JOURNAL OF APPLIED MICROBIOLOGY
·
2025
·Vol. 136
·期 11
·
IF 3.2
Ling Lijun,Zhang Wenyue,Mo Rongxiu,Kong Fanjin,Jiang Yijuan,Feng Lijun,Li Yao,Yue Rui,Zhou Yongpeng
AimsLanzhou lily (Lilium davidli var. unicolor) possesses both nutritional and medicinal value, however, its bulbs are highly susceptible to postharvest physical damage. Pathogenic bacteria readily infiltrate through these wounds, resulting in extensive decay and consequent economic losses. This study demonstrates that optimized volatile organic compound (VOC) combinations can significantly reduce effective antimicrobial concentrations and broaden the antimicrobial spectrum through synergistic effects, while exhibiting excellent preservation potential.Methods and ResultsFrom decayed lily bulbs, this study isolated and identified two bacterial strains, Bacillus cabrialesii SH-3 and Bacillus amyloliquefaciens SH-5, both exhibiting opportunistic pathogenicity. Fifteen binary VOC combinations were evaluated using fractional inhibitory concentration index (FICI), identifying QM (1/8 MIC 2-methylbutyric acid + 1/8 MIC 3- methylpentanoic acid, FICI = 0.25) and QH (1/2 3-methylpentanoic acid + 1/8 3-hepten-2-one, FICI = 0.625) as optimal formulations. Mechanistic studies revealed these combinations disrupt bacterial membrane integrity, induce protein/nucleic acid leakage, and trigger oxidative stress and metabolic dysfunction.ConclusionsAgainst SH-3, only QM showed synergistic effects, reducing required concentrations by 87.5% compared to individual minimum inhibitory concentration (MIC). For SH-5, all combinations demonstrated additive effects, with QH achieving potent inhibition at 50% and 87.5% reduced concentrations for respective components. Applied at MIC levels, both formulations significantly suppressed postharvest decay while maintaining bulb quality.
✓ 已验证
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2026
·
IF 6.7
Jiaqi Fu,Lianghua Xie,Zhen Zong,Khairiah M. Alwutayd,Hany A. El-Shemy,Wei Chen
Abstract Cyanidin-3-O-rhamnoside (C3R) and cyanidin-3-O-glucoside (C3G) show strong anti-inflammatory and antioxidant activities with great potential for chronic disease prevention. However, C3R biosynthesis from (+)-catechin has not been reported, and C3G production from this substrate is low for industrial applications. Here, we constructed a microbial platform for the efficient C3R and C3G production via genetic engineering and metabolic optimization. We screened glucosyltransferases and rhamnosyltransferases from various plants to identify the optimal combinations for C3G and C3R biosynthesis. Tuning UDP-glucose and UDP-rhamnose balance by plasmid copy number increased the C3R concentration by 120%. Overexpression of MdtH and yadH improved extracellular C3R and C3G accumulation by 16% and 31%, respectively. After optimizing substrate and culture conditions, the engineered strains produced 529.01 mg/L of C3R and 926.47 mg/L of C3G, with C3R synthesis reported for the first time. This study underscores the key roles of glycosyltransferases and UDP-sugar modulation, providing a versatile strategy for producing other disaccharide- or rhamnose-containing natural products.
✓ 已验证
DOI
高影响因子
JOURNAL OF CHEMICAL AND ENGINEERING DATA
·
2021
·
IF 2.694
Huan Ye,Ying Yu,Sile Shi,Chi Cai,Haoran Liu,Weiping Luo
In this study, the solid–liquid equilibrium (SLE) data of 3-methyl-2-nitrobenzoic acid (3-M-2-NBA), 3-methyl-4-nitrobenzoic acid (3-M-4-NBA), and 5-methyl-2-nitrobenzoic acid (5-M-2-NBA) in n-butanol, iso-butanol, tert-butanol, and sec-butanol were determined using the method of dynamic laser at atmospheric pressure. The experimental SLE data indicate that within the temperature range studied (290.15–348.95 K), the solubilities of 3-M-2-NBA, 3-M-4-NBA, and 5-M-2-NBA in the above four solvents are proportional to the temperature. The molar fraction of solubility of the same solute in different solvents from low to high is as follows: iso-butanol < n-butanol< sec-butanol < tert-butanol. The molar fraction of solubility of the three solutes in the same solvent from low to high is as follows: 3-M-4-NBA < 3-M-2-NBA<5-M-2-NBA. The experimental data were correlated using the Apelblat model, λh model, and NRTL activity coefficient model, and the calculated solubilities agree satisfactorily with the measured results. The best model for every system was selected using the result of Akaike information criterion analysis.
✓ 已验证
产品关联
DOI
高影响因子
MOLECULES
·
2023
·Vol. 28
·期 8
·3616
·
IF 4.6
Liqun Zhang,Pinggu Wu,Xiaoling Xiang,Dajin Yang,Liyuan Wang,Zhengyan Hu
Refined and deodorized camellia oil has been reported to contain a high amount of 3-monochloropropane-1,2-diol esters (3-MCPDE) due to the high-temperature deodorization step. To reduce 3-MCPDE in camellia oil, the physical refining process of camellia oil was simulated on a laboratory scale. Response surface methodology (RSM) was designed to modify and optimize the refining process with five processing parameters (water degumming dosage, degumming temperature, activated clay dosage, deodorization temperature and deodorization time). The optimized new refining approach achieved a 76.9% reduction in 3-MCPDE contents, in which the degumming moisture was 2.97%, the degumming temperature was 50.5 °C, the activated clay dosage was 2.69%, the deodorizing temperature was 230 °C, and the deodorizing time was 90 min. A significance test and analysis of variance results demonstrated that the deodorization temperature and deodorization time contributed significantly to the reduction of 3-MCPD ester. The joint interaction effects of activated clay dosage and deodorization temperature were significant for 3-MCPD ester formation.Keywords:3-MCPD esters;response surface analysis;optimized refining process parameters;reduction of 3-MCPD
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Polymer Chemistry
·
2016
·Vol. 7
·期 39
·6120-6132
·
IF 5.687
Cai Wang,Yudong Zheng,Yi Sun,Jinsheng Fan,Qiujing Qin,Zhenjiang Zhao
A novel block polyurethane was designed and synthesized based on poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), 4,4′-diphenylmethanediisocyanate (MDI) and poly(ethylene glycol) (PEG). The obtained polyurethanes were characterized by proton nuclear magnetic resonance (1H NMR), Fourier transform infrared spectroscopy (FTIR), atomic force microscopy (AFM), differential scanning calorimetry (DSC) and wide angle X-ray diffraction (WAXD). Furthermore, the biodegradability of the synthesized polyurethanes was evaluated at 37 °C in phosphate buffer solution (PBS) at pH 7.4. The results showed that these PHBV-based polyurethanes were degradable in PBS, as indicated by weight loss measurements, scanning electron microscopy (SEM) and FTIR. The cytotoxicity of the novel polyurethane was evaluated using the cell counting kit-8 (CCK8) assay in vitro. Preliminary evaluation of hemocompatibility was carried out via the hemolysis test, dynamic blood coagulation time and platelet adhesion. The current work provides a promising approach to prepare nontoxic and biodegradable polyurethanes with excellent mechanical properties and good hemocompatibility. This new material may find potential application in blood vessel tissue engineering.
✓ 已验证
产品关联
DOI
高影响因子
ORGANIC & BIOMOLECULAR CHEMISTRY
·
2014
·Vol. 12
·期 46
·9366-9374
·
IF 3.487
Ting-Ting Feng,Xuan Huang,Xiong-Li Liu,De-Hong Jing,Xiong-Wei Liu,Feng-Ming Guo,Ying Zhou,Wei-Cheng Yuan
The first phase transfer-catalysed direct γ-substitution of Morita–Baylis–Hillman carbonates of isatins with 3-substituted oxindoles has been developed, which affords 3-alkenyl-oxindole ring-fused 3,3′-disubstituted oxindoles in up to 83% yield under mild reaction conditions. Furthermore, their biological activity has been preliminarily demonstrated by in vitro evaluation against human prostate cancer cells PC-3 and human leukemia cells K562, using MTT-based assays with the commercially available standard drug Cisplatin as a positive control. Gratifyingly, compounds 3aa, 3ba and 3ca exhibited comparable in vitro inhibitory activities against human prostate cancer cells (PC-3) to Cisplatin. What's more, 3ba also had a good inhibition ability against human leukemia cells K562. These results indicate that 3-alkenyl-oxindole ring-fused 3,3′-disubstituted oxindole analogs may be potential lead compounds for further biological screening.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ELECTROCHIMICA ACTA
·
2013
·
IF 3.777
Xuemin Chen,Tongqing Ren,Ming Ma,Zhengguo Wang,Guoqing Zhan,Chunya Li
Using carboxylic acid-functionalized single walled carbon nanotubes (SWCNTs-COO − ) as an anion and 3-butyl-1-[3-( N -pyrrolyl)propyl]imidazolium as a cation, a novel SWCNTs-COO-ionic liquid (SWCNTs-COO-IL) nanocomposite was fabricated successfully. The as-prepared SWCNTs-COO-IL nanocomposite was confirmed with transmission electron microscopy, X-ray photoelectron spectroscopy, UV–vis, FTIR and Raman spectroscopy. The SWCNTs-COO-IL nanocomposite was coated onto a glassy carbon electrode surface followed by cyclic voltammetric scanning to fabricate a SWCNTs/poly{3-butyl-1-[3-( N -pyrrolyl)propyl] imidazolium ionic liquid} composite film modified electrode (SWCNTs/Poly-IL/GCE). Scanning electron microscope and electrochemical impedance spectroscopy were used to characterize SWCNTs/Poly-IL/GCE. Electrochemical behaviors of bisphenol A (BPA) at the SWCNTs/Poly-IL/GCE were investigated thoroughly. It was found that an obvious oxidation peak appeared without reduction peak in the reverse scanning, indicating an irreversible electrochemical process. The oxidation peak currents of BPA were linearly related to scan rate in the range of 20–300 mV s −1 , suggesting an adsorption controlled process rather than a diffusion controlled process. Differential pulse voltammetry was employed for the voltammetric sensing of BPA. Experimental conditions such as film thickness, pH value, accumulation potential and time that influence the analytical performance of the SWCNTs/Poly-IL/GCE were optimized. Under optimal conditions, the oxidation peak current was linearly related to BPA concentration in the range of 5.0 × 10 −9 to 3.0 × 10 −5 mol L −1 with a detection limit of 1.0 × 10 −9 mol L −1 ( S / N = 3). The developed method was successfully employed for the determination of BPA leaching from plastic drinking bottle.
✓ 已验证
产品关联
DOI
高影响因子
Current Protocols
·
2023
·Vol. 3
·期 5
·e741
·
IF 0
Wen-Wu Sun,Yi-Bing Xie,Ting-Ting Deng,Jie Huang,Ji-Kai Liu,Bin Wu
Despite the importance of nucleosides and nucleotides for drug discovery, only a few practical methods to prepare tricyclic nucleosides have been reported. Here, we describe a synthetic strategy for late-stage functionalization of nucleosides and nucleotides via chemo- and site-selective acid-promoted intermolecular cyclization. The nucleoside analogs with an additional ring were obtained in moderate-to-high yields, including some antiviral drugs (acyclovir, ganciclovir, and penciclovir) derivatives, endogenous fused ring nucleoside (M 1 dG) and its derivatives, and nucleotide derivatives. © 2023 Wiley Periodicals LLC. Basic Protocol 1 : Synthesis of tricyclic acyclovir analogs ( 3a-3c ) Basic Protocol 2 : Synthesis of tricyclic nucleosides M 1 dG ( 6 ) and M 1 G ( 9 ) Basic Protocol 3 : Synthesis of tricyclic nucleotide ( 12 )
研究领域:
药物化学
·
有机合成
·
核酸化学
·
分子生物学
·
化学生物学
·
抗病毒药物研发
✓ 已验证
DOI
POLYMER TESTING
·
2021
·
IF 4.282
Haochuan Luo
An effective method to prepare crosslinked polybenzimidazole (PBI) via [2 + 3] Huisgen cycloadditions has been proposed. Propargyl modified PBI (PBI-PG) has been synthesized firstly. In the presence of diazido crosslinkers, PBI-PG can be thermally crosslinked by [2 + 3] Huisgen cycloadditions. Evidences from 1 H NMR and ATR spectra imply a small amount of grafting alkenyl structures on PBI-PG. The grafting alkenyl structures provide another crosslinking mechanism named as alkyne-alkene crosslink. The alkyne-alkene crosslink is highly efficient under heat to form crosslinking networks which display toughness, low tensile strengths, and large swelling ratios in phosphoric acid (PA) solutions. Influences of rigidities and chain lengths of crosslinkers on performances of crosslinked PBIs have been investigated. PBIs crosslinked by rigid crosslinkers behave as brittle materials while that crosslinked by flexible crosslinkers behave as tough materials. Addition of crosslinkers leads to decreased gel fractions but improved tensile strengths, which is attributed to competition results of crosslinking and plasticizing effects. PBI-R2-20, which is crosslinked by a long rigid crosslinker, exhibits the highest tensile strength of 102.33 MPa, which is 66.34 MPa higher than that of PBI-PG. With even lower PA uptakes, PBIs crosslinked by rigid crosslinkers display more thermally stable and higher conductivities than that crosslinked by flexible crosslinkers. After doping in 12 mol/L PA solution, PBI-R2-20, with an acid uptake of 163 wt%, displays the highest tensile strength of 8.1 MPa and the most thermally stable and the highest conductivity which reaches a maximum of 2.30 × 10 −2 S cm −1 at 188 °C among all crosslinked membranes.
研究领域:
材料科学
·
化学工程
✓ 已验证
DOI
高影响因子
JOURNAL OF THE AMERICAN CERAMIC SOCIETY
·
2021
·Vol. 104
·期 6
·2721-2729
·
IF 3.784
Xianju Zhou,Jie Zhang,Lingni Chen,Yanhong Li,Guotao Xiang,Sha Jiang,Li Li,Xiao Tang,Zhongmin Cao,Guangxin Xie
A series of novel red emission phosphors Sr 3 Lu 1- x (VO 4 ) 3 : x Eu 3+ ( x = 0.007, 0.009, 0.02, 0.04, 0.06) were synthesized successfully by traditional high-temperature solid-state reaction. The results of X-ray diffraction (XRD) reveal the doped Eu 3+ ions have replaced the lattice sites of Lu 3+ ions. The diffuse reflectance spectra illustrate the energy gap of Sr 3 Lu(VO 4 ) 3 host is 3.61 eV. The room-temperature steady-state fluorescence spectra show that these phosphors can be effectively pumped by the charge-transfer band (CTB) of the host in near ultraviolet (NUV) spectral region and then produce strong and pure red emission at 615 nm originated from 5 D 0 → 7 F 2 electric dipole transition of Eu 3+ . The Commission Internationale de L’Eclairage (CIE) coordinates of Sr 3 Lu 0.96 (VO 4 ) 3 :0.04Eu 3+ are (x = 0.65, y = 0.35), which are very close to the red standard of National Television Standards Committee NTSC (0.67, 0.33). The fabricated warm white-light-emitting diodes (LED) demonstrate high color-rendering index Ra as 93. The results imply the red-emitting Sr 3 Lu(VO 4 ) 3 :Eu 3+ phosphors could be potentially utilized in the fields of solid-state lighting.
研究领域:
材料科学
·
光学工程
✓ 已验证
DOI
高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2019
·Vol. 30
·期 7
·6760-6767
·
IF 2.195
Bao Runxi,Li Mingyu,Shen Meng,Liu Huan,Zhang Guangzu,Zeng Yike,Jiang Shenglin
Ceramic-polymer pyroelectric composite materials with favorable flexibility hold great potential for wearable sensors and energy harvesters, and the further improvement in pyroelectric properties would push them closer to practical application. In this work, an uniaxial stretching method was proposed to optimize the performance of lead zirconate titanate nanowires (PZT NWs)/polyvinylidene fluoride (PVDF) 1–3 composites. With just one stretching treatment, the pyroelectric coefficient of the composite was enhanced by inducing more β phase in PVDF matrix, and the permittivity was decreased by reorientation of PZT NWs to the strain direction in the meantime. As a result of the incorporated effects, the figure of merit of the stretched composite with orientated PZT NWs increases to the value of 2.18 × 10 −10 C cm −2 K −1 , which is 619% higher than that of untreated samples at 35 °C. This work could provide an efficient approach to fabricate high-performance ceramic-polymer pyroelectric composite materials.
研究领域:
材料科学
·
能源技术
✓ 已验证
DOI
高影响因子
Crystals
·
2022
·Vol. 12
·期 10
·1425
·
IF 2.67
Zijie Xiao,Tingting Tao,Jingting Shu,Wei Dang,Shusheng Pan,Wei Zhang
Studying the carrier recombination process in MAPb(Br1−yIy)3single crystals (SCs) is important for its application in the optoelectronic field. In this work, a series of MAPb(Br1−yIy)3SCs with varied Br/I compositions have been studied. Steady-state photoluminescence (PL) spectra, time-resolved photoluminescence (TRPL) spectra and time-resolved microwave photoconductivity (TRMC) were used to understand the radiative and non-radiative recombination processes of MAPb(Br1−yIy)3SCs. By comparing the dynamics of TRPL and TRMC, we conclude that the dynamics of TRPL is dominated by the electron trapping process, which is in accordance with the fast decay component of TRMC kinetics, whereas the slower decay component in TRMC is dominated by the hole trapping process. Moreover, we find both the electron and hole trapping rates in mixed-halide perovskite MAPb(Br1−yIy)3(0 < y < 1) SCs are higher than that of mono-halide perovskite MAPbBr3SCs and MAPbI3SCs. This suggests mixed-halide crystals could introduce additional electron and hole trapping densities, which could be related to the fluctuation of Br/I compositions in the crystals. This work is helpful for understanding carrier recombination process in mixed-halide perovskite SCs.
研究领域:
材料科学
·
光学
·
半导体物理
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）