capacity of WSC and its Mw, as WSC2 (∼3,000 Da) shows the highest fat- and cholesterol-binding capacities (7.08 g g −1 and 63.48 mg g −1 , respectively), and the binding ability of WSC declines as its Mw increases or decreases from 3,000 Da. We also suggest WSC as an excellent resource in the development of functional foods against obesity for its adsorption, electrostatic binding and entrapment of cholesterol, fat, sterols and triglycerides in the diet.
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高影响因子
BIORESOURCE TECHNOLOGY
·
2022
·
IF 11.889
Miao Tian,Lingmei Yang,Pengmei Lv,Zhiyuan Wang,Junying Fu,Changlin Miao,Zhibing Li,Lianhua Li,Tao Liu,Wenyi Du,Wen Luo
Exploiting highly active and methanol-resistant lipase is of great significance for biodiesel production. A semi-rational directed evolution method combined with N-glycosylation is reported, and all mutants exhibiting higher catalytic activity and methanol tolerance than the wild type (WT). Mutant N267 retained 64% activity after incubation in 50% methanol for 8 h, which was 48% greater than that of WT. The catalytic activity of mutants N267 and N167 was 30- and 71- fold higher than that of WT. Molecular dynamics simulations of N267 showed that the formation of new strong hydrogen bonds between glycan and the protein stabilized the structure of lipase and improved its methanol tolerance. N267 achieved biodiesel yields of 99.33% (colza oil) and 81.70% (waste soybean oil) for 24 h, which was much higher than WT (51.6% for rapeseed oil and 44.73% for wasted soybean oil). The engineered ProRML mutant has high potential for commercial biodiesel production.
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MICROPOROUS AND MESOPOROUS MATERIALS
·
2019
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IF 4.182
Meng Chen,Haoming Wu,Zhentao Li,Keke Wu,Yanpeng Jiao,Changren Zhou
In this study, the effect of the hydrophobicity of reduced graphene oxide (rGO) on the activity of candida rugose lipase (CRL) was investigated firstly. It was observed that the increasing hydrophobicity of rGO surface could increase CRL activity, while excessive hydrophobicity acted to depress its activity. Then, reduced graphene oxide beads (rGOB) had been fabricated through a facile self-assembly method. The morphology, specific surface area, and mechanical properties of the rGOB were investigated by scanning electron microscopy (SEM), Brunauer-Emmett-Teller (BET), and static mechanics properties test, respectively. The results revealed that the rGOB showed a three-dimensional micro-nano porous structure and surface hydrophobicity, which played an important role in improving their adsorption properties and catalytic activity of CRL. CRL was directly immobilized onto the beads by physical adsorption with the maximum adsorption capacity of 117.67 mg/g under the optimized condition. The immobilized lipase (rGOB@CRL) exhibited good temperature, thermal, and reuse stability in comparison to free CRL. In the glycerolysis reaction of olive oil, the conversion rate of triglycerides (TG) in free enzyme reaction system was 67.83%, while the conversion rate of TG in rGOB 4 @CRL reaction system could reach 74.87%, which was higher than that of the former. Moreover, rGOB@CRL was further used to adsorb emulsified oil due to the excellent oil-absorbing ability of rGOB, and then producing monoglyceride (MG) and diglyceride (DG) in situ by catalyzing the glycerolysis reaction. When the oil concentration was 4 mg/mL, the conversion rate of triglyceride (TG) could reach 89.70%.
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RSC Advances
·
2015
·Vol. 5
·期 86
·70073-70079
·
IF 3.84
Mingming Zheng,Jiuxia Zhu,Fenghong Huang,Xia Xiang,Jie Shi,Qianchun Deng,Fangli Ma,Yuqi Feng
Rice bran oil (RBO) is a great and important vegetable oil resource, but usually contain high content of free fatty acids (FFAs). Phytosterol esters are functional lipids possessing hypocholesterolemic activity. In this study, Candida rugosa lipase (CRL) was immobilized on the octyl-functionalized ordered mesoporous silica (C8-OMMs) to obtain immobilized CRL arrays (CRL@C8-OMMs). The resulting CRL@C8-OMMs arrays showed much better thermal stability and reusability in comparison to free CRL. The deacidification and simultaneous preparation of functional oil rich in phytosterol esters (PEs) was successfully achieved by enzymatic esterification of high-acid RBO with phytosterol. The contents of FFAs and PEs were below 2.3% and above 84.7%, respectively. Repeated reaction tests indicated that CRL@C8-OMSs arrays could be used 11 times with 80.7% of its original catalytic activity still retained. The strategy could not only take advantage of the high-acid oils resources, but also product PEs-enriched functional oils.
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FOOD CHEMISTRY
·
2024
·
IF 8.5
Jinhang Chen,Tieliang Liu,Yi Zhang,Lu Zheng,Kheng-Lim Goh,Vladimir Zivkovic,Mingming Zheng
Flavor esters are used in food and cosmetic industries, but sustainable production remains a big challenging. This study proposed the ultrasound-assisted biosynthesis of cinnamyl acetate using self-made immobilized lipase CSL@OMS-C 8 , with computational fluid dynamics (CFD) and molecular simulations revealing the hydrodynamic properties and lipase-catalytic mechanisms. The results demonstrate that ultrasonication-intensified enzymatic reaction facilitated 96.6 % conversion of cinnamic alcohol, due to the ultrasound-assisted catalytic efficiency of 13.7 mmol/g·min. Molecular docking analysis identifies the lowest binding energy of −3.7 kcal·mol −1 between lipase and vinyl acetate, contributing to the highest conversion rates compared to acetic acid, ethyl acetate. CFD simulation indicates that ultrasonic energy waves promote substrate diffusion and mixing with lower shear stress. The catalytic stability of CSL@OMS-C 8 was confirmed by 60.1 % of relative activity after 10-time reuse. This paper theoretically and experimentally studied the ultrasonic-assisted enzymatic synthesis of cinnamyl acetate, showcasing huge potential for sustainable production of flavor esters.
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FOOD RESEARCH INTERNATIONAL
·
2026
·
IF 8.8
Weiqi Liu,Chong Liu,Shuang Cheng,Zhihao Zhao,Yuanyuan Deng,Guang Liu,Zexin Zhao,Pengfei Zhou,Mingwei Zhang
A novel oil-in-natural deep eutectic solvent (O/NADES) PIB platform was developed for production of DAG. • Better performance for production of DAG was obtained in O/NADES and O/glycerol Pickering emulsion droplets. • The insights of thermostability of CALB were proposed with molecular dynamics simulations. Glycerolysis is a crucial approach for the preparation of diacylglycerols (DAG). The challenges such as enzyme stability and interfacial mass transfer have been insufficiently addressed. To validate the Pickering interfacial biocatalysis (PIB) for the desired target, a boosting system containing Pickering emulsion droplets based on natural deep eutectic solvents (NADES) was developed as a reaction medium for enhancing the catalysis efficiency of CALB-catalyzed ( Candida antarctica lipase B, CALB) glycerolysis. The effects of types of oils and NADES on the properties of Pickering emulsions and catalytic efficiency were evaluated. Comparative analyses within the system revealed a 3.20-fold enhancement in catalytic efficiency for the NADES-based Pickering emulsion compared to its non-emulsion control, and an independent 9.13-fold enhancement for the glycerol-based Pickering emulsion relative to corresponding non-emulsion control. Additionally, molecular dynamics simulations offered insights into the temperature-dependent conformational behavior of CALB across various polar media. This study highlights the potential applicability of NADES Pickering emulsions as an efficient and sustainable platform for biocatalysis of lipids. Download: Download high-res image (118KB) Download: Download full-size image
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PHYTOCHEMICAL ANALYSIS
·
2023
·
IF 3.3
Sihua Quan,Mengyi Wen,Ping Xu,Chu Chu,Hui Zhang,Kai Yang,Shengqiang Tong
Introduction Pancreatic lipase is one of the most important key targets in the treatment of obesity. Inhibition of pancreatic lipase can effectively reduce lipid absorption and treat obesity and other related metabolic disorders. Objectives The goal of this study is the efficient screening of pancreatic lipase inhibitors in the root and rhizome of Rheum palmatum using affinity ultrafiltration–high-performance liquid chromatography (AUF-HPLC) combined with high-resolution inhibition profiling (HRIP). Methods Potential pancreatic lipase ligands and pancreatic lipase inhibitors in ethyl acetate fraction of R. palmatum were screened using AUF-HPLC and HRIP, respectively. All screened compounds were identified by HPLC– quadrupole time-of-flight (Q-TOF)/MS. Eight compounds were screened out by both AUF-HPLC and HRIP, and six compounds were screened out by either AUF-HPLC or HRIP alone. The pancreatic lipase inhibitory activities of all screened compounds were verified by enzyme inhibition assay and molecular docking. Results Five new potent pancreatic lipase inhibitors were discovered, namely procyanidin B5 3,3′-di-O-gallate (IC 50 = 0.06 ± 0.01 μM), 1,6-di-O-galloyl-2-O-cinnamoyl-β-D-glucoside (IC 50 = 12.83 ± 0.67 μM), 1-O-(1,3,5-trihydroxy)phenyl-2-O-galloyl-6-O-cinnamoyl-β-D-glucoside (IC 50 = 17.84 ± 1.33 μM), 1,2-di-O-galloyl-6-O-cinnamoyl-β-D-glucoside (IC 50 = 18.39 ± 1.52 μM), and 4-(4′-hydroxyphenyl)-2-butanone-4’-O-β-D-(2”-O-galloyl-6”-O-cinnamoyl)-glucoside (IC 50 = 2.91 ± 0.40 μM). It was found that procyanidin B5 3,3′-di-O-gallate showed higher pancreatic lipase inhibitory activity than the positive control orlistat (IC 50 = 0.12 ± 0.02 μM). Conclusion The combination of affinity ultrafiltration–high-performance liquid chromatography (AUF-HPLC) and high-resolution inhibition profiling (HRIP) could reduce the risk of false-negative screening and missed screening and could achieve more efficient screening of bioactive compounds in complex natural products.
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RENEWABLE ENERGY
·
2022
·
IF 8.634
Zhijin Zhang,Yingjie Du,Geling Kuang,Xuejian Shen,Xiaotong Jia,Ziyuan Wang,Yuxiao Feng,Shiru Jia,Fufeng Liu,Muhammad Bilal,Jiandong Cui
The production of biodiesel catalyzed by lipase has the advantages of simple process, low energy consumption, and low wastewater treatment requirements. However, low activity, poor stability, and difficulty in recycling still limit the application of lipase in production of biodiesel. Herein, a novel lipase hybrid biocatalyst with superior catalytic activity and stability (lipase from Aspergillus oryzae CJLU-3, AOCL@CaP in DES) was developed by dual activation of Ca 2+ and interfacial effect of deep-eutectic solvents (DES)/water two-phase system. AOCL@CaP in DES exhibited significantly enhanced enzymatic activity with a K cat /K m value of 692 s −1 mM −1 and excellent thermostability, tolerance to chemical denaturants, substrate selectivity, and reusability compared with free lipase. Recovery activity of the prepared hybrid biocatalyst in pure water system (AOCL@CaP) and AOCL@CaP in DES was 195% and 250% using p-NPL as substrate, respectively. AOCL@CaP in DES exhibited about 253% of the original activity after 100 days of storage, while free AOCL, AOCL@CaP almost completely lost activity. The FAME content in product biodiesel catalyzed from soybean oil by AOCL@CaP was only 60%, However, the FAME content catalyzed by AOCL@CaP in DES reached 87%, and could be maintained at 50% even after 10 cycles.
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LWT-FOOD SCIENCE AND TECHNOLOGY
·
2022
·
IF 6.056
Chunyun Ma,Yan Zhang,Chuankai Yang,Yuhang Zhang,Miaorong Zhang,Jianguo Tang
Cetyl trimethyl ammonium bromide (CTAB)-activated lipase-mineral hybrid microflowers (CTAB-Lip-MHFs) were fabricated via biomineralization using Cu 3 (PO 4 ) 2 ⋅3H 2 O as the mineral for olive oil hydrolysis. FT-IR spectra indicated that immobilized lipase molecules in CTAB-Lip-MHFs has good structural integrity, and XPS spectra revealed that immobilized lipase molecules were present at the surface of CTAB-Lip-MHFs. Kinetic studies suggested that CTAB-Lip-MHFs had good substrate affinity ascribed to interfacial effect caused by CTAB, meanwhile, the open conformation of lipase activated by CTAB contributed to increased reaction rate with substrate. The generated fatty acids via hydrolysis of olive oil by CTAB-Lip-MHFs were 146% of that by free lipase, moreover, CTAB-Lip-MHFs could retain 76.5% and 93.2% of initial activities after storage at 50 °C for 10 h and 4 °C for 30 days, respectively, indicating the good stability of CTAB-Lip-MHFs. In addition, after 5 recycles, CTAB-Lip-MHFs preserved 72.7% of initial activity, demonstrating the favorable reusability of CTAB-Lip-MHFs for fatty acid production.
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危险品化学品经营许可证（带存储）