RSC Advances
·
2019
·Vol. 9
·期 12
·6548-6555
·
IF 3.049
Hui Li,Yang Pang,Xin Wang,Xun Cao,Xun He,Kequan Chen,Ganlu Li,Pingkai Ouyang,Weiming Tan
Methods for enhancing enzyme activities in two-phase systems are getting more attention. Phospholipase D (PLD) was successfully encapsulated into metal-surfactant nanocapsules (MSNCs) using a one-pot self-assembly technique in an aqueous solution. The highest yield for the production of high-value phosphatidylserine (PS) from low-value phosphatidylcholine (PC) in the two-phase system was achieved by encapsulating PLD into MSNCs formed from Ca2+ which gave an enzyme activity that was 133.6% of that of free PLD. The PLD@MSNC transformed the two-phase system into an emulsion phase system and improved the organic solvent tolerance, pH and thermal stabilities as well as the storage stability and reusability of the enzyme. Under optimal conditions, PLD@MSNC generated 91.9% PS over 8 h in the two-phase system, while free PLD generated only 77.5%.
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SYNTHETIC METALS
·
2019
·
IF 2.87
Huihui Gu,Yuancheng Qin,Weili Dai,Dan Zhou,Yu Xie
To explore the effect of fluorine atom on photophysical properties, in this work, we synthesized three new small molecules (SMs) with acceptor-donor-acceptor (A-D-A) configuration, SM-0F , SM-2F and SM-4F , where 4,8-bis(octyloxy)benzo[1,2-b:4,5-b']dithiophene ( BDT ) as the electron-donating and benzo[d][1,2,3]triazole ( BTA ) or fluorinated BTA as electron-withdrawing. The three small molecules exhibit good solubility and thermal stability as well as strong absorption. The fluorination of BTA unit made SM-2F and SM-4F possess a relatively lower the highest occupied molecular orbital (HOMO) and higher electron mobility in comparison with SM-0 F. We treated the three small molecules as donor and PC 71 BM as the acceptor to manufacture small molecule organic solar cells. After introduction of F, the power conversion efficiency (PCE) is improved. Among them, device based on SM-2F gave the best device performance with a PCE of 3.94% with J sc of 11. 37 mA/cm 2 , V oc of 0.76 V, fill factor ( FF ) of 0.45. These studies show that the incorporation of fluorine atom is an effective method to increase the efficiency of organic solar cells.
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JOURNAL OF CHEMICAL AND ENGINEERING DATA
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2019
·
IF 2.298
Xinbao Li,Kaihui Li,Ali Farajtabar,Yating He,Gaoquan Chen,Hongkun Zhao
The solubilities of d-tryptophan in neat n-propanol, ethyl acetate, ethylene glycol (EG), n-butanol, acetonitrile, 1-octanol, and dimethyl sulfoxide and of l-tyrosine in ethyl acetate, EG, isopropanol, acetonitrile, n-butanol, and N,N-dimethylformamide (DMF) were determined via a shake-flask method at temperatures from 283.15 to 328.15 K under about 101.2 kPa. At the fixed temperature, the mole fraction solubility of d-tryptophan was the largest in EG and the lowest in 1-octanol. However, for l-tyrosine, the maximum solubility was observed in the ethyl acetate and minimum in n-butanol. X-ray powder diffraction analysis was carried out to analyze the solids equilibrated with the corresponding liquor, indicating that no polymorphic transformation or solvate formation took place in the studied systems. The solubility correlation was performed via the Apelblat equation. The obtained maximum root-mean-square deviation and relative average deviation values were 13.65 × 10–5 and 4.32%, respectively. In addition, the solvent–solvent and solute–solvent interactions were investigated on the basis of the approach of linear solvation energy relationship.
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ANALYTICA CHIMICA ACTA
·
2019
·
IF 5.256
Jimei Ma,Heng Zhang,Fangfang Peng,Xiaoqing Yang,Zi-Long Li,Linhao Sun,Hong Jiang
Carbon dots (CDs) were synthesized from p -aminophenol and ethylenediamine via one-step under mild condition and used as a sensitive fluorescent nanoprobe for the activity determination of N -acetyl-β- d -glucosaminidase (NAG). In this assay, p -nitrophenol was in situ produced from p -nitrophenyl-β-D- N -acetyl-glucosaminide, which was exclusively hydrolyzed by NAG. The UV absorption peak of p -nitrophenol (maximum at 400 nm) overlapped the excitation peak of CDs with maximum wavelength at 415 nm, which caused the fluorescence decline of CDs based on inner filter effect. The activity of NAG was determined by the fluorescence changes. The assay is highly sensitive to NAG with a low detection limit of 0.75 U L −1 (K = 3) and showed a good linear relationship in the range from 1 to 45 U L −1 . This CDs nanoprobe was successfully applied for the determination of NAG activity in human serum and urine samples.
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ANALYST
·
2018
·Vol. 143
·期 10
·2369-2376
·
IF 3.864
Fan Hu,PinPing Wu,RuYa Wang,Wei Liu,Hua He
Herein, a simple, novel, and rapid colorimetric sensor for cimetidine (Cim) detection based on D-xylose protected gold nanoparticles (D-x@AuNPs) has been developed for the first time. The D-x@AuNPs were characterized by UV-vis, TEM and FT-IR techniques. Cimetidine causes the aggregation of D-x@AuNPs due to the formation of a strong covalent Au–N bond and electrostatic binding. As the cimetidine concentration increased, the color of the solutions gradually changed from wine-red to blue, and the large absorption band shifted from 533 to 680 nm upon D-x@AuNP aggregation. The effects of different experimental parameters were investigated. A comparative study on the detection of Cim using citrated capped AuNPs and D-x@AuNPs was presented. Under optimum conditions, the UV-vis spectra showed that the absorption ratio (A680/A533) increased linearly with the concentration of cimetidine in the range of 7 × 10−8 to 3 × 10−6 M with a correlation coefficient of 0.9956 and a limit of detection of 1 × 10−8 M without needing any complicated instruments. The selectivity of the D-x@AuNP detection system for cimetidine was excellent when compared with other ions and analytes. Due to their rapid and visible color changes, and their remarkable selectivity, the D-x@AuNPs synthesized in this study were suitable and could be applied to the detection of cimetidine in human urine.
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高影响因子
NEW JOURNAL OF CHEMISTRY
·
2018
·Vol. 42
·期 14
·11334-11340
·
IF 3.201
Jingjing Kang,Yakun Tang,Shasha Gao,Lang Liu
In this research, a series of one-dimensional controllable crosslinked polymers with the different mass ratios of 4-vinylbenzychloride (VBC) and divinyl benzene (DVB) was prepared via cationic polymerization, and the polymers were grafted with the functional group N-methyl-D-glucamine (NMDG) for boron removal. The optimum polymeric mass ratio and a series of boron adsorption performances were studied. The maximum adsorption capacity was 18.15 mg g−1, of which 94% was retained after 4 cycles. Meanwhile, different pH values had negligible effect on the boron adsorption capacity, which was favorable for the removal of boron from its aqueous solution in a wide pH window. In addition, a magnetic adsorbent was prepared by the combination of Fe3O4 nanoparticles and the polymer, which provided an effective way to collect and recover adsorbents from their aqueous solutions.
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TALANTA
·
2018
·
IF 4.244
Tianxin Hou,Kui Zhang,Xiaoxuan Kang,Xueling Guo,Libo Du,Xinfeng Chen,Long Yu,Ji Yue,Hongwei Ge,Yang Liu,Abdullah M. Asiri,Khalid A. Alamry,Huan Yu,Suhua Wang
Peroxynitrite is a short-lived endogenous reactive species and plays important roles in many physiological and pathological processes. In this work, we synthesized a near-infrared probe based on the structure of benzothiazole derived cyanine for determination of peroxynitrite (ONOO - ). The designed probe specifically reacted with ONOO - through oxidative cleavage of conjugated C˭C double bonds and generating the non-fluorescent product. Meanwhile, the characteristic absorption of the probe at 630 nm greatly decreased after reaction with ONOO - , accompanied by drastic color change from bright blue to green yellow, which exhibited a distinct visual feature. It was demonstrated that the probe could be used to measure ONOO - in a dose-response manner and had a detection limit lower as 26 nM. Furthermore, the probe Cy-SN was applied for the imaging of endogenous ONOO - in living cells by confocal microscopy , which showed good cell permeability and low cytotoxicity. Successful application of probe for exogenous colorimetric detection and endogenous fluorescence imaging of ONOO - is suggesting its great potential applications in biological analysis.
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FOOD AND CHEMICAL TOXICOLOGY
·
2018
·
IF 3.977
Ying Xiao,Jialin Dong,Zhiting Yin,Qiguo Wu,Yiming Zhou,Xiaoli Zhou
To elucidate the possible mechanisms for the preventive effect of procyanidin B2 on aging, a combined analysis of metabolic profile and gut microbiome was carried out in the present study. The mimetic aged mice induced by d -galactose injection (500 mg/kg, sc daily), and the preventive group was fed with the diet plus 0.2% procyanidin B2. After 7 weeks of treatment, the spatial memory was assayed using the Morris water maze test. Procyanidin B2 significantly ameliorated the impaired memory and antioxidant abilities induced by d -galactose. Furthermore, metabolomics analysis of plasma based on LC/Q-TOF-MS demonstrated that phosphatidyl cholines , oleic acid , linoleic acid , carnitine , pantothenic acid , and taurocholic acid were significantly increased in the mice treated with procyanidin B2, and pyruvic acid , hydroxybutyric acid , hippuric acid , and cholic acid were decreased significantly. Together, gut microbiome analysis using Illumina sequencing showed that there were significant differences in the Firmicutes / Bacteroidetes ratio and abundance of Roseburia , Lachnospiraceae , and Bifidobacterium between the aging and supplemental procyanidin B2 groups. In summary, procyanidin B2 possessed potential prevention of the cognitive and oxidative impairment via the metabolic pathway regulation related to citrate cycle, fatty acid, and bile acid in the aged mice, accompanied by remodeling the gut flora.
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TALANTA
·
2018
·
IF 4.244
Wenya Feng,Juan Qiao,Dan Li,Li Qi
Utilizing block copolymers as coatings, a protocol of chiral ligand exchange capillary electrochromatography (CLE-CEC) protocol was designed and developed with dual ligands for D,L-amino acids enantioseparation. Four block copolymers including poly maleic anhydride- co -styrene- co -N-methacryloyl- L -histidine methyl ester [P(MAn-St-MAH)], poly maleic anhydride- co -styrene- co -N-methacryloyl- L -lysine methyl ester [P(MAn-St-MAL)], poly maleic anhydride- co -styrene- co -N-methacryloyl- L -phenylalanine methyl ester [P(MAn-St-MAP)] and poly maleic anhydride- co -styrene- co -N-methacryloyl- L -threonine methyl ester [P(MAn-St-MAT)] were synthesized by reversible addition fragmentation chain transfer polymerization reaction. Key factors affecting the enantioresolution were optimized, including the concentration of Zn (II) central ion, pH value of buffer solution and monomers of the block copolymers. The enantioresolution of the proposed CLE-CEC system could be enhanced dramatically by employing P(MAn-St-MAH) as the immobilized chiral ligand and by coordinating the synergistic effect of free ligand in buffer solution. The principle of improved enantioresolution of the CLE-CEC system with dual ligands was discussed. Well enantioseparation was successfully realized with 7 pairs of D,L-amino acids enantiomers baseline separation and 5 pairs part separation. For quantitative analysis of D, L -alanine, a good linearity was established in the range of 9.4 μM to 1.5 mM (r 2 = 0.997) with the limits of detection (LODs) 3.7 μM of D -alanine, 2.0 μM for L -alanine, and limits of quantification (LOQs) 9.0 μM for D -alanine and 6.0 μM for L -alanine. The peak area and migration time reproducibility (n = 6) were 4.1% and 3.5% for D -alanine, 3.7% and 3.1% for L -alanine. Further, the enzyme kinetics study of alanine aminotransferase was investigated with the constructed CLE-CEC system.
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APPLIED CATALYSIS A-GENERAL
·
2018
·
IF 4.521
Xiaoqian Guo,Feng Guo,Yishan Li,Zhangqin Zheng,Zhexu Xing,Zihan Zhu,Ting Liu,Xin Zhang,Ying Jin
Dehydration of D-xylose to yield furfural was carried out using bimetallic salts of a heteropolyacid as the catalyst at 160–220 °C in the DMSO/H 2 O mixtures. The effect of Sn/Cs molar ratio of the bimetallic salts of 12-tungstophosphoric acid (PW) obtained by ultrasound-assisted coprecipitation on dehydration of D-xylose was investigated. The resultant catalysts were characterized by X-ray diffraction, N 2 adsorption, field emission scanning electron microscopy and energy dispersive X-ray (EDX). It was found that the Sn-Cs codoped PW catalysts retained the Keggin crystal structure of PW. Sn 0.625 Cs 0.5 PW was the most active catalyst in the dehydration of D-xylose into furfural. The maximum D-xylose conversion (close to 100 wt %) and furfural yield (63 wt %) were achieved at 200 °C for 3 h in DMSO/H 2 O mixtures. It was found that 16.7 wt % Sn 0.625 Cs 0.5 PW on a chitosan-derived support displayed similar catalytic activity to that of Sn 0.625 Cs 0.5 PW and good stability after recycling six times.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2014
·
IF 3.107
Mohamed Reda Zahi,Pingyu Wan,Hao Liang,Qipeng Yuan
d-Limonene organogel-based nanoemulsion was prepared by high-pressure homogenization technology. The organogelator type had a major role on the formation of the formulations, in which stearic acid has given nanoemulsions with the smallest droplet size. The surfactant type and concentration also had an appreciable effect on droplet formation, with Tween 80 giving a mean droplet diameter (d ≈ 112 nm) among a range of non-ionic surfactants (Tween 20, 40, 60, 80, and 85). In addition, high-pressure homogenization conditions played a key role in the nanoemulsion preparation. The stability of d-limonene organogel-based nanoemulsion was also investigated under two different temperatures (4 and 28 °C) through 2 weeks of storage. Results showed a good stability of the formulations, which is maybe due to the incorporation of d-limonene into the organogel prior to homogenization. This study may have a valuable contribution for the design and use of organogel-based nanoemulsion as a delivery system in food.
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MICROCHIMICA ACTA
·
2014
·Vol. 181
·期 11
·1407-1413
·
IF 3.719
Liu Cunwei,Li Baoxin,Xu Chunli
We report on a method for visual differentiation between the D- and L-forms of tryptophan (Trp). It is based on the inherent chirality of citrate-capped silver nanoparticles (AgNPs; ca. 15 nm in diameter) that can be used as chiral selector for D- and L-forms of Trp. On addition of D-Trp to a solution of the AgNPs, a color change from yellow to red can be seen, while no color change is found on addition of L-Trp. The chiral assay can be used to determine the enantiometric excess of D-Trp in the range from −50 % to 100 %. The effect can be detected with bare eyes and quantified by spectrophotometry. Notably, this method does not require any labeling or chiral modification. The method excels by its sensitivity, low cost, good availability of materials, and its simplicity.
The silver nanoparticles can discriminate the absolute configuration of Trp and determine the enantiomeric excess of Trp
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