BIOMATERIALS
·
2017
·
IF 8.402
Fei Duan,Xiaochen Feng,Xinjian Yang,Wentong Sun,Yi Jin,Huifang Liu,Kun Ge,Zhenhua Li,Jinchao Zhang
Tumor-associated antigens (TAAs)-loaded nanoparticles are able to be actively internalized by antigen-presenting cells (APCs) and have shown promising potential in cancer immunotherapy . However, current TAAs delivery strategy exhibits limitations of complicated synthesis process, low loading efficiency and inefficient CD8 + cytotoxic T lymphocyte activation leading to unsatisfactory therapeutic effect. Thus, the construction of novel TAAs-delivery systems for enhanced cancer therapy is highly desirable. In this work, we fabricated a very simple yet powerful antigens-delivery system for cancer immunotherapy based-on pH-responsive metal-organic frameworks (MOFs) with size about 30 nm. TAAs can be loaded into MOFs in the one-pot synthesis process and released with the degradation of MOFs in the acidic environment of endo/lysosome as the result of relatively labile metal-ligand bonds. The endosomolytic nanoparticles would facilitate protein antigens escape from endo/lysosome and optimal for enhancing antigen cross-presentation. Furthermore, the introduction of immunostimulatory unmethylated cytosine-phosphate-guanine oligonucleotide (CpG) through Watson−Crick base pairing would further enhance CD8 + cytotoxic T lymphocyte responses. We demonstrated that the method to co-delivery antigens and immunostimulatory molecules was very simple, convenient and effective and showed no obvious toxicity both in vitro and in vivo . This method gave a high antigens-loading capacity and the maximal antigen encapsulating efficiency was about 55% (w/w). Additionally, the pH-responsive co-delivery system exerted enhanced antitumor outcome (about 100% survival) in B16-OVA melanoma cancers in vivo . Furthermore, we confirmed that this high rating of therapeutic effect was attributed to the recruitment of tumor-killing immunocyte. This work demonstrates the ability of pH-responsive, endosomolytic MOFs to induce strong cellular immune responses for cancer therapy by co-delivery of CpG ODN and antigens.
研究领域:
生物医学工程
·
免疫学
·
癌症研究
·
纳米技术
✓ 已验证
DOI
PubMed
高影响因子
LANGMUIR
·
2021
·
IF 3.882
Yimin Zhou,Cong Chang,Zuhao Liu,Qiuling Zhao,Qingni Xu,Chaohua Li,Yuqi Chen,Yueli Zhang,Bo Lu
Hollow mesoporous silica nanoparticles (HMSNs) served as nanocarriers for transporting doxorubicin hydrochloride (DOX) and indocyanine green (ICG) and were incorporated into a pH-sensitive targeted drug delivery system (DDS). Boronate ester bonds were employed to link HMSNs and dopamine-modified hyaluronic acid (DA-HA), which acted as both the “gatekeeper” and targeting agents (HMSNs-B-HA). Well-dispersed HMSNs-B-HA with a diameter of about 170 nm was successfully constructed. The conclusion was drawn from the in vitro drug release experiment that ICG and DOX (ID) co-loaded nanoparticles (ID@HMSNs-B-HA) with high drug loading efficiency could sustain drug release under acidic conditions. More importantly, in vitro cell experiments perfectly showed that ID@HMSNs-B-HA could well inhibit murine mammary carcinoma (4T1) cells via chemotherapy combined with photodynamic therapy and accurately target 4 T1 cells. In summary, all test results sufficiently demonstrated that the prepared ID@HMSNs-B-HA was a promising nano-DDS for cancer photodynamic combined with chemotherapy.
研究领域:
纳米技术
·
癌症研究
·
药学科学
·
材料科学
✓ 已验证
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2023
·
IF 6
Yang Guo,Zhi-guo Zhang,Wei-wei Hu,Wei-rong Li,Jian-ming Zhang,Cheng-cheng Zhang,Da-qun Liu,Wei-cheng Wu
The acid sensitivity and moisture absorption of dry probiotic products during digestion and storage are challenges. In this study, different mass ratios (1, 1.5, 2, 2.5, 5) of buckwheat protein (BWP) and hydrophobic polyester shellac were firstly combined by a pH-shifting method and then applied for the construction of Lactobacillus plantarum ( L. plantarum ) microcapsules. Circular dichroism (CD) and fluorescence spectroscopy results revealed that the complexes were stabilized primarily by hydrophobic interaction forces and the conformational changes after co-folding with shellac. With a decreasing ratio of shellac, the enthalpy (ΔH) and peak temperature increased, resulting in enhanced thermal stability. BS2 encapsulated probiotics successfully enhances the survival of L. plantarum during freeze-drying and gastrointestinal (GI) digestion. The probiotic was fully coated by the complex, determined through scanning electron microscopy (SEM) images. After encapsulated, the colon adhesion efficiency enhanced 30.92% than free cells. The encapsulated probiotics presented a maximum reduction of 0.89 log CFU/mL after 30 days of storage at 25 °C, and probiotics protected by the BS1 complex had superior storage stability under an Aw of 0.76, for which K value was 1.218. BWP-based shellac pH-shift complexes exhibit enormous potential for the encapsulation of probiotics.
研究领域:
食品科学
·
微生物学
·
生物技术
·
药学
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2017
·
IF 3.649
Yanzhuo Zhang,Wufu Zhu,Heran Zhang,Jin Han,Lihua Zhang,Qisi Lin,Fengwei Ai
In this article, a new type of carboxymethyl chitosan/phospholipid bilayer-capped mesoporous carbon nanomatrix (CCS/PL/MC) was fabricated as a potential nano-drug delivery system. In this drug delivery system, a mesoporous carbon nanomatrix (MC) acts as the support for loading drug molecules, a positively charged phospholipid (PL) layer works as the inner shell for prolonged drug release and a negatively charged carboxymethyl chitosan (CCS) layer serves as the outer shell for pH-responsive drug release. Docetaxel (DTX) was selected as a model drug. The drug-loaded CCS/PL/MC was synthesized via a combination approach of double emulsion/solvent evaporation followed by lyophilization. The drug-loaded nanoparticles were characterized for their particle size, structure, morphology, zeta (ζ)-potential, specific surface area, porosity, drug loading and solid state. In vitro drug release tests showed that the drug-loaded CCS/PL/MC nanoparticles possess a good pH-sensitivity and prolonged releasing ability with negligible release in gastric media and controlled release in intestinal media. Compared with MC and PL-capped MC, CCS/PL/MC had a greater mucoadhesiveness. Moreover, cellular uptake study indicated that CCS/PL/MC might improve intracellular drug delivery. These results suggest that this hybrid nanocarrier, combining the beneficial features of CCS, PL and MC, is a promising drug delivery system able to improve the oral absorption of antitumor drugs.
研究领域:
药剂学
·
纳米医学
·
肿瘤学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF ENVIRONMENTAL ENGINEERING
·
2020
·Vol. 146
·期 12
·04020134
·
IF 1.264
Mahdi Hassan,Guangcan Zhu,Zhonglian Yang,Yongze Lu
A three-dimensional bioelectrochemical reactor (3D-BER) system combining heterotrophic and autotrophic denitrification processes was developed, which possesses the effects of pH on nitrate or nitrite removal and simultaneously elimination of antibiotic sulfamethoxazole (SMX) from simulated municipal wastewater. The experimental results demonstrated that the variation of microbial communities was examined with electric current from 0 to 90 mA at pH 6.50±0.12 to 7.9±0.04. When the current increased from 0 to 90 mA, the nitrate removal efficiency increased from 59.05% to 99.21%, and SMX removal efficiency of 43.9%–93.9% was achieved. The optimum pH of this reactor was 7.0±0.20 to 7.50±0.31, and a lower or higher pH condition may effect autotrophic and heterotrophic denitrification processes. Simultaneously, the denitrification mechanism in this system was analyzed through pH variation in the effluent. The CO2 produced from the anode acted as a good pH buffer, automatically controlling pH in the reaction zone. A low current intensity does not effect the degradation by-products but increases the SMX removal efficacy. Bacterial community analysis revealed substantial changes in microbial communities at the phylum, class, and genus levels after SMX acclimatization. Thauera, a well-known aromatic-degrading bacteria, was the most dominant genus present in acclimatized conditions at 60 mA. In summary, this study shows that high currents changed the microbial structure, and the acclimatized sludge can play a vital role in the biodegradation of SMX, and removal of NO3−─ N from simulated municipal wastewater by a novel 3D-BER.
研究领域:
环境工程
·
水处理
·
生物电化学系统
·
废水修复
·
微生物生态学
✓ 已验证
DOI
高影响因子
JOURNAL OF BIOMATERIALS APPLICATIONS
·
2020
·
IF 2.22
Ke Ma,Yongbin Cheng,Xinran Wei,Daijun Chen,Xiaoli Zhao,Pengxiang Jia
In this work, gold embedded chitosan nanoparticles (Au@CS NPs) were fabricated by a one-pot method. The benzaldehyde-terminated poly[(2-methacryloyloxy) ethyl phosphorylcholine] (PMPC) was applied to modification of the gold doped chitosan nanoparticles. The obtained Au@CS-PMPC NPs had the diameter of 135 nm with a narrow distribution. The size of the Au@CS-PMPC NPs, as well as the size of the embedded gold NPs, might be well-controlled by adjusting the feeding ratio between chitosan and HAuCl4. Furthermore, the Au@CS-PMPC NPs showed increased colloidal stability, high drug loading content, pH-responsive drug release, excellent biocompatibility and bright fluorescence emission. The results demonstrated that Au@CS-PMPC NPs showed a great potential for tumor therapy via the combination advantages of pH-sensitive controlled drug release and cellular fluorescence imaging.
研究领域:
生物医学工程
·
纳米医学
·
药剂学
✓ 已验证
DOI
PubMed
高影响因子
TALANTA
·
2016
·
IF 4.035
Bei Hua Kang,Zhong Feng Gao,Na Li,Yan Shi,Nian Bing Li,Hong Qun Luo
Silver ions have been widely applied to many fields and have harmful effects on environments and human health. Herein, a label-free optical sensor for Ag + detection is constructed based on thiazole orange (TO) as a fluorescent probe for the recognition of i-motif DNA structure change at neutral pH. Ag + can fold a C-rich single stranded DNA sequence into i-motif DNA structure at neutral pH and that folding is reversible by chelation with cysteine (Cys). The DNA folding process can be indicated by the fluorescence change of TO, which is non-fluorescent in free molecule state and emits strong fluorescence after the incorporation with i-motif DNA. Thus, a rapid, sensitive, and selective method for the detection of Ag + and Cys is developed with a detection limit of 17 and 280 nM, respectively. It is worth noting that the mechanism underlying the increase of the fluorescence of thiazole orange in the presence of i-motif structure is explained. Moreover, a fluorescent DNA logic gate is successfully designed based on the Ag + /Cys-mediated reversible fluorescence changes. The proposed detection strategy is label-free and economical. In addition, this system shows a great promise for i-motif/TO complex to analyze Ag + in the real samples.
研究领域:
分析化学
·
生物化学
·
分子生物学
·
环境科学
✓ 已验证
DOI
PubMed
高影响因子
Chemistry-An Asian Journal
·
2015
·Vol. 10
·期 11
·2503-2511
·
IF 4.587
Chao Qi,Ying-Jie Zhu,Tuan-Wei Sun,Jin Wu,Feng Chen
Graphical Amorphous calcium phosphate mesoporous microspheres (ACP-MSs) have been synthesized using adenosine 5′-diphosphate disodium salt as an organic phosphorus source by a rapid microwave-assisted hydrothermal method. The ACP-MSs drug delivery system shows a pH-responsive drug release behavior and a high ability to damage cancer cells after loading doxorubicin. Herein we report a rapid and green strategy for the preparation of amorphous calcium phosphate mesoporous microspheres (ACP-MSs) using adenosine 5′-diphosphate disodium salt (ADP) as an organic phosphorus source by a microwave-assisted hydrothermal method. The effects of the pH value, the reaction time, and temperature on the crystal phase and morphology of the product are investigated. The ADP biomolecules used in this strategy play an important role in the formation of ACP-MSs. The as-prepared ACP-MSs are efficient for anticancer drug delivery by using doxorubicin (Dox) as a model drug, and the Dox-loaded ACP-MSs show a high ability to damage cancer cells. Moreover, the ACP-MSs drug delivery system exhibits a pH-responsive drug-release behavior due to the degradation of ACP-MSs at a low pH value, thus, it is promising for applications in pH-responsive drug delivery.
研究领域:
材料科学
·
生物医学工程
·
药学
✓ 已验证
DOI
高影响因子
JOURNAL OF MATERIALS SCIENCE
·
2016
·Vol. 51
·期 14
·6583-6589
·
IF 2.302
He Enfang,Wang Jianmin,Xu Hai,He Zemin,Wang Hailong,Zhao Hang,Zhang Yingying,Zhang Ruijun,Zhang Haiquan
Graphene oxide/reduced perylene diimide radical anion hybrid (GO/rSFPDI) with high dispersibility in polar organic solvents has been flexibly developed using N , N ′-diethylhexyl-1-bromo-7-pentafluorophenox-perylene diimide (SFPDI). The interaction of the GO with the SFPDI suggested the catalysis of perylene diimide (SFPDI) to reduced perylene diimide radical anion (rSFPDI). The successful preparation of GO/rSFPDI was confirmed via FT-IR, Raman, UV–Vis absorption spectrum, XRD, and SEM. Compared to the two neutral SFPDI and GO, GO/rSFPDI exhibited a novel lower energy electronic transition (max. at 700 nm) in addition to the solvatochromism and characteristics of spectral response to pH. Moreover, the fluorescence emission spectrum of GO/rSFPDI shows strong fluorescence quenching in dilute solution.
研究领域:
材料化学
·
纳米科学
·
物理化学
·
分析化学
·
功能材料
✓ 已验证
DOI
高影响因子
ChemElectroChem
·
2016
·Vol. 4
·期 2
·340-344
·
IF 3.506
Yan Li,Pinwwei Cai,Suqin Ci,Zhenhai Wen
Graphical Hydrogen evolution catalyst : Nanohybrids of Ni 2 P nanofilms on carbon nanosheets show high catalytic activity and excellent stability for the hydrogen evolution reaction in pH-universal electrolyte. A reliable strategy has been developed to prepare Ni 2 P nanofilms on carbon nanosheets (CNS) nanohybrids (Ni 2 P/CNS), which involves the preparation of three-dimensional (3D) CNS followed by in situ growth of Ni 2 P nanofilms on the surface of CNS. The Ni 2 P/CNS nanohybrids possess a robust 3D hierarchical structure, in which the Ni 2 P films are solidly overlaid on the surface of the CNS. The Ni 2 P/CNS nanohybrid was characterized by a variety of techniques, including X-ray diffraction (XRD), electron microscopy, X-ray photoelectron spectroscopy (XPS), etc. A series of electrochemical tests demonstrate the Ni 2 P/CNS nanohybrid shows high catalytic activity and excellent stability towards the hydrogen evolution reaction (HER) in pH-universal electrolyte, that is, in acidic, neutral, and basic media. The outstanding performance for Ni 2 P/CNS is correlated with the synergic effects between Ni 2 P and CNS associated with its unique and robust structure.
研究领域:
材料科学
·
纳米技术
·
电化学
·
能量转换
·
催化
✓ 已验证
DOI
高影响因子
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
·
2020
·
IF 2.69
Zhentao Lei,Qiuju Tang,Yanshan Ju,Yonghui Lin,Xiaowen Bai,Haipeng Luo,Zaizai Tong
Developing the hybrid nanosystems for controlled drug release is still a challenging task. In this work, pH-responsive core-shell nanocomposites have been prepared by the growth of zeolitic imidazolate framework-8 (ZIF-8) on the surface of polymeric aggregates self-assembled from poly(ε-caprolactone)-block-poly (quaternized vinylbenzyl chloride/bipyridine) (PCL-b-q(PVBC/BPy), BCP for short) in water. The core of the micelles or the inner cavity of vesicles serves as the drug storage reservoir for the doxorubicin hydrochloride (DOX) and the ZIF-8 shells act as the gatekeepers to prevent drug premature release at physiological environment. Upon pH stimulus, the core-shell nanocomposites (BCP@ZIF-8) show a retarded drug release behavior compared with DOX-loaded polymeric aggregates counterparts (without the shell of ZIF-8). Moreover, the as-prepared nanocomposites perform good biocompatibility towards MCF-7 cell. Meanwhile, the DOX-loaded BCP@ZIF-8 nanocomposites present lower cytotoxicity compared with DOX-loaded BCP and free DOX. The confocal microscopy study shows the core-shell nanocomposites could be efficiently internalized by cancer cells, and the loaded DOX could be successfully released under acidic intracellular environment. The above result shows that the core-shell nanocomposite could be a promising candidate for pH-responsive drug delivery system in the cancer therapy.
研究领域:
材料科学
·
纳米技术
·
药物递送系统
·
生物医学工程
·
癌症治疗
✓ 已验证
DOI
PubMed
高影响因子
PHARMACEUTICAL RESEARCH
·
2014
·Vol. 31
·期 11
·3038-3050
·
IF 3.952
Xu Huan,Zhang Wei,Li Yan,Ye Fei F.,Yin Peng P.,Yu Xiu,Hu Mei N.,Fu Yuan S.,Wang Che,Shang De J.
Purpose A novel bifunctional liposome with long-circulating and pH-sensitive properties was constructed using poly(2-ethyl-oxazoline)-cholesteryl methyl carbonate (PEtOz-CHMC) in this study. Methods PEtOz-CHMC was synthesized and characterized by TLC, IR and 1 H-NMR. The obtained PEtOz lipid was inserted into liposomes by the post-insertion method. Through a series of experiments, such as drug release, tumor cell uptake, cytotoxicity, calcium-induced aggregation, pharmacokinetic experiments, etc., the pH-sensitive and long-circulating properties of PEtOzylated liposomes was identified. Results PEtOz-CHMC modified liposomes (PEtOz-L) showed increased calcein release at low pH. Flow cytometric analysis results showed that the fusion and cellular uptake of PEtOz-L could be promoted significantly at pH 6.4 compared with those at pH 7.4. Confocal laser scanning microscope observations revealed that PEtOz-L could respond to low endosomal pH and directly released the fluorescent tracer into the cytoplasm. MTT assays in HeLa cells demonstrated that doxorubicin hydrochloride (DOX) loaded PEtOz-L exhibited stronger anti-tumor activity in a medium at pH 6.4 than in a medium pH 7.4. PEtOz-L remained stable when these liposomes were incubated in calcium chloride solution. The cumulative calcein release rate of PEtOz-L was significantly lower than that of CL when the liposomes were dialysed in PBS. The pharmacokinetic experiments of liposomes in rats showed that t
1/2 and AUC of PEtOz-L were 4.13 times and 4.71 times higher than those of CL. Conclusions PEtOzylated liposomes exhibits excellent long-circulating and pH-sensitive properties. Our results suggest that PEtOz is a promising biomaterial for the modification of liposome in drug delivery.
研究领域:
药剂学
·
生物材料科学
·
肿瘤学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）