MICROCHIMICA ACTA
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2019
·Vol. 186
·期 2
·1-11
·
IF 5.479
Long Ling,Liu Xiangjian,Chen Lulu,Li Dandan,Jia Jianbo
The authors report that CuO x /NiO y hollow nanocomposites are an effective bifunctional catalyst capable of oxidizing glucose and reducing hydrogen peroxide. Synthesis is based on a solvothermal process and subsequent thermal treatment. The structure can be controlled by adjusting the amounts of added NiCl 2 during the solvothermal etching process, and core-shell, yolk-shell or hollow structures can be obtained. The porous hollow structure composite of type CuO 30 /NiO 90 was used to modify a glassy carbon electrode. It exhibits excellent electrocatalytic activity towards glucose oxidation in solution of pH 13, typically at a working potential of +0.60 V (vs. Ag/AgCl). This enables voltammetric sensing of glucose with (a) a low limit of detection (0.08 μM, at S/ N = 3), (b) over a wide linear range (0.20 μM - 2.5 mM), and (c) high sensitivity (2043 μA·mM −1 ·cm −2 ). The sensor is reproducible, selective and stable. It can be used to detect glucose in spiked human serum. The CuO 30 /NiO 90 composite also displays good electrocatalytic activity towards reduction of H 2 O 2 in neutral aqueous medium, typically at an applied potential of −0.35 V. It has a detection limit of 90 nM, a sensitivity of 271.1 μA·mM −1 ·cm −2 , and a linear detection range that extends from 0.30 μM to 9.0 mM.
CuO x /NiO y nanocomposites with three different structures were synthesized by coordinated etching precipitation method. The hollow structure CuO 30 /NiO 90 was coated on the surface of glassy carbon electrode for the amperometric determination of glucose and hydrogen peroxide.
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RSC Advances
·
2019
·Vol. 9
·期 19
·10465-10472
·
IF 3.049
Guokang Fan,Peng Sun,Jie Zhao,Dongxue Han,Li Niu,Guofeng Cui
Precisely detecting the concentration of glucose in the human body is an attractive way to prevent or diagnose diabetes. Compared with the traditional enzyme-based electrochemical glucose sensors, the non-enzymatic ones have gradually come to people's attention recently. By integrating three electrodes into one device, glucose sensors can achieve superior performance and are convenient to carry. Herein, a non-enzymatic three-electrode interdigitated glucose sensor (TEIDGS) based on nanoporous gold is designed and fabricated. To our best knowledge, it is the first time that interdigitated electrodes are combined in a single non-enzymatic glucose sensor device. Due to the advantage of the interdigitated structure and the smart design of the three-electrode circuit board, the TEIDGS can effectively reduce concentration polarization and achieve a high detective sensitivity for glucose of 1217 μA mM−1 cm−2 and 343 μA mM−1 cm−2 in the ranges of 0.001–0.590 mM and 0.59–7.00 mM, respectively. Moreover, a low detection limit of 390 nM can be reached. In addition, this TEIDGS possesses excellent selectivity for glucose among other interferents. Strikingly, after three weeks of operation, it can still retain a high detection performance. This work will certainly provide an efficient structure and proper catalytic material choice for future non-enzymatic glucose sensors.
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ELECTROCHIMICA ACTA
·
2019
·
IF 5.383
Dingding Duan,Hua Yang,Yaping Ding,Li Li,Guohong Ma
A highly sensitive and selective molecular imprinting polymer (MIP) sensor was fabricated based on prussian blue (PB)-porous carbon (PC)-CNTs hybrids modified glassy carbon electrode (GCE) for enantioselective recognition of cysteine enantiomers . We prepared a conductive polypyrrole (PPy) MIPs by convenient electropolymerization methods and the elution of templates was performed by a simple overoxidation/dedoping process. The physical properties of materials and the modified sensors were investigated by using X-ray powder diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) and transmission electron microscopy (TEM). Cyclic voltammetry (CV), differential pulse voltammetry (DPV) and electrochemical impedance spectroscope (EIS) were used to assess the electrochemical performance of the sensors. Under optimal conditions, the resulted 3D porous conductive sensor was used to enantioselective recognition L-/D-cysteine respectively, with a linear detection range from 10 −13 to 10 −7 mol L −1 . Furthermore, good reproducibility, long-term stability, favorable selectivity was achieved in the experiment and the MIECS was successfully applied to detect cysteine in human serum with high recovery.
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SENSORS
·
2019
·Vol. 19
·期 3
·640
·
IF 3.031
Meng Li,Jing Wu,Haiping Su,Yan Tu,Yazhuo Shang,Yifan He,Honglai Liu
In this work, three different aqueous solutions containing imidazole-based ILs with different alkyl chain lengths ([Cnmim]Br,n= 2, 6, 12) were adopted as the medium for the synthesis of ionic liquid-polypyrrole (IL-PPy) composites. Herein, the ILs undertook the roles of the pyrrole solvent, the media for emulsion polymerization of PPy and PPy dopants, respectively. The electrochemical performances of the three IL-PPy composites on a glassy carbon electrode (GCE) were investigated by electrochemical experiments, which indicated that [C12mim]Br-PPy (C12-PPy) composites displayed better electrochemical performance due to their larger surface area and firmer immobilization on the GCE. Further, C12-PPy/GCE were decorated with Au microparticles by electrodeposition that can not only increase the conductivity, but also immobilize sufficient biomolecules on the electrode. Then, the obtained C12-PPy-Au/GCE with outstanding electrochemical performance was employed as a horseradish peroxidase (HRP) immobilization platform to fabricate a novel C12-PPy-Au-HRP/GCE biosensor for H2O2detection. The results showed that the prepared C12-PPy-Au-HRP/GCE biosensor exhibited high sensitivity, fast response, and a wide detection range as well as low detection limit towards H2O2. This work not only provides an outstanding biomolecule immobilization matrix for the fabrication of highly sensitive biosensors, but also advances the understanding of the roles of ILs in improving the electrochemical performance of biosensors.Keywords:ionic liquids (ILs);polypyrrole (PPy);gold particles;horseradish peroxidase (HRP);enzyme immobilization;hydrogen peroxide sensor
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TALANTA
·
2019
·
IF 4.916
Yusheng Wang,Xiaoqi Wang,Wei Lu,Qingpan Yuan,Yourong Zheng,Bo Yao
In this work, a thin film PET based gold electrode (PGE) glucose sensor was fabricated through ultraviolet mediated chemical plating technique. Compared with the most existing wearable thin film gold electrode sensors, the PGE-glucose sensor is simple, low cost and minimum instrumentation was required. The proposed glucose sensor revealed a sensitivity of 22.05 μA mM −1 cm −2 in a linear range from 0.02 to 1.11 mM with a low detection limit of 2.7 μM (S/N = 3). The PGE-glucose sensor had a good selectivity and was not interfered by lactic acid, urea, acetaminophen, uric acid , dopamine and ascorbic acid . Additionally, it exhibited good reproducibility and long-term stability over four weeks. Finally, the disposable PGE-glucose sensor was successfully applied to determine the glucose in sweat and commercial beverage accurately.
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Materials Express
·
2019
·Vol. 9
·期 5
·444-450
·
IF 1.465
Lai Yuxuan,Huang Hao,Xia Zhihuan,Li Song,Deng Yan,Liu Xueying
A sandwich-type electrochemical immunosensor was developed in this study for sensitive detection of carcinoembryonic antigen (CEA) based on rGO/CS/AuNPs as a sensing platform and PTh-Au composites as signal labels. Reduced graphene oxide (rGO), Chitosan (CS) and AuNPs were layer by
layer modified on glass carbon electrode (GCE), which effectively enhanced electron transfer and provided excellent large electrochemical active surface area. Polythionine-Au composites prepared by chemical reduction method greatly amplified electrochemical signal with high conductivity and
large surface area. The prepared immunosensor showed high sensitivity, good stability and selectivity for detection of CEA with a wide range of 0.3 ng/mL to 30 ng/mL (R
2 = 0.9972) and a detection limit as low as 0.1471 pg/mL. Furthermore, this sandwich-type immunosensor might
be as a versatile platform for reliable early cancer diagnostics clinical.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2019
·
IF 2.931
Zhao,Jianhao,Yaling
The application of fluorescent carbon dots in bio-imaging has huge positive significance in the field of biomedicine. By taking this advantage, herein we prepared nitrogen, sulfur and iodine doped carbon dots (N,S,I-CDs) by a facile hydrothermal reaction using C 3 N 3 S 3 , potassium iodate (KIO 3 ) and ethylenediamine (EDA), and the obtained N,S,I-CDs show bright blue fluorescence with a high fluorescence quantum yield of about 32.4%. The prepared N, S, I-CDs could interact with the folic acid (FA) with high selectivity, lead to development of a high sensitive method for the FA detection from 0.1 to 175 μM wide linear range with a detection limit of 84 nM (S/N = 3) and also applied them in U-2 OS cells imaging. Moreover, this sensor possessed a good sensitivity, linearity and reversibility in the temperature range of 10–80 °C, and successfully applied for the temperature sensing in cell HT-29 samples. This investigation illustrates that as-prepared N, S, I-CDs probe may have great potential as a high-performance platform for the accurate recognition of temperature in cells and could provide a new tool for the detection of FA in cells.
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JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2019
·Vol. 166
·期 10
·B814
·
IF 3.12
Jinlei Huang,Xufei Fang,Xue Liu,Siyuan Lu,Shuxin Li,Zixuan Yang,Xue Feng
Fast and accurate measurement of hydrogen peroxide is essential to glucose monitoring. In this work, we describe a highly efficient H2O2 sensor through the modification of Prussian blue (PB) on the nanoporous gold film (NPG). By de-alloying from Au-Ag film, we prepare the NPG film and characterize it through SEM and AFM. Tests using chronoamperometry and cyclic voltammetry on the Prussian blue modified NPG electrodes show an excellent electrochemical performance of the sensors. Owing to the comparatively huge specific surface area in the nanoporous structure, such electrodes significantly enhance the direct electron transfer from PB nanocrystals to NPG film. Comparing to earlier PB modified electrodes, the sensor fabricated in this work exhibits the widest linearity of 0.001∼17 mM, and produces a high selectivity of 708 μA·cm−2·mM−1.
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TALANTA
·
2019
·
IF 4.916
Xiao-Dong Sun,Hai-Long Wu,Zhi Liu,Yue Chen,Jun-Chen Chen,Li Cheng,Yu-Jie Ding,Ru-Qin Yu
Chemometrics multivariate calibration coupled with high performance liquid chromatography-diode array detection (HPLC-DAD) analytical strategy was applied for fast and sensitive quantification of the eight small molecules (uric acid, creatinine, tyrosine, homovanillic acid , hippuric acid, indole-3-acetic acid, tryptophan and 2-methylhippuric acid) in human urine. The objective of this work was to get the successful resolution of the complex matrix with minimum experimental time in the presence of highly overlapping peaks, of distortions in the time and baseline aspects among chromatograms, and of the presence of unknown and background interferences. All the analysis were based on a short C18 column with the chromatographic system operating in isocratic mode and all analytes can be successfully quantified within 6 min. The second-order HPLC-DAD data acquired were handled intelligently by two typical chemometrics tools including alternating trilinear decomposition (ATLD) and multivariate curve resolution-alternating least squares (MCR-ALS). Reasonable resolution and satisfactory quantification results were obtained regardless of the complex matrix interferences from the urine samples and the second-order advantage was fully exploited. With the validation by classic HPLC method, the proposed strategy could take extra advantages such as increased selectivity and sensitivity, shorter analysis time, undemanding elution conditions and sufficiency of lower limit of quantification benefit from multivariate calibration. The method was shown as a promising means for fast and sensitive determination of small molecules in human urine and also for fast diagnosis or surveillance in related diseases.
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ACS Applied Materials & Interfaces
·
2019
·
IF 8.456
Xing Qing,Yuedan Wang,Yang Zhang,Xincheng Ding,Weibing Zhong,Dong Wang,Wenwen Wang,Qiongzhen Liu,Ke Liu,Mufang Li,Zhentan Lu
Fiber-based organic electrochemical transistors (FECTs) provide a new platform for the realization of an ultrafast and ultrasensitive biosensor, especially for the wearable dopamine (DA)-monitoring device. Here, we presented a fully filament-integrated fabric, it exhibited remarkable mechanical compatibility with the human body, and the minimum sensing unit was an organic electrochemical transistor (OECT) based on PVA-co-PE nanofibers (NFs) and polypyrrole (PPy) nanofiber network. The introduction of NFs notably increased the specific surface area and hydrophilicity of the PA6 filament, resulting in the formation of a large area of intertwined PPy nanofiber network. The electrical performance of PPy nanofiber network-modified fibers improved considerably. For the common FECTs, the typical on/off ratio was up to two orders of magnitude, and the temporal recovery time between on and off states was shortened to 0.34 s. Meanwhile, the device exhibited continuous cycling stability. In addition, the performances of FECT-based dopamine sensors depending on different gate electrodes have also been investigated. The PPy/NFs/PA6 filament-based dopamine sensor was more superior to the gold and platinum (Pt) wires, and the sensor presented long-term sensitivity with a detection region from 1 nM to 1 μM, rapid response time to a set of DA concentrations, remarkable selectivity in the presence of sodium chloride, uric acid, ascorbic acid and glucose, and superior reproducibility. Moreover, it could also be woven into the fabric product. The novel and wearable FECT device shows the potential to become the state-of-the-art DA-monitoring platform.
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Biomolecules
·
2019
·Vol. 9
·期 7
·294
·
IF 4.694
Yaling Tian,Peihong Deng,Yiyong Wu,Ziyu Ding,Guangli Li,Jun Liu,Quanguo He
In this paper, a tryptophan (Trp) molecularly imprinted chitosan film was prepared on the surface of an acetylene black paste electrode using chitosan as the functional polymer, Trp as the template molecule and sulfuric acid as the crosslinking agent. The surface morphologies of non-imprinted and imprinted electrodes were characterized by scanning electron microscopy (SEM). The formation of hydrogen bonds between the functional polymer and the template molecule was confirmed by infrared spectroscopy. Some factors affecting the performance of the imprinted electrode such as the concentration of chitosan, the mass ratio of chitosan to Trp, the dropping amount of the chitosan-Trp mixture, the solution pH, and the accumulation potential and time were discussed. The experimental results show that the imprinted electrode exhibit good affinity and selectivity for Trp. The dynamic linear ranges of 0.01–4 μM, 4–20 μM and 20–100 μM were obtained by second derivative linear sweep voltammetry, and the detection limit was calculated to be 8.0 nM. The use of the imprinted electrode provides an effective method for eliminating the interference of potentially interfering substances. In addition, the sensor has high sensitivity, reproducibility and stability, and can be used for the determination of Trp in pharmaceutical preparations and human serum samples.Keywords:molecularly imprinted chitosan film;electrochemical sensor;tryptophan;selective determination
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JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2019
·
IF 3.218
Peijin Wang,Qian Wu,Caixia Wang,Xue Yang,Min Wei,Yuxin Pu,Mao Zhou,Mingxiao Zhang
In this work, Prussian blue nanocubes functionalized partial reduced graphene oxide (PBs-PrGO) was first employed as a new electrochemiluminescence (ECL) signal amplification strategy of perylenetetracarboxylic acid (PTCA), and the ECL signal of PBs-PrGO-PTCA composite presented 3-fold enhancement compared with PTCA. The Fe III and Fe II active site of Prussian blue nanocubes (PBs) could promote the reduction of K 2 S 2 O 8 to generate more sulfate radical anion (SO 4 − ), which enhanced the ECL signal of PTCA. The partial reduced graphene oxide (PrGO) not only used as the substrate for in-situ growing PBs, but also could assemble PTCA through π–π stacking. Besides, folic acid (FA) could effectively quench the ECL signal of PTCA. Based upon above, a rapid and effectual FA sensor was constructed. Under the optimum conditions, the ECL sensor exhibited an excellent linear relationship for FA determination with the limit of detection low as 0.719 nM. The constructed sensor applied in the determination of FA in tablets and human urine with satisfactory results, which could realize rapid and accurate FA analysis.
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