ANALYTICAL BIOCHEMISTRY
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2023
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IF 2.9
Qinhong Yin,Xiaolan Yang,Lihua Yang,Dezhi Yang,Yaling Yang,Yanqin Zhu
As newly developed synthetic enzymes with exceptional catalytic capabilities and outstanding stability, nanozymes have drawn considerable interest in the realm of sensing. Using a simple hydrothermal process, iodine and copper-doped carbon dots (Cu,I-CDs) with simulated enzymes were fabricated in the current investigation. Cu,I-CDs demonstrate peroxidase-mimicking function together with high catalytic effectiveness due to aforementioned features. This led to generation of a colorimetric sensor for quick and accurate quantitative assessment of morphine (MOR). The outcomes showed the method's usefulness for the colorimetric detection of MOR. The linear range for MOR detection is 0.25–25 μg/mL having a reduced detection limit of 64 ng/mL. This sensor's successful use in the analysis of MOR in biological material is more noteworthy.
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DYES AND PIGMENTS
·
2023
·
IF 4.5
Tongsheng Huang,Xin Hu,Shiyu Huang,Dong Liu,Lihua Chen,Qinghua Wu,Weifeng Zhu,Xiaoyong Zhang,Meiying Liu,Yen Wei
Photodynamic therapy (PDT) is a promising treatment regime for cancer therapy with some advantages such as minimal invasiveness, high spatiotemporal selectivity, and negligible drug resistance. Developing Type I photosensitizer (PS) is a viable strategy to overcome the limitations of conventional O 2 -dependent Type Ⅱ PS, whereas there are still significant challenges. Herein, we report the synthesis of β -cyclodextrin-based fluorescent hyperbranched polymer ( β -CD-HPG-DEP) through a one-step anionic ring-opening polymerization between β -cyclodextrin ( β -CD), glycidol and a fluorescent molecule DEP. The potential utilization of β -CD-HPG-DEP as the agent for cancer phototheranostics was also investigated in detail. The results demonstrated that β -CD-HPG-DEP possesses excellent water dispersibility, good biocompatibility, high reactive oxygen species (ROS) generation efficiency mainly via Type I mechanism, and extraordinary fluorescence imaging capability. Meanwhile, β -CD-HPG-DEP has great potential to serve as drug delivery vehicle since the cyclodextrin unit has many hydroxyl groups, thus providing a promising strategy for designing and fabrication of multifunctional theranostics. Considering the simple synthesis route, ready designability and multifunctional potential of anionic ring-opening polymerization, as well as good biocompatibility of resultant polymer, we believe the strategy developed in this work could also be a rational route to synthesize multifunctional composites for various biomedical applications.
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BIOSENSORS & BIOELECTRONICS
·
2021
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IF 10.618
Lu Cao,Hongguang Liu,Wenjun Xie,Shumeng Jiao,Xueqiang Wu,Kaisong Yuan,Xia Zhou,Muzi Yang,Yanyan Guan,Huaihong Cai,Zhihui Lai,Jian Chen,Haibo Zhou
With the increasing number of reports on aristolochic acid I (AAI), more and more toxic and side effects have been discovered successively. The main recognized carcinogenic mechanism is that AAI is metabolized into aristololactam I (AAT) in the body by nitroreductases , ultimately forming AAT-DNA adducts that cause disease. However, the carcinogenic mechanism is still not well understood by currently reported indirect method, there has always been a great demand to develop a direct method for real-time monitoring such process. In this work, surface-enhanced Raman spectroscopy (SERS) was used for the first time to monitor the process of AAI under the action of reducing agent sodium borohydride and catalyst Raney nickel to form AAT. We first found the abundant intermediate product-amino derivative of AAI, which was never reported before by other methods. The AAT was then obtained by a one-step dehydration reaction from the amino derivative of AAI under such reduction conditions. The product of amino derivative of AAI and AAT were further verified by thin-layer chromatography, H nuclear magnetic resonance spectra , mass spectrometry, and ultra-high performance liquid chromatography. Furthermore, a density functional theory-supported in-depth vibrational characterization of AAI and AAT was performed. The monitoring of the AAI reduction process by SERS can be of great significance for further exploration of its pathogenic mechanism, prevention, and monitoring of “nephropathy” and other diseases caused by AAI.
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JOURNAL OF FOOD SCIENCE
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2021
·Vol. 86
·期 6
·2457-2467
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IF 3.167
Guilan Yang,Song Qin,Wenjun Li
Hydrolysates containing angiotensin I-converting enzyme (ACE)-inhibitory peptide were prepared from protein of Alaska pollack skins using alcalase and trypsin. The protein hydrolysate was separated by ultrafiltration, Sephadex G-25 gel filtration chromatography and reversed phase high-performance liquid chromatography (HPLC), from which a novel purified peptide was obtained. Both random coil structure and β-sheet in the purified peptide were revealed in Fourier transform infrared spectrum. The amino sequence of the purified peptide was identified as GPLGVP, VLYPVK, VFLENVLR, and FEEF by HPLC-Q-TOF-MS (HPLC-quadrupole time-of-flight mass spectrometry). The peptide GPLGVP whose molecular weight was 538.31 Da showed the highest ACE inhibitory activity (IC 50 = 105.8 µM). The purified peptide featured a noncompetitive inhibition kinetic mechanism was shown in the Lineweaver–Burk plots and was susceptible to enzymes as indicated in the studies on stability of gastrointestinal proteases. Moreover, the peptide GPLGVP can combine ACE catalytic pocket through hydrogen bonds and other forces with high binding power as disclosed in molecular docking simulation, which provides the inhibitory effect of GPLGVP on ACE.
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TALANTA
·
2021
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IF 6.057
Tongtong Li,Yan Zhang,Xiaohong Sun,Yanjin Zhang,Yongan Wang,Zhiyong Nie
A fluorescent probe was developed for ratiometric detection of thallium ions in mineral water samples by modifying a G-rich aptamer (PS2.M − 7) with a fluorescence donor (Cyanine-3, Cy 3 ) and a quencher (Cyanine-5, Cy 5 ). The probe had a random coil structure that changed into a G-quadruplex structure upon binding with Tl + . This change in structure decreased the distance between the donor and acceptor moieties, which resulted in fluorescence resonance energy transfer between Cy 3 and Cy 5 . Under optimized conditions, the limit of detection and linear concentration range for Tl + were 30.1 μM (3σ) and 10 μM–10 mM ( R 2 = 0.9981), respectively. This simple and cost-effective fluorescence sensor provided satisfactory results for detection of thallium ions in spiked mineral water samples.
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COLLOIDS AND SURFACES B-BIOINTERFACES
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2020
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IF 4.389
Mengyue Pei,Rong Xu,Chuangnian Zhang,Xiaoli Wang,Chen Li,Yunzhang Hu
Cancer immunotherapy is an important tumor therapy option for prevention and treatment of tumors and has attracted tremendous interests. However, the therapeutic outcomes are limited by insufficient antigen uptake and presentation by antigen-presenting cells such as dendritic cells (DCs). In this study, mannose-functionalized antigen nanoparticles with endosome escape activity were designed for targeted DCs, accelerated endosomal escape and enhanced MHC-I antigen presentation for cancer immunotherapy. Mannose was selected as DCs targeting ligand to enhance antigen uptake. Model antigen ovalbumin (OVA) was directly conjugated with mannose to obtain DCs targeting antigen, which was then complexed with polyethylenimine (PEI) through electrostatic interaction to form mannose-functionalized antigen nanoparticles (MAN-OVA/PEI NPs). Flow cytometry analysis revealed that the MAN-OVA/PEI NPs greatly increased antigen uptake by DCs compared with OVA/PEI NPs. Confocal laser scanning microscopy further demonstrated that MAN-OVA/PEI NPs enhanced cytosolic antigen release. Moreover, MAN-OVA/PEI NPs significantly promoted cytokine production and DCs maturation in vitro . More importantly, MAN-OVA/PEI NPs treated DCs exhibited enhanced cross-presentation to B3Z T cell hybridoma in vitro . This work suggests that mannose-functionalized antigen nanoparticles provide a versatile delivery vehicle for targeted DCs, accelerated endosomal escape and enhanced MHC-I antigen presentation for cancer immunotherapy.
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INORGANIC CHEMISTRY
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2020
·
IF 4.825
Bo Ni,Hongzhi Cao,Chengkai Zhang,Shengli Li,Qiong Zhang,Xiaohe Tian,Dandan Li,Jieying Wu,Yupeng Tian
The construction of novel classes of photosensitizers for efficient reactive oxygen species (ROS) generation is of great interest, yet it is a challenge. In this work, a bis(terpyridine)zinc(II) complex (namely, ZnL1) with two-photon absorption activity as an efficient ROS photogenerator was synthesized. Benefiting from the coordinated Zn, the decreased singlet–triplet energy gap favors the intersystem crossing process facilitating the singlet oxygen (1O2) generation via energy transfer. In addition, it makes the superoxide radical (O2·–) generation easier. This is an extremely rare study on two-photon excited ROS generation by activating type I and type II processes based on a cheaper and bioaccessible Zn complex.
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CHEMICAL ENGINEERING SCIENCE
·
2020
·
IF 3.871
Chengzhen Sun,Mei Liu,Shijing Xu,Shaohua Zhu,Jiazhong Wu,Bofeng Bai
We experimentally study the influence of inorganic salt ions on the wettability of originally oil-wet rock surfaces, formed by adsorbing polar molecules via polar interactions. It is found that the ions can alter the surface to a more water-wet state with a reduction in the water contact angle of about 20° at most. At lower concentrations, the effect of CaCl 2 solutions is more obvious than that of NaCl solutions, and Na 2 SO 4 solutions are more effective than NaCl solutions. Through a series of characterization, underlying mechanisms are revealed as: (1) the octadecylamine protonates at oil–water interface and attracts with the anions, reducing oil–water interfacial tension; (2) the cations are adsorbed on the negatively charged quartz surface, weakening the electronegativity of quartz surface and replacing the adsorbed octadecylamine molecules. Finally, a quantitative theoretical model is established based on the Young equation to predict the dependence of contact angle on the ion concentration.
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MOLECULAR CRYSTALS AND LIQUID CRYSTALS
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2020
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IF 0.512
Chanchan Xu,Lu Lu,Le Lv,Jiandong Guo,Chaoyun Luo,Dawei Luo,Wei Liu
A new zero-dimensional (0D) silver (I) chloride based coordination complex 0 D-Ag2Cl2(L) (1, L = (oxybis(2,1-phenylene))bis(diphenylphosphine)) has been prepared by mixing the silver(I) salt and a bidentate P-ligand at room temperature in solution. 1exhibits a band gap of 3.5 eV and a decomposition temperature (Td) of 280 °C. Such a high Td for molecular complex is a result of the strong Ag-P bonding and the “chelating effect” of the metal of the bidentate P-ligand. It has been used as an efficient photocatalyst for the degradation of organic dyes in water.
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CHEMISTRY OF MATERIALS
·
2020
·
IF 9.567
Linyuan Lian,Moyan Zheng,Peng Zhang,Zhi Zheng,Kang Du,Wen Lei,Jianbo Gao,Guangda Niu,Daoli Zhang,Tianyou Zhai,Shengye Jin,Jiang Tang,Xiuwen Zhang,Jianbing Zhang
Self-trapped excitons (STEs) in metal halide materials are attracting an increasing level of interest due to their unique light emission properties. Light emission from STEs in metal halides is usually associated with excited-state structural deformation, which lowers the symmetry of local structures, as seen for the STEs in a wide range of materials systems. Here, we reveal a prototypic STE-associated structural “distortion” that, however, enhances the symmetry of local structures, in a series of all-inorganic copper(I)-based halides Cs3Cu2X5 (X = Cl, Br, or I). We further find that the emission peaks of Cs3Cu2X5 blue-shift when the halogen changes from Cl to Br to I, which is the opposite of the trends found in traditional halide perovskites. This phenomenon is attributed to a synergetic combination of the significant change in band gap associated with structural deformation and a strong excitonic effect. Due to the highly localized electron and hole upon photoexcitation, Cs3Cu2Cl5 shows an extremely long and temperature-sensitive photoluminescence (PL) lifetime among metal halide materials with STEs. Remarkably, strong green emission with a PL quantum yield exceeding 90% is found in Cs3Cu2Cl5, opening the way to designing light emission compounds based on local symmetry-enhancing STE mechanisms.
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CHEMOSPHERE
·
2020
·
IF 5.778
Lun Wang,Keming Wang,Ruihua Huang,Zhen Qin,Yingchun Su,Shanshan Tong
In the past few years, two-dimensional (2D) nanomaterials have emerged great potential for the removal of valuable metals and the capture of polluted-heavy metals. Herein, hierarchically flower-like microcrystals with 2D WS 2 nanosheets (F-WS 2 MCs) were prepared by one-pot hydrothermal synthesis strategy and its adsorption performances for precious metals were systematically assessed. The excellent adsorption efficiencies of ∼86.8%, ∼27.6%, and ∼94.1% towards Ag (I), Pd (II), and Au (III) respectively were achieved within 120 min, and the adsorption curves were in good agreement with a pseudo-second-order kinetic model showing a fast uptake rate at the optimum pH values (1.30 for Au (III), 1.43 for Ag (I), and 3.20 for Pd (II)). The adsorption isotherm followed well in the Langmuir model with the maximum removal capacities ( q max ) of 186.2 mg g −1 for Ag (I), 67.29 mg g −1 for Pd (II), and 1340.6 mg g −1 for Au (III), respectively. Furthermore, for recycling purposes, the relevant desorption solution was investigated with different ratios of monobasic acid and thiourea , suggesting the best desorption efficiency of 93.03%, 88.08%, and 85.34% for Ag (I), Pd (II), and Au (III), respectively. By characterizing the crystalline phase, and micromorphology element mapping of F-WS 2 MCs before and after adsorption, the strong affinity and significant adsorption–reduction were indicated to dominate the adsorption process. Therefore, this work broadens the application range of WS 2 microcrystals, providing an alternative material for capturing precious metals and wastewater treatment applications.
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JOURNAL OF BIOLOGICAL CHEMISTRY
·
2021
·Vol. 297
·期 4
·
IF 5.157
Ge Liu,Rui Liu,Yeqi Shan,Chaomin Sun
Many natural polysaccharides have significant anticancer activity with low toxicity, but the complex chemical structures make in-depth studies of the involved mechanisms extremely difficult. The purpose of this study was to investigate the effect of the marine bacterial exopolysaccharide (exopolysaccharide 11 [EPS11]) on liver cancer metastasis to explore the underlying target protein and molecular mechanism. We found that EPS11 significantly suppressed cell adhesion, migration, and invasion in liver cancer cells. Proteomic analysis showed that EPS11 induced downregulation of proteins related to the extracellular matrix–receptor interaction signaling pathway. In addition, the direct pharmacological target of EPS11 was identified as collagen I using cellular thermal shift assays. Surface plasmon resonance and pull-down assays further confirmed the specific binding of EPS11 to collagen I. Moreover, EPS11 was shown to inhibit tumor metastasis by directly modulating collagen I activity via the β1-integrin–mediated signaling pathway. Collectively, our study demonstrated for the first time that collagen I could be a direct pharmacological target of polysaccharide drugs. Moreover, directly targeting collagen I may be a promising strategy for finding novel carbohydrate-based drugs.
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