Brazilian Journal of Pharmaceutical Sciences
·
2023
·
IF 1.3
Zhao Li-Li,Ma Qingkun,Xu Chun-Yan,Zheng Guanghao,Han Yu,Qian Qing,Jin Wenlong,Min Jun Zhe
Serum uric acid (UA) is a traditional biomarker in the clinical diagnosis of gout and hyperuricemia. However, serum treatment and storage are cumbersome, and wounds are susceptible to infection. Therefore, in this study, a simple and noninvasive method was developed to detect the UA in human saliva to monitor the gout. An Inertsil ODS-3 column was used for the analysis under the condition of isocratic elution with the mixed solution phosphate buffer (74 mM, pH=2.2): Methanol=98:2 (v:v) and the UV detection at 284 nm. Using salivary UA data from healthy volunteers (HVs) (n=68) and gout patients (GPs) (n=14), we examined the salivary UA difference in their content. The intra-and inter-day accuracy and precision (RSD %) were less than 2.56%, the limit of detection (LOD) of UA was 5.0 ng/mL, the mean recoveries of the corresponding compounds were 102.48%. Saliva levels of UA in HVs and GPs were 35.26±14.06 µg/mL and 91.96±23.90 µg/mL, respectively. The concentrations of salivary UA in GPs were significantly higher than those in HVs ( p < 0.001). This method was also expected to monitor the hyperuricemia and other metabolic disorders in the future.
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MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Sizhe Zhang,Qi Pu,Xinyi Deng,Lu Zhang,Nengsheng Ye,Yuhong Xiang
Choline (ChO) is a biomarker of many diseases. Its accurate and sensitive detection is of great significance for the diseases diagnosis. Gold nanoclusters with bovine serum albumin as template (BSA-AuNCs) were synthesized by microwave-assisted method. A ratiometric fluorescence sensor was constructed based on BSA-AuNCs and 2,3-diaminophenazine, which was the oxidation product of o-phenylenediamine. Combined with cascade enzymatic reaction, it was used for the detection of choline. In the detection system, choline oxidase improved the selectivity of detection, and the peroxidase-like activity of molybdenum disulfide nanosheets shortened the detection time. The linear ranges of choline detection by ratiometric fluorescence sensor were 0.5–20 μM and 20–100 μM. The detection limit was 0.27 μM. The ratiometric fluorescence sensor was easy to operate and had good selectivity. Compared with single signal sensor, it can effectively reduce the error interference such as environmental change, and the detection result was more accurate.
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ANALYTICAL CHEMISTRY
·
2023
·
IF 7.4
Duo Chen,Na Chen,Fangning Liu,Yiming Wang,Huageng Liang,Yanbing Yang,Quan Yuan
Portable point-of-care testing (POCT) is currently drawing enormous attention owing to its great potential for disease diagnosis and personal health management. Electrochemical biosensors, with the intrinsic advantages of cost-effectiveness, fast response, ease of miniaturization, and integration, are considered as one of the most promising candidates for POCT application. However, the clinical application of electrochemical biosensors-based POCT is hindered by the decreased detection sensitivity due to the low abundance of disease-relevant biomolecules in extremely complex biological samples. Herein, we construct a flexible electrochemical biosensor based on single-stranded DNA functionalized single-walled carbon nanotubes (ssDNA-SWNTs) for high sensitivity and stability detection of miRNA-21 in human urine to achieve bladder cancer (BCa) diagnosis and classification. The ssDNA-SWNT electrodes with a 2D interconnected network structure exhibit a high electrical conductivity, thus enabling the ultrasensitive detection of miRNA-21 with a detection limit of 3.0 fM. Additionally, the intrinsic flexibility of ssDNA-SWNT electrodes endows the biosensors with the capability to achieve high stability detection of miRNA-21 even under large bending deformations. In a cohort of 40 BCa patients at stages I–III and 44 negative control samples, the constructed ssDNA-SWNT biosensors could detect BCa with a 92.5% sensitivity, an 88.6% specificity, and classify the cancer stages with an overall accuracy of 81.0%. Additionally, the flexible ssDNA-SWNT biosensors could also be utilized for treatment efficiency assessment and cancer recurrence monitoring. Owing to their excellent sensitivity and stability, the designed flexible ssDNA-SWNT biosensors in this work propose a strategy to realize point-of-care detection of complex clinical samples to achieve personalized healthcare.
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高影响因子
Journal of Materials Chemistry B
·
2023
·Vol. 11
·期 7
·1523-1532
·
IF 7
Zhong-Xia Wang,Lei Hu,Xiao-Qiong Li,Yi-Lei Jia,Ting Wang,Wei Wang
Exploring the ultrahigh quantum efficiency of a carbon-based probe via a green and simple technique, and utilisation of its sensing ability for highly bioactive molecule detection is still highly challenging. Herein, we prepared a novel boron-enriched rice-like homologous carbon nanoclusters (BRCNs) with an ultrahigh quantum efficiency of ∼51.5% by introduction of a conjugated structure attached to the CN bond and an electron-withdrawing boron active centre. Unexpectedly, the BRCNs obtained showed a stable dispersion of rice-like carbon nanograins, composed of small carbon dot assembled nanoclusters with an average diameter size of ∼30 nm, and containing boron units of ∼24.68 at%. What's exciting is that the BRCNs obtained exhibited an “on–off–on” three-state emission with the addition of an hydroxyl radical (OH˙) and its antioxidants. Thus, two distinctive fluorescent responses for OH˙ and antioxidants based on the BRCN probe had been developed, and the mechanism has been determined using TEM, XPS, FT-IR, FL, UV-vis spectrophotometry, UPS and fluorescent lifetimes. The OH˙, generated from the Fenton's reagent, preferentially attack the electron-deficient vacancy p orbit of the boron atom in the surface of the BRCNs, which results in the boron atom being easily substituted/attacked by OH˙, and leading to spontaneous aggregation induced quenching (AIQ) due to the existence of a strong intermolecular hydrogen bond between denatured BRCNs. Furthermore, the proposed method was also successfully applied to monitor endogenous OH˙ generation in HeLa cells by confocal imaging, which could be used for elucidating OH˙-induced oxidative damage to biological tissues and proteins.
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CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
·
2022
·
IF 1.193
Jin-Ling PU,Pei-Hong TONG,Yong-Juan MENG,Jian-Ping LI
A bilayer-structured ZIF-8 composite with dual functions of sensing and recognition was synthesized, with ZIF-8 serving as an electrochemiluminescence (ECL) probe carrier and molecularly imprinted membrane to construct a molecularly imprinted electrochemiluminescence sensor. The inner layer was a ZIF-8 layer loaded with a luminescent probe, which was obtained by the reaction of zinc ions with 2-methylimidazole on the magnetic Au@CoFe 2 O 4 surface in the presence of Ru(bpy) 3 2+ , the composite magnetic particle is marked as ZIF(Ru)/Au@CoFe 2 O 4 . The outer layer was a layer with recognition function using ZIF-8 as an imprinted membrane, which was obtained by a layer-by-layer self-assembly method on the surface of ZIF(Ru)/Au@CoFe 2 O 4 with dopamine (DA) as a template molecule, which was marked as ZIF(MIP)/ZIF(Ru)/Au@CoFe 2 O 4 . After eluting the template molecules, the composite particle was immobilized on the surface of a magnetically controlled glassy carbon electrode (MGCE) to obtain a molecular imprinting (MIP) modified electrode. When dopamine molecules were re-adsorbed by the MIP, a charge/energy transfer between dopamine and Ru(bpy) 3 2+ occurred. It subsequently led to the quenching of electrochemiluminescence, which was used as the basis for the detection of dopamine by a molecularly imprinted electrochemiluminescence sensor. When the dopamine concentration was in the range of 1.0 × 10 −14 –5.0 × 10 −10 mol/L, the ECL intensity change of the sensor and the dopamine concentration had a good linear relationship, and the detection limit was 4.8 × 10 −15 mol/L. The molecularly imprinted electrochemiluminescence sensor was successfully applied to the detection of dopamine in human serum samples.
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ELECTROCHIMICA ACTA
·
2022
·
IF 7.336
Bo Shao,Fang Chen,Jianyuan Wang,Wei Zhai
Sonochemical synthesis is recognized as a promising high-efficiency method for fabricating transition metal oxides (TMOs) based flexible self-supported electrodes as nonenzymatic glucose sensor. However, it is still a challenge to obtain TMOs self-supported electrodes with well-controlled TMOs nanostructure and desirable interfacial properties via a sonochemical approach because of the uncontrollability of transient cavitation. In this study, CuO@polydopamine (PDA) nanoparticles (NPs) are in situ grown onto carbon cloth (CC) to fabricate CuO@PDA/CC self-supported electrode via a sonochemical process. The sonochemically synthesized PDA not only stabilized the transient cavitation intensity, but also regulated the growth of CuO due to the strong chelating ability between Cu 2+ and catechol groups from PDA. It is determined that the mean size of CuO@PDA NPs on the CuO@PDA/CC electrode is less than 100 nm with a narrow size distribution. Attributed to high electrochemical active surface area (4.61 mF cm −2 ) and low interface resistance (2.35 Ω), CuO@PDA/CC electrode displays excellent electrochemical performance as a glucose sensor with high sensitivity of 1.843 μA cm −2 mM −1 in a wide linear range up to 4.985 mM and excellent stability. This work provides a facile approach to fabricating the flexible self-supported electrodes with well-controlled nanostructure and desirable interfacial properties for high-performance electrochemical sensors via regulating the transient cavitation intensity in the sonochemical process.
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JOURNAL OF ALLOYS AND COMPOUNDS
·
2022
·
IF 6.371
Aijuan Xie,Haoye Wang,Jiaqi Lin,Jie Pan,Menglu Li,Jiaojie Wang,Shixin Jiang,Shiping Luo
In the current study, bimetallic-organic gel (ZrFe-MOGs) were rapidly prepared without using any toxic or harmful organic solvent , and new bimetallic oxide ZrFeO x were prepared by roasting ZrFe-MOGs directly. Then ZrFeO x nanoparticles were combined with 3D RGO/MWCNTs by a solvothermal method to synthesize 3D RGO/MWCNTs@ZrFeO x hybrid. Due to its three-dimensional structure, large electroactive surface area and more active sites, and excellent conductivity, the electrocatalytic activity of the modified electrode for the oxidation of dopamine (DA) and paracetamol (PAT) was effectively improved, and the overlap of anode peak due to their closer electrochemical oxidation potentials was solved. Under optimal conditions, the linear responses to DA and PAT were in the range of 1 − 12 μM, 12 − 180 μM and 1 − 14 μM, 14 − 190 μM, respectively. The detection limits (S/N = 3) for DA and PAT were 0.230 and 0.212 μM, respectively. The optimized 3D RGO/MWCNTs@ZrFeO x has good reproducibility, repeatability, stability, selectivity, and can be used to detect DA and PAT simultaneously. In addition, the proposed RGO/MWCNTs@ZrFeO x sensor exhibited a good recovery factor in the analysis of real drug and serum samples with a relative standard deviation of less than 5 %. Importantly, the electroactive area of 3D RGO/MWCNTs@ZrFeO x , and the 2 H + /2e - mechanism of DA and PAT oxidation on the surface of 3D RGO/MWCNTs@ZrFeO x were discussed, which provided a theoretical basis for further understanding of this redox process .
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MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Tianlu Zhao,Yangqing Li,Xuecheng Zhang,Haixia Lyu,Zenghong Xie
Here, the inner filter effect (IFE) was applied to construct a “turn off” mode up-conversion fluorescence and colorimetric dual-reading detection platform consisting of the chromogenic substance p-phenylenediamine (PPD) and NaYF 4 :Yb/Er@NaYF 4 core–shell up-conversion nanoparticles (UCNPs) for the detection of glucose and hydrogen peroxide (H 2 O 2 ) with high sensitivity and selectivity. In this strategy, hydrogen peroxide is produced in the process of glucose oxidation by catalyzing glucose oxidase (GOx). PPD is oxidized by H 2 O 2 under the action of horseradish peroxidase (HRP), resulting in the transformation into its oxidation product PPDox, whose absorption peak coincides with the emission peak of UCNP at 540 nm, which can significantly quench the fluorescence of UCNP. The quenching efficiency is related to PPDox production, so the relationship between the glucose content and the fluorescence changes of UCNP can be established to realize the sensitive detection of glucose. UCNPs modified by –COOH and the oxidation product of PPD were negatively charged, which increased the distance between donor and recipient due to electrostatic repulsion. It was not conducive to the occurrence of distance-dependent fluorescence resonance energy transfer (FRET), which was also verified by fluorescence lifetime test. Under the optimal experimental conditions, the linear ranges of hydrogen peroxide and glucose were 2.5 ∼ 50 and 5 ∼ 80 μmol/L with the detection limit (LOD) of 0.32 and 0.83 μmol/L, respectively, which were lower than of some reported approaches. Furthermore, the system could be applied to the detection of glucose in actual serum sample and the results were consistency of commercial glucose meters which indicated that the protocol was reliable and potential applications for clinical diagnosis.
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TALANTA
·
2019
·
IF 4.916
Xiao-Dong Sun,Hai-Long Wu,Zhi Liu,Yue Chen,Jun-Chen Chen,Li Cheng,Yu-Jie Ding,Ru-Qin Yu
Chemometrics multivariate calibration coupled with high performance liquid chromatography-diode array detection (HPLC-DAD) analytical strategy was applied for fast and sensitive quantification of the eight small molecules (uric acid, creatinine, tyrosine, homovanillic acid , hippuric acid, indole-3-acetic acid, tryptophan and 2-methylhippuric acid) in human urine. The objective of this work was to get the successful resolution of the complex matrix with minimum experimental time in the presence of highly overlapping peaks, of distortions in the time and baseline aspects among chromatograms, and of the presence of unknown and background interferences. All the analysis were based on a short C18 column with the chromatographic system operating in isocratic mode and all analytes can be successfully quantified within 6 min. The second-order HPLC-DAD data acquired were handled intelligently by two typical chemometrics tools including alternating trilinear decomposition (ATLD) and multivariate curve resolution-alternating least squares (MCR-ALS). Reasonable resolution and satisfactory quantification results were obtained regardless of the complex matrix interferences from the urine samples and the second-order advantage was fully exploited. With the validation by classic HPLC method, the proposed strategy could take extra advantages such as increased selectivity and sensitivity, shorter analysis time, undemanding elution conditions and sufficiency of lower limit of quantification benefit from multivariate calibration. The method was shown as a promising means for fast and sensitive determination of small molecules in human urine and also for fast diagnosis or surveillance in related diseases.
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RSC Advances
·
2019
·Vol. 9
·期 4
·1889-1894
·
IF 3.049
Ying Sun,Hui Liu,Xionghong Tan,Zheng Li,Yanlin Du,Aixian Zheng,Xiaolong Liu,Niancai Peng
Potassium permanganate (KMnO4) is one of the most important oxidants, which plays important roles in many fields. Here, we found that KMnO4 could directly induce the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to generate an oxidized product with a color change. This redox reaction is highly efficient, and 1 μM KMnO4 is enough to cause detectable changes in the absorbance signal. Meanwhile, this reaction is very fast and the generated blue product can stabilize for a relatively long period, which has great advantages in practical applications. Since hydrogen peroxide (H2O2) is able to react with KMnO4 under acidic conditions, the KMnO4-TMB system can be used for the detection of H2O2; the absorbance signal induced by 5 μM H2O2 can be easily detected in this method. Meanwhile, the KMnO4-TMB system can also be used for the detection of glucose by monitoring the generation of H2O2, which is the main product of glucose oxidation; this method permits detection of concentrations as low as 10 μM glucose, and the sensitivity is comparable to or higher than most peroxidase mimetic based methods, but avoiding the preparation and storage of the nanomaterials. Furthermore, the KMnO4-TMB system can even be used for analyzing glucose in serum samples, which can also be expected to be used in immunoassays.
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MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 2
·1-11
·
IF 5.479
Long Ling,Liu Xiangjian,Chen Lulu,Li Dandan,Jia Jianbo
The authors report that CuO x /NiO y hollow nanocomposites are an effective bifunctional catalyst capable of oxidizing glucose and reducing hydrogen peroxide. Synthesis is based on a solvothermal process and subsequent thermal treatment. The structure can be controlled by adjusting the amounts of added NiCl 2 during the solvothermal etching process, and core-shell, yolk-shell or hollow structures can be obtained. The porous hollow structure composite of type CuO 30 /NiO 90 was used to modify a glassy carbon electrode. It exhibits excellent electrocatalytic activity towards glucose oxidation in solution of pH 13, typically at a working potential of +0.60 V (vs. Ag/AgCl). This enables voltammetric sensing of glucose with (a) a low limit of detection (0.08 μM, at S/ N = 3), (b) over a wide linear range (0.20 μM - 2.5 mM), and (c) high sensitivity (2043 μA·mM −1 ·cm −2 ). The sensor is reproducible, selective and stable. It can be used to detect glucose in spiked human serum. The CuO 30 /NiO 90 composite also displays good electrocatalytic activity towards reduction of H 2 O 2 in neutral aqueous medium, typically at an applied potential of −0.35 V. It has a detection limit of 90 nM, a sensitivity of 271.1 μA·mM −1 ·cm −2 , and a linear detection range that extends from 0.30 μM to 9.0 mM.
CuO x /NiO y nanocomposites with three different structures were synthesized by coordinated etching precipitation method. The hollow structure CuO 30 /NiO 90 was coated on the surface of glassy carbon electrode for the amperometric determination of glucose and hydrogen peroxide.
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SENSORS
·
2019
·Vol. 19
·期 5
·1203
·
IF 3.031
Nannan Shen,Haijun Xu,Weichen Zhao,Yongmei Zhao,Xin Zhang
Glucose concentration is an important physiological index, therefore methods for sensitive detection of glucose are important. In this study, Au foam was prepared by electrodeposition with a dynamic gas template on an Au nanoparticle/Si substrate. The Au foam showed ultrasensitivity, high selectivity, and long-term stability in the quantitative detection of glucose. The foam was used as an electrode, and the amperometric response indicated excellent catalytic activity in glucose oxidation, with a linear response across the concentration range 0.5 μM to 12 mM, and a limit of detection of 0.14 μM. High selectivity for interfering molecules at six times the normal level and long-term stability for 30 days were obtained. The results for electrochemical detection with Au foam of glucose in human serum were consistent with those obtained with a sensor based on surface-enhanced Raman spectroscopy and a commercial sensor. This proves that this method can be used with real samples. These results show that Au foam has great potential for use as a non-enzymatic glucose sensor.Keywords:Au foam;bubble-templated electrodeposition;non-enzymatic glucose sensor;amperometric response
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高影响因子
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