CARBOHYDRATE POLYMERS
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2017
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IF 4.811
Jianfang Jiang,Jiaqi Zhao,Chunyang He,Baodong Cui,Jun Xiong,Hao Jiang,Juan Ao,Guangyan Xiang
The use of cellulase hydrolysis of straw to produce fermentable sugars has many application prospects. However, cellulase is very expensive, which hampers its industrial applications. To improve cellulase’s catalytic activity and reduce the enzyme cost, magnetite carboxymethyl chitosan/calcium alginate – cellulase bioconjugate (MCCCB) was synthesized via an improved hydrothermal method, molecular self-assembly technology, physical absorption, embedding and covalent bonding. Its loading capacity was 3.95 mg/mL, and the catalytic activity increased to 267.18%. We decreased the release rate, improved the reusability, and enhanced the stability of MCCCB. Corn stalk hydrolysis also greatly improved, and the overall yield of fermentable sugars increased by 698.26%. All of these results indicate that MCCCB could significantly improve the efficiency of cellulase, greatly reduce the cost of enzyme, and effectively promote the production of fermentable sugars.
研究领域:
生物化学工程
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酶技术
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生物质转化
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可再生能源
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生物催化
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高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Song Chen,Hao Li,Yajia Bai,Jianan Zhang,Toshiyuki Ikoma,Di Huang,Xiaona Li,Weiyi Chen
Biopolymer/hydroxyapatite (HAp) composites are one type of the most promising materials for a variety of biomedical applications. In this study, hierarchical and urchin-like chitosan/HAp nanowire (HU-CS/HAp NW) microspheres were for the first time synthesized by in situ hydrothermal treatment of chitosan/HAp (CS/HAp) microspheres in the acetic acid solution. The results indicate that HU-CS/HAp NW microspheres were spherical in morphology with a diameter of 100–300 μm. Their surface was mainly constructed by numerous HAp NWs with the diameter of 80–120 nm and showed a hierarchical and urchin-like nanofibrous architecture. It was found that the acidic hydrothermal treatment caused an in situ conversion of HAp NPs to HAp NWs. In vitro biocompatible evaluation indicates that HU-CS/HAp NW microspheres showed an enhanced cell attachment and proliferation due to the presence of hierarchical and urchin-like architecture. Furthermore, HU-CS/HAp NW microspheres showed a good adsorption capacity for tetracycline hydrochloride (model drug, one of the most representative antibiotics) with a higher adsorption capacity than CS/HAp microspheres and well maintained their antibacterial efficacy to inhibit the growth of bacteria: Escherichia coli and Staphylococcus aureus . Thus, the present HU-CS/HAp NW microspheres would be applicable as novel drug-laden cell carriers.
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SURFACE & COATINGS TECHNOLOGY
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2023
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IF 5.4
Yanan Wei,Xiaofei Cao,Jun Hu,Neng Li,Li Sun,Weihua Li,Hongwei Liu
The hydration of wettable materials can help to improve their anti-biofouling effect, but their hydration stability under high salinity conditions is a challenge considering the future applications in natural marine environments. Inspired by the self-cleaning properties of crustaceans, chitosan (CS)/Poly (vinyl alcohol) (PVA) hydrogels have been prepared, and their anti-biofouling and salt tolerant mechanism were deeply investigated using surface analysis and molecular dynamics simulation . Interestingly, it is found that CS/PVA hydrogels can be controllably triggered at a low pH, and they can self-adjust to enhance anti-biofouling performance following the changes of environmental conditions. The hydration of CS/PVA hydrogels is enhanced in seawater because of a low salt sensitivity factor ( f ) of these hydrogels with a value of less than 0. Super hydrophilicity of CS/PVA hydrogels makes water drop achieve super spreading within 66.5 ms. Compared to PVA hydrogels, almost no crude oil, protein and algae can adhere to the surface of the prepared CS/PVA (0.2:1) hydrogel. The field tests in the south sea of China also demonstrate that CS/PVA (0.2:1) hydrogel has an excellent anti-biofouling ability after 6 months of testing. The anti-biofouling mechanism is also discussed according to the experimental results and molecular dynamics simulation.
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Biomolecules
·
2023
·Vol. 13
·期 3
·496
·
IF 5.5
Yingyuan Zhao,Zhaoxuan Wang,Shuxian Liu,Shiying Xie,Yinchun Xie,Ruifang Li,Hiroaki Oda
Astaxanthin is a carotenoid with excellent antioxidant activity. However, this small lipid-soluble molecule is insoluble in water and has low stability. Although this situation can be improved when astaxanthin is prepared as a nanosuspension, the aqueous form is still not as convenient and safe as the dry powder form for storage, transport, and use. The lyophilization process provides better protection for thermosensitive materials, but this leads to collapse and agglomeration between nanoparticles. To improve this situation, appropriate lyophilization protectants are needed to offer support between the nanoparticles, such as sugars, amino acids, and hydroxy alcohols. The purpose of this work is to screen lyophilization protectants by single-factor experiments and response surface optimization experiments and then explore the optimal ratio of compound lyophilization protectants, and finally, make excellent astaxanthin/BSA/chitosan nanosuspension (ABC-NPs) lyophilized powder. The work shows that the optimal ratio of the compounding lyophilization protectant is 0.46% oligomeric mannose, 0.44% maltose, and 0.05% sorbitol (w/v). The ABC-NPs lyophilized powder prepared under the above conditions had a re-soluble particle size of 472 nm, with a ratio of 1.32 to the particle size of the sample before lyophilization. The lyophilized powder was all in the form of a pink layer. The sample was fluffy and dissolved entirely within 10 s by shaking with water. Consequently, it is expected to solve the problem of inconvenient storage and transportation of aqueous drugs and to expand the application of nanomedicine powders and tablets.Keywords:lyophilization;astaxanthin nanoparticles;freeze-drying protectant
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Polymers
·
2023
·Vol. 15
·期 7
·1688
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IF 5
Xuemei Bai,Jingmin Luan,Tingting Song,Haifeng Sun,Yuhua Dai,Jianxiang Yu,Huafeng Tian
In this work, a series of chitosan (CS)-grafted carbon oxynitride (OCN) nanoparticles (denoted as CS-OCN) were successfully synthesized for the first time by thermal polycondensation and subsequent esterification. The structure and photocatalytic performance of CS-OCN nanoparticles were investigated. The XPS spectra of CS-OCN-3 showed the presence of amino bonds. The optimal photocatalytic degradation efficiency of the synthesized CS-OCN-3 could reach 94.3% within 390 min, while the photocurrent response intensity was about 150% more than that of pure OCN. The improved photocatalytic performance may be mainly attributed to the enhanced photogenerated carrier’s separation and transportation and stronger visible light response after CS grafting. In addition, the inhibition diameter of CS-OCN-3 reached 23 mm againstE. coliwithin 24 h under visible light irradiation, exhibiting excellent photocatalytic bactericidal ability. The results of bacterial inhibition were supported by absorbance measurements (OD600) studies ofE. coli. In a word, this work provided a rational design of an efficient novel metal-free photocatalyst to remove bacterial contamination and accelerate the degradation of organic dyes.Keywords:carbon oxynitride;CS grafting;MB degradation;antibacterial properties;photocatalytic activity
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Food & Function
·
2023
·Vol. 14
·期 9
·4254-4266
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IF 6.1
Ronggang Liu,Yu Li,Chengfu Zhou,Mingqian Tan
Astaxanthin (AXT) is a lipid-soluble carotenoid with good anti-oxidation, hepatic steatosis reduction, anti-inflammation, and intestinal microbiota regulation ability, whose poor stability and pH vulnerability limit its bioavailability. Spirulina protein (SP) derived from spirulina has good emulsifying ability with potential application in nutraceuticals, medicines, and cosmetics. In this study, Pickering emulsions were prepared using a SP–chitosan (CS) complex as an emulsifier. The particle size, zeta potential, and three-phase contact angle of the SP–CS complex with different SP to CS ratios were investigated. A mass ratio of 1 : 2.5 SP–CS complex showed a good emulsifying ability in preparing Pickering emulsion. A higher storage modulus and viscoelasticity were observed with higher SP–CS complex concentrations and oil fractions. The SP–CS Pickering emulsion significantly improved the stability of AXT in different environments. The lipid release rate and AXT bioavailability after digestion of 3 wt% SP–CS complex-stabilized Pickering emulsion reached 70.54 ± 1.59% and 36.60 ± 3.44%, respectively. The results indicated that the SP–CS complex could act as a Pickering emulsion stabilizer and had the potential to deliver protective hydrophobic AXT.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Shaochun He,Junting Li,Xundan Cao,Fei Xie,Hui Yang,Cheng Wang,Carla Bittencourt,Wenjiang Li
A novel reusable, high-compressible cotton regenerated cellulose/chitosan composite aerogel (RC/CSCA) was prepared using N -methylmorpholine-N-oxide (NMMO) as the green cellulose solvent, and glutaraldehyde (GA) as the crosslinking agent. The regenerated cellulose obtained from cotton pulp could chemically crosslink with chitosan and GA, to form a stable 3D porous structure. The GA played an essential role in preventing shrinkage and preserving the deformation recovery ability of RC/CSCA. Due to the ultralow density (13.92 mg/cm 3 ), thermal stability (above 300 °C), and high porosity (97.36 %), the positively charged RC/CSCA can be used as a novel biocomposite adsorbent for effective and selective removal of toxic anionic dyes from wastewater, showing an excellent adsorption capacity, environmental adaptability, and recyclability. The maximal adsorption capacity and removal efficiency of RC/CSCA for methyl orange (MO) was 742.68 mg/g and 95.83 %.
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LWT-FOOD SCIENCE AND TECHNOLOGY
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2023
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IF 6
Shixin Song,Lu Zhong,Yihua Wei,Yufei Li,Li Tao,Lei Yu
This paper aimed to construct kafirin-chitosan complex particles through the anti-solvent co-precipitation method and explore its potential as a Pickering emulsifier. The results showed that the contact angle of prepared kafirin-chitosan particles was close to 90° and the zeta potential exceeded 50 mV at the mass ratio of 8:1. The highly charged kafirin-chitosan particles with good wettability could effectively adsorb at the oil/water interface. The droplet size of the Pickering emulsion decreased with the increase of kafirin-chitosan particle concentration. When the particle concentration was 2.0 wt% and the oil fraction was 70%, the droplet size of the Pickering emulsion was about 12.4 ± 0.8 μm. Such a small droplet size could increase the droplet-droplet interaction, thus endowing Pickering emulsion with good viscoelasticity. The microstructures indicated that the kafirin-chitosan particles thoroughly covered the polygonal oil droplets, which was conducive to improving the interface film strength of the Pickering emulsion. Consequently, the prepared Pickering emulsion had excellent rheological properties and anti-coalescence stability. The Pickering emulsion at low pH and ionic strength had good stability after 42 days of storage. Therefore, this present research provided a practical strategy to fabricate edible Pickering emulsions with good texture and stability.
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JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
·
2022
·Vol. 111
·期 3
·404-414
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IF 4.854
Xiaoxue Wang,Jingliang Wu,Min Wang,Chunbo Lu,Wenfang Li,Qiao Lu,Yanying Li,Bo Lian,Bo Zhang
Recent efforts have focused on preparing drug-loaded hydrogel for wound healing. In order to obtain an ideal hydrogel dressing for skin wound repair, a carboxymethyl chitosan-gelatin hydrogel was prepared for co-delivery of SP (substance P) and DMOG (dimethyloxallyl glycine) by a chemical cross-linking method using genipin as the cross-linking agent. The synthesized hydrogels have good biocompatibility and physicochemical properties due to the low toxicity of the hydrogel material. The three-dimensional network structure of the hydrogels supports cell migration and proliferation, and the combination of SP and DMOG drugs exhibited strong effects on cell proliferation. Moreover, the co-loaded drug hydrogels could significantly promote wound healing in vivo, and provide a potential hydrogel for wound healing.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Shu Xie,Tianwei Dai,Fei Li,Fengyi Liu,Quanqing Xu,Aixin Zhu,Xufeng Zhang
Hybrid hydrogels containing biomacromolecules have been widely used in sensors, fluorescent probes , and other fields due to their high biocompatibility and nontoxicity. In this paper, tough hydrogels with interconnected macro-pores have been fabricated by freeze-induced chemical cross-linking of microfibrillated cellulose (MFC) and organic modified chitosan (CS). In this hydrogel materials, three-dimensional networks were formed by abundant hydrogen bonds and chemical cross-linking. Luminescent lanthanide complexes were covalently bonded to the hydrogel networks through coordination of Eu 3+ ions with 2, 3-pyridine dicarboxylic acid modified chitosan. The luminescence of hydrogel materials was further improved by the replacement of coordination water with 2-thiophenyltrifluoroacetone (TTA). The prepared hydrogels showed excellent shape memory properties both under water and in air. The stress of the hybrid hydrogel at 80 % strain can reach 159 kPa, which is much higher than that of the traditional microfibrillated cellulose-based hydrogels. The obtained luminescent hybrid hydrogels exhibited an excellent fluorescence detection effect on formaldehyde. The detection limit for formaldehyde is 45.7 ppb, which is much lower than the WHO standard (80 ppb for indoor air). The novel, facile preparing procedure may extend the potential applications of hybrid lanthanide luminescent hydrogel as fluorescence probes for pollution monitoring, especially for formaldehyde and other organic aldehydes.
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LANGMUIR
·
2023
·
IF 3.9
Xingrui Qi,Jiamin Gan,Zhidong Zhao,Nian Li,Yufang Chen,Tao Jin
One of the potential treatments for bacterial wound infections is photodynamic therapy. WO3–x semiconductor materials can generate reactive oxygen species when exposed to light, which can inactivate bacteria. In this work, we improved their photocatalytic performance by doping WO3–x with Cu. The wound dressing was prepared by loading Cu–WO3–x into a highly biocompatible chitosan sponge. The composite sponge dressing showed significant inactivation of Escherichia coli and Staphylococcus aureus, and in vitro toxicity assays on L929 cells demonstrated the biosafety of the dressing. Through in vivo wound healing trials, composite sponge dressings have been shown to accelerate wound healing, and this composite chitosan sponge can be possibly used for photodynamic therapy of bacterial wound infections.
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FOOD HYDROCOLLOIDS
·
2023
·
IF 10.7
Wei Zhang,Pei Gao,Qixing Jiang,Wenshui Xia
Maximizing the waste valorization of renewable resources to develop biocomposite materials is highly desirable regarding environmental sustainability and food chain security. Herein, lignin nanoparticles (LNPs) obtained using deep eutectic solvent (DES) by anti-solvent strategy were employed as nanofillers in the chitosan matrix to fabricate novel bioactive packaging films. The results showed that adding LNPs reduced the swelling degree and water vapor transmittance of chitosan films. Upon introduction of LNPs content, the tensile strength of the composite films significantly increased by about 40%, whereas the elongation at break showed an opposite trend. When the content of LNPs was 10 wt %, the UV shielding and free radical scavenging ability of the composite films reached the maximum point. However, the diameter of the inhibition zone only increased at the addition of 1 wt%. Furthermore, the refrigerated (4 ± 1 °C) grass fish wrapped with composite films revealed better quality and the shelf life was extended to 10 days. Therefore, nanocomposite films provide an attractive and promising pathway for high value-added commercial utilization of lignin and chitosan for food packaging applications.
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危险品化学品经营许可证（带存储）