NANO
·
2020
·
IF 1.566
Zhifeng Cai,Xiu Yin,Jingling Fang,Jie Zhao,Tianqi Wu,Erxiao Hao,Fang Tian,Caifeng Zhang
In this contribution, a one-pot synthesis method possessing the advantages of simple, green and low-cost had been researched for the preparation of L-histidine-stabilized Cu nanoclusters (Cu NCs). Subsequently, the structure and optical properties of as-prepared Cu NCs were studied by using Fourier transform infrared (FTIR), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), fluorescence spectroscopy and UV-Vis absorption spectroscopy. TEM image of the Cu NCs showed high dispersion with an average diameter of 2.0 nm. Fluorescence spectrum displayed that the Cu NCs emitted green fluorescence (emission wavelength of 492 nm) under excitation wavelength of 393 nm. Moreover, the as-synthesized Cu NCs illustrated excellent performances, such as good water solubility, UV stability and high-salt resistance. Interestingly, the fluorescence intensity of as-prepared Cu NCs was obviously quenched in the presence of fluazinam. Under optimal conditions, the relative fluorescence intensity was linear with the fluazinam concentrations from 1 to 40 μ M, with a detection limit of 0.25 μ M. Eventually, the fluorescence sensor was successfully used to determine fluazinam in real water samples.
研究领域:
纳米材料
·
分析化学
·
环境监测
·
荧光传感
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DOI
高影响因子
CHEMISTRY-A EUROPEAN JOURNAL
·
2012
·Vol. 19
·期 2
·501-508
·
IF 5.925
Jianwei Nai,Zhengbo Chen,Haopeng Li,Fangyuan Li,Yang Bai,Lidong Li,Lin Guo
As the properties of nanomaterials are strongly dependent on their size, shape and nanostructures, probing the relations between macro-properties and nanostructures is challenging for nanoscientists. Herein, we deliberately chose three types of Ni(OH) 2 with hexagonal, truncated trigonal, and trigonal hourglass-like nanostructures, respectively, as the electrode modifier to demonstrate the correlation between the nanostructures and their electrocatalytic performance towards L -histidine. It was found that the hexagonal hourglass-like Ni(OH) 2 sample had the best electrocatalytic activity, which can be understood by a cooperative mechanism: on one hand, the hexagonal sample possesses the largest specific surface area and the tidiest nanostructure, resulting in the most orderly packing on the electrode surface; on the other hand, its internal structure with the most stacking faults would generate a lot of unstable protons, leading to an enhanced electronic conductivity. The findings are important because they provide a clue for materials design and engineering to meet a specific requirement for electrocatalysis of L -histidine, possibly even for other biomolecules. In addition, the hexagonal Ni(OH) 2 -based biosensor shows excellent sensitivity and selectivity in the determination of L -histidine and offers a promising feature for the analytical application in real biological samples. Graphical Structure-dependent performance : Three different types of Ni(OH) 2 with hexagonal, truncated trigonal, and trigonal hourglass-like nanostructures, respectively, were deliberately chosen as electrode modifiers to demonstrate the correlation between the structures and their electrocatalytic performance toward L -histidine (see figure). The electrocatalytic activity of these unique nanostructures depends not only their external architecture, but also on their relevant internal degree of crystallinity.
研究领域:
纳米材料
·
电化学
·
催化
·
生物传感器
·
材料科学
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DOI
高影响因子
JOURNAL OF SOLID STATE ELECTROCHEMISTRY
·
2015
·Vol. 19
·期 10
·3185-3193
·
IF 2.446
Li Yong-Fang,Lv Jing-Jing,Wang Ai-Jun,Zhang Ming,Wang Rui-Zhi,Feng Jiu-Ju
Shape and size control of gold (Au) nanocrystals plays important roles in their catalytic and optical properties. Hierarchical Au nanodendrites were directly electrodeposited on a glassy carbon electrode (GCE) using l -arginine as a growth-directing agent, while there is no seed, template, or surfactant involved. It was found that the experimental conditions such as the applied potentials, concentrations of HAuCl 4 and l -arginine, and deposition time were essential to the final morphology of the Au deposits. The as-synthesized Au nanocrystals displayed enhanced electrocatalytic activity and improved stability for ethylene glycol (EG) oxidation in acidic media as compared to Au nanoparticles.
研究领域:
纳米材料
·
电化学
·
催化
·
材料科学
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DOI
高影响因子
Frontiers in Plant Science
·
2016
·
IF 4.495
Gaofeng Zhou,Sue Broughton,Xiao-Qi Zhang,Yanling Ma,Meixue Zhou,Chengdao Li
Genome-wide association studies (GWAS) based on linkage disequilibrium (LD) have been used to detect QTLs underlying complex traits in major crops. In this study, we collected 218 barley (Hordeum vulgare L.) lines including wild barley and cultivated barley from China, Canada, Australia, and Europe. A total of 408 polymorphic markers were used for population structure and LD analysis. GWAS for acid soil resistance were performed on the population using a general linkage model (GLM) and a mixed linkage model (MLM), respectively. A total of 22 QTLs (quantitative trait loci) were detected with the GLM and MLM analyses. Two QTLs, close to markers bPb-1959 (133.1 cM) and bPb-8013 (86.7 cM), localized on chromosome 1H and 4H respectively, were consistently detected in two different trials with both the GLM and MLM analyses. Furthermore, bPb-8013, the closest marker to the major Al3+ resistance gene HvAACT1 in barley, was identified to be QTL5. The QTLs could be used in marker-assisted selection to identify and pyramid different loci for improved acid soil resistance in barley.
研究领域:
植物遗传学
·
作物科学
·
基因组学
·
非生物胁迫抗性
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DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2026
·
IF 6.4
Lina Chen,Meijia Li,Mengjuan Gong,Mingzhe Yang,Xiaohui Sheng
Different extraction methods alter structural features and bioactivities of American ginseng polysaccharides, supporting their high‑efficiency utilization. • Structure‑activity relationships reveal antioxidant, hypoglycemic and hypolipidemic effects depend jointly on monosaccharide composition, molecular weight and spatial conformation. • Comprehensive characterization of higher‑order structures provides theoretical support for targeted modification of polysaccharides in functional food and pharmaceutical fields. This study systematically investigated the structure–activity relationships of six polysaccharide fractions, aiming to elucidate the intrinsic correlations between their structural characteristics and biological activities. The results revealed that the antioxidant, hypoglycemic, and hypolipidemic activities of the polysaccharides were governed not by any single structural factor, but rather by the synergistic effects of monosaccharide composition, molecular weight distribution, and spatial conformation. Among the fractions, ULPP exhibited the most potent broad-spectrum antioxidant and enzyme inhibitory activities through dual chemical and physical mechanisms, suggesting its potential for multi-target intervention. Although DEPP showed weak antioxidant activity, its unique fibrous-lamellar network structure conferred outstanding physical barrier effects and sodium cholate adsorption capacity, indicating distinctive advantages in lipid metabolism regulation. ENPP demonstrated specific inhibitory activity against α-glucosidase, and structural analysis suggested that this activity was closely associated with its specific monosaccharide composition and branching characteristics. In contrast, HWPP and UPPP displayed poor overall activity, likely due to pronounced structural aggregation that limited the exposure of functional groups. These findings lay a theoretical foundation for targeted structural modification of polysaccharides, emphasize the importance of high-order structures in functional assessment, and guide the rational design and development of functional foods.
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DOI
高影响因子
ACS Synthetic Biology
·
2026
·
IF 4.5
Qiaoqiao Dai,Jiaqi Xu,Xiaozhe Wu,Xiaomin Li,Cong Gao,Guipeng Hu,Liming Liu
As an essential precursor for numerous high-value chemicals, l-tyrosine is in high demand across the food, medical, and chemical sectors. While microbial cell factories offer a green and sustainable route for its production, their efficiency is often constrained by the inherent complexity of metabolic pathways and the carbon flux competition between cell growth and product synthesis. Building upon a previously established l-tryptophan-producing chassis, we fine-tuned the metabolic flux upstream and downstream of the shikimate node and implemented a cofactor recycling-driven strategy, thereby increasing the l-tyrosine titer from 2.5 to 8.6 g/L in shake-flask fermentation (a 3.4-fold improvement). To address the growth-production trade-off, a quorum-sensing-based dynamic control strategy was employed to regulate the expression of the TCA cycle gene sucA. Further integration of screening and reinforcement of glutamate dehydrogenase enabled the effective spatiotemporal decoupling of cell growth from l-tyrosine biosynthesis. Consequently, the engineered strain achieved an l-tyrosine titer of 81.3 g/L in a 5 L bioreactor, with a record-breaking cell-specific productivity of 0.048 g/g of DCW/h, representing a significant enhancement in the spatiotemporal productivity of microbial l-tyrosine biosynthesis. This study provides a robust strategy for the efficient and sustainable biomanufacturing of aromatic amino acids and their derivatives.
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DOI
PubMed
高影响因子
SOUTH AFRICAN JOURNAL OF BOTANY
·
2024
·
IF 2.7
Kainat Bilal,Fatima Mehboob,Nosheen Akhtar,Irfan Ali Mirza,Muhammad K. Okla,M. Junaid Dar,Ibrahim A. Saleh,Naser Zomot,Humaira Fatima
The current study aimed to evaluate the in vivo wound healing potential of Psidium guajava L. by wound excision model owing to its ethnomedicinal use as a wound healer since olden times. P. guajava leaves were extracted using methanol-chloroform (1:1). Phytochemical analysis of P. guajava leaves extract (PGLE) was performed using total phenolic content (TPC) assay, total flavonoid content (TFC) assays, and High-performance liquid chromatography (HPLC). In vitro , antioxidant potential (DPPH radical scavenging assay, total antioxidant capacity (TAC) assay, and ferric reducing antioxidant power (FRAP) assay), antibacterial activity (broth microdilution method), and hemolytic analysis of PGLE were performed. In vivo , wound healing proficiency of PGLE (10, 5, and 2.5% w/w) was determined by estimating hydroxyproline and endogenous antioxidant markers. TPC and TFC were found to be 57.5 ± 0.37 µg gallic acid equivalent/mg extract and 32.1 ± 0.24 µg quercetin equivalent/mg extract, respectively. A prominent concentration of polyphenols was demonstrated by HPLC-based quantification with the highest value of rutin which is 12.3 ± 0.12 µg/mg extract. A noteworthy DPPH radical scavenging activity with an IC 50 of 40.7 µg/ml, TAC, and FRAP i.e., 159.3 ± 0.11 and 206.1 ± 0.17 µg ascorbic acid equivalent/mg extract, respectively were exhibited by PGLE. Significant antibacterial activity against Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeroginosa, and Methicillin-resistant Staphylococcus aureus with the minimum inhibitory concentration of 250, 400, 400, and 500 µg/ml respectively was depicted by PGLE and found biocompatible with 1.1 ± 0.16% hemolysis. Significant wound contraction, faster epithelialization (9 ± 1 days), extensive fibroblast proliferation, and substantial neovascularization were observed in the 10% PGLE-treated group also evident by histopathological examination. Significant hydroxyproline (15.7 ± 1.02 µg/mg tissue), catalase, peroxidase (5.4 ± 0.26 and 6.1 ± 0.35 U/mg tissue) and glutathione content (9.7 ± 0.06 mmol/mg tissue) with p-value of less than 0.01 was demonstrated by 10% PGLE. A reduction in lipid peroxidation (0.5 ± 0.07 mmol/mg tissue) was also observed at the same concentration (10%). These findings scientifically validate the potent pharmacological effects of P. guajava phytochemicals and suggest their promising role as wound healing agents.
研究领域:
药理学
·
植物化学
·
伤口修复
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DOI
高影响因子
Journal of Agriculture and Food Research
·
2025
·
IF 6.2
Ziyang Wang,Qinqin Ouyang,Xiaolin Liang,Hongxia Li,Haiying Guo,Rong Liu
Traditional Chinese medicine polysaccharides (TCMPs) have attracted growing attention due to their multi-target and multi-level therapeutic effects in the treatment of diabetes. In this study, we systematically evaluated the antidiabetic effect of three TCMPs, including Moringa oleifera Lam. polysaccharide (MOP), Morus alba L. polysaccharide (MLP) and Lycium barbarum polysaccharide (LBP) in T2DM mouse model. Among the three, MLP exhibited the most pronounced therapeutic efficacy, markedly alleviating hyperglycemia, insulin resistance, hepatic steatosis, and pathological lesions in the liver, pancreas. Liver metabolomic profiling revealed that MLP significantly modulated key glucose and lipid metabolic pathways, particularly bile acid and glycerolipid metabolism. Furthermore, we found MLP exerted its antidiabetic action through inhibition of the mTORC1 signaling pathway, leading to suppressed lipogenesis and enhanced fatty acid oxidation. Notably, MLP-mediated mTORC1 inhibition activated the PPARα–SIRT1 axis, thereby downregulating FOXO1-driven gluconeogenesis and promoting GLUT4-mediated glucose uptake. These findings suggest that MLP exerts comprehensive antidiabetic effects by targeting mTORC1-mediated metabolic reprogramming, offering promise as a natural therapeutic agent for T2DM.
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产品关联
DOI
高影响因子
Foods
·
2025
·Vol. 14
·期 24
·4286
·
IF 5.1
Wei Li,Xinyao Han,Wei Wang,Qingping Du,Mingxun Ai,Shihao Huang,Tongle Sun,Hongji Zeng,Yuhang Li
The present research sought to investigate the impacts of Brassica rapa L. polysaccharide (Brp) on glycolipid metabolism in diabetic rats and the regulatory role of gut microbiota in metabolic balance. After 30-day Brp gavage, glycolipid metabolic parameters and amino acid levels were measured, and gut microbial communities were sequenced and analyzed. The results showed that Brp improved glycolipid metabolism, alleviated insulin resistance and hepatic oxidative stress, increased liver glycogen synthesis, and modulated gut microbiota composition. Specifically, Brp potentially enhanced short-chain fatty acid (SCFAs) production by enriching Blautia and Roseburia populations while reducing lipopolysaccharide (LPS)-producing bacteria to lower pathological risks. Notably, Brp may reduce the risk of T2D by increasing the concentration of lysophosphatidic acid (18:2(9Z,12Z)/0:0), indoleacrylic acid, cholic acid, and betaine, and decreasing high-risk metabolites such as 3,4-dihydroxybutyrate, xanthine, and carnitine, as well as regulating branched-chain amino acids and aromatic amino acids throughout the development of T2D.
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DOI
PubMed
高影响因子
MACROMOLECULES
·
2025
·
IF 5.2
Shangzhong Zhang,Yixuan Xu,Panpan Li,Minghui Fan,Dejia Chen,Chengming Wang,Lifeng Yan
Polymerization of CO2 into polyurea under mild pressure and temperature is still a challenge, especially when using diamine from natural feedstocks. Herein, l-lysine is directly used as a diamine without derivatization and protection for capture and copolymerization of CO2 under mild temperatures and pressures (0.1–0.5 MPa, 60–90 °C) with the assistance of superbase 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU). Water-soluble lysine-polyurea (L-PU) has been synthesized. The weight-average molecular weight of L-PU reaches 3 × 104 g/mol, indicating the efficient reaction of lysine and CO2. In addition, NMR studies confirmed that the unprotected carboxyl acid group (−COOH) in L-PU remains more than 80%, and the polymer may be a candidate to replace nonbiodegradable polyacrylate acid (PAA). The free carboxyl acid group also provides a change to further derivatives of the L-PU. Interestingly, the water-soluble L-PU showed efficient fluorescence performance with quantum yield (QY) up to 20%, which may be attributed to the nonconventional intrinsic fluorescence of nonconjugated aliphatic polyureas containing heteroatoms. It allows the L-PU to be used for cell bioimaging, and in vitro studies reveal that the L-PU is biocompatible and can be efficiently endocytosed by cells with bioimaging ability, indicating that it may be a potential drug delivery system for imaging-guided treatment for cancer tissue.
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产品关联
DOI
高影响因子
Frontiers in Physiology
·
2020
·
IF 3.367
Li Fan,Li Ke,Wu Li-Juan,Fan Yong-Liang,Liu Tong-Xian
Oviposition is an important reproductive behavior that is triggered by mating in insects, and biogenic amines might be involved in its regulation. The effects of biogenic amines on oviposition have only been studied in a few insect species, and the findings to date have not been conclusive. In addition, there are few studies on the effects of biogenic amines on oviposition of the diamondback moth, Plutella xylostella L. Here, we tested how mating and biogenic amines regulate oviposition of P. xylostella by injecting amines and amine receptor antagonists into virgin and mated females and counting the number of eggs laid afterward. Biogenic amines of octopamine and tyramine could induce virgin adults of P. xylostella to lay eggs, while dopamine and serotonin had no such effect on oviposition. Furthermore, the octopamine antagonists mianserin, epinastine, and phentolamine inhibited oviposition by mated females. The tyramine antagonist yohimbine, dopamine antagonist SCH23390, and serotonin antagonist ketanserin did not block oviposition by mated females, and octopamine and tyramine-inducing oviposition by virgin females could be inhibited by the octopamine antagonists mianserin and epinastine instead of the tyramine antagonist yohimbine. We conclude that octopamine and its receptors are involved in mating-triggered oviposition in P. xylostella, while tyramine acts as a subsidiary. Further, the inducing effect of tyramine on oviposition is achieved via octopamine receptors instead of tyramine receptors. This experiment is helpful to further understand the role of biogenic amines in mating regulation and to provide a new strategy for controlling P. xylostella.
研究领域:
昆虫学
·
神经生物学
✓ 已验证
DOI
PubMed
高影响因子
MATERIALS CHEMISTRY AND PHYSICS
·
2019
·
IF 2.781
Mengjue Cao,Yi Feng,Qian Chen,Pengcheng Zhang,Shihang Guo,Jianfeng Yao
In this study, melamine sponge (MS) is modified by in-situ growth of leaf-like Co-ZIF-L without the addition of additives or surfactants . Such modified MS shows good underwater superoleophobicity because of the unique pore structure and highly rough surface of MS@Co-ZIF-L. Compared to the pristine MS, the water-wetted MS@Co-ZIF-L exhibits excellent water-oil separation for various oils (the separation efficiencies are higher than 99.2%) with good long-term stability (no decrease in flux and separation efficiency after 60 cycles of separation). Moreover, MS@Co-ZIF-L is highly effective to treat oil-water emulsions. Besides the excellent separation performance, due to the outstanding mechanical properties, MS@Co-ZIF-L can be shaped into various configurations such as membranes, filters, adsorbing foams or other forms for the oil/water separation in various situations.
研究领域:
材料科学
·
环境科学
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DOI
高影响因子
危险品化学品经营许可证（带存储）